##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765554_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7021801 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.652126000152954 34.0 31.0 34.0 31.0 34.0 2 32.78367088443549 34.0 31.0 34.0 31.0 34.0 3 32.83333948085399 34.0 31.0 34.0 31.0 34.0 4 36.29438359190185 37.0 37.0 37.0 35.0 37.0 5 36.23419419035088 37.0 37.0 37.0 35.0 37.0 6 36.29286517803623 37.0 37.0 37.0 35.0 37.0 7 36.23914904452575 37.0 37.0 37.0 35.0 37.0 8 36.24139319812681 37.0 37.0 37.0 35.0 37.0 9 38.01054458820465 39.0 38.0 39.0 35.0 39.0 10-11 37.957441189233364 39.0 38.0 39.0 35.0 39.0 12-13 37.94359908519196 39.0 38.0 39.0 35.0 39.0 14-15 39.32654927133366 41.0 39.0 41.0 36.0 41.0 16-17 39.3211470960228 41.0 39.0 41.0 36.0 41.0 18-19 39.3584160246068 41.0 39.0 41.0 36.0 41.0 20-21 39.34919616776379 41.0 39.0 41.0 36.0 41.0 22-23 39.279204936169506 41.0 39.0 41.0 36.0 41.0 24-25 39.22299058318514 41.0 39.0 41.0 36.0 41.0 26-27 39.08957851411625 40.5 39.0 41.0 35.0 41.0 28-29 38.96590824775581 40.0 38.5 41.0 35.0 41.0 30-31 38.78355766561883 40.0 38.0 41.0 35.0 41.0 32-33 38.54248867206576 40.0 38.0 41.0 35.0 41.0 34-35 38.44115555254271 40.0 38.0 41.0 35.0 41.0 36-37 38.31250073877058 40.0 38.0 41.0 34.0 41.0 38-39 38.11811307383961 40.0 38.0 41.0 34.0 41.0 40-41 37.896487738686986 40.0 37.5 41.0 33.5 41.0 42-43 37.63213682643527 40.0 37.0 41.0 33.0 41.0 44-45 37.28815755388112 40.0 37.0 41.0 33.0 41.0 46-47 36.92391033297583 40.0 36.0 41.0 32.0 41.0 48-49 36.59816000197101 40.0 36.0 41.0 31.0 41.0 50-51 36.28486174700764 39.0 35.5 41.0 31.0 41.0 52-53 36.12076189570169 39.0 35.0 41.0 30.5 41.0 54-55 36.08279670699868 39.0 35.0 41.0 31.0 41.0 56-57 35.892660159409246 39.0 35.0 41.0 30.0 41.0 58-59 35.74622080574485 39.0 35.0 41.0 30.0 41.0 60-61 35.44888220272833 38.0 35.0 41.0 30.0 41.0 62-63 35.12271210762025 37.5 35.0 40.0 29.0 41.0 64-65 34.85929037293994 37.0 35.0 40.0 29.0 41.0 66-67 34.485800152980694 36.5 35.0 39.0 29.0 41.0 68-69 34.138665706988846 36.0 35.0 39.0 29.0 41.0 70-71 33.77717945866025 35.0 34.5 38.5 28.5 40.5 72-73 33.39322084177549 35.0 34.0 37.0 28.0 39.5 74-75 33.04447263600891 35.0 34.0 37.0 27.0 39.0 76-77 31.63856601746475 34.5 32.0 35.5 25.0 37.5 78-79 32.39363085054674 35.0 34.0 36.0 26.0 37.0 80-81 32.3272992925889 35.0 34.0 35.5 26.5 37.0 82-83 32.15245526040969 35.0 34.0 35.0 26.0 37.0 84-85 31.967797221823858 35.0 34.0 35.0 26.0 36.0 86-87 31.808858368387256 35.0 34.0 35.0 25.5 36.0 88-89 31.663843506815418 35.0 34.0 35.0 25.0 36.0 90-91 31.542139117870185 35.0 34.0 35.0 25.0 35.5 92-93 31.419316782119004 35.0 34.0 35.0 24.0 35.0 94-95 31.319743538730307 35.0 34.0 35.0 24.0 35.0 96-97 31.21163979155775 35.0 33.0 35.0 23.0 35.0 98-99 31.09282412019367 35.0 33.0 35.0 20.0 35.0 100 30.781608023354693 35.0 33.0 35.0 18.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3415730533680019 1101 2 0.32454890254056323 1101 3 0.3543488948808289 1101 4 0.23526781491160875 1101 5 0.2614223756858607 1101 6 0.22667501495153175 1101 7 0.25651985091288765 1101 8 0.2388087106336414 1101 9 0.32713044383769585 1101 10-11 0.27209032715375514 1101 12-13 0.3111397119277015 1101 14-15 0.4480269138254087 1101 16-17 0.4740681378021847 1101 18-19 0.41464261524147616 1101 20-21 0.46863077738268544 1101 22-23 0.48473692318254535 1101 24-25 0.4583045448685965 1101 26-27 0.4519860404841083 1101 28-29 0.4672400792902778 1101 30-31 0.47964840478057624 1101 32-33 0.4820448011486249 1101 34-35 0.48419344598769243 1101 36-37 0.5298975051610171 1101 38-39 0.5693766990871083 1101 40-41 0.6058959294010222 1101 42-43 0.6237600222265343 1101 44-45 0.572275908732081 1101 46-47 0.5728648276427251 1101 48-49 0.6830711860375303 1101 50-51 0.6351605895036414 1101 52-53 0.708445078498606 1101 54-55 0.7385672927612816 1101 56-57 0.7677010190963642 1101 58-59 0.789651248635316 