##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765552_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7465406 Sequences flagged as poor quality 0 Sequence length 100 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.2546351799219 33.0 31.0 34.0 31.0 34.0 2 32.21952041188383 34.0 31.0 34.0 31.0 34.0 3 32.245391744266826 34.0 31.0 34.0 30.0 34.0 4 35.715118641906415 37.0 35.0 37.0 35.0 37.0 5 35.74038572048191 37.0 35.0 37.0 35.0 37.0 6 35.88882372907783 37.0 37.0 37.0 35.0 37.0 7 35.8461163398213 37.0 36.0 37.0 35.0 37.0 8 35.906204029626785 37.0 37.0 37.0 35.0 37.0 9 37.58297820640967 39.0 38.0 39.0 35.0 39.0 10-11 37.52603890531874 39.0 38.0 39.0 35.0 39.0 12-13 37.513490625961936 39.0 38.0 39.0 35.0 39.0 14-15 38.83354388227512 41.0 38.5 41.0 35.5 41.0 16-17 38.76608278236978 40.0 38.5 41.0 35.0 41.0 18-19 38.75573103458807 40.0 38.5 41.0 35.0 41.0 20-21 38.770639533871304 40.0 39.0 41.0 35.0 41.0 22-23 38.71571271542365 40.5 39.0 41.0 35.0 41.0 24-25 38.6565651620287 41.0 38.5 41.0 35.0 41.0 26-27 38.49406160897344 40.0 38.0 41.0 34.0 41.0 28-29 38.32041921095785 40.0 38.0 41.0 34.0 41.0 30-31 38.03566195863962 40.0 38.0 41.0 34.0 41.0 32-33 37.63954090373652 40.0 37.0 41.0 33.0 41.0 34-35 37.54373211584206 40.0 37.0 41.0 33.0 41.0 36-37 37.42629088089784 40.0 37.0 41.0 33.0 41.0 38-39 37.21225054605202 40.0 37.0 41.0 32.5 41.0 40-41 36.89566829988885 40.0 36.0 41.0 31.0 41.0 42-43 36.57915168980763 40.0 36.0 41.0 30.5 41.0 44-45 36.08255505460788 39.5 35.0 41.0 30.0 41.0 46-47 35.57204055613318 39.0 35.0 41.0 27.5 41.0 48-49 35.121186510150956 39.0 35.0 41.0 25.0 41.0 50-51 33.912419967514154 38.0 34.0 40.0 23.0 40.5 52-53 33.87014497269137 38.0 34.0 39.5 23.0 40.0 54-55 34.64035505905506 39.0 35.0 40.5 23.0 41.0 56-57 34.61495222095088 39.0 35.0 41.0 22.5 41.0 58-59 34.41962600560505 38.5 35.0 41.0 22.0 41.0 60-61 33.98725896488416 38.0 34.5 40.0 20.5 41.0 62-63 33.44975537834111 37.0 34.0 40.0 16.0 41.0 64-65 33.236775802950305 37.0 34.0 39.0 13.5 41.0 66-67 32.920119280853584 36.0 34.0 39.0 12.5 41.0 68-69 32.67677196658829 36.0 34.0 39.0 8.0 41.0 70-71 32.22731543334683 35.0 33.5 38.5 4.5 40.5 72-73 31.74892155898822 35.0 33.0 37.0 2.0 39.0 74-75 31.328031255098516 35.0 33.0 37.0 2.0 39.0 76-77 30.887867116671217 35.0 32.5 36.0 2.0 38.0 78-79 30.546584539407505 35.0 32.0 36.0 2.0 37.0 80-81 30.231565838482194 35.0 32.0 35.0 2.0 37.0 82-83 29.963395359877282 35.0 32.0 35.0 2.0 36.0 84-85 29.676867272858303 35.0 31.0 35.0 2.0 36.0 86-87 29.37457400173547 35.0 31.0 35.0 2.0 36.0 88-89 29.128215129893807 35.0 31.0 35.0 2.0 35.0 90-91 28.859058167767433 35.0 31.0 35.0 2.0 35.0 92-93 28.503511731311065 35.0 30.0 35.0 2.0 35.0 94-95 28.214742841849457 35.0 29.0 35.0 2.0 35.0 96-97 27.92529200689152 35.0 29.0 35.0 2.0 35.0 98-99 27.544197194365587 35.0 29.0 35.0 2.0 35.0 100 26.91342158751982 34.0 26.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.42821341988678796 1101 2 0.5447996410695808 1101 3 0.5785953464448852 1101 4 0.41791999634048693 1101 5 0.4508225313509229 1101 6 0.4183967923457601 1101 7 0.44594212175017134 1101 8 0.42475651120842883 1101 9 0.5047240585777715 1101 10-11 0.554352479819407 1101 12-13 0.5285001655075732 1101 14-15 0.7630013054577702 1101 16-17 0.7735746805980526 1101 18-19 0.7611377724326545 1101 20-21 0.7585305259496096 1101 22-23 0.7064691646474515 1101 24-25 0.8081136960904942 1101 26-27 0.8092861430241456 1101 28-29 0.875582671501391 1101 30-31 0.8505681998187029 1101 32-33 0.8989494996662017 1101 34-35 1.0096684695808307 1101 36-37 1.134600060762203 1101 38-39 1.0300073355922805 1101 40-41 1.1538852763535061 1101 42-43 1.0908055840274784 1101 44-45 1.2226954020766811 1101 46-47 1.2673611021238784 1101 48-49 1.3884539437625776 1101 50-51 1.3861022089416366 1101 52-53 1.393831920556842 1101 54-55 1.5701804284822813 1101 56-57 1.6318800331138306 1101 58-59 1.6571843109041282 1101 60-61 1.596144335091168 1101 62-63 1.6332047005246437 1101 64-65 1.5464959366674904 1101 66-67 1.5413891610269914 1101 68-69 1.54240185862934 1101 70-71 1.4952560117823026 1101 72-73 1.4842806988087212 1101 74-75 1.429513658696564 1101 76-77 1.392160179200559 1101 78-79 1.479726763895222 1101 80-81 1.4778700426662397 1101 82-83 1.4146443026984095 