##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765549_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6015515 Sequences flagged as poor quality 0 Sequence length 99 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.26208994574862 33.0 33.0 33.0 33.0 33.0 2 32.10303290740693 33.0 33.0 33.0 27.0 33.0 3 32.15648734979466 33.0 33.0 33.0 27.0 33.0 4 36.062185531912064 37.0 37.0 37.0 33.0 37.0 5 36.08262484591926 37.0 37.0 37.0 33.0 37.0 6 36.11572691614932 37.0 37.0 37.0 33.0 37.0 7 36.02623366411687 37.0 37.0 37.0 33.0 37.0 8 35.9893309217914 37.0 37.0 37.0 33.0 37.0 9 35.696308628604534 37.0 37.0 37.0 33.0 37.0 10-11 35.87733510763418 37.0 37.0 37.0 33.0 37.0 12-13 35.81422637962003 37.0 37.0 37.0 33.0 37.0 14-15 37.12503393308802 37.0 37.0 40.0 33.0 40.0 16-17 37.28598781650449 37.0 37.0 40.0 33.0 40.0 18-19 37.39508329710756 37.0 37.0 40.0 33.0 40.0 20-21 37.43284531748321 37.0 37.0 40.0 33.0 40.0 22-23 37.360632963262496 37.0 37.0 40.0 33.0 40.0 24-25 37.298722387027546 37.0 37.0 40.0 33.0 40.0 26-27 37.14523478039702 37.0 37.0 40.0 33.0 40.0 28-29 37.06248583870209 37.0 37.0 40.0 33.0 40.0 30-31 36.88701208458461 37.0 37.0 40.0 33.0 40.0 32-33 36.62924271654214 37.0 37.0 40.0 33.0 40.0 34-35 36.50405775731588 37.0 37.0 40.0 33.0 40.0 36-37 36.31308350157883 37.0 37.0 40.0 33.0 40.0 38-39 36.1139676320315 37.0 37.0 40.0 33.0 40.0 40-41 35.90657267083533 37.0 37.0 40.0 33.0 40.0 42-43 35.659601713236526 37.0 37.0 40.0 27.0 40.0 44-45 35.32600616904787 37.0 35.0 40.0 27.0 40.0 46-47 34.92107965818388 37.0 33.0 40.0 24.5 40.0 48-49 34.53693574033146 37.0 33.0 40.0 22.0 40.0 50-51 34.18062277294629 37.0 33.0 40.0 22.0 40.0 52-53 34.01515248486622 37.0 33.0 40.0 22.0 40.0 54-55 33.90485012505164 37.0 33.0 40.0 22.0 40.0 56-57 33.73132075973545 37.0 33.0 40.0 22.0 40.0 58-59 33.53532731611508 37.0 33.0 37.0 22.0 40.0 60-61 33.35145868641338 37.0 33.0 37.0 22.0 40.0 62-63 33.016881846358956 37.0 33.0 37.0 18.5 40.0 64-65 32.89508928163258 37.0 33.0 37.0 22.0 40.0 66-67 32.73329972579239 37.0 33.0 37.0 22.0 40.0 68-69 32.49926307223903 37.0 33.0 37.0 15.0 40.0 70-71 32.309114099125345 37.0 33.0 37.0 15.0 38.5 72-73 32.10584654846676 37.0 33.0 37.0 15.0 37.0 74-75 31.89587184139679 35.0 33.0 37.0 15.0 37.0 76-77 31.724405142369356 33.0 33.0 37.0 15.0 37.0 78-79 31.534553483783185 33.0 33.0 37.0 15.0 37.0 80-81 31.411077605159324 33.0 33.0 37.0 15.0 37.0 82-83 31.261141731007235 33.0 33.0 37.0 15.0 37.0 84-85 31.102748226876667 33.0 33.0 37.0 15.0 37.0 86-87 31.000274955677114 33.0 33.0 37.0 15.0 37.0 88-89 30.893632964093683 33.0 33.0 37.0 15.0 37.0 90-91 30.825230923703124 33.0 30.0 37.0 15.0 37.0 92-93 30.698352925726226 33.0 27.0 37.0 15.0 37.0 94-95 30.64696713415227 33.0 27.0 37.0 15.0 37.0 96-97 30.610846120406983 33.0 27.0 37.0 15.0 37.0 98-99 29.795001591717416 33.0 27.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.12490503257953378 1101 2 0.2958460251314179 1101 3 0.2573994208615673 1101 4 0.25143635016117827 1101 5 0.051575174501302 1101 6 0.15071383285444284 1101 7 0.1554126823946902 1101 8 0.165087829302486 1101 9 0.36629175788252866 1101 10-11 0.3368136785438125 1101 12-13 0.29267141555278187 1101 14-15 0.5711604411912745 1101 16-17 0.40549140602065137 1101 18-19 0.3237877082731444 1101 20-21 0.3682688085604795 1101 22-23 0.2618157494918094 1101 24-25 0.3590179780066549 1101 26-27 0.24176894459880316 1101 28-29 0.22529761283909266 1101 30-31 0.21002776024940317 1101 32-33 0.3520557978851855 1101 34-35 0.4352839014060237 1101 36-37 0.44431835748826387 1101 38-39 0.21959937592835388 1101 40-41 0.25585943531800837 1101 42-43 0.40537703716614004 1101 44-45 0.4487421198786734 1101 46-47 0.6333202927929236 1101 48-49 0.6421744408307575 1101 50-51 0.6298487769799195 1101 