##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765552_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7465406 Sequences flagged as poor quality 0 Sequence length 100 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.469561601874034 34.0 31.0 34.0 31.0 34.0 2 32.56867530044582 34.0 31.0 34.0 31.0 34.0 3 32.61614974456848 34.0 31.0 34.0 31.0 34.0 4 36.11772835931495 37.0 35.0 37.0 35.0 37.0 5 36.05370210273895 37.0 35.0 37.0 35.0 37.0 6 36.15384856496753 37.0 36.0 37.0 35.0 37.0 7 36.09715399269645 37.0 36.0 37.0 35.0 37.0 8 36.13997872319335 37.0 36.0 37.0 35.0 37.0 9 37.85776326163641 39.0 38.0 39.0 35.0 39.0 10-11 37.81331698503738 39.0 38.0 39.0 35.0 39.0 12-13 37.78540122265286 39.0 38.0 39.0 35.0 39.0 14-15 39.08542281558431 40.5 38.5 41.0 36.0 41.0 16-17 39.02868571381114 40.0 38.5 41.0 36.0 41.0 18-19 39.06502345619247 40.0 38.5 41.0 35.5 41.0 20-21 39.08993268417016 40.0 39.0 41.0 36.0 41.0 22-23 39.068300036193605 40.0 39.0 41.0 35.5 41.0 24-25 39.01980361148476 40.0 39.0 41.0 35.0 41.0 26-27 38.83421203079913 40.0 38.0 41.0 35.0 41.0 28-29 38.67761873902103 40.0 38.0 41.0 35.0 41.0 30-31 38.43943644860039 40.0 38.0 41.0 34.5 41.0 32-33 38.04611691581141 40.0 37.0 41.0 34.0 41.0 34-35 38.0268768369731 40.0 37.5 41.0 34.0 41.0 36-37 37.95545413069296 40.0 37.5 41.0 33.5 41.0 38-39 37.77344534242344 40.0 37.0 41.0 33.0 41.0 40-41 37.47471818679386 40.0 37.0 41.0 33.0 41.0 42-43 37.161102557583604 40.0 36.5 41.0 32.5 41.0 44-45 36.71113627309754 40.0 36.0 41.0 31.0 41.0 46-47 36.29990150569172 39.0 35.0 41.0 30.0 41.0 48-49 35.96461451661169 39.0 35.0 41.0 30.0 41.0 50-51 35.73428002442198 39.0 35.0 41.0 28.5 41.0 52-53 35.618126328293464 39.0 35.0 41.0 28.0 41.0 54-55 35.598472742138874 39.0 35.0 41.0 28.0 41.0 56-57 35.407938764482466 39.0 35.0 41.0 28.0 41.0 58-59 35.31304064373726 39.0 35.0 41.0 28.0 41.0 60-61 34.90160227052621 38.0 35.0 40.5 27.0 41.0 62-63 34.45962811667577 37.0 34.5 40.0 26.0 41.0 64-65 34.313407268137865 37.0 35.0 39.5 26.0 41.0 66-67 33.90836948452636 37.0 34.5 39.0 26.0 41.0 68-69 33.545521435806705 36.0 34.0 39.0 26.0 41.0 70-71 33.08242398337077 35.0 34.0 38.5 25.0 40.5 72-73 32.60842076104099 35.0 34.0 37.0 24.0 39.0 74-75 32.2134266374796 35.0 33.0 37.0 22.5 39.0 76-77 30.731128019025356 34.0 31.0 35.0 20.0 37.0 78-79 31.442420948036855 35.0 33.0 36.0 20.0 37.0 80-81 31.412376634304955 35.0 33.0 35.0 20.0 37.0 82-83 31.272890918457747 35.0 33.0 35.0 20.0 36.0 84-85 31.082340531780858 35.0 33.0 35.0 19.0 36.0 86-87 30.87901943980006 35.0 33.0 35.0 17.5 36.0 88-89 30.717820169458967 35.0 33.0 35.0 12.5 35.0 90-91 30.572790414881656 35.0 33.0 35.0 6.5 35.0 92-93 30.373166174753255 35.0 32.5 35.0 2.0 35.0 94-95 30.317998177192237 35.0 33.0 35.0 2.0 35.0 96-97 30.23193165917567 35.0 33.0 35.0 2.0 35.0 98-99 30.077829189731943 35.0 33.0 35.0 2.0 35.0 100 29.71360807436327 35.0 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4189548989787113 1101 2 0.4070530087185844 1101 3 0.42944756438499354 1101 4 0.2721094943652034 1101 5 0.3428278361810868 1101 6 0.30603540706604093 1101 7 0.286751478067778 1101 8 0.2918312771337028 1101 9 0.3694514488820957 1101 10-11 0.38151062535328606 1101 12-13 0.417276167492993 1101 14-15 0.5313932459259121 1101 16-17 0.49057007051174395 1101 18-19 0.4960102878363628 1101 20-21 0.4567749857865664 1101 22-23 0.515874969064626 1101 24-25 0.5511122044886037 1101 26-27 0.6004019836729739 1101 28-29 0.6623282893395839 1101 30-31 0.6479859174387101 1101 32-33 0.7158069699569651 1101 34-35 0.6654222464940815 1101 36-37 0.7380447843860551 1101 38-39 0.7589804804400444 1101 40-41 0.7694894328002349 1101 42-43 0.9179391622175714 1101 44-45 0.9965808043805708 1101 46-47 1.078724773096262 1101 48-49 1.0970712821940865 1101 50-51 1.1051708192847727 1101 52-53 1.0724707984172 1101 54-55 1.2196950793509487 1101 56-57 1.2126267376463318 1101 58-59 1.264924114755381 1101 60-61 1.2981666977287603 1101 62-63 1.3152397920486933 1101 64-65 1.2565909080448776 1101 66-67 1.288632470982357 1101 68-69 1.2794013502898949 1101 70-71 1.2717132397198512 1101 72-73 1.304762707251136 1101 74-75 1.2649743957568873 1101 76-77 1.2949563723533686 1101 78-79 1.2931535249157555 1101 80-81 1.2653152617463945 1101 82-83 1.2738790330825402 