1101 84-85 1.423903756694937 1101 86-87 1.4249348809450844 1101 88-89 1.4121808602807207 1101 90-91 1.3983520679764183 1101 92-93 1.3960632269412123 1101 94-95 1.4400582843915473 1101 96-97 1.4360323444024736 1101 98-99 1.4454075299882163 1101 100 1.4513630576897505 1102 1 0.4020571826574795 1102 2 0.5035468686476179 1102 3 0.5278020782094615 1102 4 0.37261846394725495 1102 5 0.3994335974551859 1102 6 0.3389374236298437 1102 7 0.4023951251472866 1102 8 0.41881448183156067 1102 9 0.5297536618333893 1102 10-11 0.5285962128405757 1102 12-13 0.5670867883915847 1102 14-15 0.6755588748448318 1102 16-17 0.6764993776119326 1102 18-19 0.6798359920516788 1102 20-21 0.6828101575595369 1102 22-23 0.6906158392713309 1102 24-25 0.686294217665882 1102 26-27 0.7526654722330477 1102 28-29 0.7206741850547616 1102 30-31 0.7801669378716269 1102 32-33 0.7751062319708595 1102 34-35 0.7939008766281788 1102 36-37 0.7467145995950162 1102 38-39 0.862195019557781 1102 40-41 0.9113459493679343 1102 42-43 0.8407948386420259 1102 44-45 0.8527961971676916 1102 46-47 0.8835106457094142 1102 48-49 0.9310288767923893 1102 50-51 1.028488298854512 1102 52-53 1.075267416289286 1102 54-55 1.128434172827177 1102 56-57 1.131711173038326 1102 58-59 1.1553964700447423 1102 60-61 1.1313378915504586 1102 62-63 1.208584648352705 1102 64-65 1.127084696026671 1102 66-67 1.1377170690095895 1102 68-69 1.1636468527424455 1102 70-71 1.1304319199305155 1102 72-73 1.1010569909103758 1102 74-75 1.0513364282470832 1102 76-77 1.0282244944393426 1102 78-79 1.0603680406225422 1102 80-81 1.101427010536753 1102 82-83 1.0827922010585382 1102 84-85 1.0972007762513165 1102 86-87 1.1775257283970681 1102 88-89 1.1332466596203794 1102 90-91 1.0957441798058412 1102 92-93 1.185319833046666 1102 94-95 1.1122589370808846 1102 96-97 1.2752588746803788 1102 98-99 1.3354713789560293 1102 100 1.3619567250459212 1103 1 0.43605479481730214 1103 2 0.5040703570908178 1103 3 0.5631874610408687 1103 4 0.4804975070640367 1103 5 0.47438003852124666 1103 6 0.45108537942361693 1103 7 0.47425594268698035 1103 8 0.4463615813543669 1103 9 0.5766295674319082 1103 10-11 0.5473342668148717 1103 12-13 0.5746999057840227 1103 14-15 0.7134209646323271 1103 16-17 0.7772382751315732 1103 18-19 0.7579615039117087 1103 20-21 0.7286706903475562 1103 22-23 0.6927649689307813 1103 24-25 0.7171676724260649 1103 26-27 0.7299135956229179 1103 28-29 0.7454838439953306 1103 30-31 0.7809551366997027 1103 32-33 0.8289512562923491 1103 34-35 0.8229710747405861 1103 36-37 0.7906714592964335 1103 38-39 0.8156982841223268 1103 40-41 0.8753774135879198 1103 42-43 0.8357946485975845 1103 44-45 0.8572052544968543 1103 46-47 0.8817419068023398 1103 48-49 0.869358601906228 1103 50-51 0.8172024781019189 1103 52-53 0.9081306521182668 1103 54-55 1.028804252881038 1103 56-57 1.056619298099747 1103 58-59 1.1135651419427717 1103 60-61 1.1348321893771853 1103 62-63 1.2035375509298305 1103 64-65 1.2055424181728753 1103 66-67 1.152005589338259 1103 68-69 1.1145243934581615 1103 70-71 1.1573755627234021 1103 72-73 1.0986253932602885 1103 74-75 1.1011885374464185 1103 76-77 1.092837268911822 1103 78-79 1.0617299426755267 1103 80-81 1.0977940642641002 1103 82-83 1.1136889913863897 1103 84-85 1.053690704735807 1103 86-87 1.0396770309679795 1103 88-89 1.0864242105837896 1103 90-91 1.130450416245484 1103 92-93 1.1467841485800783 1103 94-95 1.1831409207416854 1103 96-97 1.2546845162746187 1103 98-99 1.2215850677417706 1103 100 1.3069459985595628 1104 1 0.3492839198058064 1104 2 0.38023215633382534 1104 3 0.4265056354831316 1104 4 0.39454309152686307 1104 5 0.4017521218309099 1104 6 0.3223833128506399 1104 7 0.38809806253379975 1104 8 0.3340503318091095 1104 9 0.42563831889424364 1104 10-11 0.4355370331416353 1104 12-13 0.39631617592355184 1104 14-15 0.5393124989882168 1104 16-17 0.5046101006834505 1104 18-19 0.49659102924490384 1104 20-21 0.4359672623448745 1104 22-23 0.4196810787332481 1104 24-25 0.480171440961918 1104 26-27 0.4574773218396402 1104 28-29 0.4782579796427058 1104 30-31 0.4630854227112451 1104 32-33 0.5111959398917492 1104 34-35 0.4975649099947077 1104 36-37 0.49236923947474764 1104 38-39 0.3518473946529568 1104 40-41 0.39996955584209815 1104 42-43 0.4023486321561549 