##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765556_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7104683
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.33156876950034	33.0	31.0	34.0	31.0	34.0
2	32.337603943764975	34.0	31.0	34.0	31.0	34.0
3	32.37176127351495	34.0	31.0	34.0	31.0	34.0
4	35.82489831003016	37.0	35.0	37.0	35.0	37.0
5	35.84044523872494	37.0	35.0	37.0	35.0	37.0
6	35.951610930424344	37.0	37.0	37.0	35.0	37.0
7	35.90657148249964	37.0	37.0	37.0	35.0	37.0
8	35.94606867048115	37.0	37.0	37.0	35.0	37.0
9	37.65114150765066	39.0	38.0	39.0	35.0	39.0
10-11	37.583978764429034	39.0	38.0	39.0	35.0	39.0
12-13	37.55933586340164	39.0	38.0	39.0	35.0	39.0
14-15	38.91609133018321	41.0	39.0	41.0	36.0	41.0
16-17	38.86712433193712	41.0	39.0	41.0	36.0	41.0
18-19	38.87609651268044	41.0	39.0	41.0	35.5	41.0
20-21	38.858659281490816	41.0	39.0	41.0	35.0	41.0
22-23	38.77264319885912	41.0	39.0	41.0	35.0	41.0
24-25	38.695100752559966	41.0	39.0	41.0	35.0	41.0
26-27	38.54924491353098	40.5	38.0	41.0	35.0	41.0
28-29	38.37673939850659	40.0	38.0	41.0	34.0	41.0
30-31	38.09858166789426	40.0	38.0	41.0	34.0	41.0
32-33	37.76182061887913	40.0	37.0	41.0	33.5	41.0
34-35	37.61278356824646	40.0	37.0	41.0	33.0	41.0
36-37	37.44095204529182	40.0	37.0	41.0	33.0	41.0
38-39	37.204022966260425	40.0	37.0	41.0	32.5	41.0
40-41	36.901765075232774	40.0	36.0	41.0	31.0	41.0
42-43	36.57912576817291	40.0	36.0	41.0	31.0	41.0
44-45	36.099734499062095	40.0	35.0	41.0	29.5	41.0
46-47	35.59550876513421	39.0	35.0	41.0	27.5	41.0
48-49	35.15461639878936	39.0	35.0	41.0	25.0	41.0
50-51	33.88087406573946	38.0	34.0	40.0	23.0	40.5
52-53	33.800756768458214	38.0	34.0	39.5	22.0	40.0
54-55	34.54706128338168	39.0	35.0	40.5	22.0	41.0
56-57	34.5151006033626	39.0	35.0	41.0	21.5	41.0
58-59	34.29119553961802	38.0	35.0	41.0	20.0	41.0
60-61	33.89875269593309	38.0	35.0	40.0	18.0	41.0
62-63	33.48117382577097	37.0	34.0	40.0	13.0	41.0
64-65	33.21082777373741	36.5	34.0	39.5	9.0	41.0
66-67	32.870705420635936	36.0	34.0	39.0	6.5	41.0
68-69	32.61183736135729	35.5	34.0	39.0	2.0	41.0
70-71	32.23608745105165	35.0	34.0	38.5	2.0	40.5
72-73	31.844067849332617	35.0	34.0	37.0	2.0	39.5
74-75	31.457780804576362	35.0	33.0	37.0	2.0	39.0
76-77	31.067772411520682	35.0	33.0	36.0	2.0	38.5
78-79	30.731633980010088	35.0	33.0	36.0	2.0	37.0
80-81	30.40748616088853	35.0	33.0	35.5	2.0	37.0
82-83	30.12697836061088	35.0	32.5	35.0	2.0	36.5
84-85	29.83741716555123	35.0	32.0	35.0	2.0	36.0
86-87	29.560322466181812	35.0	32.0	35.0	2.0	36.0
88-89	29.301173395069142	35.0	31.5	35.0	2.0	35.5
90-91	29.03580912758528	35.0	31.0	35.0	2.0	35.0
92-93	28.718950655504266	35.0	31.0	35.0	2.0	35.0
94-95	28.368573235427956	35.0	30.5	35.0	2.0	35.0
96-97	28.03618704733202	35.0	30.0	35.0	2.0	35.0
98-99	27.69373651153753	35.0	29.0	35.0	2.0	35.0
100	27.130037610404294	34.0	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.40561744870522887
1101	2	0.5146419886544535
1101	3	0.5024358220535561
1101	4	0.4731940484889421
1101	5	0.4970631396511749
1101	6	0.45015156811491863
1101	7	0.42833039726802724
1101	8	0.3691841080818321
1101	9	0.4332446014701219
1101	10-11	0.43731215885831887
1101	12-13	0.5187412308958415
1101	14-15	0.6621888872520074
1101	16-17	0.6666003399843348
1101	18-19	0.6315547703637634
1101	20-21	0.6347879679491726
1101	22-23	0.6932661633086354
1101	24-25	0.6611237800607768
1101	26-27	0.7140898183631705
1101	28-29	0.7457365737888679
1101	30-31	0.7110742064519826
1101	32-33	0.6917831763220832
1101	34-35	0.7739854680670462
1101	36-37	0.8044621450991016
1101	38-39	0.9247131491655693
1101	40-41	0.9379613690767243
1101	42-43	0.8785365011130324
1101	44-45	0.8832261336410028
1101	46-47	0.9130706252708194
1101	48-49	0.8515549218430252
1101	50-51	0.834650416944946
1101	52-53	0.8959835622898709
1101	54-55	0.9907924341665435
1101	56-57	1.0687670692473432
1101	58-59	1.0046585828108903
