##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765564_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10285982 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.02468359365202 33.0 33.0 33.0 33.0 33.0 2 32.00541426185657 33.0 33.0 33.0 33.0 33.0 3 31.984027582393203 33.0 33.0 33.0 33.0 33.0 4 35.73442457900471 37.0 37.0 37.0 33.0 37.0 5 35.76048947003796 37.0 37.0 37.0 33.0 37.0 6 35.68895998456929 37.0 37.0 37.0 33.0 37.0 7 35.64396855837391 37.0 37.0 37.0 33.0 37.0 8 35.62294207786869 37.0 37.0 37.0 33.0 37.0 9 35.60217974326613 37.0 37.0 37.0 33.0 37.0 10-11 35.58520314346263 37.0 37.0 37.0 33.0 37.0 12-13 35.55896889572624 37.0 37.0 37.0 33.0 37.0 14-15 37.165610585357825 40.0 37.0 40.0 33.0 40.0 16-17 37.20519372870767 40.0 37.0 40.0 33.0 40.0 18-19 37.2487482478581 40.0 37.0 40.0 33.0 40.0 20-21 37.268893626296446 40.0 37.0 40.0 33.0 40.0 22-23 37.26019965813667 40.0 37.0 40.0 33.0 40.0 24-25 37.241193159778035 40.0 37.0 40.0 33.0 40.0 26-27 37.21397913198759 40.0 37.0 40.0 33.0 40.0 28-29 37.17688257669516 40.0 37.0 40.0 33.0 40.0 30-31 37.076525945699686 38.5 37.0 40.0 33.0 40.0 32-33 36.94178951508957 37.0 37.0 40.0 33.0 40.0 34-35 36.83810646372899 37.0 37.0 40.0 33.0 40.0 36-37 36.76953036666796 37.0 37.0 40.0 33.0 40.0 38-39 36.65490334321021 37.0 37.0 40.0 33.0 40.0 40-41 36.48415250969718 37.0 37.0 40.0 33.0 40.0 42-43 36.356368988396056 37.0 37.0 40.0 33.0 40.0 44-45 36.168481093978194 37.0 37.0 40.0 33.0 40.0 46-47 35.927046343266014 37.0 37.0 40.0 27.0 40.0 48-49 35.69032528931122 37.0 37.0 40.0 27.0 40.0 50-51 35.46639241639738 37.0 37.0 40.0 27.0 40.0 52-53 35.291991420945514 37.0 33.0 40.0 27.0 40.0 54-55 35.15114283692116 37.0 33.0 40.0 27.0 40.0 56-57 34.97777407154709 37.0 33.0 40.0 27.0 40.0 58-59 34.804918771975295 37.0 33.0 40.0 27.0 40.0 60-61 34.51279780579044 37.0 33.0 37.0 27.0 40.0 62-63 34.27394696004718 37.0 33.0 37.0 27.0 40.0 64-65 34.067451459666174 37.0 33.0 37.0 27.0 40.0 66-67 33.843789003325114 37.0 33.0 37.0 27.0 40.0 68-69 33.605239976115065 37.0 33.0 37.0 27.0 40.0 70-71 33.36845028505786 37.0 33.0 37.0 27.0 38.5 72-73 33.105159672649634 37.0 33.0 37.0 27.0 37.0 74-75 32.90994136485948 37.0 33.0 37.0 27.0 37.0 76-77 32.69780794872089 37.0 33.0 37.0 22.0 37.0 78-79 32.51661499115981 33.0 33.0 37.0 22.0 37.0 80-81 32.34790168794773 33.0 33.0 37.0 22.0 37.0 82-83 32.182977911102704 33.0 33.0 37.0 22.0 37.0 84-85 32.02163527021533 33.0 33.0 37.0 22.0 37.0 86-87 31.880060114824232 33.0 33.0 37.0 22.0 37.0 88-89 31.75906092388651 33.0 33.0 37.0 22.0 37.0 90-91 31.635483320892455 33.0 33.0 37.0 22.0 37.0 92-93 31.460446946144764 33.0 33.0 37.0 22.0 37.0 94-95 31.380825185188932 33.0 33.0 37.0 22.0 37.0 96-97 31.220382604208332 33.0 33.0 37.0 22.0 37.0 98-99 31.092599325956435 33.0 33.0 37.0 15.0 37.0 100 30.98897305089587 33.0 27.0 37.0 15.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 9.0 7 328.0 8 2181.0 9 3188.0 10 3171.0 11 5141.0 12 11263.0 13 21745.0 14 29138.0 15 29770.0 16 31287.0 17 34712.0 18 40944.0 19 47869.0 20 58073.0 21 73876.0 22 83304.0 23 78189.0 24 74242.0 25 76051.0 26 83280.0 27 97369.0 28 121183.0 29 156841.0 30 205838.0 31 275544.0 32 377806.0 33 527949.0 34 770291.0 35 1232674.0 36 2157175.0 37 2791211.0 38 784041.0 39 299.