##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765546_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7506646 Sequences flagged as poor quality 0 Sequence length 99 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.308925584075766 33.0 33.0 33.0 27.0 33.0 2 31.174637780974354 33.0 33.0 33.0 27.0 33.0 3 31.188267303400213 33.0 33.0 33.0 27.0 33.0 4 34.7470091169878 37.0 37.0 37.0 27.0 37.0 5 34.93808100182159 37.0 37.0 37.0 33.0 37.0 6 34.82355875047258 37.0 37.0 37.0 33.0 37.0 7 34.7262938734556 37.0 37.0 37.0 33.0 37.0 8 34.648813597977046 37.0 37.0 37.0 27.0 37.0 9 34.51711776471143 37.0 37.0 37.0 27.0 37.0 10-11 34.543313018890196 37.0 37.0 37.0 27.0 37.0 12-13 34.49180372965503 37.0 37.0 37.0 27.0 37.0 14-15 35.94697458758546 37.0 37.0 40.0 27.0 40.0 16-17 36.02739006208631 37.0 37.0 40.0 27.0 40.0 18-19 36.057247870753464 37.0 37.0 40.0 27.0 40.0 20-21 36.0888315500691 37.0 37.0 40.0 27.0 40.0 22-23 36.16704730714623 37.0 37.0 40.0 27.0 40.0 24-25 36.15997830455839 37.0 37.0 40.0 27.0 40.0 26-27 36.191055232922935 37.0 37.0 40.0 27.0 40.0 28-29 36.13621829243047 37.0 37.0 40.0 27.0 40.0 30-31 36.10037871773892 37.0 37.0 40.0 27.0 40.0 32-33 35.96836569887537 37.0 37.0 40.0 27.0 40.0 34-35 35.91222731163825 37.0 37.0 40.0 27.0 40.0 36-37 35.80804529746041 37.0 37.0 40.0 27.0 40.0 38-39 35.59960387102309 37.0 37.0 40.0 27.0 40.0 40-41 35.50945841858001 37.0 35.0 40.0 27.0 40.0 42-43 35.375021414357356 37.0 33.0 40.0 27.0 40.0 44-45 35.23468483794227 37.0 33.0 40.0 27.0 40.0 46-47 35.123931979741684 37.0 33.0 40.0 27.0 40.0 48-49 34.96470314438699 37.0 33.0 40.0 27.0 40.0 50-51 34.80424060226098 37.0 33.0 40.0 27.0 40.0 52-53 34.63133608538354 37.0 33.0 40.0 27.0 40.0 54-55 34.51047351906564 37.0 33.0 40.0 27.0 40.0 56-57 34.32181209823935 37.0 33.0 40.0 27.0 40.0 58-59 34.085452078065224 37.0 33.0 38.5 27.0 40.0 60-61 33.85028946349675 37.0 33.0 37.0 27.0 40.0 62-63 33.65762978565927 37.0 33.0 37.0 22.0 40.0 64-65 33.340228911820276 37.0 33.0 37.0 22.0 40.0 66-67 33.11509767478046 37.0 33.0 37.0 22.0 40.0 68-69 32.80941688205358 37.0 33.0 37.0 22.0 40.0 70-71 32.65505773150885 37.0 33.0 37.0 22.0 37.0 72-73 32.37712994591726 35.0 33.0 37.0 22.0 37.0 74-75 32.09495525698161 33.0 33.0 37.0 22.0 37.0 76-77 31.89188640306203 33.0 33.0 37.0 22.0 37.0 78-79 31.651904991923157 33.0 33.0 37.0 22.0 37.0 80-81 31.399770483382326 33.0 30.0 37.0 22.0 37.0 82-83 31.21204656513708 33.0 27.0 37.0 22.0 37.0 84-85 31.031182834517573 33.0 27.0 37.0 22.0 37.0 86-87 30.820223572551576 33.0 27.0 37.0 15.0 37.0 88-89 30.644616250719697 33.0 27.0 37.0 15.0 37.0 90-91 30.441013336715226 33.0 27.0 37.0 15.0 37.0 92-93 30.277036641930366 33.0 27.0 37.0 15.0 37.0 94-95 30.039194734905575 33.0 27.0 37.0 15.0 37.0 96-97 29.946079980326765 33.0 27.0 37.0 15.0 37.0 98-99 28.793791861238695 33.0 24.5 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3059585357429988 1101 2 0.2727970909491084 1101 3 0.3007875986974824 1101 4 0.3912162444162348 1101 5 0.37791827022146407 1101 6 0.2230915320719049 1101 7 0.17408728192027922 1101 8 0.2572781598808689 1101 9 0.2448446932053443 1101 10-11 0.2500327185941984 1101 12-13 0.2588325072578286 1101 14-15 0.3858571895719862 1101 16-17 0.35473942772745204 1101 18-19 0.2792633277714174 1101 20-21 0.3238867247276076 1101 22-23 0.25468925122426356 1101 24-25 0.37816439598315554 1101 26-27 0.38787606786566187 1101 28-29 0.3035009495081553 1101 30-31 0.3509457728487462 1101 32-33 0.4543185761004196 1101 34-35 0.41327440359717116 1101 36-37 0.36160534954051116 1101 38-39 0.3846913413448334 1101 40-41 0.2817044342509547 1101 42-43 0.3386027054500076 1101 44-45 0.389019518964254 1101 46-47 0.31843981659316967 1101 48-49 0.36035914203845465 1101 50-51 0.34103727282572294 1101 52-53 