1101	60-61	0.9878915763750129
1101	62-63	0.9857434346260661
1101	64-65	1.004352550080931
1101	66-67	0.9693582111787222
1101	68-69	0.985270422784339
1101	70-71	0.943434624176966
1101	72-73	0.9458139557594798
1101	74-75	0.9096273494163647
1101	76-77	0.8422808181147055
1101	78-79	0.7987323263070962
1101	80-81	0.8346544178126116
1101	82-83	0.7686727115861061
1101	84-85	0.762363051484872
1101	86-87	0.816157519153176
1101	88-89	0.7915279575087766
1101	90-91	0.8441229465825444
1101	92-93	0.8296672164115897
1101	94-95	0.7803053469760783
1101	96-97	0.768557460348017
1101	98-99	0.8013087110310124
1101	100	0.8838556123012467
1102	1	0.5264643986027195
1102	2	0.6185133307671578
1102	3	0.6107569964336079
1102	4	0.5225594460199119
1102	5	0.4852023651662165
1102	6	0.4361363149192883
1102	7	0.46508217707975774
1102	8	0.44628608211422005
1102	9	0.47271310044140336
1102	10-11	0.49875209806685206
1102	12-13	0.49605685566933744
1102	14-15	0.6630020020077652
1102	16-17	0.7071596433374481
1102	18-19	0.6654039964475373
1102	20-21	0.6807157328296896
1102	22-23	0.7182394085756769
1102	24-25	0.6648409750033935
1102	26-27	0.7315357328557255
1102	28-29	0.6903414275567812
1102	30-31	0.6569205434054126
1102	32-33	0.7105775677532904
1102	34-35	0.8567287778258716
1102	36-37	0.8803199764454135
1102	38-39	0.8723276133613496
1102	40-41	0.8677905098569312
1102	42-43	0.8697690247716494
1102	44-45	0.9093355206928564
1102	46-47	0.989469956109005
1102	48-49	1.0719559002496766
1102	50-51	1.0100728675925623
1102	52-53	1.0519855530440623
1102	54-55	1.244102261291765
1102	56-57	1.2201423001321174
1102	58-59	1.1868865537507602
1102	60-61	1.1249569160232866
1102	62-63	1.1027686979614728
1102	64-65	1.1571734476998259
1102	66-67	1.122927640178908
1102	68-69	1.0914293917390907
1102	70-71	1.0290839339988054
1102	72-73	1.0228358615147748
1102	74-75	1.0267473790970207
1102	76-77	1.0037456590609999
1102	78-79	0.9188277233788718
1102	80-81	0.9092995798522026
1102	82-83	0.8960229114404932
1102	84-85	0.8348023545219903
1102	86-87	0.8358489805795735
1102	88-89	0.8598747455544213
1102	90-91	0.8777508203892772
1102	92-93	0.9012762831745889
1102	94-95	0.8708767156491675
1102	96-97	0.877414736535389
1102	98-99	0.886457200703795
1102	100	0.9564723779878861
1103	1	0.4551736208470487
1103	2	0.49532691396884587
1103	3	0.5315270504239606
1103	4	0.3838696869245979
1103	5	0.436018863792377
1103	6	0.41357880142110304
1103	7	0.4324247024292944
1103	8	0.348841459761573
1103	9	0.4610600638127522
1103	10-11	0.5052748364847801
1103	12-13	0.4708335169868505
1103	14-15	0.6353062731260408
1103	16-17	0.6433582431765075
1103	18-19	0.5855200800590694
1103	20-21	0.6437069978665093
1103	22-23	0.6314709300431787
1103	24-25	0.6096040658105082
1103	26-27	0.6273320623353627
1103	28-29	0.6059443506246041
1103	30-31	0.632869556455411
1103	32-33	0.6904002659424435
1103	34-35	0.6208462130714381
1103	36-37	0.5637231148365274
1103	38-39	0.6382346421797394
1103	40-41	0.7287419034162497
1103	42-43	0.7160738867610448
1103	44-45	0.6932428669124846
1103	46-47	0.6738011895461966
1103	48-49	0.7304639054638145
1103	50-51	0.7223235198068778
1103	52-53	0.6597569516441837
1103	54-55	0.775108618483884
1103	56-57	0.8018038301608854
1103	58-59	0.8160699313071618
1103	60-61	0.7885126100003887
1103	62-63	0.8031621784732579
1103	64-65	0.8354536990682746
1103	66-67	0.8369490752396942
1103	68-69	0.8215318250732224
1103	70-71	0.7546382194334811
1103	72-73	0.6853612961764917
1103	74-75	0.7137426518900547
1103	76-77	0.6999836804678417
1103	78-79	0.7355396369857274
1103	80-81	0.6529490845883963
1103	82-83	0.6232274363011889
1103	84-85	0.7019484000489484
1103	86-87	0.6437142423230959
1103	88-89	0.6415405347840419
1103	90-91	0.6408614799635863
1103	92-93	0.648212947458962
1103	94-95	0.6547260968536577
1103	96-97	0.6602696196392266
1103	98-99	0.6548513081604241
1103	100	0.6823630986208613
