##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765561_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14193622
Sequences flagged as poor quality	0
Sequence length	100
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.759290334771492	33.0	33.0	33.0	27.0	33.0
2	31.68217407790626	33.0	33.0	33.0	27.0	33.0
3	31.607037090321274	33.0	33.0	33.0	27.0	33.0
4	35.22777448913322	37.0	37.0	37.0	33.0	37.0
5	35.460037754986004	37.0	37.0	37.0	33.0	37.0
6	35.44225406312779	37.0	37.0	37.0	33.0	37.0
7	35.306451446995	37.0	37.0	37.0	33.0	37.0
8	35.29467171945258	37.0	37.0	37.0	33.0	37.0
9	34.98531199435916	37.0	37.0	37.0	33.0	37.0
10-11	34.994400935856966	37.0	37.0	37.0	33.0	37.0
12-13	34.97528319409943	37.0	37.0	37.0	33.0	37.0
14-15	36.2880654423515	37.0	37.0	40.0	30.0	40.0
16-17	36.532964735851074	37.0	37.0	40.0	33.0	40.0
18-19	36.34389773801219	37.0	37.0	40.0	33.0	40.0
20-21	36.34386603363116	37.0	37.0	40.0	33.0	40.0
22-23	36.53725218975114	37.0	37.0	40.0	33.0	40.0
24-25	36.555639744386596	37.0	37.0	40.0	33.0	40.0
26-27	36.221811634831475	37.0	37.0	40.0	30.0	40.0
28-29	36.110652869295805	37.0	37.0	40.0	27.0	40.0
30-31	35.91768341442375	37.0	37.0	40.0	27.0	40.0
32-33	35.87357525795741	37.0	37.0	40.0	27.0	40.0
34-35	35.829016793599266	37.0	37.0	40.0	27.0	40.0
36-37	35.6059893662097	37.0	37.0	40.0	27.0	40.0
38-39	35.47423117228288	37.0	37.0	40.0	27.0	40.0
40-41	35.620526635132315	37.0	37.0	40.0	27.0	40.0
42-43	35.55184201749208	37.0	35.0	40.0	27.0	40.0
44-45	35.49917779971878	37.0	33.0	40.0	27.0	40.0
46-47	35.46110210628407	37.0	33.0	40.0	27.0	40.0
48-49	35.29877754247647	37.0	33.0	40.0	27.0	40.0
50-51	35.1575038774458	37.0	33.0	40.0	27.0	40.0
52-53	35.04962795965681	37.0	33.0	40.0	27.0	40.0
54-55	34.92891768570418	37.0	33.0	40.0	27.0	40.0
56-57	34.723681946722266	37.0	33.0	40.0	27.0	40.0
58-59	34.532588757119214	37.0	33.0	40.0	27.0	40.0
60-61	34.25059378782949	37.0	33.0	37.0	27.0	40.0
62-63	34.00899234881696	37.0	33.0	37.0	27.0	40.0
64-65	33.81519963685098	37.0	33.0	37.0	27.0	40.0
66-67	33.595156965572286	37.0	33.0	37.0	27.0	40.0
68-69	33.381155493643554	37.0	33.0	37.0	27.0	40.0
70-71	33.13136298120381	37.0	33.0	37.0	27.0	38.5
72-73	32.876789412878544	37.0	33.0	37.0	22.0	37.0
74-75	32.6482248505702	37.0	33.0	37.0	22.0	37.0
76-77	32.42980572541667	33.0	33.0	37.0	22.0	37.0
78-79	32.22662643826925	33.0	33.0	37.0	22.0	37.0
80-81	32.08687588693006	33.0	33.0	37.0	22.0	37.0
82-83	31.946213975544794	33.0	33.0	37.0	22.0	37.0
84-85	31.77217073274179	33.0	33.0	37.0	22.0	37.0
86-87	31.557359953646788	33.0	33.0	37.0	22.0	37.0
88-89	31.40667896467864	33.0	33.0	37.0	22.0	37.0
90-91	31.30936655914889	33.0	33.0	37.0	22.0	37.0
92-93	31.19362006399776	33.0	30.0	37.0	22.0	37.0
94-95	31.069239831806144	33.0	27.0	37.0	18.5	37.0
96-97	30.96951514560554	33.0	27.0	37.0	15.0	37.0
98-99	30.83325612729436	33.0	27.0	37.0	15.0	37.0
100	30.73101538141568	33.0	27.0	37.0	15.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	15.0
7	266.0
8	1945.0
9	4189.0
10	6132.0
11	9095.0
12	13798.0
13	22045.0
14	32000.0
15	39120.0
16	44747.0
17	52710.0
18	63508.0
19	75313.0
20	89057.0
21	105422.0
22	122259.0
23	130469.0
24	136223.0
25	150002.0
26	175250.0
27	212539.0
28	263046.0
29	328603.0
30	411343.0
31	512353.0
32	663054.0
33	879993.0
34	1169628.0
35	1588863.0
36	2364278.0
37	3293030.0
38	1232202.0
39	1125.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.431499664260684	18.34471707883255	12.641582529769991	25.582200727136772
