FastQCFastQC Report
Fri 27 May 2016
ERR765565_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765565_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12079384
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT271360.22464721710974667No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA265710.21996982627590944No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169220.14008992511538668No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG44250.019.2285291
GTACTAG38300.018.7790411
CATGGGG266100.017.291344
GTACATA27750.017.1095731
GTACATG888400.016.5409771
TACATGG883700.015.91986752
CTAGTAC52300.015.9064473
TAGTACT46800.015.6664124
ATGGGTA62750.015.27747255
GTATCAA648200.015.2732031
GTATAAT33550.015.2726621
TATACCG24800.015.1590995
ACATGGG810550.015.1516943
GTATAAA137850.014.9705751
CATGGGT95750.014.9219524
GTACAAG61450.014.6879131
GTATATA30600.014.1334161
GTATTAT34550.014.0142521
TAGGCAT57450.013.9875345
TACATGA47700.013.8943522
Produced by FastQC (version 0.11.5)