##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765557_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	19834934
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.875195803525234	33.0	33.0	33.0	27.0	33.0
2	31.770686355699496	33.0	33.0	33.0	27.0	33.0
3	31.803461408038967	33.0	33.0	33.0	27.0	33.0
4	35.54386150213558	37.0	37.0	37.0	33.0	37.0
5	35.6049859807953	37.0	37.0	37.0	33.0	37.0
6	35.593283748763675	37.0	37.0	37.0	33.0	37.0
7	35.51850966582495	37.0	37.0	37.0	33.0	37.0
8	35.50313300765205	37.0	37.0	37.0	33.0	37.0
9	35.46108174597405	37.0	37.0	37.0	33.0	37.0
10-11	35.45180856160147	37.0	37.0	37.0	33.0	37.0
12-13	35.40464127584191	37.0	37.0	37.0	33.0	37.0
14-15	36.9800221165344	40.0	37.0	40.0	33.0	40.0
16-17	37.036016958765785	40.0	37.0	40.0	33.0	40.0
18-19	37.02594117530212	40.0	37.0	40.0	33.0	40.0
20-21	37.124424865744444	40.0	37.0	40.0	33.0	40.0
22-23	37.11773238065728	40.0	37.0	40.0	33.0	40.0
24-25	37.10206270411588	40.0	37.0	40.0	33.0	40.0
26-27	37.06083914874635	40.0	37.0	40.0	33.0	40.0
28-29	37.01470030099419	40.0	37.0	40.0	33.0	40.0
30-31	36.92991108515915	38.5	37.0	40.0	33.0	40.0
32-33	36.8156185445336	37.0	37.0	40.0	33.0	40.0
34-35	36.695579929834906	37.0	37.0	40.0	33.0	40.0
36-37	36.59479476967254	37.0	37.0	40.0	33.0	40.0
38-39	36.47980270567071	37.0	37.0	40.0	33.0	40.0
40-41	36.36911738652621	37.0	37.0	40.0	33.0	40.0
42-43	36.214108577321205	37.0	37.0	40.0	33.0	40.0
44-45	36.049869059307184	37.0	37.0	40.0	27.0	40.0
46-47	35.84862535968105	37.0	37.0	40.0	27.0	40.0
48-49	35.58047266504643	37.0	37.0	40.0	27.0	40.0
50-51	35.46350285309747	37.0	33.0	40.0	27.0	40.0
52-53	35.29843827562017	37.0	33.0	40.0	27.0	40.0
54-55	35.13497750988231	37.0	33.0	40.0	27.0	40.0
56-57	34.967268784458774	37.0	33.0	40.0	27.0	40.0
58-59	34.793093891817335	37.0	33.0	40.0	27.0	40.0
60-61	34.60601333485657	37.0	33.0	38.5	27.0	40.0
62-63	34.3866804648808	37.0	33.0	37.0	27.0	40.0
64-65	34.1871624579139	37.0	33.0	37.0	27.0	40.0
66-67	33.99499458884007	37.0	33.0	37.0	27.0	40.0
68-69	33.80204821957058	37.0	33.0	37.0	27.0	40.0
70-71	33.62205593928369	37.0	33.0	37.0	27.0	40.0
72-73	33.424397958672316	37.0	33.0	37.0	27.0	37.0
74-75	33.2143768918011	37.0	33.0	37.0	27.0	37.0
76-77	33.03336199656626	37.0	33.0	37.0	27.0	37.0
78-79	32.88061737437594	37.0	33.0	37.0	24.5	37.0
80-81	32.72076534260209	37.0	33.0	37.0	22.0	37.0
82-83	32.594966058369536	37.0	33.0	37.0	22.0	37.0
84-85	32.4623318131535	35.0	33.0	37.0	22.0	37.0
86-87	32.35075720443537	33.0	33.0	37.0	22.0	37.0
88-89	32.23836832529919	33.0	33.0	37.0	22.0	37.0
90-91	32.123384630369834	33.0	33.0	37.0	22.0	37.0
92-93	32.028736722794235	33.0	33.0	37.0	22.0	37.0
94-95	31.92240317008365	33.0	33.0	37.0	22.0	37.0
96-97	31.84129153139607	33.0	33.0	37.0	22.0	37.0
98-99	31.73769685848211	33.0	33.0	37.0	22.0	37.0
100	31.68533658846558	33.0	33.0	37.0	22.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	4.0
6	19.0
7	234.0
8	1303.0
9	2618.0
10	4276.0
11	7321.0
12	15014.0
13	28800.0
14	45535.0
15	54537.0
16	60109.0
17	71540.0
18	86942.0
19	103579.0
20	120690.0
21	142840.0
22	167764.0
23	168923.0
24	161356.0
25	169649.0
26	191278.0
27	222625.0
28	273383.0
29	346265.0
30	443324.0
31	573356.0
32	751811.0
33	999273.0
34	1316707.0
35	1832608.0