2	15.513061318001336	20.648406668110393	37.83664915282175	26.001882861066516
3	18.10302509513013	26.05798840102316	29.146851266355768	26.692135237490945
4	12.602391817322406	17.34919891631458	36.41404280973175	33.63436645663126
5	14.050057087242115	37.25452186413539	33.555812564999584	15.139608483622904
6	32.05261785179783	36.015249798644675	17.451997658365638	14.480134691191859
7	27.46051933831497	31.616841463888285	22.19092358022473	18.73171561757201
8	27.369091152493887	32.80061580953589	20.06577324962833	19.764519788341893
9	27.266423269721233	14.297172648887107	19.534612902893976	38.90179117849769
10-11	25.14521879634331	25.265451374693786	27.004666877023777	22.584662951939134
12-13	26.603153972130283	22.882891883363968	26.50725400485316	24.006700139652587
14-15	24.44173361760378	23.567313473411012	24.48606794273196	27.504884966253247
16-17	23.765677455138963	26.193038804250108	25.66297509197289	24.378308648638043
18-19	24.30040132074609	25.549960342811158	27.282592329523936	22.867046006918816
20-21	25.184327787164662	24.98548869181913	26.421481104649114	23.408702416367092
22-23	24.565251889651567	24.93453586279055	25.800716596236935	24.69949565132095
24-25	24.187318685578777	25.5015510442192	25.845033422054538	24.46609684814749
26-27	24.55003506101321	25.001300252650715	25.99894993146365	24.449714754872424
28-29	24.110182880880128	25.014183546831926	26.606945282346857	24.26868828994109
30-31	24.710508162342247	25.05413379479012	26.28714942900926	23.94820861385838
32-33	24.002799577032686	25.329557334502407	25.96687695799031	24.700766130474598
34-35	24.0521845488262	25.44387965991876	26.558481279122287	23.94545451213275
36-37	24.709621994217144	25.03383881327213	26.11258557150924	24.143953621001486
38-39	24.81648456815188	24.729502779131252	26.11201906592316	24.341993586793713
40-41	24.23638428336626	25.150495254907828	26.309610510050398	24.30350995167551
42-43	25.007818802990165	24.917165452779805	25.86053271629322	24.214483027936804
44-45	24.712784810853847	25.186380265758668	25.636377370158225	24.46445755322926
46-47	24.21580337158973	25.161801734326218	26.320526620195757	24.301868273888296
48-49	24.928349561291867	25.226686107857223	25.74118745869778	24.103776872153126
50-51	23.937250779948023	25.688283956476553	25.533383241361513	24.84108202221391
52-53	23.837066939437808	25.690231786778867	24.97954797673188	25.493153297051446
54-55	24.140881728427036	25.662103725180224	24.960809862345215	25.236204684047525
56-57	24.523194291069608	24.98036794272808	24.95891464490177	25.537523121300538
58-59	24.12551919446636	24.93984269836128	25.79080237588404	25.143835731288323
60-61	24.36591590222707	25.325420812249334	25.336464504972728	24.97219878055087
62-63	23.756510792332488	25.344327201040418	25.31144606609665	25.587715940530448
64-65	23.474381563545222	25.318167245348988	25.526898696887713	25.680552494218077
66-67	24.491823568412443	25.09793959863303	25.209454887299493	25.200781945655038
68-69	24.5354737495261	24.768077701253375	25.185777738573435	25.510670810647092
70-71	23.789193565540433	25.057007173334394	25.35641658708566	25.797382674039515
72-73	24.561760354182777	25.002213149181472	25.276069444235528	25.159957052400223
74-75	24.765650713044437	24.904297606306724	25.177367661314925	25.15268401933391
76-77	24.11270088005114	25.143478327954266	25.702687836855	25.041132955139595
