##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765565_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12079384
Sequences flagged as poor quality	0
Sequence length	100
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.59437981274542	33.0	33.0	33.0	27.0	33.0
2	31.49093430592156	33.0	33.0	33.0	27.0	33.0
3	31.440292567899156	33.0	33.0	33.0	27.0	33.0
4	35.04821719385691	37.0	37.0	37.0	33.0	37.0
5	35.244820927954606	37.0	37.0	37.0	33.0	37.0
6	35.276474859976304	37.0	37.0	37.0	33.0	37.0
7	35.13445859490848	37.0	37.0	37.0	33.0	37.0
8	35.12038949999437	37.0	37.0	37.0	33.0	37.0
9	34.827843704612754	37.0	37.0	37.0	33.0	37.0
10-11	34.827279602999624	37.0	37.0	37.0	30.0	37.0
12-13	34.80460593023618	37.0	37.0	37.0	33.0	37.0
14-15	36.05204565895082	37.0	37.0	40.0	30.0	40.0
16-17	36.29621564311557	37.0	37.0	40.0	33.0	40.0
18-19	36.10972538003594	37.0	37.0	40.0	27.0	40.0
20-21	36.1021298354287	37.0	37.0	40.0	27.0	40.0
22-23	36.277700791696006	37.0	37.0	40.0	30.0	40.0
24-25	36.293105260996754	37.0	37.0	40.0	33.0	40.0
26-27	35.9654517564803	37.0	37.0	40.0	27.0	40.0
28-29	35.842003863773186	37.0	37.0	40.0	27.0	40.0
30-31	35.64839788187874	37.0	37.0	40.0	27.0	40.0
32-33	35.61484865453404	37.0	35.0	40.0	27.0	40.0
34-35	35.565221786144065	37.0	35.0	40.0	27.0	40.0
36-37	35.33371834192869	37.0	33.0	40.0	27.0	40.0
38-39	35.203079147082335	37.0	33.0	40.0	27.0	40.0
40-41	35.351180159518066	37.0	33.0	40.0	27.0	40.0
42-43	35.27346754602718	37.0	33.0	40.0	27.0	40.0
44-45	35.232190234204	37.0	33.0	40.0	27.0	40.0
46-47	35.198348193914526	37.0	33.0	40.0	27.0	40.0
48-49	35.04180217302472	37.0	33.0	40.0	27.0	40.0
50-51	34.88929071217539	37.0	33.0	40.0	27.0	40.0
52-53	34.772533185467076	37.0	33.0	40.0	27.0	40.0
54-55	34.64430698618406	37.0	33.0	40.0	27.0	40.0
56-57	34.442540861355184	37.0	33.0	40.0	27.0	40.0
58-59	34.25192352523936	37.0	33.0	38.5	27.0	40.0
60-61	33.978931914077734	37.0	33.0	37.0	27.0	40.0
62-63	33.755924971008454	37.0	33.0	37.0	27.0	40.0
64-65	33.55642481437795	37.0	33.0	37.0	27.0	40.0
66-67	33.332258126738914	37.0	33.0	37.0	24.5	40.0
68-69	33.13101098532839	37.0	33.0	37.0	22.0	40.0
70-71	32.892928190709064	37.0	33.0	37.0	22.0	37.0
72-73	32.650740178472674	37.0	33.0	37.0	22.0	37.0
74-75	32.42727948709967	33.0	33.0	37.0	22.0	37.0
76-77	32.22546770596911	33.0	33.0	37.0	22.0	37.0
78-79	32.03370469884888	33.0	33.0	37.0	22.0	37.0
80-81	31.87459488828238	33.0	33.0	37.0	22.0	37.0
82-83	31.731925651175587	33.0	33.0	37.0	22.0	37.0
84-85	31.560858484174357	33.0	33.0	37.0	22.0	37.0
86-87	31.352448477505142	33.0	30.0	37.0	22.0	37.0
88-89	31.201532752001263	33.0	27.0	37.0	22.0	37.0
90-91	31.09984143231145	33.0	27.0	37.0	22.0	37.0
92-93	31.007633998554894	33.0	27.0	37.0	18.5	37.0
94-95	30.870460447320824	33.0	27.0	37.0	15.0	37.0
96-97	30.76328097525503	33.0	27.0	37.0	15.0	37.0
98-99	30.620438798865905	33.0	27.0	37.0	15.0	37.0
100	30.53355767148391	33.0	27.0	37.0	15.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	9.0
7	150.0
8	1122.0
9	2231.0
10	4079.0
11	7478.0
12	13442.0
13	21672.0
14	29984.0
15	34967.0
16	41148.0
17	48674.0
18	58887.0
19	70305.0
20	82511.0
21	98154.0
22	113847.0
23	122857.0
24	130502.0
25	143351.0
26	166221.0
27	199171.0
28	243272.0
29	302435.0
30	374586.0
31	464131.0
32	591058.0
33	773107.0
34	1011762.0
35	1358767.0
36	1982798.0
37	2636390.0
38	949504.0
