##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765567_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7936907 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.198347038714196 33.0 31.0 34.0 31.0 34.0 2 32.24726508701689 34.0 31.0 34.0 31.0 34.0 3 32.27760171563053 34.0 31.0 34.0 30.0 34.0 4 35.745523287598054 37.0 35.0 37.0 35.0 37.0 5 35.75810841679259 37.0 35.0 37.0 35.0 37.0 6 35.8453209795705 37.0 37.0 37.0 35.0 37.0 7 35.79521329908489 37.0 37.0 37.0 35.0 37.0 8 35.824351727946414 37.0 37.0 37.0 35.0 37.0 9 37.52297979552992 39.0 38.0 39.0 35.0 39.0 10-11 37.44311417533304 39.0 38.0 39.0 35.0 39.0 12-13 37.41928367057848 39.0 38.0 39.0 35.0 39.0 14-15 38.80115030956013 41.0 38.5 41.0 35.0 41.0 16-17 38.7587220059401 40.5 38.5 41.0 35.0 41.0 18-19 38.74913483300233 41.0 38.5 41.0 35.0 41.0 20-21 38.72487135101873 41.0 39.0 41.0 35.0 41.0 22-23 38.625644032366765 40.0 38.5 41.0 34.5 41.0 24-25 38.54476227326337 40.0 38.0 41.0 34.0 41.0 26-27 38.42015113696053 40.0 38.0 41.0 34.0 41.0 28-29 38.27854175436351 40.0 38.0 41.0 34.0 41.0 30-31 38.056563028897784 40.0 38.0 41.0 34.0 41.0 32-33 37.823458117878914 40.0 37.5 41.0 33.0 41.0 34-35 37.65463139482421 40.0 37.0 41.0 33.0 41.0 36-37 37.48695959269776 40.0 37.0 41.0 33.0 41.0 38-39 37.28533470280047 40.0 37.0 41.0 32.0 41.0 40-41 37.03787445159682 40.0 36.5 41.0 31.0 41.0 42-43 36.763821725515996 40.0 36.0 41.0 31.0 41.0 44-45 36.398925866209595 40.0 35.5 41.0 30.0 41.0 46-47 35.9654342050373 39.0 35.0 41.0 29.5 41.0 48-49 35.569992252649556 39.0 35.0 41.0 27.5 41.0 50-51 34.27978865066707 38.0 34.0 40.0 25.5 40.5 52-53 34.207896980524026 38.0 33.5 39.5 25.0 40.0 54-55 35.0287688894427 39.0 35.0 40.5 26.0 41.0 56-57 35.03991964376047 39.0 35.0 41.0 26.0 41.0 58-59 34.81430896191678 38.0 35.0 41.0 26.0 41.0 60-61 34.473412816856744 37.5 35.0 40.0 26.0 41.0 62-63 34.11321337140526 37.0 34.0 40.0 25.0 41.0 64-65 33.7817991945729 36.5 34.0 39.5 24.5 41.0 66-67 33.46143894592692 36.0 34.0 39.0 23.0 41.0 68-69 33.205902563807285 35.5 34.0 39.0 23.0 41.0 70-71 32.86687320388157 35.0 34.0 38.5 22.5 40.5 72-73 32.50790282663007 35.0 34.0 37.0 21.5 39.5 74-75 32.15969180437669 35.0 34.0 37.0 20.0 39.0 76-77 31.83100967417156 35.0 33.0 36.0 19.5 39.0 78-79 31.522668150200072 35.0 33.0 36.0 18.0 37.5 80-81 31.230372675401135 35.0 33.0 35.0 16.0 37.0 82-83 31.000144136752517 35.0 33.0 35.0 11.0 36.5 84-85 30.772913428366994 35.0 33.0 35.0 7.0 36.0 86-87 30.55302739467654 35.0 33.0 35.0 2.0 36.0 88-89 30.349272014904546 35.0 32.5 35.0 2.0 36.0 90-91 30.121881042577417 35.0 32.0 35.0 2.0 35.0 92-93 29.8642326916518 35.0 32.0 35.0 2.0 35.0 94-95 29.554040635728754 35.0 31.5 35.0 2.0 35.0 96-97 29.258436189311528 35.0 31.0 35.0 2.0 35.0 98-99 28.960061520186642 35.0 31.0 35.0 2.0 35.0 100 28.412063414627386 34.0 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4516302433688395 1101 2 0.5778723935998755 1101 3 0.5856663100681629 1101 4 0.49204332403403583 1101 5 0.5359345170429535 1101 6 0.4985715103732602 1101 7 0.4835350429137435 1101 8 0.46988887208552654 1101 9 0.5659853836360327 1101 10-11 0.5185984112958266 1101 12-13 0.5927295895788376 1101 14-15 0.7435360824139892 1101 16-17 0.743516594115107 1101 18-19 0.6767189121238388 1101 20-21 0.6882397440250472 1101 22-23 0.7661275859822965 1101 24-25 0.6661596710301936 1101 26-27 0.7709710217144305 1101 28-29 0.7826353071492278 1101 30-31 0.7491236945111552 1101 32-33 0.6834607316062957 1101 34-35 0.7846455464892088 1101 36-37 0.7595523599105078 1101 38-39 0.7901440833235611 1101 40-41 0.8311304433457778 1101 42-43 0.8437686531195538 1101 44-45 0.8671767917270046 1101 46-47 0.941588725270293 1101 48-49 0.9225437150086648 1101 50-51 0.8034783818740223 1101 52-53 0.9390182537704987 1101 54-55 