78-79	24.308382921340716	25.257185975107127	25.473057613209317	24.961373490342844
80-81	23.767155160964982	25.191031319364377	25.74684109721167	25.294972422458972
82-83	23.878277857639738	25.216702597796182	25.249044665483993	25.655974879080084
84-85	24.093531047401804	25.373267877678018	24.772532015878337	25.76066905904184
86-87	24.002089333706238	24.821818494647534	25.230892194601136	25.945199977045093
88-89	24.107060574487736	24.450995530547086	25.551301338186725	25.890642556778452
90-91	24.32204303683319	24.73493236485786	25.317965084055178	25.625059514253778
92-93	23.892812426537517	24.88401625176332	25.2769587243949	25.946212597304264
94-95	23.20900802414232	25.007813618495884	25.811492543454712	25.971685813907087
96-97	24.114065058376088	24.74153504529277	25.270155355688505	25.87424454064263
98-99	23.980580143773704	24.759742461758787	25.145175448825157	26.114501945642353
100	23.63525670283098	24.981008487032717	25.27951096519441	26.104223844941888
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1079.0
1	1032.0
2	1364.5
3	1716.5
4	1423.0
5	1251.5
6	1683.5
7	3064.5
8	5063.0
9	5139.5
10	3423.0
11	2408.0
12	2300.5
13	2484.5
14	2832.0
15	3436.5
16	4040.5
17	4793.0
18	5973.5
19	7392.5
20	9178.0
21	11492.5
22	14567.0
23	18310.0
24	22493.0
25	28184.5
26	36114.0
27	45023.5
28	54861.5
29	69292.0
30	85227.0
31	100343.5
32	119222.0
33	137806.5
34	157427.0
35	176129.5
36	194556.0
37	220452.0
38	240187.5
39	251960.0
40	262664.0
41	273329.0
42	290167.5
43	322390.5
44	365470.5
45	414480.5
46	474306.0
47	534082.0
48	598267.0
49	666092.5
50	782055.0
51	799027.0
52	693332.0
53	650018.0
54	627310.5
55	590537.0
56	554779.0
57	511945.5
58	477059.0
59	436743.0
60	371651.5
61	305043.5
62	247309.0
63	195976.0
64	149360.0
65	114972.5
66	89573.5
67	67600.0
68	52058.5
69	42100.5
70	34782.0
71	29676.5
72	25192.5
73	23387.5
74	19334.5
75	14703.5
76	10930.0
77	7394.5
78	5113.5
79	3172.5
80	2011.0
81	1409.5
82	972.5
83	609.0
84	354.0
85	214.5
86	162.0
87	109.5
88	61.0
89	45.0
90	30.0
91	18.0
92	14.5
93	10.0
94	9.0
95	6.5
96	2.0
97	1.0
98	1.0
99	2.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04945883439759069
2	0.024764644288822118
3	0.021558979096385687
4	0.022242384642905105
5	0.015394238341700237
6	0.024891461812918507
7	0.049268608111446115
8	0.006495875400937125
9	0.0043681591633199755
10-11	0.0020960118565930526
12-13	0.00131397045799867
14-15	0.002328510650769761
16-17	0.00696439569829322
18-19	0.0051783822339357775
20-21	0.008750409162650661
22-23	0.0186421760421688
24-25	0.025824979698628018
26-27	0.028688942117804744
28-29	0.045154083996318914
30-31	0.04289955023460537
32-33	0.04061331209186774
34-35	0.04857111172891599
36-37	0.0458727166328651
38-39	0.055616529734270795
40-41	0.05314711072339393
42-43	0.058240947941265456
44-45	0.05853685549749035
46-47	0.06325376285207539
48-49	0.06556113724882909
50-51	0.0342125498340029
52-53	2.712485932061598E-4
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	5.143155143909003E-4
64-65	1.7965815913654738E-4
66-67	3.69884445281127E-4
68-69	0.0012893114949799282
70-71	9.51131430722898E-5
72-73	3.2761193724899814E-4
74-75	8.10223070615802E-5
76-77	1.0568127008032198E-4
78-79	1.40908360107096E-5
80-81	4.9317926037483595E-5
82-83	2.3602150317938577E-4
84-85	0.0013809019290495408
86-87	0.004089865152108461
88-89	0.0027195313500669527
90-91	0.004181455586178074
