##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765562_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14318992
Sequences flagged as poor quality	0
Sequence length	100
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.18269204983144	33.0	33.0	33.0	33.0	33.0
2	32.144120829175684	33.0	33.0	33.0	33.0	33.0
3	32.142789939403556	33.0	33.0	33.0	33.0	33.0
4	35.94259735601501	37.0	37.0	37.0	33.0	37.0
5	35.9924630169498	37.0	37.0	37.0	33.0	37.0
6	35.954735710446656	37.0	37.0	37.0	33.0	37.0
7	35.89635848668677	37.0	37.0	37.0	33.0	37.0
8	35.862133172502645	37.0	37.0	37.0	33.0	37.0
9	35.86132417700911	37.0	37.0	37.0	33.0	37.0
10-11	35.837388972631594	37.0	37.0	37.0	33.0	37.0
12-13	35.816593514403806	37.0	37.0	37.0	33.0	37.0
14-15	37.66395207847033	40.0	37.0	40.0	33.0	40.0
16-17	37.71380907957767	40.0	37.0	40.0	33.0	40.0
18-19	37.75962190634648	40.0	37.0	40.0	33.0	40.0
20-21	37.776993310702316	40.0	37.0	40.0	33.0	40.0
22-23	37.76534591261731	40.0	37.0	40.0	33.0	40.0
24-25	37.76507256935405	40.0	37.0	40.0	33.0	40.0
26-27	37.74497471609733	40.0	37.0	40.0	33.0	40.0
28-29	37.69716782438317	40.0	37.0	40.0	33.0	40.0
30-31	37.63121028351716	40.0	37.0	40.0	33.0	40.0
32-33	37.55294374073259	40.0	37.0	40.0	33.0	40.0
34-35	37.44940185035371	40.0	37.0	40.0	33.0	40.0
36-37	37.346152718012554	40.0	37.0	40.0	33.0	40.0
38-39	37.22599680899326	40.0	37.0	40.0	33.0	40.0
40-41	37.12036632885891	40.0	37.0	40.0	33.0	40.0
42-43	36.96490741108033	40.0	37.0	40.0	33.0	40.0
44-45	36.80373237864788	40.0	37.0	40.0	33.0	40.0
46-47	36.609296031452494	37.0	37.0	40.0	33.0	40.0
48-49	36.41879725192946	37.0	37.0	40.0	33.0	40.0
50-51	36.20087049423591	37.0	37.0	40.0	33.0	40.0
52-53	36.05323346084697	37.0	37.0	40.0	33.0	40.0
54-55	35.881108251195336	37.0	37.0	40.0	30.0	40.0
56-57	35.70561964138258	37.0	37.0	40.0	27.0	40.0
58-59	35.50548924812584	37.0	35.0	40.0	27.0	40.0
60-61	35.35087515238503	37.0	33.0	40.0	27.0	40.0
62-63	35.14980453931394	37.0	33.0	38.5	27.0	40.0
64-65	34.95990946848772	37.0	33.0	37.0	27.0	40.0
66-67	34.78262020818225	37.0	33.0	37.0	27.0	40.0
68-69	34.56655224054877	37.0	33.0	37.0	27.0	40.0
70-71	34.391308620048115	37.0	33.0	37.0	27.0	40.0
72-73	34.199279320779	37.0	33.0	37.0	27.0	38.5
74-75	33.99444960930211	37.0	33.0	37.0	27.0	37.0
76-77	33.82041529180266	37.0	33.0	37.0	27.0	37.0
78-79	33.67365307557962	37.0	33.0	37.0	27.0	37.0
80-81	33.51975289182367	37.0	33.0	37.0	27.0	37.0
82-83	33.394466733412514	37.0	33.0	37.0	27.0	37.0
84-85	33.258999236817786	37.0	33.0	37.0	27.0	37.0
86-87	33.15991879875343	37.0	33.0	37.0	27.0	37.0
88-89	33.09121843213545	37.0	33.0	37.0	27.0	37.0
90-91	32.985801863706605	37.0	33.0	37.0	27.0	37.0
92-93	32.915399212458524	37.0	33.0	37.0	27.0	37.0
94-95	32.82557193970078	37.0	33.0	37.0	27.0	37.0
96-97	32.75669352284015	37.0	33.0	37.0	27.0	37.0
98-99	32.67186632271322	37.0	33.0	37.0	22.0	37.0
100	32.60469088885586	37.0	33.0	37.0	22.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	8.0
4	39.0
5	108.0
6	417.0
7	1111.0
8	2142.0
9	3340.0
10	3843.0
11	4833.0
12	7303.0
13	12658.0
14	20985.0
15	28390.0
16	33176.0
17	40649.0
18	48803.0
19	58510.0
20	68221.0
21	82718.0
22	99194.0
23	100747.0
24	93075.0
25	92823.0
26	100928.0
27	115728.0
28	139297.0
29	176803.0
30	226133.0
31	291803.0
32	391475.0
33	537998.0
34	745054.0
35	1117127.0
36	2175363.0
37	4715809.0
38	2778125.0
