##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765556_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7104683
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.62364598110852	34.0	31.0	34.0	31.0	34.0
2	32.73820450539454	34.0	31.0	34.0	31.0	34.0
3	32.786940106968885	34.0	31.0	34.0	31.0	34.0
4	36.25466203066344	37.0	37.0	37.0	35.0	37.0
5	36.192752864554265	37.0	37.0	37.0	35.0	37.0
6	36.275373862563605	37.0	37.0	37.0	35.0	37.0
7	36.21637024480895	37.0	37.0	37.0	35.0	37.0
8	36.23089517153686	37.0	37.0	37.0	35.0	37.0
9	37.98621669678999	39.0	38.0	39.0	35.0	39.0
10-11	37.936256832289345	39.0	38.0	39.0	35.0	39.0
12-13	37.92471697892784	39.0	38.0	39.0	35.0	39.0
14-15	39.268225690013196	41.0	39.0	41.0	36.0	41.0
16-17	39.244940977662196	40.5	39.0	41.0	36.0	41.0
18-19	39.28682799781497	41.0	39.0	41.0	36.0	41.0
20-21	39.29463545382672	41.0	39.0	41.0	36.0	41.0
22-23	39.24031058106322	41.0	39.0	41.0	36.0	41.0
24-25	39.19039132358192	41.0	39.0	41.0	36.0	41.0
26-27	39.02911326233696	40.5	39.0	41.0	35.0	41.0
28-29	38.877936144371255	40.0	38.0	41.0	35.0	41.0
30-31	38.64574921921218	40.0	38.0	41.0	35.0	41.0
32-33	38.30980031058388	40.0	38.0	41.0	34.0	41.0
34-35	38.2393477654105	40.0	38.0	41.0	34.0	41.0
36-37	38.1149218761766	40.0	38.0	41.0	34.0	41.0
38-39	37.88631639159692	40.0	37.5	41.0	33.5	41.0
40-41	37.60875017224555	40.0	37.0	41.0	33.0	41.0
42-43	37.29364300982887	40.0	37.0	41.0	33.0	41.0
44-45	36.859122919347705	40.0	36.0	41.0	32.0	41.0
46-47	36.44009781998719	40.0	36.0	41.0	31.0	41.0
48-49	36.090440699465404	39.5	35.5	41.0	30.5	41.0
50-51	35.782707546557674	39.0	35.0	41.0	29.5	41.0
52-53	35.616119185050195	39.0	35.0	41.0	28.5	41.0
54-55	35.557087768729446	39.0	35.0	41.0	28.5	41.0
56-57	35.343372533299515	39.0	35.0	41.0	28.0	41.0
58-59	35.20374329157262	39.0	35.0	41.0	28.0	41.0
60-61	34.84927300767677	38.0	35.0	40.5	28.0	41.0
62-63	34.463889733011314	37.0	35.0	40.0	26.0	41.0
64-65	34.2439310803874	37.0	35.0	40.0	26.0	41.0
66-67	33.83432068679208	36.5	35.0	39.0	26.0	41.0
68-69	33.47975905469674	36.0	34.5	39.0	25.5	41.0
70-71	33.07912147522979	35.0	34.0	38.5	24.5	40.5
72-73	32.65821705204863	35.0	34.0	37.0	23.5	39.0
74-75	32.29720052252859	35.0	34.0	37.0	22.0	39.0
76-77	30.901351545170982	34.5	31.5	35.5	20.0	37.0
78-79	31.612047434065673	35.0	33.0	36.0	20.0	37.0
80-81	31.53760315555247	35.0	34.0	35.5	20.0	37.0
82-83	31.375903470992302	35.0	34.0	35.0	18.5	36.5
84-85	31.1805852562317	35.0	34.0	35.0	16.0	36.0
86-87	31.00183759078343	35.0	33.0	35.0	9.5	36.0
88-89	30.84918264474291	35.0	33.0	35.0	4.5	35.5
90-91	30.71423700395922	35.0	33.0	35.0	2.0	35.0
92-93	30.564676355018232	35.0	33.0	35.0	2.0	35.0
94-95	30.48217436302225	35.0	33.0	35.0	2.0	35.0
96-97	30.374598979292955	35.0	33.0	35.0	2.0	35.0
98-99	30.243685186235613	35.0	33.0	35.0	2.0	35.0
100	29.936862066893063	35.0	33.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.40842020597823847
1101	2	0.3637860704901996
1101	3	0.34760649062457816
1101	4	0.21212836609375074
1101	5	0.26412915654331925
1101	6	0.2704151891859894
1101	7	0.29853563725863097
1101	8	0.2837778318460096
1101	9	0.34158338303804925
1101	10-11	0.35467515163810504
1101	12-13	0.34727266827298564
1101	14-15	0.524754459122363
1101	16-17	0.5062696874944592
1101	18-19	0.4665916281438598
1101	20-21	0.43921989313672327
1101	22-23	0.4529193192101175
1101	24-25	0.4792438541867341
1101	26-27	0.4980424534867538
1101	28-29	0.5670801309035554
1101	30-31	0.556099130979284
1101	32-33	0.47704642340728043
1101	34-35	0.5701579466702427
1101	36-37	0.5714454827411686
1101	38-39	0.5724008739158037
1101	40-41	0.6019172607698806
1101	42-43	0.6863373667975452
1101	44-45	0.7182385467028354
1101	46-47	0.6306272127473491
1101	48-49	0.6091061291628321
1101	50-51	0.6689633390725547
1101	52-53	0.731367998152642
1101	54-55	0.7937085298762767
1101	56-57	0.7597760945580276
1101	58-59	0.751503862672692
1101	60-61	0.7764396462468355
1101	62-63	0.7964518491172043
1101	64-65	0.7602652394374232
1101	66-67	0.7057835802343888
1101	68-69	0.6917396136527003
1101	70-71	0.6633386244724946
1101	72-73	0.7124194673893953
1101	74-75	0.7231387080912555
1101	76-77	0.6990229593929733
1101	78-79	0.7034356798450965
