FastQCFastQC Report
Fri 27 May 2016
ERR765561_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765561_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14193622
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT732730.5162389135063622No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA711400.5012110369009404No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG284760.20062532312048326No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC206930.14579083478480687No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC203810.1435926643671362No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191020.13458157473828739No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG185000.13034023309906378No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG183280.12912842120214277No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT167820.11823620496586423No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG48000.020.66481
GTATAAG57400.018.5091551
GTACATA36350.018.4936081
TAGTACT62000.018.0413134
CATGGGG332650.017.8442674
TCTAACG24000.017.6263792
CTAACGC24700.016.7447763
CTAGTAC65100.016.4606693
GTACAAG71900.015.9533161
GTATTAG45950.015.6529411
CGTTAAC15650.015.6199281
GTACATG1565950.015.2952411
GTCCTAC49600.015.1645091
TAGGCAT72750.015.1152465
TACATGG1554300.015.1144822
CTAGGCA78150.014.9745314
CTATTGA60250.014.8154789
ATACGGG39900.014.7241673
ATTGCGT30750.014.6700796
GTATATA36100.014.5848131
Produced by FastQC (version 0.11.5)