##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765560_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11341720
Sequences flagged as poor quality	0
Sequence length	100
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.87205785365888	33.0	33.0	33.0	27.0	33.0
2	31.813786180579314	33.0	33.0	33.0	27.0	33.0
3	31.81410808942559	33.0	33.0	33.0	27.0	33.0
4	35.537992209294536	37.0	37.0	37.0	33.0	37.0
5	35.55255031864655	37.0	37.0	37.0	33.0	37.0
6	35.54646570361462	37.0	37.0	37.0	33.0	37.0
7	35.463806195180275	37.0	37.0	37.0	33.0	37.0
8	35.43785783814095	37.0	37.0	37.0	33.0	37.0
9	35.44278813090078	37.0	37.0	37.0	33.0	37.0
10-11	35.391560583403574	37.0	37.0	37.0	33.0	37.0
12-13	35.381406127112996	37.0	37.0	37.0	33.0	37.0
14-15	36.89277755049498	37.0	37.0	40.0	33.0	40.0
16-17	36.92292606412431	37.0	37.0	40.0	33.0	40.0
18-19	36.94888138659745	38.5	37.0	40.0	33.0	40.0
20-21	36.946937810138145	38.5	37.0	40.0	33.0	40.0
22-23	36.91791791721185	37.0	37.0	40.0	33.0	40.0
24-25	36.8760765122045	37.0	37.0	40.0	33.0	40.0
26-27	36.858630392921	37.0	37.0	40.0	33.0	40.0
28-29	36.824893711006794	37.0	37.0	40.0	33.0	40.0
30-31	36.7370356083557	37.0	37.0	40.0	33.0	40.0
32-33	36.63117825162321	37.0	37.0	40.0	33.0	40.0
34-35	36.519159175151565	37.0	37.0	40.0	33.0	40.0
36-37	36.45186246001488	37.0	37.0	40.0	33.0	40.0
38-39	36.351515290449775	37.0	37.0	40.0	33.0	40.0
40-41	36.19530009557633	37.0	37.0	40.0	33.0	40.0
42-43	36.08492486148485	37.0	37.0	40.0	33.0	40.0
44-45	35.943832901887895	37.0	37.0	40.0	27.0	40.0
46-47	35.72986385662845	37.0	37.0	40.0	27.0	40.0
48-49	35.50290965567832	37.0	35.0	40.0	27.0	40.0
50-51	35.2959320543974	37.0	33.0	40.0	27.0	40.0
52-53	35.12117346398959	37.0	33.0	40.0	27.0	40.0
54-55	34.985260128093444	37.0	33.0	40.0	27.0	40.0
56-57	34.82435428665141	37.0	33.0	40.0	27.0	40.0
58-59	34.64967509337208	37.0	33.0	38.5	27.0	40.0
60-61	34.370570513114416	37.0	33.0	37.0	27.0	40.0
62-63	34.15058862324233	37.0	33.0	37.0	27.0	40.0
64-65	33.92427568305337	37.0	33.0	37.0	27.0	40.0
66-67	33.694125229682975	37.0	33.0	37.0	27.0	40.0
68-69	33.46042725441996	37.0	33.0	37.0	27.0	40.0
70-71	33.22855029924914	37.0	33.0	37.0	27.0	37.0
72-73	32.96956788741038	37.0	33.0	37.0	27.0	37.0
74-75	32.785186109337914	37.0	33.0	37.0	24.5	37.0
76-77	32.577028131535606	33.0	33.0	37.0	22.0	37.0
78-79	32.37413954849882	33.0	33.0	37.0	22.0	37.0
80-81	32.19465804128474	33.0	33.0	37.0	22.0	37.0
82-83	32.03073568206586	33.0	33.0	37.0	22.0	37.0
84-85	31.875093239826057	33.0	33.0	37.0	22.0	37.0
86-87	31.740451668706335	33.0	33.0	37.0	22.0	37.0
88-89	31.622938848781313	33.0	33.0	37.0	22.0	37.0
90-91	31.501333880575434	33.0	33.0	37.0	22.0	37.0
92-93	31.346460325241672	33.0	33.0	37.0	22.0	37.0
94-95	31.268140546583766	33.0	33.0	37.0	22.0	37.0
96-97	31.096406850107392	33.0	27.0	37.0	22.0	37.0
98-99	30.976928587551093	33.0	27.0	37.0	22.0	37.0
100	30.902114053247654	33.0	27.0	37.0	15.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	14.0
7	262.0
8	1670.0
9	2338.0
10	2755.0
11	5022.0
12	11499.0
13	20269.0
14	25141.0
15	27235.0
16	30600.0
17	35615.0
18	44050.0
19	52504.0
20	63950.0
21	78926.0
22	93236.0
23	96263.0
24	97503.0
25	100772.0
26	111614.0
27	131403.0
28	161166.0
29	205045.0
30	265359.0
31	351214.0
32	476399.0
33	657690.0
34	939255.0
35	1421537.0
36	2239296.0
37	2765344.0
38	826390.0