0.2222049265706545 1101 54-55 0.3831757056031151 1101 56-57 0.3387347692345628 1101 58-59 0.28104544766883066 1101 60-61 0.3280330928276243 1101 62-63 0.3458602027480282 1101 64-65 0.21122287271792572 1101 66-67 0.33376143725128316 1101 68-69 0.3067045706875504 1101 70-71 0.23171122857836224 1101 72-73 0.3314594367363455 1101 74-75 0.3240816111675642 1101 76-77 0.22106710149936504 1101 78-79 0.2685942989085319 1101 80-81 0.24237201607295944 1101 82-83 0.3388816092826801 1101 84-85 0.1810010799522601 1101 86-87 0.19167265500706776 1101 88-89 0.24948003762821358 1101 90-91 0.21204588597496965 1101 92-93 0.25231179800142556 1101 94-95 0.23360700385750022 1101 96-97 0.17039882428597863 1101 98-99 0.21110375652878588 1102 1 0.2827164828416464 1102 2 0.31357482624230215 1102 3 0.2770274326890956 1102 4 0.35291204941933785 1102 5 0.30710873327166865 1102 6 0.23987163151779356 1102 7 0.16047009154542735 1102 8 0.27006839214017475 1102 9 0.2803444528114767 1102 10-11 0.24170966598029509 1102 12-13 0.30378255023153145 1102 14-15 0.3411261934305898 1102 16-17 0.31370429762635155 1102 18-19 0.2965237356117285 1102 20-21 0.34538972090287245 1102 22-23 0.27740524979196834 1102 24-25 0.3512566521048157 1102 26-27 0.3589242091153153 1102 28-29 0.17613218307145928 1102 30-31 0.27098000161263514 1102 32-33 0.36897335084680094 1102 34-35 0.30856552784636904 1102 36-37 0.38545658400179406 1102 38-39 0.3474293244873863 1102 40-41 0.3820699623608945 1102 42-43 0.32617396677404287 1102 44-45 0.25567688092073126 1102 46-47 0.29251000201681165 1102 48-49 0.2744587898921935 1102 50-51 0.29809322843410513 1102 52-53 0.2752109684539903 1102 54-55 0.2939311463419969 1102 56-57 0.3486408023138381 1102 58-59 0.3110008090273908 1102 60-61 0.3456848098023073 1102 62-63 0.3428168275126069 1102 64-65 0.2766338136190569 1102 66-67 0.21830932820051174 1102 68-69 0.20598552217752086 1102 70-71 0.2840548331506483 1102 72-73 0.26512180901561777 1102 74-75 0.2980831936606947 1102 76-77 0.23611846621593457 1102 78-79 0.28628158363058986 1102 80-81 0.34013908535608905 1102 82-83 0.26332993770240876 1102 84-85 0.18424598977779638 1102 86-87 0.30237488792979406 1102 88-89 0.33346180060928177 1102 90-91 0.18991327995360052 1102 92-93 0.294377122499597 1102 94-95 0.3080230720427579 1102 96-97 0.2410452944586865 1102 98-99 0.3281424331784919 1103 1 0.23113889494701212 1103 2 0.2456938652904448 1103 3 0.3095000554131815 1103 4 0.3486283109528472 1103 5 0.27389555049756353 1103 6 0.2568438847907544 1103 7 0.14154187874157742 1103 8 0.1454145552165329 1103 9 0.2498416835068653 1103 10-11 0.25638646729137093 1103 12-13 0.18655089174330897 1103 14-15 0.21797672518759725 1103 16-17 0.1792639924028805 1103 18-19 0.21370250259226964 1103 20-21 0.19003323214113266 1103 22-23 0.1861371245827712 1103 24-25 0.20627956912672119 1103 26-27 0.26204512383841205 1103 28-29 0.27518696446374946 1103 30-31 0.33802563198467794 1103 32-33 0.3429613468428414 1103 34-35 0.288409482110211 1103 36-37 0.2831593163612922 1103 38-39 0.26480402687396776 1103 40-41 0.2498413115837792 1103 42-43 0.22033711260304045 1103 44-45 0.14317281537946513 1103 46-47 0.18029001123730382 1103 48-49 0.22127862878623716 1103 50-51 0.26000356706605743 1103 52-53 0.19317604269156874 1103 54-55 0.28033988401906385 1103 56-57 0.20053905484158463 1103 58-59 0.20056527509157718 1103 60-61 0.2175221344709044 1103 62-63 0.21081742589712604 1103 64-65 0.2129892650757057 1103 66-67 0.2229134944724649 1103 68-69 0.24285046884394035 1103 70-71 0.21512129172475625 1103 72-73 0.24382942476118075 1103 74-75 0.24428810957108738 1103 76-77 0.2384330617458872 1103 78-79 0.20361855147886843 1103 80-81 0.1536782061255586 1103 82-83 0.19364281070595268 1103 84-85 0.20613300878084573 1103 86-87 0.2888979738887585 1103 