FastQCFastQC Report
Fri 27 May 2016
ERR765544_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765544_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8389444
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA587610.7004159036045774No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT554890.6614145109020335No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG280130.3339077059218704No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA250740.2988755869876478No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG239790.28582347054226714No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC234360.2793510511542839No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG220380.2626872531719623No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC199580.23789419179626206No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC193350.230468193124598No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT179830.21435270323039288No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT178810.21313688964369987No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154280.18389776485783801No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC146490.17461228658299646No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA112750.13439508029375963No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG111650.13308390877869858No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104830.12495464538531995No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG95700.11407192181031305No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC91250.10876763704483873No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTC64050.022.845355
TCTACAC69950.022.7568243
ATCTACA68300.020.1325172
CTACACT90950.019.3609584
GTATAAG24900.018.3492151
AGTACTT201550.018.04028112-13
GAGTACT188600.017.6339912-13
CACTCTT86400.017.4183037
CATGGGG141700.016.0817574
GTACTTT217700.015.40767514-15
ACTCTTT116050.015.2755098
TACTTTT244350.015.06343414-15
GTATTAG33200.014.8969141
ACACTCT103600.014.5697666
CATGGGT70000.014.259224
GTATTAC12250.014.2269141
TATACCG13900.014.2164465
ATACCGA13400.014.0366176
GTATATA18800.014.0305691
ACTTTTT250300.013.78613616-17
Produced by FastQC (version 0.11.5)