39	384.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.15208027752555	19.022804704162528	12.793569204653929	25.031545813657992
2	16.00046909319524	21.0374101823203	37.38665712020866	25.5754636042758
3	18.27390563946717	25.90570930533599	29.371622284159503	26.448762771037337
4	13.174695257955513	17.311677797855978	36.20060107628685	33.31302586790166
5	14.099819098331057	37.21322367832218	33.43837064163878	15.248586581707988
6	31.723340209319918	36.77402481880215	17.248215708816517	14.254419263061422
7	27.09596289374931	32.15741322147822	21.973043279897407	18.773580604875058
8	26.79263694058134	33.07022583966368	20.18455441381823	19.952582805936753
9	27.073080576309433	14.693296411945065	19.558160180557863	38.67546283118764
10-11	25.092723937989657	25.714371641529883	27.000626890903344	22.192277529577115
12-13	26.29063911609833	23.52031240856432	26.781478536838577	23.407569938498778
14-15	24.23026786720108	24.035165961176705	24.61409614577838	27.12047002584384
16-17	23.54799492224433	26.827021687504644	25.523935629209916	24.10104776104111
18-19	24.070643087166893	26.146853852611766	27.197004799134273	22.585498261087068
20-21	25.145955249266187	25.34830961363983	26.13916689958727	23.366568237506712
22-23	24.565857880381582	25.309670110049137	25.64511345801445	24.47935855155483
24-25	24.254719608030896	25.744777804170987	25.645622521999677	24.354880065798444
26-27	24.464119314303026	25.45176733266526	25.67794472011981	24.406168632911903
28-29	24.091064252145944	25.22502497338443	26.582715653809796	24.101195120659835
30-31	24.80764857552063	25.119465998111863	26.170328459120906	23.902556967246603
32-33	23.885438871700046	25.628331526652744	25.93345800781629	24.552771593830926
34-35	24.055756152957535	25.604758621644397	26.31858096665972	24.020904258738344
36-37	24.516368619524325	25.516268141067872	25.771480775129348	24.195882464278455
38-39	24.756894758493818	24.872990971847383	25.91655189528953	24.453562374369262
40-41	24.23524398806281	25.1669926213547	26.18501974687527	24.412743643707216
42-43	24.888441008000047	25.11502420046928	25.849564631384258	24.146970160146417
44-45	24.587198488778082	25.210029091938956	25.902177231047695	24.300595188235267
46-47	24.14182698200587	25.221701383408167	26.251277087797153	24.38519454678881
48-49	24.82392916107351	25.281888831098097	25.91955698509862	23.97462502272977
50-51	24.18346358597898	25.6175366834454	25.44739503305949	24.751604697516132
52-53	23.906413576955067	25.750548558095392	25.118978086161253	25.224059778788288
54-55	24.223771833801173	25.50040075451916	25.07339522685845	25.202432184821213
56-57	24.3881050508984	24.923980222115404	25.208725358882223	25.479189368103977
58-59	24.113062111642957	24.79691264005147	26.003964706161415	25.08606054214416
60-61	24.254103744900203	25.22537822499611	25.512990983548338	25.00752704655535
62-63	23.57205083215704	25.29055809323238	25.486343150128832	25.651047924481745
64-65	23.414088368486755	25.136457421519292	25.615576158015408	25.833878051978548
66-67	24.153401489177913	25.08023437737343	25.287470050587107	25.478894082861554
68-69	24.127131154786486	24.625225589442316	25.454111795371226	25.793531460399972
70-71	23.583480353249875	24.79198208329922	25.66740473111612	25.95713283233478
72-73	24.24348266063074	24.723940529621327	25.64381323582002	25.388763573927918
74-75	24.18542518021315	24.652644217363147	25.726823009741818	25.43510759268188
