##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765550_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6800603 Sequences flagged as poor quality 0 Sequence length 99 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.903778091442774 33.0 33.0 33.0 27.0 33.0 2 30.804971265048113 33.0 33.0 33.0 27.0 33.0 3 30.82072516216577 33.0 33.0 33.0 27.0 33.0 4 34.34360011898945 37.0 33.0 37.0 27.0 37.0 5 34.49132657795199 37.0 37.0 37.0 27.0 37.0 6 34.42327437728684 37.0 37.0 37.0 27.0 37.0 7 34.292246584604335 37.0 33.0 37.0 27.0 37.0 8 34.23980079413546 37.0 33.0 37.0 27.0 37.0 9 34.20500740890183 37.0 37.0 37.0 27.0 37.0 10-11 34.15025785213459 37.0 33.0 37.0 27.0 37.0 12-13 34.08636925284419 37.0 33.0 37.0 27.0 37.0 14-15 35.29926000679646 37.0 33.0 40.0 27.0 40.0 16-17 35.31786416586882 37.0 33.0 40.0 27.0 40.0 18-19 35.299481237178526 37.0 33.0 40.0 27.0 40.0 20-21 35.29977422590321 37.0 33.0 40.0 27.0 40.0 22-23 35.33554561264641 37.0 33.0 40.0 27.0 40.0 24-25 35.266372040832266 37.0 33.0 40.0 27.0 40.0 26-27 35.26236099945843 37.0 33.0 40.0 27.0 40.0 28-29 35.164466518630775 37.0 33.0 40.0 27.0 40.0 30-31 35.113828214939176 37.0 33.0 40.0 27.0 40.0 32-33 34.95154525267833 37.0 33.0 40.0 27.0 40.0 34-35 34.89719029327252 37.0 33.0 40.0 27.0 40.0 36-37 34.78778396562775 37.0 33.0 40.0 27.0 40.0 38-39 34.55136647441411 37.0 33.0 40.0 27.0 40.0 40-41 34.45640489527179 37.0 33.0 40.0 22.0 40.0 42-43 34.30753118510226 37.0 33.0 40.0 22.0 40.0 44-45 34.15329008324703 37.0 33.0 40.0 22.0 40.0 46-47 33.99884855798817 37.0 33.0 40.0 22.0 40.0 48-49 33.809143468601235 37.0 33.0 40.0 22.0 40.0 50-51 33.635982206283764 37.0 33.0 38.5 22.0 40.0 52-53 33.448370975338506 37.0 33.0 37.0 22.0 40.0 54-55 33.3122547368226 37.0 33.0 37.0 22.0 40.0 56-57 33.10739238858672 37.0 33.0 37.0 22.0 40.0 58-59 32.86136832277961 37.0 33.0 37.0 22.0 40.0 60-61 32.61407848686359 37.0 33.0 37.0 22.0 40.0 62-63 32.41964749302378 37.0 33.0 37.0 22.0 40.0 64-65 32.111148232002364 35.0 27.0 37.0 22.0 40.0 66-67 31.892877146335408 33.0 27.0 37.0 22.0 38.5 68-69 31.58025472152984 33.0 27.0 37.0 22.0 37.0 70-71 31.403108591988094 33.0 27.0 37.0 22.0 37.0 72-73 31.097606050522284 33.0 27.0 37.0 18.5 37.0 74-75 30.80540012407723 33.0 27.0 37.0 15.0 37.0 76-77 30.596130299033774 33.0 27.0 37.0 15.0 37.0 78-79 30.33981251368445 33.0 27.0 37.0 15.0 37.0 80-81 30.07116663037087 33.0 27.0 37.0 15.0 37.0 82-83 29.904571035833147 33.0 27.0 37.0 15.0 37.0 84-85 29.7055379500906 33.0 27.0 37.0 15.0 37.0 86-87 29.47329736789517 33.0 27.0 37.0 15.0 37.0 88-89 29.273383624952082 33.0 27.0 37.0 15.0 37.0 90-91 29.06149123835048 33.0 27.0 35.0 15.0 37.0 92-93 28.881110469174573 33.0 27.0 33.0 15.0 37.0 94-95 28.62571355804772 33.0 27.0 33.0 15.0 37.0 96-97 28.558413717136553 33.0 27.0 33.0 15.0 37.0 98-99 27.411384696327666 30.0 24.5 33.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.38081407224722597 1101 2 0.3199551130301579 1101 3 0.4435227338647394 1101 4 0.47499947488081773 1101 5 0.45285365227954344 1101 6 0.40725279240592727 1101 7 0.3026623133199067 1101 8 0.4073770283189617 1101 9 0.3731258008997287 1101 10-11 0.3946461794551297 1101 12-13 0.36408554017190653 1101 14-15 0.5376785393974188 1101 16-17 0.4946698723461225 1101 18-19 0.48126921289943425 1101 20-21 0.43378208187564127 1101 22-23 0.45553989896962577 1101 24-25 0.476580313080639 1101 26-27 0.5230262704578479 1101 28-29 0.5166329399753025 1101 30-31 0.5085260918642476 1101 32-33 0.6366040724895541 1101 34-35 0.4935364728597875 1101 36-37 0.5206684579407863 1101 38-39 0.48810217440851744 1101 40-41 0.3991828582580652 1101 42-43 0.4797246560771242 1101 44-45 