1101 84-85 1.2587036102340399 1101 86-87 1.291653501328998 1101 88-89 1.3114079353910455 1101 90-91 1.2766520636705678 1101 92-93 1.3854014971102977 1101 94-95 1.3595102660996439 1101 96-97 1.5001673418757555 1101 98-99 1.4795493311642147 1101 100 1.5718537527078205 1102 1 0.42769314769016376 1102 2 0.30519840019717037 1102 3 0.3743466342734294 1102 4 0.2804856067094974 1102 5 0.26552508320207835 1102 6 0.2723276955023408 1102 7 0.31092611994827024 1102 8 0.3161933162860606 1102 9 0.36293912645662374 1102 10-11 0.386445851494706 1102 12-13 0.380544079994273 1102 14-15 0.49102362041890757 1102 16-17 0.5100248158989942 1102 18-19 0.4738429514677662 1102 20-21 0.4620847251788973 1102 22-23 0.5212326970108236 1102 24-25 0.5627949419762501 1102 26-27 0.5933394806350165 1102 28-29 0.6377085578414494 1102 30-31 0.6814343614168834 1102 32-33 0.7489435164643368 1102 34-35 0.7971055386911488 1102 36-37 0.8461683126620585 1102 38-39 0.8257147530213302 1102 40-41 0.8879211893635812 1102 42-43 0.9453593605717572 1102 44-45 1.0160380532738529 1102 46-47 1.104258762494446 1102 48-49 1.0813896016923081 1102 50-51 1.1311108267289072 1102 52-53 1.14725463051645 1102 54-55 1.1905027362763363 1102 56-57 1.2614140787046395 1102 58-59 1.2090940174145643 1102 60-61 1.2619881100977324 1102 62-63 1.2492295671674682 1102 64-65 1.2136921225997312 1102 66-67 1.231590845496953 1102 68-69 1.2333795113910213 1102 70-71 1.2282191824243256 1102 72-73 1.2532889917016306 1102 74-75 1.256893781512396 1102 76-77 1.197273589408809 1102 78-79 1.1930870666758118 1102 80-81 1.1653206456908265 1102 82-83 1.1890565637277 1102 84-85 1.1831160120515953 1102 86-87 1.1929047592704514 1102 88-89 1.1959985013251782 1102 90-91 1.2048127484767974 1102 92-93 1.2729982393344663 1102 94-95 1.30793410370773 1102 96-97 1.3482980273660878 1102 98-99 1.3646797823938925 1102 100 1.4352427730167108 1103 1 0.3727966841127568 1103 2 0.30964630639039115 1103 3 0.3443767043441781 1103 4 0.2692129788211375 1103 5 0.30644401423253953 1103 6 0.27567218895935497 1103 7 0.2786733205201273 1103 8 0.26875368217096707 1103 9 0.311903819978987 1103 10-11 0.35134254291544664 1103 12-13 0.357464586713661 1103 14-15 0.48550364307304505 1103 16-17 0.42239895870405775 1103 18-19 0.40975602786941323 1103 20-21 0.37558161327989126 1103 22-23 0.46657292276262297 1103 24-25 0.47602155485508746 1103 26-27 0.5107083798934013 1103 28-29 0.5530957712943305 1103 30-31 0.6128124497965786 1103 32-33 0.5696955795490126 1103 34-35 0.608214801856299 1103 36-37 0.6904914777777122 1103 38-39 0.7469952341550652 1103 40-41 0.7797710704230596 1103 42-43 0.7361675723396459 1103 44-45 0.8632904621134188 1103 46-47 0.8368944621015899 1103 48-49 0.7674164160182642 1103 50-51 0.8525015388894772 1103 52-53 1.0020400965894183 1103 54-55 1.0632853061139897 1103 56-57 0.9894225111130837 1103 58-59 0.9553590666245313 1103 60-61 1.0054916613038927 1103 62-63 0.9777541382340118 1103 64-65 0.9804843078462397 1103 66-67 0.958223962494607 1103 68-69 0.9133300090134711 1103 70-71 0.877995360468887 1103 72-73 0.9879343719207085 1103 74-75 1.032179811523882 1103 76-77 0.9558253399806205 1103 78-79 0.9609709968746891 1103 80-81 0.9314820750056327 1103 82-83 0.9690770103048401 1103 84-85 0.9982474713577254 1103 86-87 0.9583481802048261 1103 88-89 0.998188354043883 1103 90-91 1.0413118704924713 1103 92-93 1.0174317220994311 1103 94-95 1.038512874084482 1103 96-97 1.071342165555489 1103 98-99 1.0522697778874246 1103 100 1.1450820248169897 1104 1 0.28384440503607067 1104 2 0.18361219683513497 1104 3 0.2280656399158616 1104 4 0.1492163999698164 1104 5 0.1840296908672059 1104 6 0.19449625111646895 1104 7 0.17137121844687897 1104 8 0.16353470815236193 1104 9 0.2136765725215639 1104 10-11 0.2216207865773896 1104 12-13 0.29990509592586534 1104 14-15 0.35565981499514265 1104 16-17 0.3347980567861626 1104 18-19 0.27519757572829207 1104 20-21 0.3142333174981502 1104 22-23 0.2947497024189687 1104 24-25 0.30979030746561875 1104 26-27 0.33866334726653946 1104 28-29 0.3683834904836303 1104 30-31 0.31989317308833165 1104 32-33 0.4275253293657997 1104 34-35 0.44330089588282107 1104 36-37 0.4274571532290352 1104 38-39 0.4503915209199647 1104 40-41 0.42354622597108715 1104 42-43 0.3865244555560423 1104 44-45 