##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765550_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6800603 Sequences flagged as poor quality 0 Sequence length 99 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.33516969009954 33.0 33.0 33.0 33.0 33.0 2 32.2948800275505 33.0 33.0 33.0 33.0 33.0 3 32.337839747445926 33.0 33.0 33.0 33.0 33.0 4 36.195956887940675 37.0 37.0 37.0 33.0 37.0 5 36.1744536477133 37.0 37.0 37.0 33.0 37.0 6 36.19908616927058 37.0 37.0 37.0 33.0 37.0 7 36.09128528749583 37.0 37.0 37.0 33.0 37.0 8 36.10110838700627 37.0 37.0 37.0 33.0 37.0 9 36.119263982914454 37.0 37.0 37.0 33.0 37.0 10-11 36.07545007405961 37.0 37.0 37.0 33.0 37.0 12-13 36.06031215173125 37.0 37.0 37.0 33.0 37.0 14-15 37.559944610794076 40.0 37.0 40.0 33.0 40.0 16-17 37.54165880584413 40.0 37.0 40.0 33.0 40.0 18-19 37.57262400113637 40.0 37.0 40.0 33.0 40.0 20-21 37.55755636669278 40.0 37.0 40.0 33.0 40.0 22-23 37.5397975003099 40.0 37.0 40.0 33.0 40.0 24-25 37.53077859419231 40.0 37.0 40.0 33.0 40.0 26-27 37.41434715421559 40.0 37.0 40.0 33.0 40.0 28-29 37.31281012874888 37.0 37.0 40.0 33.0 40.0 30-31 37.167777622072634 37.0 37.0 40.0 33.0 40.0 32-33 37.05276899710216 37.0 37.0 40.0 33.0 40.0 34-35 36.97593279595942 37.0 37.0 40.0 33.0 40.0 36-37 36.85484059281214 37.0 37.0 40.0 33.0 40.0 38-39 36.7468140692818 37.0 37.0 40.0 33.0 40.0 40-41 36.59094296491061 37.0 37.0 40.0 33.0 40.0 42-43 36.45130365645517 37.0 37.0 40.0 33.0 40.0 44-45 36.200629267728175 37.0 37.0 40.0 33.0 40.0 46-47 35.99617519211164 37.0 37.0 40.0 33.0 40.0 48-49 35.76675112486349 37.0 37.0 40.0 30.0 40.0 50-51 35.58968013571737 37.0 37.0 40.0 27.0 40.0 52-53 35.45278212829068 37.0 35.0 40.0 27.0 40.0 54-55 35.261758111743916 37.0 33.0 40.0 27.0 40.0 56-57 35.06537265886569 37.0 33.0 40.0 27.0 40.0 58-59 34.85914991655887 37.0 33.0 40.0 27.0 40.0 60-61 34.68404110635483 37.0 33.0 37.0 27.0 40.0 62-63 34.4484925822019 37.0 33.0 37.0 27.0 40.0 64-65 34.23926503282135 37.0 33.0 37.0 27.0 40.0 66-67 34.011574561844 37.0 33.0 37.0 27.0 40.0 68-69 33.80159377043476 37.0 33.0 37.0 27.0 40.0 70-71 33.5831082184918 37.0 33.0 37.0 27.0 38.5 72-73 33.352926571364335 37.0 33.0 37.0 27.0 37.0 74-75 33.11556850179315 37.0 33.0 37.0 27.0 37.0 76-77 32.925222072219185 37.0 33.0 37.0 27.0 37.0 78-79 32.74961793829165 33.0 33.0 37.0 27.0 37.0 80-81 32.58967785650772 33.0 33.0 37.0 27.0 37.0 82-83 32.49899891230234 33.0 33.0 37.0 27.0 37.0 84-85 32.35862694822798 33.0 33.0 37.0 22.0 37.0 86-87 32.24084283408398 33.0 33.0 37.0 22.0 37.0 88-89 32.11621035664044 33.0 33.0 37.0 22.0 37.0 90-91 32.009973306778825 33.0 33.0 37.0 22.0 37.0 92-93 31.914016154155743 33.0 33.0 37.0 22.0 37.0 94-95 31.822134596005675 33.0 33.0 37.0 22.0 37.0 96-97 31.774975983747325 33.0 33.0 37.0 22.0 37.0 98-99 30.9614767249316 33.0 30.0 37.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -1.7864066345651963E-4 1101 2 0.09284086241846978 1101 3 0.08591607017453384 1101 4 0.12310341381230216 1101 5 0.07765320858859326 1101 6 0.09245979120861847 1101 7 0.12574985024647134 1101 8 0.13443703729822687 1101 9 0.0839786839520471 1101 10-11 0.0803718588486646 1101 12-13 0.08666321948155087 1101 14-15 0.15071667243790188 1101 16-17 0.19240725907464906 1101 18-19 0.18622113880451963 1101 20-21 0.2258878027210045 1101 22-23 0.19183350917193565 1101 24-25 0.2199212200698497 1101 26-27 0.17482813614853399 1101 28-29 0.2259514955394195 1101 30-31 0.18540583728867688 1101 32-33 0.27135179500677964 1101 34-35 0.27532497938209843 1101 36-37 0.30233804334189784 1101 38-39 0.18681054402047437 1101 40-41 0.09824095561658908 1101 42-43 0.19367604206490086 1101 44-45 0.2049584686797914 1101 46-47 0.265098298222469 1101 48-49 0.23410327082359572 1101 50-51 