##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765554_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7021801
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.334401387906034	34.0	31.0	34.0	31.0	34.0
2	32.377935945493185	34.0	31.0	34.0	31.0	34.0
3	32.406924092551186	34.0	31.0	34.0	31.0	34.0
4	35.855770051016826	37.0	35.0	37.0	35.0	37.0
5	35.873399431285506	37.0	37.0	37.0	35.0	37.0
6	35.96348728766309	37.0	37.0	37.0	35.0	37.0
7	35.919685846978574	37.0	37.0	37.0	35.0	37.0
8	35.94685109988164	37.0	37.0	37.0	35.0	37.0
9	37.66265193217524	39.0	38.0	39.0	35.0	39.0
10-11	37.595422385225675	39.0	38.0	39.0	35.0	39.0
12-13	37.57123001634481	39.0	38.0	39.0	35.0	39.0
14-15	38.97055264026993	41.0	39.0	41.0	36.0	41.0
16-17	38.93228368334563	41.0	39.0	41.0	36.0	41.0
18-19	38.931042976011426	41.0	39.0	41.0	36.0	41.0
20-21	38.90980796806973	41.0	39.0	41.0	35.5	41.0
22-23	38.81708674170629	41.0	39.0	41.0	35.0	41.0
24-25	38.739003497820576	41.0	39.0	41.0	35.0	41.0
26-27	38.61215448857067	40.5	38.0	41.0	35.0	41.0
28-29	38.46816941978276	40.0	38.0	41.0	34.5	41.0
30-31	38.24358444507328	40.0	38.0	41.0	34.0	41.0
32-33	38.00638803349739	40.0	38.0	41.0	34.0	41.0
34-35	37.85851663981933	40.0	38.0	41.0	33.5	41.0
36-37	37.699689153822504	40.0	37.5	41.0	33.0	41.0
38-39	37.503964296339355	40.0	37.0	41.0	33.0	41.0
40-41	37.256555476294466	40.0	37.0	41.0	32.5	41.0
42-43	36.98779237691299	40.0	36.5	41.0	31.5	41.0
44-45	36.61272364454646	40.0	36.0	41.0	31.0	41.0
46-47	36.1717154900858	40.0	35.5	41.0	30.0	41.0
48-49	35.779422259332044	39.0	35.0	41.0	28.5	41.0
50-51	34.50009128712135	38.0	34.0	40.0	26.0	40.5
52-53	34.42473213068841	38.0	34.5	39.5	26.0	40.5
54-55	35.23316361429212	39.0	35.0	40.5	27.0	41.0
56-57	35.242195129141365	39.0	35.0	41.0	27.0	41.0
58-59	35.024542421524046	38.5	35.0	41.0	26.5	41.0
60-61	34.686062165532746	38.0	35.0	40.5	26.0	41.0
62-63	34.32676353260367	37.0	35.0	40.0	26.0	41.0
64-65	34.01384516593392	37.0	34.5	40.0	25.0	41.0
66-67	33.69261689415579	36.0	34.0	39.0	24.5	41.0
68-69	33.43965785985675	36.0	34.0	39.0	24.0	41.0
70-71	33.094012348114106	35.0	34.0	39.0	24.0	41.0
72-73	32.713279470608754	35.0	34.0	37.0	23.0	39.5
74-75	32.341586937596205	35.0	34.0	37.0	21.0	39.0
76-77	31.985404171949618	35.0	34.0	36.5	20.0	39.0
78-79	31.64266425380041	35.0	33.5	36.0	18.5	37.5
80-81	31.313414250845334	35.0	33.0	35.5	15.0	37.0
82-83	31.035133294150604	35.0	33.0	35.0	8.5	37.0
84-85	30.76831200428494	35.0	33.0	35.0	4.0	36.0
86-87	30.518015534760956	35.0	33.0	35.0	2.0	36.0
88-89	30.279854271005405	35.0	32.5	35.0	2.0	36.0
90-91	30.03082314636943	35.0	32.0	35.0	2.0	35.5
92-93	29.751530056177895	35.0	32.0	35.0	2.0	35.0
94-95	29.41890698696816	35.0	31.5	35.0	2.0	35.0
96-97	29.102748639558428	35.0	31.0	35.0	2.0	35.0
98-99	28.780866119674997	35.0	31.0	35.0	2.0	35.0
100	28.22052889280115	34.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4252208920204694
1101	2	0.5264783580846739
1101	3	0.5038417813355096
1101	4	0.4605972617126284
1101	5	0.5076159659090607
1101	6	0.4455698325860098
1101	7	0.4474170341505257
1101	8	0.41786948860842443
1101	9	0.48253350068857515
1101	10-11	0.4842591616500158
1101	12-13	0.5527693929357156
1101	14-15	0.7330929325881002
1101	16-17	0.7100617673650191
1101	18-19	0.6661738342936872
1101	20-21	0.6693480850313662
1101	22-23	0.728745829497143
1101	24-25	0.6008693302874875
1101	26-27	0.7164569276965622
1101	28-29	0.7181396375566109
1101	30-31	0.7089537132155499
1101	32-33	0.6605734885998658
1101	34-35	0.7674575392743535
1101	36-37	0.7711829168388462
1101	38-39	0.815867464292495
1101	40-41	0.8051817897502502
1101	42-43	0.7286253274572587
1101	44-45	0.7040117251882094