0.46433771396475265 1101 46-47 0.5120531503339976 1101 48-49 0.5509179178092083 1101 50-51 0.4875954990122153 1101 52-53 0.5105338071540189 1101 54-55 0.6050666023127533 1101 56-57 0.6713288205951358 1101 58-59 0.5777443750447162 1101 60-61 0.6522538427222813 1101 62-63 0.644743568913924 1101 64-65 0.4651436439048311 1101 66-67 0.5456735272216449 1101 68-69 0.48261974179132494 1101 70-71 0.5219493230401753 1101 72-73 0.5629178590116553 1101 74-75 0.5417843252697772 1101 76-77 0.40870793444279485 1101 78-79 0.4660181116465978 1101 80-81 0.4002888072791677 1101 82-83 0.5055914709604217 1101 84-85 0.4076801739924072 1101 86-87 0.4505781076679014 1101 88-89 0.44684298758825847 1101 90-91 0.36223985242772727 1101 92-93 0.5659699847929396 1101 94-95 0.5638362930394223 1101 96-97 0.4535441881101967 1101 98-99 0.5121450688632798 1102 1 0.3850158398647423 1102 2 0.3763150080790503 1102 3 0.3677987518549344 1102 4 0.40928341284027425 1102 5 0.31315182700114974 1102 6 0.33234236879140866 1102 7 0.3270582278029721 1102 8 0.36751582942427063 1102 9 0.29702763678137956 1102 10-11 0.2794401556565518 1102 12-13 0.34334319013192527 1102 14-15 0.3806731162537744 1102 16-17 0.47113044095112855 1102 18-19 0.47124060468205187 1102 20-21 0.4457574669857536 1102 22-23 0.29451025047745105 1102 24-25 0.5079886232558479 1102 26-27 0.5037550800087587 1102 28-29 0.3227316429415694 1102 30-31 0.3623783314589133 1102 32-33 0.497010030954236 1102 34-35 0.46768248765465614 1102 36-37 0.5589137408691727 1102 38-39 0.47612535438455694 1102 40-41 0.43664963433816695 1102 42-43 0.37441008821993904 1102 44-45 0.42256161780951373 1102 46-47 0.48094022000805836 1102 48-49 0.42437468211773677 1102 50-51 0.49490809378733047 1102 52-53 0.47467061810932876 1102 54-55 0.5378987992194837 1102 56-57 0.43856610561577725 1102 58-59 0.531336461925271 1102 60-61 0.5431074978972248 1102 62-63 0.5455810337292633 1102 64-65 0.3668832494455003 1102 66-67 0.45169188067611543 1102 68-69 0.4738010538810826 1102 70-71 0.3918010078895904 1102 72-73 0.3603643287950504 1102 74-75 0.36961628069561314 1102 76-77 0.357223045935978 1102 78-79 0.44876263531675065 1102 80-81 0.4725786109021435 1102 82-83 0.36043019023032485 1102 84-85 0.2793891613034667 1102 86-87 0.4224213736511615 1102 88-89 0.48952253193967366 1102 90-91 0.43536527624652877 1102 92-93 0.5084558242700332 1102 94-95 0.4594849252682067 1102 96-97 0.41009898505364717 1102 98-99 0.43732833945940897 1103 1 0.2777751946328948 1103 2 0.31904825519651325 1103 3 0.3574906128956492 1103 4 0.37854111236045185 1103 5 0.35051934235664817 1103 6 0.2848419441980994 1103 7 0.2280799330946266 1103 8 0.3016645464990759 1103 9 0.34553227246602347 1103 10-11 0.24091965100869572 1103 12-13 0.2541886838402263 1103 14-15 0.30388074196910964 1103 16-17 0.30831418135186084 1103 18-19 0.3634245460679324 1103 20-21 0.2838809510177924 1103 22-23 0.279810667216978 1103 24-25 0.395440610381776 1103 26-27 0.4684409358582329 1103 28-29 0.40798565907359574 1103 30-31 0.45275159851654934 1103 32-33 0.5170817370352623 1103 34-35 0.44553427688978786 1103 36-37 0.5135842946532847 1103 38-39 0.4592924652805337 1103 40-41 0.4007376069348325 1103 42-43 0.3596435640502378 1103 44-45 0.37550369930972494 1103 46-47 0.32560897035534 1103 48-49 0.37615305913949015 1103 50-51 0.4333792445386351 1103 52-53 0.44038546594825334 1103 54-55 0.47590787439274607 1103 56-57 0.3809758042318805 1103 58-59 0.4172956087677733 1103 60-61 0.4346731883595467 1103 62-63 0.3568531199251339 1103 64-65 0.3415000966193489 1103 66-67 0.45722610491089455 1103 68-69 0.39117266207570367 1103 70-71 0.3666572691672805 1103 72-73 0.45744380806837626 1103 74-75 