##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765547_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6960671 Sequences flagged as poor quality 0 Sequence length 99 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.295957674195492 33.0 33.0 33.0 27.0 33.0 2 31.16011789667979 33.0 33.0 33.0 27.0 33.0 3 31.182062620112344 33.0 33.0 33.0 27.0 33.0 4 34.72184693113638 37.0 37.0 37.0 27.0 37.0 5 34.958192536323004 37.0 37.0 37.0 33.0 37.0 6 34.79798168883431 37.0 37.0 37.0 33.0 37.0 7 34.725112995571834 37.0 37.0 37.0 33.0 37.0 8 34.5951564152364 37.0 37.0 37.0 27.0 37.0 9 34.45642007789191 37.0 37.0 37.0 27.0 37.0 10-11 34.53728943660748 37.0 37.0 37.0 27.0 37.0 12-13 34.48384509194588 37.0 37.0 37.0 27.0 37.0 14-15 35.94028786879886 37.0 37.0 40.0 27.0 40.0 16-17 36.034402214958874 37.0 37.0 40.0 27.0 40.0 18-19 36.05788457750697 37.0 37.0 40.0 27.0 40.0 20-21 36.08553449803905 37.0 37.0 40.0 27.0 40.0 22-23 36.18670290551012 37.0 37.0 40.0 27.0 40.0 24-25 36.19282508827094 37.0 37.0 40.0 27.0 40.0 26-27 36.22502615049612 37.0 37.0 40.0 27.0 40.0 28-29 36.164508206177246 37.0 37.0 40.0 27.0 40.0 30-31 36.121803558881034 37.0 37.0 40.0 27.0 40.0 32-33 35.97469144856868 37.0 37.0 40.0 27.0 40.0 34-35 35.91159781865858 37.0 37.0 40.0 27.0 40.0 36-37 35.79016800248137 37.0 37.0 40.0 27.0 40.0 38-39 35.56163306669716 37.0 37.0 40.0 27.0 40.0 40-41 35.456785703562204 37.0 33.0 40.0 27.0 40.0 42-43 35.30747057288011 37.0 33.0 40.0 27.0 40.0 44-45 35.15943994479842 37.0 33.0 40.0 27.0 40.0 46-47 35.033215332257484 37.0 33.0 40.0 27.0 40.0 48-49 34.8641807664807 37.0 33.0 40.0 27.0 40.0 50-51 34.696385233550046 37.0 33.0 40.0 27.0 40.0 52-53 34.510690923331964 37.0 33.0 40.0 27.0 40.0 54-55 34.37023499602265 37.0 33.0 40.0 27.0 40.0 56-57 34.16822049196119 37.0 33.0 40.0 24.5 40.0 58-59 33.93263609212387 37.0 33.0 37.0 22.0 40.0 60-61 33.686263651880694 37.0 33.0 37.0 22.0 40.0 62-63 33.47601567435093 37.0 33.0 37.0 22.0 40.0 64-65 33.166410393480746 37.0 33.0 37.0 22.0 40.0 66-67 32.93168855416381 37.0 33.0 37.0 22.0 40.0 68-69 32.61425328104144 37.0 33.0 37.0 22.0 38.5 70-71 32.43666673801994 37.0 33.0 37.0 22.0 37.0 72-73 32.13585787921883 33.0 33.0 37.0 22.0 37.0 74-75 31.850681708703085 33.0 33.0 37.0 22.0 37.0 76-77 31.64136539422708 33.0 33.0 37.0 22.0 37.0 78-79 31.40053660056624 33.0 30.0 37.0 22.0 37.0 80-81 31.14198171411923 33.0 27.0 37.0 18.5 37.0 82-83 30.952898937473126 33.0 27.0 37.0 15.0 37.0 84-85 30.75220118864977 33.0 27.0 37.0 15.0 37.0 86-87 30.528119918323966 33.0 27.0 37.0 15.0 37.0 88-89 30.33805067068965 33.0 27.0 37.0 15.0 37.0 90-91 30.12958132915634 33.0 27.0 37.0 15.0 37.0 92-93 29.956131887285004 33.0 27.0 37.0 15.0 37.0 94-95 29.721982550245514 33.0 27.0 37.0 15.0 37.0 96-97 29.63010060380673 33.0 27.0 37.0 15.0 37.0 98-99 28.467348765657796 33.0 24.5 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.36250557276612483 1101 2 0.3468941007403892 1101 3 0.40205226644667746 1101 4 0.439648805567721 1101 5 0.3996820739491511 1101 6 0.4106523489762637 1101 7 0.3227078774355334 1101 8 0.4118576526739517 1101 9 0.3566742771850713 1101 10-11 0.3536463097945344 1101 12-13 0.2845516794146761 1101 14-15 0.43192418458684756 1101 16-17 0.43551292154408827 1101 18-19 0.4060396494727243 1101 20-21 0.36677303707991626 1101 22-23 0.32944279184933123 1101 24-25 0.3766281300563463 1101 26-27 0.3772983908631886 1101 28-29 0.3284843287688304 1101 30-31 0.36814141459285565 1101 32-33 0.45969567584531035 1101 34-35 0.431944399110165 1101 36-37 0.43086288091058833 1101 38-39 0.3777584324115679 1101 40-41 0.35489110800491375 1101 42-43 0.39128382840155496 1101 44-45 0.38044142030982897 1101 46-47 0.3700600949647779 1101 48-49 