1104 44-45 0.44443067363150845 1104 46-47 0.3873031714322863 1104 48-49 0.492173410559154 1104 50-51 0.37113890214626366 1104 52-53 0.39055058757337946 1104 54-55 0.49947396030838576 1104 56-57 0.4575439572590341 1104 58-59 0.47168864189503523 1104 60-61 0.4720965943755786 1104 62-63 0.5589967024732445 1104 64-65 0.5255152833087351 1104 66-67 0.4562125125949805 1104 68-69 0.3927060763703949 1104 70-71 0.4058115764056538 1104 72-73 0.4589513514016055 1104 74-75 0.4478421561019843 1104 76-77 0.40531627190473785 1104 78-79 0.38064922289865066 1104 80-81 0.4115179435889402 1104 82-83 0.4352444892718985 1104 84-85 0.4404807358366263 1104 86-87 0.39993639387277113 1104 88-89 0.3801624891953459 1104 90-91 0.37959258078984703 1104 92-93 0.43440686617890023 1104 94-95 0.392012861405842 1104 96-97 0.3872630137693882 1104 98-99 0.40519196604284957 1104 100 0.47380945547798703 1105 1 0.2802103729020615 1105 2 0.34511628414042406 1105 3 0.423697074765343 1105 4 0.35924938850455845 1105 5 0.4170694671021451 1105 6 0.31871752656230257 1105 7 0.3890389275422237 1105 8 0.3708777182182743 1105 9 0.4846181569018313 1105 10-11 0.4736104311013065 1105 12-13 0.4845494370225012 1105 14-15 0.5474130635072285 1105 16-17 0.5969920310405641 1105 18-19 0.6127882353897931 1105 20-21 0.5880133867572255 1105 22-23 0.5468429599597897 1105 24-25 0.614251081985735 1105 26-27 0.5209834442800556 1105 28-29 0.5474252983767229 1105 30-31 0.5661434332799757 1105 32-33 0.5687479077366078 1105 34-35 0.554005608227726 1105 36-37 0.535873001885875 1105 38-39 0.553474335908156 1105 40-41 0.6210319256471521 1105 42-43 0.6132379016798879 1105 44-45 0.5896902244077964 1105 46-47 0.5952797767242046 1105 48-49 0.6858297252632894 1105 50-51 0.6299965850380431 1105 52-53 0.7022965687057336 1105 54-55 0.745113538733726 1105 56-57 0.7267119682683045 1105 58-59 0.7291015987704839 1105 60-61 0.7811926821812563 1105 62-63 0.8351717243428496 1105 64-65 0.776166158407591 1105 66-67 0.7608316877804739 1105 68-69 0.762005661634582 1105 70-71 0.7311084576007332 1105 72-73 0.6916625425555871 1105 74-75 0.6988210857605281 1105 76-77 0.7305767771817635 1105 78-79 0.7649690100135196 1105 80-81 0.741751261328595 1105 82-83 0.7313440741165209 1105 84-85 0.7059417072456569 1105 86-87 0.6838722933795758 1105 88-89 0.7627910793243373 1105 90-91 0.8616327054833093 1105 92-93 0.8427399302940763 1105 94-95 0.7601003670961362 1105 96-97 0.6360996451999448 1105 98-99 0.5651855887192703 1105 100 0.6705243786249433 1106 1 0.2139483567595093 1106 2 0.2770508253143831 1106 3 0.325233648550288 1106 4 0.2846510652592187 1106 5 0.3231846602187076 1106 6 0.2653458478140678 1106 7 0.3049995022845806 1106 8 0.3019979419723171 1106 9 0.33182287326647497 1106 10-11 0.34571563577627984 1106 12-13 0.33876897466451084 1106 14-15 0.39713006388711136 1106 16-17 0.4138790834636126 1106 18-19 0.4378568240271363 1106 20-21 0.4415531074724157 1106 22-23 0.4353222087552808 1106 24-25 0.4550040155126922 1106 26-27 0.44539191056669836 1106 28-29 0.45624968236290897 1106 30-31 0.4486917433515032 1106 32-33 0.47151529526159663 1106 34-35 0.42823072275740515 1106 36-37 0.45767388217351623 1106 38-39 0.42785805363718765 1106 40-41 0.4728869275014418 1106 42-43 0.4117820554255047 1106 44-45 0.4104775965973104 1106 46-47 0.34070746027943954 1106 48-49 0.3020787504556992 1106 50-51 0.31648395039553634 1106 52-53 0.3640076359681217 1106 54-55 0.39152954218366887 1106 56-57 0.39800669172485925 1106 58-59 0.37304414710078504 1106 60-61 0.32775469472883145 1106 62-63 0.30903861313051095 1106 64-65 0.26871970610029194 1106 66-67 0.21008146527301363 1106 68-69 0.2359460257422441 1106 70-71 0.25777568011457674 1106 72-73 0.2354317148091205 1106 74-75 0.26230677750702824 1106 76-77 0.24135298236765124 1106 78-79 0.2333039483782926 1106 80-81 0.3035187355377289 1106 82-83 0.29198502401915505 1106 84-85 0.3042545839501436 1106 86-87 0.3873799043878101 1106 88-89 0.3429979992093557 1106 90-91 0.3868205781998526 1106 92-93 0.44663278775374593 1106 94-95 0.32603476929532604 1106 96-97 0.34024713539817597 1106 98-99 0.431267334516221 1106 100 0.5064235834807498 1107 1 0.15863872364677434 1107 2 0.2254539998449232 1107 3 0.2558301336192699 1107 4 0.19617184465933235 1107 5 0.29726669901376823 