##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765553_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14162710
Sequences flagged as poor quality	0
Sequence length	100
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.91813311153021	33.0	33.0	33.0	27.0	33.0
2	31.843554446853744	33.0	33.0	33.0	27.0	33.0
3	31.8481589328596	33.0	33.0	33.0	27.0	33.0
4	35.6265773993819	37.0	37.0	37.0	33.0	37.0
5	35.66701485803211	37.0	37.0	37.0	33.0	37.0
6	35.67438145665625	37.0	37.0	37.0	33.0	37.0
7	35.48961837106034	37.0	37.0	37.0	33.0	37.0
8	35.484741903209205	37.0	37.0	37.0	33.0	37.0
9	35.376984489550374	37.0	37.0	37.0	33.0	37.0
10-11	35.33276978770306	37.0	37.0	37.0	33.0	37.0
12-13	35.286133762535556	37.0	37.0	37.0	33.0	37.0
14-15	36.925561739243406	40.0	37.0	40.0	33.0	40.0
16-17	36.95102642785173	40.0	37.0	40.0	33.0	40.0
18-19	36.88141549180912	40.0	37.0	40.0	33.0	40.0
20-21	36.57952704673046	40.0	37.0	40.0	33.0	40.0
22-23	36.61524443415138	40.0	37.0	40.0	33.0	40.0
24-25	36.495561195562146	38.5	37.0	40.0	33.0	40.0
26-27	36.46295860749814	37.0	37.0	40.0	33.0	40.0
28-29	36.394786026120705	37.0	37.0	40.0	33.0	40.0
30-31	36.5236003914505	37.0	37.0	40.0	33.0	40.0
32-33	36.31073145605608	37.0	37.0	40.0	33.0	40.0
34-35	36.15129558537879	37.0	37.0	40.0	27.0	40.0
36-37	36.2654656841805	37.0	37.0	40.0	33.0	40.0
38-39	36.20108496184699	37.0	37.0	40.0	33.0	40.0
40-41	36.2560465475887	37.0	37.0	40.0	33.0	40.0
42-43	36.19401068015938	37.0	37.0	40.0	33.0	40.0
44-45	36.11416748630735	37.0	37.0	40.0	30.0	40.0
46-47	35.946856675029	37.0	37.0	40.0	27.0	40.0
48-49	35.777728626795295	37.0	37.0	40.0	27.0	40.0
50-51	35.58357514204556	37.0	35.0	40.0	27.0	40.0
52-53	35.43197018790895	37.0	33.0	40.0	27.0	40.0
54-55	35.29226659304611	37.0	33.0	40.0	27.0	40.0
56-57	35.14085842328198	37.0	33.0	40.0	27.0	40.0
58-59	34.960379546004965	37.0	33.0	40.0	27.0	40.0
60-61	34.74060610575236	37.0	33.0	38.5	27.0	40.0
62-63	34.5170091387877	37.0	33.0	37.0	27.0	40.0
64-65	34.31746226534328	37.0	33.0	37.0	27.0	40.0
66-67	34.06415354123611	37.0	33.0	37.0	27.0	40.0
68-69	33.84271650693971	37.0	33.0	37.0	27.0	40.0
70-71	33.55239558672034	37.0	33.0	37.0	27.0	38.5
72-73	33.34454062817074	37.0	33.0	37.0	27.0	37.0
74-75	33.14925889889717	37.0	33.0	37.0	27.0	37.0
76-77	32.903184383497226	37.0	33.0	37.0	27.0	37.0
78-79	32.809615920964276	37.0	33.0	37.0	27.0	37.0
80-81	32.65141311232102	33.0	33.0	37.0	27.0	37.0
82-83	32.51033117955532	33.0	33.0	37.0	22.0	37.0
84-85	32.36654662137401	33.0	33.0	37.0	22.0	37.0
86-87	32.21493107604407	33.0	33.0	37.0	22.0	37.0
88-89	32.09254355275226	33.0	33.0	37.0	22.0	37.0
90-91	31.983946822324256	33.0	33.0	37.0	22.0	37.0
92-93	31.881671975208135	33.0	33.0	37.0	22.0	37.0
94-95	31.750661773064618	33.0	33.0	37.0	22.0	37.0
96-97	31.622160200978485	33.0	33.0	37.0	22.0	37.0
98-99	31.51484984159105	33.0	33.0	37.0	22.0	37.0
100	31.30122292979239	33.0	33.0	37.0	22.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	17.0
7	435.0
8	2386.0
9	4503.0
10	6373.0
11	9360.0
12	14543.0
13	22217.0
14	30410.0
15	35429.0
16	41040.0
17	46619.0
18	53637.0
19	62638.0
20	71453.0
21	84034.0
22	97822.0
23	106458.0
24	113556.0
25	127995.0
26	149243.0
27	179171.0
28	220426.0
29	275234.0
30	343287.0
31	433402.0
32	566888.0
33	761920.0
34	1032848.0
35	1478211.0
36	2441594.0
37	3858471.0