0.428501569309681 1101 50-51 0.38355934883849585 1101 52-53 0.3368159078213111 1101 54-55 0.4095263916314096 1101 56-57 0.4015352916994601 1101 58-59 0.35230388528907497 1101 60-61 0.4918477258134786 1101 62-63 0.4791156669775063 1101 64-65 0.3385231346705666 1101 66-67 0.4201084667702446 1101 68-69 0.4007795737983031 1101 70-71 0.39681626660512137 1101 72-73 0.4621103844800629 1101 74-75 0.33985559289064327 1101 76-77 0.39189832394063373 1101 78-79 0.4461406310306337 1101 80-81 0.35739254742031434 1101 82-83 0.3714515455063925 1101 84-85 0.33148667988713143 1101 86-87 0.2459936212462921 1101 88-89 0.33951603449425605 1101 90-91 0.3594305697762543 1101 92-93 0.37073209663573437 1101 94-95 0.3941158591279539 1101 96-97 0.2977081519897702 1101 98-99 0.2570375296546068 1102 1 0.315528588450519 1102 2 0.3360962254716746 1102 3 0.3742646420038547 1102 4 0.4241363918323344 1102 5 0.3169670664231674 1102 6 0.3406791498082171 1102 7 0.23226715413532162 1102 8 0.3650771540835933 1102 9 0.3574742068261685 1102 10-11 0.30727657378810136 1102 12-13 0.3437227513450125 1102 14-15 0.39899357985392214 1102 16-17 0.42653623173328015 1102 18-19 0.4000341908396905 1102 20-21 0.3946058666668151 1102 22-23 0.35350552360961274 1102 24-25 0.4065116395330861 1102 26-27 0.41639311980911486 1102 28-29 0.2286629226561061 1102 30-31 0.3203078823121288 1102 32-33 0.47074995603395564 1102 34-35 0.4081130080315134 1102 36-37 0.49356012191979204 1102 38-39 0.47479166580124854 1102 40-41 0.40685626737095504 1102 42-43 0.389625952121321 1102 44-45 0.3057719213842347 1102 46-47 0.3960035956958876 1102 48-49 0.4192666283292539 1102 50-51 0.3622423260582366 1102 52-53 0.35931033438510696 1102 54-55 0.4503387482113794 1102 56-57 0.3869722419366539 1102 58-59 0.335840764854062 1102 60-61 0.43432334077128587 1102 62-63 0.30433238312366484 1102 64-65 0.22193045226445918 1102 66-67 0.34453038920626966 1102 68-69 0.2978889174330419 1102 70-71 0.34386381122728693 1102 72-73 0.2997445844541957 1102 74-75 0.337784100731497 1102 76-77 0.31222532049742924 1102 78-79 0.4155444691711523 1102 80-81 0.3359861213971911 1102 82-83 0.35099565834201485 1102 84-85 0.2756007015829205 1102 86-87 0.3464517037694037 1102 88-89 0.41275715157155446 1102 90-91 0.37166963874722114 1102 92-93 0.3720490880856957 1102 94-95 0.4544985743816419 1102 96-97 0.3394060930073479 1102 98-99 0.32763487311389383 1103 1 0.17645765821271198 1103 2 0.2777081806535584 1103 3 0.15524844104855262 1103 4 0.23893722228509517 1103 5 0.23831788277168897 1103 6 0.1868315224630379 1103 7 0.18634195565309852 1103 8 0.18211628672605684 1103 9 0.19703838536015894 1103 10-11 0.14402961574005246 1103 12-13 0.058919615995385755 1103 14-15 0.10230226279048793 1103 16-17 0.033425314709091936 1103 18-19 0.07626345938226109 1103 20-21 0.10792508071789797 1103 22-23 0.09251549848106322 1103 24-25 0.1680775127405738 1103 26-27 0.26919882716823906 1103 28-29 0.17461644887492156 1103 30-31 0.19128720238553854 1103 32-33 0.3193594786524159 1103 34-35 0.1730199266743071 1103 36-37 0.2509852860725985 1103 38-39 0.21262161035638627 1103 40-41 0.19384149988461985 1103 42-43 0.13250119334927035 1103 44-45 0.052002152655184375 1103 46-47 0.08686221179559084 1103 48-49 0.08935930621024823 1103 50-51 0.0959807942805071 1103 52-53 0.1229018086446203 1103 54-55 0.12675509008794705 1103 56-57 0.12749616543403874 1103 58-59 0.14192220642705422 1103 60-61 0.15318600884371136 1103 62-63 0.06624724766079737 1103 64-65 0.08978073539687159 1103 66-67 0.09106317718067203 1103 68-69 0.10155501075615803 1103 70-71 -0.023146341401208304 1103 72-73 0.05029870830220062 1103 74-75 0.07888786303436035 1103 76-77 0.10889584449945744 1103 78-79 -0.002667370116366641 