0.4039244317576305 1103 76-77 0.28752797191724255 1103 78-79 0.27408457189957147 1103 80-81 0.32209096412880456 1103 82-83 0.3345875095252069 1103 84-85 0.30224411956997344 1103 86-87 0.3914737258414789 1103 88-89 0.4006304432933696 1103 90-91 0.40136689105152357 1103 92-93 0.47218202359798056 1103 94-95 0.37247103843343865 1103 96-97 0.2873995422368303 1103 98-99 0.3371846696502665 1104 1 0.12602883551774724 1104 2 0.02965019841542116 1104 3 0.009863125021304597 1104 4 0.06411668490093092 1104 5 0.03955034124021495 1104 6 0.09261755754668144 1104 7 -0.1639079960410399 1104 8 -0.04302359404687195 1104 9 -0.033528222578354416 1104 10-11 0.009234251219055523 1104 12-13 -0.05206437361629668 1104 14-15 0.031511629097494165 1104 16-17 0.11666090607912594 1104 18-19 0.06696807887359313 1104 20-21 0.09172336223829802 1104 22-23 0.08679930293186544 1104 24-25 0.09089678604730267 1104 26-27 0.088254663817672 1104 28-29 0.05286986793989712 1104 30-31 0.09180703851671268 1104 32-33 0.04614583633804159 1104 34-35 0.04886012601933487 1104 36-37 0.09005529547049207 1104 38-39 -0.011286040156868182 1104 40-41 -0.012975348814322274 1104 42-43 -0.12084084104935044 1104 44-45 -0.11234865703189456 1104 46-47 -0.0807596207746144 1104 48-49 -0.08645519280130287 1104 50-51 0.00902205369003184 1104 52-53 -0.11766485973064533 1104 54-55 0.0707652918212176 1104 56-57 -0.11147509555560475 1104 58-59 -0.042170321138677025 1104 60-61 0.03085694785480797 1104 62-63 -0.10545585994054818 1104 64-65 -0.039992035587630426 1104 66-67 -0.05770810592760256 1104 68-69 -0.09623402757616617 1104 70-71 -0.12122615293454686 1104 72-73 0.060562155157885655 1104 74-75 -0.12392080448227816 1104 76-77 -0.2609987056745844 1104 78-79 -0.14158263518033465 1104 80-81 -0.13821578022549375 1104 82-83 -0.05562059344080694 1104 84-85 -0.07848533061500618 1104 86-87 -0.05724426196769272 1104 88-89 0.05242014804112216 1104 90-91 0.03861565134974754 1104 92-93 0.06645451131588231 1104 94-95 -0.029356821963116175 1104 96-97 -0.31628753903342144 1104 98-99 -0.10902528628825081 1105 1 0.10935850177967765 1105 2 0.11228491234780691 1105 3 0.11319759268244312 1105 4 0.023077769733205855 1105 5 0.12364169538191305 1105 6 0.07727089130079179 1105 7 -0.0050089921162026485 1105 8 -0.016680375583725038 1105 9 0.0461019387418915 1105 10-11 0.07536247263277573 1105 12-13 0.031449986885426995 1105 14-15 0.08690145074172761 1105 16-17 0.06915449256617734 1105 18-19 0.1011463659635723 1105 20-21 0.12547881429441787 1105 22-23 0.028113930743607796 1105 24-25 0.135287453214481 1105 26-27 0.17399055879715775 1105 28-29 0.18650231770396175 1105 30-31 0.2068057173411333 1105 32-33 0.11245307575491381 1105 34-35 0.04574133144345183 1105 36-37 0.0673123907938944 1105 38-39 -0.0053929102036534005 1105 40-41 -0.017083494060365467 1105 42-43 0.034476878688408874 1105 44-45 0.05385247639109991 1105 46-47 -0.00797017978489123 1105 48-49 0.015468785682074326 1105 50-51 0.0057020299500649685 1105 52-53 -0.11720949644929135 1105 54-55 0.07426347380712173 1105 56-57 0.16291891997511954 1105 58-59 -0.030219420024842236 1105 60-61 0.11683797938544416 1105 62-63 0.04251487120214392 1105 64-65 -0.02335684614973843 1105 66-67 0.03792069806128495 1105 68-69 -0.03394843355399502 1105 70-71 -0.15017677594680734 1105 72-73 0.011937197511272757 1105 74-75 0.024245863949879265 1105 76-77 -0.06408524234375079 1105 78-79 -0.03216361974891413 1105 80-81 -0.05958887741267205 1105 82-83 0.042694303571494174 1105 84-85 0.05226196244630543 1105 86-87 0.05671471291923069 1105 88-89 -0.0033486610191424404 1105 90-91 0.06718549386944517 1105 92-93 0.09154484743148217 1105 94-95 -0.0025940787612483973 