39	4256.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.24529914685154	19.345322465469515	12.770422268176402	24.638956119502538
2	16.626104495081975	21.26728850822278	36.98374021214128	25.12286678455397
3	17.978362923512496	26.33537817743492	29.69639512422009	25.989863774832493
4	13.08302986690683	17.382850692283366	37.07316827888443	32.460951161925365
5	14.437322731217995	37.24430204742737	33.10741122837541	15.210963992979226
6	31.014746192982027	37.24915583894071	17.76093219125704	13.975165776820228
7	27.39216559377923	32.05785016151975	22.305089632007615	18.244894612693408
8	27.19346445615725	31.856697734030444	20.44314990887627	20.506687900936043
9	27.40347225558894	14.408605019124252	20.068214298883607	38.1197084264032
10-11	25.466778667101707	25.144734350015703	27.19354476907313	22.194942213809462
12-13	26.398761169780666	23.46856887691536	26.81722638018095	23.315443573123023
14-15	24.402186709625102	24.40161404219644	24.579092058838224	26.617107189340228
16-17	23.832791548505895	26.570395536098097	25.347620529227573	24.249192386168442
18-19	24.091604925846585	25.784957667403702	27.351745816476765	22.771691590272948
20-21	25.612347579311567	24.957107833721032	26.074417157592965	23.35612742937444
22-23	24.77447998220925	24.746670582289784	26.051138210237735	24.42771122526323
24-25	24.19478863247448	25.49824179132943	26.267263785791606	24.03970579040448
26-27	24.373085405665208	25.414607258849315	26.10622597260519	24.106081362880293
28-29	23.88769729996372	25.322697594294624	26.71177528001175	24.0778298257299
30-31	24.48583824329174	25.115603664004603	26.375445429966536	24.023112662737116
32-33	24.07624229634883	25.290060759228844	25.84152008863599	24.792176855786334
34-35	24.593130481759125	25.20521173935769	26.429203063536637	23.772454715346544
36-37	24.488956684825293	25.18964538967446	26.02031874959821	24.30107917590204
38-39	24.586221567696885	24.831541620885204	26.234591895458692	24.347644915959226
40-41	24.38536534260134	25.226220494795882	26.37449734067536	24.013916821927413
42-43	24.900831591687233	25.046622942886344	26.058393469514495	23.99415199591193
44-45	24.37488183203009	25.40761573963123	25.70257976137092	24.514922666967763
46-47	24.139853870240554	25.24078250607063	25.952423514085034	24.666940109603782
48-49	24.90858826977403	25.436165838837155	25.445555552838623	24.209690338550192
50-51	24.20737122829178	25.766669117722827	25.21757781040807	24.808381843577322
52-53	24.252744425913217	25.5035324999497	25.23939146152522	25.004331612611868
54-55	24.500497024925597	25.4399057541095	25.333935728498158	24.725661492466745
56-57	24.536672311723688	25.097646141527193	24.990400887782073	25.375280658967046
58-59	24.15414987355318	25.113902750375132	25.656060243960216	25.07588713211147
60-61	24.301954763869016	25.313812186928867	25.432845811229015	24.9513872379731
62-63	23.996136784379477	25.28152223270111	25.20658772573265	25.51575325718677
64-65	23.962178512219097	25.09498091788771	25.419340848532762	25.52349972136043
66-67	24.46754371971236	25.156126547725517	25.2512564613258	25.125073271236325
68-69	24.469861837764793	24.863735843351865	25.239058698341456	25.427343620541894
70-71	23.927435940163193	24.997542374391227	25.47073748680795	25.604284198637632
72-73	24.62971982111882	24.977364251966815	25.355293306933895	25.037622619980464