1.0156004069078293 1101 56-57 1.086756214589407 1101 58-59 1.0388465738871773 1101 60-61 1.0345678303657806 1101 62-63 1.0126282191629699 1101 64-65 0.9837646760824583 1101 66-67 0.9702942572466711 1101 68-69 0.8764517630076085 1101 70-71 0.8321389536281245 1101 72-73 0.777461377660245 1101 74-75 0.7734978520381048 1101 76-77 0.7845215953961073 1101 78-79 0.7446531872495861 1101 80-81 0.7676051318922141 1101 82-83 0.818929972322568 1101 84-85 0.8196448772306546 1101 86-87 0.7639278827890976 1101 88-89 0.732585215190916 1101 90-91 0.7147895794180954 1101 92-93 0.707366954693768 1101 94-95 0.5834612729020527 1101 96-97 0.49987184357173575 1101 98-99 0.5576584689580493 1101 100 0.6823859723768351 1102 1 0.5408472635233323 1102 2 0.6058160184242993 1102 3 0.6590242238143631 1102 4 0.5350168148700263 1102 5 0.5651702666063514 1102 6 0.5421270920119596 1102 7 0.5578829093531184 1102 8 0.5189052461776527 1102 9 0.48661852422703333 1102 10-11 0.5682861681762574 1102 12-13 0.6001541418342953 1102 14-15 0.8147138377289878 1102 16-17 0.8313731761825451 1102 18-19 0.8022357733054051 1102 20-21 0.821612640236161 1102 22-23 0.8864327959472504 1102 24-25 0.8456117329182362 1102 26-27 0.8195462336484596 1102 28-29 0.8792138772585716 1102 30-31 0.8420978956997445 1102 32-33 0.8856380486270936 1102 34-35 0.9144715658684248 1102 36-37 0.87144892035667 1102 38-39 0.8841239047953522 1102 40-41 0.8654252842872054 1102 42-43 0.834521907032368 1102 44-45 0.8969201579299693 1102 46-47 0.7739285298051612 1102 48-49 0.8485562296953688 1102 50-51 0.7642919998246143 1102 52-53 0.752896671412941 1102 54-55 0.7992491124528698 1102 56-57 0.7897918234366017 1102 58-59 0.8771820730214159 1102 60-61 0.7658999034760683 1102 62-63 0.7534048350458491 1102 64-65 0.8549466802354289 1102 66-67 0.809735967903201 1102 68-69 0.7697586083768613 1102 70-71 0.7245564242656712 1102 72-73 0.7367807324051583 1102 74-75 0.7208448178566016 1102 76-77 0.658412844529245 1102 78-79 0.5305152187172801 1102 80-81 0.6148463016451053 1102 82-83 0.6378150613045186 1102 84-85 0.5986259554290569 1102 86-87 0.5274470147090078 1102 88-89 0.5398544060302655 1102 90-91 0.5949624301135295 1102 92-93 0.6882915044928701 1102 94-95 0.6145649709711272 1102 96-97 0.5856322481460872 1102 98-99 0.5742445249888029 1102 100 0.619341847265023 1103 1 0.5363049011136312 1103 2 0.5560011176164608 1103 3 0.6057610986873669 1103 4 0.4997107518071289 1103 5 0.5402791270129086 1103 6 0.5199782012325898 1103 7 0.551189478249448 1103 8 0.52383262303767 1103 9 0.5891099806963922 1103 10-11 0.5737886622305552 1103 12-13 0.5522764100493163 1103 14-15 0.6979319670808053 1103 16-17 0.7290733345535685 1103 18-19 0.7060570342882144 1103 20-21 0.7264474891900008 1103 22-23 0.7058046621739251 1103 24-25 0.7210985381046697 1103 26-27 0.7554888719488275 1103 28-29 0.6789451727684934 1103 30-31 0.7381497574337388 1103 32-33 0.814118379586823 1103 34-35 0.7717646411138332 1103 36-37 0.7072433806452167 1103 38-39 0.777249289837691 1103 40-41 0.8482127933693917 1103 42-43 0.8012884695635165 1103 44-45 0.793751840008575 1103 46-47 0.7720949889634454 1103 48-49 0.7150220412078951 1103 50-51 0.7701815657718853 1103 52-53 0.7500325996838484 1103 54-55 0.8108845456113514 1103 56-57 0.8307823402867669 1103 58-59 0.8587638587343989 1103 60-61 0.8311678966611211 1103 62-63 0.8312523177139397 1103 64-65 0.8350314905006755 1103 66-67 0.8245049110194458 1103 68-69 0.804516437336595 1103 70-71 0.8001702058891524 1103 72-73 0.692619027129254 1103 74-75 0.7870258941782353 1103 76-77 0.7412091088975359 1103 78-79 0.7195723728135128 1103 80-81 0.687483211626013 1103 82-83 0.5727584765497689 1103 84-85 0.6492656800411005 1103 86-87 0.6582522533942985 1103 88-89 0.7080750946672296 1103 90-91 0.6577104030801202 