0.4050089917927622 1104 46-47 0.4025496677249265 1104 48-49 0.4479265069423164 1104 50-51 0.4214239063477905 1104 52-53 0.4279211800390854 1104 54-55 0.4318161160767957 1104 56-57 0.39373683734889653 1104 58-59 0.4624002123812758 1104 60-61 0.4757574388624448 1104 62-63 0.4820492849479834 1104 64-65 0.4452461601639186 1104 66-67 0.5003064344385564 1104 68-69 0.46317469237854425 1104 70-71 0.4008535075771107 1104 72-73 0.424455965871374 1104 74-75 0.3664484267612309 1104 76-77 0.33146032919287194 1104 78-79 0.4055840526575736 1104 80-81 0.3847449193978498 1104 82-83 0.3819579162531319 1104 84-85 0.4201583937849378 1104 86-87 0.4436031817308681 1104 88-89 0.41197477477838973 1104 90-91 0.415373307058573 1104 92-93 0.49623200024350567 1104 94-95 0.5091967699058166 1104 96-97 0.5008484481083926 1104 98-99 0.4930872215278086 1104 100 0.535003555148343 1105 1 0.2496767821205026 1105 2 0.16994359948969162 1105 3 0.2187262383353783 1105 4 0.1769524920550083 1105 5 0.23799527429790857 1105 6 0.20785061842932606 1105 7 0.21230839132059742 1105 8 0.2069297518810842 1105 9 0.22250499105020793 1105 10-11 0.26656502695214357 1105 12-13 0.28410369291467674 1105 14-15 0.32581515736720945 1105 16-17 0.3678599273139085 1105 18-19 0.34817913183720606 1105 20-21 0.3454234540442016 1105 22-23 0.37486093846595736 1105 24-25 0.3813408047500104 1105 26-27 0.39495032892543946 1105 28-29 0.34314931264442095 1105 30-31 0.39737309186136827 1105 32-33 0.36615190770093875 1105 34-35 0.39413675532157555 1105 36-37 0.32127239754975534 1105 38-39 0.32167576494489225 1105 40-41 0.32961363050528547 1105 42-43 0.3403070370714474 1105 44-45 0.35778409253978083 1105 46-47 0.45018619804134374 1105 48-49 0.39596105357544786 1105 50-51 0.2754355260830934 1105 52-53 0.25809502504075255 1105 54-55 0.3019744268412623 1105 56-57 0.4032800510584664 1105 58-59 0.39248006528327295 1105 60-61 0.45280354229676334 1105 62-63 0.3602811300293638 1105 64-65 0.34937236194327426 1105 66-67 0.3552747680900552 1105 68-69 0.3621691206116253 1105 70-71 0.34490047530658785 1105 72-73 0.33666377851231744 1105 74-75 0.35818723340455705 1105 76-77 0.34061017322115816 1105 78-79 0.38216478978387514 1105 80-81 0.39585175215463764 1105 82-83 0.4208372904362996 1105 84-85 0.4230148054076075 1105 86-87 0.4527676789187609 1105 88-89 0.5119750474928821 1105 90-91 0.5588480987990998 1105 92-93 0.5454158286934998 1105 94-95 0.5417762714256789 1105 96-97 0.6166702837286806 1105 98-99 0.6040564593327531 1105 100 0.6689289027019889 1106 1 0.19504728837615204 1106 2 0.08187643499353214 1106 3 0.14399676057391275 1106 4 0.13673217581194308 1106 5 0.193221044483451 1106 6 0.15146935011107843 1106 7 0.1385923166091274 1106 8 0.16610852151710276 1106 9 0.1812983119199032 1106 10-11 0.19164274699408423 1106 12-13 0.1636069110442051 1106 14-15 0.2981457563889123 1106 16-17 0.2505157755291876 1106 18-19 0.22718033670016524 1106 20-21 0.2455310427666717 1106 22-23 0.28843036523161913 1106 24-25 0.26985302698140146 1106 26-27 0.2804734654224674 1106 28-29 0.313332786568445 1106 30-31 0.23841679467822985 1106 32-33 0.28509831946745123 1106 34-35 0.2740908243952944 1106 36-37 0.26171317087818124 1106 38-39 0.2484295081982708 1106 40-41 0.3085548879109794 1106 42-43 0.324582170844387 1106 44-45 0.3586928365425095 1106 46-47 0.32210122672258734 1106 48-49 0.3399703308024016 1106 50-51 0.3312227473145768 1106 52-53 0.321575367114761 1106 54-55 0.30456170353814827 1106 56-57 0.37885354373084823 1106 58-59 0.30770435001065977 1106 60-61 0.3452118629166776 1106 62-63 0.31590627758306056 1106 64-65 0.38655318881044565 1106 66-67 0.3312690562739178 1106 68-69 0.31613052002058595 1106 70-71 0.3042019761562358 1106 72-73 0.29612193722585545 1106 74-75 0.29147028111336226 1106 76-77 0.23438999424096352 1106 78-79 0.2962884426196233 1106 80-81 0.2752870002634964 1106 82-83 0.2506169368427855 1106 84-85 0.22796286728546278 1106 86-87 0.24981773722435818 1106 88-89 0.2558277874667425 1106 90-91 0.27230398022514635 1106 92-93 0.3327262732617875 1106 94-95 0.3535926513680572 1106 96-97 0.3857207009097614 1106 98-99 0.34294371111378297 1106 100 0.39702827728442003 1107 1 0.13500033562252156 1107 2 0.06991871880924805 1107 3 0.06025864838182571 1107 4 0.08361988036580925 1107 5 0.13701637956236112 