1101	80-81	0.6773971813612611
1101	82-83	0.6546952328992752
1101	84-85	0.6307851818262087
1101	86-87	0.7101819306015855
1101	88-89	0.6966907029299882
1101	90-91	0.7154219705964486
1101	92-93	0.7549589350679256
1101	94-95	0.8383097052207731
1101	96-97	0.8442152516556121
1101	98-99	0.880503466986319
1101	100	0.8521550676588241
1102	1	0.3387182843742096
1102	2	0.30580615724153404
1102	3	0.3108056123410421
1102	4	0.20844875036608812
1102	5	0.21052693857650695
1102	6	0.21848093443170313
1102	7	0.21952540076230775
1102	8	0.22276372746020456
1102	9	0.31051846519600446
1102	10-11	0.2933516481965057
1102	12-13	0.3018664487618352
1102	14-15	0.44130556340837046
1102	16-17	0.426172045741275
1102	18-19	0.39553841273372825
1102	20-21	0.3886488920727089
1102	22-23	0.41397216103123924
1102	24-25	0.4193122137372356
1102	26-27	0.4076229753424556
1102	28-29	0.4019952061266707
1102	30-31	0.46620406228186795
1102	32-33	0.4898792217403738
1102	34-35	0.45663205101891435
1102	36-37	0.5110417258645938
1102	38-39	0.5451695080998675
1102	40-41	0.5145923869143942
1102	42-43	0.478713667682797
1102	44-45	0.5484003862815712
1102	46-47	0.5340110510978064
1102	48-49	0.5031630990638618
1102	50-51	0.49606022228583413
1102	52-53	0.5856662058412496
1102	54-55	0.5996919843836963
1102	56-57	0.7267519116407826
1102	58-59	0.7084626158333691
1102	60-61	0.6863751406116378
1102	62-63	0.7403906160210383
1102	64-65	0.764110452350586
1102	66-67	0.7523871100431379
1102	68-69	0.7477699713637094
1102	70-71	0.6980045847506346
1102	72-73	0.7337938890935902
1102	74-75	0.6974146653595952
1102	76-77	0.5930314602897901
1102	78-79	0.6937927399834471
1102	80-81	0.7095546975677642
1102	82-83	0.6802436227007327
1102	84-85	0.727106280544259
1102	86-87	0.7781713698979829
1102	88-89	0.8149527127073171
1102	90-91	0.8205546555970535
1102	92-93	0.8622986435860369
1102	94-95	0.8783168710797895
1102	96-97	0.9079817974865314
1102	98-99	0.8589829615918312
1102	100	0.9653069021964704
1103	1	0.2733941678205696
1103	2	0.24486717221356002
1103	3	0.2603154500532838
1103	4	0.20828725194648712
1103	5	0.2380339649185501
1103	6	0.22133025348936286
1103	7	0.23474134373389433
1103	8	0.24376765949728707
1103	9	0.2409428268486451
1103	10-11	0.274263657095176
1103	12-13	0.30818649565055267
1103	14-15	0.42780791480869595
1103	16-17	0.43123504798320056
1103	18-19	0.4144195007270923
1103	20-21	0.38465707438150787
1103	22-23	0.3809148840362653
1103	24-25	0.38352142865966954
1103	26-27	0.4473442051401193
1103	28-29	0.39075506791783
1103	30-31	0.45229672975359136
1103	32-33	0.474894796545577
1103	34-35	0.4140332265464437
1103	36-37	0.37703388810162863
1103	38-39	0.41195484604349275
1103	40-41	0.36295913666970137
1103	42-43	0.3530810825996795
1103	44-45	0.4371144475257296
1103	46-47	0.46939995631710474
1103	48-49	0.41465654810935604
1103	50-51	0.4737023048808737
1103	52-53	0.5131338457368031
1103	54-55	0.5389437109524025
1103	56-57	0.541228503775308
1103	58-59	0.5487018966064312
1103	60-61	0.5570919501267255
1103	62-63	0.6039009678137717
1103	64-65	0.5577061411464044
1103	66-67	0.5870533410867083
1103	68-69	0.5358668116021832
1103	70-71	0.5195511526681358
1103	72-73	0.5512146426584295
1103	74-75	0.5615377348939177
1103	76-77	0.5116156900197169
1103	78-79	0.5602897856874414
1103	80-81	0.5802289746019049
1103	82-83	0.5969732547022026
1103	84-85	0.576206544453985
1103	86-87	0.5601886399306011
1103	88-89	0.5478690610500614
1103	90-91	0.551780613137435
1103	92-93	0.5430092288936947
1103	94-95	0.5537911543762348
1103	96-97	0.6017935179500888
1103	98-99	0.564281815220923
1103	100	0.5993274937509554
1104	1	0.28595582383562146
1104	2	0.2652496443852357
1104	3	0.25625793145657383
1104	4	0.18609400934568754
1104	5	0.1936262049700943
1104	6	0.1981840315078145
1104	7	0.2044694024438769
1104	8	0.20389335980743084
1104	9	0.17950619181324612
1104	10-11	0.2534738592384329
1104	12-13	0.2685838213614673
1104	14-15	0.4197716813011141
1104	16-17	0.3637387917203583
1104	18-19	0.3560359183340225
1104	20-21	0.34644983518938943
1104	22-23	0.3115331708980946
1104	24-25	0.3179010457996583
1104	26-27	0.403294125116723
1104	28-29	0.4030838680344786
1104	30-31	0.39206200549960357
1104	32-33	0.4402351846261823