1104	1	0.4094406437078746
1104	2	0.5008596690412617
1104	3	0.5084173355707762
1104	4	0.4112746523212323
1104	5	0.41899659315129156
1104	6	0.40029897515344004
1104	7	0.4106369165959052
1104	8	0.370235985974368
1104	9	0.4448836400094507
1104	10-11	0.5052393692053414
1104	12-13	0.4738573976513152
1104	14-15	0.591605666652427
1104	16-17	0.5588541055333351
1104	18-19	0.5783620973300003
1104	20-21	0.5549572413878892
1104	22-23	0.5992060058785142
1104	24-25	0.6513132913389015
1104	26-27	0.5727419471729362
1104	28-29	0.6439894014889589
1104	30-31	0.6666972330293532
1104	32-33	0.7116849616258847
1104	34-35	0.7127025182038054
1104	36-37	0.6586792096046423
1104	38-39	0.7334968600392173
1104	40-41	0.6901802162619362
1104	42-43	0.7790364157770426
1104	44-45	0.760948189016645
1104	46-47	0.6677655832080376
1104	48-49	0.7538235634625678
1104	50-51	0.6719572850166315
1104	52-53	0.7303578173830516
1104	54-55	0.7697863063500208
1104	56-57	0.7010087448128957
1104	58-59	0.7274020699571295
1104	60-61	0.746225832708042
1104	62-63	0.7566880560411136
1104	64-65	0.7879563934011387
1104	66-67	0.7994429471570683
1104	68-69	0.7397321517993731
1104	70-71	0.7022015189719113
1104	72-73	0.6496391834912707
1104	74-75	0.6409101860530306
1104	76-77	0.6138523759902945
1104	78-79	0.6251367972651174
1104	80-81	0.5834640751066615
1104	82-83	0.6039344728632905
1104	84-85	0.5847361658722754
1104	86-87	0.6077103586840984
1104	88-89	0.6025247427951967
1104	90-91	0.5031033908664071
1104	92-93	0.5385945051256655
1104	94-95	0.5062977385660119
1104	96-97	0.4277517423879367
1104	98-99	0.450876356378501
1104	100	0.49612230648306266
1105	1	0.3724575980311826
1105	2	0.41792772690020996
1105	3	0.4053031086682708
1105	4	0.4083788201439731
1105	5	0.39837127294141794
1105	6	0.38497460623621294
1105	7	0.36278439982509525
1105	8	0.40850517434357414
1105	9	0.4572440548933301
1105	10-11	0.46916190356179044
1105	12-13	0.45681438014668174
1105	14-15	0.5846575831925733
1105	16-17	0.5752012630175969
1105	18-19	0.5847821659519497
1105	20-21	0.5253973388090571
1105	22-23	0.5998485135990421
1105	24-25	0.5630662560230846
1105	26-27	0.5299273734305316
1105	28-29	0.5789459282672951
1105	30-31	0.5545846315722329
1105	32-33	0.6430733403907851
1105	34-35	0.6425791886944197
1105	36-37	0.5978316353703903
1105	38-39	0.564245597889041
1105	40-41	0.5778918916717259
1105	42-43	0.5900704092237206
1105	44-45	0.6997742498614841
1105	46-47	0.6369946923048175
1105	48-49	0.6207648234642988
1105	50-51	0.6843927005525217
1105	52-53	0.6782905329723263
1105	54-55	0.5981594385775892
1105	56-57	0.5347067055688655
1105	58-59	0.541380508300854
1105	60-61	0.5480567273609083
1105	62-63	0.5411980400817953
1105	64-65	0.5477843481821694
1105	66-67	0.44231906114412567
1105	68-69	0.4084202877901717
1105	70-71	0.43648817143829177
1105	72-73	0.40719061549262037
1105	74-75	0.4673243857938978
1105	76-77	0.4090127728586488
1105	78-79	0.42964182703557086
1105	80-81	0.4664649409895141
1105	82-83	0.4386753589966972
1105	84-85	0.29664376383926694
1105	86-87	0.3231266421493153
1105	88-89	0.31330009351515287
1105	90-91	0.34475659584473917
1105	92-93	0.3768217623019936
1105	94-95	0.30157483158122034
1105	96-97	0.2809931437521307
1105	98-99	0.32223669834970536
1105	100	0.3381951948707673
1106	1	0.23926704032840007
1106	2	0.3153311726209367
1106	3	0.3159779752518048
1106	4	0.32419464216240357
1106	5	0.24212567820217146
1106	6	0.2600152056356748
1106	7	0.2706306049107994
1106	8	0.32418932263400535
1106	9	0.3420615576881687
1106	10-11	0.33698716994290123
1106	12-13	0.3877626117168802
1106	14-15	0.479710136501069
1106	16-17	0.44829786764269386
1106	18-19	0.4689344582366459
1106	20-21	0.479512271900731
1106	22-23	0.4393418537484237
1106	24-25	0.48399928056861796
1106	26-27	0.41805943959786873
1106	28-29	0.4146980563471132
1106	30-31	0.4684352877463098
1106	32-33	0.43656117089828683