1105 96-97 -0.1602371636238864 1105 98-99 -0.12521629388619004 1106 1 -0.07286925418813084 1106 2 0.061392095367693855 1106 3 -0.03160923983282515 1106 4 -0.10107595839732397 1106 5 -0.08374430927265308 1106 6 -0.09720198552414416 1106 7 -0.11676701951326152 1106 8 -0.19406369933803091 1106 9 -0.11781783322575734 1106 10-11 -0.18814054235194533 1106 12-13 -0.14330369999270687 1106 14-15 -0.1288486146124299 1106 16-17 -0.11372506449080788 1106 18-19 -0.05618688461969157 1106 20-21 -0.057863244950816295 1106 22-23 -0.08193563146198102 1106 24-25 -0.05152137944283197 1106 26-27 -0.012888506809545675 1106 28-29 -0.1626548517328672 1106 30-31 -0.11248374507236036 1106 32-33 0.012461727718402926 1106 34-35 -0.22045979483766587 1106 36-37 -0.09804139858034233 1106 38-39 -0.21220925511720168 1106 40-41 -0.0832053945444926 1106 42-43 -0.1448768860123053 1106 44-45 -0.12199080868422385 1106 46-47 -0.27274232075826177 1106 48-49 -0.24614650686268646 1106 50-51 -0.21734477260924479 1106 52-53 -0.23869823224934805 1106 54-55 -0.24483914713720623 1106 56-57 -0.13115115555433476 1106 58-59 -0.09938688109578919 1106 60-61 -0.0713864376855824 1106 62-63 -0.15665354470505122 1106 64-65 -0.24462007628608617 1106 66-67 -0.1386082826255901 1106 68-69 -0.15855317481555886 1106 70-71 -0.24400376683358616 1106 72-73 -0.08618159652657909 1106 74-75 -0.21768828582435518 1106 76-77 -0.22235517123171888 1106 78-79 -0.14795262885867544 1106 80-81 -0.1487510811126782 1106 82-83 -0.16446228517194683 1106 84-85 -0.3866160683516533 1106 86-87 -0.2483266637389221 1106 88-89 -0.16182983435020049 1106 90-91 -0.14607430931741305 1106 92-93 -0.23189436955806286 1106 94-95 -0.2959020663686509 1106 96-97 -0.4307818163587456 1106 98-99 -0.025516987944282477 1107 1 0.04143168188834068 1107 2 0.12413881728927123 1107 3 0.027178950009787428 1107 4 -0.027520792456108722 1107 5 -0.004113105959675067 1107 6 0.007792261388722466 1107 7 -0.04570275957770065 1107 8 -0.13001865132537915 1107 9 -0.12474113089091077 1107 10-11 0.030063828155206807 1107 12-13 -0.08506909492071912 1107 14-15 -0.0442652365108529 1107 16-17 -0.04936850734016218 1107 18-19 -0.09553109351698197 1107 20-21 -0.1572277104300639 1107 22-23 -0.09980080254055679 1107 24-25 0.06938441330442657 1107 26-27 0.01811176289626104 1107 28-29 0.06653690902019349 1107 30-31 0.015167124129384035 1107 32-33 -0.1692834123959841 1107 34-35 -0.1196924683973748 1107 36-37 -0.09662497452275431 1107 38-39 -0.07403763161914156 1107 40-41 -0.21713569451633674 1107 42-43 -0.22599625455980288 1107 44-45 -0.14484520992143501 1107 46-47 -0.27482230207336755 1107 48-49 -0.1897092773332929 1107 50-51 -0.14401670453954551 1107 52-53 -0.23874466861381194 1107 54-55 -0.19083232901985525 1107 56-57 -0.15318644832306205 1107 58-59 -0.06667051716851802 1107 60-61 -0.03273123232474262 1107 62-63 -0.27788775183189074 1107 64-65 -0.266417608149105 1107 66-67 -0.08520358995686905 1107 68-69 -0.13666803100748126 1107 70-71 -0.09220806590018427 1107 72-73 -0.08348280556258203 1107 74-75 -0.14066759840630283 1107 76-77 -0.2259567038651653 1107 78-79 -0.01767608032616863 1107 80-81 -0.17844206991865263 1107 82-83 -0.09034637272073098 1107 84-85 -0.23012098434639583 1107 86-87 -0.2569951623490496 1107 88-89 -0.11872293600014316 1107 90-91 -0.05315096192679647 1107 92-93 -0.07250361748913647 1107 94-95 -0.05452328865722933 1107 96-97 -0.17114466155193142 1107 98-99 0.09175520349208455 1108 1 0.019311101439104306 1108 2 0.0032951183911933413 1108 3 0.010024779953234031 1108 4 0.021168150764246718 1108 5 0.038699457622549005 1108 6 -0.16975228360252004 1108 7 -0.1120595157989257 1108 8 -0.14835898412612636 