39	812.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.2127095970836	18.530475200194278	12.906846900183725	25.3499683025384
2	16.029950916765518	20.76053964645994	37.26186350679113	25.94764592998342
3	18.04252014743736	25.508387126904136	29.316241609271128	27.13285111638738
4	12.831561519152112	17.231432178165967	36.457928366457296	33.47907793622463
5	14.603468420210264	36.81805660399386	33.12713977667705	15.451335199118823
6	32.141785093206686	36.16633459842487	17.237219278401813	14.454661029966632
7	27.94916920308233	31.588686862797005	21.95056134658244	18.51158258753823
8	27.23987128648196	32.51480075475591	20.117435727480824	20.127892231281304
9	27.007959347224247	14.447109334640299	19.72522286834541	38.81970844979005
10-11	25.356676364528962	24.96846521756858	27.054912308406507	22.61994610949595
12-13	26.418672370789626	22.768609320339998	26.596904136824772	24.2158141720456
14-15	24.270998900000553	23.84715513088437	24.4182491456108	27.463596823504272
16-17	24.031810410536057	26.25156149596125	25.235832098052402	24.480795995450286
18-19	24.34981801130115	25.418544203003417	27.056562537979858	23.17507524771558
20-21	25.37231165285705	24.898621339635184	26.16968013567741	23.559386871830355
22-23	24.711155996606983	24.69252163413878	25.943392027780064	24.652930341474175
24-25	24.304441838343294	25.302498993384802	25.96718589724348	24.425873271028426
26-27	24.560977770858656	25.088058668238926	26.086175600610794	24.26478796029162
28-29	24.1727448355097	24.94853854776254	26.5776100981888	24.30110651853896
30-31	24.65356074564413	24.927745527105046	26.334803917630605	24.08388980962022
32-33	24.17095421753261	25.078951959402012	25.94578059982909	24.804313223236285
34-35	24.54791305575437	25.07738445691486	26.35067842599818	24.024024061332593
36-37	24.656514338398868	25.038471056679416	25.974825103702067	24.330189501219646
38-39	24.76392369668971	24.631071824042795	26.098166012160597	24.506838467106903
40-41	24.534910895410132	25.063447643109683	26.1437338171289	24.25790764435128
42-43	25.066236047626255	24.931881581696647	25.78797713903264	24.213905231644453
44-45	24.648495707871078	25.162577064056833	25.557557211301337	24.631370016770752
46-47	24.370934690130632	25.124341883893237	25.896093243531826	24.608630182444305
48-49	25.065630984832993	25.30168797814982	25.325052617034228	24.307628419982965
50-51	24.277903239564104	25.639176043175414	25.18857776022141	24.89434295703907
52-53	24.33679120155336	25.409472248921805	24.988411016329135	25.2653255331957
54-55	24.605232352908065	25.280357839439493	25.115966178407774	24.998443629244672
56-57	24.722667977108767	24.904452909188084	24.91800492475444	25.454874188948708
58-59	24.3917198095532	24.92558809290275	25.540300730567054	25.14239136697699
60-61	24.448990941922204	25.169309130333133	25.261333690525937	25.120366237218718
62-63	24.031325772587014	25.19938830988734	25.18551335551284	25.583772562012808
64-65	24.048700072503806	24.90826091369365	25.377900628228332	25.66513838557421
66-67	24.57141516010758	24.94830225508051	25.17658860201632	25.303693982795593
68-69	24.574463738813108	24.602126860684372	25.205384088766706	25.618025311735813
70-71	23.954305419183303	24.68737311792319	25.44370774797091	25.914613714922595
72-73	24.57402944329151	24.762524591542796	25.338209255526532	25.325236709639153
74-75	24.70979368519768	24.57705508986988	25.318616298244766	25.394534926687673