1106	34-35	0.3979096510241149
1106	36-37	0.4061993716924661
1106	38-39	0.3764301941283392
1106	40-41	0.34527308195058737
1106	42-43	0.4352962038488428
1106	44-45	0.47307778878656137
1106	46-47	0.4976219239808728
1106	48-49	0.4463947273424722
1106	50-51	0.3967345307140704
1106	52-53	0.43594942572536866
1106	54-55	0.419392343584029
1106	56-57	0.43305420028255526
1106	58-59	0.43125776773166535
1106	60-61	0.44217801177357074
1106	62-63	0.45833408486119254
1106	64-65	0.44439026420531036
1106	66-67	0.4709711352635182
1106	68-69	0.49490591599003864
1106	70-71	0.45978448121751114
1106	72-73	0.4606347871228671
1106	74-75	0.5120280037232128
1106	76-77	0.5782734119667801
1106	78-79	0.5092489229585944
1106	80-81	0.5719902029814996
1106	82-83	0.49484194949914695
1106	84-85	0.4858157942739716
1106	86-87	0.5119288243186269
1106	88-89	0.4963216699336783
1106	90-91	0.5127809219205268
1106	92-93	0.5098867135956233
1106	94-95	0.42604110116123195
1106	96-97	0.5112077461103794
1106	98-99	0.5266002624218835
1106	100	0.6141311756205852
1107	1	0.21141539583697977
1107	2	0.2505729514697279
1107	3	0.2988910046871496
1107	4	0.2738973809355585
1107	5	0.36729411462291495
1107	6	0.30157928112235766
1107	7	0.316814417602707
1107	8	0.2796320455451067
1107	9	0.3663499415604079
1107	10-11	0.3336011060054034
1107	12-13	0.3672436103300356
1107	14-15	0.37004264988848945
1107	16-17	0.43323445205940203
1107	18-19	0.4063433732875765
1107	20-21	0.43138682627006375
1107	22-23	0.3899416976525458
1107	24-25	0.4080545870885075
1107	26-27	0.37342446280330677
1107	28-29	0.4040940162235884
1107	30-31	0.3852770471810629
1107	32-33	0.31030845358505843
1107	34-35	0.3726085592235435
1107	36-37	0.4057249899601203
1107	38-39	0.34421500381412073
1107	40-41	0.3950158676308604
1107	42-43	0.3869721233824137
1107	44-45	0.3494759155489504
1107	46-47	0.3748324479598253
1107	48-49	0.43107621949023667
1107	50-51	0.4161682935649935
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2316	96-97	-0.7685059208600613
2316	98-99	-0.5911148905100561
2316	100	-0.6377711342869787
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	20515.0
3	3370.0
4	3328.0
5	3837.0
6	4671.0
7	5554.0
8	6823.0
9	8573.0
10	11844.0
11	18459.0
12	30821.0
13	45754.0
14	46557.0
15	53978.0
16	70226.0
17	96272.0
18	96985.0
19	55200.0
20	45941.0
21	40810.0
22	41472.0
23	46408.0
24	51422.0
25	52407.0
26	55444.0
27	64065.0
28	75133.0
29	89671.0
30	107315.0
31	131223.0
32	165646.0
33	217856.0
34	313018.0
35	496413.0
36	908785.0
37	1756257.0
38	1543669.0
39	318445.0
40	516.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.988074987409355	17.412870983760712	13.576764186926157	23.022289841903778
2	14.076853259481029	22.41170400922589	37.09495921325841	26.416483518034674
3	19.156115749120087	28.61987025244	29.470098181620045	22.753915816819866
4	13.066180472176164	18.58467146226099	34.4065469673901	33.942601098172744
5	12.884653972933172	36.83663300834819	33.110513542240184	17.168199476478456
6	29.799703630177838	33.656219465942144	18.714414824694853	17.829662079185166
7	24.17521671312979	33.1207297067514	22.074695326814442	20.62935825330437
8	27.735562010094565	30.60892203747655	21.672576122303177	19.982939830125705
9	28.243252411019327	14.701727815498083	19.6016220475706	37.453397725911984
10-11	24.3439224960528	26.52214100224474	25.58819355333828	23.545742948364182
12-13	27.305269923105875	24.33115797635009	26.198846746858923	22.164725353685107
14-15	26.076412091523288	22.743805106636106	25.908158208984055	25.27162459285655
16-17	22.40826205582741	26.36283580514605	28.158688494360945	23.070213644665593
18-19	24.060321646072573	25.075355174313437	30.116795056345243	20.747528123268754
20-21	23.856828309871045	24.5916182325224	29.495217870959774	22.05633558664678
22-23	22.41954996950622	26.317015020450324	26.62146636473914	24.641968645304313