1101	46-47	0.8821246745612186
1101	48-49	0.9434921486761425
1101	50-51	0.9043780384012905
1101	52-53	0.9560981937626991
1101	54-55	1.0292182879438556
1101	56-57	1.0738191559734318
1101	58-59	1.053643139018014
1101	60-61	1.0823509154166757
1101	62-63	1.0748650076235506
1101	64-65	1.0455815373442903
1101	66-67	0.946903889845153
1101	68-69	0.9510408102776609
1101	70-71	0.8732315527208385
1101	72-73	0.8586134308294575
1101	74-75	0.816395038690672
1101	76-77	0.7735164945001074
1101	78-79	0.7854480079513628
1101	80-81	0.7722853911500174
1101	82-83	0.7717047155238355
1101	84-85	0.7697495768960572
1101	86-87	0.7335497819481063
1101	88-89	0.6589554027871145
1101	90-91	0.7105141016842431
1101	92-93	0.6984467212138306
1101	94-95	0.606304846312085
1101	96-97	0.46906487213243153
1101	98-99	0.397773340886058
1101	100	0.4086756537895049
1102	1	0.4303629157840092
1102	2	0.5032920637839808
1102	3	0.6083222109232196
1102	4	0.4882992567983635
1102	5	0.4877629725727317
1102	6	0.45612870906917635
1102	7	0.48615172150501706
1102	8	0.4365979183291273
1102	9	0.4817702734546643
1102	10-11	0.4803614886108818
1102	12-13	0.5004253725228551
1102	14-15	0.6680407968866504
1102	16-17	0.7044124670859944
1102	18-19	0.687589178729354
1102	20-21	0.6207490329591963
1102	22-23	0.6850833131350456
1102	24-25	0.696637527254488
1102	26-27	0.6925211986320292
1102	28-29	0.6160496339537858
1102	30-31	0.6199905695593984
1102	32-33	0.71263938829766
1102	34-35	0.7872457343354355
1102	36-37	0.7933900280586599
1102	38-39	0.724587745263868
1102	40-41	0.7418187707859403
1102	42-43	0.7890197394330656
1102	44-45	0.8055370769167709
1102	46-47	0.8065705275127542
1102	48-49	0.7867935261620502
1102	50-51	0.7953841299658464
1102	52-53	0.848465559330144
1102	54-55	1.0065662296645783
1102	56-57	0.9493235810027159
1102	58-59	0.9551189826917224
1102	60-61	0.9512335393567497
1102	62-63	0.9804341041953677
1102	64-65	0.9815803779867878
1102	66-67	0.9478797482494841
1102	68-69	0.916813676604761
1102	70-71	0.9115781581520039
1102	72-73	0.9032437407864151
1102	74-75	0.8313544866890297
1102	76-77	0.8416991258960209
1102	78-79	0.8362174483803209
1102	80-81	0.810316251061149
1102	82-83	0.7641663735268729
1102	84-85	0.824709574618165
1102	86-87	0.7564289057608704
1102	88-89	0.7326958071039904
1102	90-91	0.7856660945398097
1102	92-93	0.8238169823770498
1102	94-95	0.7863917454570348
1102	96-97	0.8702797945316796
1102	98-99	0.8490058165899974
1102	100	0.96583390460804
1103	1	0.5394000509947148
1103	2	0.5472172444475447
1103	3	0.5628752672947925
1103	4	0.4666885185391081
1103	5	0.47915181717202415
1103	6	0.4512212994113298
1103	7	0.452723274973188
1103	8	0.44756971014047764
1103	9	0.5269259695295574
1103	10-11	0.5218124642398791
1103	12-13	0.5013021021766093
1103	14-15	0.665767506444233
1103	16-17	0.6259135952733104
1103	18-19	0.5963149371968726
1103	20-21	0.6538041839518556
1103	22-23	0.7037401038171609
1103	24-25	0.6331650703655285
1103	26-27	0.605358144429637
1103	28-29	0.646703100961453
1103	30-31	0.6945546752902771
1103	32-33	0.7192114492402482
1103	34-35	0.6414131824676303
1103	36-37	0.6045559995786576
1103	38-39	0.6365947628741466
1103	40-41	0.6512631054802185
1103	42-43	0.6640149248717293
1103	44-45	0.7241161083065748
1103	46-47	0.7510543985200471
1103	48-49	0.8075977605487026
1103	50-51	0.7272411319761503
1103	52-53	0.7633415681438436
1103	54-55	0.8501237366245036
1103	56-57	0.9011209475374926
1103	58-59	0.882925590543131
1103	60-61	0.8153172702457638
1103	62-63	0.7705143093634348
1103	64-65	0.7405083040114846
1103	66-67	0.7587923755869639
1103	68-69	0.738593928297604
1103	70-71	0.7360607111092747
1103	72-73	0.6558742205864903
1103	74-75	0.7239267433897112
