FastQCFastQC Report
Fri 27 May 2016
ERR765549_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765549_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6015515
Sequences flagged as poor quality0
Sequence length99
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA429890.7146354052811771No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT409590.6808893336646987No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG172460.28669199561467307No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG146840.24410212591939343No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT141970.23600639346755847No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG120720.2006810721941513No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC120460.20024885649857077No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC113220.18821331174471345No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC108570.180483300266062No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA106400.17687596157602464No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC95200.1582574393048642No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT89270.14839959670950867No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA84170.13992151960389093No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC83450.13872461460074492No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC80190.13330529472538927No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT77240.1284013089486104No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC73800.12268276282246823No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC72860.12112013684613869No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA66280.11018175501183192No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC64690.10753858979655109No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG61220.10177017262861117No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC60670.10085587019565241No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAG38000.034.386551
GTACAAA38950.030.8019471
GTACATA16400.029.205141
TACATGA21050.027.392742
GTACTAG43400.026.0364911
TAGGCAT52300.025.1591825
TACAAGA23900.024.3208082
CTATTGA38200.023.6149069
TACAAGG35600.022.8588282
CGTTAAC9700.022.53161
GTATAAG39850.022.4046841
ACGTTAA8200.022.1133888
GTAACAA20750.021.9621011
GTGATCG28600.021.9468548
CTAGGCA62100.021.7129174
GGGCTAT43850.021.3123326
GGCGTCG25150.021.2600568
GGCTATT45300.021.04077
AGGGCTA43250.020.8558255
CTGTGCG50950.020.6258649
Produced by FastQC (version 0.11.5)