FastQCFastQC Report
Fri 27 May 2016
ERR765546_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765546_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7506646
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254560.3391128341472343No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT235890.3142415400966024No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA234300.31212341703605045No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166050.2212039837765095No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132670.1767367210336014No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG92070.12265131458177195No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG78590.10469389391746993No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG6850.028.5115412
GTATCAA587700.027.138591
AGTACTT399100.022.22722812-13
CAACGCA720150.021.9090165
ATCAACG722700.021.8638843
TATTCGC3450.021.5652359
TATCAAC746100.021.3838372
AACGCAG746750.021.320646
TCAACGC748150.021.1698554
TCTAACG12600.020.298042
CTAACGC12000.020.150423
TAACGCA4850.020.134444
ACGCAGA794050.019.869087
GAGTACT381500.019.82722912-13
TACGTAC11550.019.726767
CGCAGAG810000.019.483578
TACTTTT459750.019.3811314-15
ATCACGC10950.019.1099873
GGTATCA417450.018.6909221
GCAGAGT851200.018.4590729
Produced by FastQC (version 0.11.5)