24-25	22.30218911755881	27.20895179809164	26.020589812559297	24.46826927179025
26-27	23.476652466474278	24.954902507478405	26.512594085617753	25.055850940429565
28-29	22.69248709544017	24.194671600544897	29.860785719308815	23.252055584706117
30-31	24.816455271505646	24.320527649773	28.640661577396287	22.222355501325072
32-33	20.803008470882066	25.893571829823948	29.197196085274808	24.10622361401918
34-35	20.43963944406128	26.61710438071166	29.500318413311405	23.44293776191565
36-37	22.773604076369338	26.58270412999094	27.478554178960824	23.165137614678898
38-39	23.91330462952636	24.654042656358868	27.252343354723912	24.18030935939086
40-41	22.413669814114208	26.240183262902246	27.171954734074415	24.17419218890913
42-43	24.784158587183203	25.30891838700588	26.849636665457773	23.05728636035315
44-45	24.468637340897757	24.69887100234166	27.810563151394945	23.021928505365636
46-47	22.836353907235676	25.251477859730066	29.36427437928199	22.547893853752267
48-49	23.450786905695153	26.24941430949452	28.325241095741248	21.974557689069076
50-51	22.14855489213661	26.913125250063352	26.696077490317133	24.24224236748291
52-53	21.625861958999486	27.58527443322749	24.80089447461773	25.987969133155286
54-55	22.60978374831788	26.711842511665225	24.691657424765527	25.986716315251375
56-57	23.458591040169676	24.80416864112235	25.313121953539664	26.424118365168315
58-59	23.222053460093107	24.59477766448922	27.730368000031575	24.452800875386103
60-61	23.377672963840716	25.35027861850878	26.63242050556127	24.639627912089228
62-63	20.56790485581225	25.925780536706572	27.64392011610485	25.862394491376318
64-65	20.731341021565843	25.934492831923432	27.37326566831954	25.960900478191178
66-67	23.022021010883112	25.63319707130589	25.925941061975738	25.418840855835263
68-69	23.67927860752247	24.441505711507542	26.007053804456792	25.872161876513193
70-71	22.349731108064503	25.400339448982056	26.27788236545978	25.97204707749366
72-73	24.049493175805065	24.823460185993426	26.176859257605184	24.950187380596326
74-75	24.45424016986403	24.080349769167242	26.748591987869435	24.716818073099297
76-77	23.061607358950315	25.35639725072179	27.68942600325721	23.892569387070683
78-79	23.16174098400261	25.34556219618857	27.626168677720003	23.866528142088814
80-81	22.31992133347592	25.530923656753462	27.006533892250022	25.14262111752059
82-83	22.491387231832842	26.30903433957642	25.24294835552898	25.95663007306176
84-85	22.938969728442736	26.153966438194015	25.121017279763073	25.786046553600173
86-87	23.255227244346983	24.534265306866402	25.877763649915664	26.33274379887095
88-89	23.714402073551526	23.783312258448653	27.054321881130317	25.447963786869504
90-91	24.266968062936776	24.677813647543456	26.203801461974948	24.851416827544824
92-93	23.014307220691407	24.961919871249457	26.66225566410427	25.361517243954868
94-95	22.77121490188555	25.134655464673134	26.92364091819579	25.170488715245526
96-97	24.433000438927298	24.822292364572142	25.473111217566245	25.271595978934315
98-99	24.996842860380507	24.042026316163277	25.28922190341845	25.67190892003776
100	24.770567876406325	24.488090907278586	25.68429512035493	25.057046095960168
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2162.0
1	3120.0
2	2546.0
3	879.0
4	663.5
5	697.5
6	916.5
7	1344.0
8	1964.0
9	2113.0
10	1774.0
11	1682.5
12	1844.5
13	2103.5
14	2460.0
15	2936.0
16	3568.0
17	4270.5
18	5011.5
19	5995.0
20	7475.0
21	9222.0
22	11654.5
23	14598.0
24	17992.5
25	22441.0
26	28375.0
27	35447.0
28	42268.0
29	50223.0
30	58820.5
31	67109.5
32	75672.5
33	84049.5
34	91683.5
35	96377.0
36	100314.5
37	107191.5
38	111980.5
39	115218.5
40	117201.5
41	119196.0
42	124435.0
43	137253.5
44	156355.5
45	176322.5
46	206454.5
47	252806.0
48	393537.5
49	554596.0
50	692841.0
51	631036.0
52	401125.5
53	299364.5
54	249187.0
55	207474.5
56	181903.0
57	159867.0
58	147770.5
59	135385.0
60	114142.0
61	93759.0
62	76227.0