1103 92-93 0.6340985516206779 1103 94-95 0.5234587204925596 1103 96-97 0.6215283262711999 1103 98-99 0.6368482290846238 1103 100 0.7309456924815763 1104 1 0.42280972906027614 1104 2 0.5444229841935595 1104 3 0.518945355439385 1104 4 0.46559782404953154 1104 5 0.46124882245786125 1104 6 0.41752689457022285 1104 7 0.42586647276669964 1104 8 0.4336154230548175 1104 9 0.5458731322290831 1104 10-11 0.5576388127529697 1104 12-13 0.5498118448170146 1104 14-15 0.6285290347332051 1104 16-17 0.6413372844927423 1104 18-19 0.6520219919062384 1104 20-21 0.633102301954473 1104 22-23 0.6446548567691437 1104 24-25 0.6760015875665673 1104 26-27 0.6853757705627288 1104 28-29 0.6873771962916209 1104 30-31 0.708035174558546 1104 32-33 0.6624837007504354 1104 34-35 0.6048206638972715 1104 36-37 0.6454097351141854 1104 38-39 0.7181598193734118 1104 40-41 0.7317852186290281 1104 42-43 0.7738730822401934 1104 44-45 0.8071623348828609 1104 46-47 0.7996436391464599 1104 48-49 0.7872368876109377 1104 50-51 0.743805206389716 1104 52-53 0.7831754780367532 1104 54-55 0.82932960137218 1104 56-57 0.8226724844039381 1104 58-59 0.8839381322607167 1104 60-61 0.813611676973224 1104 62-63 0.7999155407639407 1104 64-65 0.8497985556069523 1104 66-67 0.8612756357297613 1104 68-69 0.7361267090188264 1104 70-71 0.8088064334256799 1104 72-73 0.8141912414821348 1104 74-75 0.7941137199922039 1104 76-77 0.7643267316947089 1104 78-79 0.8078354487337869 1104 80-81 0.8068081144431751 1104 82-83 0.7862421231045751 1104 84-85 0.8053631554128948 1104 86-87 0.7521372062855143 1104 88-89 0.7316103188036287 1104 90-91 0.6897928253841208 1104 92-93 0.657785758083147 1104 94-95 0.6719938528106049 1104 96-97 0.6090799729461054 1104 98-99 0.5837508471839143 1104 100 0.665441906765043 1105 1 0.31011943856892543 1105 2 0.31934059661060843 1105 3 0.35362490383988643 1105 4 0.3453142323869116 1105 5 0.32790261921620356 1105 6 0.3194997619962976 1105 7 0.36776074312660967 1105 8 0.3371006843020794 1105 9 0.28349004517848897 1105 10-11 0.4132339860565821 1105 12-13 0.40779316626427686 1105 14-15 0.4907593945430193 1105 16-17 0.5255368513476597 1105 18-19 0.5214510595087347 1105 20-21 0.4923976015312874 1105 22-23 0.48381737718678863 1105 24-25 0.5030175448714118 1105 26-27 0.4155351339017912 1105 28-29 0.4563202894693674 1105 30-31 0.4740337995947854 1105 32-33 0.44152359343230785 1105 34-35 0.45625754163872045 1105 36-37 0.4506833941805297 1105 38-39 0.45539407496912077 1105 40-41 0.45227347996299017 1105 42-43 0.36441543614698446 1105 44-45 0.3614454489387029 1105 46-47 0.258788035041853 1105 48-49 0.36109313460310233 1105 50-51 0.31309076401510794 1105 52-53 0.29616674285585987 1105 54-55 0.35176275921831746 1105 56-57 0.32588998911494116 1105 58-59 0.37306946981332345 1105 60-61 0.37133582681742183 1105 62-63 0.3687205904982065 1105 64-65 0.4121833294499524 1105 66-67 0.34987706351042647 1105 68-69 0.32320316041873554 1105 70-71 0.2912739877457824 1105 72-73 0.2821134439638655 1105 74-75 0.3006314526978642 1105 76-77 0.31344133974798183 1105 78-79 0.24897144995656717 1105 80-81 0.28005865376085737 1105 82-83 0.24607779305292965 1105 84-85 0.2249958068400204 1105 86-87 0.21765548091984854 1105 88-89 0.23499310430381115 1105 90-91 0.2543707194681488 1105 92-93 0.25449110023344446 1105 94-95 0.23497911646164837 1105 96-97 0.20781665661110793 1105 98-99 0.2724920590783597 1105 100 0.3131081486528977 1106 1 0.33174958947962097 1106 2 0.34455917314291895 1106 3 0.3627043747231582 1106 4 0.4011085391673035 1106 5 0.40285004553931003 1106 6 0.3808597080589706 1106 7 0.3875784481817064 1106 8 0.3968551970798089 1106 9 0.44585165485778333 1106 10-11 0.4795231710982222 1106 12-13 0.512738360809827 1106 14-15 0.5740652886074997 1106 16-17 0.5486560783133925 1106 18-19 