##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765544_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8389444 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.10081418983189 33.0 31.0 34.0 30.0 34.0 2 32.13674779878142 34.0 31.0 34.0 30.0 34.0 3 32.1569459191813 34.0 31.0 34.0 30.0 34.0 4 35.63277387631409 37.0 35.0 37.0 35.0 37.0 5 35.65920602128103 37.0 35.0 37.0 35.0 37.0 6 35.74861421090599 37.0 36.0 37.0 35.0 37.0 7 35.70316829100951 37.0 36.0 37.0 35.0 37.0 8 35.729418302333265 37.0 36.0 37.0 35.0 37.0 9 37.408700624260675 39.0 38.0 39.0 35.0 39.0 10-11 37.332213076337354 39.0 37.5 39.0 34.5 39.0 12-13 37.31939786474527 39.0 37.5 39.0 34.5 39.0 14-15 38.705664523179365 40.5 38.0 41.0 34.5 41.0 16-17 38.660965196263305 40.0 38.0 41.0 34.5 41.0 18-19 38.62665148012192 40.0 38.5 41.0 34.0 41.0 20-21 38.603955041597516 40.0 38.5 41.0 34.0 41.0 22-23 38.50199858298119 40.0 38.0 41.0 34.0 41.0 24-25 38.42488441427108 40.0 38.0 41.0 34.0 41.0 26-27 38.314938510823836 40.0 38.0 41.0 34.0 41.0 28-29 38.19616198642008 40.0 38.0 41.0 34.0 41.0 30-31 38.02141351679563 40.0 38.0 41.0 33.0 41.0 32-33 37.825927796883796 40.0 38.0 41.0 33.0 41.0 34-35 37.68678985162783 40.0 37.5 41.0 33.0 41.0 36-37 37.55107716315885 40.0 37.0 41.0 32.5 41.0 38-39 37.39019713344531 40.0 37.0 41.0 32.0 41.0 40-41 37.19098905720092 40.0 37.0 41.0 31.0 41.0 42-43 36.99662742846844 40.0 36.5 41.0 31.0 41.0 44-45 36.75456377085299 40.0 36.0 41.0 31.0 41.0 46-47 36.43239855942778 39.0 35.5 41.0 30.0 41.0 48-49 36.11320815777542 39.0 35.0 41.0 29.5 41.0 50-51 34.86864528805485 38.0 34.0 40.0 27.5 40.5 52-53 34.83314537888327 38.0 34.0 39.5 27.5 40.0 54-55 35.71659218417812 39.0 35.0 40.5 28.5 41.0 56-57 35.780653759653205 39.0 35.0 41.0 29.0 41.0 58-59 35.58923666455131 38.5 35.0 41.0 29.0 41.0 60-61 35.28740116746712 38.0 35.0 40.0 28.0 41.0 62-63 34.90373778047747 37.0 35.0 40.0 28.0 41.0 64-65 34.596054637232214 37.0 34.0 40.0 27.5 41.0 66-67 34.328013572770736 36.0 34.0 39.0 27.5 41.0 68-69 34.10567833815924 36.0 34.0 39.0 27.0 41.0 70-71 33.76602853538327 35.0 34.0 39.0 26.5 41.0 72-73 33.41770235309992 35.0 34.0 37.5 26.0 40.0 74-75 33.06092406123696 35.0 34.0 37.0 26.0 39.0 76-77 32.72099593250756 35.0 34.0 36.5 26.0 39.0 78-79 32.41873674822789 35.0 34.0 36.0 25.5 38.0 80-81 32.11982373325336 35.0 33.0 36.0 25.0 37.0 82-83 31.875966333406602 35.0 33.0 35.0 24.5 37.0 84-85 31.643454023890023 35.0 33.0 35.0 24.0 36.0 86-87 31.40133452228777 35.0 33.0 35.0 23.0 36.0 88-89 31.207497242963896 35.0 33.0 35.0 21.5 36.0 90-91 30.98030995856221 35.0 33.0 35.0 19.5 35.5 92-93 30.73092799713545 35.0 33.0 35.0 18.0 35.0 94-95 30.44803803446331 35.0 32.0 35.0 9.0 35.0 96-97 30.17865099284291 35.0 32.0 35.0 2.0 35.0 98-99 29.88136424773799 35.0 32.0 35.0 2.0 35.0 100 29.348329043021206 34.0 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5223359041913227 1101 2 0.604051837907214 1101 3 0.6076016146474217 1101 4 0.4700595892465884 1101 5 0.5321268911928314 1101 6 0.507674040382085 1101 7 0.5580757071312021 1101 8 0.5189765803685873 1101 9 0.6134098558554228 1101 10-11 0.6009144972852809 1101 12-13 0.5909478090543914 1101 14-15 0.8297751707652807 1101 16-17 0.7881493311583512 1101 18-19 0.7932414194070816 1101 20-21 0.816947026164307 1101 22-23 0.7311572142548002 1101 24-25 0.8312451415895694 1101 26-27 0.8277932949786617 1101 28-29 0.8637373103408876 1101 30-31 0.8907292555962627 1101 32-33 0.7795979587077824 1101 34-35 0.9391073770008234 1101 36-37 0.9607473305918788 1101 38-39 0.898825925545367 1101 40-41 0.9831498066187052 1101 42-43 0.8527136650434244 1101 44-45 0.8709073854406597 1101 46-47 0.8565740829744826 1101 48-49 1.0004827254129722 1101 50-51 0.9275183528640198 1101 52-53 0.9723093032336152 1101 54-55 1.0969652885855936 