63	59630.5
64	43984.5
65	34945.5
66	26950.5
67	19559.0
68	15657.5
69	13315.0
70	11664.0
71	10201.0
72	8668.0
73	8932.5
74	7504.5
75	5733.0
76	4365.5
77	2897.0
78	2107.0
79	1449.0
80	977.0
81	709.5
82	559.0
83	418.5
84	302.0
85	199.5
86	131.5
87	91.5
88	73.0
89	53.0
90	47.5
91	46.5
92	39.0
93	33.0
94	33.0
95	27.0
96	19.0
97	12.5
98	8.5
99	7.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0866752253408069
2	0.2853470028149039
3	0.28804944569659197
4	0.2969590620721572
5	0.32409609267577455
6	0.1716191982105324
7	0.188551691891109
8	0.15436297439308694
9	0.20594866794197575
10-11	0.2268222241583474
12-13	0.2440714103641218
14-15	0.1706972710816232
16-17	0.15960599508802856
18-19	0.1773759645574616
20-21	0.20974194063267848
22-23	0.17245667399938885
24-25	0.1976231733351087
26-27	0.19612416204917235
28-29	0.2252950624257268
30-31	0.2599341870706969
32-33	0.22752598532545365
34-35	0.15363810039096748
36-37	0.14816987612255184
38-39	0.11512828932691296
40-41	0.10635942518476899
42-43	0.12209552488126495
44-45	0.13676894521543045
46-47	0.2033377140120115
48-49	0.20776437175310988
50-51	0.21435861388889554
52-53	0.1409703993830548
54-55	0.08621778058218783
56-57	0.12450238807276834
58-59	0.1437572936047956
60-61	0.1197871882531564
62-63	0.14161785965679255
64-65	0.11616985585423023
66-67	0.12035723479851249
68-69	0.1058456795327814
70-71	0.1317511280939628
72-73	0.15622090387424745
74-75	0.19473071493830196
76-77	0.174835386744208
78-79	0.20049451889690226
80-81	0.10390329871156814
82-83	0.1446088446169942
84-85	0.1761373449033546
86-87	0.18993810139030834
88-89	0.17055651884820194
90-91	0.1739556852853252
92-93	0.12372825078895147
94-95	0.3668284707424666
96-97	0.3668284707424666
98-99	0.41450828981391574
100	0.44891517327374075
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7104683.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.20664952049909
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.94156243836247	27.345330084501597
2	11.574854634036896	7.918739914344497
3	3.2319128255040805	3.31658727908472
4	1.4252489003786932	1.9501195845892272
5	0.7767721426112186	1.3285386219794546
6	0.5169161664893371	1.0609182083148427
7	0.34019793088381317	0.8145921972539099
8	0.2617219737362966	0.7162105461928613
9	0.20384422598618882	0.6275545195578277
>10	1.3732546006061779	9.226138797352574
>50	0.1638737653536669	3.9422043131049715
>100	0.15491081603691223	11.021732408321347
>500	0.020451645725511995	4.744998002867332
>1k	0.012113343301694711	7.9169127347298165
>5k	8.71165100464019E-4	2.2420680971609364
>10k+	0.001493425886509747	15.827354690644372
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	131334	1.8485553824146694	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	107469	1.5126501773548517	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	71913	1.0121915362022484	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	53550	0.7537282099708038	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	53137	0.7479151427305061	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	53120	0.7476758639336899	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	52773	0.7427917614339725	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	51811	0.7292513965788481	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	43612	0.6138486403967637	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	42096	0.5925106018101018	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	35793	0.5037944690846868	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	33631	0.4733638362190122	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27859	0.3921216470882656	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	23999	0.33779128498766237	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	23109	0.32526433621317097	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	