FastQCFastQC Report
Fri 27 May 2016
ERR765569_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765569_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13040720
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA759710.5825675269463649No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT658780.505171493598513No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG309980.23770159929819829No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG299760.22986460870258696No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG259450.1989537387506211No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC235800.18081823702985725No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC233010.1786787846069849No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229270.17581084479998038No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT179690.1377914716365354No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC177730.13628848713874694No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT165540.12694084375709316No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC157040.12042279874117381No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA153820.11795360992337847No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148230.11366703678937973No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAG58500.023.1180321
GTACATA43750.019.1054041
CATGGGG424950.018.4534634
GGGGATA86850.017.4635267
GTATAAG46400.017.4070321
AATACGG51950.016.6315045
TAGTACT52050.016.599554
TACATGA53750.016.5987112
TAATACG53700.016.439284
GTACTAG42450.016.2612481
ATGGGAG149050.016.0986025
CTAACGC20600.015.9562223
CCTACAC48450.015.894593
GTACATG1873850.015.8904851
TACATGG1828900.015.679682
TAGACGA50800.015.6214825
TCTAACG22350.015.3371462
TACACTA41100.015.3095445
GTATAAA113100.015.15461
GTATTAG39400.015.1363711
Produced by FastQC (version 0.11.5)