FastQCFastQC Report
Fri 27 May 2016
ERR765564_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765564_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10285982
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA831750.8086247866270814No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT814450.7918057799440054No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG525080.5104811577543107No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG389530.3786998655062784No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG305920.29741448118419805No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC275270.26761664564452864No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT271990.26442783975317086No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC262370.25507530540107887No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA257150.2500004374886131No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC245540.23871323126950836No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT240160.23348281185014713No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC235280.22873849088983433No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166150.1615305179417969No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG155850.15151688968539903No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT119680.1163525271578348No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC115060.1118609773962272No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC114420.1112387713686452No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA112530.1094013191934421No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA103850.10096264994436117No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA103730.10084598631418953No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTAC106700.023.9161621
GTACAAA36900.023.3067021
TCTACAC128300.022.60013
TACACTC126950.021.109085
CTACACT156850.020.9462244
ATCTACA127250.020.1262632
CGTTAAC13600.020.0421891
CTATTGA50600.019.6992879
GTACAAG39700.019.413751
GTACTAG38750.019.4045831
AGGGCTA54750.019.063215
TAGTACT42000.019.0244774
GTATAAG35850.018.4835781
GTCCTAC32550.018.4805561
GTACATA18500.018.2901331
ATTTAGG59000.018.0016571
GTGATCG29900.017.9266388
CATGGGG235800.017.6206344
GAACAAA54900.017.4628011
AGTACTC28000.017.2944345
Produced by FastQC (version 0.11.5)