38	1490079.0
39	1010.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.388538425207166	18.18503267730148	12.757805490961765	25.668623406529584
2	16.165079773954904	20.561323710084338	37.224886991222185	26.048709524738573
3	18.178797172489336	25.178456528972493	29.292073005046383	27.350673293491788
4	13.577731665358664	17.05046475923569	35.938499848109586	33.43330372729606
5	14.719888970990832	36.643167682716474	33.435857576143	15.201085770149698
6	32.43431850515163	36.1611161370336	17.198067353135812	14.206498004678956
7	28.069146794134127	31.370038376120448	21.955232897385088	18.60558193236034
8	27.130864581809988	32.76129245193153	20.01467074466669	20.093172221591797
9	27.104203043359853	14.549010910519	19.210720991266346	39.136065054854804
10-11	25.648511181439087	24.65210792060364	27.186089901921523	22.513290996035746
12-13	26.545169504950035	22.56018244697709	26.681038106014647	24.213609942058227
14-15	24.32330327235867	23.52901386667574	24.160533696391333	27.987149164574248
16-17	24.29006260818764	26.085293845492895	24.936038424288416	24.688605122031053
18-19	24.602396187841578	25.48688441670129	26.37575044375516	23.534968951701977
20-21	25.545658363348668	24.90284339089682	25.67835921222314	23.87313903353138
22-23	25.143655230835588	24.591543330168772	25.48806622814736	24.776735210848283
24-25	24.74234179517149	25.047596515450593	25.570932191120544	24.639129498257372
26-27	24.848170047428088	25.065741687036198	25.43715877965942	24.648929485876295
28-29	24.5375039491168	24.946389834491306	25.913145634245637	24.602960582146256
30-31	24.935287003762845	24.916163402222562	25.671582744839665	24.47696684917493
32-33	24.567959371123166	24.969500937009194	25.42626289994527	25.03627679192237
34-35	24.8716861784298	25.003176985163638	25.74944885753212	24.375687978874446
36-37	24.9862575982308	24.87143737681814	25.335440336785265	24.806864688165795
38-39	24.93942372263198	24.71144083258046	25.48816075188438	24.860974692903184
40-41	24.814660393348557	24.81437784327053	25.678264111213124	24.692697652167787
42-43	25.073663969663397	24.790303786389146	25.417991944664514	24.718040299282944
44-45	24.76985556442921	24.996422204456074	25.239535707388455	24.99418652372626
46-47	24.58838335844623	24.923666186047992	25.575413506245386	24.912536949260396
48-49	25.08751204696847	25.040825297959156	25.16123670365415	24.71042595141823
50-51	24.435804218310846	25.322199161133014	25.08102022202153	25.16097639853462
52-53	24.526763713755493	25.090761804437907	24.990348170757635	25.39212631104897
54-55	24.634587756395273	24.97836099559126	25.11528265678028	25.271768591233183
56-57	24.715403092556638	24.768147943498143	24.93350130327841	25.58294766066681
58-59	24.432018253496647	24.801535315230595	25.35410006625847	25.412346365014283
60-61	24.539092841758357	24.874316345575448	25.22455080569569	25.36204000697051
62-63	24.245281596408326	24.90987144407778	25.10575572372608	25.739091235787814
64-65	24.19912819132895	24.756901406660184	25.2398286045623	25.80414179744856
66-67	24.668025246014736	24.725621495669017	25.094548251884962	25.51180500643129
68-69	24.561046713964522	24.51986358503481	25.138560464245668	25.780529236755
70-71	24.149131043226983	24.646069251263285	25.182347223636743	26.02245248187299
72-73	24.55352131193205	24.68685408495957	25.111342241183472	25.648282361924903