1104	34-35	0.3801308635263041
1104	36-37	0.3639861870277272
1104	38-39	0.40288878269904416
1104	40-41	0.41612569865824867
1104	42-43	0.38678902197547416
1104	44-45	0.42982056778420485
1104	46-47	0.4195930478306664
1104	48-49	0.3899693543378291
1104	50-51	0.38742311702424104
1104	52-53	0.4048798257089814
1104	54-55	0.4507130217619064
1104	56-57	0.43092481505458835
1104	58-59	0.4431108875111818
1104	60-61	0.42513049884387755
1104	62-63	0.4300736110636407
1104	64-65	0.3995802923345977
1104	66-67	0.36527749665351195
1104	68-69	0.40799667400600725
1104	70-71	0.41230703672351154
1104	72-73	0.4419912304993048
1104	74-75	0.4128787128218079
1104	76-77	0.40769040648102006
1104	78-79	0.4363079383019688
1104	80-81	0.43082917258913866
1104	82-83	0.4461475949855114
1104	84-85	0.4241812222778769
1104	86-87	0.44089395371597817
1104	88-89	0.4166626946565408
1104	90-91	0.42329307226996704
1104	92-93	0.44695570005677254
1104	94-95	0.4655845081790382
1104	96-97	0.5403367182768157
1104	98-99	0.5216105608157591
1104	100	0.5668872988349278
1105	1	0.23301289560419036
1105	2	0.22379312685547603
1105	3	0.2313188248125897
1105	4	0.17302486071722
1105	5	0.17659074517420947
1105	6	0.14450180564524828
1105	7	0.1771081910543586
1105	8	0.16941691149808946
1105	9	0.2223478195910218
1105	10-11	0.22291203505451307
1105	12-13	0.22147631533720613
1105	14-15	0.3369995142467559
1105	16-17	0.31491874732407865
1105	18-19	0.3031069910782662
1105	20-21	0.3082624622197372
1105	22-23	0.2938922745835626
1105	24-25	0.2581454323819443
1105	26-27	0.2928881567710633
1105	28-29	0.34079132393703304
1105	30-31	0.3109178201470755
1105	32-33	0.3768109697171269
1105	34-35	0.4012066359869024
1105	36-37	0.37871958155032104
1105	38-39	0.4491674770774452
1105	40-41	0.42869475754212516
1105	42-43	0.4543567827268049
1105	44-45	0.40009179524884075
1105	46-47	0.4083098754457808
1105	48-49	0.40774863358041813
1105	50-51	0.40891949519087234
1105	52-53	0.35575336105704736
1105	54-55	0.3893248407783929
1105	56-57	0.38057365572512225
1105	58-59	0.3860930348709246
1105	60-61	0.35851503501345405
1105	62-63	0.3614365241929818
1105	64-65	0.389497027904504
1105	66-67	0.31856761577683557
1105	68-69	0.3943151332049055
1105	70-71	0.4049336335969187
1105	72-73	0.38609904093269876
1105	74-75	0.4472097072620471
1105	76-77	0.3415230891596792
1105	78-79	0.39028890886241285
1105	80-81	0.42422164717102717
1105	82-83	0.425785987572052
1105	84-85	0.4146143286903623
1105	86-87	0.40197672384629257
1105	88-89	0.41228953537493496
1105	90-91	0.45152613581615597
1105	92-93	0.4046082055243261
1105	94-95	0.43678120910339047
1105	96-97	0.36610693718444054
1105	98-99	0.43335361081084756
1105	100	0.38921120255596975
1106	1	0.15986555786624024
1106	2	0.1510188646801751
1106	3	0.1464460706887465
1106	4	0.1014170728616719
1106	5	0.12090334376814127
1106	6	0.11781902831069146
1106	7	0.12253278719038008
1106	8	0.0861193366183386
1106	9	0.1422734110255135
1106	10-11	0.17311470932210682
1106	12-13	0.1733196184372261
1106	14-15	0.20465851027330473
1106	16-17	0.22830105812054313
1106	18-19	0.15563151208281312
1106	20-21	0.17205618887024343
1106	22-23	0.12126904991978193
1106	24-25	0.1747416148765666
1106	26-27	0.2428561419822941
1106	28-29	0.20295668548469337
1106	30-31	0.21708415246575186
1106	32-33	0.20122921711399044
1106	34-35	0.10278968983629255
1106	36-37	0.17687472934762383
1106	38-39	0.19454456739787673
1106	40-41	0.1854221232155595
1106	42-43	0.23013268953278754
1106	44-45	0.21392295854749932
1106	46-47	0.23466217754146612
1106	48-49	0.2885956571087078
1106	50-51	0.2810676616987564
1106	52-53	0.2624498737687304
1106	54-55	0.23432821007673255
1106	56-57	0.19906697263797213
1106	58-59	0.3269954130017254
1106	60-61	0.3559220953071147
1106	62-63	0.35472813502740763
1106	64-65	0.3226638326994973
1106	66-67	0.27446375876933615
1106	68-69	0.2837421201227315
1106	70-71	0.31683900407773535
1106	72-73	0.3170344924057744
1106	74-75	0.28038257937587474
1106	76-77	0.19112006427851114
1106	78-79	0.3155063641514353
1106	80-81	0.3344166791987213
1106	82-83	0.312918495233653
1106	84-85	0.36365827415162144
1106	86-87	0.3823736039534893
1106	88-89	0.3551732158363592
