FastQCFastQC Report
Fri 27 May 2016
ERR765544_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765544_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8389444
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA590550.7039203074721043No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT546170.6510204967099131No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG303200.36140654851501486No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG259090.30882857076106596No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA258560.3081968244856274No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG254690.30358388470082165No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC233320.2781113980854989No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT206280.24588041829708857No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC198330.23640422416551085No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC197750.23571287918484227No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT182960.2180835821777939No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC165310.19704523923158673No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145750.17373022574559172No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG115730.13794716312547053No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA115290.1374226945194461No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103910.12385802920908703No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC99110.11813655350700236No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG96880.1154784512537422No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT92020.10968545710538147No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC90160.10746838527082365No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC85310.10168731086350896No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAAC7750.021.2437171
TCCGATC19750.020.7085443
AGTACTT192300.018.25441412-13
GAGTACT176650.017.42425512-13
GTATAAG24550.016.2866461
CGATCTC22900.016.2108295
TACTTTT225550.015.8458414-15
GTATATA19550.015.6397851
GTATAAA45250.015.5932581
GTACTTT203700.015.29612514-15
CTAACGC11650.014.930463
ACTGCGC21850.014.8394028
TCTCGCG10200.014.7424138
CCGATCT29150.014.5091124
TCTACAC45250.014.4408793
TCTAACG12150.013.9376772
ATATACG27750.013.8855936
ACTTTTT230700.013.659862516-17
GTATTAG33300.013.2784631
CATGGGG157900.013.2438664
Produced by FastQC (version 0.11.5)