1107 6 0.11751740065072624 1107 7 0.06803550838996131 1107 8 0.07978382183941335 1107 9 0.11122032291203254 1107 10-11 0.12135258703970919 1107 12-13 0.17171958058508352 1107 14-15 0.21207981477697047 1107 16-17 0.165239938952908 1107 18-19 0.1737681401224691 1107 20-21 0.21929076049397622 1107 22-23 0.18631471043669734 1107 24-25 0.20971825208233952 1107 26-27 0.20484044523746547 1107 28-29 0.22731334011537996 1107 30-31 0.2507573418482636 1107 32-33 0.24055441233064556 1107 34-35 0.2751466580921189 1107 36-37 0.3557370863105547 1107 38-39 0.3214639124823577 1107 40-41 0.3276501995289678 1107 42-43 0.4315650300950793 1107 44-45 0.33801220185710434 1107 46-47 0.30432972129541724 1107 48-49 0.32985093903265295 1107 50-51 0.35287889751689505 1107 52-53 0.43704914726608024 1107 54-55 0.43012121796684255 1107 56-57 0.4216842109371157 1107 58-59 0.35585720475824445 1107 60-61 0.3105377671279683 1107 62-63 0.3394651980213794 1107 64-65 0.3274378496970485 1107 66-67 0.33617515996268565 1107 68-69 0.31090783359063323 1107 70-71 0.35702915769695664 1107 72-73 0.3366923107018991 1107 74-75 0.28777494593881414 1107 76-77 0.19875557478709283 1107 78-79 0.38653462639335956 1107 80-81 0.358145088578258 1107 82-83 0.34089624473880775 1107 84-85 0.31637427631462955 1107 86-87 0.3731184292886134 1107 88-89 0.350743387350299 1107 90-91 0.3391951380745155 1107 92-93 0.3124376288141697 1107 94-95 0.30209556610689603 1107 96-97 0.40888117549290826 1107 98-99 0.42439944069676017 1107 100 0.4273692743981172 1108 1 0.12588287806480736 1108 2 0.0925381157492211 1108 3 0.09388137242039107 1108 4 0.09500687695344823 1108 5 0.08290699193518947 1108 6 0.07928087468767586 1108 7 0.0925427143765063 1108 8 0.08625182646643736 1108 9 0.16261066461802898 1108 10-11 0.15592844588544352 1108 12-13 0.12412811340863072 1108 14-15 0.15587935790701835 1108 16-17 0.09300289087492075 1108 18-19 0.15714506884185653 1108 20-21 0.20584025327620736 1108 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-0.9638288882135626 2316 82-83 -0.9613973268788634 2316 84-85 -0.9820172943902534 2316 86-87 -0.9763337400438843 2316 88-89 -0.9162490390190854 2316 90-91 -1.0220809013313037 2316 92-93 -1.044643278865859 2316 94-95 -1.0287889294828396 2316 96-97 -1.0625382555830178 2316 98-99 -1.1153027715791453 2316 100 -1.1832456509733085 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 32.0 3 42.0 4 63.0 5 131.0 6 366.0 7 901.0 8 2008.0 9 3857.0 10 6767.0 11 13317.0 12 22350.0 13 31974.0 14 35714.0 15 44703.0 16 60048.0 17 79043.0 18 77863.0 19 49305.0 20 34024.0 21 30149.0 22 32058.0 23 35600.0 24 39242.0 25 41574.0 26 48528.0 27 59447.0 28 73352.0 29 91961.0 30 112940.0 31 140387.0 32 175441.0 33 227113.0 34 319426.0 35 506019.0 36 982385.0 37 2079300.0 38 1764817.0 39 242869.0 40 290.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.21642814871689 17.171792130260567 13.612441171987163 22.99933854903538 2 13.094635257849413 23.854076332808365 34.91803102905204 28.133257380290182 3 21.232982707468377 29.17991277831573 28.955259796629228 20.63184471758667 4 14.22005259824394 19.79617108789844 32.099436722962224 33.8843395908954 5 12.4431276745029 38.37298333138211 32.85605632165217 16.327832672462826 6 31.06665598629197 30.91664405124115 18.840984669822376 19.175715292644497 7 23.29835778522963 33.70628469503199 22.467271036565194 20.528086483173187 8 29.504276123763397 30.26207817766375 22.146873190821772 18.086772507751085 9 29.49957443707683 14.994428970105577 18.901852089491182 36.60414450332641 10-11 24.769362721525244 27.40256318413931 24.033153204609743 23.7949208897257 12-13 28.531558887790638 24.94941659467894 25.03722175108531 21.481802766445117 14-15 28.186932760752487 22.480578515800836 25.041613758550383 24.290874964896293 16-17 23.412964322945705 26.110143867569903 29.415169839147342 21.061721970337047 18-19 25.87472134804189 25.43151705345965 29.634999087792412 19.05876251070605 20-21 23.75112304320563 25.602089801394655 29.266408821617407 21.380378333782303 22-23 21.878075180073438 27.66613842446293 24.37634376913092 26.079442626332717 24-25 22.785949481481907 28.912786072047545 23.27155891914946 25.029705527321084 26-27 24.759889679956274 25.070671308275994 24.69620228493683 25.473236726830905 28-29 