1101 56-57 1.1205975685742544 1101 58-59 1.0575063289193523 1101 60-61 1.0678435658865197 1101 62-63 1.0803775479495599 1101 64-65 1.0697674798044545 1101 66-67 1.0327002750485832 1101 68-69 1.0390858831614054 1101 70-71 0.997955537230709 1101 72-73 0.9651565514375093 1101 74-75 0.9053297851689308 1101 76-77 0.8854498626969587 1101 78-79 0.9363689416295031 1101 80-81 1.0038356233311703 1101 82-83 0.9810893512204721 1101 84-85 0.8972045443541212 1101 86-87 0.9847324124998309 1101 88-89 1.0094244300907391 1101 90-91 1.0030777160722018 1101 92-93 0.9933824077313353 1101 94-95 1.0089623155920968 1101 96-97 0.9946082910673866 1101 98-99 0.9868949542364582 1101 100 1.059948367651188 1102 1 0.5162104113897144 1102 2 0.6134424487490264 1102 3 0.6144182858982887 1102 4 0.5227030300883655 1102 5 0.509126537554458 1102 6 0.4992010079866702 1102 7 0.5250030660003162 1102 8 0.5175756961535996 1102 9 0.631014592942563 1102 10-11 0.6181457351029138 1102 12-13 0.6729336254172509 1102 14-15 0.8848005981973799 1102 16-17 0.8537408657464454 1102 18-19 0.8245180539129038 1102 20-21 0.8000925382314747 1102 22-23 0.8532702451737464 1102 24-25 0.7949059654836148 1102 26-27 0.8457969289998601 1102 28-29 0.8782562847658113 1102 30-31 0.9373192053605592 1102 32-33 0.9621896516591875 1102 34-35 0.9868178960157366 1102 36-37 0.9968913507099728 1102 38-39 0.9397464926527164 1102 40-41 1.0020760557539603 1102 42-43 0.9784304852510601 1102 44-45 1.103270880435545 1102 46-47 1.11571575383838 1102 48-49 1.0428056889263075 1102 50-51 1.056736562025037 1102 52-53 1.0770772652645277 1102 54-55 1.1749602304853042 1102 56-57 1.0897742579714134 1102 58-59 1.1002833188495842 1102 60-61 1.1233135774720395 1102 62-63 1.153373222130746 1102 64-65 1.1251862316872447 1102 66-67 1.0965235939863334 1102 68-69 1.0387151344125343 1102 70-71 0.9566355135595686 1102 72-73 0.929767209883579 1102 74-75 0.8906823816723204 1102 76-77 0.882940732992644 1102 78-79 0.8944693677816034 1102 80-81 0.9144854129937343 1102 82-83 0.9525338627411095 1102 84-85 0.9398786933894705 1102 86-87 0.9454743624641679 1102 88-89 0.8800803280523795 1102 90-91 0.8974623674526363 1102 92-93 0.9321481145734616 1102 94-95 0.7750226770176525 1102 96-97 0.9219902955182278 1102 98-99 1.0300614821863903 1102 100 1.0334541145723328 1103 1 0.512812100495708 1103 2 0.5620566674986804 1103 3 0.6460950796583163 1103 4 0.5223120998821145 1103 5 0.5480173268909567 1103 6 0.48840395138808645 1103 7 0.5645509961022981 1103 8 0.5762077288265104 1103 9 0.6677226331669743 1103 10-11 0.6364155439415455 1103 12-13 0.6761934846362365 1103 14-15 0.7994021142665062 1103 16-17 0.835976280180823 1103 18-19 0.7939240272341621 1103 20-21 0.7781685235987723 1103 22-23 0.8337643763401275 1103 24-25 0.8546314802128236 1103 26-27 0.8201167277446757 1103 28-29 0.8722714720782179 1103 30-31 0.9221759286191542 1103 32-33 0.9228394272658704 1103 34-35 0.9409109942851472 1103 36-37 0.934583371713984 1103 38-39 0.9336927092460598 1103 40-41 0.9909617870898231 1103 42-43 0.9792278080602088 1103 44-45 0.988537257376386 1103 46-47 1.0033248724373962 1103 48-49 1.0230642681161797 1103 50-51 0.9124426744413228 1103 52-53 0.9177256654499857 1103 54-55 0.9980619828163171 1103 56-57 1.0299425030448504 1103 58-59 1.0550059607231788 1103 60-61 1.0578736090623622 1103 62-63 1.088001758571643 1103 64-65 1.0682884055183095 1103 66-67 0.9915059908131028 1103 68-69 0.9398056437842541 1103 70-71 0.9244012970726985 1103 72-73 0.875321648833733 1103 74-75 0.9212299102400436 1103 76-77 0.9006717316593722 1103 78-79 0.9201706218551706 1103 80-81 0.8741396942141364 1103 82-83 0.8368003084530109 1103 84-85 0.8314777536661957 1103 86-87 0.8397435590185154 1103 88-89 0.8608912719684696 1103 90-91 0.8788195310678653 1103 92-93 