74-75	24.72685353981177	25.000982500384357	25.349362290003864	24.922801669800013
76-77	24.233780887432037	25.301626607400983	25.362354205989707	25.102238299177277
78-79	24.263697186151557	25.376033210575734	25.3277380591155	25.03253154415721
80-81	23.988101588017503	25.162863155547388	25.674809108362428	25.17422614807268
82-83	24.31823121492385	25.189203016183644	25.314724241508856	25.17784152738365
84-85	24.34122770141275	25.32061115231949	24.98049564040231	25.357665505865455
86-87	23.938828564367036	25.06077548682911	25.249724379674387	25.750671569129462
88-89	24.082233104214914	24.74258189079053	25.378914056858825	25.79627094813573
90-91	24.282045797683192	24.874267816710354	25.239708080878216	25.603978304728237
92-93	24.095232284508032	25.00785882947393	25.149378877443084	25.747530008574955
94-95	23.51298917874025	25.097457693711377	25.56543133991887	25.824121787629505
96-97	24.065463938047998	25.01884836720824	25.12907132764169	25.786616367102074
98-99	23.658147904310145	25.155866963537495	25.241106272688402	25.94487885946396
100	23.696677598721287	25.20776793742315	24.959830670719892	26.135723793135675
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	451.0
1	391.5
2	516.0
3	713.0
4	613.5
5	572.0
6	850.5
7	1688.5
8	2685.5
9	2743.5
10	2056.0
11	1740.5
12	1904.5
13	2156.5
14	2501.5
15	3049.5
16	3790.0
17	4535.0
18	5365.0
19	6481.5
20	7871.5
21	9791.5
22	12617.5
23	16129.0
24	20443.0
25	27111.5
26	37687.0
27	49860.5
28	62177.5
29	79962.5
30	100543.0
31	120107.0
32	144461.5
33	169113.5
34	194182.5
35	214458.5
36	231885.5
37	260321.0
38	276531.0
39	282463.0
40	289414.5
41	296659.5
42	310359.5
43	345908.5
44	395254.0
45	436372.0
46	492771.5
47	549338.5
48	603288.0
49	642293.5
50	660549.0
51	643207.0
52	609816.5
53	610575.0
54	587873.5
55	551939.0
56	532672.0
57	490150.0
58	462039.5
59	438231.5
60	375145.0
61	302693.0
62	250770.0
63	198559.5
64	145641.5
65	120853.0
66	102855.5
67	79201.5
68	62017.5
69	52274.0
70	46732.0
71	42014.5
72	38326.5
73	41280.0
74	32493.5
75	22869.5
76	18617.5
77	15635.0
78	12620.0
79	8128.0
80	6216.0
81	5541.5
82	6004.5
83	6431.0
84	5156.5
85	3648.0
86	2706.5
87	1381.5
88	571.5
89	448.0
90	352.0
91	260.0
92	181.5
93	124.0
94	84.5
95	55.0
96	38.0
97	21.5
98	8.5
99	6.5
100	11.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.029680860216976166
2	0.0016202257812561107
3	3.6315405441947313E-4
4	0.0
5	0.0023744688173580933
6	2.9331673626188215E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	2.47922479459448E-4
16-17	0.004029613257692999
18-19	0.007029126072561532
20-21	0.0199595055294395
22-23	0.025759494802427434
24-25	0.03287242565677807
26-27	0.03213913381612337
28-29	0.04797474570835712
30-31	0.04775126629025283
32-33	0.05815004296391813
34-35	0.052332594361390795
36-37	0.05637268321680744
38-39	0.06080386105390659
40-41	0.04358896212806041
42-43	0.04958798775779748
44-45	0.0470563849745848
46-47	0.044964757295764955
48-49	0.0381102245185974
50-51	0.03795309055274282
52-53	0.039220637877303094
54-55	0.05825479894115452
56-57	0.04518823671386925
58-59	0.045069513273001335
60-61	0.04643832470889012
62-63	0.03137092331638987
64-65	0.03352889644745943
66-67	0.0340980705904438
68-69	0.031538532879968086
70-71	0.04467493242541095
72-73	0.03350445338610427
74-75	0.04204555739677765
76-77	0.0469202022041775
78-79	0.04163002535373998
80-81	0.03478596817429607