23.57490272471273 23.95070184288051 29.097877830424025 23.376517601982737 30-31 26.483330419832168 24.647436219440888 27.17098367166784 21.6982496890591 32-33 20.553676829158434 26.6363834918342 28.874629009117704 23.935310669889663 34-35 19.858541594555295 28.001927316020886 29.162208263722416 22.977322825701403 36-37 24.274051894269085 27.37940940371389 26.32707006252108 22.019468639495944 38-39 25.36798567032189 24.95479444154985 25.761197170849048 23.91602271727921 40-41 22.82984315776276 27.068694563781186 25.96595786177608 24.13550441667997 42-43 26.922192563888565 25.009833955676104 25.71891691394162 22.349056566493715 44-45 26.756537854660877 23.978484173998446 27.134703036553287 22.130274934787387 46-47 23.896199055624393 25.034178359733435 30.313747027895914 20.755875556746258 48-49 24.687666015753194 26.677839088724713 28.746474069863048 19.888020825659048 50-51 21.858047349341845 27.988308004068973 26.558803302435624 23.59484134415356 52-53 20.87410059726979 28.966623663098872 23.205884833181276 26.95339090645006 54-55 22.78276173642493 27.706889955992743 22.645636353612943 26.864711953969383 56-57 24.717317168512363 24.496979144929025 23.851969559990525 26.933734126568083 58-59 23.664589099545687 24.425102738344854 27.591450894146174 24.31885726796328 60-61 24.372365126675906 25.618708067039275 25.864016033816956 24.144910772467867 62-63 19.931042982426174 26.717324454321922 27.585881460910848 25.765751102341056 64-65 19.762775745482763 27.23680328310813 27.13968179651977 25.860739174889343 66-67 23.745368129785998 26.47519616170446 24.802847055555116 24.976588652954423 68-69 24.473094165147582 24.858369809295787 24.71130280084356 25.95723322471307 70-71 22.353583197880514 26.198451882932062 25.32376902135675 26.124195897830678 72-73 24.896054682547426 24.52682853776253 25.437998934677285 25.13911784501276 74-75 25.177007477351506 23.542093281997833 26.295566000284392 24.985333240366273 76-77 22.38574491972854 25.584661457114155 28.59833487479345 23.431258748363852 78-79 22.771523444495898 25.671509017773264 27.932720302551278 23.624247235179556 80-81 20.662024275705836 26.462110701012108 26.854929256359267 26.020935766922793 82-83 20.35278656323176 27.476123888809934 23.918956045612056 28.252133502346254 84-85 21.392197930614337 26.719116607754916 23.69646706326945 28.192218398361295 86-87 22.202666716675417 23.93426438912524 25.07033150444185 28.792737389757495 88-89 22.04418235833429 23.314911236175472 27.189720096061826 27.45118630942841 90-91 22.89332329586249 24.425868276948464 26.01896626552504 26.661842161664005 92-93 19.94039123443056 25.42836935198574 26.94724088437976 27.68399852920394 94-95 19.148169786560885 26.106492439912422 27.443493324204866 27.301844449321834 96-97 21.758381288490515 25.118605587420735 25.55386054949943 27.569152574589328 98-99 22.53513740578878 24.025290787935713 25.206170970473675 28.23340083580183 100 21.799969700050596 25.29818056974889 26.247940654963813 26.653909075236704 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 930.0 1 560.5 2 222.0 3 278.0 4 253.0 5 295.0 6 494.5 7 895.5 8 1336.0 9 1407.5 10 1162.5 11 1037.5 12 1164.0 13 1396.0 14 1737.0 15 2208.0 16 2689.5 17 3147.0 18 3785.0 19 4600.0 20 5598.5 21 6667.5 22 8016.0 23 9844.0 24 12100.0 25 14885.5 26 18763.0 27 23928.0 28 29795.0 29 37538.5 30 46066.5 31 53912.5 32 62648.5 33 71719.0 34 80472.0 35 87579.5 36 94522.5 37 102319.5 38 104932.0 39 106010.0 40 104423.5 41 101618.0 42 102258.0 43 110068.0 44 126903.5 45 144491.5 46 175187.5 47 235972.0 48 453590.5 49 749584.0 50 1096866.0 51 1010337.0 52 506052.0 53 278488.0 54 211128.0 55 167406.0 56 143750.0 57 124556.5 58 112699.0 59 103554.0 60 86162.5 61 67654.0 62 53272.5 63 41593.0 64 30468.5 65 24074.5 66 19254.5 67 14160.5 68 10561.5 69 8443.5 70 7047.5 71 6221.5 72 5958.5 73 6472.5 74 5044.5 75 3657.5 76 2847.0 77 2093.5 78 1521.5 79 918.5 80 648.5 81 517.5 82 448.0 83 367.0 84 243.0 85 144.5 86 97.5 87 52.0 88 29.5 89 16.0 90 7.5 91 5.5 92 5.0 93 5.0 94 4.5 95 2.