52-53 0.6027988560946582 1101 54-55 0.5367746507904485 1101 56-57 0.6674426608890158 1101 58-59 0.6927789743151465 1101 60-61 0.5879708334295728 1101 62-63 0.6070449023381883 1101 64-65 0.5259501391840331 1101 66-67 0.5419177916339706 1101 68-69 0.34777404939445233 1101 70-71 0.43373488151002704 1101 72-73 0.564354888198217 1101 74-75 0.4529012300021833 1101 76-77 0.4127936115214723 1101 78-79 0.6927397444784447 1101 80-81 0.6284627099296536 1101 82-83 0.5462781530650993 1101 84-85 0.6045454953140101 1101 86-87 0.4362158393708171 1101 88-89 0.5169137087041449 1101 90-91 0.5014979698894315 1101 92-93 0.6589218996692559 1101 94-95 0.6694623199719203 1101 96-97 0.5117454456143591 1101 98-99 0.6455374220736942 1102 1 0.12327413082207528 1102 2 0.30540755802968533 1102 3 0.21504361440386788 1102 4 0.18753399599703613 1102 5 0.13829129708595644 1102 6 0.21602198243628834 1102 7 0.17921603551079812 1102 8 0.14936830469729756 1102 9 0.426662981399474 1102 10-11 0.31596566121071845 1102 12-13 0.31180689601746536 1102 14-15 0.6240730890070338 1102 16-17 0.4335573819832703 1102 18-19 0.33016912751119776 1102 20-21 0.39440503658161674 1102 22-23 0.23123259463326917 1102 24-25 0.4460065785311045 1102 26-27 0.3572620429313673 1102 28-29 0.30493050726940396 1102 30-31 0.3103909972600718 1102 32-33 0.34081268267329534 1102 34-35 0.4091260739303735 1102 36-37 0.3935513305062841 1102 38-39 0.26963812842755175 1102 40-41 0.26566647573045543 1102 42-43 0.2648182371980141 1102 44-45 0.33832731424639206 1102 46-47 0.39803988004930346 1102 48-49 0.46655609485870997 1102 50-51 0.382597655797305 1102 52-53 0.44706525017794974 1102 54-55 0.3697030526437075 1102 56-57 0.5139068770222437 1102 58-59 0.43541345084010885 1102 60-61 0.35551493713128224 1102 62-63 0.46695157031059153 1102 64-65 0.32860631856838296 1102 66-67 0.38898749807967903 1102 68-69 0.4278237384803063 1102 70-71 0.34163562255805147 1102 72-73 0.42169893995418306 1102 74-75 0.3239610205282304 1102 76-77 0.2562807525164601 1102 78-79 0.48767594062654496 1102 80-81 0.4901898816298065 1102 82-83 0.48960223064795727 1102 84-85 0.484363885932634 1102 86-87 0.4585328750813886 1102 88-89 0.4684766430426528 1102 90-91 0.38831666403178744 1102 92-93 0.4526535019643596 1102 94-95 0.46347363246572115 1102 96-97 0.3263691765475656 1102 98-99 0.2715556529621921 1103 1 0.12438182426889455 1103 2 0.2706319610508032 1103 3 0.24289719340844584 1103 4 0.2050009803456021 1103 5 0.08876513053584745 1103 6 0.17842726468854408 1103 7 0.17030352260167803 1103 8 0.07601380296213023 1103 9 0.40690598537455 1103 10-11 0.2697029478613686 1103 12-13 0.3065714889906417 1103 14-15 0.4684659524355368 1103 16-17 0.41489969623152945 1103 18-19 0.24845717708880244 1103 20-21 0.33541670804301305 1103 22-23 0.2270599668678912 1103 24-25 0.24588832126788418 1103 26-27 0.1848404103407688 1103 28-29 0.0534129832382888 1103 30-31 0.10518178522799815 1103 32-33 0.24752366030833883 1103 34-35 0.27988924583446817 1103 36-37 0.28781013342009487 1103 38-39 0.13761339234730485 1103 40-41 0.02102575262794204 1103 42-43 0.15103587451342548 1103 44-45 0.17297516837684412 1103 46-47 0.21054175172608325 1103 48-49 0.2516132126189419 1103 50-51 0.2385978650859002 1103 52-53 0.25563917494144306 1103 54-55 0.22651559993837367 1103 56-57 0.31610359924011533 1103 58-59 0.30037593904551585 1103 60-61 0.23498593682619173 1103 62-63 0.2587842125086013 1103 64-65 0.0907124996034554 1103 66-67 0.13299526128613337 1103 68-69 0.19328618123243757 1103 70-71 0.11742723764962903 1103 72-73 0.1847375067687551 1103 74-75 0.1603418474638687 1103 76-77 0.09835119215722443 1103 78-79 0.267003201602396 1103 80-81 0.1569070795312406 1103 82-83 0.22654943545557416 1103 84-85 0.319990560386767 1103 86-87 0.22831702556254996 