1103 80-81 0.007745299160003327 1103 82-83 0.08835315591904802 1103 84-85 0.043202447770728014 1103 86-87 0.17053123489444744 1103 88-89 0.12344213290247552 1103 90-91 0.06555734472972219 1103 92-93 0.09790991715794206 1103 94-95 0.02599002301611364 1103 96-97 -0.027790073348445077 1103 98-99 0.0685657727388822 1104 1 0.19439241247039973 1104 2 0.1798104299113632 1104 3 0.15831955167385203 1104 4 0.2302584420427678 1104 5 0.24396401249395439 1104 6 0.09918956237500254 1104 7 0.06398124955734374 1104 8 0.05391503197152048 1104 9 0.07600500944890598 1104 10-11 0.14461096871961843 1104 12-13 0.11719770576741695 1104 14-15 0.17407511046936008 1104 16-17 0.16481805053936682 1104 18-19 0.15838874105664758 1104 20-21 0.14395726853879154 1104 22-23 0.17649632500461365 1104 24-25 0.16566363892783187 1104 26-27 0.1796044734404063 1104 28-29 0.10614975492885037 1104 30-31 0.09051905126807469 1104 32-33 0.1476628124797088 1104 34-35 0.1166984171418406 1104 36-37 0.21164083126143396 1104 38-39 0.19987677884621036 1104 40-41 0.1558953054805272 1104 42-43 0.05460263341085181 1104 44-45 0.12320973175035732 1104 46-47 0.03464985222439765 1104 48-49 0.0499094513528604 1104 50-51 0.1455640422420572 1104 52-53 0.05789492623070913 1104 54-55 0.16016038515142128 1104 56-57 0.08673528849944745 1104 58-59 0.12146362190192406 1104 60-61 0.145472246697679 1104 62-63 0.059103602701469526 1104 64-65 0.12455905256473443 1104 66-67 0.11150529837016165 1104 68-69 0.024573494224348735 1104 70-71 0.05277925257617255 1104 72-73 0.08585596130507867 1104 74-75 -0.007032473176359133 1104 76-77 -0.09706776188634691 1104 78-79 -0.10062401175331459 1104 80-81 -0.05130123483713689 1104 82-83 0.023459001242347455 1104 84-85 -0.03632585912695774 1104 86-87 0.07438873322841388 1104 88-89 0.09050317574603639 1104 90-91 0.05463829988186575 1104 92-93 0.14722846426701608 1104 94-95 0.09065897332305539 1104 96-97 -0.14926412883441387 1104 98-99 -0.10582406869454175 1105 1 0.16115468989970694 1105 2 0.2508376424947265 1105 3 0.15291027456284212 1105 4 0.15481939279892742 1105 5 0.17102233233585196 1105 6 0.19529866784277772 1105 7 0.17907178574213134 1105 8 0.06708305381437896 1105 9 0.08578167760288835 1105 10-11 0.13496790461719144 1105 12-13 0.11391088125090931 1105 14-15 0.15831464196853062 1105 16-17 0.15978394806105456 1105 18-19 0.13607803287646902 1105 20-21 0.15900923839084413 1105 22-23 0.14934507514620776 1105 24-25 0.1765936513821984 1105 26-27 0.20757275910564488 1105 28-29 0.21902486230889906 1105 30-31 0.14568348981775614 1105 32-33 0.16009097828072072 1105 34-35 0.15043480559221933 1105 36-37 0.1638933717492037 1105 38-39 0.08390485264379777 1105 40-41 0.0055599380710660284 1105 42-43 0.08468930481052439 1105 44-45 0.06583598280119674 1105 46-47 0.07830142209668622 1105 48-49 0.1036159703918429 1105 50-51 0.189302909212671 1105 52-53 0.06407432986525663 1105 54-55 0.24366882292591896 1105 56-57 0.2193062036242921 1105 58-59 0.1941280490843269 1105 60-61 0.19771954193231522 1105 62-63 0.20038836781077407 1105 64-65 0.08380132534496454 1105 66-67 0.21278125895391042 1105 68-69 0.13650917957713204 1105 70-71 0.13610865340377387 1105 72-73 0.20173074027485427 1105 74-75 0.2120308454433939 1105 76-77 0.07329926435294354 1105 78-79 0.2113682681688296 1105 80-81 0.12326217052720168 1105 82-83 0.18964899990780282 1105 84-85 0.14526960014137202 1105 86-87 0.11293913366196051 1105 88-89 0.1499401451487401 1105 90-91 0.17299685248764263 1105 92-93 0.15259583641585195 1105 94-95 0.11354964835352988 1105 96-97 0.051818451195369875 1105 98-99 0.19241481515037506 1106 1 -0.006309894512497749 1106 2 0.09185877177809232 1106 3 0.012482443692736211 1106 4 -0.01913939442381718 1106 5 -0.0143824941871884 1106 6 -0.052301249021311946 1106 7 -0.03486130942207666 1106 8 -0.06945811287343417 1106 9 -0.04849499301037241 1106 10-11 -0.08357781422675714 1106 12-13 -0.015290398362303392 1106 14-15 -0.05197745801500275 1106 16-17 -0.0964135092471139 1106 18-19 -0.058061043129981726 1106 20-21 -0.06506592356667795 1106 22-23 -0.05538184186666939 1106 24-25 -0.03068872504557163 1106 26-27 0.03903159708242754 1106 28-29 -0.02447625261032016 1106 30-31 -0.02530374318616424 1106 32-33 0.04101222802565019 1106 34-35 -0.10618251883946783 1106 36-37 -0.13184798243438678 1106 38-39 -0.12947811552222532 1106 40-41 -0.0611724311539632 1106 42-43 -0.04425195861828968 1106 44-45 -0.03709257934234955 1106 46-47 -0.09982641879347653 1106 48-49 -0.08781776496238791 1106 50-51 -0.018830898303292543 1106 52-53 -0.12276680955760355 1106 54-55 -0.014408994355889604 1106 56-57 0.044192485857017516 1106 58-59 -0.04656003296715028 1106 60-61 -0.014368385378070059 1106 62-63 -0.05372800589920246 1106 64-65 -0.16121091278427002 1106 66-67 -0.1577765100393833 1106 68-69 -0.08792171565332296 1106 70-71 -0.11085767278645164 1106 72-73 0.024517271669729723 1106 74-75 -0.08559354555889342 1106 76-77 -0.06854413165914153 1106 78-79 -0.1036370677640086 1106 80-81 -0.1600596650351065 1106 82-83 -0.1558489539666077 1106 84-85 -0.21614308549275307 1106 86-87 -0.2570813713245954 1106 88-89 -0.07823658394342914 1106 90-91 -0.17346777816419134 1106 92-93 -0.1829772461631194 1106 94-95 -0.23717554342828606 1106 96-97 -0.33387544985787443 1106 98-99 0.10994583479316944 1107 1 0.060353298907205755 1107 2 0.07998645788009995 1107 3 -0.028744053749786502 1107 4 -0.06004582243087242 1107 5 0.016331099453573472 1107 6 -0.06498032439519363 1107 7 -0.07062100018475093 1107 8 -0.031915720833687544 1107 9 -0.17048027587753722 1107 10-11 -0.04061294344030841 1107 12-13 -0.14782018185074008 1107 14-15 -0.08415759478558016 1107 16-17 -0.05853255333620666 1107 18-19 -0.12171137900559614 1107 20-21 -0.11335384176400254 1107 22-23 -0.17242474582324263 1107 24-25 -0.028420769320767647 1107 26-27 -0.023166355735497746 1107 28-29 -0.008447089803603092 1107 30-31 -0.027410826676771194 1107 32-33 -0.11431802591219764 1107 34-35 -0.14461955114426672 1107 36-37 -0.08217337873357167 1107 38-39 -0.05490881419081006 1107 40-41 -0.15791346551218055 1107 42-43 -0.238229569420227 1107 44-45 -0.10436190155729008 1107 46-47 -0.26530146214553696 1107 48-49 -0.1980975896596604 1107 50-51 -0.18187506307614854 1107 52-53 -0.12854559111948305 1107 54-55 -0.05007948961537778 1107 56-57 -0.004512265146786376 1107 58-59 -0.02713610942683431 1107 60-61 -0.023464366741826836 1107 62-63 -0.2555120601538903 1107 64-65 -0.24656552662231235 1107 66-67 -0.05521891090241127 1107 68-69 -0.19233254876816375 1107 70-71 -0.12674268778563658 1107 72-73 -0.09880368158736275 1107 74-75 -0.14153894152324753 1107 76-77 -0.2755178048644886 1107 78-79 -0.14252480912103493 1107 80-81 -0.2708302053054936 1107 82-83 -0.22787422133747626 1107 84-85 -0.2526586478135222 1107 86-87 -0.2147799905768224 1107 88-89 -0.1936995217289308 1107 90-91 -0.1287953278736218 1107 92-93 -0.1508171234457194 1107 94-95 -0.12389314450760835 1107 96-97 -0.1797342620752751 1107 98-99 0.08159750420221101 1108 1 0.008539321938858535 1108 2 0.06182226749838193 1108 3 0.051064996513225935 1108 4 0.04249775529896738 1108 5 0.058865083191506073 1108 6 -0.1192045066096199 1108 7 -0.03289692194588412 1108 8 -0.08396410751402783 1108 9 -0.0609297910124198 1108 10-11 -0.061648028207244465 1108 12-13 -0.16329298362210665 1108 14-15 -0.08788384459070642 1108 16-17 -0.10571416942093492 1108 18-19 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0.034327221535573216 