FastQCFastQC Report
Fri 27 May 2016
ERR765547_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765547_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6960671
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT353380.507680940530015No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA347310.49896051688120296No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229140.3291924011348906No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155390.22323997212337718No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG137000.1968201054179978No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117330.16856133553791006No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG113700.16334632106588576No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC104730.15045963241187524No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT103780.14909482146189643No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC103650.1489080578582151No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG102560.14734211687350257No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA93270.133995702425815No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC85600.12297664980861758No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC74880.10757583572043557No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG6850.034.6209562
TAACGCA6500.025.7542294
TCACGCA10100.025.3221634
AGTACTT398700.022.28391312-13
GTATCAA721600.021.678021
CTAACGC11150.021.2693753
TCTAACG12300.020.0368842
TACTTTT460950.019.3301614-15
ATCACGC12150.019.1360873
GAGTACT385150.018.82981712-13
TAGGCAT36300.018.4465565
TAGTACT29650.018.3493464
CAACGCA846800.018.3081385
ATCAACG852950.018.1815833
AACGCAG872400.017.967476
TATCAAC870550.017.9208342
TCAACGC875400.017.7259314
CTAGGCA41050.017.2182834
GTATAAG27800.017.0715831
ACGCAGA923550.016.8464877
Produced by FastQC (version 0.11.5)