0.2374303750260438 1101 52-53 0.21066926974529565 1101 54-55 0.18751905216219455 1101 56-57 0.2864007491201974 1101 58-59 0.28361020380710045 1101 60-61 0.1762361125378149 1101 62-63 0.23382622065105352 1101 64-65 0.23020356314889057 1101 66-67 0.37175806758462215 1101 68-69 0.32856469754118933 1101 70-71 0.19835660858007031 1101 72-73 0.1528994751583852 1101 74-75 0.23532901166460363 1101 76-77 0.2750768459042803 1101 78-79 0.25525997586583316 1101 80-81 0.220252915082348 1101 82-83 0.21645047993970223 1101 84-85 0.2206218245469529 1101 86-87 0.2279616848139483 1101 88-89 0.23352331865130083 1101 90-91 0.30462165432552624 1101 92-93 0.2658265185191233 1101 94-95 0.3148915346759651 1101 96-97 0.2868504099567417 1101 98-99 0.31961838670449083 1102 1 0.0013042389639608132 1102 2 0.1271097016856899 1102 3 0.08365044503883468 1102 4 0.07929564484128804 1102 5 0.07350201224426911 1102 6 0.10268392059918341 1102 7 0.029474626065727705 1102 8 0.141057928073117 1102 9 0.07530894387612364 1102 10-11 0.06676787011289775 1102 12-13 0.04332691411435974 1102 14-15 0.17575956786598823 1102 16-17 0.13813340308426092 1102 18-19 0.19204606775929278 1102 20-21 0.20608562806374664 1102 22-23 0.17330777063121872 1102 24-25 0.22879104566664665 1102 26-27 0.1442118937015593 1102 28-29 0.09352980955038248 1102 30-31 0.0631061846813239 1102 32-33 0.09147094213476237 1102 34-35 0.167778190617355 1102 36-37 0.20417563367444558 1102 38-39 0.1933543600968477 1102 40-41 0.1938686607596125 1102 42-43 0.30067746916225246 1102 44-45 0.3681934664855149 1102 46-47 0.37564743654537835 1102 48-49 0.35384310791404516 1102 50-51 0.35757300754015375 1102 52-53 0.33451670823148305 1102 54-55 0.2328348353536569 1102 56-57 0.2744414548697378 1102 58-59 0.2797911097784791 1102 60-61 0.19662805095010327 1102 62-63 0.3064067217728166 1102 64-65 0.26959660251772277 1102 66-67 0.3749071890057465 1102 68-69 0.25306059247921553 1102 70-71 0.256713393944068 1102 72-73 0.2339055166743691 1102 74-75 0.34867160157687493 1102 76-77 0.43704862390528376 1102 78-79 0.33168210482788396 1102 80-81 0.3388865327123938 1102 82-83 0.3360522490525497 1102 84-85 0.252028119234609 1102 86-87 0.32622114941133873 1102 88-89 0.4006187161802117 1102 90-91 0.29503348914004945 1102 92-93 0.3717961009508848 1102 94-95 0.4000609929045993 1102 96-97 0.4048594388500888 1102 98-99 0.3699529840710625 1103 1 0.016947037037724044 1103 2 0.1254415852746078 1103 3 0.1252857693607865 1103 4 0.09169898553281541 1103 5 0.00935777123078907 1103 6 0.08001529241150962 1103 7 0.054239616140051794 1103 8 0.2035762451709502 1103 9 0.10338594274860924 1103 10-11 0.13508080578161952 1103 12-13 0.15339015932852362 1103 14-15 0.18770803057740437 1103 16-17 0.16883106082254784 1103 18-19 0.19527325998914336 1103 20-21 0.14335186565210023 1103 22-23 0.18102768864189756 1103 24-25 0.16072183987614608 1103 26-27 0.21606199165432116 1103 28-29 0.15993717768544968 1103 30-31 0.17872623073500193 1103 32-33 0.16969374967705875 1103 34-35 0.28589771972942657 1103 36-37 0.24590279581918395 1103 38-39 0.17677278828085718 1103 40-41 0.17944882785135974 1103 42-43 0.1858098296470274 1103 44-45 0.17840057270225884 1103 46-47 0.1686345426171556 1103 48-49 0.1892177174559606 1103 50-51 0.20521503872401325 1103 52-53 0.14381526504858755 1103 54-55 0.1266090560712101 1103 56-57 0.12202928363974053 1103 58-59 0.23202110706678525 1103 60-61 0.11312795624901639 1103 62-63 0.16344968365481805 1103 64-65 0.21661030428465722 1103 66-67 0.21622302815113414 1103 68-69 0.21719987174433442 1103 70-71 0.07801256009283719 1103 72-73 0.1450851070078869 1103 74-75 0.2656456759033645 1103 76-77 0.3023694684579681 1103 78-79 0.2536772114079682 1103 80-81 0.25114635209454406 1103 82-83 0.17954346588087589 