88-89 0.3013453666652488 1103 90-91 0.22394158401503717 1103 92-93 0.27891993462264963 1103 94-95 0.17375896658814582 1103 96-97 0.04826443265866942 1103 98-99 0.1546723792449427 1104 1 0.2047288364590294 1104 2 0.15302294051616627 1104 3 0.14366069836905382 1104 4 0.2465006513783763 1104 5 0.16734131903877625 1104 6 0.1885599816923289 1104 7 0.08038055419696377 1104 8 0.10528904434185193 1104 9 0.20914934651620598 1104 10-11 0.13843628838620958 1104 12-13 0.07794044711677373 1104 14-15 0.21414096256552995 1104 16-17 0.17976984864754542 1104 18-19 0.13976512034931687 1104 20-21 0.11749217204408069 1104 22-23 0.18751636344048705 1104 24-25 0.13609771786072855 1104 26-27 0.1255385370484987 1104 28-29 -0.006013611638749694 1104 30-31 0.012021528351496613 1104 32-33 0.10430388574346239 1104 34-35 0.08207646992526918 1104 36-37 0.115081486120296 1104 38-39 0.08722562674113732 1104 40-41 0.12612684387258355 1104 42-43 0.04029021891756912 1104 44-45 0.009867370623013016 1104 46-47 0.01489909743942519 1104 48-49 0.039561133692977535 1104 50-51 0.08479844482054233 1104 52-53 0.02298829763174126 1104 54-55 0.1447435279363205 1104 56-57 0.05754156318953818 1104 58-59 0.11471593741457298 1104 60-61 0.12712047083669376 1104 62-63 0.08528519809375723 1104 64-65 0.07415952141137439 1104 66-67 0.17898493390802628 1104 68-69 0.08897341005619808 1104 70-71 0.05196787674653791 1104 72-73 0.1305930728547935 1104 74-75 0.0445715028246596 1104 76-77 -0.051837457053729 1104 78-79 0.03381195822478844 1104 80-81 0.06757071790205416 1104 82-83 0.15984367357638263 1104 84-85 0.08490538143212234 1104 86-87 0.18385958817987458 1104 88-89 0.25080995827286756 1104 90-91 0.22232283848687473 1104 92-93 0.2534619694137774 1104 94-95 0.15582570857496236 1104 96-97 0.03578777810279732 1104 98-99 0.11715761703417726 1105 1 0.12238213088526351 1105 2 0.1797622899189406 1105 3 0.15967701326541928 1105 4 0.032109282719190446 1105 5 0.10659440882206184 1105 6 0.06753293048183906 1105 7 0.017241703426719823 1105 8 0.012132154828506714 1105 9 -0.06436752061553364 1105 10-11 -0.0016109886068562673 1105 12-13 -0.030006064294688883 1105 14-15 0.03290094594361648 1105 16-17 0.04106184206530372 1105 18-19 0.01566968057849749 1105 20-21 0.06366993699941048 1105 22-23 0.042490656612443445 1105 24-25 0.05412567943253066 1105 26-27 0.12119625375838439 1105 28-29 0.0800726279851176 1105 30-31 0.08698756950075648 1105 32-33 0.11955152615642817 1105 34-35 0.04064117124198674 1105 36-37 0.06759846850015094 1105 38-39 -0.028879755668960172 1105 40-41 -0.07340520433432118 1105 42-43 -6.125033519097656E-4 1105 44-45 -0.04060008940438564 1105 46-47 9.737391679038865E-4 1105 48-49 -0.05586817981696868 1105 50-51 0.061941113232002465 1105 52-53 0.0052586632876341355 1105 54-55 0.1092076059996927 1105 56-57 0.10653331406690114 1105 58-59 0.010627586378184617 1105 60-61 0.03685418395153306 1105 62-63 0.051225937738749394 1105 64-65 -0.0865080353162 1105 66-67 0.013782057390500313 1105 68-69 -0.10272321291609643 1105 70-71 -0.12215677623890997 1105 72-73 0.01724164052249222 1105 74-75 -0.012992501568717785 1105 76-77 -0.1079273500496356 1105 78-79 -0.08789392790266248 1105 80-81 -0.07289111173860263 1105 82-83 -0.007877089936620507 1105 84-85 -0.053840835150055 1105 86-87 -0.02596958656895154 1105 88-89 -0.03916048703663577 1105 90-91 -0.04719064772268666 1105 92-93 0.025678067695324103 1105 94-95 -0.09351832847212194 1105 96-97 -0.1885490949094546 1105 98-99 0.0077444504038908235 1106 1 0.09201711477880536 1106 2 0.10449214322344957 1106 3 0.0367060513774895 1106 4 0.02959047587911101 1106 5 -0.013910138467188915 1106 6 -0.002105294735009977 1106 7 0.013673253948738306 1106 8 -0.012691371892074699 1106 9 -0.005994281732959905 1106 10-11 -0.0789321505116618 1106 12-13 