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.06236767297049886 3 0.025383750059943158 4 0.006938671520343301 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 5.358047506056603E-5 12-13 1.7413654394683958E-4 14-15 3.5497064727624993E-4 16-17 4.487364786322405E-4 18-19 0.0 20-21 0.00266562863426316 22-23 0.0053044670309960375 24-25 5.960827850487971E-4 26-27 4.7552671616252353E-4 28-29 0.005096842690136344 30-31 0.0021566141211877827 32-33 0.0012658387233058725 34-35 0.0013663021140444338 36-37 0.004333320920523277 38-39 0.004547642820765542 40-41 9.376583135599054E-4 42-43 0.002685721312410872 44-45 2.3441457838997636E-4 46-47 0.002089638527362075 48-49 0.0 50-51 0.001714575201938113 52-53 0.006985554436021297 54-55 0.014326079519318843 56-57 0.025095755006492613 58-59 0.027178695974472117 60-61 0.02585927677610568 62-63 0.008184417565501461 64-65 0.007079320267377286 66-67 1.2055606888627357E-4 68-69 0.001359604554661863 70-71 1.9422922209455184E-4 72-73 8.371949228213442E-4 74-75 0.005311164590378607 76-77 0.0010917021793590328 78-79 0.0012457460451581602 80-81 0.0 82-83 0.0013261167577490094 84-85 2.6790237530283016E-5 86-87 6.161754631965093E-4 88-89 7.367315320827828E-5 90-91 6.161754631965093E-4 92-93 8.17102244673632E-4 94-95 0.001547136217373844 96-97 1.5404386579912733E-4 98-99 0.0 100 6.295705819616508E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7465406.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.7113120850609 #Duplication Level Percentage of deduplicated Percentage of total 1 78.66183048096698 19.438370421973307 2 11.970293427094308 5.916033132533612 3 3.55024020587185 2.6319328111269042 4 1.5401759090711409 1.5223907021979737 5 0.8829182761093062 1.0909034533270514 6 0.5359560001269609 0.7946505589798961 7 0.3672555496067656 0.6352756550912344 8 0.2789996907190672 0.5515558743195471 9 0.21788543617530434 0.48458115109058075 >10 1.521192600392238 7.431406344084033 >50 0.21421218013195972 3.742437728151608 >100 0.21064804998913925 11.104599008795384 >500 0.028607842808378192 4.781767442294579 >1k 0.015345202942469768 6.452252197451316 >5k 0.0014797159980238703 2.7522207751004317 >10k+ 0.0029594319960477406 30.669622743482734 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 240693 3.2241113209382046 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 213106 2.854580179564246 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 108109 1.4481328945806833 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 98427 1.3184413546965832 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 94233 1.2622622265955796 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 86746 1.161972972400965 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 86062 1.1528107111656085 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 85352 1.143300176842358 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 81228 1.0880587070549144 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 79374 1.063224156864342 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 68107 0.9123013537374927 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 59637 0.7988446977967442 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 48402 0.6483505384703793 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 42220 0.5655419142642744 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 37291 0.499517373870892 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 36972 0.49524433098481185 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 36897 0.4942396970774262 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 34378 0.46049739290803476 No Hit CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC 31980 0.4283758981092254 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 30785 0.4123687311848813 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 30658 0.41066755110170833 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 30569 0.4094753855316107 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC 28494 0.3816805140939421 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 27361 0.3665038445330368 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 26722 0.35794436364211135 No Hit GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG 23361 0.31292336947247074 No Hit GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA 22744 0.30465858119437844 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 22537 0.3018857916099941 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 22422 0.3003453529520029 