1106	90-91	0.30598742244920274
1106	92-93	0.41724643066574885
1106	94-95	0.40183680898049445
1106	96-97	0.41435658024536437
1106	98-99	0.44751628208351235
1106	100	0.473812863710414
1107	1	0.06759389270713712
1107	2	0.0734936024817543
1107	3	0.09408169258674093
1107	4	0.055220658452796556
1107	5	0.05323610657814015
1107	6	0.0875204089713435
1107	7	0.048380584566956486
1107	8	0.06266584370098371
1107	9	0.08853046167527623
1107	10-11	0.1277387015112197
1107	12-13	0.10613825470074545
1107	14-15	0.17340949512251314
1107	16-17	0.15341545298131365
1107	18-19	0.1469776148658113
1107	20-21	0.17495183054792562
1107	22-23	0.1747055424897752
1107	24-25	0.1488321583218024
1107	26-27	0.20271905314868377
1107	28-29	0.151252156353614
1107	30-31	0.13790477888642982
1107	32-33	0.13244081887710024
1107	34-35	0.15732852793622243
1107	36-37	0.12423698757530843
1107	38-39	0.1565770197572931
1107	40-41	0.16946848012230475
1107	42-43	0.19634396047361946
1107	44-45	0.1668603230083079
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2310	28-29	-2.3361536042187936E-4
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2311	10-11	-0.11264726180673534
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2314	86-87	-0.531591432604408
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2314	92-93	-0.5708892522963609
2314	94-95	-0.519025444235222
2314	96-97	-0.5980394499396837
2314	98-99	-0.6479066826366697
2314	100	-0.713366747358581
2315	1	-0.3467446476032947
2315	2	-0.3327050370444482
2315	3	-0.3139366011576712
2315	4	-0.24784434597740557
2315	5	-0.2921898251897943
2315	6	-0.2712992587499343
2315	7	-0.24384046356499312
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2315	9	-0.3431714848448948
2315	10-11	-0.3335870478858709
2315	12-13	-0.34984450783894516
2315	14-15	-0.5583997710003104
2315	16-17	-0.5018499524635232
2315	18-19	-0.4274368153331878
2315	20-21	-0.4396935917995677
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2315	24-25	-0.5317816825344792
2315	26-27	-0.553340804295992
2315	28-29	-0.5815344191214464
2315	30-31	-0.4757990775235328
2315	32-33	-0.48379782481122646
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2315	96-97	-0.9298217171515226
2315	98-99	-0.9282808150242481
2315	100	-0.9461333125195601
2316	1	-0.4236010161257653
2316	2	-0.37667781582518245
2316	3	-0.41602827605699133
2316	4	-0.2855909732489721
2316	5	-0.3291600575335565
2316	6	-0.3374742407337692
2316	7	-0.3607182265428932
2316	8	-0.3105987969873496
2316	9	-0.407041550723271
2316	10-11	-0.3868572965967303
2316	12-13	-0.43645659976159124
2316	14-15	-0.9353087702379739
2316	16-17	-0.7687676890588975
2316	18-19	-0.7016608877200028
2316	20-21	-0.6732130906051168
2316	22-23	-0.6079670133573458
2316	24-25	-0.6090839484116017
2316	26-27	-0.5757920497575739
2316	28-29	-0.6015445777492587
2316	30-31	-0.7314527005044837
2316	32-33	-0.7298827579605316
2316	34-35	-0.7002987432077177
2316	36-37	-0.7246080235398082
2316	38-39	-0.6162232779954522
2316	40-41	-0.6258476821384846
2316	42-43	-0.6191854391558138
2316	44-45	-0.7583620677026772
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2316	50-51	-0.6498189606057565
2316	52-53	-0.6968799934323187
2316	54-55	-0.787089845563905
2316	56-57	-0.7746918057233287
2316	58-59	-0.727259099783339
2316	60-61	-0.6929157002860933
2316	62-63	-0.7299121651984919
2316	64-65	-0.6522043746833859
2316	66-67	-0.6628542932459851
2316	68-69	-0.5982013224991931
2316	70-71	-0.6256920392247522
2316	72-73	-0.6266761109671037
2316	74-75	-0.6671227262900707
2316	76-77	-0.7275559631955275
2316	78-79	-0.7403943322836319
2316	80-81	-0.7134638254948058
2316	82-83	-0.7292978542598227
2316	84-85	-0.682628551420418
2316	86-87	-0.6963936897255891
2316	88-89	-0.6928924714958384
2316	90-91	-0.7633761851619489
2316	92-93	-0.7580321871150382
2316	94-95	-0.7430248103341697
2316	96-97	-0.7446792615959943
2316	98-99	-0.7649934635487128
2316	100	-0.8077482542693453
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	17.0
3	24.0
4	38.0
5	100.0
6	270.0
7	740.0
8	1831.0
9	3439.0
10	6486.0
11	12943.0
12	22903.0
13	33780.0
14	36556.0
15	45407.0
16	59768.0
17	80234.0
18	81114.0
19	50716.0
20	33058.0
21	27756.0
22	29723.0
23	32845.0