1101 60-61 0.8243517434837102 1101 62-63 0.8377576985177768 1101 64-65 0.8627222158050927 1101 66-67 0.8485536479218183 1101 68-69 0.8235503854706181 1101 70-71 0.8032682444342001 1101 72-73 0.841748595260718 1101 74-75 0.8609457928981641 1101 76-77 0.8632783572786202 1101 78-79 0.8671396751350358 1101 80-81 0.8173741271579189 1101 82-83 0.8620895970832194 1101 84-85 0.8302257422272206 1101 86-87 0.8083107963279481 1101 88-89 0.8594433497990259 1101 90-91 0.8990520401697673 1101 92-93 0.9716189876724819 1101 94-95 0.9453046414094324 1101 96-97 1.0055567926967477 1101 98-99 0.9722546613976455 1101 100 0.9196351865913925 1102 1 0.3343668004743421 1102 2 0.2706933887043945 1102 3 0.29706909586246155 1102 4 0.20816001523687078 1102 5 0.253642336286795 1102 6 0.24626605010932678 1102 7 0.249207208369441 1102 8 0.25308256753331193 1102 9 0.30113430408343334 1102 10-11 0.3183049771599471 1102 12-13 0.3410309900666917 1102 14-15 0.45805013883079226 1102 16-17 0.44484581092030595 1102 18-19 0.3821182097290361 1102 20-21 0.4055233141257091 1102 22-23 0.38470800508002156 1102 24-25 0.373343521763573 1102 26-27 0.40946555051895217 1102 28-29 0.4341947693802197 1102 30-31 0.47989713008806234 1102 32-33 0.43442094912899876 1102 34-35 0.3888469891794415 1102 36-37 0.4513248151405307 1102 38-39 0.48058232391316125 1102 40-41 0.4692183938948702 1102 42-43 0.4608550515223584 1102 44-45 0.46486467800759357 1102 46-47 0.5064815375329275 1102 48-49 0.4845339285105581 1102 50-51 0.5236178721006581 1102 52-53 0.5236468493332609 1102 54-55 0.5696327413235167 1102 56-57 0.6719685047965527 1102 58-59 0.5753871024245996 1102 60-61 0.6263606875219949 1102 62-63 0.5848823552901834 1102 64-65 0.6493554030885633 1102 66-67 0.5825052449104575 1102 68-69 0.5799363567180933 1102 70-71 0.5579054193544906 1102 72-73 0.5557582555857579 1102 74-75 0.48289313495916275 1102 76-77 0.4996876967229973 1102 78-79 0.5990256769135698 1102 80-81 0.6455370014165069 1102 82-83 0.5951399598432872 1102 84-85 0.5760539726483671 1102 86-87 0.6044009602394418 1102 88-89 0.6163062146267286 1102 90-91 0.5805959870447204 1102 92-93 0.6437691267739858 1102 94-95 0.6152220791569221 1102 96-97 0.6558374069192219 1102 98-99 0.623893126193682 1102 100 0.6821281607433498 1103 1 0.358595150881591 1103 2 0.31658048935559435 1103 3 0.3102454143896267 1103 4 0.2110784018744596 1103 5 0.24163949732273693 1103 6 0.24405334846488813 1103 7 0.2693246701459131 1103 8 0.2694693377444608 1103 9 0.2979657960717361 1103 10-11 0.3303474959699102 1103 12-13 0.35739842342741923 1103 14-15 0.46459504957771003 1103 16-17 0.4495537051564753 1103 18-19 0.4464650538402779 1103 20-21 0.46231326993047617 1103 22-23 0.461843849032455 1103 24-25 0.45660134967435084 1103 26-27 0.4992402900460746 1103 28-29 0.48762470374906997 1103 30-31 0.5696526113698468 1103 32-33 0.5792324209686157 1103 34-35 0.5973545493691574 1103 36-37 0.6142572425324602 1103 38-39 0.6441002334053181 1103 40-41 0.586159867599946 1103 42-43 0.6451057905437665 1103 44-45 0.6239193594449333 1103 46-47 0.6848797036574794 1103 48-49 0.6248173073808587 1103 50-51 0.6562503352641897 1103 52-53 0.6098400740215837 1103 54-55 0.6907895226516274 1103 56-57 0.7101014969880168 1103 58-59 0.7559018518980949 1103 60-61 0.7260162431924897 1103 62-63 0.771094605552797 1103 64-65 0.7502363333979574 1103 66-67 0.7164683978030908 1103 68-69 0.6472597080652989 1103 70-71 0.6250987736154272 1103 72-73 0.6851177603033065 1103 74-75 0.675142365643346 1103 76-77 0.68682277066004 1103 78-79 0.7807912690728571 1103 80-81 0.7320878006274043 1103 82-83 0.7278449912194489 1103 84-85 0.7541805267691117 1103 86-87 0.7982702888473305 1103 88-89 0.8026817590887596 1103 90-91 0.8287762557917269 1103 92-93 0.8607007779878835 1103 94-95 0.8880915284707775 1103 96-97 0.945309350234016 1103 98-99 0.9458351943103942 1103 100 1.0063265877675995 