76-77	23.683636782887284	24.83005156926601	25.905499920749154	25.58081172709755
78-79	23.82066897789357	24.860681406638356	25.877733810250557	25.440915805217518
80-81	23.549172276022592	24.77745137454773	25.994981813029135	25.67839453640055
82-83	23.653750928447636	24.75085773395999	25.777400755736977	25.817990581855398
84-85	23.78301065574956	24.79216068239687	25.432291319466856	25.99253734238672
86-87	23.53501411007592	24.487580351339588	25.81337332147056	26.16403221711393
88-89	23.68819191754517	24.03083821044671	26.05778701537061	26.22318285663751
90-91	23.80425600838401	24.206316054729367	25.905628130871776	26.083799806014845
92-93	23.483705344192852	24.38901102427909	25.87976275854383	26.247520872984232
94-95	22.914148054247637	24.420179454083126	26.208662830513735	26.4570096611555
96-97	23.44346641376905	24.335160645594296	25.824816613370306	26.396556327266353
98-99	23.256720097143273	24.332348295304023	25.77989966041031	26.631031947142393
100	23.246591392854736	24.29432123951018	25.801014302447513	26.658073065187573
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	826.0
1	759.5
2	686.5
3	640.0
4	521.0
5	455.0
6	598.5
7	1165.0
8	1830.0
9	1767.0
10	1253.0
11	1083.5
12	1224.0
13	1474.0
14	1799.5
15	2182.0
16	2663.0
17	3271.5
18	4035.0
19	4892.0
20	5936.0
21	7304.0
22	9216.5
23	11597.5
24	14181.5
25	17901.0
26	23693.0
27	30565.0
28	38356.5
29	51211.5
30	66720.5
31	81681.5
32	100656.0
33	119601.0
34	139507.0
35	157152.5
36	172703.0
37	198064.5
38	216409.0
39	224119.5
40	230333.5
41	237301.0
42	249806.5
43	277210.5
44	312884.5
45	347334.0
46	389516.0
47	433655.5
48	489543.0
49	538650.5
50	582520.5
51	588736.0
52	552218.5
53	527515.5
54	496808.0
55	456619.5
56	418534.5
57	375770.5
58	344190.0
59	317448.5
60	274312.5
61	229076.5
62	190053.0
63	152614.0
64	118162.0
65	93784.0
66	73896.5
67	56999.5
68	45623.0
69	37706.5
70	32068.0
71	27858.0
72	23958.0
73	22677.0
74	18834.0
75	14882.5
76	11858.5
77	8571.0
78	6451.5
79	4486.5
80	3236.0
81	2514.0
82	1880.5
83	1419.0
84	1038.0
85	694.0
86	463.0
87	273.5
88	138.5
89	86.0
90	65.5
91	47.5
92	36.0
93	22.5
94	15.5
95	11.5
96	6.5
97	3.5
98	4.0
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.023417964823677535
2	0.0060837333314523725
3	0.010721477871081282
4	0.00937247613236793
5	0.011506191300790357
6	0.007071237872209858
7	0.009672254296526453
8	0.009769241349636562
9	0.00765316019087052
10-11	0.028426023566090506
12-13	0.030775755352803632
14-15	0.013159379706076328
16-17	0.004311515360985812
18-19	0.01274498047915131
20-21	0.0020279111104841243
22-23	1.190295651805899E-4
24-25	0.0036855080181841904
26-27	0.0015826523666604359
28-29	0.0012828742025019133
30-31	0.026746384146319958
32-33	0.020490719220717844
34-35	0.00769724521501148
36-37	0.008645073234042103
38-39	0.006132226858007427
40-41	0.014971274198269751
42-43	0.003623788984386848
44-45	0.00382658009543526
46-47	0.012925729078129243
48-49	0.017334231492225165
50-51	0.007719287727081959
52-53	0.02157521081458544
54-55	0.027403251006020254
56-57	0.013922050623714922
58-59	0.010201274586217963
60-61	0.008380563089196349
62-63	0.015279869367256465
64-65	0.00884345584267642
66-67	0.017400359028436604
68-69	0.005268160384844627
70-71	0.005021284249655255
72-73	0.010434925214165046
74-75	0.0031608962309067757
76-77	0.010117513040350141
78-79	0.002371774298783606
80-81	0.006802319224950008