22187	0.3122869802917315	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC	20722	0.2916667780955181	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19656	0.2766625900128127	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC	18210	0.25630981706009964	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	17575	0.24737205023785017	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT	16598	0.23362055703259385	No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	16318	0.2296794944967988	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	14815	0.20852443381358465	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	14610	0.20563901302844897	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG	14394	0.20259876478654995	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	14248	0.20054378217859967	No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	13678	0.1925209048735883	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	13336	0.1877071784905815	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13213	0.18597592601950008	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA	12999	0.1829638282242853	No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG	12900	0.18157038111341492	No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA	11256	0.1584307139389611	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	11141	0.1568120632546167	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC	11100	0.15623497909758957	No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	10781	0.15174498285145166	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	10421	0.14667790244828657	No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	9747	0.1371912019156942	No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	9440	0.13287010834966176	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG	9207	0.1295905813109466	No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC	9163	0.1289712714838931	No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC	8954	0.1260295498053889	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	8805	0.12393234152741227	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT	8486	0.11944234528127434	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	8347	0.11748588923671895	No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC	8007	0.11270031330039637	No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA	7979	0.11230620704681686	No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT	7798	0.10975859162189221	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	7342	0.10334028977788312	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.8150446684250375E-4	0.0	0.0	1.4075223342125188E-5	0.0
2	4.222567002637556E-4	0.0	0.0	2.8150446684250376E-5	0.0
3	4.363319236058808E-4	0.0	0.0	2.8150446684250376E-5	0.0
4	6.474602737377586E-4	0.0	0.0	2.8150446684250376E-5	0.0
5	0.0024631640848719076	0.0	0.0	5.630089336850075E-5	0.0
6	0.005278208753296945	0.0	0.0	5.630089336850075E-5	0.0
7	0.006347925727298459	0.0	0.0	5.630089336850075E-5	0.0
8	0.00716428868114172	0.0	0.0	7.037611671062594E-5	0.0
9	0.00792435074161648	0.0	0.0	8.445134005275112E-5	0.0
10-11	0.013702229923558868	0.0	0.0	1.7594029177656485E-4	0.0
12-13	0.026503645553221725	0.0	0.0	2.6742924350037857E-4	0.0
14-15	0.04021291308845166	0.0	0.0	3.4484297188206706E-4	0.0
16-17	0.04514627886986654	0.0	0.0	5.348584870007571E-4	0.0
18-19	0.04760240534306738	0.0	0.0	7.811748954879478E-4	0.0
20-21	0.05704688020563338	0.0	0.0	0.0016186506843443966	0.0
22-23	0.06061494932286212	0.0	1.4075223342125188E-5	0.0044266577410983715	0.0
24-25	0.08312826905859136	0.0	1.4075223342125188E-5	0.011189802556989523	0.0
26-27	0.15346216009919092	0.0	1.4075223342125188E-5	0.02341413402962525	0.0
28-29	0.16856487474529125	0.0	1.4075223342125188E-5	0.07139657040293002	0.0