1107 6 0.26179286727942497 1107 7 0.25456553398088744 1107 8 0.22230770419739798 1107 9 0.3185223886647961 1107 10-11 0.30502166757410976 1107 12-13 0.35187042025933124 1107 14-15 0.38278943402631427 1107 16-17 0.3724022567022942 1107 18-19 0.35549934952940276 1107 20-21 0.33872369313411355 1107 22-23 0.3196236406958235 1107 24-25 0.39267017409222404 1107 26-27 0.37495871546572346 1107 28-29 0.3215305296753783 1107 30-31 0.37432003309550765 1107 32-33 0.39956256553597314 1107 34-35 0.412577082852259 1107 36-37 0.4049618350056434 1107 38-39 0.4302968056453196 1107 40-41 0.3988360669474105 1107 42-43 0.3815476035868812 1107 44-45 0.44726089524197477 1107 46-47 0.4398344217111898 1107 48-49 0.5078354801333589 1107 50-51 0.3753107362859964 1107 52-53 0.35354184790791265 1107 54-55 0.35682804220716946 1107 56-57 0.47342858931147447 1107 58-59 0.3798692980095808 1107 60-61 0.43769577167882545 1107 62-63 0.4805657466237818 1107 64-65 0.5167038797956067 1107 66-67 0.4588090649243881 1107 68-69 0.3773206256870836 1107 70-71 0.4135177439537614 1107 72-73 0.41346905336443385 1107 74-75 0.3777283609570432 1107 76-77 0.38845636339702594 1107 78-79 0.3747025315854593 1107 80-81 0.3662343779878974 1107 82-83 0.37382437544700053 1107 84-85 0.44693834999621274 1107 86-87 0.4468589467317301 1107 88-89 0.4959694628791631 1107 90-91 0.43140943556146283 1107 92-93 0.5373693777443549 1107 94-95 0.4247827988606616 1107 96-97 0.4743025549791007 1107 98-99 0.429803748575619 1107 100 0.4026515018667389 1108 1 0.09245212225282273 1108 2 0.07173306346991382 1108 3 0.14860502002506593 1108 4 0.1580247122766778 1108 5 0.24618219972307998 1108 6 0.22117706152818783 1108 7 0.26270168981925224 1108 8 0.22008684676026746 1108 9 0.29865851798483334 1108 10-11 0.32388835021995277 1108 12-13 0.32323122082521394 1108 14-15 0.36886959287958376 1108 16-17 0.3428652424528238 1108 18-19 0.3968687237683426 1108 20-21 0.31864794027355003 1108 22-23 0.3107187000660261 1108 24-25 0.3575050757862641 1108 26-27 0.38305244902510793 1108 28-29 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-1.0657890989832133 2316 74-75 -1.0355777755960034 2316 76-77 -0.9807916327081045 2316 78-79 -0.9494065302400401 2316 80-81 -0.8427710811533586 2316 82-83 -0.8999050465768406 2316 84-85 -0.911264814615155 2316 86-87 -0.9330453091214821 2316 88-89 -0.870254451589286 2316 90-91 -0.7965533632134978 2316 92-93 -0.7728732879424918 2316 94-95 -0.7021081380266097 2316 96-97 -0.7708880401200737 2316 98-99 -0.835017064041061 2316 100 -0.9532756117537389 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 20707.0 3 3637.0 4 3351.0 5 3889.0 6 4848.0 7 5722.0 8 7222.0 9 9077.0 10 12423.0 11 19340.0 12 31750.0 13 45490.0 14 46581.0 15 54638.0 16 71888.0 17 97917.0 18 95811.0 19 54189.0 20 45711.0 21 41609.0 22 42297.0 23 48594.0 24 54027.0 25 56814.0 26 61590.0 27 71626.0 28 84792.0 29 101805.0 30 123606.0 31 152320.0 32 189746.0 33 253131.0 34 359481.0 35 559590.0 36 1003931.0 37 1891210.0 38 1508138.0 39 226578.0 40 330.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.94166428961585 16.407819578557273 13.336750385793 23.31376574603388 2 12.593771238310737 23.016208171241537 36.23815276189631 28.151867828551413 3 20.4041854835988 29.492462851419525 28.85625815744009 21.247093507541585 4 13.437874923074173 19.440328213281084 32.79104854290347 34.33074832074127 5 12.018724085763193 37.31836857535182 33.80721285749219 16.855694481392806 6 30.126997485106678 31.13422909445464 19.481577055008813 19.25719636542987 7 22.89444279957774 33.20112093007844 22.735753035852298 21.168683234491525 8 28.639745921383696 30.169944581247755 22.4050424090258 18.78526708834275 9 28.823003862863384 15.03724740983587 19.042474852729843 37.0972738745709 10-11 24.03547206646244 27.192228527186142 24.753773962524406 24.018525443827013 12-13 28.052442637425244 24.513325789568153 25.543447106898032 21.890784466108567 14-15 27.702955869689365 21.88641761128832 25.55930406659638 24.851322452425933 16-17 22.782819354512895 25.736978917437693 29.782626001730716 21.697575726318693 18-19 24.966435371748506 25.11411638210771 30.50434581021877 19.415102435925018 20-21 22.822432069387077 24.866057249034473 30.35948261549839 21.952028066080057 22-23 