0.5825764876359187 1106 20-21 0.5511939210660941 1106 22-23 0.547260800341526 1106 24-25 0.6024509305791881 1106 26-27 0.5455422953054594 1106 28-29 0.5731676776192245 1106 30-31 0.5414470409890058 1106 32-33 0.5262736277361171 1106 34-35 0.5687642848437662 1106 36-37 0.6317575748936335 1106 38-39 0.62191794601884 1106 40-41 0.6436612471403578 1106 42-43 0.6664296012304618 1106 44-45 0.7408240943437718 1106 46-47 0.7248345647901573 1106 48-49 0.6698965075218766 1106 50-51 0.6282117405977559 1106 52-53 0.6898121745499637 1106 54-55 0.7562966328918534 1106 56-57 0.8101932732196175 1106 58-59 0.7664340786254726 1106 60-61 0.7480393229126392 1106 62-63 0.7522532636045867 1106 64-65 0.747755814763714 1106 66-67 0.7884595573022182 1106 68-69 0.739645893947035 1106 70-71 0.6795557599924464 1106 72-73 0.6064558089146033 1106 74-75 0.7028585393315083 1106 76-77 0.6418330741564091 1106 78-79 0.6739221508627615 1106 80-81 0.7126481034167504 1106 82-83 0.6853242019551438 1106 84-85 0.6736476813802064 1106 86-87 0.642593869091499 1106 88-89 0.641660055371986 1106 90-91 0.5844051747295431 1106 92-93 0.6348035400270966 1106 94-95 0.5379246114983509 1106 96-97 0.57835923425489 1106 98-99 0.5871229812723406 1106 100 0.6195438223764604 1107 1 0.24368481006686693 1107 2 0.2599266319391731 1107 3 0.3607417727767199 1107 4 0.3335661779107042 1107 5 0.3419203445580621 1107 6 0.29645722805128827 1107 7 0.34426412108030036 1107 8 0.3256625385413372 1107 9 0.3217334989982419 1107 10-11 0.3809458848580505 1107 12-13 0.35588730462825424 1107 14-15 0.389974412185758 1107 16-17 0.4765504940013514 1107 18-19 0.4215992666816817 1107 20-21 0.36690150386694853 1107 22-23 0.3798812286440594 1107 24-25 0.43086807651365433 1107 26-27 0.4070893517645402 1107 28-29 0.46342240695658177 1107 30-31 0.40089854958208804 1107 32-33 0.39541736041892506 1107 34-35 0.37914607044971405 1107 36-37 0.4217234765628888 1107 38-39 0.33046353505580583 1107 40-41 0.36865867186779866 1107 42-43 0.42213849987169993 1107 44-45 0.3761477698163418 1107 46-47 0.35632731366261794 1107 48-49 0.3819524540045691 1107 50-51 0.4351083415859236 1107 52-53 0.4627454994343623 1107 54-55 0.47792934233619633 1107 56-57 0.39917980866494673 1107 58-59 0.36296203308165076 1107 60-61 0.3549093821630649 1107 62-63 0.36374268175606517 1107 64-65 0.3718263135835542 1107 66-67 0.37022700894288363 1107 68-69 0.41770338387113526 1107 70-71 0.3664816240196771 1107 72-73 0.38342037109067917 1107 74-75 0.4062974973236848 1107 76-77 0.44741349208966597 1107 78-79 0.416376031166763 1107 80-81 0.43309776023861346 1107 82-83 0.4168422413923274 1107 84-85 0.43273072303382776 1107 86-87 0.4211908518174745 1107 88-89 0.4272742736037607 1107 90-91 0.42257008312909505 1107 92-93 0.3332565296083807 1107 94-95 0.3692171797168875 1107 96-97 0.3790729054534374 1107 98-99 0.37894685730161726 1107 100 0.36961851494550046 1108 1 0.1889364182433937 1108 2 0.2665769974374541 1108 3 0.31971400190035837 1108 4 0.2766126188914342 1108 5 0.3107115157576814 1108 6 0.26479824755812587 1108 7 0.3506794779940421 1108 8 0.3345889909857718 1108 9 0.352797210494316 1108 10-11 0.35804716554557814 1108 12-13 0.387325877661965 1108 14-15 0.4463614409303034 1108 16-17 0.4381149194626417 1108 18-19 0.4548271231650105 1108 20-21 0.41641814040872305 1108 22-23 0.37925324782933956 1108 24-25 0.4330724490903961 1108 26-27 0.41007130874704245 1108 28-29 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-1.1713394127826149 2316 78-79 -1.100461885035969 2316 80-81 -1.03160725260555 2316 82-83 -0.9629209183585559 2316 84-85 -1.023276907058726 2316 86-87 -1.0536030874075344 2316 88-89 -1.0320614945198194 2316 90-91 -0.9637198136059659 2316 92-93 -0.9131838570927542 2316 94-95 -0.741088146607467 2316 96-97 -0.9402874716172107 2316 98-99 -0.7166759738494406 2316 100 -0.8933179397802746 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 23688.0 3 3709.0 4 3634.0 5 4287.0 6 4864.0 7 6205.0 8 7496.0 9 9286.0 10 11838.0 11 16912.0 12 25291.0 13 34957.0 14 37444.0 15 46082.0 16 60796.0 17 82962.0 18 81214.0 19 47728.0 20 41225.0 21 38898.0 22 40687.0 23 46755.0 24 51851.0 25 55740.0 26 62057.0 27 70879.0 28 82985.0 29 98655.0 30 119643.0 31 147642.0 32 190020.0 33 255785.0 34 372123.0 35 606517.0 36 1121862.0 37 1969504.0 38 1701221.0 39 354116.0 40 349.