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.46383069840476 18.912333850040653 12.95657295564722 24.66726249590736 2 15.474610342689981 21.50089135640956 37.13064321554103 25.89385508535943 3 18.410911616042288 26.901712655151215 29.22213841056937 25.465237318237122 4 12.980133416171533 17.821306723992254 35.974753794563206 33.223806065273 5 13.687451354632204 37.47560864743879 33.17129635256364 15.665643645365362 6 31.04945583328691 36.850945115284745 17.304665787552967 14.794933263875379 7 26.22985072595237 32.6986877145699 21.97359094846822 19.09787061100951 8 27.181536052073874 32.41803346031013 20.33291716419151 20.067513323424482 9 27.405054239233657 14.776096721650491 19.616466932749535 38.202382106366315 10-11 24.99099741450996 26.117076691525764 26.19909430537723 22.692831588587044 12-13 26.67070927971761 24.03146255749686 26.090413911256817 23.207414251528718 14-15 24.897996885675713 23.8924289250039 24.566952867526485 26.642621321793904 16-17 23.411225207166186 26.63566080794837 26.007514595915467 23.945599388969978 18-19 24.272756274163797 25.94598005572713 27.806850468124818 21.97441320198426 20-21 25.066724182778543 25.181678629502663 26.594833748443914 23.15676343927488 22-23 24.161125665183885 25.50919959286296 25.53980924407578 24.789865497877376 24-25 23.989287590340748 26.100551244205395 25.386089209218948 24.52407195623491 26-27 24.441603102676506 25.250482970307985 25.61395877765419 24.693955149361322 28-29 23.87414068364009 24.79951735709552 27.29615704191349 24.0301849173509 30-31 25.059568742000067 24.83128571480667 26.51663196229805 23.592513580895215 32-33 23.22694026001455 25.776638091810185 26.399902099631323 24.596519548543945 34-35 23.40029808745769 25.813733111533217 26.965607451029445 23.820361349979652 36-37 24.332906083914406 25.664979221055244 26.032775731195912 23.969338963834435 38-39 24.83609506811754 24.522810672193735 26.12354347912956 24.51755078055917 40-41 23.91813779552729 25.263709313380282 26.398195727493206 24.41995716359922 42-43 25.26091556871656 24.93977263568402 26.03331529194145 23.765996503657977 44-45 24.701090791291694 25.00030989908082 26.254781378229858 24.04381793139763 46-47 23.8004312148442 25.14415516422534 26.978517383819373 24.07689623711109 48-49 24.821493407274488 25.38880462814173 26.393723428936816 23.395978535646965 50-51 23.72878241366213 25.859171176104358 25.569915417690204 24.842130992543307 52-53 23.280184578080156 26.208271901629924 24.79242550743511 25.719118012854807 54-55 23.961790635703757 25.726443147094685 24.70608817150638 25.605678045695175 56-57 24.37343712663932 24.674583239847568 24.982827383911342 25.96915224960177 58-59 23.900243934607808 24.551925664821102 26.37929860183277 25.168531798738318 60-61 24.203878444493913 25.221930868437862 25.503905955088456 25.07028473197977 62-63 22.997315067795192 25.457669947904748 25.627120255850812 25.917894728449248 64-65 22.643297059387454 25.403764953231228 25.73664073003698 26.216297257344333 66-67 24.178238476387246 25.220377145964267 25.06549860815458 25.535885769493905 68-69 24.244314533392362 24.50442561205731 25.233142060841796 26.01811779370854 70-71 23.368777723920708 25.005676705876244 25.452292847146424 26.173252723056624 72-73 24.587557452016732 24.821055399052998 25.3873328895106 25.20405425941967 74-75 24.54560082133687 24.689884081445353 25.548170415733733 25.216344681484042 76-77 23.687212358723126 25.140389652993804 26.03305841372429 25.13933957455878 78-79 23.773383362506433 25.225073917928253 25.91380953732802 25.087733182237297 80-81 23.37295440429943 25.200150350968002 25.962060492735823 25.46483475199674 82-83 23.55391497759145 25.208889804065915 25.44758767567656 25.789607542666076 84-85 23.82655541039387 25.260583701658025 24.895550186614926 26.017310701333184 86-87 23.570397976563214 24.64108677039319 