1104 1 0.24628381575822544 1104 2 0.1948227938493048 1104 3 0.19590175932825815 1104 4 0.12386135973430612 1104 5 0.13805215590063824 1104 6 0.15219711278745507 1104 7 0.19678737223134135 1104 8 0.17823879827697198 1104 9 0.14242923262584384 1104 10-11 0.22047939574482456 1104 12-13 0.20779736294391427 1104 14-15 0.33794583693004654 1104 16-17 0.30273368803273826 1104 18-19 0.29787578962707784 1104 20-21 0.3100244910565664 1104 22-23 0.2773826833471489 1104 24-25 0.24490219493150533 1104 26-27 0.31524465828301373 1104 28-29 0.31682379136366734 1104 30-31 0.25547411648574325 1104 32-33 0.26859423847123054 1104 34-35 0.2316288891131819 1104 36-37 0.24960349576912932 1104 38-39 0.2951388707862961 1104 40-41 0.3216139071034618 1104 42-43 0.2689102789220641 1104 44-45 0.2792933751145057 1104 46-47 0.29013537436392767 1104 48-49 0.2868480435871632 1104 50-51 0.32187152275518827 1104 52-53 0.2909357370898178 1104 54-55 0.3344664839720082 1104 56-57 0.3496159115793631 1104 58-59 0.3231785801271414 1104 60-61 0.2439130806425922 1104 62-63 0.25630333973428066 1104 64-65 0.31153309257494044 1104 66-67 0.176041914911373 1104 68-69 0.25103848494907055 1104 70-71 0.22361255893233078 1104 72-73 0.2111189316945783 1104 74-75 0.2236763243393156 1104 76-77 0.15345052671031212 1104 78-79 0.2565169587110745 1104 80-81 0.2502198559827278 1104 82-83 0.27130779384449966 1104 84-85 0.24401310881413707 1104 86-87 0.25880576878683925 1104 88-89 0.23744471090285657 1104 90-91 0.2612315819172615 1104 92-93 0.2609631497355096 1104 94-95 0.27212409748871025 1104 96-97 0.3301193412654513 1104 98-99 0.33692979874532725 1104 100 0.3868258990450357 1105 1 0.22174565625267206 1105 2 0.2194190721687903 1105 3 0.22706084053427134 1105 4 0.15953069866000646 1105 5 0.19404944819793002 1105 6 0.15917406505851517 1105 7 0.21428528279393788 1105 8 0.17895704114656752 1105 9 0.24519642522110274 1105 10-11 0.24363500112875158 1105 12-13 0.21927981775897365 1105 14-15 0.3464660286344241 1105 16-17 0.3407757539547447 1105 18-19 0.369749279446026 1105 20-21 0.31032664284489186 1105 22-23 0.2508575076374697 1105 24-25 0.23121578223049966 1105 26-27 0.28189600359750244 1105 28-29 0.2765974619083522 1105 30-31 0.27891392286979055 1105 32-33 0.32733277067159605 1105 34-35 0.28284075752447535 1105 36-37 0.27578705173230844 1105 38-39 0.31885635301269843 1105 40-41 0.3706033120701022 1105 42-43 0.2984278074374629 1105 44-45 0.3211016194320564 1105 46-47 0.33236799169372233 1105 48-49 0.30717756880849123 1105 50-51 0.3352575536499387 1105 52-53 0.25816726667317624 1105 54-55 0.2828073120345991 1105 56-57 0.2762482927248655 1105 58-59 0.30760701765223786 1105 60-61 0.32869437597938855 1105 62-63 0.31611985705364276 1105 64-65 0.310928947504145 1105 66-67 0.3092019449862633 1105 68-69 0.3613613743227333 1105 70-71 0.32639421518697276 1105 72-73 0.31939086095997027 1105 74-75 0.2920898954944775 1105 76-77 0.24184027390694496 1105 78-79 0.3381740375786464 1105 80-81 0.3392621982068533 1105 82-83 0.33350324630620065 1105 84-85 0.3499629669130897 1105 86-87 0.34926978966142386 1105 88-89 0.33864874516220667 1105 90-91 0.3420354898051734 1105 92-93 0.3677356268103473 1105 94-95 0.37378929132776406 1105 96-97 0.36372494715263315 1105 98-99 0.48595837843431866 1105 100 0.4918786932509924 1106 1 0.18438266873171472 1106 2 0.17997783976123571 1106 3 0.14800435670271384 1106 4 0.11723904975017518 1106 5 0.13895772042720012 1106 6 0.1471836737896055 1106 7 0.16629729699528184 1106 8 0.164977432916821 1106 9 0.14551826491949527 1106 10-11 0.20359889151809085 1106 12-13 0.17733733091096582 1106 14-15 0.23183127349555122 1106 16-17 0.23046632256883015 1106 18-19 0.192596361570871 1106 20-21 0.22500468880979696 1106 22-23 0.23288525195893328 1106 24-25 0.22679369529893023 1106 26-27 0.2241464429477773 1106 28-29 0.3080905769773423 1106 30-31 0.2590440041996871 