21.161128098143163 27.12907396111044 25.51472786747821 26.19507007326819 24-25 21.830714658898483 28.44216508098768 24.328669813669666 25.398450446444176 26-27 23.942838672419462 24.45740789143891 25.506900997150616 26.09285243899101 28-29 22.70414994204741 23.529455178579518 30.0263579440334 23.740036935339674 30-31 25.6317246568598 24.077730899952442 28.255817114504467 22.03472732868329 32-33 19.840304533168773 26.06557109668038 29.497341597103677 24.59678277304717 34-35 19.128591819703306 27.268341930425123 30.22659947084227 23.3764667790293 36-37 23.304892442000387 26.66220606017703 27.418974028329878 22.613927469492708 38-39 24.74194131134321 24.33906469373179 26.57262279703034 24.346371197894655 40-41 22.25251980535335 26.52230496540662 26.708559244123748 24.51661598511628 42-43 26.007519300438165 24.722349365494935 26.208957140604134 23.06117419346277 44-45 25.96741544906899 23.618871960609248 27.711315482817096 22.70239710750467 46-47 23.29581791169168 24.45282418010568 30.984741084433807 21.266616823768825 48-49 23.787541564801284 26.242527196303335 29.364778207058745 20.60515303183664 50-51 21.184796317402917 27.290453391619067 27.274397349927842 24.25035294105017 52-53 20.22507295862521 28.39584894337807 23.934458765176316 27.444619332820402 54-55 22.02024805102289 27.32036003385621 23.37140034834636 27.28799156677454 56-57 24.04798218195333 24.13440480223972 24.46690467405354 27.350708341753403 58-59 23.349542743373476 23.94854174983583 28.222738293815997 24.479177212974697 60-61 23.696647963129728 25.111727188716692 26.646493767208202 24.545131080945385 62-63 19.53784920920105 25.955200126570737 28.256380827751173 26.250569836477045 64-65 19.480864373670485 26.48959290968364 28.230425895273658 25.799116821372216 66-67 23.451355674713156 25.561933571201585 25.838968290043724 25.147742464041535 68-69 24.300739698252343 24.111576767349103 25.628171695157416 25.959511839241138 70-71 22.193057180730587 25.566941783866476 26.269077751719095 25.970923283683838 72-73 24.83323184707275 24.117562542836527 26.191014716379772 24.85819089371095 74-75 25.3254679528716 23.106473202662098 27.11824939699078 24.44980944747552 76-77 23.070391152625717 25.105358638106484 29.136520180770876 22.687730028496926 78-79 23.464343220799446 25.2928836163607 28.436717095435977 22.806056067403883 80-81 21.39800569326922 25.87753357452144 27.572133871197828 25.15232686101151 82-83 21.319279715420674 27.049809898859746 24.500825650499458 27.130084735220123 84-85 22.31203975557878 26.86928171263635 24.20285010507558 26.615828426709285 86-87 23.188450524647404 24.0844685072678 25.545784524957902 27.18129644312689 88-89 23.484344903769543 23.354196866666385 27.49676977817813 25.664688451385942 90-91 24.33756040584083 24.724287955520527 26.162353240192722 24.775798398445925 92-93 21.912546009732186 25.333882725951014 27.165429080684955 25.588142183631845 94-95 21.4743759050078 25.937582368898955 27.591223912623004 24.99681781347024 96-97 24.32390855484288 25.12327106488767 25.36383521419618 25.18898516607327 98-99 25.21021595732529 24.0274383534944 24.922660119420723 25.839685569759585 100 24.403869057263883 25.007331834739034 26.01439849977416 24.574400608222923 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1805.0 1 2166.0 2 1744.5 3 857.5 4 664.5 5 677.0 6 881.0 7 1369.0 8 1861.5 9 1918.0 10 1662.0 11 1538.0 12 1699.0 13 1949.0 14 2293.5 15 2723.0 16 3289.0 17 3971.0 18 4816.5 19 5903.5 20 7301.5 21 9032.5 22 11297.0 23 14387.5 24 18188.5 25 22626.5 26 28267.5 27 34675.5 28 41264.0 29 49350.5 30 57156.5 31 63258.5 32 69172.0 33 74757.5 34 80132.5 35 83512.5 36 87139.5 37 92141.0 38 93241.0 39 94523.0 40 95125.5 41 95817.5 42 99732.5 43 110328.0 44 128335.0 45 147928.0 46 182717.5 47 245352.0 48 474106.0 49 782049.0 50 1084911.0 51 941517.5 52 458631.5 53 271901.5 54 210265.5 55 167046.0 56 145512.5 57 124930.0 58 110952.0 59 102143.0 60 86225.0 61 68651.5 62 54449.0 63 42492.0 64 31122.0 65 24624.5 66 19963.5 67 14834.5 68 11230.0 69 9033.5 70 7711.5 71 6812.5 72 5990.0 73 6227.0 74 5227.0 75 4107.0 76 3282.0 77 2342.0 78 1773.5 79 1311.0 80 1004.5 81 809.0 82 674.0 83 557.5 84 382.5 85 253.0 86 175.0 87 108.5 88 75.5 89 61.5 90 47.5 91 47.5 92 49.0 93 34.0 94 22.5 95 21.0 96 20.5 97 17.0 98 11.5 99 9.