25.481168287700502 26.307346965343086 88-89 23.781651728996295 24.019123549512475 26.05541086922114 26.143813852270085 90-91 23.931550517443213 24.431693286212347 25.70108604401984 25.9356701523246 92-93 23.472093493641797 24.785563313648414 25.70783975810137 26.034503434608425 94-95 22.61453111423033 24.9014516530859 26.197072388209346 26.28694484447442 96-97 23.47255178619813 24.851240489769634 25.41163886824844 26.2645688557838 98-99 23.416971938606977 24.710832038671132 25.343256233057765 26.52893978966412 100 23.237360836216986 24.900233566965117 25.397984088511798 26.464421508306103 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 408.0 1 385.5 2 392.0 3 425.0 4 354.5 5 312.5 6 390.0 7 755.5 8 1217.5 9 1222.0 10 940.5 11 837.0 12 998.5 13 1267.0 14 1563.0 15 2002.0 16 2577.5 17 3194.5 18 4037.0 19 5098.5 20 6229.5 21 7578.0 22 9603.0 23 11898.5 24 14384.0 25 17840.0 26 23291.5 27 29532.0 28 35900.5 29 47806.5 30 62409.0 31 75361.5 32 93593.0 33 113109.0 34 132580.0 35 148232.0 36 161458.5 37 184106.0 38 195298.0 39 195680.0 40 194298.5 41 192671.0 42 199932.0 43 222256.0 44 254313.5 45 286411.5 46 328389.5 47 380573.5 48 467600.0 49 555320.0 50 650069.0 51 650116.0 52 535129.5 53 472411.0 54 428329.0 55 381705.5 56 357384.0 57 323113.0 58 295734.0 59 276591.5 60 237650.0 61 195323.5 62 159985.5 63 128386.5 64 100232.5 65 80683.0 66 64079.0 67 48211.5 68 38317.5 69 32944.0 70 29298.5 71 25735.5 72 21706.0 73 20915.0 74 16584.5 75 12146.0 76 9122.0 77 6022.5 78 4181.0 79 2605.0 80 1731.5 81 1200.5 82 831.5 83 590.0 84 403.5 85 246.5 86 158.5 87 93.0 88 58.0 89 40.5 90 26.0 91 18.5 92 12.5 93 11.0 94 8.0 95 2.5 96 3.0 97 3.5 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02211747988670406 2 0.005191531542637348 3 0.00987752068786432 4 0.00847755712580481 5 0.010567780499713105 6 0.006056786799743573 7 0.00847755712580481 8 0.008759494232052904 9 0.006951207964392704 10-11 0.02746456293623691 12-13 0.02944784464915455 14-15 0.012123295568668115 16-17 0.003879065703206558 18-19 0.0119094122466868 20-21 0.0016381518069932457 22-23 1.0208067640017258E-4 24-25 0.0033151914907103663 26-27 0.0014874612846882292 28-29 0.0011326094095828673 30-31 0.02619584595812048 32-33 0.020002951589843342 34-35 0.007247728024412254 36-37 0.007884517005765711 38-39 0.005823459539400322 40-41 0.014626702632767585 42-43 0.0036457384428633063 44-45 0.003650599427453791 46-47 0.012808694395926419 48-49 0.01718844151195287 50-51 0.007714382545098757 52-53 0.021427220074855275 54-55 0.027182625829988812 56-57 0.013994774636004613 58-59 0.01024209453215065 60-61 0.008297700695956886 62-63 0.015078774199682636 64-65 0.0088858798314055 66-67 0.017441212710658058 68-69 0.005526939479380773 70-71 0.005128338742961051 72-73 0.010645556253160856 74-75 0.003033254384462271 76-77 0.010071960071483695 78-79 0.0024304922952422046 80-81 0.006941485995211735 82-83 0.0017110665758505118 84-85 0.008740050293690967 86-87 0.0030478373382337243 88-89 0.0012638559935259463 90-91 0.0011569143325352894 92-93 1.9930036820986076E-4 94-95 0.0028631199237953166 96-97 8.263673803823496E-4 98-99 0.004671406191455517 100 0.006892876149306892 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.0285982E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.225432237753292 #Duplication Level Percentage of deduplicated Percentage of total 1 75.65700517303951 23.624226883420953 2 12.85089285772768 8.025493682472069 3 4.371277559473559 4.094850936772596 4 2.0421232831810756 2.5506472880043582 5 1.0732817201933285 