0.02369741612454135 1106 14-15 0.020119947039681563 1106 16-17 -3.6676429859028303E-4 1106 18-19 0.058750203406575 1106 20-21 0.03405153101902414 1106 22-23 0.0055387322070501455 1106 24-25 0.07364174046031025 1106 26-27 0.10965079404105893 1106 28-29 5.523158516282933E-4 1106 30-31 -0.003039056965974396 1106 32-33 0.014916133693674283 1106 34-35 -0.11116886485630317 1106 36-37 -0.021158340037111145 1106 38-39 -0.034885884876686646 1106 40-41 0.0405644658309825 1106 42-43 -0.03280586200006752 1106 44-45 0.018671685820997652 1106 46-47 -0.07806764845366132 1106 48-49 -0.1055462887310128 1106 50-51 -0.08171314337884894 1106 52-53 -0.06491055898831632 1106 54-55 -0.09343685044870398 1106 56-57 -0.043683151643364226 1106 58-59 -0.0021196565769656672 1106 60-61 0.04178497838919526 1106 62-63 0.0733273396159575 1106 64-65 -0.059523947340139216 1106 66-67 -0.04296396046629525 1106 68-69 0.009826507151750263 1106 70-71 -0.03329228716529542 1106 72-73 0.09621454211937674 1106 74-75 -0.031086825972494125 1106 76-77 -0.07425395383462785 1106 78-79 0.024081455852829947 1106 80-81 -8.643184244405688E-5 1106 82-83 -0.0560153671808834 1106 84-85 -0.11501408014969527 1106 86-87 -0.12021282186364957 1106 88-89 0.019444981490256197 1106 90-91 -0.007276425368466732 1106 92-93 -0.03934179460583209 1106 94-95 -0.05694398927123956 1106 96-97 -0.14959092376922456 1106 98-99 0.21298098384705355 1107 1 0.03386996994790792 1107 2 0.09100613527257906 1107 3 0.048516106003027915 1107 4 -0.037346619485212784 1107 5 0.02670978784237832 1107 6 -0.04482089932640321 1107 7 -0.09695091244173426 1107 8 0.013832894065750168 1107 9 -0.11465679500541626 1107 10-11 -0.016538544385582554 1107 12-13 -0.09464483918569044 1107 14-15 -0.11283571457233421 1107 16-17 -0.13131343308723586 1107 18-19 -0.08801989390082099 1107 20-21 -0.10934916894398583 1107 22-23 -0.10669763527781839 1107 24-25 -0.015363991251469145 1107 26-27 0.07245674336409991 1107 28-29 -0.004288600104210616 1107 30-31 -0.04356764175528838 1107 32-33 -0.13415665387444164 1107 34-35 -0.11992821682968469 1107 36-37 -0.1423107114210822 1107 38-39 -0.07358181641430406 1107 40-41 -0.16110111344895017 1107 42-43 -0.1580979117232033 1107 44-45 -0.11031504679549187 1107 46-47 -0.19543121877177327 1107 48-49 -0.16333577342932415 1107 50-51 -0.11039264902457546 1107 52-53 -0.1523688387729365 1107 54-55 -0.15016657707865022 1107 56-57 -0.0854718454684118 1107 58-59 -0.037129397641663786 1107 60-61 0.005777123151382568 1107 62-63 -0.2139059885054877 1107 64-65 -0.2418069690074276 1107 66-67 -0.0700677233477549 1107 68-69 -0.12995270719731167 1107 70-71 -0.05335450426200339 1107 72-73 -0.08900384405355766 1107 74-75 -0.13562941870653233 1107 76-77 -0.23552017711768514 1107 78-79 0.01705820569556593 1107 80-81 -0.22844116296137784 1107 82-83 -0.14504670538643438 1107 84-85 -0.25440282039209805 1107 86-87 -0.23200000677020682 1107 88-89 -0.16609384955199857 1107 90-91 -0.16825220787282547 1107 92-93 -0.13775976449656469 1107 94-95 -0.1669211070720955 1107 96-97 -0.19423102529308167 1107 98-99 0.12407216172945112 1108 1 0.03692336748965985 1108 2 0.1025460634371207 1108 3 0.04045970381118735 1108 4 0.09499324207843074 1108 5 0.10531331977098546 1108 6 -0.0943459248397076 1108 7 0.024055097338575138 1108 8 -0.09750235154116638 1108 9 -0.056082857239758255 1108 10-11 -0.06125607883328854 1108 12-13 -0.020804455672944755 1108 14-15 -0.04682051566810941 1108 16-17 -0.02129512525803534 1108 18-19 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2215 24-25 -0.06665481823822716 2215 26-27 0.015246985267594937 2215 28-29 0.055740790174944266 2215 30-31 -0.09165794087202528 2215 32-33 -0.04549090623336838 2215 34-35 -0.04937179198280006 2215 36-37 -0.056037436999623935 2215 38-39 -0.016945340583880864 