82-83	0.0018339370042639036
84-85	0.008711200770253542
86-87	0.0031432622212503925
88-89	0.00124319768077505
90-91	0.0011506191300790357
92-93	2.2924212553298795E-4
94-95	0.0028478925595059657
96-97	9.522365214447192E-4
98-99	0.004703872075840349
100	0.006383511495610895
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.134172E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.352817836706144
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.74554231740949	28.2834935076871
2	13.57515671748882	10.412910253811727
3	4.818533630687251	5.544130277333712
4	2.29762098467017	3.5248095633139367
5	1.280230893592097	2.455023112543061
6	0.798867409357684	1.8383289736065966
7	0.5767277703891786	1.5483394583374095
8	0.41176949205970315	1.2634016255743052
9	0.3187491857171298	1.1002436509866744
>10	1.8645373783570796	13.428077695590614
>50	0.15811729085269777	4.22411786094088
>100	0.12088114574731969	9.624507155570383
>500	0.02047269059610359	5.436487187471128
>1k	0.012215787852299882	8.38630666995309
>5k	2.771065071136984E-4	0.6770981184139234
>10k+	3.0019871603983994E-4	2.2527248888652847
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	42380	0.37366466461877035	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	41707	0.36773082036939725	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	30798	0.2715461146986524	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	21706	0.19138190680073217	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	15095	0.13309268788155587	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	14902	0.13139100594971484	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14567	0.1284373093322706	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	13830	0.12193917677389321	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	13164	0.11606705155831744	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	12736	0.11229337349185133	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.49667246237784E-4	0.0	0.0	2.645101448457553E-5	8.817004828191844E-6
2	7.847134297090741E-4	0.0	0.0	6.17190337973429E-5	8.817004828191844E-6
3	8.111644441936496E-4	0.0	0.0	6.17190337973429E-5	8.817004828191844E-6
4	9.346025117883354E-4	0.0	0.0	7.053603862553475E-5	8.817004828191844E-6
5	0.002936062607787884	0.0	0.0	1.410720772510695E-4	8.817004828191844E-6
6	0.006383511495610895	0.0	0.0	2.380591303611798E-4	2.645101448457553E-5
7	0.0074591860846503	0.0	0.0	2.468761351893716E-4	2.645101448457553E-5
8	0.009116782992350367	0.0	0.0	2.645101448457553E-4	2.645101448457553E-5
9	0.010095470528279662	0.0	0.0	2.7332714967394714E-4	2.645101448457553E-5
10-11	0.021332743181810165	0.0	0.0	5.378372945197025E-4	2.645101448457553E-5
12-13	0.040408333127603224	0.0	0.0	0.0011197596131803642	4.408502414095922E-5
14-15	0.0631297545698536	0.0	0.0	0.0017634009656383688	5.290202896915106E-5
16-17	0.07324285910778965	0.0	0.0	0.0020499536225546035	6.17190337973429E-5
18-19	0.07897391224611436	0.0	0.0	0.002283604250501688	6.612753621143883E-5
20-21	0.10015235784343116	0.0	0.0	0.0029140200957174043	7.935304345372659E-5
22-23	0.1061567381314298	0.0	0.0	0.003716367535082862	8.817004828191844E-5
24-25	0.13475469329167006	0.0	0.0	0.006555443089760636	8.817004828191844E-5
26-27	0.20806367993567115	0.0	0.0	0.014878695647573737	1.2784657000878175E-4
28-29	0.22266463993115682	0.0	0.0	0.029417936609262084	1.410720772510695E-4
