FastQCFastQC Report
Fri 27 May 2016
ERR765553_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765553_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14162710
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA200000.14121591136159675No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT196640.13884348405072194No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG53000.021.094391
GTACTAG45850.020.3882371
GTATCAA500500.018.7148761
CATGGGG295200.018.4258464
GTACATG737850.017.9597761
TACATGG732950.017.6368712
TAGTACT56300.017.3536224
ACATGGG692850.016.9626643
CTAGTAC61250.016.7951553
GTATAAA124250.016.7105881
TGATCGC37500.016.657029
ATACGGG37500.016.4091453
TAGGCAT71500.015.962535
GTGATCG40100.015.9283768
AACGCAG558800.015.784196
CAACGCA557050.015.6658155
TATAAGG70050.015.5570542
TACGGGC39650.015.5189854
ATCAACG558250.015.4991143
CTAACGC22500.015.4488153
Produced by FastQC (version 0.11.5)