2215 40-41 -0.03385772811114407 2215 42-43 0.042184591980678476 2215 44-45 0.0954615184483103 2215 46-47 0.07075389600629478 2215 48-49 0.10396005108363227 2215 50-51 0.04767476566834006 2215 52-53 0.07859986202555547 2215 54-55 0.11421272233182833 2215 56-57 0.13951939802404212 2215 58-59 -0.03474376562796522 2215 60-61 0.024412301694951566 2215 62-63 0.02654964417021688 2215 64-65 -0.03597911663540998 2215 66-67 -0.1267272414656162 2215 68-69 0.11460112476172668 2215 70-71 -0.08559084309028009 2215 72-73 -0.12756536496159754 2215 74-75 0.04736892155420236 2215 76-77 0.025596025542476752 2215 78-79 0.08204798950597336 2215 80-81 0.1963386316350757 2215 82-83 0.07765656776273744 2215 84-85 0.14729496798460673 2215 86-87 0.11953735515923825 2215 88-89 -0.018101063075963708 2215 90-91 0.20835258005825708 2215 92-93 0.19293380855765463 2215 94-95 0.16181209802869745 2215 96-97 0.18116625439124334 2215 98-99 0.12656344300194533 2216 1 -0.06330075916218192 2216 2 -0.09969553534209652 2216 3 0.018033336088578267 2216 4 -0.08065797556854903 2216 5 -0.1699649467177835 2216 6 -0.056804240597344346 2216 7 0.001174098965442738 2216 8 -0.07320422221841483 2216 9 -0.018887651469484013 2216 10-11 -0.06667318552014478 2216 12-13 -0.05719087039938131 2216 14-15 -0.11962308761396656 2216 16-17 -0.07054390934447952 2216 18-19 -0.11497501364750207 2216 20-21 -0.20580444466781245 2216 22-23 -0.24662905674070856 2216 24-25 -0.19264116432007938 2216 26-27 -0.24504672619263346 2216 28-29 -0.09452331900969568 2216 30-31 -0.1438448549564768 2216 32-33 -0.13433248418844812 2216 34-35 -0.23256550349196914 2216 36-37 -0.2105084927467047 2216 38-39 -0.12389482601947321 2216 40-41 -0.16061143006092493 2216 42-43 -0.17762925602612967 2216 44-45 -0.14458553428212184 2216 46-47 -0.06901219063671249 2216 48-49 0.03523764112277661 2216 50-51 0.015117037685399737 2216 52-53 0.06830561634458832 2216 54-55 -0.04715102551545414 2216 56-57 0.11740222356255003 2216 58-59 -0.02658696399691962 2216 60-61 0.00928490723848796 2216 62-63 -0.006996702571086644 2216 64-65 0.11593160114703949 2216 66-67 0.02284017821274631 2216 68-69 -0.08310286372794451 2216 70-71 -0.04805980949109312 2216 72-73 -0.10313083940793177 2216 74-75 0.04432111462346455 2216 76-77 -0.053624705407351314 2216 78-79 0.04015236945828349 2216 80-81 0.14743483528818757 2216 82-83 0.04176287000244017 2216 84-85 0.08782773242318953 2216 86-87 0.01708061542975514 2216 88-89 0.0019909760548770805 2216 90-91 0.1241037873583366 2216 92-93 0.23271716455766978 2216 94-95 0.173167147926641 2216 96-97 0.1903527650106973 2216 98-99 0.25452312589376547 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 9.0 7 284.0 8 1874.0 9 3734.0 10 5514.0 11 9018.0 12 17155.0 13 30014.0 14 42234.0 15 46625.0 16 47820.0 17 49690.0 18 51175.0 19 52701.0 20 55830.0 21 60175.0 22 65419.0 23 71439.0 24 79745.0 25 88178.0 26 95067.0 27 105098.0 28 123502.0 29 151635.0 30 189241.0 31 242654.0 32 319920.0 33 430581.0 34 588367.0 35 849322.0 36 1333180.0 37 1752671.0 38 546236.0 39 539.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.56024240438076 18.31998150927234 12.414458736569078 24.705317349777825 2 15.72158857630958 20.78423572924579 38.22604662588326 25.26812906856138 3 18.523288124895345 26.700998063447052 28.487337272336465 26.288376539321135 4 13.039591849675608 17.44784554913073 35.87383766331861 33.638724937875054 5 14.139963440396683 38.07191653902422 33.04535740728949 14.742762613289612 6 31.64012263266444 36.69127330634747 17.527508290653376 14.141095770334713 7 27.64514271753324 32.254871216785766 21.57613400178988 18.523852063891116 8 27.027103715827284 33.873796633010265 19.598046850750656 19.501052800411795 9 27.105594162825845 16.116185577420328 18.746135091490927 38.032085168262896 