FastQCFastQC Report
Fri 27 May 2016
ERR765562_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765562_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14318992
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA227080.1585865820722576No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT206640.144311834240846No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188270.13148271889529656No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA616800.023.3269881
CATGGGG356300.020.6909264
GTACATG986750.020.6822051
TACATGG973950.020.3938752
GTATAAG55900.019.657051
TAGTACT60300.019.3900134
CAACGCA679350.019.3742795
AACGCAG699100.019.1963656
ATCAACG692800.019.0455933
ACATGGG959800.019.021363
GTACAAG64250.018.564141
TATCAAC773450.018.5227742
GTACATA36550.018.5007511
TATTCGC11750.017.9836069
TCAACGC741300.017.8311944
ACGCAGA739150.017.6416577
CGCAGAG739350.017.5416188
GTACTAG50850.017.1765611
CATGGGT127700.016.8043374
CTAGTAC74950.016.7903293
Produced by FastQC (version 0.11.5)