74-75	24.699163206880083	24.638992493668216	25.110453337241132	25.55139096221057
76-77	24.383826472968963	24.815352934616534	25.130628568789167	25.670192023625333
78-79	24.44993193760097	24.859747104787843	25.125986417465672	25.564334540145516
80-81	24.168258587926264	24.788294806643584	25.343684255670684	25.69976234975947
82-83	24.318671036671795	24.782413182450163	25.098273557932917	25.800642222945125
84-85	24.25182371170587	24.878184971256665	24.9182838160568	25.951707500980664
86-87	24.141666231119007	24.62302997085833	25.16753634939492	26.067767448627745
88-89	24.2110328027537	24.311414801727455	25.26846048798541	26.209091907533434
90-91	24.36610554432121	24.504330235140536	25.053068944106883	26.076495276431373
92-93	24.250320162143677	24.494248347081154	25.08148273557667	26.173948755198495
94-95	23.828581843400414	24.502133312040883	25.43887866859942	26.23040617595928
96-97	24.224381776750093	24.469882336945243	25.122714081513912	26.18302180479075
98-99	23.98377812502748	24.50193688415872	25.102957394269687	26.41132759654411
100	23.968463438520192	24.529288289148674	25.06907708660977	26.433171185721367
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	727.0
1	795.0
2	1105.0
3	1408.0
4	1246.0
5	1086.0
6	1393.0
7	2634.5
8	4391.5
9	4416.0
10	2930.0
11	2021.0
12	1908.5
13	2011.5
14	2262.5
15	2689.0
16	3088.5
17	3644.0
18	4282.0
19	5091.5
20	6332.5
21	7886.0
22	10177.5
23	13400.0
24	17305.5
25	22033.0
26	28851.5
27	37086.5
28	45366.0
29	56873.5
30	70438.5
31	83231.5
32	97682.0
33	113275.0
34	130138.5
35	146368.5
36	163233.5
37	185768.5
38	210333.0
39	237981.5
40	260961.5
41	282876.5
42	309380.0
43	347524.5
44	397882.5
45	450246.0
46	509353.0
47	563706.0
48	615505.5
49	653801.0
50	682018.5
51	687564.5
52	666822.0
53	661096.0
54	649509.0
55	616723.0
56	581853.0
57	534683.0
58	492431.0
59	453061.5
60	393665.0
61	329153.0
62	270421.0
63	214740.5
64	163933.5
65	128086.0
66	99974.5
67	75614.0
68	58999.0
69	48308.5
70	41568.0
71	35872.5
72	30568.0
73	29031.5
74	24333.0
75	18998.5
76	14606.0
77	10344.5
78	7625.5
79	5238.5
80	3688.5
81	2693.0
82	1929.5
83	1302.5
84	860.0
85	586.5
86	364.5
87	221.5
88	152.5
89	92.5
90	52.0
91	42.0
92	32.5
93	18.5
94	17.5
95	15.5
96	10.5
97	6.0
98	4.0
99	4.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.017101246865889368
2	0.005345022245036438
3	0.009023696736006032
4	0.008359981952606527
5	0.019819653159600104
6	0.004081139838350146
7	0.02389373220238217
8	0.008423529112719248
9	9.814505839630975E-4
10-11	1.4474630914563667E-4
12-13	9.143730260663391E-4
14-15	3.601005739720717E-4
16-17	7.44913932432423E-4
18-19	0.002397140095363105
20-21	0.005514481338670353
22-23	0.01215868996823348
24-25	0.021606034438324304
26-27	0.026093170021839043
28-29	0.03163942494056576
30-31	0.03397654827360018
32-33	0.03458377669245504
34-35	0.04382635809107155
36-37	0.04092437111259074
38-39	0.04507964930440572
40-41	0.041852865729793236
42-43	0.04854296953054889
44-45	0.04199761203893888
46-47	0.04450772486339125
48-49	0.04701783768784364
50-51	0.04822876412776933
52-53	0.03369411645087699
54-55	0.030001320368771234
56-57	0.021422453753554227
58-59	0.02126711625105647
60-61	0.021553078471563705
62-63	0.02170841597406146
64-65	0.025701295867810614
66-67	0.026845144749839546
68-69	0.028239651874535313