1103	76-77	0.7465932638716453
1103	78-79	0.7547626324213859
1103	80-81	0.739710870241371
1103	82-83	0.6332582229060755
1103	84-85	0.7476768919766563
1103	86-87	0.7825241268893919
1103	88-89	0.7912378859298634
1103	90-91	0.6934732582863674
1103	92-93	0.7402356788287143
1103	94-95	0.6365625653071092
1103	96-97	0.6984232220195459
1103	98-99	0.7616585120730583
1103	100	0.8313079707631488
1104	1	0.45345409610122545
1104	2	0.4706577508995764
1104	3	0.5053286707746807
1104	4	0.4535284760203879
1104	5	0.4876199526732492
1104	6	0.41145351683733367
1104	7	0.39141950478696685
1104	8	0.4065463169045245
1104	9	0.44512093196524205
1104	10-11	0.4534630225067673
1104	12-13	0.43978190708088505
1104	14-15	0.5571686025297069
1104	16-17	0.5244492805393861
1104	18-19	0.5179207847160328
1104	20-21	0.5275477914605418
1104	22-23	0.4848933269802842
1104	24-25	0.48157438146288456
1104	26-27	0.4969413667528997
1104	28-29	0.5352478507933682
1104	30-31	0.5388742261528137
1104	32-33	0.5448676935228889
1104	34-35	0.531374810719548
1104	36-37	0.5393260060389693
1104	38-39	0.5924070626221933
1104	40-41	0.552192104931521
1104	42-43	0.4970615001697283
1104	44-45	0.49560676436220064
1104	46-47	0.4892412770584471
1104	48-49	0.4471950272325671
1104	50-51	0.4354744335776459
1104	52-53	0.49263882152425253
1104	54-55	0.5369566307321705
1104	56-57	0.545508013546204
1104	58-59	0.5210575853884976
1104	60-61	0.480156017469767
1104	62-63	0.47646384016255183
1104	64-65	0.4909238226738708
1104	66-67	0.5080073098279172
1104	68-69	0.48734754685838055
1104	70-71	0.40683488856734584
1104	72-73	0.40245129896745624
1104	74-75	0.3647687114888001
1104	76-77	0.31518413450778837
1104	78-79	0.30187923269049577
1104	80-81	0.27338823854938354
1104	82-83	0.31549397498620024
1104	84-85	0.2990278218183029
1104	86-87	0.26647972317150703
1104	88-89	0.30782669015103536
1104	90-91	0.2290695194576884
1104	92-93	0.2550619706020747
1104	94-95	0.24739726842970455
1104	96-97	0.2553809664923463
1104	98-99	0.2042491829681694
1104	100	0.1953384504421436
1105	1	0.3482476833029935
1105	2	0.376422690783194
1105	3	0.3785131810223632
1105	4	0.3792427252028503
1105	5	0.37887804033549344
1105	6	0.3868585593999825
1105	7	0.3438541956850969
1105	8	0.3329552180016506
1105	9	0.3584839293173232
1105	10-11	0.4267849282723404
1105	12-13	0.40469937004354506
1105	14-15	0.521396882029741
1105	16-17	0.5202309641891176
1105	18-19	0.5192834859791731
1105	20-21	0.4848187998333273
1105	22-23	0.5359006700018298
1105	24-25	0.5425201605457204
1105	26-27	0.4688112267877429
1105	28-29	0.4537895778958969
1105	30-31	0.4890662837360864
1105	32-33	0.535821368046598
1105	34-35	0.49043553513198646
1105	36-37	0.516869411480755
1105	38-39	0.5163109371684058
1105	40-41	0.5004162198486952
1105	42-43	0.4233535560332129
1105	44-45	0.4677961193677973
1105	46-47	0.4938168920261603
1105	48-49	0.6639648754877783
1105	50-51	0.5583711962566795
1105	52-53	0.5225387322088437
1105	54-55	0.5271532416603435
1105	56-57	0.4804405406412613
1105	58-59	0.4358337417012663
1105	60-61	0.430345486124061
1105	62-63	0.44803861512848897
1105	64-65	0.4663603286489746
1105	66-67	0.4336886954628696
1105	68-69	0.4164112889581517
1105	70-71	0.3862514633820666
1105	72-73	0.37126226015463004
1105	74-75	0.41767860030196147
1105	76-77	0.41526408486164
1105	78-79	0.39799344577589224
1105	80-81	0.4909281875067997
1105	82-83	0.4205036820169177
1105	84-85	0.3894534842756947
1105	86-87	0.4223842869011776
1105	88-89	0.4858903461557098
1105	90-91	0.41486824803466504
1105	92-93	0.42325437061083093
1105	94-95	0.36883213159728
1105	96-97	0.3483809753950915
1105	98-99	0.39630041280057426
1105	100	0.5261170304151257
1106	1	0.29491299404429583