24	35514.0
25	36294.0
26	40521.0
27	49027.0
28	59453.0
29	74589.0
30	90934.0
31	113942.0
32	143804.0
33	187635.0
34	269428.0
35	448148.0
36	902493.0
37	1946460.0
38	1808937.0
39	337376.0
40	380.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.434844332405625	18.156609191559493	13.851177531399358	22.557368944635524
2	14.589699423834194	23.191377846821794	35.98310086365728	26.23582186568673
3	19.913768507911144	28.48918805669062	29.625325687016446	21.971717748381785
4	13.780262961767612	18.95656709806757	33.71670488324391	33.5464650569209
5	13.29029355806689	37.86669774516225	32.25132875347786	16.591679943293006
6	30.589049504390275	33.45580654337428	18.188454009841116	17.766689942394333
7	24.752223287091063	33.56328494881475	21.813499631158773	19.870992132935417
8	28.664150110567917	30.667335896619175	21.399448786103477	19.269065206709435
9	28.89345868847221	14.730185942517645	19.573178842203273	36.803176526806865
10-11	25.061075912887315	26.761996277666434	24.899562725036432	23.27736508440982
12-13	27.760527809615148	24.73358065377442	25.72570795910247	21.780183577507962
14-15	26.607084369563005	23.299104266861733	25.453633891899187	24.640177471676076
16-17	23.112311977888385	26.59440963094342	27.930183514169453	22.363094876998733
18-19	24.934891535625166	25.284907152085463	29.504272041412683	20.275929270876688
20-21	24.682503622980338	25.25446017874496	28.651116846323703	21.411919351951003
22-23	23.088369683826194	26.82255017289958	25.672457433133587	24.41662271014064
24-25	23.171953720700145	27.59365319858922	25.114396948720298	24.11999613199034
26-27	24.247886307271628	25.427656483503075	25.85312936741912	24.471327841806183
28-29	23.61988956062057	24.470055031075283	29.08894796148972	22.821107446814427
30-31	25.753502885441264	24.742993173416725	27.75760998360708	21.745893957534932
32-33	21.468798362731306	26.406552734423112	28.76902223445232	23.355626668393267
34-35	21.113228470104744	27.259243003964677	28.637265530800065	22.99026299513051
36-37	23.866209090539297	27.098168911969754	26.606605811969374	22.429016185521576
38-39	24.679848488665858	25.137286209673253	26.559362324821528	23.62350297683936
40-41	23.08457960740194	26.681876182473935	26.563268984136702	23.670275225987425
42-43	25.754370476864963	25.475083350147752	26.441074491135325	22.32947168185196
44-45	25.358311179562165	24.954553509609177	27.359258746804315	22.32787656402434
46-47	23.572258422851185	25.685385490014696	28.802475937224386	21.939880149909737
48-49	24.489957641563798	26.558650824549595	27.80861458045668	21.14277695342993
50-51	22.891886354904774	27.534118421044298	26.09305028399732	23.48094494005361
52-53	22.36127784562114	28.146313341919342	24.079826676969503	25.412582135490013
54-55	23.467311877660833	27.056993606538775	23.958666164821295	25.517028350979093
56-57	24.196139345678894	25.09953287920082	24.695804782857994	26.00852299226229
58-59	23.704917656616644	25.03933439717278	27.04028730563057	24.215460640580005
60-61	24.105448588132788	25.818128679916093	25.941262404703874	24.13516032724725
62-63	21.07423334154984	26.653682146780213	26.98462058675427	25.28746392491568
64-65	20.937118288457167	26.692902568704913	26.438761328361092	25.931217814476824
66-67	23.34769850522217	26.52138219532716	25.005090035389205	25.125829264061466
68-69	23.858935558269884	25.221515896137227	25.18563110917561	25.73391743641728
70-71	22.46207184754056	26.124656089511667	25.454471367688043	25.958800695259733
72-73	24.082932543939275	25.347190894361493	25.622937844789256	24.946938716909976
74-75	24.230939786657835	24.66748111592263	26.225007993064768	24.876571104354767
76-77	22.658368503500785	25.833919864448852	27.10528191884033	24.402429713210037
78-79	22.843765786691524	25.73841072979152	26.980401524052215	24.43742195946474
80-81	21.88889161014661	25.97775259206836	26.496475924329143	25.636879873455886
82-83	21.816403066531507	26.762364640019232	24.628232459820953	26.792999833628308
84-85	22.131285587553343	26.114093418900055	24.72819123648416	27.026429757062438
86-87	22.413725638952958	24.519239633979584	25.592426967705993	27.474607759361465
88-89	22.612084686207357	23.979432842929988	26.709899519017743	26.698582951844912
