##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765570_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13225286
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.666873517895947	33.0	33.0	33.0	27.0	33.0
2	31.531846343436353	33.0	33.0	33.0	27.0	33.0
3	31.546264859603035	33.0	33.0	33.0	27.0	33.0
4	35.20612348194209	37.0	37.0	37.0	33.0	37.0
5	35.37020258011812	37.0	37.0	37.0	33.0	37.0
6	35.27597822837253	37.0	37.0	37.0	33.0	37.0
7	35.212748518255104	37.0	37.0	37.0	33.0	37.0
8	35.169079519338936	37.0	37.0	37.0	33.0	37.0
9	35.05707929492035	37.0	37.0	37.0	33.0	37.0
10-11	35.10576909263058	37.0	37.0	37.0	33.0	37.0
12-13	35.065460550342735	37.0	37.0	37.0	33.0	37.0
14-15	36.56485084708187	40.0	37.0	40.0	33.0	40.0
16-17	36.67692112669624	40.0	37.0	40.0	33.0	40.0
18-19	36.72451117503243	40.0	37.0	40.0	33.0	40.0
20-21	36.85761608482417	40.0	37.0	40.0	33.0	40.0
22-23	36.89234928454477	40.0	37.0	40.0	33.0	40.0
24-25	36.91939818919606	40.0	37.0	40.0	33.0	40.0
26-27	36.899529431726464	40.0	37.0	40.0	33.0	40.0
28-29	36.85832484076337	38.5	37.0	40.0	33.0	40.0
30-31	36.72962788857647	37.0	37.0	40.0	33.0	40.0
32-33	36.56703174509799	37.0	37.0	40.0	33.0	40.0
34-35	36.4115180949584	37.0	37.0	40.0	33.0	40.0
36-37	36.27558613855307	37.0	37.0	40.0	30.0	40.0
38-39	36.1255166428915	37.0	37.0	40.0	27.0	40.0
40-41	35.970105788260454	37.0	37.0	40.0	27.0	40.0
42-43	35.770222360408695	37.0	37.0	40.0	27.0	40.0
44-45	35.54146386701959	37.0	37.0	40.0	27.0	40.0
46-47	35.2584764518514	37.0	35.0	40.0	27.0	40.0
48-49	34.91903630666286	37.0	33.0	40.0	22.0	40.0
50-51	34.764676544613096	37.0	33.0	40.0	22.0	40.0
52-53	34.58427280892073	37.0	33.0	40.0	22.0	40.0
54-55	34.43156839859645	37.0	33.0	40.0	22.0	40.0
56-57	34.27928254254766	37.0	33.0	40.0	22.0	40.0
58-59	34.1101630618801	37.0	33.0	40.0	22.0	40.0
60-61	33.90982232822791	37.0	33.0	40.0	22.0	40.0
62-63	33.66323681015291	37.0	33.0	37.0	22.0	40.0
64-65	33.45953320026501	37.0	33.0	37.0	22.0	40.0
66-67	33.26256551276093	37.0	33.0	37.0	22.0	40.0
68-69	33.05328856404315	37.0	33.0	37.0	22.0	40.0
70-71	32.82767971898679	37.0	33.0	37.0	22.0	40.0
72-73	32.57486832420864	37.0	33.0	37.0	22.0	37.0
74-75	32.324917850547806	37.0	33.0	37.0	22.0	37.0
76-77	32.112851926226774	37.0	33.0	37.0	22.0	37.0
78-79	31.92537658542885	37.0	33.0	37.0	18.5	37.0
80-81	31.731104907674585	35.0	33.0	37.0	15.0	37.0
82-83	31.55650845660351	33.0	33.0	37.0	15.0	37.0
84-85	31.38806295001862	33.0	33.0	37.0	15.0	37.0
86-87	31.243443393209038	33.0	33.0	37.0	15.0	37.0
88-89	31.097216952434906	33.0	33.0	37.0	15.0	37.0
90-91	30.94618494450706	33.0	33.0	37.0	15.0	37.0
92-93	30.798384738144794	33.0	27.0	37.0	15.0	37.0
94-95	30.65845562810513	33.0	27.0	37.0	15.0	37.0
96-97	30.55415001989371	33.0	27.0	37.0	15.0	37.0
98-99	30.429857244675084	33.0	27.0	37.0	15.0	37.0
100	30.351393459468476	33.0	27.0	37.0	15.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	15.0
6	16.0
7	266.0
8	1642.0
9	3511.0
10	5179.0
11	8098.0
12	16096.0
13	33956.0
14	60485.0
15	74205.0
16	78658.0
17	89789.0
18	104825.0
19	119880.0
20	135761.0
21	157792.0
22	176806.0
23	152292.0
24	116402.0
25	109746.0
26	119610.0
27	138219.0
28	170731.0
29	218118.0
30	282584.0
31	368492.0