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11636339671278427 2 0.36824520997250515 3 0.37743426144539227 4 0.3756928960059238 5 0.30254215242948607 6 0.13688471866098106 7 0.1505477398014254 8 0.1265302918555267 9 0.20751717990957225 10-11 0.2082137260853596 12-13 0.22006840619250984 14-15 0.14985119362563804 16-17 0.13557869458137975 18-19 0.1540773535960402 20-21 0.15496143143453953 22-23 0.16303868804991986 24-25 0.17588460694569055 26-27 0.16837664287782875 28-29 0.17672849942789448 30-31 0.21932497710104448 32-33 0.21569489991569113 34-35 0.1310578419981445 36-37 0.09080551010889429 38-39 0.08436915554224378 40-41 0.0836993996039867 42-43 0.07416207504320596 44-45 0.09656541117790512 46-47 0.14265131728937447 48-49 0.22296844940516294 50-51 0.1792601768745062 52-53 0.11075753950957257 54-55 0.059487722435993436 56-57 0.07438979206221337 58-59 0.11004090065563749 60-61 0.0855814137904891 62-63 0.09545361632039838 64-65 0.10445513613057347 66-67 0.10040981026350074 68-69 0.0905041199366786 70-71 0.12553235550752365 72-73 0.14995835457575918 74-75 0.19345230520617365 76-77 0.19325137842469653 78-79 0.15527621672552033 80-81 0.09073853451506857 82-83 0.13086361277604996 84-85 0.16022571310924014 86-87 0.1724889443387272 88-89 0.16034626917812642 90-91 0.18108861058594802 92-93 0.10010172253190248 94-95 0.34228546980566094 96-97 0.3429485281845354 98-99 0.3829597479360131 100 0.38389070869019043 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7465406.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.888355215583225 #Duplication Level Percentage of deduplicated Percentage of total 1 79.50009392094655 20.58126671097693 2 11.53478648014475 5.972332994677883 3 3.3823153637486056 2.626877447635455 4 1.4742723310617185 1.5266594316412672 5 0.7997766614938109 1.035245115294252 6 0.5372738902841859 0.8345482391841176 7 0.3579399507830036 0.6486533614203142 8 0.2867193392895249 0.5938153682163638 9 0.22304820609774903 0.5196916070691429 >10 1.457892694372528 7.44141589490201 >50 0.20088369995440045 3.6752523965654635 >100 0.19882060246594513 10.884845283848888 >500 0.027233852417900155 4.759071930879463 >1k 0.014797584367913123 6.716867788504071 >5k 0.0013118425858079006 2.470640919900624 >10k+ 0.002833579985345065 29.712815509283836 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 240129 3.2165564739546646 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 212203 2.842484387319323 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 102825 1.3773530870256754 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 94807 1.269951024766771 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 90060 1.206364395988644 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 88023 1.179078539064051 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 84336 1.1296907361769741 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 80028 1.0719845645367445 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 77301 1.0354560756642035 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 76348 1.0226905274810238 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 63192 0.8464643450068221 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 53564 0.7174961415360397 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 46015 0.6163763899779864 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 40419 0.5414173053682546 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 37075 0.4966240282176214 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 36224 0.48522478214848597 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 34059 0.4562243500219546 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 33181 0.4444634357461604 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 30856 0.41331978461720636 No Hit CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC 29269 0.39206173113692677 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 28788 0.3856186790108937 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC 27321 0.3659680397824311 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 27147 0.3636372891172965 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26796 0.35893560243073186 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 