36	3095332.0
37	5563785.0
38	2808080.0
39	4054.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.586558282041224	19.634196342366824	13.279989172698237	24.49925620289371
2	16.17995343594326	21.28526557732165	37.67682293915831	24.857958047576776
3	17.719086670247446	26.700964909719932	29.648941907823513	25.931006512209116
4	12.789703322964504	17.58713151327507	37.09017795439144	32.53298720936898
5	13.944970435660341	37.77816044523527	33.19265764718124	15.084211471923146
6	30.7450833967988	38.005692380927506	17.474628350162398	13.774595872111295
7	26.83256722709539	32.55137627380056	22.386971390981188	18.22908510812287
8	26.66629493196196	32.743219614443895	20.174692792020384	20.415792661573768
9	27.00869586962074	14.77644947041417	20.127891527140953	38.08696313282414
10-11	25.24426347977765	25.750710841790546	27.045915554848833	21.959110123582967
12-13	26.026512112417414	24.01900102112767	26.883706797310243	23.07078006914467
14-15	23.991269645767414	24.58831977963728	24.86113893799697	26.559271636598336
16-17	23.317022397822218	26.8576033020664	25.523381129252158	24.301993170859223
18-19	23.59856986308953	26.173942616299712	27.660581787753824	22.566905732856938
20-21	25.373524378206376	25.246880907620195	26.34091674102455	23.038677973148882
22-23	24.656748891946098	24.973747711976497	26.38790373220753	23.981599663869876
24-25	23.92275662573624	25.73047391448022	26.677268765654706	23.669500694128832
26-27	24.02742761456304	25.70204564615471	26.46219221711173	23.80833452217052
28-29	23.503132950280197	25.639041728748534	27.010051955382846	23.847773365588424
30-31	24.129592714382138	25.36966376389081	26.743407108294125	23.757336413432924
32-33	23.7652312798798	25.652168388480696	26.194705445811895	24.38789488582761
34-35	24.241522972773975	25.491804615719772	26.788510546045575	23.47816186546068
36-37	24.184869478472574	25.391593827518804	26.345462781795582	24.078073912213036
38-39	24.16204081283784	25.124470877902898	26.56493910609501	24.14854920316425
40-41	24.02201326998214	25.35990207695133	26.825078945452702	23.793005707613826
42-43	24.334878059265186	25.287755577218668	26.460110155796666	23.91725620771948
44-45	23.885943512407216	25.6924197975085	26.165126376039264	24.25651031404502
46-47	23.805849616185103	25.461779127255934	26.25122032041206	24.481150936146896
48-49	24.451663008712657	25.618198849581763	25.783479292232226	24.146658849473354
50-51	23.793001310871727	25.91655451974187	25.51201633258896	24.77842783679744
52-53	23.765426682247412	25.735962755484177	25.524612090085107	24.973998472183307
54-55	23.963964668148346	25.70054461722294	25.61709522436543	24.718395490263294
56-57	24.053726380929337	25.32357118036529	25.344917664766708	25.27778477393866
58-59	23.747873770031415	25.33647233920001	25.833818603006748	25.081835287761827
60-61	23.856165366498555	25.43795140850901	25.65438948809719	25.051493736895242
62-63	23.656635835366927	25.36074869715157	25.431567557367803	25.5510479101137
64-65	23.49594428939247	25.263266221531467	25.669888572330972	25.57090091674509
66-67	24.050110928708403	25.249616381582353	25.501357498530837	25.198915191178408
68-69	23.927690682697243	25.018291084554043	25.568412689825305	25.485605542923405