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.08408981743625 18.23414308983976 13.546083308732651 24.135683783991343 2 15.448107575233397 21.596949700021657 37.0963572235255 25.85858550121945 3 18.454595055353145 26.6654148914582 29.64274874672957 25.237241306459087 4 13.343578419708274 17.9292255010402 35.13452583577676 33.59267024347476 5 14.01195452185199 36.32394408212237 33.32028451397972 16.34381688204592 6 30.99552602494466 35.01980657494738 17.9805484503518 16.00411894975616 7 25.977073884399964 32.08109851463714 22.145022008414575 19.79680559254832 8 27.16931404543822 31.102311332147337 21.14964943756819 20.578725184846252 9 27.444718483901774 14.510134655287892 19.711347442963564 38.33379941784676 10-11 24.99885717489593 25.19717837113201 26.633943143125144 23.170021310846913 12-13 26.75188103440916 23.436986515559155 26.788836126772892 23.022296323258796 14-15 24.944145146075154 23.233101386475077 25.286666893420072 26.5360865740297 16-17 23.055989263327064 26.313120780945308 26.583266476259404 24.04762347946823 18-19 23.851174929782644 25.428394328894004 28.493661221732097 22.22676951959125 20-21 24.540307968441322 24.740469400558585 27.689509450142207 23.029713180857883 22-23 23.659754438740784 25.209567631576707 26.568904093034735 24.561773836647777 24-25 23.344225076442715 25.94609262555813 26.324774585354838 24.384907712644317 26-27 23.86073241601545 25.186812931558922 26.601804195127976 24.350650457297657 28-29 23.288536745829404 24.630350808737827 28.33681856466556 23.744293880767206 30-31 24.271797566595254 24.698995007882292 27.61128683455273 23.417920590969725 32-33 22.630913584412596 25.591416845784398 27.40114260718051 24.376526962622496 34-35 22.769253560464087 25.856811446180583 27.77647490158868 23.59746009176665 36-37 23.606815589124523 25.79948652678536 26.92143950664983 23.67225837744029 38-39 23.903383643656817 24.89839116746898 26.82910246745342 24.369122721420787 40-41 23.35159897519684 25.47420731692705 26.940081202772713 24.234112505103393 42-43 24.47873527012745 25.140370100228854 26.63020429005843 23.750690339585265 44-45 24.051525415688406 25.10962874534653 26.948503728645314 23.890342110319757 46-47 23.426250568744482 25.197065859222047 27.62992876112429 23.746754810909188 48-49 23.815384129789784 25.910636954737704 26.875216211097786 23.39876270437472 50-51 23.116918175219244 26.234458824478608 25.922382158909162 24.726240841392983 52-53 22.937876687798855 26.235648903393237 25.310158559867784 25.516315848940124 54-55 23.48235095321908 25.71462348689692 25.36436319707091 25.43866236281309 56-57 23.836717650876686 24.884530998145294 25.67529032426942 25.6034610267086 58-59 23.621001686382556 24.754595725252692 26.827525856425382 24.796876731939363 60-61 23.51555546248847 25.123950220098052 26.325306129039422 25.035188188374057 62-63 22.401428513750172 25.286346224696306 26.635674739960535 25.67655052159299 64-65 22.530353566353263 25.156879632944296 26.626788941203124 25.68597785949932 66-67 23.453988925730236 25.036951024833538 26.03366053244544 25.475399516990787 68-69 23.461985288859285 24.395352794061058 26.15559290861417 25.987069008465486 70-71 22.828030753854986 24.64259045327533 26.44223193668974 26.087146856179942 