0.8470149094648818 1103 94-95 0.902629266616362 1103 96-97 0.9577582797053239 1103 98-99 0.9738663853412106 1103 100 0.9603055757355392 1104 1 0.40676634354428387 1104 2 0.4914543831090157 1104 3 0.5148070938098215 1104 4 0.437089457711231 1104 5 0.4865457244914708 1104 6 0.45653068208051195 1104 7 0.4747005512675244 1104 8 0.48179495838755315 1104 9 0.5720854965367579 1104 10-11 0.591286224594775 1104 12-13 0.5440559613506579 1104 14-15 0.6875167297448357 1104 16-17 0.6896279244441175 1104 18-19 0.6856811859777707 1104 20-21 0.6393134925893875 1104 22-23 0.6468909584168543 1104 24-25 0.6377219786338344 1104 26-27 0.6501703339544349 1104 28-29 0.6833298484405859 1104 30-31 0.6442832983872577 1104 32-33 0.6429677509462266 1104 34-35 0.6918506018402439 1104 36-37 0.6874831106320372 1104 38-39 0.6406988447050139 1104 40-41 0.6749175793444238 1104 42-43 0.7191641367105532 1104 44-45 0.7473490226171648 1104 46-47 0.721934026514127 1104 48-49 0.7208272514201965 1104 50-51 0.5934276213357847 1104 52-53 0.6457849763094146 1104 54-55 0.7470381792813257 1104 56-57 0.7468578591815103 1104 58-59 0.7919432873112768 1104 60-61 0.7696782985904704 1104 62-63 0.7271304265156573 1104 64-65 0.7398877132472137 1104 66-67 0.6831029011104306 1104 68-69 0.7122880361531045 1104 70-71 0.6289341070908137 1104 72-73 0.6328895849055627 1104 74-75 0.5814381284647396 1104 76-77 0.568985359804735 1104 78-79 0.5302798334397139 1104 80-81 0.5450347515224294 1104 82-83 0.544097076686267 1104 84-85 0.5109293840743554 1104 86-87 0.4785574123195886 1104 88-89 0.4554696245711298 1104 90-91 0.4813594163678765 1104 92-93 0.47033536120489927 1104 94-95 0.4615946190307767 1104 96-97 0.487130145191788 1104 98-99 0.5432047901704422 1104 100 0.5961820858442834 1105 1 0.24278786431820976 1105 2 0.3553184272098946 1105 3 0.37836167559554923 1105 4 0.3249688538286577 1105 5 0.38711155270497954 1105 6 0.3193083115078039 1105 7 0.3725145477515639 1105 8 0.37646837008558975 1105 9 0.49477454910358887 1105 10-11 0.45630916465912463 1105 12-13 0.4898198136915042 1105 14-15 0.5586866404070818 1105 16-17 0.5561516953048127 1105 18-19 0.6115708537936939 1105 20-21 0.5897503158741912 1105 22-23 0.54079504918748 1105 24-25 0.5189064379877024 1105 26-27 0.4571905219620973 1105 28-29 0.5119580699882036 1105 30-31 0.5391526094387942 1105 32-33 0.53497554358907 1105 34-35 0.5265810263847115 1105 36-37 0.5551888361451773 1105 38-39 0.4574015881975484 1105 40-41 0.5358589411070156 1105 42-43 0.6051469837801235 1105 44-45 0.5643553361294664 1105 46-47 0.5672619303294297 1105 48-49 0.6550415645342156 1105 50-51 0.5728490003519795 1105 52-53 0.5786165208451237 1105 54-55 0.6191072576797794 1105 56-57 0.6080907121909576 1105 58-59 0.5801011302930092 1105 60-61 0.5542497237404902 1105 62-63 0.5646087026781785 1105 64-65 0.5164029689191025 1105 66-67 0.48308734233575024 1105 68-69 0.43280980384102463 1105 70-71 0.4117713976960715 1105 72-73 0.39284634843608757 1105 74-75 0.46726512821504684 1105 76-77 0.4401192071965667 1105 78-79 0.4291853408704398 1105 80-81 0.4005033995185485 1105 82-83 0.40399405721280957 1105 84-85 0.39382228894949733 1105 86-87 0.38159939763239237 1105 88-89 0.4611203271897182 1105 90-91 0.48750988254188954 1105 92-93 0.44670672027468683 1105 94-95 0.4368589533717646 1105 96-97 0.4647065176029912 1105 98-99 0.45316208928552015 1105 100 0.5109080429732593 1106 1 0.13008142115921828 1106 2 0.22483953701125614 1106 3 0.2544428974906552 1106 4 0.23688727657707176 1106 5 0.31839666309450365 1106 6 0.2866131485009831 1106 7 0.1861355986644071 1106 8 0.2918581417546591 1106 9 0.31338881277505237 1106 10-11 0.3377623534434946 1106 12-13 0.3197412508265316 1106 14-15 0.41537130564751834 1106 16-17 0.3876505427240531 1106 18-19 0.34989680901139053 1106 20-21 0.358114395760019 