70-71	23.46135949781373	25.101490816017396	25.755586019067977	25.6815636671009
72-73	24.048600102456124	25.174903997805664	25.602504527834586	25.173991371903625
74-75	24.08229829153979	25.25003512230237	25.659089887557457	25.008576698600375
76-77	23.78058515495892	25.427107577616724	25.594057447807305	25.198249819617047
78-79	23.81187428797564	25.500771925094035	25.542390159209848	25.144963627720472
80-81	23.567939767070108	25.303484348910626	25.94972307295795	25.178852811061308
82-83	23.84849098704172	25.194072201877038	25.74020843880827	25.217228372272977
84-85	23.90035983637847	25.293913768085563	25.295227140742604	25.510499254793363
86-87	23.498602785112656	25.13936100497427	25.652684837669877	25.709351372243194
88-89	23.685578676317633	24.74189927167028	25.68904250520981	25.883479546802274
90-91	23.865974114995208	24.89997928132803	25.556767989014034	25.677278614662725
92-93	23.768807885615	24.982572208431293	25.475715562913255	25.772904343040448
94-95	23.214767338847036	25.03213564371329	25.807096797467032	25.946000219972642
96-97	23.629080288196537	24.987245810477507	25.426187059732158	25.957486841593795
98-99	23.291472503377626	25.194175473154264	25.45373740724687	26.060614616221244
100	23.407164179676375	25.2012064040187	25.16161374107911	26.230015675225815
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1544.0
1	1328.5
2	1551.0
3	2027.5
4	1751.5
5	1548.5
6	1975.5
7	3661.0
8	6051.0
9	5988.0
10	3934.0
11	2978.0
12	3094.0
13	3493.0
14	4214.5
15	5047.0
16	5952.5
17	7173.0
18	8766.0
19	10632.0
20	13182.0
21	16385.5
22	20418.5
23	26126.0
24	33073.0
25	42403.5
26	56798.5
27	73288.5
28	90586.5
29	116750.0
30	146549.0
31	173725.5
32	210040.0
33	248431.0
34	285818.5
35	315982.5
36	343335.0
37	382793.5
38	402013.5
39	408105.5
40	418071.5
41	428693.5
42	445969.5
43	489964.5
44	554325.5
45	616721.0
46	692750.0
47	766108.0
48	829056.0
49	865987.0
50	883666.0
51	872971.0
52	842430.5
53	839383.0
54	819331.0
55	779592.5
56	746709.5
57	687698.0
58	634262.5
59	587712.0
60	503389.0
61	403944.5
62	325373.0
63	256640.5
64	193276.5
65	158461.0
66	134549.5
67	104004.0
68	78658.0
69	61859.5
70	52442.5
71	45338.5
72	39852.5
73	41630.0
74	32893.5
75	23622.0
76	19532.0
77	15911.5
78	12774.5
79	8896.5
80	6640.0
81	5371.5
82	5118.0
83	4977.0
84	3798.0
85	2508.5
86	1812.5
87	954.5
88	419.0
89	284.5
90	239.5
91	187.0
92	125.5
93	86.0
94	62.5
95	50.5
96	34.0
97	20.0
98	17.5
99	13.5
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01807417156013728
2	0.005954141314511054
3	0.006377636547719291
4	0.004068579204750568
5	0.04633743676686799
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	4.1089120841037336E-4
18-19	0.0016612104683585032
20-21	5.923891654996179E-4
22-23	0.004781967008309683
24-25	0.007259918283569786
26-27	0.00568441518383676
28-29	0.021502466305156346
30-31	0.02003283701372538
32-33	0.012886354953336373
34-35	0.013322454211342472
36-37	0.013360266285736066
38-39	0.020622705374265426
40-41	0.02971776966840424
42-43	0.017527156883909975
44-45	0.016571771804231868
46-47	0.017970818556794794
48-49	0.013014916006274586
50-51	0.009823576927455367
52-53	0.026871780869046503
54-55	0.024963531514649858
56-57	0.013251871672474434
58-59	0.016019715518085415
60-61	0.017121307285418746
62-63	0.020461373856852764