2215 80-81 0.15847537499737996 2215 82-83 -5.860577648384435E-4 2215 84-85 0.06316813970197543 2215 86-87 0.09144438394083565 2215 88-89 -0.06786181984307404 2215 90-91 0.17202795435258977 2215 92-93 0.20605272384909767 2215 94-95 0.05407357743431973 2215 96-97 0.11004111156080043 2215 98-99 0.08782721723175158 2216 1 -0.06480941159343345 2216 2 -0.08391265824612759 2216 3 0.02175679710915901 2216 4 -0.010101279576623767 2216 5 -0.05895798287868814 2216 6 0.06277091590622064 2216 7 0.024976483397182392 2216 8 0.04436203570742947 2216 9 0.052285512943960555 2216 10-11 -0.008894780103133826 2216 12-13 0.01605399458228618 2216 14-15 -0.006875519977448619 2216 16-17 -0.024610791045432734 2216 18-19 -0.0980563183586014 2216 20-21 -0.12128789894519798 2216 22-23 -0.20813584786765205 2216 24-25 -0.1296612822209937 2216 26-27 -0.19505222322478488 2216 28-29 -0.08707202256725566 2216 30-31 -0.11944529372623691 2216 32-33 -0.13608222878561094 2216 34-35 -0.18431117501589256 2216 36-37 -0.1821626582974929 2216 38-39 -0.11437276075817948 2216 40-41 -0.07694395945513577 2216 42-43 -0.09000463430182748 2216 44-45 -0.07534237041034686 2216 46-47 0.010251941588002467 2216 48-49 0.01792756985997812 2216 50-51 0.011269089417424993 2216 52-53 0.10816117218254107 2216 54-55 -0.0468523266090628 2216 56-57 0.12646268776648384 2216 58-59 -0.031451607048282426 2216 60-61 -0.010267031714512598 2216 62-63 -0.003783497660791113 2216 64-65 0.04703351951916801 2216 66-67 -0.05806994686405176 2216 68-69 -0.1249795514805001 2216 70-71 -0.09199637580520204 2216 72-73 -0.10046075622052086 2216 74-75 -0.016488289392263766 2216 76-77 -0.08699562976579145 2216 78-79 -0.0547422268287292 2216 80-81 0.07157551018816122 2216 82-83 0.05333129926640012 2216 84-85 0.11345231416303037 2216 86-87 0.09173187303319708 2216 88-89 0.016049631341104487 2216 90-91 0.17222875146826055 2216 92-93 0.3382440062922676 2216 94-95 0.2815815479349375 2216 96-97 0.257154406627329 2216 98-99 0.31220645576785344 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 5.0 7 255.0 8 1754.0 9 3619.0 10 5201.0 11 8069.0 12 15614.0 13 30422.0 14 47971.0 15 54419.0 16 53884.0 17 54390.0 18 51654.0 19 50915.0 20 51451.0 21 53500.0 22 56776.0 23 61170.0 24 68539.0 25 75750.0 26 83051.0 27 93503.0 28 112729.0 29 140446.0 30 177621.0 31 228893.0 32 305549.0 33 412746.0 34 562710.0 35 815000.0 36 1258500.0 37 1561204.0 38 462949.0 39 412.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.524676213546414 18.31097895001946 12.423006929502053 24.741337906932074 2 15.832309844841108 21.004124458690836 37.50970272837202 25.653862968096036 3 18.29158827376239 27.023442016451305 28.339135461789276 26.34583424799703 4 12.962414112087759 17.72390908864964 35.631593563321694 33.6820832359409 5 14.251413405403014 38.0303134568492 32.57743398589015 15.14083915185763 6 31.493185067933826 36.679208082094384 17.51302424723134 14.314582602740455 7 27.79127184721128 32.48070480561429 21.370410984803044 18.357612362371388 8 27.323687615748536 33.41299998232929 19.563185790565306 19.700126611356865 9 27.322926194902763 16.268603989471707 18.59770415811924 37.810765657506295 10-11 25.264417755127344 26.512666666762442 25.86491302347144 22.35800255463877 12-13 26.37266579615672 24.526069397619857 25.49433093447456 23.606933871748858 14-15 24.639298423959413 25.108376764251606 23.070448236958764 27.181876574830216 16-17 24.084768551767493 27.330202792230807 24.339119030334864 24.245909625666837 18-19 24.71820604651477 26.745438765889094 25.515499870630286 23.02085531696585 20-21 26.172377827326223 26.052927604358224 24.317641768573004 23.457052799742552 22-23 25.21474581976364 26.161701939367628 23.82530362374547 24.798248617123264 