FastQCFastQC Report
Fri 27 May 2016
ERR765570_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765570_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13225286
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT450760.3408319487381974No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA442840.33484342039937737No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT343100.2594272819506512No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215720.16311178450129546No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG176120.13316914280719525No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG171910.12998584680890832No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155600.11765341029297968No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG149640.11314689149255448No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC138720.10488998120721171No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC136880.10349870694667775No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAG67300.038.4723361
GTACATA41650.031.7032171
GTACAAA62250.028.0811961
GTACTAG47900.024.917831
AATACGG91200.024.5759835
TACAAGG69600.024.2375642
TACATAG36400.024.0112822
GTATCAA989100.022.0297161
GTAACAA46450.021.9526021
TAATACG109900.021.933624
CAACACA188950.021.3118575
TTAATAC125850.021.24533
ACGGACC113350.021.057178
GTATAAG53500.020.9042781
TACATGA55800.020.6317312
GTATTAT57400.019.2383561
TAGTACT55700.019.0653114
TACAAGA57200.018.8945082
TATCAAC1162400.018.6964842
GTGATCG37000.018.4129818
Produced by FastQC (version 0.11.5)