1103 88-89 0.13603600463442334 1103 90-91 0.025095586320059482 1103 92-93 0.04171151720874633 1103 94-95 0.33666809432800093 1103 96-97 0.2636965340510571 1103 98-99 0.24737733239195947 1104 1 0.1483697380336224 1104 2 0.34035874759095464 1104 3 0.2745236759481031 1104 4 0.21917132388556126 1104 5 0.1011036694926375 1104 6 0.21665626400850613 1104 7 0.1638896036537716 1104 8 0.06159557711862362 1104 9 0.4586023532284642 1104 10-11 0.2738675987609369 1104 12-13 0.2683009263596219 1104 14-15 0.4846241798840438 1104 16-17 0.3259059795297148 1104 18-19 0.25175724693703216 1104 20-21 0.34498750130821776 1104 22-23 0.31848368130291504 1104 24-25 0.3733542486234356 1104 26-27 0.21275211698427654 1104 28-29 0.13408431545495603 1104 30-31 0.21383167957137772 1104 32-33 0.3286208233253305 1104 34-35 0.2936961451396769 1104 36-37 0.20477838071440857 1104 38-39 0.12279992877260071 1104 40-41 0.1534045180238408 1104 42-43 0.21724922415926073 1104 44-45 0.25227087028596173 1104 46-47 0.39016510611848076 1104 48-49 0.43047573378449755 1104 50-51 0.2673510118694935 1104 52-53 0.32805401543721047 1104 54-55 0.2657318972632581 1104 56-57 0.3112965295455439 1104 58-59 0.3112255321077768 1104 60-61 0.33360260001280295 1104 62-63 0.35695230808791223 1104 64-65 0.2782967553404063 1104 66-67 0.28437411729646556 1104 68-69 0.21987318658981536 1104 70-71 0.2985158265251826 1104 72-73 0.2624104686190307 1104 74-75 0.23814480014429762 1104 76-77 0.19976373382274915 1104 78-79 0.34465532831275425 1104 80-81 0.3400933498182255 1104 82-83 0.27823551053643314 1104 84-85 0.24161519403060439 1104 86-87 0.25329876242735594 1104 88-89 0.17713744830399847 1104 90-91 0.20481478473315562 1104 92-93 0.13914880213020098 1104 94-95 0.2938035556838443 1104 96-97 0.21101876336015835 1104 98-99 0.19431000520025066 1105 1 0.06753509155761606 1105 2 0.25156634902077 1105 3 0.1875547672591651 1105 4 0.1443088241087409 1105 5 -0.10307828445430545 1105 6 0.14716893229478245 1105 7 0.10824018764821375 1105 8 0.00821816201344916 1105 9 0.33473731372762217 1105 10-11 0.24093057324152056 1105 12-13 0.24850572707274665 1105 14-15 0.3955165593004395 1105 16-17 0.34807861205663926 1105 18-19 0.23633937684837036 1105 20-21 0.2332121613937943 1105 22-23 0.14381901994228485 1105 24-25 0.2169578930418865 1105 26-27 0.13268793401047674 1105 28-29 0.10597134237188044 1105 30-31 0.12201902948541488 1105 32-33 0.15432388397835695 1105 34-35 0.2023932463357454 1105 36-37 0.21739088355265324 1105 38-39 0.11448085795880303 1105 40-41 0.017062461440971788 1105 42-43 0.19080888963248555 1105 44-45 0.16100216133306589 1105 46-47 0.28875252776809646 1105 48-49 0.3019834613451806 1105 50-51 0.2532189068655626 1105 52-53 0.14760597601834746 1105 54-55 0.18434392672514832 1105 56-57 0.2593000759421713 1105 58-59 0.2994860155854582 1105 60-61 0.24031207433601764 1105 62-63 0.1585500865281375 1105 64-65 0.0751419458449476 1105 66-67 0.16649400771320444 1105 68-69 0.19008361378860883 1105 70-71 0.19581056566293142 1105 72-73 0.251948047027831 1105 74-75 0.1702107346293502 1105 76-77 0.1978757552921948 1105 78-79 0.3401036729397262 1105 80-81 0.257244598614804 1105 82-83 0.1996101874622056 1105 84-85 0.14842078787349422 1105 86-87 0.15542893503866395 1105 88-89 0.1544999226078616 1105 90-91 0.14846469879347168 1105 92-93 0.12891568390081076 1105 94-95 0.28577338093187166 1105 96-97 0.10642964871698268 1105 98-99 0.13179050903946887 1106 1 0.0979667761644265 1106 2 0.27822467337399814 1106 3 0.18817562254859865 1106 4 0.16625279934309845 1106 5 -0.005950823108932468 1106 6 0.12743902125578188 1106 7 0.11880473463835273 1106 8 0.052452931577505524 1106 9 0.3177388694563419 1106 10-11 0.1850141943553254 1106 12-13 0.16975335386834445 1106 14-15 0.3785159607294091 1106 16-17 