1106 22-23 0.3790775063542853 1106 24-25 0.38420183429279575 1106 26-27 0.3637065888596638 1106 28-29 0.34885179400609445 1106 30-31 0.39011872644255874 1106 32-33 0.3602641559324553 1106 34-35 0.3232822810064704 1106 36-37 0.3727405980498162 1106 38-39 0.37562950009800034 1106 40-41 0.3812155629705387 1106 42-43 0.2845295763909803 1106 44-45 0.33662526549284877 1106 46-47 0.30631094925232816 1106 48-49 0.28570544423686073 1106 50-51 0.280108397728398 1106 52-53 0.32242525587255955 1106 54-55 0.35807223700529534 1106 56-57 0.37923269833061823 1106 58-59 0.37189659541522957 1106 60-61 0.37140714907247485 1106 62-63 0.38918345039660807 1106 64-65 0.34062080032344966 1106 66-67 0.280528930935958 1106 68-69 0.2945279018187179 1106 70-71 0.31443580969750684 1106 72-73 0.28076573870377075 1106 74-75 0.3118253657042658 1106 76-77 0.33280592080819815 1106 78-79 0.29734569072601147 1106 80-81 0.3301819505383108 1106 82-83 0.32450887645911664 1106 84-85 0.3880383872471249 1106 86-87 0.3733434150985495 1106 88-89 0.34356697664392044 1106 90-91 0.3461605112174304 1106 92-93 0.3861877632020878 1106 94-95 0.4148144294714413 1106 96-97 0.4389221676254422 1106 98-99 0.4287163654667694 1106 100 0.49956773682242783 1107 1 0.20573095964931554 1107 2 0.25134020549324987 1107 3 0.26657198587029285 1107 4 0.24221866677915926 1107 5 0.3118904196217258 1107 6 0.2352220864202934 1107 7 0.31563656657180417 1107 8 0.24846285400774804 1107 9 0.3285733137313471 1107 10-11 0.3605706730197653 1107 12-13 0.36959307008866205 1107 14-15 0.38923831537363895 1107 16-17 0.37964787643714004 1107 18-19 0.36975406167960756 1107 20-21 0.3508986781515162 1107 22-23 0.32965436657671177 1107 24-25 0.4105958572913906 1107 26-27 0.3708561661919134 1107 28-29 0.37167436943759213 1107 30-31 0.4217203841370676 1107 32-33 0.41787412697051707 1107 34-35 0.39507965968206094 1107 36-37 0.43902209942584847 1107 38-39 0.43603706473572146 1107 40-41 0.4169281808107641 1107 42-43 0.4408041041592199 1107 44-45 0.42910862423526197 1107 46-47 0.47032413655516336 1107 48-49 0.5208317890947711 1107 50-51 0.3916194876675618 1107 52-53 0.5368625792147554 1107 54-55 0.5487257971747752 1107 56-57 0.5675140182684899 1107 58-59 0.4941640090397641 1107 60-61 0.5432106181370742 1107 62-63 0.5309701338097241 1107 64-65 0.5184706023408623 1107 66-67 0.5385100711062307 1107 68-69 0.5068115572297529 1107 70-71 0.531448116553328 1107 72-73 0.46203741043165536 1107 74-75 0.44639045087494367 1107 76-77 0.49279650729917535 1107 78-79 0.5179395059022553 1107 80-81 0.5631811390108226 1107 82-83 0.6083403469299888 1107 84-85 0.6383634514602825 1107 86-87 0.577270390060761 1107 88-89 0.5802999256709569 1107 90-91 0.6190498599361014 1107 92-93 0.6726323401751308 1107 94-95 0.572999294277917 1107 96-97 0.5867105885598676 1107 98-99 0.6059706502951627 1107 100 0.6416040138163197 1108 1 0.13532388281707597 1108 2 0.12369235509911647 1108 3 0.09865556340134418 1108 4 0.21295971149406512 1108 5 0.21914817113149354 1108 6 0.19268888906114512 1108 7 0.2853434318891672 1108 8 0.23686861693742856 1108 9 0.28234313116158205 1108 10-11 0.2104195728134428 1108 12-13 0.27432161564539825 1108 14-15 0.2987935256128722 1108 16-17 0.31204830271423134 1108 18-19 0.3322038884904188 1108 20-21 0.33395564626135155 1108 22-23 0.3221330933139228 1108 24-25 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-0.69162092031846 2316 92-93 -0.6649002314825161 2316 94-95 -0.5519340993740123 2316 96-97 -0.5264291608790543 2316 98-99 -0.6462627651323913 2316 100 -0.7187515568481118 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 24061.0 3 4090.0 4 4020.0 5 4621.0 6 5528.0 7 6383.0 8 7768.0 9 9529.0 10 11791.0 11 15633.0 12 20648.0 13 26234.0 14 26832.0 15 31847.0 16 40652.0 17 53484.0 18 51989.0 19 34163.0 20 31577.0 21 32718.0 22 36262.0 23 42644.0 24 49523.0 25 56553.0 26 65296.0 27 76485.0 28 91478.0 29 110194.0 30 134724.0 31 167408.0 32 214422.0 33 287374.0 34 413033.0 35 657529.0 36 1182317.0 37 2059251.0 38 1884697.0 39 416344.0 40 342.