82-83	0.021555288249340457
84-85	0.03411203805407531
86-87	0.028004764581193987
88-89	0.02727496460644716
90-91	0.022669193473954033
92-93	0.016010205187627732
94-95	0.015832120026325874
96-97	0.014679804276725624
98-99	0.011551092423265548
100	0.005964106970658269
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.4318992E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.12252303851486
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.76478071028032	25.556826868893022
2	14.870854867998009	10.44603885407342
3	5.180364264618199	5.4584238969585535
4	2.318151247112023	3.2567728233381597
5	1.2235618984071603	2.148729048292723
6	0.736318517475047	1.5516818456221466
7	0.47137932966627627	1.1589221956258435
8	0.33851967460938714	0.9511732056367002
9	0.26243866201477195	0.8295757157529757
>10	1.5107442568342189	10.024617686130915
>50	0.14425294618380038	3.5549683997461057
>100	0.13173815216860604	9.861309369455224
>500	0.023974308533010535	5.968618054964312
>1k	0.020862861171765317	13.96993914449475
>5k	0.001878451215543266	4.372990987918089
>10k+	1.7985171212648291E-4	0.8894119030971663
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	22708	0.1585865820722576	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	20664	0.144311834240846	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18827	0.13148271889529656	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	6.983731815759098E-6	0.0	0.0
7	0.0	0.0	6.983731815759098E-6	0.0	6.983731815759098E-6
8	0.0	0.0	6.983731815759098E-6	0.0	6.983731815759098E-6
9	0.0	0.0	6.983731815759098E-6	1.3967463631518196E-5	6.983731815759098E-6
10-11	0.0	0.0	6.983731815759098E-6	2.793492726303639E-5	6.983731815759098E-6
12-13	1.3967463631518196E-5	6.983731815759098E-6	6.983731815759098E-6	4.1902390894554585E-5	6.983731815759098E-6
14-15	3.4918659078795493E-5	6.983731815759098E-6	6.983731815759098E-6	4.8886122710313684E-5	4.539425680243414E-5
16-17	4.1902390894554585E-5	6.983731815759098E-6	6.983731815759098E-6	9.428037951274783E-5	5.586985452607278E-5
18-19	4.5394256802434135E-5	6.983731815759098E-6	6.983731815759098E-6	1.3967463631518197E-4	7.682104997335009E-5
20-21	5.586985452607278E-5	1.3967463631518196E-5	1.0475597723638646E-5	3.3172726124855716E-4	7.682104997335009E-5
22-23	6.285358634183189E-5	1.3967463631518196E-5	1.3967463631518196E-5	9.567712587589964E-4	9.078851360486828E-5
24-25	6.285358634183189E-5	1.3967463631518196E-5	2.0951195447277293E-5	0.0025979482354623845	1.1173970905214557E-4
26-27	1.0126411132850692E-4	1.3967463631518196E-5	2.0951195447277293E-5	0.004528950082519775	1.1872344086790466E-4
28-29	1.1872344086790468E-4	1.3967463631518196E-5	2.7934927263036395E-5	0.015605148742313706	1.2570717268366378E-4
30-31	1.2919903859154333E-4	1.3967463631518196E-5	3.4918659078795493E-5	0.052028802027405285	1.2570717268366378E-4
32-33	1.3269090449942288E-4	2.0951195447277293E-5	3.4918659078795493E-5	0.11342278841974351	1.3269090449942288E-4
34-35	1.3269090449942288E-4	2.0951195447277293E-5	3.4918659078795493E-5	0.19142758093586476	1.3967463631518197E-4
36-37	1.3967463631518197E-4	2.0951195447277293E-5	3.4918659078795493E-5	0.31292705520053365	1.3967463631518197E-4
38-39	1.3967463631518197E-4	2.0951195447277293E-5	3.841052498667504E-5	0.49778992822958484	1.5364209994670017E-4
40-41	1.3967463631518197E-4	2.793492726303639E-5	4.1902390894554585E-5	0.7130040997299252	1.6062583176245927E-4
