FastQCFastQC Report
Fri 27 May 2016
ERR765558_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765558_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10071720
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3025383.0038364847315058No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2893612.873004809506221No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG1441441.431175608535583No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG1166821.1585111579750034No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG1156401.1481653580520506No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC966360.9594786193420786No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC929530.9229108831460763No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT824010.8181422835424337No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC817330.8115098513461455No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT811780.805999372500427No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG572480.5684034107381857No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA485230.4817747117672056No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC448070.44487932547767406No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC405300.4024138875981461No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT398270.39543394772690266No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC390510.3877292061336098No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA385900.38315203361491385No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC301330.2991842505550194No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG299610.2974764985523823No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT294350.292253954637341No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC277070.27509700428526607No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA268330.26641924120209853No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA257640.2558053639298948No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG245160.2434142331200629No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC242470.2407433884182642No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG241000.2392838561834523No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA231510.2298614337968093No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222510.2209255221551036No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA206540.20506924338643248No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG199280.19786094132878992No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT191550.19018598610763604No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC179670.17839058274058453No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA157780.1566564598698137No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC156840.1557231535427911No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC156720.15560400805423502No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG154110.15301259367814038No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG150520.1494481578121711No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC135350.1343861823005405No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG133370.13242028173936526No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG130140.12921328233906423No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126560.12565877526380798No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA114870.11405201892030357No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT113380.11257262910406565No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT112520.1117187531027471No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA111500.11070601645002044No Hit
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA109150.10837275063246396No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG104640.10389486602089813No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAA31100.032.4888421
GATCTAC134650.031.656081
ATCTACA151950.028.390312
TCTACAC178450.026.6519373
TACACTC181200.025.373565
CTACACT204000.025.160414
GTACTAG31100.023.1199681
TATTCGC5750.022.8944249
GTATAAG31600.021.7131081
TAGTACT34050.021.6724364
GTACATA23100.021.5650311
CACTCTT214600.021.5359157
ACACTCT215950.021.4005416
GAACAAA59700.021.0180841
ACTCTTT231050.020.9183148
CTATTGA36750.020.7251289
AGTACTC32400.020.6036435
GTACAAG33700.020.4995171
CGTTAAC10750.020.10971
TAGGCAT45450.019.8594885
Produced by FastQC (version 0.11.5)