FastQCFastQC Report
Fri 27 May 2016
ERR765547_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765547_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6960671
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA359180.516013470540412No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT319550.4590793042797167No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG142160.20423318384104064No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG119620.17185124824891163No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG102270.14692549037298272No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC101210.1454026486814274No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT99000.1422276674188451No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC92490.13287512080372713No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC91520.13148157699164348No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA85010.12212903037652548No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAG38100.026.4841291
GTACATA22200.026.182311
GTACTAG25150.025.6996861
GTACAAA44600.021.0604441
GTATAAG28750.020.3790361
TCTAACG12900.020.1856652
CTAACGC13200.020.0791653
TAGGCAT36850.019.8110245
CTAGGCA41500.019.3839684
TAGTACT30550.019.1780344
GTGATCG21350.018.7303268
GTATAAT20550.018.1021081
TGATCGC22000.017.9655699
TACATGA28450.017.8151282
CTAGTAC34850.017.345443
TACATAG26450.016.8768082
GTATATA16550.016.857931
ACGCCTA15500.016.7996836
CAACACA97100.016.7607565
TAAGGTG28400.016.536664
Produced by FastQC (version 0.11.5)