1106	2	0.2993166026122367
1106	3	0.30053416956737067
1106	4	0.307153722029895
1106	5	0.27583277281394913
1106	6	0.3003143508103818
1106	7	0.32380620907714075
1106	8	0.2913344088951675
1106	9	0.3920068472519489
1106	10-11	0.3746260457920556
1106	12-13	0.38845596754947564
1106	14-15	0.45534332197872374
1106	16-17	0.4462302416198085
1106	18-19	0.43206650377301514
1106	20-21	0.4638340378463326
1106	22-23	0.4712890094272453
1106	24-25	0.5719612459834664
1106	26-27	0.5161417644395456
1106	28-29	0.5352558951483033
1106	30-31	0.583924583814678
1106	32-33	0.48806059321405826
1106	34-35	0.5220763572714091
1106	36-37	0.5300741770153294
1106	38-39	0.5570952995425742
1106	40-41	0.5918204031991365
1106	42-43	0.631287800679246
1106	44-45	0.5999530472463732
1106	46-47	0.5967673785276375
1106	48-49	0.5415274015912388
1106	50-51	0.534206085060724
1106	52-53	0.5962522472571266
1106	54-55	0.5696825441276303
1106	56-57	0.6204902667141994
1106	58-59	0.6012836087781963
1106	60-61	0.6303626753062517
1106	62-63	0.5679718250877599
1106	64-65	0.6690314226520684
1106	66-67	0.6270478382730857
1106	68-69	0.6435331304549905
1106	70-71	0.6329631832231968
1106	72-73	0.5647162806755759
1106	74-75	0.6483731012768814
1106	76-77	0.6658933839399239
1106	78-79	0.6095692419956826
1106	80-81	0.6470700314489406
1106	82-83	0.6187370977194142
1106	84-85	0.5659270414830395
1106	86-87	0.5601344673380382
1106	88-89	0.5986647608980178
1106	90-91	0.5896848173910456
1106	92-93	0.6142865801553512
1106	94-95	0.5166347686513362
1106	96-97	0.4143515386003038
1106	98-99	0.4890291977697814
1106	100	0.5397271352853323
1107	1	0.2540158784445552
1107	2	0.3050977818001499
1107	3	0.31224630894563177
1107	4	0.24433251303995718
1107	5	0.3145430051062661
1107	6	0.28575283178183497
1107	7	0.29191208802181734
1107	8	0.30773919335393884
1107	9	0.33865419984807943
1107	10-11	0.2875276085699241
1107	12-13	0.2993220190533492
1107	14-15	0.32881360169900375
1107	16-17	0.3836524827810308
1107	18-19	0.4209202560246581
1107	20-21	0.41235555772821186
1107	22-23	0.3599510133124042
1107	24-25	0.38778497695922454
1107	26-27	0.4120485391660438
1107	28-29	0.4279623670500641
1107	30-31	0.4389180413159579
1107	32-33	0.40738317613769226
1107	34-35	0.4335848939818234
1107	36-37	0.5072799689418233
1107	38-39	0.5436240176968496
1107	40-41	0.5189886064581373
1107	42-43	0.5630172433038965
1107	44-45	0.5184467926570377
1107	46-47	0.5767558388470562
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2316	94-95	-0.5642110424717082
2316	96-97	-0.8547510463917938
2316	98-99	-0.6451289756171157
2316	100	-0.7400639329972947
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	20082.0
3	2775.0
4	2821.0
5	3227.0
6	3841.0
7	4585.0
8	5816.0
9	7078.0
10	9266.0
11	13927.0
12	21121.0
13	30996.0
14	32884.0
15	40653.0
16	54134.0
17	76225.0
18	77014.0
19	43557.0
20	35598.0
21	31417.0
22	32540.0
23	37054.0
24	41574.0
25	44966.0
26	50445.0
27	59031.0
28	70598.0
29	84974.0
30	103872.0
31	127528.0
32	162298.0
33	217879.0
34	315264.0
35	512413.0
36	941723.0
37	1733670.0
38	1607085.0
39	361219.0
40	651.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.47136686635885	18.060000590110853	13.692168245450764	23.77646429807953
2	15.141001121027717	21.728656447780377	37.44908136141811	25.681261069773797
3	18.465332491963615	26.97697364568375	29.797271414006747	24.76042244834589
4	13.10871839526721	18.142592189457844	35.34836270471548	33.40032671055947
5	13.74899394660548	36.46647615055145	33.3291755270091	16.455354375833974
6	30.657154861199782	35.01392032424405	18.170163926708405	16.158760887847766
7	25.784826210505063	32.52681271657248	21.98445137111739	19.703909701805063
