FastQCFastQC Report
Fri 27 May 2016
ERR765550_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765550_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6800603
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA463570.6816601410198477No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT425610.6258415614027167No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG198250.29151826683604376No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC129150.18990963007250974No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC128100.1883656493402129No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG113370.16670580535284887No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT107150.1575595575862905No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG105480.1551038929930184No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC76650.11271059345766839No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG73230.10768162764390157No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG16050.020.280481
GTATAAG21400.019.1215951
GTACATA18650.018.4504991
CATGGGG146600.018.4284084
GTACAAG33600.017.9911651
GTACAAA38550.016.8872471
GTATTAT18900.016.238181
GTCCTAC19900.016.12321
GTATAAT22350.016.0202181
CTAGTAC23500.015.829523
ATATACG30950.015.4746996
GTATATG24700.015.2490681
GTATAGA15000.015.190081
TACGCTA31000.015.1497469
GTATAAA73200.014.9918811
TAGTACT24250.014.956454
GTATTAG27300.014.8187591
GTACATG799950.014.4508521
TCTAACG8400.014.3926152
TACATGG783350.014.3768562
Produced by FastQC (version 0.11.5)