FastQCFastQC Report
Fri 27 May 2016
ERR765557_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765557_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19834934
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT356170.17956702049021186No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT218390.11010371902422261No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA213320.10754762279521575No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199320.10048936890841179No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA696300.029.909821
CAACGCA809700.024.263195
ATCAACG820100.023.8471763
AACGCAG837350.023.5661226
TATCAAC901450.022.8472392
TCAACGC892250.022.0396184
ACGCAGA888750.021.7009857
CGCAGAG894300.021.4664888
GTGATCG55650.020.8534378
GTACATG1186000.020.7495331
GCAGAGT896150.020.5361379
TACATGG1164400.020.0969922
GTACTAG81150.020.0933071
GTATAAG85600.019.6525881
TGATCGC59200.019.5235719
GTACAAG96100.019.2167361
CTATTGA86650.019.1404449
GTACATA48300.019.0686261
CATGGGG522800.018.5510834
ACATGGG1180850.018.4878393
Produced by FastQC (version 0.11.5)