1103 84-85 0.14487130211952604 1103 86-87 0.15761716719897123 1103 88-89 0.24623424997574972 1103 90-91 0.14327102293987437 1103 92-93 0.20462269585528858 1103 94-95 0.1578717718880469 1103 96-97 0.22568525736060252 1103 98-99 0.1667469047951471 1104 1 0.032305450275799785 1104 2 0.06659702061860884 1104 3 0.005030758394347856 1104 4 0.0058970889199585486 1104 5 -0.02152548690462197 1104 6 0.07846726852749697 1104 7 0.055204749235706174 1104 8 0.08696867141275533 1104 9 0.04671697296484467 1104 10-11 -0.03917923505761678 1104 12-13 -0.08548573596222298 1104 14-15 0.011454549395764957 1104 16-17 0.0510049993485282 1104 18-19 0.03434499931879742 1104 20-21 0.07636683920024012 1104 22-23 0.12859853711083247 1104 24-25 0.03474641949116375 1104 26-27 0.022393867355994246 1104 28-29 -0.004275448388526115 1104 30-31 -0.0499015086311303 1104 32-33 0.011860693402361733 1104 34-35 -0.010152014921530395 1104 36-37 0.0666598932332434 1104 38-39 0.03833883493970802 1104 40-41 0.001137504188989169 1104 42-43 0.0729414214269255 1104 44-45 0.016053945064761876 1104 46-47 -0.03021763871079486 1104 48-49 -0.019691948132617654 1104 50-51 -0.0744936982726685 1104 52-53 0.0173802901332607 1104 54-55 -0.08591114223361984 1104 56-57 0.040724271664963396 1104 58-59 0.003070930688892304 1104 60-61 -0.01919017578239135 1104 62-63 -0.06798919653796531 1104 64-65 -0.06910283619085078 1104 66-67 0.09118044711435402 1104 68-69 0.08007623176104772 1104 70-71 -0.07495379276021907 1104 72-73 0.05797929647253852 1104 74-75 0.10830470886998711 1104 76-77 0.0895504336659485 1104 78-79 0.08461391988910094 1104 80-81 0.11539093085988839 1104 82-83 0.010265892905621854 1104 84-85 0.06061039047514072 1104 86-87 0.07756955391682396 1104 88-89 0.08829759791716185 1104 90-91 0.01807787213185108 1104 92-93 0.13367415291358142 1104 94-95 -0.006008760097152788 1104 96-97 0.06581955319620292 1104 98-99 0.09970235571650932 1105 1 -0.06431641007476685 1105 2 0.11191620258729529 1105 3 0.06920549188248515 1105 4 0.03891407389725288 1105 5 0.08437685026673591 1105 6 0.00777679833758782 1105 7 0.032090153585315306 1105 8 0.07160355027140497 1105 9 0.014094400459448764 1105 10-11 0.011440883452415562 1105 12-13 -0.02952975269936786 1105 14-15 0.058337597550853104 1105 16-17 0.0575923303781849 1105 18-19 0.0861352427976314 1105 20-21 0.07520310494013671 1105 22-23 0.07418551460096978 1105 24-25 0.06465929472967247 1105 26-27 0.036444796725035644 1105 28-29 0.07148633880009925 1105 30-31 0.05030452781712569 1105 32-33 0.09170345383100198 1105 34-35 0.08005270954642185 1105 36-37 0.10681289985662801 1105 38-39 0.07411220613840186 1105 40-41 -0.035287673320063107 1105 42-43 0.07052147153596167 1105 44-45 0.11380188047046147 1105 46-47 0.10527087515352207 1105 48-49 0.13175803654439022 1105 50-51 0.13312671702372114 1105 52-53 0.09993868337190293 1105 54-55 0.09013292853489929 1105 56-57 0.08050402871725026 1105 58-59 0.16582132509216763 1105 60-61 0.056885395203060796 1105 62-63 0.06876306387869136 1105 64-65 0.11201222974213465 1105 66-67 0.2103270131171513 1105 68-69 0.15878618160346036 1105 70-71 0.13455269538149395 1105 72-73 0.10938176646030229 1105 74-75 0.1534243903842949 1105 76-77 0.1842474171558166 1105 78-79 0.14483766439346368 1105 80-81 0.09229723144763824 1105 82-83 0.09238495020859716 1105 84-85 -0.0015916415826353614 1105 86-87 0.12824343253963377 1105 88-89 0.19490039813996418 1105 90-91 0.2073741670141196 1105 92-93 0.17272888636346906 1105 94-95 0.12438166015878949 1105 96-97 0.050475925985164594 1105 98-99 0.2289562459018093 1106 1 -0.02535027155482794 1106 2 0.040227407491443046 1106 3 0.039803858214305876 1106 4 0.04303848310810565 1106 5 0.052820387105725786 1106 6 0.06261974977935125 1106 7 -0.004577948041152524 