32	491380.0
33	655476.0
34	866418.0
35	1187106.0
36	1946332.0
37	3516268.0
38	1816745.0
39	2387.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.81330869949758	19.254586986846032	13.321699568290263	23.610404745366125
2	15.602219020438767	21.529116930344976	38.387482273531084	24.481181775685176
3	18.10867172101291	28.20368004115989	29.053532807149384	24.634115430677817
4	12.303925379052648	17.988671596995104	36.56368045156332	33.14372257238893
5	12.87774074798039	38.91498272536754	32.876705903996736	15.330570622655337
6	29.782289774300537	38.63859730519249	17.34990078853493	14.22921213197204
7	25.626198178247332	33.48913588711805	22.179664016339608	18.705001918295
8	26.85219813015764	33.1179000590233	19.620467942999493	20.40943386781957
9	26.97035058447885	15.293597431465756	19.57793578150219	38.1581162025532
10-11	24.662744533464153	26.99412700791499	26.351944298217823	21.991184160403034
12-13	26.250725315127397	25.35567850857819	26.471488026799573	21.92210814949484
14-15	24.035631441165055	24.561846904482824	25.697247681449003	25.70527397290312
16-17	22.244413380099978	27.313309118267252	26.27090817839755	24.17136932323522
18-19	22.766173043271934	26.37017127027457	29.098328612750553	21.765327073702945
20-21	24.89381170908395	25.21497483915284	27.836305516649436	22.054907935113775
22-23	24.08491069065893	24.976505003979508	27.4003810089277	23.53820329643386
24-25	22.74428579573734	26.020520818981623	28.09259239374468	23.142600991536355
26-27	23.15228057811408	25.616602149675767	27.98036455297826	23.250752719231897
28-29	22.902836840518443	25.56439152685062	28.49430513900139	23.038466493629542
30-31	23.37791671119257	25.36273578024239	28.32067738963797	22.938670118927075
32-33	22.76854638676306	25.896472972564204	27.77564732750537	23.559333313167368
34-35	23.11959253645963	25.914190981878676	28.04708050140947	22.91913598025222
36-37	23.410439725438266	25.655075752368106	27.720746937262746	23.213737584930882
38-39	23.5191467796372	25.386810408844333	27.68967332581437	23.404369485704095
40-41	23.03422660500768	25.680917106404554	27.899378904819134	23.38547738376863
42-43	23.48295233769438	25.542298086075267	27.56239778216509	23.412351794065273
44-45	23.177508745116025	25.992727118726744	26.98091483121009	23.84884930494714
46-47	22.78214602803915	26.142193037251925	27.17484538234311	23.900815552365813
48-49	23.35816610157174	26.325045858388147	26.422099687834383	23.89468835220573
50-51	22.97877574302141	26.604041372817967	25.869112261183673	24.54807062297695
52-53	22.737942986310934	26.829226786808057	25.414323794924492	25.018506431956517
54-55	22.690382121644724	26.792173577665846	25.541707922228895	24.975736378460535
56-57	22.88497726047899	26.001920737931194	25.61511879910434	25.497983202485475
58-59	22.752478810125766	25.910891019474214	26.003648593125757	25.33298157727427
60-61	23.163450392048755	26.057415027176244	25.549328249982523	25.229806330792474
62-63	22.60983678267764	26.045318443960902	25.55185973272776	25.792985040633692
64-65	22.258040073494463	26.135389587285946	25.783585918567002	25.82298442065259
66-67	22.89969313215887	26.128142771212172	25.505956085637436	25.46620801099152