10-11 25.281457258008437 26.415965265978976 26.35138249492516 21.951194981087426 12-13 26.291468919674642 24.333657135290515 25.884343020837804 23.49053092419704 14-15 24.391965466334764 24.82999065095117 23.53197419992897 27.246069682785095 16-17 23.983713898324233 27.235505710539698 24.383026720588663 24.397753670547406 18-19 24.420846540518895 26.81549123270233 25.58388260216347 23.179779624615307 20-21 26.12364819319481 26.040488262360423 24.555991911139767 23.279871633305003 22-23 25.443740120421293 25.960349002737043 24.188898743859774 24.407012132981894 24-25 24.45915948631167 26.440767608286503 24.397144932541686 24.702927972860138 26-27 24.729965880891648 26.20231490929902 24.233991914362797 24.83372729544654 28-29 24.553541482244476 26.18722370810986 24.726483393892757 24.5327514157529 30-31 24.77387580670623 26.330211860088546 24.552830473962597 24.343081859242627 32-33 24.41895798161708 26.698811972275756 24.262150204825247 24.620079841281914 34-35 24.52369527581167 26.723133533440507 24.642103715055622 24.1110674756922 36-37 24.602179188948035 26.391347080973315 24.509274847914767 24.497198882163886 38-39 24.710168438108397 26.35262000218606 24.468895523159514 24.46831603654602 40-41 24.473906189262156 26.48378516850268 24.745672035153916 24.296636607081247 42-43 24.811233938565906 26.3596018781224 24.560422857292057 24.268741326019637 44-45 24.421532599246053 26.62249558591147 24.487194414123163 24.46877740071931 46-47 23.95354729662222 26.612417849462993 25.064056570670846 24.36997828324394 48-49 24.416730726691462 26.49529286205137 24.89386218039343 24.194114230863736 50-51 23.732669690298437 26.721814243005465 24.82361629947649 24.721899767219607 52-53 23.90592283158151 26.60965363226133 24.374813998155766 25.109609538001394 54-55 24.07578564381483 26.490652416538623 24.401317179470034 25.032244760176514 56-57 24.340983976066006 26.02349970945746 24.455082869233475 25.180433445243054 58-59 24.073314500244184 26.02533141965133 25.0364610240046 24.864893056099888 60-61 24.303437247473774 26.20836922375186 24.67582059950609 24.812372929268278 62-63 23.99629608216506 26.248959921648098 24.573950869669357 25.180793126517486 64-65 23.75926612231348 26.1535378116938 24.655418678328513 25.431777387664212 66-67 24.567874270787314 26.12361681633748 24.390085018724424 24.918423894150784 68-69 24.44593097902845 25.86697840819988 24.581097869807632 25.105992742964034 70-71 24.149786735647318 25.97951202174713 24.472087800596963 25.39861344200859 72-73 24.717410412053532 26.10639292168566 24.470962131423274 24.70523453483753 74-75 24.81899372902359 26.06626181652898 24.5246279097216 24.590116544725834 76-77 24.477622895764632 26.12681482515627 24.76177110256698 24.63379117651212 78-79 24.616419903109858 26.25729919860348 24.6723436805199 24.453937217766764 80-81 24.427573912503668 26.06400381741726 24.992047047376417 24.516375222702656 82-83 24.64506785054204 25.948765933547417 24.698853522598508 24.707312693312034 84-85 24.67028550433842 26.11235430577118 24.355917409719336 24.861442780171064 86-87 24.56165509869521 25.6314138165034 24.812099837930283 24.994831246871104 88-89 24.758700490205612 25.142933342001207 24.946161041828802 25.15220512596438 90-91 24.937961640924588 25.48957283985418 24.719342033712525 24.853123485508707 92-93 24.846642561804565 25.439903520160666 24.77187548207282 24.941578435961947 94-95 24.342436022692425 25.601953255821574 25.09617477632487 24.95943594516113 96-97 24.791817810510846 25.672797145356263 24.60554287494042 24.929842169192472 98-99 24.750148068791308 25.86433408475636 24.252995279116664 25.132522567335663 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1087.0 1 1036.0 2 1046.5 3 1068.0 4 986.5 5 882.0 6 814.5 7 793.0 8 814.5 9 840.0 10 798.0 11 760.0 12 776.5 