1106 8 0.048927451972545555 1106 9 -0.05855257659645474 1106 10-11 0.010528806519815248 1106 12-13 0.030272309602636938 1106 14-15 0.03444937215351018 1106 16-17 -0.004246992469404631 1106 18-19 0.004107716567681052 1106 20-21 0.018288254437301532 1106 22-23 0.028227874448269574 1106 24-25 -0.007873783744990703 1106 26-27 -0.012899215984568002 1106 28-29 -0.036920044736518776 1106 30-31 -0.018938540989772434 1106 32-33 -0.07255656196653604 1106 34-35 0.007024704311163532 1106 36-37 0.030387727400828624 1106 38-39 -0.03289665172630407 1106 40-41 0.0011904575571435316 1106 42-43 -0.12604545748882146 1106 44-45 -0.0816842384939136 1106 46-47 -0.016952010500126846 1106 48-49 -0.07287630283716595 1106 50-51 -0.031223538374241855 1106 52-53 -0.028489587269589833 1106 54-55 -0.04790091988695622 1106 56-57 0.0686121420802337 1106 58-59 0.060849840499280106 1106 60-61 -0.031141538141035596 1106 62-63 0.01684142617472162 1106 64-65 -0.0559419868879516 1106 66-67 0.0416535129684803 1106 68-69 -0.01098702603056978 1106 70-71 -0.13339504715584383 1106 72-73 -0.07169902718717935 1106 74-75 -0.007510801377087262 1106 76-77 0.03327551957009689 1106 78-79 -0.014787301396388841 1106 80-81 -0.011761711780586381 1106 82-83 -0.15483334072398947 1106 84-85 -0.11494890696481974 1106 86-87 -0.04104115198450131 1106 88-89 -0.042957066199498684 1106 90-91 -0.06762650844144247 1106 92-93 -0.02338819286496374 1106 94-95 -0.059248924122712765 1106 96-97 -0.00681571419083582 1106 98-99 0.02004508243041414 1107 1 -0.008719545620408553 1107 2 0.08238129937159755 1107 3 0.014349586433048955 1107 4 0.005617811307956799 1107 5 0.04995075771176971 1107 6 0.023280757457655454 1107 7 0.059436808466429625 1107 8 0.04946166468023705 1107 9 0.053918473177319015 1107 10-11 0.00980641689955064 1107 12-13 -0.022642909125380584 1107 14-15 0.08972476419773301 1107 16-17 0.006584749307343429 1107 18-19 0.015170632218236335 1107 20-21 0.036925858895251906 1107 22-23 0.018370535943020627 1107 24-25 -0.011109669795736465 1107 26-27 0.027754459487276506 1107 28-29 -0.004250674197884052 1107 30-31 0.027303271727454614 1107 32-33 0.05408659745476996 1107 34-35 0.008735997514754956 1107 36-37 0.08401049945444328 1107 38-39 -0.015212822962112682 1107 40-41 -0.04032445686237196 1107 42-43 0.012948077281897952 1107 44-45 0.04013657697957029 1107 46-47 0.09715763640171815 1107 48-49 -0.007775508340323256 1107 50-51 0.07782045995730869 1107 52-53 0.04047221586752414 1107 54-55 -0.07624040889738382 1107 56-57 0.062482276721645746 1107 58-59 0.06607200402078206 1107 60-61 0.04684852164763953 1107 62-63 0.044538666312689656 1107 64-65 0.0184594490814618 1107 66-67 0.05550765907094046 1107 68-69 -0.05119507332167217 1107 70-71 0.020755477893374064 1107 72-73 -0.08167204988751564 1107 74-75 0.12727703288957315 1107 76-77 0.05906486349606155 1107 78-79 0.04984950080528705 1107 80-81 -0.03164406190907698 1107 82-83 -0.03570527736511764 1107 84-85 0.0559420512561104 1107 86-87 -0.043450113545020486 1107 88-89 -0.0033761662365847656 1107 90-91 0.0445221569604648 1107 92-93 0.17087211333776153 1107 94-95 0.08291275194923386 1107 96-97 0.05932037604551965 1107 98-99 0.008335744646927878 1108 1 -0.05760175432320125 1108 2 0.017232815367805188 1108 3 0.012869511126503141 1108 4 -0.02722000395144164 1108 5 0.01930757004627992 1108 6 0.005268533437757128 1108 7 -0.0632580865401593 1108 8 -0.11200666951648941 1108 9 -0.050694878313770175 1108 10-11 -0.09593976522822345 1108 12-13 -0.013850073070472035 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2215 86-87 -0.20255469688056138 2215 88-89 -0.11690715132352025 2215 90-91 -0.1628256338966061 2215 92-93 -0.1388588668300379 2215 94-95 -0.13195718839487114 2215 96-97 -0.1437217062474545 2215 