72-73 23.593061574988656 24.329745544392658 26.497523838847936 25.579669041770746 74-75 23.594566772662784 24.053601352720783 26.743704945890094 25.60812692872634 76-77 22.909864921482026 24.653907326318492 27.07279013527415 25.36343761692533 78-79 23.015025316357416 24.484733081014554 27.093391298606395 25.40685030402164 80-81 22.658293647973384 24.439608580021382 26.904066437445177 25.998031334560057 82-83 22.807368731443574 24.75591096019438 26.123492190828856 26.31322811753319 84-85 22.967227566322443 24.56906716893334 26.080840053891098 26.38286521085311 86-87 23.005815633401134 23.83820822292769 26.616517226940985 26.53945891673019 88-89 23.170307524237046 23.294067401300047 27.055434984542003 26.480190089920903 90-91 23.512183048671044 23.63996665219643 26.584107440458894 26.263742858673634 92-93 23.11250096425109 23.76900536530436 26.75418797191142 26.364305698533137 94-95 22.784109359557075 23.807629140158156 27.059653127557485 26.348608372727284 96-97 23.563672455345678 23.545817428302012 26.430479138133293 26.460030978219017 98-99 23.57771831597269 23.21161304614298 26.411196114882806 26.799472523001526 100 23.560868699145736 23.29102960282449 26.63883399947303 26.509267698556748 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2688.0 1 3643.5 2 2915.5 3 1084.0 4 837.0 5 896.5 6 1214.0 7 1885.0 8 2880.0 9 3128.0 10 2530.5 11 2230.0 12 2528.5 13 2897.5 14 3188.5 15 3683.5 16 4252.0 17 4793.5 18 5517.5 19 6366.0 20 7442.0 21 8825.0 22 10729.5 23 13176.5 24 15898.5 25 19472.0 26 24688.5 27 30980.5 28 37453.5 29 46127.5 30 56116.0 31 65200.0 32 74865.5 33 84851.5 34 94988.0 35 103237.5 36 110263.0 37 120131.0 38 129653.5 39 138631.5 40 145723.0 41 152963.0 42 163297.0 43 182464.5 44 206971.5 45 230202.5 46 261670.5 47 299960.0 48 388567.5 49 474120.5 50 521526.5 51 489838.5 52 396494.5 53 352843.0 54 320576.5 55 288778.5 56 267232.5 57 242053.5 58 222657.5 59 203095.0 60 174584.5 61 145707.0 62 119118.5 63 93892.0 64 70074.0 65 55214.0 66 43332.0 67 32631.0 68 25887.5 69 20891.0 70 17382.5 71 14543.5 72 12059.0 73 11674.5 74 9697.0 75 7552.0 76 5895.0 77 4129.0 78 2976.5 79 2074.0 80 1505.5 81 1197.0 82 895.0 83 646.5 84 466.5 85 305.0 86 207.0 87 170.5 88 136.0 89 88.5 90 68.5 91 67.5 92 54.5 93 46.0 94 41.0 95 28.5 96 26.5 97 23.0 98 18.5 99 12.0 100 5.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08706162236750412 2 0.28561503870462385 3 0.28742934747755017 4 0.296337099577959 5 0.32243038755525294 6 0.17044422972324105 7 0.18819673709166554 8 0.1538760628038101 9 0.20552086599981578 10-11 0.2259696377946724 12-13 0.24176924335890543 14-15 0.1696945674177611 16-17 0.15956467676892272 18-19 0.17649192563299532 20-21 0.20851951522173562 22-23 0.1717230654208245 24-25 0.19675170693067212 26-27 0.19503819308957507 28-29 0.22397893789104498 30-31 0.25736725905947994 32-33 0.226788596615785 34-35 0.1528618138022784 36-37 0.14695901060702868 38-39 0.11437074920998822 40-41 0.10533070376155347 42-43 0.1215712871525394 44-45 0.13655193389565987 46-47 0.2009724947010214 48-49 0.20671780581528798 50-51 0.21290409475630745 52-53 0.14064042831798332 54-55 0.08573238920400604 56-57 0.12321550447800383 58-59 0.14316030161371426 60-61 0.12008456190805814 62-63 0.14094911279671035 64-65 0.11557398870869974 66-67 0.11997116760975024 68-69 0.10530550502859616 70-71 0.13047903925294826 72-73 0.15550138107955655 74-75 0.19391054978973546 76-77 0.17448232667964989 78-79 0.19960546343808738 80-81 0.1031321143110282 82-83 0.1438595664532796 84-85 0.17502409943823205 86-87 0.18878890731616232 88-89 0.17007884809536006 90-91 0.1731971912988271 92-93 0.12310211017969594 94-95 0.3622947830937165 96-97 0.36248377359089634 98-99 0.4093093191088166 100 0.4430063247559787 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7936907.