1.6756842812958033 6 0.6993585805610789 1.3102664380320777 7 0.47090382352179705 1.0292922802315165 8 0.36372381193109965 0.90859465941695 9 0.2656272475892088 0.746489305808798 >10 1.7621493490851774 10.785201053478154 >50 0.2002983764065203 4.370233668918568 >100 0.18520259957281185 12.257308239993627 >500 0.03281675277682792 7.2169664073107525 >1k 0.023649607285528184 13.64509409811322 >5k 0.0010323241223856648 2.1445106671378786 >10k+ 6.569335324272412E-4 5.615140109592729 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 83175 0.8086247866270814 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 81445 0.7918057799440054 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 52508 0.5104811577543107 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 38953 0.3786998655062784 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 30592 0.29741448118419805 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 27527 0.26761664564452864 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 27199 0.26442783975317086 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 26237 0.25507530540107887 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 25715 0.2500004374886131 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 24554 0.23871323126950836 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 24016 0.23348281185014713 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 23528 0.22873849088983433 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16615 0.1615305179417969 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 15585 0.15151688968539903 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 11968 0.1163525271578348 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 11506 0.1118609773962272 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 11442 0.1112387713686452 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 11253 0.1094013191934421 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 10385 0.10096264994436117 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 10373 0.10084598631418953 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 7.97201472839443E-4 0.0 0.0 4.860984590484409E-5 3.888787672387527E-5 2 0.0015652370381359795 0.0 0.0 7.777575344775054E-5 3.888787672387527E-5 3 0.001594402945678886 0.0 0.0 7.777575344775054E-5 3.888787672387527E-5 4 0.0017110665758505118 0.0 0.0 7.777575344775054E-5 3.888787672387527E-5 5 0.005716517878409664 0.0 0.0 1.4582953771453226E-4 3.888787672387527E-5 6 0.012891331133964653 0.0 0.0 3.4999089051487744E-4 5.8331815085812905E-5 7 0.014845446939339384 0.0 0.0 4.083227056006903E-4 6.805378426678172E-5 8 0.01826758009104041 0.0 0.0 4.860984590484409E-4 6.805378426678172E-5 9 0.02041613528003452 9.721969180968817E-6 0.0 5.055423974103785E-4 6.805378426678172E-5 10-11 0.04122114932730779 9.721969180968817E-6 0.0 9.138651030110688E-4 7.777575344775054E-5 12-13 0.07752298224904534 9.721969180968817E-6 0.0 0.001842313159793591 1.5069052230501666E-4 14-15 0.12057672276696577 2.9165907542906452E-5 0.0 0.002838815000842895 1.8957839902889194E-4 16-17 0.13968525319215996 2.9165907542906452E-5 0.0 0.003388106259567633 2.236052911622828E-4 18-19 0.15053497079812117 3.402689213339086E-5 0.0 0.0037283751809015415 2.5277119870518923E-4 20-21 0.18861106309538556 5.8331815085812905E-5 0.0 0.004826957698351018 3.013810446100333E-4 22-23 0.1990913458724699 5.8331815085812905E-5 0.0 0.006367889813534576 3.4999089051487744E-4 24-25 0.24875602543344916 5.8331815085812905E-5 0.0 0.012886470149374166 3.4999089051487744E-4 