8	27.33835525520955	31.18970937259164	21.17893873803994	20.29299663415887
9	27.70316410525221	14.645751592199433	19.72791717466107	37.92316712788729
10-11	24.765558485497074	25.655027732304486	26.434628335447226	23.14478544675121
12-13	26.93849740592369	23.786937390961707	26.669288820487637	22.605276382626972
14-15	25.112472561730893	23.284957048538516	25.581935969656467	26.020634420074128
16-17	22.773187057943066	26.56512948697128	26.95382683732034	23.70785661776532
18-19	23.73279989453731	25.452425228067344	29.079702513042307	21.73507236435304
20-21	24.42843412454021	24.718649319785392	28.248934848865105	22.603981706809297
22-23	23.330866902695497	25.52048094470119	26.85441499049605	24.294237162107265
24-25	23.050711892946712	26.234298577641102	26.579403444056382	24.135586085355804
26-27	23.676401411476995	25.207894048327184	26.873179760971876	24.242524779223945
28-29	23.073197030564494	24.62408161494173	28.8326161156166	23.470105238877178
30-31	24.216403841929193	24.68930916826478	28.02856851362096	23.06571847618506
32-33	22.247622658353183	25.56918130746457	28.02021078523771	24.162985248944537
34-35	22.234931165595533	25.935109361812774	28.37610370611902	23.453855766472667
36-37	23.276012689976312	25.925629310690333	27.30517145203184	23.493186547301512
38-39	23.72403057249318	24.87678332236292	27.243192276332724	24.155993828811177
40-41	23.04369772183556	25.61562961762756	27.289407669831927	24.051264990704958
42-43	24.44468035366325	25.12547490890383	26.999764938406663	23.43007979902626
44-45	24.049831308428374	25.026370647987413	27.364999502642412	23.5587985409418
46-47	23.187980154728386	25.217938434733494	28.179851859018967	23.414229551519153
48-49	23.61492892736921	25.94168506377991	27.405559622131452	23.037826386719427
50-51	22.867224290535308	26.403140505657944	26.279212989624657	24.450422214182087
52-53	22.620386041421746	26.49302280738961	25.46513072350784	25.42146042768081
54-55	23.23874511310904	25.986593227654286	25.434211344029713	25.340450315206965
56-57	23.707385059225007	24.977926443151468	25.77872029267141	25.53596820495212
58-59	23.41241803479763	24.84495498260575	27.14925824843486	24.59336873416176
60-61	23.342141972177856	25.328263553637708	26.504675261232723	24.824919212951706
62-63	22.055373291837725	25.516712113548483	26.940803151547943	25.487111443065846
64-65	22.19412870727104	25.502001074787255	26.865154407632957	25.43871581030875
66-67	23.43160431753821	25.40109489100532	26.023848282302133	25.143452509154336
68-69	23.629624476082682	24.799047706896285	26.05307755223776	25.518250264783276
70-71	22.93615170143031	25.29498011338377	26.280102210669003	25.488765974516923
72-73	23.96815053083518	25.025919665831907	26.22164411380561	24.784285689527298
74-75	24.171278128968734	24.776759086174174	26.4004908745844	24.651471910272694
76-77	23.492033033467894	25.57767551615685	26.69882890549709	24.231462544878166
78-79	23.632400608605238	25.528569540797584	26.602911624091796	24.23611822650538
80-81	23.24261785054139	25.490656475636168	26.344606494336976	24.92211917948547
82-83	23.410195922942968	25.893491056745916	25.412326729354785	25.28398629095634
84-85	23.681958613231522	25.822681330157526	25.27200861148383	25.22335144512711
86-87	23.765906804316003	24.999425676307172	25.788603505900127	25.4460640134767
88-89	24.047851637383538	24.48260648904553	26.305418332689573	25.164123540881356
90-91	24.434029082343333	25.059954236543724	25.678268079236677	24.827748601876262
92-93	23.95029276936282	25.20378925054793	25.898925322869065	24.946992657220182
94-95	23.661891926776427	25.305325731523187	26.165382109026332	24.867400232674054
96-97	24.690335842512205	25.087460104256014	25.285479071252663	24.936724981979108
