FastQCFastQC Report
Fri 27 May 2016
ERR765560_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765560_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11341720
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT423800.37366466461877035No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA417070.36773082036939725No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG307980.2715461146986524No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG217060.19138190680073217No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT150950.13309268788155587No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG149020.13139100594971484No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145670.1284373093322706No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC138300.12193917677389321No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC131640.11606705155831744No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC127360.11229337349185133No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTC79300.021.1635655
GTATAAG42500.020.346811
TCTACAC91850.020.1092593
CTACACT115000.019.8222714
TGATCGC29600.019.0599449
CATGGGG252850.018.7558924
GTGATCG30850.018.1352658
ATCTACA89550.017.5800382
TAGTACT43700.016.8860384
CGTTAAC12950.016.693811
GTACTAG42700.016.069131
CTAGTAC53550.015.9732993
TAGGCAT59150.015.6568825
ATAAGGT41150.015.6470843
ATGGGGG147350.015.505295
TAAGGTG43750.015.3626984
GTATTAG35750.015.2492791
CTAACGC20200.015.1232353
ATACGGG28700.015.0656773
CACTCTT107300.014.9850617
Produced by FastQC (version 0.11.5)