1108 9 -0.07874985825564096 1108 10-11 -0.11000346300612307 1108 12-13 -0.18872001089660984 1108 14-15 -0.09673168993305126 1108 16-17 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2216 7 0.022540803233503937 2216 8 -0.02941275348455008 2216 9 0.04347826022431178 2216 10-11 -0.06833082135528912 2216 12-13 -0.01135678744940094 2216 14-15 -0.07764684582761561 2216 16-17 -0.1455742902109023 2216 18-19 -0.1223203213616415 2216 20-21 -0.11174851256230056 2216 22-23 -0.26835458114657484 2216 24-25 -0.07163870697753083 2216 26-27 -0.20601171331859547 2216 28-29 -0.08822495747486414 2216 30-31 -0.19327714111631877 2216 32-33 -0.20714694090089836 2216 34-35 -0.26152812484922805 2216 36-37 -0.23264211555085978 2216 38-39 -0.1553555854827806 2216 40-41 -0.1711084476846949 2216 42-43 -0.1602600415884794 2216 44-45 -0.08268269371534842 2216 46-47 -0.046672558858340096 2216 48-49 0.022009359922087413 2216 50-51 -0.05273035897447187 2216 52-53 0.056208261485494404 2216 54-55 0.014537939734040606 2216 56-57 0.13621628171510736 2216 58-59 -0.10120958088813126 2216 60-61 -0.04468392492822204 2216 62-63 0.015045121411475293 2216 64-65 0.1178824214111458 2216 66-67 -0.02274697683535365 2216 68-69 -0.1274169086864596 2216 70-71 -0.10820890825358731 2216 72-73 -0.1210796788973667 2216 74-75 -0.036520055228891124 2216 76-77 -0.13629708200155477 2216 78-79 0.033413724765647856 2216 80-81 0.12769958607495013 2216 82-83 0.03326010984342531 2216 84-85 0.0482905606250128 2216 86-87 -0.041209269884063815 2216 88-89 -0.08493967490490562 2216 90-91 0.016994302795396266 2216 92-93 0.2005316723215671 2216 94-95 0.12437322414556462 2216 96-97 0.1353478072410006 2216 98-99 0.13671541901050333 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 4.0 7 260.0 8 1705.0 9 3584.0 10 5848.0 11 11202.0 12 19537.0 13 30931.0 14 39700.0 15 43280.0 16 47401.0 17 51192.0 18 54793.0 19 59161.0 20 64536.0 21 71310.0 22 78234.0 23 88398.0 24 98734.0 25 110359.0 26 124103.0 27 142118.0 28 166174.0 29 199698.0 30 243703.0 31 302337.0 32 381171.0 33 487806.0 34 633953.0 35 852000.0 36 1121263.0 37 1037255.0 38 228741.0 39 112.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.038339205000135 18.264736442596334 12.274018764041342 25.422905588362188 2 15.809024582084854 20.72698553348872 37.55006725138932 25.9139226330371 3 18.314380092568356 26.12666595692495 28.4923409654095 27.066612985097187 4 13.03878494304108 17.351049605454104 35.73519877575562 33.8749666757492 5 14.44649834727891 37.427416362931346 33.206614178183905 14.919471111605839 6 32.26666811751841 36.047259338620414 17.55248762499443 14.133584918866754 7 28.12725577423061 31.652266718113086 21.86139964353161 18.359077864124696 8 27.294461976386508 33.2611975732152 20.02201863570039 19.422321814697902 9 27.486033223818534 15.358638050184666 18.828300960958902 38.3270277650379 10-11 25.46247001920271 25.724726175017125 26.56596775315365 22.24683605262651 12-13 26.437854113819025 23.51987757556205 26.136174983306624 23.9060933273123 14-15 24.649917073530098 24.555587203075962 23.281633996279446 27.51286172711449 16-17 24.335417339860697 26.804080961944184 24.759725333159817 24.100776365035305 18-19 25.039103150117718 26.150247264838132 25.648041798646386 23.162607786397764 20-21 25.851012097646393 25.670792148727333 24.6472616522799 23.830934101346372 22-23 24.83738574358774 25.620625112214313 24.234865643531904 25.307123500666044 24-25 24.485235339894228 26.229439853465113 24.08067182997436 25.204652976666296 26-27 25.04613195216129 25.70840430132572 24.12504424241328 25.12041950409971 28-29 24.59514839099431 25.58753198441498 24.995310655903026 24.82200896868768 