76-77	24.295007990686933	24.84587260706256	25.51082839595709	25.348291006293415
78-79	24.395918053268197	24.84297729267392	25.38549256259643	25.37561209146146
80-81	23.98406424964988	24.750928876239602	25.68739656444316	25.57761030966736
82-83	24.15200705368467	24.914410824977036	25.253911413605053	25.679670707733244
84-85	24.184425378062286	24.94658302522601	24.984896632115568	25.884094964596134
86-87	24.007858943033096	24.67501389604319	25.25554728222868	26.061579878695035
88-89	24.095681099538464	24.380449726219354	25.430767653347154	26.09310152089503
90-91	24.338479830919205	24.502881415525955	25.257234501748037	25.9014042518068
92-93	23.98034001788069	24.657352338402177	25.244986051357266	26.117321592359865
94-95	23.451916527531395	24.718847411504484	25.657147930853135	26.172088130110982
96-97	24.134866333668484	24.579794348027118	25.19957331417083	26.08576600413357
98-99	23.802192450936353	24.57395134094142	25.26948227578964	26.354373932332592
100	23.77265514930737	24.652228214787232	25.193463190209954	26.381653445695445
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1126.0
1	1164.0
2	1639.0
3	1989.0
4	1648.0
5	1453.5
6	1851.0
7	3370.0
8	5425.5
9	5449.0
10	3606.5
11	2426.5
12	2249.0
13	2379.5
14	2724.5
15	3234.5
16	3789.0
17	4442.5
18	5211.5
19	6237.5
20	7571.5
21	9396.0
22	11957.5
23	15098.5
24	18767.0
25	23664.0
26	30613.5
27	38072.0
28	45399.5
29	56384.0
30	70337.5
31	83571.0
32	98617.5
33	115467.5
34	131839.0
35	146256.0
36	160543.0
37	179521.0
38	195456.5
39	207821.0
40	219480.0
41	230839.5
42	248089.5
43	282284.0
44	324257.0
45	362895.0
46	411463.5
47	459774.0
48	511015.5
49	554760.0
50	595180.0
51	594263.5
52	554035.5
53	544374.0
54	533951.0
55	510357.0
56	485355.0
57	449936.5
58	423431.0
59	390768.5
60	334076.0
61	274738.5
62	226153.5
63	180834.5
64	139051.5
65	111246.0
66	85568.0
67	61204.5
68	46671.5
69	38316.5
70	33170.5
71	29699.5
72	26082.0
73	26020.0
74	21518.0
75	15902.0
76	11676.0
77	7818.5
78	5511.5
79	3430.0
80	2210.0
81	1566.0
82	1092.0
83	723.0
84	505.5
85	325.5
86	203.5
87	125.5
88	69.0
89	44.5
90	33.5
91	26.5
92	15.5
93	10.5
94	9.0
95	6.0
96	3.5
97	2.5
98	1.5
99	2.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.048528964722042114
2	0.024496282260751047
3	0.02203754761004369
4	0.023006140048201135
5	0.015704443206706567
6	0.025257910502720998
7	0.04897600738580709
8	0.006432447217507118
9	0.004172398195139752
10-11	0.002164845492121121
12-13	0.0013907993983799175
14-15	0.002375948972232359
16-17	0.006809122054568345
18-19	0.005066483522669699
20-21	0.008514507031153244
22-23	0.01849018128739015
24-25	0.025013692751219768
26-27	0.027683530882038357
28-29	0.043859852456052395
30-31	0.04176537479063502
32-33	0.03969159354483639
34-35	0.047506561592875925
36-37	0.04447246647676736
38-39	0.05413769443872303
40-41	0.05166240265232069
42-43	0.056637821928667884
44-45	0.05694412893902537
46-47	0.06147664483553135
48-49	0.0639353794862387
50-51	0.033064599982913036
52-53	2.0282491226373796E-4
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	5.381069100874681E-4
64-65	1.4901422125499116E-4
66-67	3.063070103574818E-4
68-69	0.0010389602648611883
70-71	7.036782670374582E-5
72-73	2.3179989972998624E-4
74-75	2.897498746624828E-5
76-77	7.864639455124533E-5
78-79	4.139283923749754E-6
80-81	2.4835703542498526E-5
82-83	1.9454634441623845E-4
84-85	0.0010306816970136886
86-87	0.0033610985460848