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.37248851045355 #Duplication Level Percentage of deduplicated Percentage of total 1 76.57359675550404 30.9146665521569 2 12.690795090512323 10.247179579604579 3 4.076014658812993 4.936765649441035 4 1.8671260294613883 3.015220966879946 5 1.0263062287956028 2.0717268215128692 6 0.7104638555929035 1.7209916308210214 7 0.49358564896791574 1.3949096659317355 8 0.3437560136977246 1.1102628570728559 9 0.26013534311237774 0.9452080035870626 >10 1.666210549389917 12.70852733411069 >50 0.1517123620946501 4.231420821972199 >100 0.11584783727497618 9.607917642914282 >500 0.015014728271603491 4.182886396840284 >1k 0.008337817290678824 6.11779957001986 >5k 5.642131992866803E-4 1.595368891718444 >10k+ 5.328680215485313E-4 5.19914761541613 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 54357 0.6848637636802346 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 53496 0.674015709142113 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 32486 0.40930301942557723 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 31219 0.39333962209712175 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 30335 0.3822017821299909 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24966 0.3145557835060937 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 23617 0.29755923812638857 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 20473 0.25794682991749807 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 19299 0.24315517367155745 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 18317 0.23078259578951849 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 17216 0.21691069329651966 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16836 0.21212293403463084 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 16832 0.21207253656871625 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 14256 0.1796165685197017 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 13207 0.16639983308359288 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12038 0.1516711736700455 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 11893 0.1498442655306406 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 9957 0.12545189202796506 No Hit CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC 9597 0.12091612009564935 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC 8997 0.11335650020845651 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 8990 0.11326830464310593 No Hit GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG 7994 0.10071933563036584 No Hit GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA 7968 0.1003917521019208 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 5.039746591461888E-5 0.0 0.0 1.259936647865472E-5 0.0 2 1.6379176422251135E-4 0.0 0.0 1.259936647865472E-5 0.0 3 1.763911307011661E-4 0.0 0.0 1.259936647865472E-5 0.0 4 2.1418923013713024E-4 0.0 0.0 1.259936647865472E-5 0.0 5 0.0014111290456093287 0.0 0.0 3.779809943596416E-5 0.0 6 0.0022804853326365043 0.0 0.0 3.779809943596416E-5 0.0 7 0.0024946745627736347 0.0 0.0 3.779809943596416E-5 0.0 8 0.0027214631593894196 0.0 0.0 3.779809943596416E-5 0.0 9 0.0028978542900905857 0.0 0.0 3.779809943596416E-5 0.0 10-11 0.004655465913862919 0.0 0.0 8.819556535058304E-5 0.0 12-13 0.010646464674463238 1.259936647865472E-5 0.0 1.4489271450452928E-4 1.259936647865472E-5 14-15 0.01803599311419423 1.259936647865472E-5 0.0 1.6379176422251138E-4 2.519873295730944E-5 16-17 0.021015743286396073 1.259936647865472E-5 0.0 2.6458669605174915E-4 3.779809943596416E-5 18-19 0.02269775871129648 1.259936647865472E-5 0.0 3.653816278809869E-4 5.039746591461888E-5 20-21 0.02933132516230819 1.259936647865472E-5 0.0 8.063594546339021E-4 6.929651563260096E-5 