92-93	0.004068728898092397
94-95	0.003198619774431079
96-97	0.0024905552648929216
98-99	0.0043892954173360395
100	0.0030999839223561122
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.4193622E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.580301016292
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.47653941629716	26.1463332745101
2	13.58771917461108	9.940857150642318
3	5.55463236698986	6.095703720579827
4	2.7686301030402998	4.051092902879268
5	1.6607370771090109	3.0375131094782275
6	1.004205973679307	2.204049407972859
7	0.6906954392316947	1.7686092954372785
8	0.48447021710906446	1.4177653100222332
9	0.40151013479102815	1.3218625432573006
>10	2.0473034566987183	13.672676336162
>50	0.1631999115195875	4.139197379828519
>100	0.12836757391226386	9.449569570675239
>500	0.01920298326116965	4.8896902191299825
>1k	0.011921704180920057	7.941692912280033
>5k	6.03116908403546E-4	1.4674891659871572
>10k+	2.613506603082033E-4	2.4558977011575016
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	73273	0.5162389135063622	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	71140	0.5012110369009404	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	28476	0.20062532312048326	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	20693	0.14579083478480687	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	20381	0.1435926643671362	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19102	0.13458157473828739	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	18500	0.13034023309906378	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	18328	0.12912842120214277	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	16782	0.11823620496586423	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.0454180053547996E-6
2	0.0	0.0	0.0	7.0454180053547996E-6	1.4090836010709599E-5
3	0.0	7.0454180053547996E-6	0.0	7.0454180053547996E-6	1.4090836010709599E-5
4	0.0	1.4090836010709599E-5	0.0	2.1136254016064397E-5	1.4090836010709599E-5
5	0.0	2.1136254016064397E-5	0.0	2.1136254016064397E-5	2.1136254016064397E-5
6	7.0454180053547996E-6	2.1136254016064397E-5	0.0	2.1136254016064397E-5	2.1136254016064397E-5
7	7.0454180053547996E-6	2.1136254016064397E-5	0.0	4.2272508032128794E-5	2.1136254016064397E-5
8	7.0454180053547996E-6	2.1136254016064397E-5	0.0	4.2272508032128794E-5	2.8181672021419198E-5
9	7.0454180053547996E-6	2.1136254016064397E-5	0.0	7.0454180053548E-5	2.8181672021419198E-5
10-11	7.0454180053547996E-6	2.4658963018741798E-5	0.0	8.454501606425759E-5	2.8181672021419198E-5
12-13	1.0568127008032198E-5	2.8181672021419198E-5	0.0	1.0568127008032199E-4	6.34087620481932E-5
14-15	1.4090836010709599E-5	3.5227090026774E-5	0.0	1.0568127008032199E-4	9.159043406961239E-5
16-17	1.4090836010709599E-5	3.5227090026774E-5	0.0	1.268175240963864E-4	1.092039790829994E-4
18-19	1.4090836010709599E-5	4.2272508032128794E-5	0.0	1.549991961178056E-4	1.23294815093709E-4
20-21	2.8181672021419198E-5	4.9317926037483595E-5	0.0	2.2545337617135359E-4	1.40908360107096E-4
22-23	2.8181672021419198E-5	5.6363344042838396E-5	0.0	9.934039387550267E-4	1.479537781124508E-4
24-25	3.17043810240966E-5	5.6363344042838396E-5	0.0	0.00254691860893576	1.549991961178056E-4
26-27	4.9317926037483595E-5	5.6363344042838396E-5	0.0	0.00457952170348062	1.549991961178056E-4
28-29	6.693147105087059E-5	5.6363344042838396E-5	0.0	0.014629810488119243	1.549991961178056E-4