0.277611142909457 1106 18-19 0.17712332884408966 1106 20-21 0.21817197559586532 1106 22-23 0.10300655243051438 1106 24-25 0.22316067797235206 1106 26-27 0.03281399529265627 1106 28-29 0.027353346509990217 1106 30-31 0.08401157607156051 1106 32-33 0.09003466854189668 1106 34-35 0.2233101533728501 1106 36-37 0.06928298417559375 1106 38-39 0.004743353592296273 1106 40-41 -0.027091278655042572 1106 42-43 0.05822494375220799 1106 44-45 0.051654562056910436 1106 46-47 0.16268380977566466 1106 48-49 0.22497076626523693 1106 50-51 0.02743244846949011 1106 52-53 0.08099878037921115 1106 54-55 0.1417928698210531 1106 56-57 0.15808245645730068 1106 58-59 0.05750610292493974 1106 60-61 0.09212175562011282 1106 62-63 0.15180142983827238 1106 64-65 -0.038879974270841444 1106 66-67 0.04926449742028893 1106 68-69 -0.007950807751790023 1106 70-71 0.012873499939637156 1106 72-73 0.14827464164977755 1106 74-75 0.10105826858157485 1106 76-77 0.11775167225946248 1106 78-79 0.20976844536691175 1106 80-81 0.11895551104645818 1106 82-83 0.035023701824670184 1106 84-85 0.12374356220387739 1106 86-87 0.052412498426498644 1106 88-89 -0.07236220313467712 1106 90-91 -0.07795658309363773 1106 92-93 -0.14166501338810988 1106 94-95 0.12273393732495563 1106 96-97 0.09121443185544109 1106 98-99 0.00368044146394908 1107 1 0.11819252631187283 1107 2 0.22678602036770457 1107 3 0.20409945294967002 1107 4 0.19113769197990393 1107 5 0.039800818549245776 1107 6 0.1344163929730584 1107 7 0.06890182455899208 1107 8 -0.028999304763495104 1107 9 0.31234152531653336 1107 10-11 0.2175055005019786 1107 12-13 0.18322949240933184 1107 14-15 0.28233822037966405 1107 16-17 0.2687810894315632 1107 18-19 0.12112148342460216 1107 20-21 0.2141432968832646 1107 22-23 0.1023189271463707 1107 24-25 0.2103243251044873 1107 26-27 -0.014973436804773144 1107 28-29 0.05013619101556799 1107 30-31 0.02177936334453534 1107 32-33 -0.09691255485339667 1107 34-35 0.10323939385949643 1107 36-37 0.08809536667850892 1107 38-39 0.029758634279559715 1107 40-41 -0.00546530857404548 1107 42-43 0.04420347427374338 1107 44-45 -0.06651092485192578 1107 46-47 -0.0879225536261643 1107 48-49 0.010037297068457463 1107 50-51 -0.12343627665847379 1107 52-53 -0.09205753651470872 1107 54-55 -0.027370381638341712 1107 56-57 0.04728897573568247 1107 58-59 0.02585834375759788 1107 60-61 0.024683438698659188 1107 62-63 -0.03408730125300252 1107 64-65 -0.15612071438879127 1107 66-67 -0.13799276931393223 1107 68-69 -0.14778156116189933 1107 70-71 -0.08541706063586929 1107 72-73 -0.022482022095431375 1107 74-75 -0.19268917664325613 1107 76-77 -0.06762761042399745 1107 78-79 -0.051681839695774556 1107 80-81 -0.07510238379849099 1107 82-83 -0.11952639366269935 1107 84-85 -0.10367684674523403 1107 86-87 -0.07956057348143375 1107 88-89 -0.14715718912813358 1107 90-91 -0.1703441205081866 1107 92-93 -0.10524395800046804 1107 94-95 0.06265931475454423 1107 96-97 0.013238241513604976 1107 98-99 -0.03658098130120635 1108 1 0.14623965989779464 1108 2 0.23606753594879137 1108 3 0.13164084371299367 1108 4 0.11405066129022856 1108 5 -0.03469587231216309 1108 6 0.0897890873876932 1108 7 0.09219450902574522 1108 8 0.0347756534832655 1108 9 0.33748124609349617 1108 10-11 0.1480273761644142 1108 12-13 0.1966213984196017 1108 14-15 0.22564488520804815 1108 16-17 0.2825202319992357 1108 18-19 0.02880543993478568 1108 20-21 0.24650540595905568 1108 22-23 0.15439232069775244 1108 24-25 0.1265783196374528 1108 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-0.31526878572346106 2216 7 -0.39171684929577566 2216 8 -0.3100278336563349 2216 9 -0.05175183588515608 2216 10-11 -0.34832377883898147 2216 12-13 -0.24696740213661883 2216 14-15 -0.714092359367605 2216 16-17 -0.813603264430931 2216 18-19 -0.8105538836545492 2216 20-21 -1.089577442355818 2216 22-23 -0.9719249460602626 2216 24-25 -0.7513153832051742 2216 26-27 -0.9205248693270036 2216 28-29 -0.999336309714451 2216 30-31 -0.6393932730916418 2216 32-33 -0.7730841808615665 2216 34-35 -0.7954949171689236 2216 36-37 -0.7991440409807211 2216 38-39 -0.856202491602879 2216 40-41 -0.7958051568606024 2216 42-43 -0.9069124596926059 2216 44-45 -0.7474627600060089 2216 46-47 -1.1029771272982813 2216 48-49 -1.309541624070384 2216 50-51 -0.9884099198808016 2216 52-53 -0.9630341041588011 2216 54-55 -0.8642134778901251 2216 56-57 -0.9277119417686279 2216 58-59 -0.7843134010802757 2216 60-61 -0.8617404519494656 2216 62-63 -0.9643113454786842 2216 64-65 -1.011566797002093 2216 66-67 -1.0176735137191955 2216 68-69 -0.9146015797166029 2216 70-71 -0.9858360069448295 2216 72-73 -0.930915820194361 2216 74-75 -0.9315791968339795 2216 76-77 -0.8770869988207934 2216 78-79 -0.802731197109555 2216 80-81 -0.7077612216269991 2216 82-83 -0.6970349806582057 2216 84-85 -0.8339875505428402 2216 86-87 -0.9468441863102015 2216 88-89 -0.7383837384456093 2216 90-91 -0.7153262061780268 2216 92-93 -0.730595941748021 2216 94-95 -0.8401669657318251 2216 96-97 -0.8805580601884664 2216 98-99 -0.8251601716841463 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 8.0 9 33.0 10 112.0 11 207.0 12 388.0 13 756.0 14 2511.0 15 6620.0 16 14094.0 17 25342.0 18 40425.0 19 54564.0 20 68114.0 21 84218.0 22 96472.0 23 84717.0 24 60068.0 25 47979.0 26 52655.0 27 67319.0 28 88683.0 29 117686.0 30 154066.0 31 203481.0 32 269423.0 33 354684.0 34 471853.0 35 659675.0 36 1038897.0 37 1421192.0 38 528548.0 39 725.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.87076029899191 20.975557665755336 13.768088822666538 23.385593212586215 2 14.610759787239491 22.24793375789232 39.01484382611147 24.126462628756716 3 17.243793756644276 29.428502796518668 30.783764981053157 22.543938465783896 4 12.009029983301513 18.933557642196885 38.60351108757937 30.453901286922235 5 11.817169265551751 39.00541942347974 34.39390564218506 14.783505668783455 6 27.67882716608636 39.58721738704001 18.35787958304484 14.376075863828783 7 23.323239988596157 34.147483631908486 24.346045184826238 18.18323119466912 8 26.581780612300026 32.83537652220965 20.775544570996832 19.80729829449349 9 26.982315451175687 13.56816199357267 21.753506212791454 37.69601634246019 10-11 24.355487435406612 26.321378967553066 27.555479456039922 21.7676541410004 12-13 26.367900337710072 24.676756686667726 27.779982262532798 21.175360713089404 14-15 23.888847651900786 24.017351896572205 27.674976690942554 24.418823760584456 16-17 21.030645750787773 26.482200165463098 29.067193262578034 23.4199608211711 18-19 21.49961391501808 24.985067778901723 33.00750642297459 20.507811883105603 20-21 22.819542466438865 24.272418903452156 32.13301770505102 20.775020925057955 22-23 22.252242742308848 24.270906148517625 31.202989270245357 22.273861838928173 24-25 21.551646035293736 25.186413798319844 31.423419274991417 21.838520891395003 26-27 22.087701551737464 24.31308873803822 31.40189992045569 22.197309789768624 28-29 21.2641311674894 24.281071529204066 32.46890748339918 21.985889819907356 30-31 22.339916033789294 24.2759265000586 31.906395379281744 21.477762086870367 32-33 21.46990739778722 24.7904044790845 31.32801597203232 22.411672151095956 34-35 21.534423902192913 25.017101611416482 32.05561784818091 21.392856638209697 36-37 22.45264121193281 24.386490599724215 31.099689719001617 22.06117846934136 38-39 22.575772814131458 24.02439358891134 31.156925051304835 22.24290854565237 40-41 21.87957307063485 24.28008242020841 31.522404981119656 22.317939528037083 42-43 22.96272222744021 24.270207953932456 30.362346366021864 22.40472345260547 44-45 22.779780707897153 