98-99	24.896566640985444	24.679021062773607	25.191041483814576	25.233370812426376
100	24.859403067618853	24.95606457623935	25.382408063203492	24.802124292938302
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2981.0
1	3542.5
2	2671.5
3	1100.0
4	853.0
5	866.5
6	1183.0
7	1920.0
8	2981.0
9	3242.5
10	2554.5
11	2185.5
12	2439.0
13	2868.0
14	3200.5
15	3549.5
16	4166.5
17	4827.0
18	5580.0
19	6496.5
20	7716.5
21	9274.0
22	11238.0
23	13758.5
24	17062.0
25	21070.5
26	26016.0
27	31812.5
28	37612.0
29	45481.5
30	54001.5
31	60785.5
32	68259.5
33	76552.5
34	84908.0
35	92060.5
36	98738.5
37	107314.0
38	113532.5
39	119874.0
40	126494.5
41	132805.0
42	142086.0
43	158575.0
44	181156.5
45	202877.0
46	230501.0
47	266178.5
48	358457.0
49	448779.5
50	505059.5
51	469715.0
52	359162.0
53	310204.0
54	278778.5
55	247845.0
56	224697.5
57	199766.0
58	181552.5
59	164975.0
60	140763.5
61	115657.5
62	93592.0
63	72872.5
64	54125.5
65	42417.5
66	32739.5
67	23611.5
68	17861.0
69	14555.0
70	12348.0
71	10498.0
72	8975.5
73	9019.0
74	7259.5
75	5578.0
76	4391.5
77	2933.0
78	2112.5
79	1501.5
80	1124.5
81	874.0
82	648.0
83	497.0
84	369.0
85	240.5
86	165.5
87	124.0
88	91.5
89	65.5
90	63.0
91	59.0
92	46.5
93	36.5
94	31.5
95	26.0
96	22.5
97	20.0
98	16.0
99	12.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08786919481198627
2	0.2875330702194494
3	0.29221847785204963
4	0.30062088059744213
5	0.3263835018964508
6	0.17468452894065212
7	0.1917741616431454
8	0.1576803444016713
9	0.2089207597879803
10-11	0.23017599046170636
12-13	0.24752908833502973
14-15	0.17378020254347853
16-17	0.16324159571027433
18-19	0.18068014174711017
20-21	0.21264487558106532
22-23	0.17583095846777771
24-25	0.20046139160024615
26-27	0.19887347989497284
28-29	0.2282178033812123
30-31	0.2634153260680558
32-33	0.23120136842385594
34-35	0.1565125528336676
36-37	0.15092993948418648
38-39	0.11737017326466528
40-41	0.10794239255712315
42-43	0.12454781899971247
44-45	0.1387606968639527
46-47	0.20635019420231362
48-49	0.2109715157122795
50-51	0.21769343790859355
52-53	0.14483463715363054
54-55	0.08677261004690962
56-57	0.1265344888013773
58-59	0.14652935906329442
60-61	0.12261811464038927
62-63	0.14434331021343386
64-65	0.11848812007062007
66-67	0.12235464946955917
68-69	0.1078640650739034
70-71	0.13417497875545034
72-73	0.1594676351551404
74-75	0.1979691534977992
76-77	0.17867210990456722
78-79	0.20369418045313448
80-81	0.10535046493057834
82-83	0.14769715063129815
84-85	0.17881452351042132
86-87	0.19308436681700322
88-89	0.17400806431284507
90-91	0.1779671625555894
92-93	0.1261428513852785
94-95	0.3666865523531641
96-97	0.3667720005166766
98-99	0.4131917153448239
100	0.44757748047829893
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7021801.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.16959425695882
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.64013820991062	34.380324530899635
2	11.643202849761062	10.05264685751303
3	3.4379725471786937	4.452476397848022
4	1.5600597493970791	2.6938858559233907
5	0.8419705003772322	1.817376243880685
6	0.5330656049438602	1.3807335526660047
7	0.3628280001849555	1.0964196287133814
8	0.2784689158003727	0.9617112086621854
9	0.20548023041349534	0.7983448357299302
>10	1.2374298484014734	10.263466854287898
>50	0.12874647776937803	3.857025191296066
>100	0.10860248883424979	9.766062268258697
>500	0.01311506076575808	3.9275311569060674
>1k	0.007664176936049345	5.928601289608806
>5k	5.946344174063891E-4	1.7832323841777058
>10k+	6.607049082293213E-4	6.840161743628681