24-25 24.538813299716296 26.752664625488425 23.86169305347612 24.84682902131916 26-27 24.892822801946192 26.332993279812616 23.92628495910379 24.847898959137403 28-29 24.42127495263153 26.271854599006833 24.77512234983522 24.531748098526414 30-31 24.913730887779366 26.497071422327128 24.392449162119846 24.196748527773664 32-33 23.977373431121862 26.953568311310416 24.1209085365604 24.948149721007322 34-35 24.181552990428003 27.11034537995099 24.629656566701396 24.078445062919613 36-37 24.65605686578205 26.84301556559705 24.136803765039318 24.36412380358158 38-39 24.784359838923553 26.510910587630214 24.095144576599665 24.609584996846568 40-41 24.307749928131926 26.957695601472903 24.527247444966154 24.207307025429014 42-43 24.888304590175288 26.705234308588928 24.342430492692444 24.064030608543344 44-45 24.434756361850745 26.959132244578143 24.241463215256115 24.364648178314994 46-47 23.805564147479462 26.946999793554387 25.077898380773924 24.16953767819223 48-49 24.472828101774596 26.942580775247276 24.729240697339396 23.85535042563873 50-51 23.5654436188695 27.273907932151943 24.495849609901114 24.66479883907744 52-53 23.564279937954257 27.057858358770297 24.04141784606685 25.336443857208597 54-55 23.927750643580197 26.789795121763404 24.134318372467252 25.148135862189147 56-57 24.279613272915785 26.134405720368047 24.042567160551044 25.54341384616512 58-59 23.9966664133386 26.200182424941502 24.88477763135192 24.918373530367976 60-61 24.17073009196958 26.57290511216519 24.421941505352 24.83442329051323 62-63 23.588279061027308 26.672500395436018 24.415433512085258 25.323787031451424 64-65 23.480724774953448 26.562431983927986 24.5494363977266 25.407406843391968 66-67 24.528432540033148 26.382349167874526 24.211896873705673 24.877321418386654 68-69 24.582680319181872 26.028647238175743 24.255506401609843 25.13316604103254 70-71 23.929991806824372 26.359786290718233 24.357429046711157 25.352792855746237 72-73 24.75331042079133 26.205160393301163 24.413551509617392 24.627977676290115 74-75 25.04617011779468 25.96060782071154 24.43276661114999 24.560455450343795 76-77 24.49538270089191 26.228958386339478 24.814295920608803 24.461362992159806 78-79 24.75190251054819 26.142357539955558 24.70802024689861 24.397719702597637 80-81 24.37461129824984 25.96129740940205 25.04297358688552 24.62111770546259 82-83 24.600250464358968 26.03740357790219 24.485872123535216 24.876473834203626 84-85 24.7503150199169 26.08416631097778 24.196115575639187 24.969403093466134 86-87 24.69129053793808 25.48959001222727 24.564693547504255 25.25442590233039 88-89 24.881552367580653 25.01030432267234 24.831988180449844 25.276155129297162 90-91 25.190409085560862 25.380290204780543 24.47500391844407 24.954296791214524 92-93 24.81668793137903 25.448336805460276 24.61146346379537 25.12351179936532 94-95 24.17149151281536 25.74497487383041 24.955323991034774 25.12820962231946 96-97 24.90648530867211 25.70013005930032 24.328954780365283 25.06442985166229 98-99 24.72518094879071 25.967870051608532 24.05584174284347 25.251107256757287 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 847.0 1 763.5 2 718.5 3 711.0 4 641.0 5 618.5 6 592.0 7 565.5 8 565.5 9 560.0 10 538.0 11 530.0 12 545.5 13 605.0 14 717.0 15 922.0 16 1123.5 17 1339.5 18 1787.0 19 2372.5 20 3139.5 21 4343.0 22 6257.0 23 8850.0 24 11972.0 25 15914.0 26 21287.0 27 27353.5 28 34506.5 29 43229.0 30 50828.0 31 59232.0 32 68468.0 33 77316.5 34 85363.5 35 93655.5 36 100910.5 37 109181.5 38 122158.0 39 125837.0 40 126564.5 41 135014.5 42 149188.0 43 170193.0 44 194028.0 45 221640.0 46 256104.5 47 290577.0 48 319409.0 49 353856.0 50 388771.5 51 360914.5 52 314855.0 53 308176.5 54 295046.5 55 280982.0 56 259070.5 57 237504.0 58 224705.5 59 198490.0 60 165228.0 61 130468.0 62 106498.5 63 86680.0 64 65056.5 65 49458.0 66 36319.0 67 27549.0 68 21623.5 69 18116.0 70 15795.0 71 13558.0 72 12962.5 73 11358.0 74 8092.5 75 6013.5 76 4446.5 77 3095.0 78 2078.5 79 1375.5 80 931.5 81 677.5 82 487.5 83 345.0 84 238.5 85 189.5 86 141.0 87 95.0 88 72.5 89 53.0 90 37.0 91 21.0 92 15.0 93 16.5 94 10.5 95 6.0 96 6.5 97 5.0 98 3.5 99 9.0 100 14.