FastQCFastQC Report
Fri 27 May 2016
ERR765546_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765546_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7506646
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA239600.31918382723789024No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT218230.2907157204429248No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG91020.1212525540700867No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG84360.11238041596739742No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG25450.023.7531491
TCTAACG12900.021.9879882
CTAACGC13200.020.7837283
GTACAAG44600.020.4355721
TAGTACT35200.018.7581964
GTACAAA51200.018.6187061
GTATAAT24150.018.4850121
GTATAAG36100.018.0337661
GTGATCG22100.017.8843028
GTACATA23500.017.8090291
ACGCCTA16200.017.7959846
GTATCAA472650.017.7386951
TAGGCAT40900.017.7356325
CGGGCGT20450.017.2808726
GTCCTAC25650.017.2227251
TGATCGC23550.017.1780439
CATGGGG204300.017.1157074
GTCTAAC36550.017.0483761
TACATGA26400.016.9087962
CTAGGCA45950.016.8995974
Produced by FastQC (version 0.11.5)