30-31 25.152121585218634 25.790678963588825 24.5878227590993 24.469376692093235 32-33 24.188179907988978 25.990778605735954 24.410491223676857 25.410550262598207 34-35 24.444275178346384 26.105687284527303 25.00394634267959 24.446091194446726 36-37 25.054998799371177 25.853927953153566 24.383477465160077 24.707595782315188 38-39 25.247320053036486 25.428701080977987 24.35311967280539 24.970859193180132 40-41 24.612023374986013 26.080915177668807 24.75198155222412 24.555079895121064 42-43 25.428605375141 25.674127720732997 24.487402073022054 24.40986483110395 44-45 25.01639192877455 25.769508968542937 24.29691161210263 24.917187490579877 46-47 24.31527027823856 25.879881534034553 25.409033581286838 24.395814606440048 48-49 25.149084502159702 25.964712323836043 24.834391195057428 24.051811978946823 50-51 24.0049375033361 26.33100329485488 24.889182621011695 24.77487658079732 52-53 23.99487957170857 26.03301501352159 24.414232090889588 25.557873323880248 54-55 24.39599106138088 26.016104748358345 24.396233686924525 25.19167050333625 56-57 24.688200443401858 25.268604857539838 24.321291803094518 25.721902895963783 58-59 24.372390213044344 25.31897539085872 25.117772644572838 25.19086175152409 60-61 24.61106757738983 25.563181676683673 24.75068754932467 25.075063196601832 62-63 23.669459899364806 25.810637674335645 24.798043644070976 25.721858782228573 64-65 23.627838590195605 25.55110921781495 25.01845056975095 25.802601622238498 66-67 24.629356233273537 25.423153839367114 24.71917183777902 25.22831808958033 68-69 24.815931763698014 24.932208217418367 24.661438110708712 25.59042190817491 70-71 23.760187148110248 25.268913653686297 24.98273314881048 25.988166049392973 72-73 24.61117786172785 25.15885723663034 24.882205298559555 25.347759603082253 74-75 24.902872583504728 24.746577619661082 24.91200412669288 25.43854567014131 76-77 24.156821093658902 25.067630032219203 25.677598001236067 25.097950872885832 78-79 24.463742406371907 24.997327442875285 25.51414190771024 25.02478824304257 80-81 23.788625802741315 24.888910586311244 25.872904211582416 25.44955939936503 82-83 23.848370504792 25.261267272916825 24.973248695740658 25.917113526550512 84-85 24.00513601514454 25.294676075048052 24.76270118987978 25.937486719927634 86-87 24.124640123824314 24.53377296101537 25.11207462044175 26.22951229471857 88-89 24.132654119053853 24.24936435783709 25.581415353903175 26.036566169205877 90-91 24.595223394160783 24.582673036493972 25.200140340496276 25.62196322884897 92-93 23.88046324715617 24.770384332095258 25.251481375989748 26.097671044758826 94-95 23.30052202723788 24.945140894123654 25.794572922430554 25.959764156207914 96-97 24.33232023689664 24.5797247685242 25.22855693825974 25.859398056319417 98-99 24.22141095429332 24.56979182581309 25.023339842069888 26.185457377823703 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 720.0 1 683.5 2 681.0 3 685.5 4 607.0 5 546.0 6 508.5 7 487.0 8 505.5 9 509.5 10 490.5 11 477.5 12 491.0 13 501.5 14 545.5 15 683.5 16 777.0 17 939.5 18 1218.5 19 1583.5 20 2241.5 21 3207.0 22 4784.5 23 6946.5 24 9716.5 25 13128.5 26 17268.5 27 21715.5 28 27200.5 29 33842.5 30 40033.0 31 46661.0 32 54202.0 33 62664.0 34 70870.5 35 80052.0 36 89161.5 37 98484.5 38 110213.0 39 117226.5 40 124778.5 41 135548.5 42 151247.0 43 174050.0 44 194913.5 45 215519.5 46 249390.0 47 282855.0 48 308186.0 49 352805.0 50 404009.0 51 377873.0 52 320885.0 53 305194.5 54 291408.0 55 278077.5 56 253672.5 57 234254.0 58 222473.0 59 198366.5 60 168730.5 61 132061.5 62 106658.0 63 86711.0 64 65408.5 65 50540.5 66 36886.0 67 27941.0 68 22231.0 69 18513.5 70 16301.0 71 14542.5 72 14043.5 73 12408.0 74 8959.5 75 6445.5 76 4571.0 77 3159.5 78 2064.0 79 1276.0 80 838.0 81 594.0 82 389.0 83 257.5 84 178.5 85 136.0 86 90.0 87 60.0 88 49.0 89 33.5 90 26.0 91 19.0 92 11.0 93 13.5 94 13.0 95 7.5 96 3.5 97 3.5 98 4.5 99 9.5 100 15.