70-71	0.028299668636863992
72-73	0.02665803366728543
74-75	0.021164734715319312
76-77	0.025920180530421083
78-79	0.02572953905008293
80-81	0.024405639881067958
82-83	0.026576834518252507
84-85	0.0278760209027792
86-87	0.030290812987062505
88-89	0.0303261169649029
90-91	0.029944834004226595
92-93	0.032829168993787204
94-95	0.03054853202529742
96-97	0.03316808718105504
98-99	0.033824741168886466
100	0.042802542733699975
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.416271E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.1534649179687
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.10803218579795	28.15080410840259
2	14.772145975240159	11.863056903598364
3	5.8501504575072065	7.047114334810624
4	2.8697140125324956	4.609158437073069
5	1.6662025133467289	3.3451902082949583
6	1.0045563276109983	2.420185035531101
7	0.7051814774262035	1.982083580024408
8	0.4678335753145602	1.5028111243072848
9	0.355513468230395	1.284758781700909
>10	1.9370326218457161	14.141153010697675
>50	0.14267345740253495	3.984974089711186
>100	0.0972711651301134	7.766282520315536
>500	0.013995771759572662	3.9043979231720884
>1k	0.009283212205267536	6.740286753171401
>5k	3.5980752206011207E-4	0.8751801173944895
>10k+	5.397112830901681E-5	0.38256307179429955
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	20000	0.14121591136159675	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	19664	0.13884348405072194	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	7.060795568079838E-6	2.8243182272319353E-5
2	7.060795568079838E-6	0.0	0.0	2.1182386704239514E-5	2.8243182272319353E-5
3	7.060795568079838E-6	0.0	0.0	3.530397784039919E-5	2.8243182272319353E-5
4	7.060795568079838E-6	0.0	0.0	3.530397784039919E-5	3.530397784039919E-5
5	7.060795568079838E-6	7.060795568079838E-6	0.0	5.6486364544638705E-5	3.530397784039919E-5
6	1.4121591136159676E-5	7.060795568079838E-6	0.0	5.6486364544638705E-5	3.530397784039919E-5
7	1.4121591136159676E-5	7.060795568079838E-6	0.0	5.6486364544638705E-5	3.530397784039919E-5
8	1.4121591136159676E-5	7.060795568079838E-6	0.0	5.6486364544638705E-5	4.942556897655886E-5
9	2.1182386704239514E-5	7.060795568079838E-6	0.0	7.766875124887821E-5	7.060795568079838E-5
10-11	2.1182386704239514E-5	7.060795568079838E-6	0.0	9.885113795311774E-5	7.060795568079838E-5
12-13	2.4712784488279435E-5	1.4121591136159676E-5	0.0	1.0591193352119757E-4	1.129727290892774E-4
14-15	3.530397784039919E-5	1.4121591136159676E-5	0.0	1.2709432022543708E-4	2.2947585596259473E-4
16-17	5.6486364544638705E-5	1.4121591136159676E-5	0.0	1.906414803381556E-4	2.8949261829127335E-4
18-19	5.6486364544638705E-5	1.7651988920199596E-5	0.0	2.5065824266683423E-4	3.177358005635927E-4
20-21	6.707755789675846E-5	2.1182386704239514E-5	0.0	3.530397784039919E-4	3.4950938061995197E-4
22-23	7.766875124887821E-5	3.530397784039919E-5	0.0	8.543562637376603E-4	3.9540455181247096E-4
24-25	8.472954681695805E-5	4.236477340847903E-5	0.0	0.0024218528798513844	4.5895171192518946E-4
26-27	1.1297272908927741E-4	4.236477340847903E-5	0.0	0.004328267683232941	4.76603700845389E-4
28-29	1.2003352465735724E-4	4.236477340847903E-5	0.0	0.01523366643813225	4.942556897655887E-4
30-31	1.2003352465735724E-4	5.6486364544638705E-5	0.0	0.04732851269283915	5.083772809017483E-4
32-33	1.2003352465735724E-4	6.001676232867862E-5	0.0	0.10937525374734072	5.366204631740677E-4