88-89	0.0023138597133761127
90-91	0.003518391335187291
92-93	0.003568062742272288
94-95	0.0025787738844960965
96-97	0.0019785777155523826
98-99	0.003990269702494763
100	0.002831270203844832
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.2079384E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.094949894768654
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.99671570844387	30.8198627431071
2	12.787450707932285	10.509992922486026
3	4.663841995993753	5.749810594274433
4	2.1878110243503013	3.5963193769959245
5	1.215493549249425	2.497532325190982
6	0.8353615590789796	2.059748484861989
7	0.5385523088418387	1.5492246103297138
8	0.40315560005756745	1.3254127347328901
9	0.305781059066196	1.1309451570985158
>10	1.7837209791505795	13.65261912817142
>50	0.15335033793967298	4.3434268709019275
>100	0.10368114475860382	8.647676757889519
>500	0.014283569050074942	4.091992170043556
>1k	0.01019462878680444	7.881981196190112
>5k	5.22264913762436E-4	1.4478998331256623
>10k+	8.356238620198976E-5	0.6955550946004635
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	27136	0.22464721710974667	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	26571	0.21996982627590944	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16922	0.14008992511538668	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.278567847499509E-6
2	0.0	0.0	0.0	0.0	8.278567847499509E-6
3	0.0	0.0	0.0	0.0	8.278567847499509E-6
4	0.0	8.278567847499509E-6	0.0	0.0	8.278567847499509E-6
5	0.0	8.278567847499509E-6	0.0	0.0	8.278567847499509E-6
6	0.0	8.278567847499509E-6	0.0	0.0	1.6557135694999017E-5
7	0.0	8.278567847499509E-6	0.0	0.0	1.6557135694999017E-5
8	0.0	8.278567847499509E-6	0.0	0.0	2.4835703542498526E-5
9	0.0	8.278567847499509E-6	0.0	1.6557135694999017E-5	5.794997493249656E-5
10-11	0.0	8.278567847499509E-6	0.0	2.069641961874877E-5	6.208925885624631E-5
12-13	4.139283923749754E-6	8.278567847499509E-6	0.0	6.208925885624631E-5	6.622854277999607E-5
14-15	8.278567847499509E-6	1.6557135694999017E-5	0.0	8.692496239874484E-5	1.0348209809374386E-4
16-17	8.278567847499509E-6	1.6557135694999017E-5	0.0	1.1176066594124337E-4	1.1589994986499312E-4
18-19	8.278567847499509E-6	1.6557135694999017E-5	0.0	1.2417851771249263E-4	1.3245708555999214E-4
20-21	1.6557135694999017E-5	1.6557135694999017E-5	0.0	2.35939183653736E-4	1.4901422125499116E-4
22-23	1.6557135694999017E-5	1.6557135694999017E-5	8.278567847499509E-6	0.0010886316719461854	1.7384992479748968E-4
24-25	4.139283923749754E-5	1.6557135694999017E-5	8.278567847499509E-6	0.0029761451411760734	2.0696419618748772E-4
26-27	5.3810691008746806E-5	1.6557135694999017E-5	8.278567847499509E-6	0.004983697844194704	2.1524276403498722E-4
28-29	6.622854277999607E-5	1.6557135694999017E-5	8.278567847499509E-6	0.016234271548946536	2.1524276403498722E-4
30-31	7.450711062749558E-5	1.6557135694999017E-5	8.278567847499509E-6	0.04672423693128722	2.1524276403498722E-4
32-33	9.93428141699941E-5	1.6557135694999017E-5	8.278567847499509E-6	0.09817139681957292	2.4007846757748575E-4
34-35	1.1176066594124337E-4	1.6557135694999017E-5	8.278567847499509E-6	0.1653768106055739	2.649141711199843E-4
36-37	1.2417851771249263E-4	1.6557135694999017E-5	8.278567847499509E-6	0.2677785555952191	2.8561059073873305E-4
38-39	1.2417851771249263E-4	1.6557135694999017E-5	8.278567847499509E-6	0.4206091966279075	3.104462942812316E-4