1106 32-33 0.2776901055115033 1106 34-35 0.2668641487357064 1106 36-37 0.3038728483528814 1106 38-39 0.3383556080353358 1106 40-41 0.4176887872425752 1106 42-43 0.3721296954338058 1106 44-45 0.35269236789559955 1106 46-47 0.35990718392351795 1106 48-49 0.4168582812600903 1106 50-51 0.4026268318652768 1106 52-53 0.40894231967053685 1106 54-55 0.4598758431357126 1106 56-57 0.4080333604090498 1106 58-59 0.4146143300284777 1106 60-61 0.35586029391387086 1106 62-63 0.4326172127332839 1106 64-65 0.41883526547012906 1106 66-67 0.3967742532838585 1106 68-69 0.3994226859673162 1106 70-71 0.42468383717048397 1106 72-73 0.47307235061109765 1106 74-75 0.44528139313660375 1106 76-77 0.3161185470175454 1106 78-79 0.40945048869878065 1106 80-81 0.3721804852679824 1106 82-83 0.3319765128614165 1106 84-85 0.4110007835490208 1106 86-87 0.3991145866475385 1106 88-89 0.3729226473714995 1106 90-91 0.3878490718552534 1106 92-93 0.4323583101202466 1106 94-95 0.4284654788981719 1106 96-97 0.44038741857268704 1106 98-99 0.4395646040752226 1106 100 0.438290070144852 1107 1 0.08820480025072897 1107 2 0.13233905524796086 1107 3 0.09614033167933655 1107 4 0.06363425754689445 1107 5 0.04842554700177004 1107 6 0.07972086716431903 1107 7 0.06465672443207637 1107 8 0.06681951515779616 1107 9 0.07605120741793314 1107 10-11 0.09671109873253414 1107 12-13 0.14278222172051613 1107 14-15 0.16297333436740757 1107 16-17 0.1519423995578819 1107 18-19 0.1430927318689399 1107 20-21 0.14752950172248092 1107 22-23 0.14851608275139938 1107 24-25 0.15641567171258686 1107 26-27 0.15316219173447365 1107 28-29 0.14807581669148817 1107 30-31 0.16679781980573694 1107 32-33 0.14559435377042718 1107 34-35 0.15591678434353895 1107 36-37 0.111763541924077 1107 38-39 0.1675889291901811 1107 40-41 0.20552222700317913 1107 42-43 0.1134048624069095 1107 44-45 0.08355941345770646 1107 46-47 0.09807110098983429 1107 48-49 0.17032363640330317 1107 50-51 0.1526287142118008 1107 52-53 0.13064742445090616 1107 54-55 0.18008271212296023 1107 56-57 0.14790472263993593 1107 58-59 0.1156853986491555 1107 60-61 0.1656707120386116 1107 62-63 0.11091623566994713 1107 64-65 0.1550228979514685 1107 66-67 0.11348143671261113 1107 68-69 0.1275973261343708 1107 70-71 0.14868614421023807 1107 72-73 0.13840314881207405 1107 74-75 0.13220620181849085 1107 76-77 0.11614493124140424 1107 78-79 0.13197645342827258 1107 80-81 0.21502144634751374 1107 82-83 0.2525259151357204 1107 84-85 0.1793122729978407 1107 86-87 0.21787072220086756 1107 88-89 0.21680857968159728 1107 90-91 0.1985593188477992 1107 92-93 0.21977225551140123 1107 94-95 0.2592465146692078 1107 96-97 0.2988294469606245 1107 98-99 0.2743829370189381 1107 100 0.3208006776157255 1108 1 0.15241518692571532 1108 2 0.14342365710963634 1108 3 0.11985511128350623 1108 4 0.06213195188354348 1108 5 0.12805561638939622 1108 6 0.09566352667889078 1108 7 0.1309767610026924 1108 8 0.13470105594378623 1108 9 0.1191835129327572 1108 10-11 0.15390580298112155 1108 12-13 0.13290978721614266 1108 14-15 0.20699937767964371 1108 16-17 0.20517985149017903 1108 18-19 0.21872773233139498 1108 20-21 0.21013762452331264 1108 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-0.7836116164295888 2316 54-55 -0.8206109308249907 2316 56-57 -0.8680778144937449 2316 58-59 -0.8670623892205143 2316 60-61 -0.8398841814111435 2316 62-63 -0.8043500772231411 2316 64-65 -0.7491148102982308 2316 66-67 -0.7606628786560492 2316 68-69 -0.719577503251422 2316 70-71 -0.7129840978277642 2316 72-73 -0.7299667327615893 2316 74-75 -0.6786817447892943 2316 76-77 -0.7528297317118948 2316 78-79 -0.7679899882618102 2316 80-81 -0.6824291675291079 2316 82-83 -0.7680246293243336 2316 84-85 -0.7398785849240106 2316 86-87 -0.7343013681577553 2316 88-89 -0.7425576387045929 2316 90-91 -0.8203807403653691 2316 92-93 -0.7962223040697545 2316 94-95 -0.7733876349360358 2316 96-97 -0.819446345893649 2316 98-99 -0.8222738816950965 2316 100 -0.8604696037304542 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15.0 3 19.0 4 35.0 5 110.0 6 203.0 7 585.0 8 1452.0 9 2580.0 10 4892.0 11 9053.0 12 15307.0 13 22141.0 14 25720.0 15 34095.0 16 46864.0 17 64304.0 18 66112.0 19 41192.0 20 26827.0 21 22127.0 22 22417.0 23 24430.0 24 26988.0 25 29569.0 26 34896.0 27 43234.0 28 53668.0 29 68185.0 30 86093.0 31 108945.0 32 139628.0 33 184068.0 34 268408.0 35 452273.0 36 914783.0 37 1893904.0 38 1895667.0 39 390516.0 40 496.