64-65	0.010879794205516389
66-67	0.015389514278192204
68-69	0.007567456488637674
70-71	0.008749714014677336
72-73	0.010355466773925237
74-75	0.003380399450787182
76-77	0.007237231038933631
78-79	0.005442417907717767
80-81	0.0023594734421601804
82-83	2.4199727611899288E-4
84-85	0.002533408984370707
86-87	0.0023998063215133463
88-89	0.0011898199409183818
90-91	8.495112713760479E-4
92-93	0.0011847783309992359
94-95	0.0032745256424851227
96-97	0.0013864427277650634
98-99	0.0018225419857711652
100	0.002273766073534704
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.9834934E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.01224379882118
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.0783147147448	21.73285780944184
2	14.973948823859892	9.287515031132294
3	5.822274110846052	5.4168535256736785
4	2.821387948487374	3.4999028363818554
5	1.57887377146976	2.4482209164192237
6	0.991953316477661	1.8457618852592668
7	0.6330511320689443	1.374263523138929
8	0.45878822408997955	1.1382441806005321
9	0.33496634267472664	0.9349252095085293
>10	1.8816029811092099	10.911840230068877
>50	0.20197914754681517	4.3876200124251215
>100	0.17007359039296727	10.940416527118003
>500	0.026111529404188566	5.6271136164879945
>1k	0.02431801934461976	14.76112263795802
>5k	0.0020454405239656317	4.242552438340836
>10k+	3.10906959642776E-4	1.4507896200453658
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35617	0.17956702049021186	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	21839	0.11010371902422261	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	21332	0.10754762279521575	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19932	0.10048936890841179	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.008321983829137E-5	0.0
2	0.0	0.0	0.0	1.008321983829137E-5	0.0
3	0.0	0.0	0.0	1.008321983829137E-5	0.0
4	0.0	0.0	0.0	1.008321983829137E-5	0.0
5	0.0	0.0	0.0	1.008321983829137E-5	0.0
6	0.0	0.0	0.0	1.008321983829137E-5	0.0
7	0.0	0.0	0.0	1.008321983829137E-5	0.0
8	5.041609919145685E-6	0.0	0.0	1.008321983829137E-5	0.0
9	5.041609919145685E-6	0.0	0.0	1.008321983829137E-5	5.041609919145685E-6
10-11	5.041609919145685E-6	0.0	0.0	2.5208049595728427E-5	5.041609919145685E-6
12-13	7.562414878718528E-6	0.0	0.0	2.5208049595728427E-5	3.024965951487411E-5
14-15	1.008321983829137E-5	5.041609919145685E-6	0.0	4.0332879353165485E-5	3.52912694340198E-5
16-17	1.008321983829137E-5	5.041609919145685E-6	0.0	6.049931902974822E-5	3.52912694340198E-5
18-19	1.5124829757437055E-5	1.008321983829137E-5	0.0	8.31865636659038E-5	4.033287935316548E-5
20-21	5.5457709110602534E-5	1.008321983829137E-5	0.0	1.2856105293821497E-4	4.285368431273832E-5
22-23	6.049931902974822E-5	1.008321983829137E-5	0.0	6.377636547719291E-4	4.5374489272311166E-5
24-25	7.562414878718528E-5	1.008321983829137E-5	0.0	0.002223349974343247	4.5374489272311166E-5
26-27	1.4368588269565204E-4	1.008321983829137E-5	0.0	0.0038870812476613234	5.5457709110602534E-5
28-29	1.5628990749351623E-4	1.008321983829137E-5	0.0	0.01685158115474445	5.797851407017538E-5
30-31	1.5628990749351623E-4	1.008321983829137E-5	0.0	0.06431329693358193	6.302012398932106E-5
32-33	1.663731273318076E-4	1.008321983829137E-5	0.0	0.14728307137296248	6.554092894889391E-5