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.615372149322276 18.536329694926078 13.08892207501151 24.759376080740132 2 15.671046355162863 21.485251228201907 37.09476635180813 25.748936064827095 3 18.79290480605619 25.922833597823107 29.4528657206775 25.831395875443203 4 13.644645474876965 17.690529166190068 35.389876412867075 33.27494894606589 5 14.380718198976405 36.221909820171675 33.682713233330134 15.714658747521781 6 31.764853328749858 34.808890512203504 17.941493831404454 15.484762327642182 7 26.581714965989157 31.459201193441704 22.45146688703061 19.507616953538527 8 26.816322886781908 31.809523513990335 21.322353287151227 20.05180031207653 9 27.117627335716975 14.745991506950668 20.064234792404537 38.072146364927825 10-11 25.163737811900354 25.143801942331823 27.056636085393134 22.635824160374693 12-13 26.609711441673454 23.160083174985 26.886883740666644 23.343321642674894 14-15 24.77631803409848 23.579748295786295 24.86393568283211 26.779997987283117 16-17 23.603058030935884 26.370172471240323 26.319313110978506 23.707456386845294 18-19 24.276289140281055 25.67745577831561 27.593892920531154 22.452362160872177 20-21 24.705381851683235 25.16530402384296 26.85700986299633 23.272304261477476 22-23 23.907872553924708 25.453432703293654 25.861491243083435 24.777203499698203 24-25 23.857098709855222 26.037337585574605 25.622569724226835 24.482993980343334 26-27 24.310824942048853 25.304233066409015 25.78726275969958 24.59767923184255 28-29 23.80495492601667 24.90089503148504 27.217786377476074 24.07636366502222 30-31 24.680674845361526 24.997761589545586 26.614016385702104 23.707547179390783 32-33 23.22312094811799 25.53774827949937 26.574713508722258 24.664417263660372 34-35 23.39343314294035 25.71829437647442 26.929097064614062 23.959175415971167 36-37 24.169536949108743 25.640451436932814 26.149672421230264 24.04033919272818 38-39 24.432182316289577 24.862077900635008 26.17465225402003 24.53108752905538 40-41 23.901953800689295 25.437005795925238 26.246414417333458 24.41462598605201 42-43 24.97070602447322 25.076841888160295 25.953221728942783 23.999230358423702 44-45 24.656981889245095 24.979707712310184 26.178596233049838 24.184714165394883 46-47 23.972863024668452 25.071232859215552 27.013695438202273 23.942208677913715 48-49 24.426624452052856 25.53963826319407 26.486026444344652 23.547710840408424 50-51 23.5799770502427 25.903378763360468 25.87492292713473 24.641721259262102 52-53 23.427125231175253 25.935351358936977 25.247010562999595 25.390512846888175 54-55 23.961399730869154 25.584569433880013 25.21827000687885 25.235760828371983 56-57 24.251923709951427 24.832628749879742 25.432246873779352 25.483200666389482 58-59 24.005616169470276 24.72243433214134 26.512713647790616 24.75923585059777 60-61 23.995294035679958 25.057148368869054 26.062161534378674 24.885396061072314 62-63 23.058162508156524 25.290714704246714 26.315850700014447 25.33527208758231 64-65 23.004364176701277 25.192245655854638 26.368065793712002 25.435324373732083 66-67 23.948576476436962 25.04701308443598 25.791747114857376 25.21266332426968 68-69 24.048449522113778 24.44819279782055 25.878382622635872 25.6249750574298 70-71 23.2782043977275 24.786758982391476 26.134920326128547 25.800116293752478 72-73 24.044177801366903 24.45012326392806 26.153631824118712 25.352067110586322 74-75 24.13811821277162 24.18008053756682 26.316767242084577 25.365034007576988 76-77 23.41814489444864 24.742694791720037 26.769302115493872 25.069858198337446 78-79 23.527266020085097 24.623111948388082 