26-27 0.37982761393127074 5.8331815085812905E-5 0.0 0.034124111825200545 3.840177826482683E-4 28-29 0.40621789927301055 5.8331815085812905E-5 0.0 0.057422810967392324 3.9373975182923715E-4 30-31 0.4159107025464365 5.8331815085812905E-5 0.0 0.12234612115790208 3.986007364197215E-4 32-33 0.42455839413290825 5.8331815085812905E-5 0.0 0.23407099098559575 4.083227056006903E-4 34-35 0.43064434684019476 7.777575344775054E-5 0.0 0.38254004333276104 4.2776664396262796E-4 36-37 0.44243223447211943 1.0694166099065699E-4 0.0 0.6085417998981526 4.374886131435968E-4 38-39 0.4538944361364816 1.0694166099065699E-4 0.0 0.9693581030960388 4.374886131435968E-4 40-41 0.46454971435882353 1.2638559935259462E-4 0.0 1.4937902866250399 4.5207156691505E-4 42-43 0.49300105716692877 1.3610756853356344E-4 0.0 1.8780559794874228 4.5693255150553445E-4 44-45 0.5250641115257639 1.5069052230501666E-4 0.0 2.264304954062723 4.6665452068650324E-4 46-47 0.5359624389776299 1.5555150689550108E-4 0.0 2.718972286749092 5.055423974103786E-4 48-49 0.5442844445965392 1.5555150689550108E-4 0.0 3.1610642522998775 5.34708304953285E-4 50-51 0.5598930661165846 1.652734760764699E-4 0.0 3.66504141267212 5.444302741342538E-4 52-53 0.5642533692942493 1.652734760764699E-4 0.0 4.244976318255272 5.881791354486135E-4 54-55 0.5798182419529803 1.701344606669543E-4 0.0 4.741452979404397 6.222060275820043E-4 56-57 0.6142631787611528 1.7499544525743872E-4 0.0 5.260304752623522 6.41649965943942E-4 58-59 0.6242962509559127 1.7499544525743872E-4 0.0 5.815186143627317 6.562329197153952E-4 60-61 0.6315196740573724 1.7499544525743872E-4 0.0 6.350079165995041 6.708158734868485E-4 62-63 0.6376542366105638 1.7499544525743872E-4 0.0 6.905077220629007 7.097037502107237E-4 64-65 0.6421943962180763 1.7499544525743872E-4 0.0 7.484380198215396 7.291476885726613E-4 66-67 0.6485865909545632 1.7499544525743872E-4 0.0 8.065248412839921 7.485916269345989E-4 68-69 0.6544392164015065 1.7985642984792315E-4 0.0 8.727056881880603 7.583135961155678E-4 70-71 0.6620077694088906 1.9930036820986076E-4 0.0 9.533751857625262 7.631745807060522E-4 72-73 0.6830558326856881 2.1388332198131398E-4 0.0 10.237705063065441 8.166454112013806E-4 74-75 0.7043761110995528 2.2846627575276722E-4 0.0 10.91143266632199 8.506723033347716E-4 76-77 0.7106759471288207 2.3332726034325162E-4 0.0 11.632102797768848 8.555332879252559E-4 78-79 0.7151286090137043 2.3332726034325162E-4 0.0 12.346857110969083 8.701162416967092E-4 80-81 0.7240630986910146 2.3332726034325162E-4 0.0 13.158374183427505 8.798382108776779E-4 82-83 0.7272907924590963 2.3332726034325162E-4 0.0 14.075953078665702 8.992821492396156E-4 84-85 0.7389182675995349 2.3332726034325162E-4 0.0 14.923188665895001 9.235870721920377E-4 86-87 0.7601267433678185 2.3332726034325162E-4 0.0 15.769831213004261 9.624749489159129E-4 88 0.7650023109120743 2.3332726034325162E-4 0.0 16.42558775622979 9.916408564588195E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCTAC 10670 0.0 23.916162 1 GTACAAA 3690 0.0 23.306702 1 TCTACAC 12830 0.0 22.6001 3 TACACTC 12695 0.0 21.10908 5 CTACACT 15685 0.0 20.946224 4 ATCTACA 12725 0.0 20.126263 2 CGTTAAC 1360 0.0 20.042189 1 CTATTGA 5060 0.0 19.699287 9 GTACAAG 3970 0.0 19.41375 1 GTACTAG 3875 0.0 19.404583 1 AGGGCTA 5475 0.0 19.06321 5 TAGTACT 4200 0.0 19.024477 4 GTATAAG 3585 0.0 18.483578 1 GTCCTAC 3255 0.0 18.480556 1 GTACATA 1850 0.0 18.290133 1 ATTTAGG 5900 0.0 18.001657 1 GTGATCG 2990 0.0 17.926638 8 CATGGGG 23580 0.0 17.620634 4 GAACAAA 5490 0.0 17.462801 1 AGTACTC 2800 0.0 17.294434 5 >>END_MODULE