24.764738714244928 29.81300367343156 22.642476904426363 46-47 22.41625197510105 24.85404824025873 29.739631602614242 22.990068182025976 48-49 23.59422260604454 25.20159121870696 28.5428263415518 22.661359833696697 50-51 22.374227310546146 25.99009394873091 27.641897659635127 23.99378108108782 52-53 21.898980734122784 26.352247009644742 26.918766268373766 24.830005987858712 54-55 22.316875612478732 26.424395916226622 26.7575677227968 24.501160748497846 56-57 23.206420717801592 25.26171503464113 26.679801701098892 24.85206254645838 58-59 22.175981607559788 25.399371458636544 28.19013002211781 24.234516911685866 60-61 22.73319075756606 25.935767760532556 27.362262416434834 23.968779065466546 62-63 22.003693781829153 25.61454006847294 27.294820144243676 25.08694600545423 64-65 21.294560814826326 25.75317325283039 27.645338761519174 25.30692717082411 66-67 22.939224654913172 25.245195132918795 27.18959224605042 24.625987966117613 68-69 22.672184476239895 24.70882175431999 27.458275845760333 25.160717923679776 70-71 21.957656741256717 25.034041209853942 27.600102601561332 25.408199447328013 72-73 23.08454055887152 24.733559803275366 27.651040683964716 24.530858953888405 74-75 23.159554917575637 24.894884311650788 27.439828510110935 24.505732260662636 76-77 22.146682370503605 25.364046137363133 27.78464520494089 24.70462628719237 78-79 22.366323289406477 25.623712439172685 27.458990265551826 24.550974005869012 80-81 21.68229070257168 25.727348237197646 27.718607598798062 24.871753461432615 82-83 21.990162105821366 25.38652135353332 27.27543693266495 25.34787960798036 84-85 22.267420162695963 25.772598023610616 26.369429716325204 25.590552097368224 86-87 21.6766311778792 25.344106032484337 27.084073433446683 25.895189356189785 88-89 22.083346147420464 24.531249610382485 27.36565364727708 26.019750594919973 90-91 22.240963645121326 24.915565932465213 27.125742405962605 25.71772801645086 92-93 21.79894410341094 25.11815188090879 27.04536222645779 26.03754178922248 94-95 20.601300611738687 25.270556266776516 27.846187426200782 26.281955695284015 96-97 21.823695301828998 25.014576925062176 26.979991107971813 26.181736665137006 98-99 21.565668109879205 25.16878438504434 26.88863713248159 26.376910372594864 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 131.0 1 100.5 2 155.5 3 235.5 4 190.5 5 205.0 6 386.0 7 877.0 8 1580.5 9 1689.5 10 1186.0 11 912.0 12 987.5 13 1241.5 14 1623.5 15 2348.5 16 3185.5 17 4037.0 18 5319.0 19 6836.0 20 8671.5 21 10979.5 22 13517.5 23 16459.0 24 19845.0 25 24370.5 26 30396.0 27 36040.0 28 43880.0 29 55919.0 30 65198.5 31 76787.5 32 92467.5 33 105919.0 34 117114.0 35 128736.0 36 134996.5 37 147035.0 38 162516.5 39 152447.5 40 140111.5 41 142913.0 42 147742.5 43 160207.0 44 185649.5 45 220946.5 46 247971.5 47 277718.0 48 301245.5 49 326725.5 50 370159.0 51 327914.5 52 248754.5 53 223135.5 54 194794.5 55 176558.0 56 158610.5 57 129172.5 58 121823.0 59 105784.0 60 78270.0 61 57899.5 62 45484.5 63 36650.5 64 25263.5 65 17623.0 66 12263.5 67 8962.0 68 7254.5 69 6446.5 70 6045.5 71 5714.5 72 6271.5 73 5350.5 74 3315.0 75 2568.0 76 2027.5 77 1298.0 78 667.0 79 441.5 80 347.0 81 256.0 82 225.0 83 186.5 84 125.5 85 86.5 86 39.5 87 14.5 88 6.0 89 6.0 90 5.0 91 3.0 92 2.0 93 0.5 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.057285203345017015 2 0.008128979813033463 3 0.0 4 0.0 5 0.0019117232689138005 6 0.0 7 0.0 8 0.0 9 0.0043886516782021155 10-11 0.0 12-13 0.0 14-15 0.0024852402495879403 16-17 0.0045964476856927465 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 3.7403281348313486E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 1.4961312539325393E-4 54-55 0.0 56-57 8.394958702621472E-4 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.001404701010636662 70-71 1.9948416719100525E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 3.7403281348313486E-4 80-81 8.394958702621472E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 3.7403281348313486E-4 92-93 9.724853150561506E-4 94-95 6.316998627715167E-4 96-97 2.4104336868913137E-4 98-99 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 6015515.