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	60957	0.8681106172049023	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	56643	0.8066733876394389	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	34452	0.490643354888582	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	33082	0.47113269088656884	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	31722	0.45176444049040976	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	26299	0.3745335420357256	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26227	0.373508164073576	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	23044	0.32817791333021257	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	21824	0.31080345341601107	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	20979	0.2987695037213387	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	20454	0.2912927894139979	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	18098	0.25774014387476946	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17384	0.24757181241678597	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	16438	0.2340994853029871	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	13972	0.1989802900993634	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	13150	0.1872738916981555	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12766	0.18180520923335763	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	11279	0.1606283060428514	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	10639	0.15151383526818832	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC	10226	0.14563215334641355	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	9133	0.13006634622655927	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	8743	0.12451221559824895	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC	8541	0.1216354607599959	No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	8295	0.11813208605598478	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	8231	0.11722063897851846	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT	7927	0.11289126536055351	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	7490	0.10666779078472886	No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	7187	0.10235265852734932	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7089632702493277E-4	0.0	0.0	0.0	0.0
2	3.4179265404986553E-4	0.0	0.0	0.0	0.0
3	3.5603401463527663E-4	0.0	0.0	1.4241360585411064E-5	0.0
4	3.845167358060987E-4	1.4241360585411064E-5	0.0	7.120680292705532E-5	0.0
5	0.0013814119767848732	1.4241360585411064E-5	0.0	1.4241360585411065E-4	0.0
6	0.0026061689871302246	1.4241360585411064E-5	0.0	1.4241360585411065E-4	0.0
7	0.0029337202805946793	1.4241360585411064E-5	0.0	1.4241360585411065E-4	0.0
8	0.003360961098157011	1.4241360585411064E-5	0.0	1.7089632702493277E-4	0.0
9	0.0035888228675235883	1.4241360585411064E-5	0.0	2.1362040878116596E-4	0.0
10-11	0.006109543691141347	1.4241360585411064E-5	0.0	2.421031299519881E-4	0.0
12-13	0.012981000173602186	1.4241360585411064E-5	0.0	3.1330993287904344E-4	0.0
14-15	0.020806627815285566	1.4241360585411064E-5	0.0	4.0587877668421535E-4	2.1362040878116596E-5
16-17	0.02409638211051552	1.4241360585411064E-5	0.0	5.269303416602093E-4	4.272408175623319E-5
18-19	0.02588367286398461	1.4241360585411064E-5	0.0	6.907059883924367E-4	5.696544234164426E-5
20-21	0.03274088798586004	1.4241360585411064E-5	0.0	0.0011749122482964127	5.696544234164426E-5
22-23	0.035389781054746494	2.1362040878116596E-5	0.0	0.003788201915719343	5.696544234164426E-5
24-25	0.05308467158211975	3.560340146352766E-5	0.0	0.01002591785212939	5.696544234164426E-5
26-27	0.10841235745644173	4.272408175623319E-5	0.0	0.01990942209840467	6.408612263434979E-5
28-29	0.1200831524561861	4.272408175623319E-5	0.0	0.061750539498342374	7.120680292705532E-5