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.9666942505423 18.66771304983186 13.928480375330615 23.437112324295224 2 15.568489267613714 22.35071396075784 36.53109065824818 25.549706113380267 3 18.979851090615973 26.956215855944127 29.90556444859664 24.158368604843258 4 13.678143256979228 18.523367438068952 34.886448647576316 32.912040657375506 5 13.964886266097782 37.26282976892908 32.758249181074035 16.014034783899106 6 31.246485054190515 34.92670897395127 17.755387827140073 16.07141814471814 7 26.14683896624242 32.840677199482016 21.76820448201252 19.244279352263042 8 27.938501817411233 31.2626062743732 20.92631220964536 19.87257969857021 9 28.145627504816122 14.576165992307017 19.578285842378005 37.699920660498854 10-11 25.217825170494006 25.820099430331332 25.96318238013296 22.998893019041695 12-13 27.221363863772268 24.028037821066135 26.384726653461126 22.365871661700467 14-15 25.401261015514397 23.672708753779837 25.24483391084424 25.681196319861527 16-17 23.178362645139046 26.729175321260172 26.761837881762812 23.33062415183797 18-19 24.307125764458434 25.619011418865327 28.575396824831696 21.498465991844544 20-21 24.95982846473172 25.170202981786765 27.647971029497864 22.22199752398365 22-23 23.762836833132315 25.866127081390246 26.15916199079737 24.21187409468007 24-25 23.605551063842817 26.565194152364434 25.888287425054184 23.94096735873856 26-27 24.157734328166466 25.61464112102599 26.36177007071857 23.865854480088977 28-29 23.58009682034402 24.935671360280757 28.265457761781338 23.218774057593887 30-31 24.779282286810687 25.02696751023868 27.3831744953864 22.810575707564233 32-33 22.64181495917575 25.961830622220063 27.63263367795211 23.763720740652072 34-35 22.655707572140702 26.403971981018554 27.777924947264665 23.16239549957608 36-37 23.91227692154762 26.30715396235239 26.744021939670464 23.036547176429522 38-39 24.175373538453563 25.205194878436092 26.766895470755685 23.85253611235466 40-41 23.38462016104896 25.960729905000296 26.874823714955458 23.779826218995286 42-43 25.010387523891303 25.300704129922842 26.68678256211917 23.00212578406668 44-45 24.532098770267236 25.282585034352696 27.040719432298477 23.144596763081598 46-47 23.588881639877368 25.56747013912833 27.78901445771911 23.0546337632752 48-49 24.182102571960957 26.228316578663584 27.04911927612079 22.540461573254657 50-51 23.176290013112954 26.84285425893296 25.907337735649538 24.073517992304552 52-53 22.935085011250045 26.88516210516961 24.99374760194186 25.186005281638487 54-55 23.60518334366341 26.213966737913815 24.970113513107044 25.210736405315735 56-57 24.037861985485605 25.130387499211142 25.360292025830905 25.471458489472344 58-59 23.618938497656462 25.11995451086791 26.68422030208909 24.576886689386544 60-61 23.748160601980015 25.62896355062914 25.963735592762106 24.659140254628735 62-63 22.217394478163875 25.98645802361211 26.42203749236639 25.374110005857624 64-65 22.262000045573398 25.904845591628167 26.1131729909252 25.719981371873235 66-67 23.530201335591197 25.885084982100803 25.347436217050195 25.2372774652578 68-69 23.706099311707874 25.139827028694423 25.39152173292306 25.762551926674647 70-71 22.935960446614764 25.52038999681136 25.608195105500712 25.935454451073163 72-73 23.901620855725746 25.102801977403967 25.60859618004678 