98-99 -0.21629482923843923 2216 1 -0.14487287541635396 2216 2 -0.15205499225223207 2216 3 -0.10376422438847754 2216 4 -0.25251318003306267 2216 5 -0.18772245003671628 2216 6 -0.2858709069709633 2216 7 -0.1421247138248063 2216 8 -0.25915449936698565 2216 9 -0.17502954003237647 2216 10-11 -0.20064337851793823 2216 12-13 -0.15581473634433962 2216 14-15 -0.4634112546123177 2216 16-17 -0.40954129363572633 2216 18-19 -0.2872404873906689 2216 20-21 -0.3120131527155152 2216 22-23 -0.40111274292510757 2216 24-25 -0.4063602317841415 2216 26-27 -0.4479796948453796 2216 28-29 -0.3185066224688313 2216 30-31 -0.42592301298379454 2216 32-33 -0.317226947361533 2216 34-35 -0.33056173329707406 2216 36-37 -0.3609975896567761 2216 38-39 -0.43458980535777414 2216 40-41 -0.3804470737333361 2216 42-43 -0.3705276358065319 2216 44-45 -0.41595642742677086 2216 46-47 -0.5010257227084551 2216 48-49 -0.3484637254121168 2216 50-51 -0.32432314171122556 2216 52-53 -0.3357599273274019 2216 54-55 -0.29063533823861576 2216 56-57 -0.4117281609049215 2216 58-59 -0.47620840474898074 2216 60-61 -0.4504750538043396 2216 62-63 -0.40740778661632504 2216 64-65 -0.49215702701130226 2216 66-67 -0.4719889476457624 2216 68-69 -0.30121760513603846 2216 70-71 -0.4004726544354824 2216 72-73 -0.3965900087387979 2216 74-75 -0.4197275860308096 2216 76-77 -0.43412843566332526 2216 78-79 -0.3594162161971397 2216 80-81 -0.49080923482934224 2216 82-83 -0.4488076771009304 2216 84-85 -0.39253467887683513 2216 86-87 -0.47610300168561537 2216 88-89 -0.47625175612813564 2216 90-91 -0.536412937090585 2216 92-93 -0.3749084712799373 2216 94-95 -0.46305424225869274 2216 96-97 -0.47916672251927395 2216 98-99 -0.6175453384859608 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 3.0 9 16.0 10 47.0 11 89.0 12 238.0 13 439.0 14 1039.0 15 2683.0 16 5782.0 17 10653.0 18 17165.0 19 24281.0 20 31283.0 21 40105.0 22 49031.0 23 50127.0 24 46039.0 25 46449.0 26 55815.0 27 73095.0 28 97146.0 29 128679.0 30 170414.0 31 225752.0 32 297794.0 33 396701.0 34 530486.0 35 760340.0 36 1249816.0 37 1823985.0 38 664264.0 39 847.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.051206007400964 18.575693466945566 12.740920828586532 25.632179697066938 2 16.1405981204902 21.062911627101304 36.7811501421271 26.0153401102814 3 18.396353970375863 25.902497175618105 29.27136020144096 26.429788652565072 4 13.426162356485152 17.341168128767404 35.787488256556074 33.44518125819137 5 14.23372251498915 36.994231978461976 33.519272521077156 15.252772985471715 6 32.324442406063106 36.0757862207219 17.27680618909823 14.322965184116763 7 27.5966263579862 31.440711948631616 22.411233239170116 18.551428454212076 8 27.464035174527908 32.47534078963292 20.3055376118853 19.755086423953877 9 27.60169943753517 14.003787605305002 19.523548132422963 38.870964824736866 10-11 25.62605845393416 24.87433099682484 27.111228224908878 22.38838232433212 12-13 27.009773986218576 22.679694727070526 26.978063562892878 23.33246772381802 14-15 24.70900538746161 23.462018487577225 24.843708470501305 26.985267654459864 16-17 23.80891669260306 25.87456374106371 26.200196888647625 24.11632267768561 18-19 24.47742148179921 24.687668965939114 28.104653780565325 22.73025577169635 20-21 25.055502537279857 24.305920515719958 27.332834357019024 23.30574258998116 22-23 24.08790073468485 24.20514033829059 26.926531367880173 24.78042755914439 24-25 23.941472542949498 24.81546121718912 26.779125321681036 24.463940918180345 26-27 24.513826200411934 24.305087357694603 26.857522781435705 24.323563660457754 28-29 24.012716225311195 24.12731635709363 27.714829111477318 24.145138306117854 30-31 24.599257049482855 24.28730605995438 27.201686618986965 23.9117502715758 32-33 23.75826084833948 24.384396501310253 