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.15959520678679 #Duplication Level Percentage of deduplicated Percentage of total 1 72.20340880633502 18.166085381175336 2 14.763332273923558 7.428789278304157 3 5.220239773178722 3.940173587266354 4 2.3973800189586156 2.4126844333535042 5 1.2695415711399687 1.5970576014034863 6 0.7621701371455948 1.15055352775706 7 0.493384545744539 0.8689348816551886 8 0.33013646468557817 0.6644879851591047 9 0.23971749860766375 0.5428075706057067 >10 1.6398586175808996 8.37608293851006 >50 0.27608609575342924 4.9366272859810065 >100 0.31037012335864156 16.83984792273808 >500 0.054552654098758056 9.500023295414032 >1k 0.03739984668244664 16.65130654456264 >5k 0.0014798500485860182 2.4882770235440863 >10k+ 9.417227581911025E-4 4.436260742570361 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 46188 0.7678145595181792 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 43598 0.7247592267661206 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23727 0.39443006957841514 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 18040 0.2998911980104779 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17641 0.29325834945137697 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 15855 0.2635684559011157 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 14384 0.2391150217396183 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 13449 0.22357188037931916 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 12897 0.2143956086885329 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 12248 0.20360683997961937 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11856 0.1970903571847132 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 11776 0.19576046273677314 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 10292 0.1710909207274855 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 10180 0.16922906850036945 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 9395 0.15617947922995787 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 8844 0.14701983121977086 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 8291 0.13782693584838537 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 8252 0.1371786123050146 No Hit GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC 7922 0.13169279770726197 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 7849 0.1304792690235167 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 7769 0.12914937457557665 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 6887 0.11448728828703776 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 6885 0.11445404092583927 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 6828 0.11350649113168199 No Hit GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT 6325 0.10514477979025902 No Hit CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA 6318 0.10502841402606426 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 6231 0.10358215381392948 No Hit GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA 6074 0.10097223595984717 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.662368059925044E-5 0.0 3 0.0 0.0 0.0 1.662368059925044E-5 0.0 4 0.0 0.0 0.0 1.662368059925044E-5 0.0 5 0.0 0.0 0.0 3.324736119850088E-5 0.0 6 0.0 0.0 0.0 3.324736119850088E-5 0.0 7 0.0 0.0 0.0 3.324736119850088E-5 0.0 8 0.0 0.0 0.0 3.324736119850088E-5 0.0 9 0.0 0.0 0.0 4.987104179775131E-5 0.0 10-11 0.0 0.0 0.0 