34-35	1.2003352465735724E-4	6.354716011271854E-5	0.0	0.19155585336422196	5.648636454463871E-4
36-37	1.2003352465735724E-4	6.354716011271854E-5	0.0	0.31830772500460713	5.683940432304269E-4
38-39	1.2003352465735724E-4	8.472954681695805E-5	0.0	0.5194238955680093	5.931068277187064E-4
40-41	1.2003352465735724E-4	1.0591193352119757E-4	0.0	0.7572879766654828	6.213500099910257E-4
42-43	1.2003352465735724E-4	1.0591193352119757E-4	0.0	0.9768645972416297	6.284108055591056E-4
44-45	1.2003352465735724E-4	1.0591193352119757E-4	0.0	1.2361829056727136	6.601843856154648E-4
46-47	1.2709432022543708E-4	1.0944233130523749E-4	0.0	1.531504210705437	6.919579656718242E-4
48-49	1.3768551357755686E-4	1.1297272908927741E-4	0.0	1.8430935887270161	7.41383534648383E-4
50-51	1.6239829806583627E-4	1.1297272908927741E-4	0.0	2.1706121215501835	7.625659213526224E-4
52-53	1.6239829806583627E-4	1.1297272908927741E-4	0.0	2.496972683900186	8.049306947611014E-4
54-55	1.6239829806583627E-4	1.1650312687331733E-4	0.0	2.834453999269914	8.7200825265786E-4
56-57	1.6239829806583627E-4	1.2356392244139717E-4	0.0	3.214663719019877	9.073122304982591E-4
58-59	1.6239829806583627E-4	1.2709432022543708E-4	0.0	3.6279956307797026	9.214338216344189E-4
60-61	1.6239829806583627E-4	1.2709432022543708E-4	0.0	4.049161495222313	9.284946172024987E-4
62-63	1.6239829806583627E-4	1.2709432022543708E-4	0.0	4.47735991205073	0.0010202849595875366
64-65	1.6592869584987617E-4	1.30624718009477E-4	0.0	4.922401856706802	0.001037936948507736
66-67	1.7651988920199595E-4	1.3415511579351692E-4	0.0	5.4036162570581485	0.0010485281418598559
68-69	1.7651988920199595E-4	1.4121591136159677E-4	0.0	5.926376378532074	0.0010520585396438958
70-71	1.7651988920199595E-4	1.4121591136159677E-4	0.0	6.497630043967574	0.0010767713241321753
72-73	1.9770227590623545E-4	1.4121591136159677E-4	0.0	7.0638140581851925	0.0012144568377097321
74-75	1.9770227590623545E-4	1.4121591136159677E-4	0.0	7.631346684356313	0.001225048031061852
76-77	2.1182386704239514E-4	1.5533750249775642E-4	0.0	8.235164738951797	0.0012356392244139715
78-79	2.1535426482643504E-4	1.6239829806583627E-4	0.0	8.86869462129776	0.0012391696221980115
80-81	2.1888466261047498E-4	1.6239829806583627E-4	0.0	9.546241503215134	0.0012744736000384108
82-83	2.2594545817855482E-4	1.6592869584987617E-4	0.0	10.25512066546586	0.0013733247379915286
84-85	2.2947585596259473E-4	1.7651988920199595E-4	0.0	10.953263888055323	0.0013768551357755683
86-87	2.3653665153067454E-4	1.7651988920199595E-4	0.0	11.669864030259745	0.001387446329127688
88	2.4006704931471448E-4	1.835806847700758E-4	0.0	12.222724323240397	0.0014050983180478877
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATAAG	5300	0.0	21.09439	1
GTACTAG	4585	0.0	20.388237	1
GTATCAA	50050	0.0	18.714876	1
CATGGGG	29520	0.0	18.425846	4
GTACATG	73785	0.0	17.959776	1
TACATGG	73295	0.0	17.636871	2
TAGTACT	5630	0.0	17.353622	4
ACATGGG	69285	0.0	16.962664	3
CTAGTAC	6125	0.0	16.795155	3
GTATAAA	12425	0.0	16.710588	1
TGATCGC	3750	0.0	16.65702	9
ATACGGG	3750	0.0	16.409145	3
TAGGCAT	7150	0.0	15.96253	5
GTGATCG	4010	0.0	15.928376	8
AACGCAG	55880	0.0	15.78419	6
CAACGCA	55705	0.0	15.665815	5
TATAAGG	7005	0.0	15.557054	2
TACGGGC	3965	0.0	15.518985	4
ATCAACG	55825	0.0	15.499114	3
CTAACGC	2250	0.0	15.448815	3
>>END_MODULE