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0020400332094420207 2 0.0 3 1.0056501736686018E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 4.453593626246665E-4 22-23 0.0 24-25 0.0044823264883514825 26-27 2.8732862104817193E-5 28-29 0.00874197329539063 30-31 5.674740265701395E-4 32-33 6.464893973583869E-5 34-35 5.028250868343009E-5 36-37 0.0 38-39 1.4366431052408597E-5 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 2.0831325025992466E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.8732862104817193E-5 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 6960671.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.94599857116714 #Duplication Level Percentage of deduplicated Percentage of total 1 74.83131674861227 30.640429886672333 2 13.677527026474998 11.200800041662905 3 4.5853424218772805 5.632542727634977 4 2.1336827230017468 3.4946307890941415 5 1.1609790912776572 2.3768724106304937 6 0.7384807779247057 1.8142699726643639 7 0.4625148486935784 1.325669263362559 8 0.3479340894384922 1.1397206983207073 9 0.26267656988194554 0.9679999009558706 >10 1.4957966309232833 11.531620388810074 >50 0.1501269604363032 4.267501182011565 >100 0.12438410901803575 10.53388422573847 >500 0.01860926955278313 5.189995475630796 >1k 0.010169684622890588 7.432721827735684 >5k 2.471798345471822E-4 0.7972909856674673 >10k+ 2.1186842961187045E-4 1.6540502234077326 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 35918 0.516013470540412 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 31955 0.4590793042797167 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 14216 0.20423318384104064 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 11962 0.17185124824891163 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 10227 0.14692549037298272 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 10121 0.1454026486814274 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 9900 0.1422276674188451 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 9249 0.13287512080372713 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 9152 0.13148157699164348 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 8501 0.12212903037652548 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 1.4366431052408597E-5 0.0 1.4366431052408597E-5 0.0 5 1.4366431052408597E-5 1.4366431052408597E-5 0.0 4.309929315722579E-5 0.0 6 1.4366431052408597E-5 1.4366431052408597E-5 0.0 4.309929315722579E-5 0.0 7 1.4366431052408597E-5 1.4366431052408597E-5 0.0 7.183215526204298E-5 0.0 8 1.4366431052408597E-5 1.4366431052408597E-5 0.0 7.183215526204298E-5 0.0 9 1.4366431052408597E-5 2.8732862104817193E-5 0.0 1.0056501736686017E-4 1.4366431052408597E-5 10-11 2.8732862104817193E-5 2.8732862104817193E-5 0.0 1.6521395710269887E-4 1.4366431052408597E-5 12-13 4.309929315722579E-5 2.8732862104817193E-5 0.0 1.7958038815510746E-4 1.4366431052408597E-5 14-15 5.7465724209634386E-5 4.309929315722579E-5 0.0 2.2986289683853757E-4 2.8732862104817193E-5 16-17 5.7465724209634386E-5 4.309929315722579E-5 0.0 3.1606148315298916E-4 5.7465724209634386E-5 18-19 7.901537078824729E-5 4.309929315722579E-5 0.0 3.5197756078401065E-4 8.619858631445158E-5 20-21 1.0056501736686017E-4 4.309929315722579E-5 0.0 6.824054749894083E-4 8.619858631445158E-5 22-23 1.0056501736686017E-4 