26.98363659810755 24.87370605224272 34-35 24.039537376317956 24.499614225385603 27.524059263568248 23.9367891347282 36-37 24.760230820708102 24.282604057316682 26.73186480669435 24.225300315280865 38-39 24.84270144868036 23.84424587055001 26.79257265863042 24.520480022139214 40-41 24.28089832622195 24.442619279496245 27.023603642206435 24.252878752075368 42-43 25.21564631842206 24.13147040049243 26.533992647416703 24.11889063366881 44-45 24.862464402053757 24.292956080512273 26.11595618800274 24.72862332943123 46-47 24.314174787147554 24.45050093352016 26.88939495512383 24.345929324208456 48-49 25.29554364517382 24.789595863778548 25.85434703363805 24.060513457409584 50-51 24.13164685543326 25.199794782903812 25.825621639728126 24.842936721934805 52-53 24.09242974483292 25.014120071411316 25.306608840421944 25.586841343333823 54-55 24.456559219822125 25.139086930967743 25.26206867243978 25.14228517677035 56-57 24.707037008335877 24.35795031705277 25.23895307519054 25.696059599420817 58-59 24.271362192841487 24.425797072935172 26.064483124359157 25.238357609864188 60-61 24.563057128904596 24.665099550731014 25.712793409643233 25.059049910721154 62-63 23.670202480574147 24.844715975921545 25.769516320832135 25.715565222672165 64-65 23.650160140211096 24.628720423762424 25.923987916953834 25.797131519072646 66-67 24.67146663317944 24.600186189371737 25.622191149814217 25.106156027634608 68-69 24.805109193993534 24.126926685765955 25.46212446161024 25.60583965863027 70-71 23.725616684285203 24.459742761046336 25.76445794586156 26.050182608806892 72-73 24.612280705108063 24.419172829232938 25.51428895349427 25.45425751216473 74-75 24.86481157068409 24.086733223044053 25.48148505415073 25.566970152121126 76-77 24.11681979544728 24.476538372971536 26.12962191243883 25.277019919142358 78-79 24.401731229215535 24.55415157026919 25.83468535834661 25.209431842168666 80-81 23.67871991332532 24.514873830287378 26.051803943239143 25.754602313148162 82-83 23.612790190280936 24.85090107825005 25.17988663504447 26.35642209642454 84-85 23.7314863135878 24.88045499668631 24.94517009365529 26.442888596070595 86-87 23.844691465187356 24.17315002374439 25.30022004826519 26.68193846280306 88-89 23.669682558950257 24.003947895368714 25.780294946663044 26.54607459901798 90-91 24.122209929088026 24.264727939411905 25.371389406494536 26.24167272500553 92-93 23.33131314453256 24.509495193712773 25.391810140387065 26.767381521367597 94-95 22.72062085593997 24.63810631703597 26.016171934404685 26.625100892619376 96-97 23.811769449492495 24.230695791377652 25.472424407720073 26.48511035140978 98-99 23.560576116583203 24.17418662676412 25.405370551401003 26.85986670525168 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 96.0 1 101.5 2 309.0 3 508.5 4 392.0 5 358.5 6 676.5 7 1748.5 8 3269.5 9 3308.0 10 2054.0 11 1403.0 12 1365.5 13 1503.0 14 1810.5 15 2216.5 16 2611.0 17 3023.5 18 3604.0 19 4339.0 20 5333.0 21 6660.5 22 8620.0 23 11085.5 24 13831.0 25 17307.5 26 21672.0 27 25898.5 28 30754.5 29 37056.5 30 42784.0 31 48887.5 32 55631.5 33 62895.0 34 70315.5 35 78579.5 36 86422.5 37 94905.0 38 105966.0 39 112665.5 40 119580.5 41 128869.0 42 143949.5 43 167231.5 44 188137.0 45 208794.0 46 244766.0 47 279683.5 48 302096.5 49 354107.5 50 420889.0 51 386534.0 52 315860.0 53 302299.0 54 290186.5 55 277800.0 56 254094.5 57 235692.5 58 225616.5 59 200857.0 60 169104.0 61 129838.0 62 105687.5 63 85206.0 64 62384.0 65 48739.5 66 35004.0 67 26253.0 68 20788.5 69 17375.0 70 15572.0 71 14218.5 72 14970.0 73 12618.5 74 7605.5 75 5658.0 76 4330.5 77 2719.5 78 1346.5 79 814.0 80 537.5 81 340.0 82 208.5 83 138.0 84 86.0 85 47.0 86 21.5 87 11.5 88 8.0 89 6.0 90 1.