25026 0.3352262422164313 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 24083 0.3225946452209029 No Hit GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG 23434 0.31390121314232605 No Hit GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA 22885 0.3065472929402634 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 21992 0.294585451882992 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 21406 0.2867359122866191 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 20966 0.2808420600299569 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 20614 0.276126978224627 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 20351 0.2726040619893948 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 19914 0.266750395089028 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19608 0.2626514887468947 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 19060 0.2553109636635971 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 18949 0.2538241054806664 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 18090 0.24231769846140985 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 17907 0.23986639172738897 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT 16945 0.22698028747532287 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 16804 0.22509157572943786 No Hit TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA 16495 0.22095248403100914 No Hit CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 15916 0.21319671026599224 No Hit GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG 15620 0.20923175511151035 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 14178 0.1899159938521763 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT 14163 0.18971506707069916 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 14111 0.1890185208949118 No Hit ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC 14019 0.1877861699685188 No Hit ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG 13661 0.18299071745059814 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13435 0.17996342060967616 No Hit TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG 12827 0.1718191884004701 No Hit TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG 12271 0.16437150236705145 No Hit CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA 12093 0.16198717122685624 No Hit GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA 10457 0.14007275692708476 No Hit TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA 9673 0.1295709838152138 No Hit ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACT 9434 0.12636955043034498 No Hit CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT 8777 0.11756895740164701 No Hit GAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAG 8686 0.11635000159401912 No Hit GTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACT 8547 0.11448808008566447 No Hit GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA 8467 0.11341647058445314 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 8222 0.11013466648699347 No Hit TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC 8031 0.10757619880285144 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT 7896 0.10576785776955736 No Hit GATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC 7798 0.10445513613057347 No Hit CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC 7691 0.10302185842270334 No Hit CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTG 7554 0.10118672715187894 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.545072565376886E-4 0.0 0.0 0.0 0.0 2 3.616682066588207E-4 0.0 0.0 0.0 0.0 3 3.616682066588207E-4 0.0 0.0 1.3395118765141508E-5 0.0 4 5.625949881359433E-4 0.0 0.0 2.6790237530283016E-5 0.0 5 0.002585257921672311 1.3395118765141508E-5 0.0 5.358047506056603E-5 0.0 6 0.005277676793465754 1.3395118765141508E-5 0.0 5.358047506056603E-5 2.6790237530283016E-5 7 0.006081383919374244 1.3395118765141508E-5 0.0 5.358047506056603E-5 2.6790237530283016E-5 8 0.006818115451457028 1.3395118765141508E-5 0.0 5.358047506056603E-5 2.6790237530283016E-5 9 0.007434290914653537 1.3395118765141508E-5 0.0 1.0716095012113206E-4 2.6790237530283016E-5 10-11 0.012644992114293582 1.3395118765141508E-5 0.0 2.2771701900740562E-4 