90-91	23.093274791326575	24.63187448431603	26.090688313359067	26.18416241099833
92-93	21.243470369508906	25.288971667429667	26.60127036831251	26.86628759474892
94-95	20.676400619566017	25.51707504823777	26.92516683262604	26.881357499570175
96-97	22.130510348252237	25.060713518614076	25.84115001411746	26.96762611901623
98-99	22.519350968931338	24.332056757493614	25.790960694516563	27.357631579058488
100	22.363874446142432	25.18000942784203	26.123497411284614	26.332618714730927
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	549.0
1	354.5
2	215.5
3	277.0
4	236.5
5	300.5
6	510.0
7	961.5
8	1543.5
9	1691.5
10	1409.0
11	1317.5
12	1522.0
13	1706.5
14	1968.5
15	2396.0
16	2910.5
17	3578.0
18	4350.5
19	5192.5
20	6233.0
21	7718.5
22	9685.0
23	11866.0
24	14499.0
25	18135.5
26	23213.5
27	29397.0
28	35421.5
29	43123.0
30	52334.5
31	61625.5
32	71511.5
33	81508.5
34	91148.0
35	98260.5
36	104686.0
37	113636.0
38	119134.0
39	121816.5
40	122746.5
41	122531.0
42	125396.0
43	136839.5
44	154495.5
45	172925.5
46	202421.5
47	249188.5
48	385860.0
49	540899.5
50	706672.5
51	681208.5
52	431222.0
53	298391.0
54	246903.5
55	205935.5
56	179258.0
57	158992.0
58	148537.0
59	136533.0
60	114182.5
61	92507.0
62	75285.5
63	58536.0
64	42474.5
65	33521.0
66	25931.5
67	18877.0
68	14778.0
69	12399.0
70	10905.5
71	9598.0
72	8320.5
73	9020.0
74	7263.0
75	5255.5
76	3957.0
77	2516.0
78	1726.0
79	958.0
80	593.0
81	452.5
82	344.0
83	251.5
84	179.0
85	98.0
86	60.5
87	31.5
88	11.5
89	8.0
90	6.0
91	3.0
92	2.0
93	1.5
94	1.0
95	0.5
96	1.0
97	0.5
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01120387778033165
2	0.04581485197861748
3	0.001210469207422766
4	0.0
5	0.0012949205474755171
6	0.0
7	0.0
8	0.0
9	9.571151872645126E-4
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	4.926328169743816E-4
22-23	0.001766440529436711
24-25	0.003575106728899797
26-27	0.008269193713498546
28-29	0.0034132416604653578
30-31	0.005587863666823699
32-33	0.0023646375214770314
34-35	0.0062634743872457075
36-37	0.0
38-39	0.0
40-41	0.0019634936562264635
42-43	0.0023435246864638437
44-45	0.0024490888615297823
46-47	0.0019846064912396513
48-49	0.0010837921973436396
50-51	0.0017805157527788363
52-53	0.00691797227265453
54-55	0.0033358279320836693
56-57	0.02998726333039771
58-59	0.016432823251931156
60-61	0.02012756937923902
62-63	0.015426444782969204
64-65	0.0023787127448191567
66-67	0.0016256882960154592
68-69	1.4075223342125186E-5
70-71	0.0
72-73	0.0022801861814242806
74-75	0.0012175068190938285
76-77	8.234005655143235E-4
78-79	0.0028431951151092877
80-81	0.0017594029177656484
82-83	0.0015341993442916453
84-85	0.00401847626417674
86-87	7.319116137905098E-4
88-89	7.741372838168853E-4
90-91	5.770841570271327E-4
92-93	7.107987787773219E-4
94-95	4.574447586190686E-4
96-97	7.037611671062594E-5
98-99	0.0
100	0.0027305933283722863
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7104683.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.91157707984937
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.05898948714474	26.019560263611652
2	11.944520706929621	7.862260278559424
3	3.484870494900153	3.440777517185977
4	1.4802892646206698	1.9487461693214687
5	0.8187249391796945	1.347276447150375
6	0.5381597407071065	1.0627011472532213
7	0.37314990544562265	0.859666631278848
8	0.2779909730211844	0.7319297068871231
9	0.21590116931444864	0.6395083177969878
>10	1.4341759161578305	9.26589338257829
>50	0.17469293359349358	4.01846269482913
>100	0.1615858164418236	11.117270885359325
>500	0.021962488094198013	4.904036617981002
>1k	0.012445406683522824	7.719234991696997
>5k	7.751464370358575E-4	1.8391231923624887
>10k+	0.0017656113288038976	17.223551756147558