34-35	1.6889393229138045E-4	1.2604024797864212E-5	0.0	0.2549365679764803	6.554092894889391E-5
36-37	1.7393554221052613E-4	1.5124829757437055E-5	0.0	0.4277226231254412	6.554092894889391E-5
38-39	1.8149795708924466E-4	1.5124829757437055E-5	0.0	0.7041162829178056	7.05825388680396E-5
40-41	1.890603719679632E-4	1.5124829757437055E-5	0.0	1.0272255002209736	7.05825388680396E-5
42-43	2.0418520172540026E-4	1.5124829757437055E-5	0.0	1.3096136342072024	7.562414878718527E-5
44-45	2.067060066849731E-4	1.5124829757437055E-5	0.0	1.6385635566017007	8.066575870633096E-5
46-47	2.067060066849731E-4	1.5124829757437055E-5	0.0	2.00338957518084	9.074897854462233E-5
48-49	2.067060066849731E-4	1.5124829757437055E-5	0.0	2.3910692115234666	9.579058846376802E-5
50-51	2.067060066849731E-4	1.5124829757437055E-5	0.0	2.794632439916362	9.579058846376802E-5
52-53	2.067060066849731E-4	1.5124829757437055E-5	0.0	3.1784073493766103	1.008321983829137E-4
54-55	2.142684215636916E-4	1.5124829757437055E-5	0.0	3.562250320570767	1.0335300334248654E-4
56-57	2.1678922652326445E-4	1.5124829757437055E-5	0.0	3.99828151684296	1.0587380830205938E-4
58-59	2.193100314828373E-4	2.016643967658274E-5	0.0	4.48414146475103	1.0587380830205938E-4
60-61	2.2183083644241014E-4	2.016643967658274E-5	0.0	4.98917213437665	1.0587380830205938E-4
62-63	2.2183083644241014E-4	2.016643967658274E-5	0.0	5.497288269272789	1.1091541822120507E-4
64-65	2.2435164140198298E-4	2.016643967658274E-5	0.0	6.004184334568494	1.1091541822120507E-4
66-67	2.319140562807015E-4	2.016643967658274E-5	0.0	6.551758629496826	1.1091541822120507E-4
68-69	2.369556661998472E-4	2.016643967658274E-5	0.0	7.156356053415656	1.1091541822120507E-4
70-71	2.369556661998472E-4	2.2687244636155583E-5	0.0	7.824402138166933	1.1343622318077791E-4
72-73	2.369556661998472E-4	2.5208049595728427E-5	0.0	8.480512211434633	1.1595702814035076E-4
74-75	2.369556661998472E-4	2.7728854555301267E-5	0.0	9.126055070311804	1.1595702814035076E-4
76-77	2.369556661998472E-4	3.024965951487411E-5	0.0	9.814902837589479	1.1595702814035076E-4
78-79	2.4199727611899288E-4	3.024965951487411E-5	0.0	10.539886343962626	1.2604024797864213E-4
80-81	2.4451808107856573E-4	3.024965951487411E-5	0.0	11.333473557310551	1.3360266285736066E-4
82-83	2.4703888603813857E-4	3.52912694340198E-5	0.0	12.163902335142634	1.4620668765522488E-4
84-85	2.4703888603813857E-4	4.033287935316548E-5	0.0	12.963040361011537	1.4620668765522488E-4
86-87	2.4703888603813857E-4	4.789529423188401E-5	0.0	13.773406052170378	1.4872749261479772E-4
88	2.4703888603813857E-4	5.5457709110602534E-5	0.0	14.401101611933774	1.5124829757437054E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	69630	0.0	29.90982	1
CAACGCA	80970	0.0	24.26319	5
ATCAACG	82010	0.0	23.847176	3
AACGCAG	83735	0.0	23.566122	6
TATCAAC	90145	0.0	22.847239	2
TCAACGC	89225	0.0	22.039618	4
ACGCAGA	88875	0.0	21.700985	7
CGCAGAG	89430	0.0	21.466488	8
GTGATCG	5565	0.0	20.853437	8
GTACATG	118600	0.0	20.749533	1
GCAGAGT	89615	0.0	20.536137	9
TACATGG	116440	0.0	20.096992	2
GTACTAG	8115	0.0	20.093307	1
GTATAAG	8560	0.0	19.652588	1
TGATCGC	5920	0.0	19.523571	9
GTACAAG	9610	0.0	19.216736	1
CTATTGA	8665	0.0	19.140444	9
GTACATA	4830	0.0	19.068626	1
CATGGGG	52280	0.0	18.551083	4
ACATGGG	118085	0.0	18.487839	3
>>END_MODULE