25.386980986823502 74-75 23.999429483416886 24.743294240509766 25.76702686163257 25.49024941444078 76-77 22.796221250272637 25.45651608742875 26.318738210277065 25.428524452021545 78-79 22.893166665396773 25.292652878377275 26.270174126602303 25.544006329623652 80-81 22.353530695520558 25.344006188835827 25.983483145619363 26.31897997002425 82-83 22.41857643296114 25.640029095549256 25.103755255588307 26.837639215901294 84-85 22.699297687580138 25.19585149595123 24.99303213449967 27.11181868196896 86-87 22.7828587757426 24.366872619583962 25.55593509054473 27.294333514128706 88-89 22.780959604781412 23.947266875442246 26.192353185669436 27.079420334106906 90-91 23.134119997399512 24.22723617531529 25.711479336271864 26.92716449101333 92-93 22.21739674087037 24.50912535788789 25.978687370331503 27.294790530910234 94-95 21.72623667607266 24.630563023604033 26.418937913909406 27.2242623864139 96-97 22.58263022489264 24.28867547400629 25.75460405779434 27.374090243306732 98-99 22.643685003320375 23.96228545924329 25.715368464586223 27.678661072850115 100 22.7062447474556 24.313542916234013 25.844804251796276 27.135408084514108 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1255.0 1 809.5 2 389.0 3 430.5 4 379.0 5 466.0 6 812.0 7 1542.5 8 2513.5 9 2744.5 10 2080.5 11 1753.5 12 2009.5 13 2268.0 14 2588.0 15 3031.0 16 3532.0 17 4042.5 18 4625.5 19 5317.5 20 6306.5 21 7449.0 22 8755.0 23 10464.5 24 12610.0 25 15342.0 26 18992.0 27 23481.5 28 28334.5 29 35600.0 30 43977.5 31 51771.5 32 60833.0 33 70813.0 34 81605.5 35 91980.0 36 101620.5 37 112386.5 38 120677.0 39 128271.0 40 134583.5 41 139086.0 42 146177.5 43 160851.0 44 181079.0 45 200312.0 46 226571.0 47 260019.5 48 344028.5 49 425188.0 50 486901.5 51 476451.0 52 389449.0 53 342034.5 54 302308.5 55 264989.5 56 235005.0 57 205845.5 58 187827.5 59 170735.0 60 144032.0 61 117146.5 62 94198.5 63 72811.5 64 53686.5 65 41671.5 66 31659.0 67 22874.0 68 17056.0 69 13562.0 70 11351.0 71 9580.5 72 8493.0 73 8575.5 74 6419.5 75 4780.5 76 3719.0 77 2424.0 78 1724.0 79 1010.5 80 639.0 81 507.5 82 414.5 83 304.5 84 201.0 85 116.0 86 73.0 87 36.0 88 19.0 89 12.0 90 10.0 91 6.5 92 2.0 93 1.5 94 3.0 95 2.5 96 1.0 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011264916223060152 2 0.04668317999897747 3 0.0011535502074182962 4 0.0 5 0.0014811015008827507 6 0.0 7 0.0 8 0.0 9 0.001110826125662063 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 5.411717022456205E-4 22-23 0.0015950323855660393 24-25 0.0032470302134737223 26-27 0.008402402745392527 28-29 0.0034108058602059497 30-31 0.005539889267724904 32-33 0.002527841503910464 34-35 0.0059528887247018245 36-37 0.0 38-39 7.120680292705532E-6 40-41 0.0021006006863481318 42-43 0.0022287729316168314 44-45 0.0021860488498605985 46-47 0.0021931695301533037 48-49 0.0010965847650766518 50-51 0.0016876012293712112 52-53 0.007049473489778478 54-55 0.003318237016400778 56-57 0.03059044253746297 58-59 0.017417183995957732 60-61 0.021383402918994713 62-63 0.014789652967949391 64-65 0.0022928590542511816 66-67 0.001659118508200389 68-69 7.120680292705532E-6 70-71 0.0 72-73 0.0024281519798125865 74-75 0.0013458085753213457 76-77 8.616023154173695E-4 78-79 0.0030547718455706738 80-81 0.0017801700731763831 82-83 0.0015451876235171005 84-85 0.00401606368508592 86-87 7.191887095632588E-4 88-89 9.470504789298357E-4 90-91 5.767751037091481E-4 92-93 6.978266686851421E-4 94-95 4.984476204893873E-4 96-97 8.544816351246638E-5 98-99 0.0 100 0.0029052375594238572 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7021801.