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	135322	1.9046873731030645	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	107706	1.5159860052869354	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	75893	1.068210925103907	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	60481	0.8512835829550733	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	56003	0.7882547328290369	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	55512	0.7813437981680533	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	54664	0.7694080087739312	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	52863	0.7440585315347638	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	48387	0.6810578318554114	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	42249	0.594664110981447	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	38410	0.5406293285710284	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	36966	0.5203047060649997	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	25067	0.3528236235170521	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	23456	0.3301484387128884	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	23107	0.3252361857664867	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22100	0.31106243586096666	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC	21231	0.2988310667766599	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC	20817	0.29300392431302	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	19470	0.27404459847117735	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT	19228	0.2706383944223831	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18032	0.25380442730520136	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG	16964	0.23877208877581169	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	15711	0.2211358339281288	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA	15399	0.21674436424538576	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	15217	0.21418267359711896	No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG	15105	0.21260624858280094	No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	15030	0.21155060683214155	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	14501	0.20410481368415734	No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	14119	0.19872807836746553	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	13663	0.19230977652345643	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13308	0.187313072237002	No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA	11635	0.16376522358562656	No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	11427	0.1608375771304645	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC	11344	0.15966933359306812	No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	11233	0.15810698380209223	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	11081	0.1559675498540892	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	11000	0.15482745676337706	No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC	10756	0.1513931022678985	No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC	10511	0.14794467254907784	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG	10202	0.14359542853636115	No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	10072	0.1417656495018849	No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA	9296	0.13084327618839575	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	8971	0.12626882860220506	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	8713	0.12263742097993674	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT	8710	0.12259519530991038	No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC	8260	0.11626134480595404	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	8153	0.11475529590834666	No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT	7988	0.11243288405689598	No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA	7836	0.11029345010889298	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	7799	0.10977266684523435	No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA	7669	0.10794288781075807	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.007276890467878721	0.0	0.0	5.630089336850075E-5	0.0
2	0.010753470633383643	0.0	0.0	7.037611671062594E-5	0.0
3	0.011330554790410776	0.0	0.0	7.037611671062594E-5	0.0
4	0.013526289631782304	0.0	0.0	7.037611671062594E-5	0.0
5	0.05399255674039222	0.0	0.0	3.096549135267541E-4	0.0
6	0.12016018167172272	0.0	0.0	7.178363904483846E-4	0.0
7	0.13779643651940557	0.0	0.0	9.00814293896012E-4	0.0
8	0.15793808112198673	0.0	0.0	0.0010274913039751386	0.0
9	0.1729704196513764	0.0	0.0	0.001210469207422766	0.0