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.002190982182021212 2 0.0 3 1.0293204881978847E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 7.352289201413463E-6 18-19 0.0 20-21 3.529098816678462E-4 22-23 0.0 24-25 0.004573123883279174 26-27 2.9409156805653852E-5 28-29 0.008991849693328665 30-31 5.808308469116636E-4 32-33 6.617060281272117E-5 34-35 3.6761446007067314E-5 36-37 0.0 38-39 2.9409156805653852E-5 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 1.985118084381635E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 4.4113735208480775E-5 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 6800603.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.429751913565134 #Duplication Level Percentage of deduplicated Percentage of total 1 78.20183197282242 34.744879937388056 2 11.824467915982941 10.507163520340653 3 3.996825499980397 5.3273389621781995 4 1.7671122392974408 3.1404943358163933 5 0.9807147206656976 2.1786455868579138 6 0.6284617195281207 1.6753438971484174 7 0.4230222019185048 1.315634003961846 8 0.30655547839794517 1.0896147082371983 9 0.24539378380422505 0.9812506441997481 >10 1.3713952328952492 11.308343554238814 >50 0.12515464817865962 3.9021407455480306 >100 0.1070977433384528 9.607972961698033 >500 0.013093738886355193 4.005530748884543 >1k 0.008474313099093697 7.354955335087736 >5k 1.3293040153418819E-4 0.3939049892972267 >10k+ 2.6586080306837637E-4 2.466786069117216 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 46357 0.6816601410198477 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 42561 0.6258415614027167 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 19825 0.29151826683604376 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 12915 0.18990963007250974 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 12810 0.1883656493402129 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 11337 0.16670580535284887 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 10715 0.1575595575862905 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 10548 0.1551038929930184 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 7665 0.11271059345766839 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 7323 0.10768162764390157 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.4704578402826926E-5 2 0.0 0.0 0.0 0.0 2.9409156805653852E-5 3 0.0 0.0 0.0 0.0 2.9409156805653852E-5 4 0.0 0.0 0.0 1.4704578402826926E-5 2.9409156805653852E-5 5 0.0 0.0 0.0 2.9409156805653852E-5 2.9409156805653852E-5 6 0.0 0.0 0.0 2.9409156805653852E-5 4.4113735208480775E-5 7 0.0 1.4704578402826926E-5 0.0 2.9409156805653852E-5 4.4113735208480775E-5 8 0.0 1.4704578402826926E-5 0.0 2.9409156805653852E-5 4.4113735208480775E-5 9 0.0 1.4704578402826926E-5 0.0 4.4113735208480775E-5 4.4113735208480775E-5 10-11 7.352289201413463E-6 1.4704578402826926E-5 0.0 8.087518121554809E-5 4.4113735208480775E-5 12-13 1.4704578402826926E-5 1.4704578402826926E-5 0.0 1.0293204881978849E-4 5.8818313611307704E-5 14-15 1.4704578402826926E-5 1.4704578402826926E-5 0.0 1.1763662722261541E-4 1.0293204881978849E-4 16-17 2.9409156805653852E-5 1.4704578402826926E-5 0.0 1.4704578402826925E-4 1.4704578402826925E-4 18-19 6.617060281272117E-5 2.9409156805653852E-5 0.0 1.9115951923675005E-4 1.4704578402826925E-4 20-21 7.352289201413463E-5 2.9409156805653852E-5 0.0 2.7203470045229815E-4 1.4704578402826925E-4 22-23 8.822747041696155E-5 4.4113735208480775E-5 0.0 