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 22149 0.29668848552911925 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 21525 0.28832993141967095 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 21198 0.28394972758346965 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 21055 0.2820342256000545 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 21045 0.281900274412403 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 20968 0.2808688502674871 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 20530 0.27500178824835514 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20221 0.27086269654992645 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 18694 0.25040835019555535 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 18312 0.24529141482727126 No Hit TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA 17715 0.23729452892448183 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT 16869 0.2259622584491721 No Hit GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG 16652 0.2230555176771364 No Hit CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 16611 0.22250631780776556 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16579 0.22207767400728107 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 15761 0.2111204668573953 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 15660 0.20976755986211604 No Hit ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG 14923 0.19989535733220673 No Hit ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC 14577 0.19526064623946776 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT 14261 0.19102778870968304 No Hit TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG 13991 0.18741110664309482 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13505 0.18090107892323606 No Hit CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA 13269 0.17773983089466266 No Hit TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG 12512 0.16759972598945055 No Hit GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA 11185 0.14982440338810776 No Hit TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA 9983 0.13372347063240766 No Hit ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACT 9975 0.13361630968228652 No Hit GAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAG 9694 0.12985228130928178 No Hit GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA 9484 0.12703930636860206 No Hit TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC 9366 0.12545868235431537 No Hit CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT 8807 0.11797081096460126 No Hit TGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC 8506 0.11393888021629367 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA 8434 0.11297443166520346 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 8285 0.1109785589691974 No Hit GATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC 8079 0.10821916450357823 No Hit GTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACT 8038 0.10766996463420744 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT 7981 0.10690644286459436 No Hit CATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 7848 0.10512489206883054 No Hit CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTG 7741 0.10369161436096042 No Hit CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC 7597 0.10176271725878003 No Hit TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA 7560 0.10126709786446979 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.006416261888502782 0.0 0.0 4.018535629542452E-5 1.3395118765141508E-5 2 0.009845412292379008 0.0 0.0 8.037071259084904E-5 1.3395118765141508E-5 3 0.010300846330393819 0.0 0.0 9.376583135599055E-5 1.3395118765141508E-5 4 0.01226992878886962 0.0 0.0 1.0716095012113206E-4 1.3395118765141508E-5 5 0.05287053376601353 1.3395118765141508E-5 0.0 3.616682066588207E-4 1.3395118765141508E-5 6 0.11351023641580914 1.3395118765141508E-5 0.0 8.171022446736319E-4 1.3395118765141508E-5 7 0.12883425228313103 1.3395118765141508E-5 0.0 9.644485510901885E-4 1.3395118765141508E-5 8 0.14853847198665418 1.3395118765141508E-5 0.0 0.0010716095012113206 1.3395118765141508E-5 9 0.16299180513424186 1.3395118765141508E-5 0.0 0.0011519802138021697 