68-69	22.929184745298812	25.938465280991824	25.489401491898562	25.6429484818108
70-71	22.678353381592327	26.03881955961951	25.45563196678497	25.827195092003198
72-73	23.310301343186584	26.05069780336036	25.4986113806142	25.140389472838855
74-75	23.31953013706673	26.13002634323883	25.57364891230594	24.9767946073885
76-77	22.903089803366456	26.175798597742695	25.832472829133263	25.088638769757583
78-79	23.13320857791489	26.28494709741377	25.685440536033667	24.896403788637677
80-81	22.977867528802783	26.158949723971237	25.8482611751299	25.01492157209608
82-83	23.143498794049627	25.740167258791402	25.652611284441118	25.463722662717856
84-85	23.31345550892234	25.777524384771063	25.22342798141846	25.68559212488814
86-87	23.044316940752218	25.461463012807624	25.731973890463998	25.762246155976154
88-89	23.20253819715262	24.997132354414532	25.791003267641504	26.009326180791348
90-91	23.42357774960508	25.075979137216052	25.57552634552741	25.924916767651457
92-93	23.330378354492662	25.18694135266803	25.469516875326132	26.01316341751318
94-95	22.706218395258933	25.26544863373	25.7974158545922	26.230917116418865
96-97	23.076088126069983	25.158034733804264	25.435408838905477	26.33046830122028
98-99	22.997909837374873	25.418358610810284	25.27953113211881	26.304200419696034
100	22.842314260856654	25.572176568877826	25.28004859877024	26.30546057149529
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	647.0
1	566.0
2	762.5
3	1081.5
4	945.0
5	800.5
6	1016.5
7	1956.5
8	3168.5
9	3078.0
10	2061.5
11	1585.0
12	1741.5
13	2131.0
14	2610.5
15	3369.0
16	4260.5
17	5202.5
18	6629.5
19	8481.0
20	10617.5
21	13729.5
22	17699.0
23	22697.5
24	29598.0
25	38406.5
26	48694.5
27	59836.0
28	73075.0
29	93067.5
30	115904.5
31	136800.0
32	161293.5
33	183207.0
34	203062.0
35	219799.5
36	236378.5
37	264015.5
38	275476.0
39	272406.0
40	280130.0
41	289622.5
42	300798.0
43	330030.5
44	368437.5
45	411181.0
46	473479.0
47	544572.0
48	603903.0
49	639027.0
50	695353.0
51	703471.0
52	629916.5
53	569806.5
54	522669.0
55	480432.0
56	445191.0
57	405209.5
58	368046.5
59	329442.5
60	271586.5
61	212801.5
62	168052.0
63	133038.5
64	102061.5
65	79610.0
66	66960.0
67	55222.0
68	43667.5
69	34234.0
70	26735.5
71	21512.0
72	17899.0
73	16914.0
74	13530.5
75	9818.0
76	7816.0
77	6234.0
78	4796.5
79	3370.0
80	2509.0
81	1961.0
82	1653.0
83	1579.0
84	1243.0
85	783.0
86	571.0
87	319.5
88	130.0
89	99.0
90	93.0
91	64.0
92	48.5
93	38.5
94	24.5
95	19.5
96	15.0
97	6.5
98	4.5
99	5.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.017247264066727934
2	0.00538362648641398
3	0.0057465675978576195
4	0.003584043475505936
5	0.04462663416125746
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	3.6672174802117704E-4
18-19	0.0016937251867369825
20-21	5.70876123208224E-4
22-23	0.005001782192082652
24-25	0.007258822228872782
26-27	0.00554619385924811
28-29	0.021795369869506035
30-31	0.019999567495175532
32-33	0.012748306539457825
34-35	0.01302429300961809
36-37	0.01314905401667684
38-39	0.020589346801271443
40-41	0.029965325513565455
42-43	0.017390928256674376
44-45	0.01656674948277111
46-47	0.01773874682180786
48-49	0.012827699907586119
50-51	0.009750261733470263
52-53	0.026702636147150238
54-55	0.02519038151613508
56-57	0.01302429300961809