9.14302432958774E-5 0.0 12-13 0.0 0.0 0.0 1.329894447940035E-4 0.0 14-15 0.0 0.0 0.0 1.828604865917548E-4 0.0 16-17 1.662368059925044E-5 0.0 0.0 2.4935520898875657E-4 8.31184029962522E-6 18-19 1.662368059925044E-5 0.0 0.0 3.490972925842592E-4 1.662368059925044E-5 20-21 1.662368059925044E-5 0.0 0.0 7.480656269662697E-4 1.662368059925044E-5 22-23 4.15592014981261E-5 0.0 0.0 0.0026764125764793204 1.662368059925044E-5 24-25 7.480656269662697E-5 0.0 0.0 0.007139870817378063 1.662368059925044E-5 26-27 1.329894447940035E-4 0.0 0.0 0.011869307947864813 1.662368059925044E-5 28-29 1.4961312539325393E-4 0.0 0.0 0.02691373889018646 1.662368059925044E-5 30-31 1.5792496569287917E-4 0.0 0.0 0.07150676209767576 1.662368059925044E-5 32-33 1.6623680599250437E-4 0.0 0.0 0.14697827201827274 1.662368059925044E-5 34-35 1.6623680599250437E-4 0.0 0.0 0.24484187970606008 1.662368059925044E-5 36-37 1.6623680599250437E-4 0.0 0.0 0.39564359826216045 1.662368059925044E-5 38-39 1.6623680599250437E-4 0.0 0.0 0.6318744114178088 1.662368059925044E-5 40-41 1.7454864629212958E-4 0.0 0.0 0.9236449414555528 1.662368059925044E-5 42-43 2.0779600749063046E-4 0.0 0.0 1.1780038783046838 1.662368059925044E-5 44-45 2.161078477902557E-4 0.0 0.0 1.4590438225156117 1.662368059925044E-5 46-47 2.3273152838950613E-4 0.0 0.0 1.759059698130584 1.662368059925044E-5 48-49 2.3273152838950613E-4 0.0 0.0 2.083961223602634 1.662368059925044E-5 50-51 2.3273152838950613E-4 0.0 0.0 2.463779077934308 1.662368059925044E-5 52-53 2.3273152838950613E-4 0.0 0.0 2.8347115749856826 1.662368059925044E-5 54-55 2.3273152838950613E-4 8.31184029962522E-6 0.0 3.169994588991965 1.662368059925044E-5 56-57 2.3273152838950613E-4 1.662368059925044E-5 0.0 3.552630157185212 1.662368059925044E-5 58-59 2.4935520898875657E-4 1.662368059925044E-5 0.0 3.9770909057661727 1.662368059925044E-5 60-61 2.4935520898875657E-4 1.662368059925044E-5 0.0 4.393065265401217 1.662368059925044E-5 62-63 2.4935520898875657E-4 1.662368059925044E-5 0.0 4.811076025909669 1.662368059925044E-5 64-65 2.4935520898875657E-4 1.662368059925044E-5 0.0 5.254138673081191 1.662368059925044E-5 66-67 2.4935520898875657E-4 1.662368059925044E-5 0.0 5.727489666304548 1.662368059925044E-5 68-69 2.576670492883818E-4 1.662368059925044E-5 0.0 6.233639181350226 1.662368059925044E-5 70-71 2.6597888958800704E-4 1.662368059925044E-5 0.0 6.827653160203241 1.662368059925044E-5 72-73 2.6597888958800704E-4 3.324736119850088E-5 0.0 7.376226308138206 1.662368059925044E-5 74-75 2.6597888958800704E-4 3.324736119850088E-5 0.0 7.900753302086355 1.662368059925044E-5 76-77 2.6597888958800704E-4 3.324736119850088E-5 0.0 8.456142159067012 1.662368059925044E-5 78-79 2.6597888958800704E-4 3.324736119850088E-5 0.0 9.011688941013363 1.662368059925044E-5 80-81 2.6597888958800704E-4 3.324736119850088E-5 0.0 9.619326026117465 1.662368059925044E-5 82-83 2.6597888958800704E-4 3.324736119850088E-5 0.0 10.291097270973474 1.662368059925044E-5 84-85 2.6597888958800704E-4 3.324736119850088E-5 0.0 10.920253710613306 1.662368059925044E-5 86-87 2.6597888958800704E-4 3.324736119850088E-5 0.0 11.546592436391563 1.662368059925044E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 480 0.0 55.222736 2 TCACGCA 630 0.0 42.075165 4 ATCACGC 1065 0.0 24.889534 3 TAGGCAT 4970 0.0 24.328115 5 AGTACTT 45340 0.0 22.995646 12-13 TTCTGGC 3015 0.0 22.828466 9 CTATTGA 4000 0.0 21.97375 9 AGGGCTA 4120 0.0 21.671827 5 TATTCGC 410 0.0 21.55123 9 CTGTGCG 4845 0.0 21.500896 9 TAGTACT 5090 0.0 21.01365 4 TAACGCA 690 0.0 20.893158 4 CTAGGCA 5745 0.0 20.88436 4 GGGCTAT 4480 0.0 20.656109 6 GTATCAA 92285 0.0 20.259912 1 ACAACGC 670 0.0 20.128653 3 CCGAGTA 625 0.0 20.089783 8 ACTGTGC 5675 0.0 20.076672 8 GAGTACT 43765 0.0 19.976637 12-13 GGCTATT 4555 0.0 19.703455 7 >>END_MODULE