9.925590421908175E-4 1.6910265163250964E-4 24-25 8.822747041696155E-5 5.146602440989424E-5 0.0 0.0028526882101484238 1.764549408339231E-4 26-27 1.1763662722261541E-4 5.8818313611307704E-5 0.0 0.00511719328418377 1.764549408339231E-4 28-29 1.1763662722261541E-4 5.8818313611307704E-5 0.0 0.014844271897653782 1.764549408339231E-4 30-31 1.3234120562544233E-4 5.8818313611307704E-5 0.0 0.04426813328171046 1.764549408339231E-4 32-33 1.396934948268558E-4 5.8818313611307704E-5 0.0 0.09613853359768244 2.1321638684099043E-4 34-35 1.4704578402826925E-4 6.617060281272117E-5 0.0 0.16691166945048844 2.205686760424039E-4 36-37 1.6175036243109618E-4 7.352289201413463E-5 0.0 0.2756814358961992 2.205686760424039E-4 38-39 1.764549408339231E-4 7.352289201413463E-5 0.0 0.4451443496995781 2.205686760424039E-4 40-41 1.764549408339231E-4 7.352289201413463E-5 0.0 0.6562506295397629 2.205686760424039E-4 42-43 1.8380723003533656E-4 7.352289201413463E-5 0.0 0.8414694991017708 2.205686760424039E-4 44-45 1.9115951923675002E-4 8.822747041696156E-5 0.0 1.0583840874110721 2.4997783284805774E-4 46-47 1.9115951923675002E-4 1.0293204881978849E-4 0.0 1.3011713814201475 2.6468241125088466E-4 48-49 1.9115951923675002E-4 1.5439807322968272E-4 0.0 1.5517888634287282 2.793869896537116E-4 50-51 1.9115951923675002E-4 1.8380723003533656E-4 0.0 1.8254116583485318 2.793869896537116E-4 52-53 1.9115951923675002E-4 1.9115951923675002E-4 0.0 2.104629545350611 3.01443857257952E-4 54-55 1.9115951923675002E-4 1.9115951923675002E-4 0.0 2.379833082448718 3.382053032650193E-4 56-57 1.9115951923675002E-4 1.9115951923675002E-4 0.0 2.6899820501211433 3.382053032650193E-4 58-59 1.9115951923675002E-4 1.9115951923675002E-4 0.0 3.0274888859120286 3.529098816678462E-4 60-61 1.9115951923675002E-4 1.9115951923675002E-4 0.0 3.3693850383561577 3.8231903847350004E-4 62-63 2.0586409763957697E-4 1.9115951923675002E-4 0.0 3.713869196599184 4.2643277368198087E-4 64-65 2.0586409763957697E-4 1.9115951923675002E-4 0.0 4.07562388217633 4.2643277368198087E-4 66-67 2.0586409763957697E-4 1.9115951923675002E-4 0.0 4.464971709126382 4.2643277368198087E-4 68-69 2.205686760424039E-4 1.9115951923675002E-4 0.0 4.892294109801734 4.411373520848078E-4 70-71 2.205686760424039E-4 1.9851180843816348E-4 0.0 5.389595599096139 4.558419304876347E-4 72-73 2.205686760424039E-4 2.0586409763957697E-4 0.0 5.856171871817837 4.999556656961155E-4 74-75 2.205686760424039E-4 2.0586409763957697E-4 0.0 6.310638924224808 4.999556656961155E-4 76-77 2.205686760424039E-4 2.0586409763957697E-4 0.0 6.799588212986407 4.999556656961155E-4 78-79 2.205686760424039E-4 2.4262554364664428E-4 0.0 7.3059330179985515 4.999556656961155E-4 80-81 2.205686760424039E-4 2.6468241125088466E-4 0.0 7.854979918692504 5.367171117031829E-4 82-83 2.205686760424039E-4 2.6468241125088466E-4 0.0 8.438053213810598 5.734785577102501E-4 84-85 2.205686760424039E-4 2.6468241125088466E-4 0.0 8.999819574822997 5.734785577102501E-4 86-87 2.205686760424039E-4 2.6468241125088466E-4 0.0 9.576680185565898 5.734785577102501E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACTAG 1605 0.0 20.28048 1 GTATAAG 2140 0.0 19.121595 1 GTACATA 1865 0.0 18.450499 1 CATGGGG 14660 0.0 18.428408 4 GTACAAG 3360 0.0 17.991165 1 GTACAAA 3855 0.0 16.887247 1 GTATTAT 1890 0.0 16.23818 1 GTCCTAC 1990 0.0 16.1232 1 GTATAAT 2235 0.0 16.020218 1 CTAGTAC 2350 0.0 15.82952 3 ATATACG 3095 0.0 15.474699 6 GTATATG 2470 0.0 15.249068 1 GTATAGA 1500 0.0 15.19008 1 TACGCTA 3100 0.0 15.149746 9 GTATAAA 7320 0.0 14.991881 1 TAGTACT 2425 0.0 14.95645 4 GTATTAG 2730 0.0 14.818759 1 GTACATG 79995 0.0 14.450852 1 TCTAACG 840 0.0 14.392615 2 TACATGG 78335 0.0 14.376856 2 >>END_MODULE