13 820.0 14 888.5 15 1080.0 16 1249.0 17 1467.5 18 1931.0 19 2561.0 20 3476.5 21 4770.5 22 6353.5 23 8576.0 24 11373.5 25 15250.0 26 20981.5 27 26867.0 28 33782.0 29 42336.5 30 49736.5 31 58202.5 32 67853.5 33 78641.5 34 88375.0 35 96868.0 36 106527.5 37 117460.0 38 131049.5 39 136257.5 40 142246.0 41 155931.0 42 169696.0 43 190648.0 44 217141.5 45 252177.5 46 289503.5 47 321236.0 48 344911.5 49 368355.5 50 398388.0 51 385085.5 52 351250.5 53 345147.0 54 329970.0 55 311647.5 56 290723.0 57 266135.0 58 246499.0 59 213373.0 60 173933.5 61 136222.5 62 108199.0 63 85565.0 64 63085.0 65 46751.0 66 34998.5 67 26701.5 68 20810.0 69 17308.5 70 14866.5 71 12640.5 72 11546.0 73 9849.0 74 7245.0 75 5442.0 76 4214.5 77 3142.0 78 2225.5 79 1598.0 80 1222.0 81 891.5 82 634.0 83 459.0 84 334.5 85 255.5 86 158.0 87 103.0 88 67.0 89 45.5 90 40.0 91 28.0 92 19.5 93 14.5 94 10.5 95 9.5 96 7.0 97 7.0 98 9.0 99 16.5 100 23.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0024111966915717088 2 0.0 3 1.1989375814444959E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 3.4635974575063217E-4 22-23 0.0 24-25 0.00457594510251316 26-27 2.664305736543324E-5 28-29 0.008758905108886179 30-31 5.461826759913815E-4 32-33 3.996458604814987E-5 34-35 2.664305736543324E-5 36-37 0.0 38-39 1.9982293024074934E-5 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 1.93162165899391E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 7.326840775494141E-5 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 7506646.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.57635228063681 #Duplication Level Percentage of deduplicated Percentage of total 1 73.67080200504248 26.946092069334775 2 13.207876388258205 9.66191879312074 3 4.852169580126209 5.324239916642575 4 2.2969874936760446 3.3606169501164804 5 1.3221762430223793 2.4180192020937707 6 0.8402166812842331 1.8439236796031786 7 0.5792877503219648 1.4831762979343632 8 0.415590454002001 1.216062628003758 9 0.31542122421613705 1.0383262032347245 >10 2.0377507776882546 14.373161232704707 >50 0.23382561490252304 5.966622711004529 >100 0.19703260187351593 14.476049686029825 >500 0.02144306082131356 5.275660483290558 >1k 0.009016928763483532 5.202038442968391 >5k 3.298876374858874E-4 0.800289886627827 >10k+ 7.330836388575276E-5 0.6138018172898296 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 23960 0.31918382723789024 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 21823 0.2907157204429248 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 9102 0.1212525540700867 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 8436 0.11238041596739742 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 1.3321528682716622E-5 3 0.0 0.0 0.0 0.0 1.3321528682716622E-5 4 0.0 3.996458604814987E-5 0.0 1.3321528682716622E-5 1.3321528682716622E-5 5 2.6643057365433244E-5 3.996458604814987E-5 0.0 5.328611473086649E-5 1.3321528682716622E-5 6 2.6643057365433244E-5 3.996458604814987E-5 0.0 5.328611473086649E-5 1.3321528682716622E-5 7 2.6643057365433244E-5 3.996458604814987E-5 0.0 5.328611473086649E-5 1.3321528682716622E-5 8 5.328611473086649E-5 3.996458604814987E-5 0.0 5.328611473086649E-5 1.3321528682716622E-5 9 5.328611473086649E-5 6.660764341358311E-5 0.0 6.660764341358311E-5 1.3321528682716622E-5 10-11 5.328611473086649E-5 6.660764341358311E-5 0.0 7.326840775494141E-5 1.3321528682716622E-5 12-13 5.328611473086649E-5 6.660764341358311E-5 0.0 9.325070077901635E-5 2.6643057365433244E-5 14-15 5.99468790722248E-5 6.660764341358311E-5 0.0 9.991146512037467E-5 5.328611473086649E-5 16-17 6.660764341358311E-5 6.660764341358311E-5 0.0 1.3321528682716622E-4 5.328611473086649E-5 18-19 8.658993643765804E-5 7.326840775494141E-5 0.0 1.5985834419259947E-4 7.326840775494141E-5 20-21 1.0657222946173298E-4 