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.127488155767765 #Duplication Level Percentage of deduplicated Percentage of total 1 79.01675254209721 33.28777306824433 2 11.904433577442049 10.03007769069628 3 3.597479485234698 4.546583232145268 4 1.62694667663724 2.7415670740040423 5 0.879719227071281 1.8530180659423283 6 0.5578429387759124 1.410031307763656 7 0.36920615717999145 1.0887609609545634 8 0.26257701242164233 0.8849367984615698 9 0.21219344765035192 0.8045259257359556 >10 1.3028734391400933 10.518656653439393 >50 0.13409810814393217 3.9242569724261758 >100 0.1127330577591694 9.86490987269663 >500 0.013974602100474064 4.059847428387968 >1k 0.007782426271611769 5.835873728827433 >5k 7.105693551958581E-4 2.102228809498337 >10k+ 6.767327192341506E-4 7.046952410776217 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 63547 0.904995741121117 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 56951 0.8110597266997456 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 36260 0.5163917348270052 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 34911 0.49718013939728567 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 31734 0.4519353368174347 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 29445 0.4193368624374288 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 25468 0.362698971389249 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 23779 0.3386453133604897 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22739 0.32383429835166216 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 21501 0.3062034939469233 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 20219 0.28794606967642633 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 18583 0.2646472037586938 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 18510 0.26360758443595883 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16142 0.2298840425697054 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 14708 0.20946193149022596 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 13418 0.19109057633504567 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12965 0.18463923998985446 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 11644 0.16582640265652643 No Hit CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC 11613 0.1653849204783787 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 10229 0.14567487742816979 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 9582 0.13646071712940883 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC 9286 0.13224527439612715 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 8838 0.125865144853863 No Hit GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG 8459 0.1204676691919922 No Hit GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA 8239 0.11733456986320176 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 7967 0.11346091978396994 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 7864 0.11199405964367262 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 7645 0.10887520167546759 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 7479 0.10651113581828936 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 7440 0.10595572275545832 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 7426 0.10575634370726256 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0038594087186463984 0.0 0.0 0.0 0.0 2 0.00600985416704347 0.0 0.0 1.4241360585411064E-5 0.0 3 0.0062946813787516905 0.0 0.0 2.848272117082213E-5 0.0 4 0.007277335259145054 0.0 0.0 8.544816351246638E-5 0.0 5 0.030875269749171187 0.0 0.0 2.5634449053739915E-4 0.0 6 0.06414308807669143 0.0 0.0 4.842062599039762E-4 0.0 7 0.07227490497096116 0.0 0.0 4.984476204893873E-4 0.0 8 0.08298440813119028 0.0 0.0 5.411717022456205E-4 0.0 9 0.09084563917433718 0.0 0.0 6.6934394751432E-4 0.0 10-11 0.16467285244910815 0.0 0.0 0.0012105156497599404 0.0 12-13 0.28876494791008744 0.0 0.0 0.002848272117082213 1.4241360585411064E-5 14-15 0.43508068656460075 1.4241360585411064E-5 0.0 0.004628442190258595 1.4241360585411064E-5 16-17 0.4992095332807067 2.1362040878116596E-5 0.0 0.005461561784505143 1.4241360585411064E-5 18-19 0.5294937865655833 4.272408175623319E-5 