2.6790237530283016E-5 12-13 0.024573345374652097 1.3395118765141508E-5 0.0 3.5497064727625E-4 3.348779691285377E-5 14-15 0.03753982033930907 1.3395118765141508E-5 0.0 5.69292547518514E-4 4.688291567799528E-5 16-17 0.04241564356982058 1.3395118765141508E-5 0.0 8.974729572644811E-4 5.358047506056603E-5 18-19 0.04503438928840575 1.3395118765141508E-5 0.0 0.0013395118765141506 5.358047506056603E-5 20-21 0.05506733324349673 1.3395118765141508E-5 0.0 0.0021633116805703533 6.697559382570753E-5 22-23 0.058945220126005206 1.3395118765141508E-5 0.0 0.004782057399155518 6.697559382570753E-5 24-25 0.08004253218110308 1.3395118765141508E-5 0.0 0.010528563349401224 6.697559382570753E-5 26-27 0.14751374540112083 2.009267814771226E-5 0.0 0.021191077886453864 6.697559382570753E-5 28-29 0.16306547828745016 3.348779691285377E-5 0.0 0.05773296187775989 6.697559382570753E-5 30-31 0.1668964822542806 4.018535629542452E-5 0.0 0.15015258379785373 8.037071259084904E-5 32-33 0.16913346708805926 4.018535629542452E-5 0.0 0.2979141388961297 9.376583135599055E-5 34-35 0.17112933978406533 4.688291567799528E-5 0.0 0.49758847676871154 9.376583135599055E-5 36-37 0.17419012442190018 5.358047506056603E-5 0.0 0.7870636908428021 9.376583135599055E-5 38-39 0.17729109441603041 6.0278034443136787E-5 0.0 1.2260887083703151 1.0716095012113206E-4 40-41 0.18041885464769097 6.697559382570753E-5 0.0 1.8916117890976056 1.0716095012113206E-4 42-43 0.1883353698378896 6.697559382570753E-5 0.0 2.3532611622194426 1.1385850950370281E-4 44-45 0.19716275310411785 6.697559382570753E-5 0.0 2.792540687003493 1.2055606888627356E-4 46-47 0.20124156676810342 6.697559382570753E-5 0.0 3.3039261361003005 1.2055606888627356E-4 48-49 0.2038871027242189 6.697559382570753E-5 0.0 3.7568485893466477 1.2055606888627356E-4 50-51 0.20798600906635217 6.697559382570753E-5 0.0 4.269667316151326 1.2055606888627356E-4 52-53 0.20962021355569946 6.697559382570753E-5 0.0 4.918145108249973 1.473463064165566E-4 54-55 0.21422813441090813 6.697559382570753E-5 0.0 5.463808666266778 1.473463064165566E-4 56-57 0.22413382473773025 6.697559382570753E-5 0.0 6.017248090726747 1.473463064165566E-4 58-59 0.22762325317604964 6.697559382570753E-5 0.0 6.596473118809613 1.473463064165566E-4 60-61 0.23017502330080908 6.697559382570753E-5 0.0 7.0880672799309234 1.473463064165566E-4 62-63 0.23186280826521694 6.697559382570753E-5 0.0 7.598957109633421 1.473463064165566E-4 64-65 0.2334501298388862 6.697559382570753E-5 0.0 8.182897219521617 1.473463064165566E-4 66-67 0.23550628056933542 6.697559382570753E-5 0.0 8.773206708382638 1.6743898456426882E-4 68-69 0.2379174019470609 6.697559382570753E-5 0.0 9.534183405430328 1.7413654394683958E-4 70-71 0.2407504695658883 6.697559382570753E-5 0.0 10.488753056431225 1.7413654394683958E-4 72-73 0.24758867769549306 6.697559382570753E-5 0.0 11.20529814453494 1.7413654394683958E-4 74-75 0.25450055897830604 6.697559382570753E-5 0.0 11.874109726919071 1.7413654394683958E-4 76-77 0.25671745113393696 6.697559382570753E-5 0.0 12.594894370111955 1.7413654394683958E-4 78-79 0.2586463482361174 6.697559382570753E-5 0.0 13.25532730570849 1.7413654394683958E-4 80-81 0.2615865768050659 8.037071259084904E-5 0.0 14.025144781141172 1.7413654394683958E-4 82-83 0.26308683010676176 8.037071259084904E-5 0.0 14.983123489867797 1.875316627119811E-4 84-85 0.2681166972030724 1.0716095012113205E-4 0.0 15.839547105676502 1.875316627119811E-4 86-87 0.27671636345029327 1.3395118765141507E-4 0.0 16.65378949249378 1.875316627119811E-4 88 0.27881939709642045 1.3395118765141507E-4 0.0 17.31861602704528 1.875316627119811E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCTAC 10285 0.0 31.552305 1 ATCTACA 11005 0.0 29.523651 2 TCTACAC 13315 0.0 26.419909 3 AGTACTC 3100 0.0 25.196438 5 TACACTC 13910 0.0 25.065931 5 CTACACT 15420 0.0 24.24297 4 GATGTAA 2300 0.0 23.754522 1 TATCACG 400 0.0 23.544043 2 GTGATCG 1240 0.0 22.373817 8 CACTCTT 16135 0.0 21.802364 7 CGTTAAC 655 0.0 21.572285 1 ACACTCT 16250 0.0 21.559078 6 GTATAAG 1650 0.0 21.408857 1 ATTGCGT 1105 0.0 21.278204 6 ACTCTTT 18665 0.0 20.230064 8 TGTAAGA 2565 0.0 20.195621 3 GTACTAG 1665 0.0 20.084465 1 AAGTACT 3985 0.0 19.966782 4 GCATAGA 1330 0.0 19.123098 1 TGATCGC 1465 0.0 18.937567 9 >>END_MODULE