7.992917209629974E-5 0.0 4.92896561260515E-4 7.992917209629974E-5 22-23 1.1323299380309128E-4 1.0657222946173298E-4 0.0 0.0019316216589939101 7.992917209629974E-5 24-25 1.3321528682716622E-4 1.198937581444496E-4 0.0 0.004835714911826133 7.992917209629974E-5 26-27 2.1980522326482427E-4 1.3321528682716622E-4 0.0 0.008339276955380606 7.992917209629974E-5 28-29 2.3978751628889918E-4 1.3321528682716622E-4 0.0 0.022686563346666407 7.992917209629974E-5 30-31 2.8641286667840737E-4 1.7317987287531608E-4 0.0 0.06231811117774835 7.992917209629974E-5 32-33 2.9307363101976566E-4 1.7317987287531608E-4 0.0 0.13264912185815075 7.992917209629974E-5 34-35 3.1971668838519894E-4 2.0648369458210763E-4 0.0 0.22584653652243625 7.992917209629974E-5 36-37 3.4635974575063217E-4 2.397875162888992E-4 0.0 0.3678407107515127 7.992917209629974E-5 38-39 3.596812744333488E-4 2.6643057365433243E-4 0.0 0.5896308417900618 7.992917209629974E-5 40-41 3.9298509614014033E-4 2.6643057365433243E-4 0.0 0.8558882355715189 7.992917209629974E-5 42-43 4.5293197521236513E-4 2.6643057365433243E-4 0.0 1.1004048945427825 9.991146512037467E-5 44-45 4.5293197521236513E-4 2.8641286667840737E-4 0.0 1.3759740368734585 1.0657222946173298E-4 46-47 4.5293197521236513E-4 3.063951597024823E-4 0.0 1.6726578021662404 1.198937581444496E-4 48-49 4.7291426823644006E-4 3.063951597024823E-4 0.0 1.9867594662116743 1.3321528682716622E-4 50-51 4.795750325777984E-4 3.4635974575063217E-4 0.0 2.3421179045874814 1.3321528682716622E-4 52-53 4.862357969191567E-4 3.4635974575063217E-4 0.0 2.6898098032063853 1.3321528682716622E-4 54-55 4.92896561260515E-4 3.5302051009199046E-4 0.0 3.0267379066496543 1.3321528682716622E-4 56-57 5.062180899432316E-4 3.596812744333488E-4 0.0 3.4059485421318656 1.3321528682716622E-4 58-59 5.195396186259483E-4 3.596812744333488E-4 0.0 3.8148994903982416 1.3321528682716622E-4 60-61 5.195396186259483E-4 3.596812744333488E-4 0.0 4.22694769408335 1.3321528682716622E-4 62-63 5.328611473086649E-4 3.596812744333488E-4 0.0 4.646495918416827 1.3321528682716622E-4 64-65 5.528434403327398E-4 4.262889178469319E-4 0.0 5.078080143915139 1.3321528682716622E-4 66-67 5.661649690154565E-4 4.262889178469319E-4 0.0 5.539124930095278 1.4653681550988283E-4 68-69 5.728257333568147E-4 4.5293197521236513E-4 0.0 6.03131545033561 1.4653681550988283E-4 70-71 5.728257333568147E-4 4.6625350389508177E-4 0.0 6.577571394734746 1.5319757985124115E-4 72-73 5.928080263808897E-4 4.7291426823644006E-4 0.0 7.106928979999856 1.5985834419259947E-4 74-75 5.99468790722248E-4 4.862357969191567E-4 0.0 7.62546282321026 1.7317987287531608E-4 76-77 5.99468790722248E-4 5.062180899432316E-4 0.0 8.17782002774608 1.865014015580327E-4 78-79 5.99468790722248E-4 5.128788542845899E-4 0.0 8.7435120824933 1.9316216589939102E-4 80-81 5.99468790722248E-4 5.195396186259483E-4 0.0 9.349321920868523 2.3312675194754089E-4 82-83 5.99468790722248E-4 5.195396186259483E-4 0.0 9.992605219428224 2.531090449716158E-4 84-85 5.99468790722248E-4 5.395219116500231E-4 0.0 10.615373363816543 2.531090449716158E-4 86-87 5.99468790722248E-4 5.595042046740981E-4 0.0 11.245588242738503 2.531090449716158E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACTAG 2545 0.0 23.753149 1 TCTAACG 1290 0.0 21.987988 2 CTAACGC 1320 0.0 20.783728 3 GTACAAG 4460 0.0 20.435572 1 TAGTACT 3520 0.0 18.758196 4 GTACAAA 5120 0.0 18.618706 1 GTATAAT 2415 0.0 18.485012 1 GTATAAG 3610 0.0 18.033766 1 GTGATCG 2210 0.0 17.884302 8 GTACATA 2350 0.0 17.809029 1 ACGCCTA 1620 0.0 17.795984 6 GTATCAA 47265 0.0 17.738695 1 TAGGCAT 4090 0.0 17.735632 5 CGGGCGT 2045 0.0 17.280872 6 GTCCTAC 2565 0.0 17.222725 1 TGATCGC 2355 0.0 17.178043 9 CATGGGG 20430 0.0 17.115707 4 GTCTAAC 3655 0.0 17.048376 1 TACATGA 2640 0.0 16.908796 2 CTAGGCA 4595 0.0 16.899597 4 >>END_MODULE