30-31	0.12185620184906978	4.272408175623319E-5	0.0	0.18138508909608803	7.120680292705532E-5
32-33	0.12323049314556195	4.272408175623319E-5	0.0	0.35540739476951855	7.120680292705532E-5
34-35	0.12414906090332095	4.272408175623319E-5	0.0	0.5760915184010484	7.120680292705532E-5
36-37	0.12635647179405968	4.272408175623319E-5	0.0	0.9116891805962601	7.120680292705532E-5
38-39	0.1285923654059692	4.272408175623319E-5	0.0	1.3699405608333246	7.120680292705532E-5
40-41	0.1305007077244143	4.272408175623319E-5	0.0	1.9655498639166789	7.120680292705532E-5
42-43	0.13440996120510962	4.272408175623319E-5	0.0	2.41510689351635	7.120680292705532E-5
44-45	0.13933747196766186	4.272408175623319E-5	0.0	2.8723756198730213	7.120680292705532E-5
46-47	0.14160896898103492	4.272408175623319E-5	0.0	3.3994127717376212	7.120680292705532E-5
48-49	0.14316127728484473	4.272408175623319E-5	0.0	3.879638571357975	7.120680292705532E-5
50-51	0.14536156749529072	6.408612263434979E-5	0.0	4.387179300581147	7.120680292705532E-5
52-53	0.14626589389246436	8.544816351246638E-5	0.0	4.95743898182247	7.120680292705532E-5
54-55	0.14862283906934987	9.256884380517192E-5	0.0	5.4562283949659065	7.120680292705532E-5
56-57	0.15373548751951244	9.968952409787746E-5	0.0	5.976650434838583	7.120680292705532E-5
58-59	0.15575064004234812	9.968952409787746E-5	0.0	6.504136189561624	7.120680292705532E-5
60-61	0.1566620871198144	1.1393088468328852E-4	1.4241360585411064E-5	6.9825462157073375	7.120680292705532E-5
62-63	0.15722462086293815	1.1393088468328852E-4	1.4241360585411064E-5	7.472335088960795	7.120680292705532E-5
64-65	0.15772306848342754	1.1393088468328852E-4	1.4241360585411064E-5	7.984732122143592	7.120680292705532E-5
66-67	0.15853482603679597	1.1393088468328852E-4	1.4241360585411064E-5	8.499849255198203	7.120680292705532E-5
68-69	0.1594462731142623	1.1393088468328852E-4	1.4241360585411064E-5	9.091527942759985	8.544816351246638E-5
70-71	0.160735116247242	1.1393088468328852E-4	1.4241360585411064E-5	9.763171870008849	8.544816351246638E-5
72-73	0.16381125013369077	1.1393088468328852E-4	1.4241360585411064E-5	10.32327034047248	8.544816351246638E-5
74-75	0.16632485027701582	1.1393088468328852E-4	1.4241360585411064E-5	10.85618205357856	8.544816351246638E-5
76-77	0.1670582803471645	1.1393088468328852E-4	1.4241360585411064E-5	11.423144859844362	9.968952409787746E-5
78-79	0.16771338293409338	1.1393088468328852E-4	1.4241360585411064E-5	11.982880175613065	1.0681020439058298E-4
80-81	0.16880996769917006	1.1393088468328852E-4	1.4241360585411064E-5	12.595564870038327	1.1393088468328852E-4
82-83	0.16935826008170837	1.1393088468328852E-4	1.4241360585411064E-5	13.274827925200388	1.2817224526869957E-4
84-85	0.1711953955972264	1.1393088468328852E-4	1.4241360585411064E-5	13.904132287428823	1.2817224526869957E-4
86-87	0.17474861506328648	1.1393088468328852E-4	1.4241360585411064E-5	14.520704873293901	1.4241360585411065E-4
88	0.17548204513343513	1.1393088468328852E-4	1.4241360585411064E-5	15.00249010189836	1.5665496643952172E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCTAC	6950	0.0	28.043423	1
ATCTACA	7965	0.0	24.864494	2
TCTACAC	9165	0.0	23.864769	3
TACACTC	9445	0.0	22.013765	5
GTACTAG	1845	0.0	21.178596	1
AGTACTT	30170	0.0	20.72657	12-13
GTATAAG	2090	0.0	20.49796	1
GAGTACT	29195	0.0	20.364319	12-13
CTACACT	10895	0.0	20.336536	4
CGTTAAC	550	0.0	17.975132	1
GTGATCG	1310	0.0	17.941784	8
TAGGCAT	2940	0.0	17.920277	5
CACTCTT	11515	0.0	17.839573	7
GTACTTT	33555	0.0	17.825798	14-15
TACTTTT	35440	0.0	17.772598	14-15
ACACTCT	12355	0.0	17.007156	6
ACTCTTT	13800	0.0	16.520742	8
ACTTTTT	37070	0.0	16.340797	16-17
ATACCGA	1025	0.0	16.051334	6
CATGGGG	14475	0.0	16.021814	4
>>END_MODULE