26.753894292986452 25.09572773854037 80-81 23.07093885396142 24.615031279621984 26.64269765338707 25.67133221302953 82-83 23.052303174412582 24.927843408918225 25.94281588664821 26.077037530020984 84-85 23.2768307215722 24.73473528387997 25.89030310739989 26.098130887147935 86-87 23.31800101278809 24.06828450867658 26.342311389710755 26.271403088824574 88-89 23.394899203154953 23.63438371510094 26.854339476309853 26.116377605434256 90-91 23.726166675463638 23.98990863966896 26.46805732058895 25.81586736427845 92-93 23.06847162096222 24.14265277499284 26.69973632292635 26.08913928111859 94-95 22.765276088596032 24.14926388354899 27.009048831251686 26.076411196603296 96-97 23.58671232390083 23.91219295264826 26.407725847039654 26.093368876411255 98-99 23.57126306351247 23.584107982030993 26.39456059878978 26.45006835566676 100 23.47609447221726 23.702268186600612 26.68103176471137 26.140605576470755 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1798.0 1 2344.5 2 1996.5 3 1024.0 4 836.0 5 828.0 6 1066.0 7 1715.5 8 2504.5 9 2640.5 10 2209.5 11 1992.5 12 2175.5 13 2333.5 14 2555.0 15 2863.0 16 3137.0 17 3564.0 18 4126.5 19 4625.0 20 5298.0 21 6329.0 22 7625.0 23 9352.0 24 11509.5 25 14315.0 26 18341.5 27 23754.0 28 30356.5 29 40250.0 30 51600.5 31 61155.5 32 71517.5 33 83339.0 34 95760.5 35 107041.5 36 118356.0 37 130894.5 38 141098.5 39 152227.5 40 163070.5 41 172174.0 42 183519.0 43 204154.0 44 231333.0 45 256305.0 46 287066.5 47 323705.0 48 399998.5 49 480376.0 50 546601.0 51 515830.0 52 409232.0 53 368102.0 54 343521.5 55 315313.0 56 292048.5 57 265430.0 58 244879.5 59 223342.0 60 190671.0 61 157027.0 62 127510.5 63 99984.0 64 75047.0 65 59340.0 66 46553.0 67 34747.5 68 26901.0 69 21708.5 70 18325.5 71 15498.0 72 12695.5 73 12174.5 74 10029.0 75 7834.0 76 6103.5 77 4173.0 78 3035.5 79 2167.5 80 1507.5 81 1169.5 82 956.0 83 759.0 84 525.0 85 335.5 86 242.5 87 176.5 88 125.5 89 100.5 90 85.5 91 69.5 92 62.5 93 54.5 94 42.5 95 36.5 96 28.5 97 26.5 98 25.0 99 17.5 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11645587001951499 2 0.3705489898973043 3 0.3795603141280876 4 0.3768545329106434 5 0.3048831364748367 6 0.13736309581421605 7 0.1549327941160344 8 0.12789882142368433 9 0.21046686764939368 10-11 0.20978148253924814 12-13 0.22239852843644942 14-15 0.15146414947164558 16-17 0.1363439579547822 18-19 0.15575525624820907 20-21 0.15657175850986071 22-23 0.16458182449277925 24-25 0.1773657467646247 26-27 0.1702854205832949 28-29 0.17867691827968576 30-31 0.22109331679191135 32-33 0.21853653233754228 34-35 0.1330123903324225 36-37 0.09201444100467207 38-39 0.08553606174616578 40-41 0.08450500414568594 42-43 0.07534468315182746 44-45 0.09751540149740555 46-47 0.14500960969523127 48-49 0.2250804701718016 50-51 0.17955301924656747 52-53 0.11249851599223978 54-55 0.06015297318868807 56-57 0.07579167344105282 58-59 0.11150917748542095 60-61 0.08719290575156113 62-63 0.09675253807046093 64-65 0.10519767460155881 66-67 0.10170519047507796 68-69 0.09165684877329178 70-71 0.12708827903255568 72-73 0.15141647050746151 74-75 0.19616317839418201 76-77 0.19561487030606556 78-79 0.15595789184599124 80-81 0.09284286300736973 82-83 0.1327620757704563 84-85 0.16113701933048244 86-87 0.17334879403211942 88-89 0.16135753453983365 90-91 0.18296802505624926 92-93 0.10189590633181411 94-95 0.34138734342824145 96-97 0.34430172011399085 98-99 0.3820694196182727 100 0.3832315943702586 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8389444.