58-59	0.01593160253774474
60-61	0.017009083962343047
62-63	0.020419218155282238
64-65	0.010835304431223642
66-67	0.015152791402771933
68-69	0.007527247425877973
70-71	0.008721928584379953
72-73	0.010283331490903108
74-75	0.003406353556361654
76-77	0.007300409231225699
78-79	0.005621806590798868
80-81	0.002309968948875661
82-83	2.4196074096242604E-4
84-85	0.0025027814143300945
86-87	0.0023402140414959644
88-89	0.001141752246416448
90-91	8.279594104808017E-4
92-93	0.0011266297001062964
94-95	0.00310768326673616
96-97	0.0014139580799991773
98-99	0.0018033636474855815
100	0.001965931020319712
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.3225286E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.38927605152635
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.27838550541736	24.133229661990214
2	13.726506522110327	9.166362309796373
3	5.307441522040748	5.316348903202552
4	2.524923530925835	3.372214751323094
5	1.4393508738798353	2.4029441831489753
6	0.8761383969001678	1.7552176076064878
7	0.616175283803387	1.4401552652927216
8	0.43762884291898574	1.1689688195465664
9	0.33456427912244313	1.005377316533925
>10	2.0291315555435596	12.876938776819088
>50	0.20948940940912966	4.875963196847464
>100	0.1751452174554622	12.142712418083786
>500	0.026810137720466077	6.270206165308852
>1k	0.017215855217033654	10.247689362175631
>5k	7.97028411100787E-4	1.7701980321194017
>10k+	2.960391241231494E-4	2.055473230204946
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	45076	0.3408319487381974	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	44284	0.33484342039937737	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34310	0.2594272819506512	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21572	0.16311178450129546	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	17612	0.13316914280719525	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	17191	0.12998584680890832	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15560	0.11765341029297968	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	14964	0.11314689149255448	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	13872	0.10488998120721171	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	13688	0.10349870694667775	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	7.561273155075815E-6	0.0	7.561273155075815E-6	0.0
5	7.561273155075815E-6	3.024509262030326E-5	0.0	7.561273155075815E-6	0.0
6	1.512254631015163E-5	3.024509262030326E-5	0.0	7.561273155075815E-6	0.0
7	3.024509262030326E-5	3.024509262030326E-5	0.0	7.561273155075815E-6	0.0
8	5.2928912085530704E-5	3.024509262030326E-5	0.0	7.561273155075815E-6	0.0
9	5.2928912085530704E-5	4.536763893045489E-5	0.0	2.2683819465227445E-5	0.0
10-11	5.670954866306861E-5	4.536763893045489E-5	0.0	3.780636577537907E-5	0.0
12-13	1.058578241710614E-4	4.536763893045489E-5	0.0	5.2928912085530704E-5	0.0
14-15	1.6256737283413001E-4	4.536763893045489E-5	0.0	7.561273155075814E-5	0.0
16-17	1.8525119229935747E-4	4.536763893045489E-5	0.0	9.82965510159856E-5	0.0
18-19	1.9659310203197118E-4	6.427082181814443E-5	0.0	1.1719973390367512E-4	0.0
20-21	2.570832872725777E-4	7.561273155075814E-5	0.0	2.6842519700519144E-4	0.0