4.309929315722579E-5 0.0 0.002672156175747999 1.0056501736686017E-4 24-25 1.0774823289306447E-4 4.309929315722579E-5 0.0 0.007650124535407577 1.1493144841926877E-4 26-27 1.8676360368131175E-4 4.309929315722579E-5 0.0 0.01330331515453036 1.1493144841926877E-4 28-29 2.0831325025992466E-4 4.309929315722579E-5 0.0 0.03422083876683728 1.1493144841926877E-4 30-31 2.2986289683853757E-4 5.7465724209634386E-5 0.0 0.08935201793045527 1.1493144841926877E-4 32-33 2.514125434171505E-4 5.7465724209634386E-5 0.0 0.17474608410597198 1.1493144841926877E-4 34-35 2.65778974469559E-4 7.183215526204298E-5 0.0 0.2812372542819507 1.1493144841926877E-4 36-37 2.7296218999576334E-4 8.619858631445158E-5 0.0 0.44159535768893543 1.1493144841926877E-4 38-39 2.7296218999576334E-4 8.619858631445158E-5 0.0 0.6773269416123819 1.1493144841926877E-4 40-41 2.8014540552196766E-4 1.0056501736686017E-4 0.0 0.9718675110488629 1.1493144841926877E-4 42-43 3.1606148315298916E-4 1.0056501736686017E-4 0.0 1.2224324350339213 1.1493144841926877E-4 44-45 3.591607763102149E-4 1.0056501736686017E-4 0.0 1.5032315131687735 1.1493144841926877E-4 46-47 3.591607763102149E-4 1.0056501736686017E-4 0.0 1.8123396436923969 1.1493144841926877E-4 48-49 3.591607763102149E-4 1.0056501736686017E-4 0.0 2.1261168643080532 1.1493144841926877E-4 50-51 3.591607763102149E-4 1.0056501736686017E-4 0.0 2.480601367310709 1.1493144841926877E-4 52-53 3.591607763102149E-4 1.0056501736686017E-4 0.0 2.8408396259498545 1.2211466394547308E-4 54-55 3.591607763102149E-4 1.0056501736686017E-4 0.0 3.182832517152441 1.2929787947167737E-4 56-57 3.735272073626235E-4 1.0056501736686017E-4 0.0 3.5626737709625984 1.2929787947167737E-4 58-59 3.735272073626235E-4 1.0056501736686017E-4 0.0 3.972935942526231 1.2929787947167737E-4 60-61 3.735272073626235E-4 1.0056501736686017E-4 0.0 4.383808687409591 1.2929787947167737E-4 62-63 3.735272073626235E-4 1.2929787947167737E-4 0.0 4.7951052420089955 1.4366431052408596E-4 64-65 3.735272073626235E-4 1.4366431052408596E-4 0.0 5.234308588927705 1.4366431052408596E-4 66-67 3.735272073626235E-4 1.4366431052408596E-4 0.0 5.702862554486486 1.5084752605029026E-4 68-69 3.735272073626235E-4 1.5084752605029026E-4 0.0 6.214630744650911 1.5803074157649458E-4 70-71 3.735272073626235E-4 1.5803074157649458E-4 0.0 6.801693399961009 1.5803074157649458E-4 72-73 3.735272073626235E-4 1.5803074157649458E-4 0.0 7.346166770416243 1.5803074157649458E-4 74-75 3.735272073626235E-4 1.7239717262890317E-4 0.0 7.871963780503345 1.5803074157649458E-4 76-77 3.735272073626235E-4 1.7958038815510746E-4 0.0 8.434409556205141 1.5803074157649458E-4 78-79 3.8071042288882783E-4 1.8676360368131175E-4 0.0 9.007852834877557 1.5803074157649458E-4 80-81 3.878936384150321E-4 1.8676360368131175E-4 0.0 9.623361023671425 1.5803074157649458E-4 82-83 3.878936384150321E-4 1.8676360368131175E-4 0.0 10.290308218848441 1.5803074157649458E-4 84-85 3.878936384150321E-4 1.8676360368131175E-4 0.0 10.926618999806198 1.5803074157649458E-4 86-87 3.878936384150321E-4 1.8676360368131175E-4 0.0 11.575019132494553 1.5803074157649458E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACAAG 3810 0.0 26.484129 1 GTACATA 2220 0.0 26.18231 1 GTACTAG 2515 0.0 25.699686 1 GTACAAA 4460 0.0 21.060444 1 GTATAAG 2875 0.0 20.379036 1 TCTAACG 1290 0.0 20.185665 2 CTAACGC 1320 0.0 20.079165 3 TAGGCAT 3685 0.0 19.811024 5 CTAGGCA 4150 0.0 19.383968 4 TAGTACT 3055 0.0 19.178034 4 GTGATCG 2135 0.0 18.730326 8 GTATAAT 2055 0.0 18.102108 1 TGATCGC 2200 0.0 17.965569 9 TACATGA 2845 0.0 17.815128 2 CTAGTAC 3485 0.0 17.34544 3 TACATAG 2645 0.0 16.876808 2 GTATATA 1655 0.0 16.85793 1 ACGCCTA 1550 0.0 16.799683 6 CAACACA 9710 0.0 16.760756 5 TAAGGTG 2840 0.0 16.53666 4 >>END_MODULE