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.18139946152339 #Duplication Level Percentage of deduplicated Percentage of total 1 77.40457527891407 34.19842460547261 2 12.764909776404828 11.279431558432941 3 4.016539087011576 5.323689535682427 4 1.770050577274035 3.128132464865768 5 0.9748082412828939 2.1534196153252303 6 0.5903812605754265 1.565032218484839 7 0.41913379359750874 1.296254229392866 8 0.28228289386700367 0.9977322636074322 9 0.2201891487850491 0.8755438265608557 >10 1.3124408113841604 10.971768149815613 >50 0.12585839835850549 3.8603049198527692 >100 0.09769810079117267 8.77964781599671 >500 0.0132557449030191 3.995671574534559 >1k 0.006926228099874003 5.492335547691556 >5k 5.160718933454752E-4 1.5830940504541098 >10k+ 4.345868575540843E-4 4.499517623829723 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 58761 0.7004159036045774 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 55489 0.6614145109020335 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 28013 0.3339077059218704 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 25074 0.2988755869876478 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 23979 0.28582347054226714 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 23436 0.2793510511542839 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 22038 0.2626872531719623 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 19958 0.23789419179626206 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 19335 0.230468193124598 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 17983 0.21435270323039288 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 17881 0.21313688964369987 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15428 0.18389776485783801 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 14649 0.17461228658299646 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 11275 0.13439508029375963 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 11165 0.13308390877869858 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10483 0.12495464538531995 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 9570 0.11407192181031305 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 9125 0.10876763704483873 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 4.76789641840389E-5 0.0 0.0 0.0 0.0 2 9.53579283680778E-5 0.0 0.0 0.0 0.0 3 1.1919741046009723E-4 0.0 0.0 1.1919741046009724E-5 0.0 4 1.430368925521167E-4 1.1919741046009724E-5 0.0 4.76789641840389E-5 0.0 5 5.840673112544765E-4 3.575922313802917E-5 0.0 5.9598705230048615E-5 1.1919741046009724E-5 6 0.0012277333277390015 4.76789641840389E-5 0.0 5.9598705230048615E-5 1.1919741046009724E-5 7 0.0014422886665671766 4.76789641840389E-5 0.0 5.9598705230048615E-5 1.1919741046009724E-5 8 0.001597245300165303 4.76789641840389E-5 0.0 7.151844627605835E-5 1.1919741046009724E-5 9 0.00172836245167141 4.76789641840389E-5 0.0 8.343818732206806E-5 4.76789641840389E-5 10-11 0.002848818109996324 4.76789641840389E-5 0.0 1.1323753993709238E-4 6.555857575305348E-5 12-13 0.005769154666268705 5.3638834707043756E-5 0.0 1.4899676307512154E-4 7.151844627605835E-5 14-15 0.008987484748691332 1.0727766941408751E-4 0.0 2.0859546830517017E-4 1.1919741046009723E-4 16-17 0.010316535875321416 1.1919741046009723E-4 0.0 2.801139145812285E-4 1.3111715150610696E-4 18-19 0.011150917748542096 1.251572809831021E-4 0.0 3.3375274928827226E-4 1.4899676307512154E-4 20-21 0.014476525500378808 1.3111715150610696E-4 0.0 5.542679586394521E-4 1.549566335981264E-4 22-23 0.015662539734456776 1.3111715150610696E-4 0.0 0.0012694524214000356 1.549566335981264E-4 24-25 0.02290974229043069 1.370770220291118E-4 0.0 0.003367326845497747 1.549566335981264E-4 26-27 0.04533077519797497 1.430368925521167E-4 0.0 0.007861069219843413 1.6091650412113128E-4 28-29 0.05026554799102301 1.4899676307512154E-4 5.959870523004862E-6 0.02510297464289648 1.6687637464413613E-4 30-31 0.05120124766313477 1.549566335981264E-4 1.1919741046009724E-5 0.07542216146862653 1.6687637464413613E-4 32-33 0.051999870313217414 1.9667572725916044E-4 