1.3395118765141508E-5 10-11 0.2932861253627733 1.3395118765141508E-5 0.0 0.0022436823931612027 1.3395118765141508E-5 12-13 0.49985894939940306 1.3395118765141508E-5 0.0 0.004936101264954646 2.6790237530283016E-5 14-15 0.7259752517143743 1.3395118765141508E-5 0.0 0.008244695599944597 3.348779691285377E-5 16-17 0.8186694735691535 1.3395118765141508E-5 0.0 0.009791831817318443 5.358047506056603E-5 18-19 0.8607033562541675 2.6790237530283016E-5 0.0 0.010863441318529763 5.358047506056603E-5 20-21 1.0032876979497163 2.6790237530283016E-5 0.0 0.014433240469439973 5.358047506056603E-5 22-23 1.0461788682356994 2.6790237530283016E-5 0.0 0.017855693313933628 5.358047506056603E-5 24-25 1.2364833205320647 2.6790237530283016E-5 0.0 0.03246976788670301 5.358047506056603E-5 26-27 1.6244595404456235 2.6790237530283016E-5 0.0 0.0810940490041667 6.697559382570753E-5 28-29 1.7224649268907815 2.6790237530283016E-5 0.0 0.1249898531975354 6.697559382570753E-5 30-31 1.759261318138625 2.6790237530283016E-5 0.0 0.21822657736230286 6.697559382570753E-5 32-33 1.792869671120365 2.6790237530283016E-5 0.0 0.3665708201268625 8.037071259084904E-5 34-35 1.8190571283062167 2.6790237530283016E-5 0.0 0.5663054360338875 8.037071259084904E-5 36-37 1.8554717586692537 2.6790237530283016E-5 0.0 0.8548228991162704 8.037071259084904E-5 38-39 1.8935674764373163 2.6790237530283016E-5 0.0 1.2922338048325839 9.376583135599055E-5 40-41 1.928803336349021 2.6790237530283016E-5 0.0 1.9569464808745836 9.376583135599055E-5 42-43 2.0019875677223715 2.6790237530283016E-5 0.0 2.420591726692426 9.376583135599055E-5 44-45 2.0856869673263585 2.6790237530283016E-5 0.0 2.862958826351842 1.0046339073856132E-4 46-47 2.120581251709552 3.348779691285377E-5 0.0 3.3763401481446556 1.0716095012113206E-4 48-49 2.145375616543829 4.018535629542452E-5 0.0 3.828043645583375 1.0716095012113206E-4 50-51 2.1842281585221217 4.018535629542452E-5 0.0 4.338156558397493 1.2055606888627356E-4 52-53 2.1973888627088733 4.018535629542452E-5 0.0 4.985522555638635 1.2055606888627356E-4 54-55 2.238210487145642 4.018535629542452E-5 0.0 5.532217537800355 1.2055606888627356E-4 56-57 2.3138058398967183 4.688291567799528E-5 0.0 6.091289609701066 1.272536282688443E-4 58-59 2.339537863044555 6.0278034443136787E-5 0.0 6.673026222552397 1.3395118765141507E-4 60-61 2.3596171460735023 6.697559382570753E-5 0.0 7.167373080579944 1.3395118765141507E-4 62-63 2.376367742089312 6.697559382570753E-5 0.0 7.681812616755204 1.3395118765141507E-4 64-65 2.38923375366323 6.697559382570753E-5 0.0 8.267855760289528 1.473463064165566E-4 66-67 2.405944164322744 6.697559382570753E-5 0.0 8.861078687481967 1.5404386579912735E-4 68-69 2.426967803224634 7.36731532082783E-5 0.0 9.62462054977318 1.607414251816981E-4 70-71 2.453289211598137 8.706827197341979E-5 0.0 10.581875922086489 1.6743898456426882E-4 72-73 2.5161123186066505 1.1385850950370281E-4 0.0 11.304134028343537 1.875316627119811E-4 74-75 2.576142543352632 1.3395118765141507E-4 0.0 11.97920113119099 2.009267814771226E-4 76-77 2.5953578412212277 1.4064874703398583E-4 0.0 12.704935270767592 2.1432190024226413E-4 78-79 2.609791081690668 1.473463064165566E-4 0.0 13.370049800372545 2.2101945962483486E-4 80-81 2.636541133864655 1.473463064165566E-4 0.0 14.145988845080897 2.4111213777254712E-4 82-83 2.6466946338886324 1.473463064165566E-4 0.0 15.1078186504525 2.545072565376886E-4 84-85 2.682821269198219 1.473463064165566E-4 0.0 15.971174240222165 2.545072565376886E-4 86-87 2.7405743237541267 1.473463064165566E-4 0.0 16.793721600673827 2.545072565376886E-4 88 2.7559787103340394 1.473463064165566E-4 0.0 17.464435289922612 2.545072565376886E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCGATC 3325 0.0 32.65623 3 CGATCTC 2940 0.0 31.00809 5 TATCACG 330 0.0 28.50613 2 GATCTAC 3530 0.0 28.380962 1 AGTACTC 3220 0.0 27.436113 5 CCGATCT 4205 0.0 26.26326 4 GATGTAA 2375 0.0 25.349451 1 ATCTACA 4250 0.0 24.34759 2 TCTCGCG 1455 0.0 23.577194 8 TCTACAC 5070 0.0 23.178097 3 TATTCGC 320 0.0 22.027922 9 CTCGCGC 1635 0.0 21.843796 9 TGTAAGA 2735 0.0 21.655037 3 TACACTC 5210 0.0 21.556627 5 AAGTACT 3945 0.0 21.442347 4 GTAAGAA 2880 0.0 20.23373 4 CGCCCCA 2930 0.0 20.066175 1 CTACACT 6530 0.0 19.647 4 AAAGTAC 4260 0.0 19.419981 3 GTATAAG 1690 0.0 19.203686 1 >>END_MODULE