22-23	2.9867028962549466E-4	7.561273155075814E-5	0.0	0.0010850426977533794	0.0
24-25	4.196506601067077E-4	7.561273155075814E-5	0.0	0.00410199068662863	0.0
26-27	7.636885886626572E-4	7.561273155075814E-5	0.0	0.007810795169193316	0.0
28-29	8.090562275931122E-4	7.561273155075814E-5	0.0	0.02731887990928892	0.0
30-31	8.317400470583396E-4	7.561273155075814E-5	0.0	0.11022445941811769	0.0
32-33	8.619851396786429E-4	7.561273155075814E-5	0.0	0.25028948334274204	0.0
34-35	8.695464128337187E-4	8.317400470583396E-5	0.0	0.42137084974948746	0.0
36-37	8.733270494112567E-4	9.451591443844769E-5	0.0	0.7040301434691091	0.0
38-39	8.771076859887945E-4	9.829655101598559E-5	0.0	1.196994908087432	0.0
40-41	8.884495957214082E-4	9.829655101598559E-5	0.0	1.830924488135833	0.0
42-43	9.224753249192493E-4	9.829655101598559E-5	0.0	2.3515483899554233	0.0
44-45	9.37597871229401E-4	1.096384607485993E-4	0.0	2.895003556066765	0.0
46-47	9.451591443844768E-4	1.1341909732613721E-4	0.0	3.4446816499847337	0.0
48-49	9.451591443844768E-4	1.1341909732613721E-4	0.0	4.061484946336888	0.0
50-51	9.640623272721663E-4	1.4366418994644047E-4	0.0	4.730831529843664	0.0
52-53	0.0010132106027801592	1.5122546310151629E-4	0.0	5.330769406423423	0.0
54-55	0.0010245525125127729	1.587867362565921E-4	0.0	5.8675366264291	0.0
56-57	0.0010358944222453865	1.587867362565921E-4	0.0	6.469209815197948	0.0
58-59	0.0010434556954004625	1.6634800941166792E-4	0.0	7.128772111242055	0.0
60-61	0.0010547976051330761	1.6634800941166792E-4	0.0	7.829301385240364	0.0
62-63	0.001062358878288152	1.7012864598920583E-4	0.0	8.562612559002506	0.0
64-65	0.0010661395148656898	1.8903182887689536E-4	0.0	9.217929200170037	0.0
66-67	0.0010661395148656898	2.079350117645849E-4	0.0	9.87378269173158	0.0
68-69	0.0010812620611758417	2.1171564834212282E-4	0.0	10.62693464625264	0.0
70-71	0.0010926039709084553	2.1171564834212282E-4	0.0	11.459128369700284	0.0
72-73	0.001103945880641069	2.1171564834212282E-4	0.0	12.25701659684335	0.0
74-75	0.0011077265172186068	2.1171564834212282E-4	0.0	12.996452401861102	0.0
76-77	0.0011266297001062964	2.3061883122981234E-4	0.0	13.746402157200986	0.0
78-79	0.0011606554293041374	2.4196074096242607E-4	0.0	14.515527301262143	0.0
80-81	0.0011644360658816754	2.495220141175019E-4	0.0	15.358208510575878	0.0
82-83	0.0011757779756142893	2.533026506950398E-4	0.0	16.252518092992467	0.0
84-85	0.001187119885346903	2.570832872725777E-4	0.0	17.077101395009528	0.0
86-87	0.001187119885346903	2.6842519700519144E-4	0.0	17.874993402789173	0.0
88	0.001187119885346903	2.7220583358272935E-4	0.0	18.48546035223737	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACAAG	6730	0.0	38.472336	1
GTACATA	4165	0.0	31.703217	1
GTACAAA	6225	0.0	28.081196	1
GTACTAG	4790	0.0	24.91783	1
AATACGG	9120	0.0	24.575983	5
TACAAGG	6960	0.0	24.237564	2
TACATAG	3640	0.0	24.011282	2
GTATCAA	98910	0.0	22.029716	1
GTAACAA	4645	0.0	21.952602	1
TAATACG	10990	0.0	21.93362	4
CAACACA	18895	0.0	21.311857	5
TTAATAC	12585	0.0	21.2453	3
ACGGACC	11335	0.0	21.05717	8
GTATAAG	5350	0.0	20.904278	1
TACATGA	5580	0.0	20.631731	2
GTATTAT	5740	0.0	19.238356	1
TAGTACT	5570	0.0	19.065311	4
TACAAGA	5720	0.0	18.894508	2
TATCAAC	116240	0.0	18.696484	2
GTGATCG	3700	0.0	18.412981	8
>>END_MODULE