FastQCFastQC Report
Fri 27 May 2016
ERR765550_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765550_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6800603
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA469600.6905270017967524No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT465750.6848657391116642No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG198870.29242995069701905No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC143680.21127538249181727No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140190.20614348462923066No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC137160.2016879973731741No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG115010.16911735621091248No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT112540.1654853253454142No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG112310.1651471200421492No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90970.13376754973051652No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC86610.127356353546884No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG79000.11616616938233272No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72420.10649055679327259No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG5300.033.3415372
AGTACTT234350.021.68995512-13
TCACGCA9800.019.9297164
TCTAACG6800.019.1481592
TAACGCA4450.018.810074
GAGTACT227300.018.6901412-13
TACTTTT274700.018.47024714-15
ATCACGC10350.018.4213473
TATTCGC2906.663504E-817.6377759
GTATAAG23700.017.4722771
CTAACGC7300.017.1996173
CATGGGG127150.017.1162014
TAGTACT19950.016.549824
GTACTTT259150.016.41115414-15
TACGGGC12650.016.174844
GTATATA16200.015.7963061
GTATAAC11250.015.7158891
ACTTTTT279500.015.38980116-17
ATACGGG13900.015.3893733
ATGGGTA31100.015.2516785
Produced by FastQC (version 0.11.5)