40-41	1.2417851771249263E-4	1.6557135694999017E-5	8.278567847499509E-6	0.602021593154088	3.1458557820498133E-4
42-43	1.2417851771249263E-4	1.6557135694999017E-5	8.278567847499509E-6	0.7672907823776445	3.352819978237301E-4
44-45	1.2417851771249263E-4	1.6557135694999017E-5	8.278567847499509E-6	0.9617088089922465	3.5597841744247887E-4
46-47	1.3245708555999214E-4	1.6557135694999017E-5	8.278567847499509E-6	1.1786735151395136	3.5597841744247887E-4
48-49	1.3245708555999214E-4	1.6557135694999017E-5	8.278567847499509E-6	1.4016815758154555	3.725355531374779E-4
50-51	1.3245708555999214E-4	1.6557135694999017E-5	8.278567847499509E-6	1.6407583366833938	3.7667483706122764E-4
52-53	1.3245708555999214E-4	1.6557135694999017E-5	8.278567847499509E-6	1.8828857498031357	4.1392839237497543E-4
54-55	1.3245708555999214E-4	1.6557135694999017E-5	8.278567847499509E-6	2.12781959742318	4.4704266376497347E-4
56-57	1.3245708555999214E-4	1.6557135694999017E-5	8.278567847499509E-6	2.4051019489073284	4.4704266376497347E-4
58-59	1.3245708555999214E-4	1.6557135694999017E-5	8.278567847499509E-6	2.700489528273958	4.55321231612473E-4
60-61	1.3245708555999214E-4	1.6557135694999017E-5	8.278567847499509E-6	3.0004717127959504	4.635997994599725E-4
62-63	1.3245708555999214E-4	1.6557135694999017E-5	8.278567847499509E-6	3.308513083117483	4.88435503002471E-4
64-65	1.3245708555999214E-4	1.6557135694999017E-5	8.278567847499509E-6	3.6311951006773193	4.925747869262208E-4
66-67	1.3245708555999214E-4	1.6557135694999017E-5	8.278567847499509E-6	3.9894418457100134	5.21549774392469E-4
68-69	1.3245708555999214E-4	2.069641961874877E-5	8.278567847499509E-6	4.378956741502712	5.463854779349676E-4
70-71	1.3245708555999214E-4	2.4835703542498526E-5	8.278567847499509E-6	4.81030737991275	5.712211814774661E-4
72-73	1.365963694837419E-4	2.4835703542498526E-5	8.278567847499509E-6	5.223482422613603	6.250318724862129E-4
74-75	1.448749373312414E-4	2.4835703542498526E-5	8.278567847499509E-6	5.650234316584356	6.291711564099627E-4
76-77	1.4901422125499116E-4	2.4835703542498526E-5	8.278567847499509E-6	6.107637608010474	6.374497242574622E-4
78-79	1.4901422125499116E-4	2.4835703542498526E-5	8.278567847499509E-6	6.581676681526144	6.374497242574622E-4
80-81	1.4901422125499116E-4	3.3114271389998035E-5	8.278567847499509E-6	7.096810565836801	6.622854277999607E-4
82-83	1.4901422125499116E-4	3.3114271389998035E-5	8.278567847499509E-6	7.643183625919997	6.995389831137085E-4
84-85	1.4901422125499116E-4	4.139283923749754E-5	8.278567847499509E-6	8.180988368281032	7.119568348849577E-4
86-87	1.4901422125499116E-4	4.139283923749754E-5	8.278567847499509E-6	8.747275523321388	7.119568348849577E-4
88	1.4901422125499116E-4	4.967140708499705E-5	8.278567847499509E-6	9.186950261702087	7.119568348849577E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATAAG	4425	0.0	19.228529	1
GTACTAG	3830	0.0	18.779041	1
CATGGGG	26610	0.0	17.29134	4
GTACATA	2775	0.0	17.109573	1
GTACATG	88840	0.0	16.540977	1
TACATGG	88370	0.0	15.9198675	2
CTAGTAC	5230	0.0	15.906447	3
TAGTACT	4680	0.0	15.666412	4
ATGGGTA	6275	0.0	15.2774725	5
GTATCAA	64820	0.0	15.273203	1
GTATAAT	3355	0.0	15.272662	1
TATACCG	2480	0.0	15.159099	5
ACATGGG	81055	0.0	15.151694	3
GTATAAA	13785	0.0	14.970575	1
CATGGGT	9575	0.0	14.921952	4
GTACAAG	6145	0.0	14.687913	1
GTATATA	3060	0.0	14.133416	1
GTATTAT	3455	0.0	14.014252	1
TAGGCAT	5745	0.0	13.987534	5
TACATGA	4770	0.0	13.894352	2
>>END_MODULE