30-31	0.1716191982105324	0.0	1.4075223342125188E-5	0.18789015639402912	0.0
32-33	0.17356157903174568	0.0	1.4075223342125188E-5	0.3629014834300137	1.4075223342125188E-5
34-35	0.17542654612457725	0.0	1.4075223342125188E-5	0.5799414273655841	1.4075223342125188E-5
36-37	0.17873422360997668	1.4075223342125188E-5	1.4075223342125188E-5	0.8966832158450982	1.4075223342125188E-5
38-39	0.1821193148237578	2.8150446684250376E-5	1.4075223342125188E-5	1.3656415071580252	1.4075223342125188E-5
40-41	0.1854692179791836	2.8150446684250376E-5	1.4075223342125188E-5	2.0714858073189193	1.4075223342125188E-5
42-43	0.19312613947729967	2.8150446684250376E-5	1.4075223342125188E-5	2.569460171551637	1.4075223342125188E-5
44-45	0.20197241734782537	2.8150446684250376E-5	1.4075223342125188E-5	3.051923639661333	1.4075223342125188E-5
46-47	0.205751614815186	2.8150446684250376E-5	1.4075223342125188E-5	3.5961914134663013	1.4075223342125188E-5
48-49	0.20852443381358465	2.8150446684250376E-5	1.4075223342125188E-5	4.079443094083156	1.4075223342125188E-5
50-51	0.21200101397908955	2.8150446684250376E-5	1.4075223342125188E-5	4.62825716502763	1.4075223342125188E-5
52-53	0.2134648372066706	2.8150446684250376E-5	1.4075223342125188E-5	5.301862166123387	2.1112835013187783E-5
54-55	0.21806039762787446	4.222567002637556E-5	1.4075223342125188E-5	5.860972262942625	2.8150446684250376E-5
56-57	0.2286801536395079	4.222567002637556E-5	1.4075223342125188E-5	6.422313282661591	2.8150446684250376E-5
58-59	0.2320159815715916	4.222567002637556E-5	1.4075223342125188E-5	6.999052596716842	2.8150446684250376E-5
60-61	0.23403577612118653	4.222567002637556E-5	1.4075223342125188E-5	7.497505518543192	2.8150446684250376E-5
62-63	0.2355347874071229	4.222567002637556E-5	1.4075223342125188E-5	8.022342446524355	2.8150446684250376E-5
64-65	0.23668895572117715	4.222567002637556E-5	1.4075223342125188E-5	8.594246921361588	2.8150446684250376E-5
66-67	0.23869467504742997	5.630089336850075E-5	1.4075223342125188E-5	9.154799728573392	2.8150446684250376E-5
68-69	0.24036962662514288	5.630089336850075E-5	1.4075223342125188E-5	9.82841880489249	2.8150446684250376E-5
70-71	0.24304391906014666	5.630089336850075E-5	1.4075223342125188E-5	10.664423732909688	2.8150446684250376E-5
72-73	0.24925813016569495	5.630089336850075E-5	1.4075223342125188E-5	11.323847946488254	2.8150446684250376E-5
74-75	0.25512046068769006	7.037611671062594E-5	1.4075223342125188E-5	11.956550348551794	2.8150446684250376E-5
76-77	0.25699950300386376	7.037611671062594E-5	1.4075223342125188E-5	12.622378788751025	2.8150446684250376E-5
78-79	0.25847036384311584	7.037611671062594E-5	1.4075223342125188E-5	13.240801876733979	2.8150446684250376E-5
80-81	0.2610953929964222	7.741372838168853E-5	1.4075223342125188E-5	13.94907415292139	3.518805835531297E-5
82-83	0.26234105026220034	9.148895172381372E-5	1.4075223342125188E-5	14.800751842130044	4.222567002637556E-5
84-85	0.2668380841200093	9.852656339487631E-5	1.4075223342125188E-5	15.56370636100161	4.222567002637556E-5
86-87	0.27528321812528445	9.852656339487631E-5	1.4075223342125188E-5	16.301416966809075	4.222567002637556E-5
88	0.27726782461652405	9.852656339487631E-5	1.4075223342125188E-5	16.885510585060587	4.222567002637556E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCTAC	11570	0.0	27.671743	1
ATCTACA	12415	0.0	25.878685	2
AGTACTC	3225	0.0	23.77598	5
TCTACAC	14745	0.0	23.445621	3
TACACTC	15545	0.0	22.393476	5
CTACACT	17060	0.0	21.674168	4
TATCACG	480	0.0	21.560045	2
GATGTAA	2300	0.0	20.266014	1
CACTCTT	17705	0.0	19.622581	7
ACACTCT	18355	0.0	18.876469	6
GTACTAG	1655	0.0	18.776165	1
AGTACTT	39180	0.0	18.5338	12-13
ACTCTTT	20430	0.0	18.362938	8
AAAGTAC	4145	0.0	18.155767	3
AAGTACT	4345	0.0	17.756323	4
ATTGCGT	1100	0.0	17.522612	6
GAGTACT	38520	0.0	17.34447	12-13
GTATAAG	1905	0.0	17.300714	1
TCACGCA	655	0.0	17.237286	4
CATGGGT	7520	0.0	17.078264	4
>>END_MODULE