0.0 0.005924406003531003 2.848272117082213E-5 20-21 0.640448226886521 4.272408175623319E-5 0.0 0.0076048865526095084 2.848272117082213E-5 22-23 0.6720711680664262 4.272408175623319E-5 0.0 0.010559968874082306 2.848272117082213E-5 24-25 0.8189565611443559 4.272408175623319E-5 0.0 0.022658004691389004 2.848272117082213E-5 26-27 1.1366101090019498 4.272408175623319E-5 0.0 0.05866728493160088 2.848272117082213E-5 28-29 1.2085289799582757 4.272408175623319E-5 0.0 0.10514396520208989 2.848272117082213E-5 30-31 1.2282888677705335 4.272408175623319E-5 0.0 0.2251203074538854 2.848272117082213E-5 32-33 1.2460976891825901 4.272408175623319E-5 0.0 0.399527129863122 2.848272117082213E-5 34-35 1.2603746531694648 4.272408175623319E-5 0.0 0.6196629611121136 2.848272117082213E-5 36-37 1.283239157589342 4.272408175623319E-5 0.0 0.9543776589510298 2.848272117082213E-5 38-39 1.305441438741998 4.272408175623319E-5 0.0 1.4106708521076003 2.848272117082213E-5 40-41 1.324546223967327 4.272408175623319E-5 0.0 2.0057247421281237 2.848272117082213E-5 42-43 1.362364157001886 4.272408175623319E-5 0.0 2.4556520471030154 2.848272117082213E-5 44-45 1.4055866863786086 4.272408175623319E-5 0.0 2.914188254551788 2.848272117082213E-5 46-47 1.4232958182665674 4.272408175623319E-5 0.0 3.441923233085073 3.560340146352766E-5 48-49 1.4352158370765564 4.272408175623319E-5 0.0 3.9210168445388867 4.272408175623319E-5 50-51 1.456065188973598 4.272408175623319E-5 0.0 4.4260510942990265 4.272408175623319E-5 52-53 1.4624097151143987 4.984476204893873E-5 0.0 4.995620069551957 4.272408175623319E-5 54-55 1.4829030329968051 6.408612263434979E-5 0.0 5.494786878750908 4.272408175623319E-5 56-57 1.523028066446201 7.832748321976086E-5 0.0 6.016818192369735 4.272408175623319E-5 58-59 1.5348127923306287 9.968952409787746E-5 0.0 6.544681343148289 4.272408175623319E-5 60-61 1.5421826964335787 1.2105156497599405E-4 0.0 7.024088264534981 4.272408175623319E-5 62-63 1.5483064814853056 1.2817224526869957E-4 0.0 7.51495948119293 4.272408175623319E-5 64-65 1.5536541123851273 1.4241360585411065E-4 0.0 8.02873080567222 4.272408175623319E-5 66-67 1.5608531201610527 1.4241360585411065E-4 0.0 8.545564022677372 4.272408175623319E-5 68-69 1.5688354027691755 1.4241360585411065E-4 0.0 9.139514207252526 4.272408175623319E-5 70-71 1.5795662679702829 1.5665496643952172E-4 0.0 9.813401148793593 4.272408175623319E-5 72-73 1.6046809073626553 1.7089632702493277E-4 0.0 10.375991857359672 4.272408175623319E-5 74-75 1.6282788418526812 1.922583679030494E-4 0.0 10.91274161714352 4.272408175623319E-5 76-77 1.6352642292198256 1.993790481957549E-4 0.0 11.483741849135287 4.272408175623319E-5 78-79 1.64035551562911 1.993790481957549E-4 0.0 12.046496333348097 4.272408175623319E-5 80-81 1.6516560352536336 1.993790481957549E-4 0.0 12.662015058529857 4.272408175623319E-5 82-83 1.6561847879197944 2.1362040878116596E-4 0.0 13.343912765400216 4.272408175623319E-5 84-85 1.6722917667418944 2.1362040878116596E-4 0.0 13.977275915395495 4.272408175623319E-5 86-87 1.6980187846394394 2.1362040878116596E-4 0.0 14.60010045855757 4.272408175623319E-5 88 1.7047763102372169 2.1362040878116596E-4 0.0 15.085787819962428 4.272408175623319E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCGATC 2235 0.0 28.387238 3 CGATCTC 2460 0.0 24.453018 5 TCTCGCG 1025 0.0 23.841354 8 CCGATCT 3060 0.0 21.194115 4 GATCTAC 3145 0.0 21.082352 1 TATCACG 300 1.9281288E-10 20.3745 2 AGTACTT 25900 0.0 20.321716 12-13 TCTACAC 4425 0.0 20.073145 3 CTCGCGC 1255 0.0 19.472021 9 TACACTC 4145 0.0 18.707556 5 GAGTACT 25125 0.0 18.498323 12-13 ATCTACA 3910 0.0 18.157877 2 GTATATA 1560 0.0 17.483322 1 TACTTTT 30350 0.0 17.458284 14-15 AGTACTC 2310 0.0 17.292768 5 GTATAAG 2035 0.0 17.099678 1 CTACACT 5790 0.0 17.045053 4 CATGGGG 17835 0.0 16.468897 4 CGCTATA 200 2.986276E-4 16.456327 2 GTACTTT 28505 0.0 16.370876 14-15 >>END_MODULE