22-23 0.03180710067536384 1.259936647865472E-5 0.0 0.002904153973329913 7.559619887192832E-5 24-25 0.047449214158613674 1.259936647865472E-5 0.0 0.0079439005647918 7.559619887192832E-5 26-27 0.0962213617974861 1.259936647865472E-5 0.0 0.016744558050132124 1.0079493182923777E-4 28-29 0.10639535022899979 1.259936647865472E-5 0.0 0.05563250268649992 1.0709461506856513E-4 30-31 0.10806476628742154 1.259936647865472E-5 0.0 0.16975126456691503 1.1339429830789249E-4 32-33 0.10915461148782517 1.259936647865472E-5 0.0 0.3440697994823424 1.5119239774385665E-4 34-35 0.10997986999217704 2.519873295730944E-5 0.0 0.5660517377865206 1.5119239774385665E-4 36-37 0.11147919460313696 3.779809943596416E-5 0.0 0.8988577036369457 1.5119239774385665E-4 38-39 0.11305411541296881 3.779809943596416E-5 0.0 1.360964667974565 1.57492080983184E-4 40-41 0.11452194160773208 5.039746591461888E-5 0.0 1.9562343366250858 1.7009144746183872E-4 42-43 0.11775367910950701 5.039746591461888E-5 0.0 2.409263457414834 1.8269081394049346E-4 44-45 0.12178547638267653 5.669714915394624E-5 0.0 2.8733485222896022 1.889904971798208E-4 46-47 0.12395256741700514 6.29968323932736E-5 0.0 3.3957686539605416 1.889904971798208E-4 48-49 0.12514950723247734 6.29968323932736E-5 0.0 3.8846429723820624 1.889904971798208E-4 50-51 0.1270079137880789 6.29968323932736E-5 0.0 4.41410614991457 1.889904971798208E-4 52-53 0.12777647514327684 6.29968323932736E-5 0.0 5.0006948550612975 2.0158986365847553E-4 54-55 0.12979867346310092 7.559619887192832E-5 0.0 5.520324227057215 2.204889133764576E-4 56-57 0.1343470447618953 7.559619887192832E-5 0.0 6.061278026818256 2.2678859661578497E-4 58-59 0.13605425891975298 7.559619887192832E-5 0.0 6.611473461891389 2.3938796309443968E-4 60-61 0.13694881393973748 7.559619887192832E-5 0.0 7.125842598382468 2.3938796309443968E-4 62-63 0.1375787822636702 7.559619887192832E-5 0.0 7.656918494824243 2.3938796309443968E-4 64-65 0.13811425533901306 8.819556535058304E-5 0.0 8.206912340033718 2.3938796309443968E-4 66-67 0.13871272524674916 8.819556535058304E-5 0.0 8.762002628983808 2.3938796309443968E-4 68-69 0.1394938859684257 1.0709461506856513E-4 0.0 9.389520376136446 2.3938796309443968E-4 70-71 0.14037584162193156 1.1339429830789249E-4 0.0 10.090820013388086 2.519873295730944E-4 72-73 0.14259962980541413 1.1339429830789249E-4 0.0 10.699363114623871 3.086844787270407E-4 74-75 0.1448234179888967 1.1339429830789249E-4 0.0 11.284609987240621 3.1498416196636803E-4 76-77 0.14553528219494066 1.1339429830789249E-4 0.0 11.907119486217994 3.1498416196636803E-4 78-79 0.14595106128873628 1.1339429830789249E-4 0.0 12.529887272208178 3.1498416196636803E-4 80-81 0.14678261947632748 1.1339429830789249E-4 0.0 13.212798134084222 3.2128384520569537E-4 82-83 0.1472676950857557 1.1339429830789249E-4 0.0 13.957049011661596 3.275835284450227E-4 84-85 0.1489938082933314 1.1339429830789249E-4 0.0 14.6577501790055 3.275835284450227E-4 86-87 0.1520554543476445 1.1339429830789249E-4 0.0 15.343728986619094 3.275835284450227E-4 88 0.15270432172129522 1.5119239774385665E-4 0.0 15.87395442582356 3.527822614023322E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCTACA 8420 0.0 26.037323 2 TCTACAC 9360 0.0 25.88027 3 TACACTC 9690 0.0 23.641825 5 CTACACT 11435 0.0 21.76224 4 AGTACTT 30355 0.0 20.110811 12-13 GAGTACT 29255 0.0 19.356977 12-13 CACTCTT 11845 0.0 19.324957 7 ACACTCT 12685 0.0 18.564016 6 GTATAAG 2355 0.0 17.995733 1 ACTCTTT 14585 0.0 17.499193 8 TACTTTT 35280 0.0 17.43335 14-15 CATGGGG 16505 0.0 17.41445 4 GTACTTT 33010 0.0 17.244383 14-15 GTATTAG 3140 0.0 16.196161 1 CATGGGT 7595 0.0 15.546407 4 ACTTTTT 37355 0.0 15.49834 16-17 TGATCGC 1590 0.0 15.371921 9 GTGATCG 1625 0.0 15.040928 8 GTACTAG 2360 0.0 14.964672 1 GTATAAA 4120 0.0 14.058076 1 >>END_MODULE