42-43	1.4665836813094107E-4	2.793492726303639E-5	4.5394256802434135E-5	0.9109859129748799	1.6760956357821837E-4
44-45	1.5364209994670017E-4	2.793492726303639E-5	4.8886122710313684E-5	1.1435860848305524	1.7459329539397744E-4
46-47	1.5364209994670017E-4	2.793492726303639E-5	5.586985452607278E-5	1.4008702567890254	1.78085161301857E-4
48-49	1.5364209994670017E-4	2.793492726303639E-5	5.936172043395234E-5	1.6594813377924926	2.0951195447277293E-4
50-51	1.5713396585457972E-4	3.4918659078795493E-5	6.285358634183189E-5	1.9266614577338963	2.0951195447277293E-4
52-53	1.6062583176245927E-4	4.8886122710313684E-5	6.285358634183189E-5	2.189295168263241	2.618899430909662E-4
54-55	1.6760956357821834E-4	4.8886122710313684E-5	6.285358634183189E-5	2.4561086422843172	3.1775979761703896E-4
56-57	1.6760956357821834E-4	4.8886122710313684E-5	6.285358634183189E-5	2.7612732795716344	3.2125166352491854E-4
58-59	1.6760956357821834E-4	4.8886122710313684E-5	6.285358634183189E-5	3.0931506910542304	3.247435294327981E-4
60-61	1.8157702720973654E-4	4.8886122710313684E-5	6.634545224971144E-5	3.4282441110379835	3.352191271564367E-4
62-63	1.8157702720973654E-4	4.8886122710313684E-5	6.983731815759099E-5	3.763693701344341	3.422028589721958E-4
64-65	1.8157702720973654E-4	5.2377988618193233E-5	6.983731815759099E-5	4.112227313207521	3.422028589721958E-4
66-67	1.850688931176161E-4	5.936172043395234E-5	6.983731815759099E-5	4.492872123959564	3.491865907879549E-4
68-69	1.8856075902549564E-4	6.285358634183189E-5	6.983731815759099E-5	4.9062077833411735	3.56170322603714E-4
70-71	1.8856075902549564E-4	6.285358634183189E-5	6.983731815759099E-5	5.362182617323901	3.771215180509913E-4
72-73	1.920526249333752E-4	6.285358634183189E-5	6.983731815759099E-5	5.800865731330809	4.1902390894554587E-4
74-75	2.0252822265701383E-4	6.285358634183189E-5	6.983731815759099E-5	6.245656118810597	4.1902390894554587E-4
76-77	2.0252822265701383E-4	6.983731815759099E-5	6.983731815759099E-5	6.729548420726822	4.225157748534254E-4
78-79	2.0252822265701383E-4	6.983731815759099E-5	6.983731815759099E-5	7.235373132410437	4.2600764076130497E-4
80-81	2.0252822265701383E-4	6.983731815759099E-5	6.983731815759099E-5	7.781382236961932	4.2949950666918454E-4
82-83	2.0252822265701383E-4	6.983731815759099E-5	6.983731815759099E-5	8.353772388447455	4.4695883620858226E-4
84-85	2.0252822265701383E-4	6.983731815759099E-5	8.380478178910917E-5	8.917390972772385	4.504507021164618E-4
86-87	2.0252822265701383E-4	7.682104997335009E-5	9.777224542062737E-5	9.511015160843723	4.5394256802434136E-4
88	2.0252822265701383E-4	7.682104997335009E-5	1.0475597723638647E-4	9.974256567780749	4.6092629984010046E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	61680	0.0	23.326988	1
CATGGGG	35630	0.0	20.690926	4
GTACATG	98675	0.0	20.682205	1
TACATGG	97395	0.0	20.393875	2
GTATAAG	5590	0.0	19.65705	1
TAGTACT	6030	0.0	19.390013	4
CAACGCA	67935	0.0	19.374279	5
AACGCAG	69910	0.0	19.196365	6
ATCAACG	69280	0.0	19.045593	3
ACATGGG	95980	0.0	19.02136	3
GTACAAG	6425	0.0	18.56414	1
TATCAAC	77345	0.0	18.522774	2
GTACATA	3655	0.0	18.500751	1
TATTCGC	1175	0.0	17.983606	9
TCAACGC	74130	0.0	17.831194	4
ACGCAGA	73915	0.0	17.641657	7
CGCAGAG	73935	0.0	17.541618	8
GTACTAG	5085	0.0	17.176561	1
CATGGGT	12770	0.0	16.804337	4
CTAGTAC	7495	0.0	16.790329	3
>>END_MODULE