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.057641947339081546 2 0.0 3 0.0 4 0.0 5 0.005161307019392251 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0013307643454558368 16-17 0.004595180750883414 18-19 6.764106065300386E-4 20-21 1.9115951923675005E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 2.2792096524381736E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.9409156805653852E-5 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 3.6761446007067314E-5 76-77 1.3234120562544233E-4 78-79 2.2056867604240387E-5 80-81 1.4704578402826926E-5 82-83 7.352289201413463E-6 84-85 3.823190384735001E-4 86-87 7.278766309399328E-4 88-89 2.573301220494712E-4 90-91 8.675701257667887E-4 92-93 0.0012278322966360483 94-95 8.234563905583078E-4 96-97 0.0018160154327491252 98-99 5.146602440989423E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 6800603.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.24666172787154 #Duplication Level Percentage of deduplicated Percentage of total 1 74.10277890912953 29.082866969418603 2 13.9981970092233 10.987650056421801 3 4.691987538514163 5.52434543266362 4 2.149768486104431 3.374849462695164 5 1.1844742192251947 2.3243329503657986 6 0.7692856643693866 1.8115136544963746 7 0.512775141779996 1.4087298772330608 8 0.3766581235274076 1.1826059168908014 9 0.27701813333264064 0.9784833274253305 >10 1.6216241309912727 11.853098044946197 >50 0.153164779380346 4.197142042979611 >100 0.13359456522964744 10.552899453724288 >500 0.01673236252038826 4.496775152577452 >1k 0.011154856201788483 8.324196698229763 >5k 4.491888403220805E-4 1.1164107861957908 >10k+ 3.3689163024156035E-4 2.7841001737364035 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 46960 0.6905270017967524 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 46575 0.6848657391116642 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 19887 0.29242995069701905 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 14368 0.21127538249181727 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14019 0.20614348462923066 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 13716 0.2016879973731741 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 11501 0.16911735621091248 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 11254 0.1654853253454142 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 11231 0.1651471200421492 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9097 0.13376754973051652 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 8661 0.127356353546884 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 7900 0.11616616938233272 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7242 0.10649055679327259 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 1.4704578402826926E-5 3 0.0 0.0 0.0 0.0 1.4704578402826926E-5 4 0.0 0.0 0.0 1.4704578402826926E-5 1.4704578402826926E-5 5 0.0 0.0 0.0 2.9409156805653852E-5 1.4704578402826926E-5 6 0.0 0.0 0.0 2.9409156805653852E-5 2.9409156805653852E-5 7 1.4704578402826926E-5 0.0 0.0 4.4113735208480775E-5 2.9409156805653852E-5 8 1.4704578402826926E-5 0.0 0.0 4.4113735208480775E-5 2.9409156805653852E-5 9 1.4704578402826926E-5 1.4704578402826926E-5 0.0 7.352289201413463E-5 4.4113735208480775E-5 10-11 1.4704578402826926E-5 1.4704578402826926E-5 0.0 1.2498891642402887E-4 4.4113735208480775E-5 12-13 2.205686760424039E-5 2.9409156805653852E-5 0.0 1.5439807322968272E-4 5.8818313611307704E-5 14-15 2.9409156805653852E-5 2.9409156805653852E-5 0.0 1.764549408339231E-4 5.8818313611307704E-5 16-17 2.9409156805653852E-5 2.9409156805653852E-5 0.0 2.0586409763957697E-4 7.352289201413463E-5 18-19 2.9409156805653852E-5 2.9409156805653852E-5 0.0 2.4997783284805774E-4 8.822747041696155E-5 20-21 2.9409156805653852E-5 2.9409156805653852E-5 0.0 3.3085301406360584E-4 8.822747041696155E-5 