30-31	9.51131430722898E-5	5.6363344042838396E-5	0.0	0.045657831383701775	1.549991961178056E-4
32-33	9.863585207496719E-5	5.6363344042838396E-5	0.0	0.10404321039407699	1.549991961178056E-4
34-35	9.863585207496719E-5	5.98860530455158E-5	0.0	0.1818211024641913	1.8318086813922478E-4
36-37	9.863585207496719E-5	6.34087620481932E-5	0.0	0.30309740529936613	1.8318086813922478E-4
38-39	9.863585207496719E-5	6.34087620481932E-5	0.0	0.4957966331638253	2.1488524916332138E-4
40-41	1.0568127008032199E-4	7.0454180053548E-5	0.0	0.7390009399996702	2.1840795816599877E-4
42-43	1.1272668808567679E-4	7.39768890562254E-5	0.0	0.9503317757792902	2.1840795816599877E-4
44-45	1.197721060910316E-4	7.74995980589028E-5	0.0	1.1937263089012797	2.2545337617135359E-4
46-47	1.197721060910316E-4	7.74995980589028E-5	0.0	1.4680572724847822	2.3249879417670837E-4
48-49	1.197721060910316E-4	7.74995980589028E-5	0.0	1.7520404587356206	2.3249879417670837E-4
50-51	1.197721060910316E-4	1.0568127008032199E-4	0.0	2.061986010336192	2.46589630187418E-4
52-53	1.197721060910316E-4	1.0568127008032199E-4	0.0	2.373023601727593	2.606804661981276E-4
54-55	1.197721060910316E-4	1.0568127008032199E-4	0.0	2.677311682669864	2.7477130220883715E-4
56-57	1.197721060910316E-4	1.0568127008032199E-4	0.0	3.0189897969665527	2.7477130220883715E-4
58-59	1.197721060910316E-4	1.0568127008032199E-4	0.0	3.3965044299474796	2.7477130220883715E-4
60-61	1.197721060910316E-4	1.0568127008032199E-4	0.0	3.778186427678573	2.7477130220883715E-4
62-63	1.197721060910316E-4	1.0568127008032199E-4	0.0	4.160763193496346	2.888621382195468E-4
64-65	1.197721060910316E-4	1.268175240963864E-4	0.0	4.563232697052239	2.9590755622490157E-4
66-67	1.268175240963864E-4	1.268175240963864E-4	0.0	5.001299175080187	2.9590755622490157E-4
68-69	1.268175240963864E-4	1.373856511044186E-4	0.0	5.479038401896288	2.9590755622490157E-4
70-71	1.303402330990638E-4	1.4795377811245079E-4	0.0	6.023913416885415	3.099983922356112E-4
72-73	1.3386294210174118E-4	1.4795377811245079E-4	0.0	6.544224581998872	3.4170277325970777E-4
74-75	1.3386294210174118E-4	1.4795377811245079E-4	0.0	7.053051715763601	3.7340715428380434E-4
76-77	1.3386294210174118E-4	1.4795377811245079E-4	0.0	7.592515849724616	3.87497990294514E-4
78-79	1.3386294210174118E-4	1.4795377811245079E-4	0.0	8.156381788947176	4.0511153530790097E-4
80-81	1.3386294210174118E-4	1.4795377811245079E-4	0.0	8.763228300711404	4.086342443105784E-4
82-83	1.373856511044186E-4	1.4795377811245079E-4	0.0	9.414327082967265	4.2272508032128797E-4
84-85	1.4795377811245079E-4	1.4795377811245079E-4	0.0	10.039995428932798	4.2272508032128797E-4
86-87	1.4795377811245079E-4	1.4795377811245079E-4	0.0	10.684538449734678	4.368159163319976E-4
88	1.4795377811245079E-4	1.549991961178056E-4	0.0	11.199854413482337	4.5090675234270717E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	4800	0.0	20.6648	1
GTATAAG	5740	0.0	18.509155	1
GTACATA	3635	0.0	18.493608	1
TAGTACT	6200	0.0	18.041313	4
CATGGGG	33265	0.0	17.844267	4
TCTAACG	2400	0.0	17.626379	2
CTAACGC	2470	0.0	16.744776	3
CTAGTAC	6510	0.0	16.460669	3
GTACAAG	7190	0.0	15.953316	1
GTATTAG	4595	0.0	15.652941	1
CGTTAAC	1565	0.0	15.619928	1
GTACATG	156595	0.0	15.295241	1
GTCCTAC	4960	0.0	15.164509	1
TAGGCAT	7275	0.0	15.115246	5
TACATGG	155430	0.0	15.114482	2
CTAGGCA	7815	0.0	14.974531	4
CTATTGA	6025	0.0	14.815478	9
ATACGGG	3990	0.0	14.724167	3
ATTGCGT	3075	0.0	14.670079	6
GTATATA	3610	0.0	14.584813	1
>>END_MODULE