10-11	0.31403230798615506	0.0	0.0	0.002216847676384717	0.0
12-13	0.5287005767885773	0.0	0.0	0.004623710867888124	0.0
14-15	0.7643901916524636	0.0	7.037611671062594E-6	0.007171326292812783	0.0
16-17	0.8579059755375433	0.0	1.4075223342125188E-5	0.008311419383524924	0.0
18-19	0.8983722426461533	0.0	1.4075223342125188E-5	0.009071481443999684	0.0
20-21	1.0484633867549054	0.0	1.4075223342125188E-5	0.01164724731560859	0.0
22-23	1.0937926435282193	0.0	1.4075223342125188E-5	0.014877511072626321	0.0
24-25	1.291507305815052	0.0	1.4075223342125188E-5	0.029790210203607958	0.0
26-27	1.7155726722782705	0.0	1.4075223342125188E-5	0.0787156865408351	0.0
28-29	1.819412632484799	0.0	1.4075223342125188E-5	0.13346126773003103	0.0
30-31	1.8548892329186257	0.0	1.4075223342125188E-5	0.25029265908134113	0.0
32-33	1.8866570120018022	0.0	1.4075223342125188E-5	0.4258036565459712	0.0
34-35	1.910247086323204	0.0	1.4075223342125188E-5	0.6429421270449365	0.0
36-37	1.9476449547432306	0.0	1.4075223342125188E-5	0.9592827716592001	0.0
38-39	1.985802885223732	0.0	1.4075223342125188E-5	1.4275373018050206	0.0
40-41	2.0212724480458877	0.0	1.4075223342125188E-5	2.1337334825494674	0.0
42-43	2.096511554421218	0.0	1.4075223342125188E-5	2.6336783780500834	0.0
44-45	2.180709540453811	0.0	1.4075223342125188E-5	3.1194635988685206	0.0
46-47	2.2139833684345946	0.0	1.4075223342125188E-5	3.6667223576336903	0.0
48-49	2.2342235396005705	0.0	1.4075223342125188E-5	4.150438520620836	0.0
50-51	2.2722266426243083	0.0	1.4075223342125188E-5	4.698379927718099	0.0
52-53	2.2846269143887206	0.0	1.4075223342125188E-5	5.3713656189868	7.037611671062594E-6
54-55	2.3254943253625813	0.0	1.4075223342125188E-5	5.931960651868634	1.4075223342125188E-5
56-57	2.405659759907655	0.0	1.4075223342125188E-5	6.4986432188459355	1.4075223342125188E-5
58-59	2.4310092371468226	1.4075223342125188E-5	1.4075223342125188E-5	7.07752900446086	1.4075223342125188E-5
60-61	2.4495322310650596	2.8150446684250376E-5	1.4075223342125188E-5	7.578972911247412	1.4075223342125188E-5
62-63	2.464979788683042	2.8150446684250376E-5	1.4075223342125188E-5	8.10536515140788	1.4075223342125188E-5
64-65	2.476563697493611	4.222567002637556E-5	1.4075223342125188E-5	8.679465361086482	1.4075223342125188E-5
66-67	2.4934117398341344	4.222567002637556E-5	1.4075223342125188E-5	9.243114717433556	1.4075223342125188E-5
68-69	2.5128144352112542	4.222567002637556E-5	1.4075223342125188E-5	9.92119563955211	1.4075223342125188E-5
70-71	2.5366508259411433	5.630089336850075E-5	1.4075223342125188E-5	10.761296457561865	1.4075223342125188E-5
72-73	2.595182642209371	5.630089336850075E-5	1.4075223342125188E-5	11.426069256010436	2.8150446684250376E-5
74-75	2.65233508659007	5.630089336850075E-5	1.4075223342125188E-5	12.064985869179527	2.8150446684250376E-5
76-77	2.6704076733613586	7.037611671062594E-5	1.4075223342125188E-5	12.735684336655133	2.8150446684250376E-5
78-79	2.6836172704679435	7.037611671062594E-5	1.4075223342125188E-5	13.358935226244437	2.8150446684250376E-5
80-81	2.7096142079808487	7.037611671062594E-5	1.4075223342125188E-5	14.07265461386525	2.8150446684250376E-5
82-83	2.7192838864168887	7.037611671062594E-5	1.4075223342125188E-5	14.929863865847357	2.8150446684250376E-5
84-85	2.755105329822597	7.741372838168853E-5	2.8150446684250376E-5	15.70041900532367	2.8150446684250376E-5
86-87	2.816804071342803	8.445134005275112E-5	2.8150446684250376E-5	16.449101247726325	2.8150446684250376E-5
88	2.8321742152324036	8.445134005275112E-5	2.8150446684250376E-5	17.03971873199691	2.8150446684250376E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCTAC	3830	0.0	32.288277	1
TCCGATC	2950	0.0	29.949623	3
TATCACG	420	0.0	29.104282	2
ATCTACA	4540	0.0	28.063787	2
CGATCTC	2995	0.0	26.51791	5
TCTACAC	5470	0.0	25.860369	3
TACACTC	5460	0.0	24.013803	5
TCTCGCG	1300	0.0	23.858038	8
CCGATCT	3820	0.0	23.005285	4
AGTACTC	3270	0.0	22.706945	5
CTACACT	7125	0.0	20.908545	4
CGCTATA	210	9.5136966E-7	20.149118	2
TCACGCA	575	0.0	19.615204	4
CTCGCGC	1540	0.0	19.5296	9
ATTGCGT	1000	0.0	19.267172	6
TATACGA	175	1.07964144E-4	18.797901	5
GTAAGAA	2775	0.0	18.628553	4
CACTCTT	7280	0.0	18.526129	7
GATGTAA	2525	0.0	18.435787	1
AAGTACT	4380	0.0	18.34727	4
>>END_MODULE