30-31	0.2281003234077371	0.0	0.0	0.07399671302060006	1.4548057966516545E-4
32-33	0.23302021210186813	8.817004828191844E-6	0.0	0.1517538786004239	1.5870608690745318E-4
34-35	0.23669690311522415	8.817004828191844E-6	0.0	0.2588937127701971	1.5870608690745318E-4
36-37	0.24332288224361032	8.817004828191844E-6	0.0	0.42509425378161336	1.5870608690745318E-4
38-39	0.24954327914989965	8.817004828191844E-6	0.0	0.683639694861097	1.6752309173564503E-4
40-41	0.255027456153035	2.645101448457553E-5	0.0	1.0354910895349208	1.6752309173564503E-4
42-43	0.2699855048440625	2.645101448457553E-5	0.0	1.3093913445227003	1.7193159414974097E-4
44-45	0.28709931121558285	2.645101448457553E-5	0.0	1.593730933227059	1.7634009656383688E-4
46-47	0.29337701865325544	4.8493526555055144E-5	0.0	1.9277455271334505	1.8956560380612464E-4
48-49	0.298001537685642	5.290202896915106E-5	0.0	2.257558818239209	2.071996134625083E-4
50-51	0.3073607883107677	5.290202896915106E-5	0.0	2.6255012467244825	2.204251207047961E-4
52-53	0.3101601873437186	5.290202896915106E-5	0.0	3.025370049692639	2.512846376034676E-4
54-55	0.31929901284813944	6.17190337973429E-5	0.0	3.3883220534451564	2.556931400175635E-4
56-57	0.3399528466581788	7.053603862553475E-5	0.0	3.7726376598963824	2.556931400175635E-4
58-59	0.3457191678158163	7.053603862553475E-5	0.0	4.186688615130685	2.601016424316594E-4
60-61	0.34955897341849385	7.053603862553475E-5	0.0	4.589136392011088	2.645101448457553E-4
62-63	0.3527419121614711	7.053603862553475E-5	0.0	5.00292724560296	2.997781641585227E-4
64-65	0.35505196742645734	7.935304345372659E-5	0.0	5.43459457648399	3.041866665726186E-4
66-67	0.3584332887780689	8.817004828191844E-5	0.0	5.874787951033882	3.0859516898671454E-4
68-69	0.36130322384964536	8.817004828191844E-5	0.0	6.368430008852274	3.1741217381490636E-4
70-71	0.3653017355392304	9.257855069601436E-5	0.0	6.944511061814257	3.1741217381490636E-4
72-73	0.37667126326518374	9.698705311011029E-5	0.0	7.476701064741503	3.5708869554176967E-4
74-75	0.3878556338897451	9.698705311011029E-5	0.0	7.988519378013211	3.8794821244044115E-4
76-77	0.39126781475825534	9.698705311011029E-5	0.0	8.547989193878884	3.8794821244044115E-4
78-79	0.3935249679942725	1.0580405793830212E-4	0.0	9.12609815795135	3.8794821244044115E-4
80-81	0.3986873243211788	1.0580405793830212E-4	0.0	9.763378041425817	3.9235671485453706E-4
82-83	0.400807813982359	1.0580405793830212E-4	0.0	10.45744384449625	3.96765217268633E-4
84-85	0.40836839562253346	1.0580405793830212E-4	0.0	11.117493642939518	3.96765217268633E-4
86-87	0.42152777532860974	1.1462106276649397E-4	0.0	11.776767544957908	4.055822220968248E-4
88	0.4241684682746532	1.1462106276649397E-4	0.0	12.294228741319658	4.143992269250167E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACACTC	7930	0.0	21.163565	5
GTATAAG	4250	0.0	20.34681	1
TCTACAC	9185	0.0	20.109259	3
CTACACT	11500	0.0	19.822271	4
TGATCGC	2960	0.0	19.059944	9
CATGGGG	25285	0.0	18.755892	4
GTGATCG	3085	0.0	18.135265	8
ATCTACA	8955	0.0	17.580038	2
TAGTACT	4370	0.0	16.886038	4
CGTTAAC	1295	0.0	16.69381	1
GTACTAG	4270	0.0	16.06913	1
CTAGTAC	5355	0.0	15.973299	3
TAGGCAT	5915	0.0	15.656882	5
ATAAGGT	4115	0.0	15.647084	3
ATGGGGG	14735	0.0	15.50529	5
TAAGGTG	4375	0.0	15.362698	4
GTATTAG	3575	0.0	15.249279	1
CTAACGC	2020	0.0	15.123235	3
ATACGGG	2870	0.0	15.065677	3
CACTCTT	10730	0.0	14.985061	7
>>END_MODULE