1.1919741046009724E-5 0.17108404323337756 1.907158567361556E-4 34-35 0.05257797775394889 2.0859546830517015E-4 1.1919741046009724E-5 0.3044599856677034 1.907158567361556E-4 36-37 0.053579236001813704 2.1455533882817503E-4 1.1919741046009724E-5 0.5032216676099155 1.907158567361556E-4 38-39 0.054503015932879456 2.1455533882817503E-4 1.1919741046009724E-5 0.7863870358989224 1.907158567361556E-4 40-41 0.05554599327440531 2.1455533882817503E-4 1.1919741046009724E-5 1.1494623481603785 1.907158567361556E-4 42-43 0.05782266381419317 2.1455533882817503E-4 1.1919741046009724E-5 1.4434210419665474 1.907158567361556E-4 44-45 0.06057016412529841 2.1455533882817503E-4 1.1919741046009724E-5 1.7720959815692197 2.0859546830517015E-4 46-47 0.06196477382768155 2.1455533882817503E-4 1.1919741046009724E-5 2.153575374005715 2.1455533882817503E-4 48-49 0.06283491492404025 2.1455533882817503E-4 1.1919741046009724E-5 2.515583869443553 2.383948209201945E-4 50-51 0.06397325019393418 2.503145619662042E-4 1.1919741046009724E-5 2.8963480774172874 2.503145619662042E-4 52-53 0.06447983918838959 2.622343030122139E-4 1.1919741046009724E-5 3.319528683903248 2.622343030122139E-4 54-55 0.06604728513593988 2.741540440582236E-4 1.1919741046009724E-5 3.730557114392801 2.801139145812285E-4 56-57 0.06959936796765077 2.741540440582236E-4 1.1919741046009724E-5 4.174627067061893 2.979935261502431E-4 58-59 0.07095225857637288 2.741540440582236E-4 1.1919741046009724E-5 4.637297775633284 2.979935261502431E-4 60-61 0.07176280096750154 2.801139145812285E-4 1.1919741046009724E-5 5.078655987214409 3.099132671962528E-4 62-63 0.07228130970300296 2.979935261502431E-4 1.1919741046009724E-5 5.525664156051343 3.099132671962528E-4 64-65 0.07266870128699827 2.979935261502431E-4 1.1919741046009724E-5 6.009849997210781 3.3375274928827226E-4 66-67 0.07316933041093068 2.979935261502431E-4 1.1919741046009724E-5 6.534878831064371 3.397126198112771E-4 68-69 0.07374743785166216 3.099132671962528E-4 1.1919741046009724E-5 7.1223253889053915 3.4567249033428196E-4 70-71 0.07468313752377392 3.3375274928827226E-4 1.1919741046009724E-5 7.782863798840543 3.516323608572868E-4 72-73 0.07702536663931484 3.4567249033428196E-4 1.1919741046009724E-5 8.374434587083483 3.575922313802917E-4 74-75 0.07911132132236653 3.516323608572868E-4 1.1919741046009724E-5 8.959628313866807 3.575922313802917E-4 76-77 0.0799397433250642 3.814317134723112E-4 1.1919741046009724E-5 9.598502594450835 3.575922313802917E-4 78-79 0.08048209154265765 3.933514545183209E-4 1.1919741046009724E-5 10.254988292430344 3.575922313802917E-4 80-81 0.08138203199163138 4.231508071333452E-4 1.1919741046009724E-5 10.964046008293279 3.575922313802917E-4 82-83 0.08173962422301168 4.2911067765635005E-4 1.1919741046009724E-5 11.733459332942683 3.575922313802917E-4 84-85 0.08325939120637792 4.4699028922536466E-4 1.1919741046009724E-5 12.484617574180124 3.575922313802917E-4 86-87 0.08647772128880055 4.767896418403889E-4 1.1919741046009724E-5 13.24276674354105 3.695119724263015E-4 88 0.08712138730528507 4.767896418403889E-4 1.1919741046009724E-5 13.846245353088953 3.814317134723112E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACACTC 6405 0.0 22.84535 5 TCTACAC 6995 0.0 22.756824 3 ATCTACA 6830 0.0 20.132517 2 CTACACT 9095 0.0 19.360958 4 GTATAAG 2490 0.0 18.349215 1 AGTACTT 20155 0.0 18.040281 12-13 GAGTACT 18860 0.0 17.63399 12-13 CACTCTT 8640 0.0 17.418303 7 CATGGGG 14170 0.0 16.081757 4 GTACTTT 21770 0.0 15.407675 14-15 ACTCTTT 11605 0.0 15.275509 8 TACTTTT 24435 0.0 15.063434 14-15 GTATTAG 3320 0.0 14.896914 1 ACACTCT 10360 0.0 14.569766 6 CATGGGT 7000 0.0 14.25922 4 GTATTAC 1225 0.0 14.226914 1 TATACCG 1390 0.0 14.216446 5 ATACCGA 1340 0.0 14.036617 6 GTATATA 1880 0.0 14.030569 1 ACTTTTT 25030 0.0 13.786136 16-17 >>END_MODULE