22-23 4.4113735208480775E-5 2.9409156805653852E-5 0.0 0.001066081934204952 9.557975961837502E-5 24-25 4.4113735208480775E-5 2.9409156805653852E-5 0.0 0.0029556202589682123 1.1028433802120195E-4 26-27 4.4113735208480775E-5 2.9409156805653852E-5 0.0 0.00526423906821204 1.1763662722261541E-4 28-29 4.4113735208480775E-5 2.9409156805653852E-5 0.0 0.015182477200918802 1.1763662722261541E-4 30-31 5.146602440989424E-5 2.9409156805653852E-5 0.0 0.044848964128622125 1.1763662722261541E-4 32-33 5.8818313611307704E-5 2.9409156805653852E-5 0.0 0.09726343384549871 1.1763662722261541E-4 34-35 5.8818313611307704E-5 2.9409156805653852E-5 0.0 0.16866886656962626 1.4704578402826925E-4 36-37 5.8818313611307704E-5 4.4113735208480775E-5 0.0 0.2786885221795773 1.4704578402826925E-4 38-39 5.8818313611307704E-5 4.4113735208480775E-5 0.0 0.45002626972931664 1.4704578402826925E-4 40-41 5.8818313611307704E-5 4.4113735208480775E-5 0.0 0.6636176233195792 1.5439807322968272E-4 42-43 5.8818313611307704E-5 4.4113735208480775E-5 0.0 0.850843667833573 1.764549408339231E-4 44-45 5.8818313611307704E-5 5.146602440989424E-5 0.0 1.0704491939905916 1.9851180843816348E-4 46-47 5.8818313611307704E-5 5.8818313611307704E-5 0.0 1.3161185853666213 2.205686760424039E-4 48-49 5.8818313611307704E-5 5.8818313611307704E-5 0.0 1.5708312924603893 3.0879614645936543E-4 50-51 6.617060281272117E-5 7.352289201413463E-5 0.0 1.8480420045104822 3.0879614645936543E-4 52-53 7.352289201413463E-5 8.087518121554809E-5 0.0 2.13141393491136 3.676144600706731E-4 54-55 7.352289201413463E-5 8.822747041696155E-5 0.0 2.411183243603545 4.2643277368198087E-4 56-57 7.352289201413463E-5 8.822747041696155E-5 0.0 2.726155312992098 4.2643277368198087E-4 58-59 7.352289201413463E-5 1.1763662722261541E-4 0.0 3.0687793420671667 4.2643277368198087E-4 60-61 7.352289201413463E-5 1.3234120562544233E-4 0.0 3.4164029277991963 4.2643277368198087E-4 62-63 7.352289201413463E-5 1.3234120562544233E-4 0.0 3.76654099643811 4.2643277368198087E-4 64-65 8.087518121554809E-5 1.3234120562544233E-4 0.0 4.134221627111597 4.2643277368198087E-4 66-67 8.822747041696155E-5 1.3234120562544233E-4 0.0 4.530701174587018 4.3378506288339435E-4 68-69 8.822747041696155E-5 1.3234120562544233E-4 0.0 4.965809649526667 4.411373520848078E-4 70-71 8.822747041696155E-5 1.396934948268558E-4 0.0 5.471544214535093 4.411373520848078E-4 72-73 8.822747041696155E-5 1.4704578402826925E-4 0.0 5.946649142730432 4.999556656961155E-4 74-75 8.822747041696155E-5 1.4704578402826925E-4 0.0 6.409740430370659 4.999556656961155E-4 76-77 8.822747041696155E-5 1.4704578402826925E-4 0.0 6.909122617509064 4.999556656961155E-4 78-79 8.822747041696155E-5 1.4704578402826925E-4 0.0 7.425863559452008 4.999556656961155E-4 80-81 8.822747041696155E-5 1.4704578402826925E-4 0.0 7.98624769009454 5.220125333003559E-4 82-83 8.822747041696155E-5 1.5439807322968272E-4 0.0 8.582327184809936 5.587739793074232E-4 84-85 8.822747041696155E-5 1.6175036243109618E-4 0.0 9.157099745419634 5.587739793074232E-4 86-87 8.822747041696155E-5 1.6175036243109618E-4 0.0 9.747290056484697 5.661262685088366E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 530 0.0 33.341537 2 AGTACTT 23435 0.0 21.689955 12-13 TCACGCA 980 0.0 19.929716 4 TCTAACG 680 0.0 19.148159 2 TAACGCA 445 0.0 18.81007 4 GAGTACT 22730 0.0 18.69014 12-13 TACTTTT 27470 0.0 18.470247 14-15 ATCACGC 1035 0.0 18.421347 3 TATTCGC 290 6.663504E-8 17.637775 9 GTATAAG 2370 0.0 17.472277 1 CTAACGC 730 0.0 17.199617 3 CATGGGG 12715 0.0 17.116201 4 TAGTACT 1995 0.0 16.54982 4 GTACTTT 25915 0.0 16.411154 14-15 TACGGGC 1265 0.0 16.17484 4 GTATATA 1620 0.0 15.796306 1 GTATAAC 1125 0.0 15.715889 1 ACTTTTT 27950 0.0 15.389801 16-17 ATACGGG 1390 0.0 15.389373 3 ATGGGTA 3110 0.0 15.251678 5 >>END_MODULE