##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765544_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8389444 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.52509689557496 34.0 31.0 34.0 31.0 34.0 2 32.67368516912444 34.0 31.0 34.0 31.0 34.0 3 32.730006541553884 34.0 31.0 34.0 31.0 34.0 4 36.22276804040887 37.0 37.0 37.0 35.0 37.0 5 36.162230774768865 37.0 35.0 37.0 35.0 37.0 6 36.20354805395924 37.0 37.0 37.0 35.0 37.0 7 36.163362792576 37.0 37.0 37.0 35.0 37.0 8 36.162265342017896 37.0 37.0 37.0 35.0 37.0 9 37.91062852317746 39.0 38.0 39.0 35.0 39.0 10-11 37.86152592472159 39.0 38.0 39.0 35.0 39.0 12-13 37.82774585538684 39.0 38.0 39.0 35.0 39.0 14-15 39.23503279835946 41.0 39.0 41.0 36.0 41.0 16-17 39.21762353977212 40.0 39.0 41.0 36.0 41.0 18-19 39.23462436843252 41.0 39.0 41.0 36.0 41.0 20-21 39.20201142054229 40.5 39.0 41.0 36.0 41.0 22-23 39.128783921795055 40.0 39.0 41.0 36.0 41.0 24-25 39.066480746519076 40.0 39.0 41.0 35.0 41.0 26-27 38.953497454658496 40.0 38.0 41.0 35.0 41.0 28-29 38.83972126162354 40.0 38.0 41.0 35.0 41.0 30-31 38.71105707362729 40.0 38.0 41.0 35.0 41.0 32-33 38.52934187295368 40.0 38.0 41.0 34.0 41.0 34-35 38.428857859948764 40.0 38.0 41.0 34.0 41.0 36-37 38.320630961956475 40.0 38.0 41.0 34.0 41.0 38-39 38.18397583916169 40.0 38.0 41.0 34.0 41.0 40-41 38.01898492915621 40.0 37.5 41.0 33.0 41.0 42-43 37.81992567087879 40.0 37.0 41.0 33.0 41.0 44-45 37.581749517608074 40.0 37.0 41.0 33.0 41.0 46-47 37.317842696130995 40.0 36.5 41.0 32.5 41.0 48-49 37.076658238615096 40.0 36.0 41.0 32.0 41.0 50-51 36.8221913156581 39.0 36.0 41.0 31.0 41.0 52-53 36.69649198445094 39.0 35.0 41.0 31.0 41.0 54-55 36.698538127198894 39.0 35.0 41.0 31.0 41.0 56-57 36.54201053132961 39.0 35.0 41.0 31.0 41.0 58-59 36.40660650455501 39.0 35.0 41.0 31.0 41.0 60-61 36.12704197083859 38.5 35.0 41.0 31.0 41.0 62-63 35.82841133452944 37.5 35.0 40.0 31.0 41.0 64-65 35.57962291660806 37.0 35.0 40.0 31.0 41.0 66-67 35.250458433240624 36.5 35.0 39.5 31.0 41.0 68-69 34.91643009953937 36.0 35.0 39.0 30.0 41.0 70-71 34.5588198097514 35.0 34.5 39.0 30.0 40.5 72-73 34.20440997043427 35.0 34.0 37.5 30.0 40.0 74-75 33.8700567045921 35.0 34.0 37.0 29.5 39.0 76-77 32.423996155168325 34.5 32.0 35.5 27.0 38.5 78-79 33.21524543223603 35.0 34.0 36.0 29.0 37.5 80-81 33.16403327800984 35.0 34.0 36.0 29.0 37.0 82-83 32.98841210454471 35.0 34.0 35.0 29.0 37.0 84-85 32.8058193725353 35.0 34.0 35.0 29.0 36.0 86-87 32.6439084640174 35.0 34.0 35.0 29.0 36.0 88-89 32.506041520749164 35.0 34.0 35.0 29.0 36.0 90-91 32.38556565846318 35.0 34.0 35.0 29.0 35.5 92-93 32.25454863278186 35.0 34.0 35.0 29.0 35.0 94-95 32.14936240113171 35.0 34.0 35.0 29.0 35.0 96-97 32.038098651114424 35.0 34.0 35.0 28.5 35.0 98-99 31.915827854623025 35.0 33.5 35.0 27.0 35.0 100 31.590647604298926 35.0 33.0 35.0 26.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.409497387398865 1101 2 0.3691503180007203 1101 3 0.34774599224169833 1101 4 0.2607446784401546 1101 5 0.27425843569098873 1101 6 0.30122145394727795 1101 7 0.2718402482733566 1101 8 0.29331919973426324 1101 9 0.3462676487048526 1101 10-11 0.3631783874383032 1101 12-13 0.35360961181885386 1101 14-15 0.4722833670060993 1101 16-17 0.44063643623065474 1101 18-19 0.4696020669561065 1101 20-21 0.4808829279161486 1101 22-23 0.4631411526540461 1101 24-25 0.49396783770131236 1101 26-27 0.5550487496521441 1101 28-29 0.5372635171073057 1101 30-31 0.5367740848461935 1101 32-33 0.5718538796771782 1101 34-35 0.503859990316208 1101 36-37 0.5811808111084744 1101 38-39 0.5723637885378992 1101 40-41 0.5978352209531081 1101 42-43 0.6337558241532264 1101 44-45 0.6804977115104194 1101 46-47 0.7274541486793922 1101 48-49 0.6885898116598312 1101 50-51 0.7637075325123277 1101 52-53 0.6590041681433121 1101 54-55 0.7109937134134228 1101 56-57 0.6963257498357223 1101 58-59 0.7733138661602581 1101 60-61 0.7416910417445592 1101 62-63 0.8163127584850045 1101 64-65 0.7013209421826758 1101 66-67 0.7129353657396322 1101 68-69 0.7108540763728328 1101 70-71 0.7346933178130399 1101 72-73 0.7491575623573326 1101 74-75 0.77112021815676 1101 76-77 0.7568570507349222 1101 78-79 0.7399217060652035 1101 80-81 0.7614880675370657 1101 82-83 0.7867481781089367 1101 84-85 0.8071563326917257 1101 86-87 0.7685815296193255 1101 88-89 0.762804912238245 1101 90-91 0.7209275323029019 1101 92-93 0.8136263139275997 1101 94-95 0.7722682190888648 1101 96-97 0.8703375868505603 1101 98-99 0.8719866496513582 1101 100 0.924083665051942 1102 1 0.4014397669683305 1102 2 0.33819800678942613 1102 3 0.3857168700626161 1102 4 0.2514629453501982 1102 5 0.2689238783164427 1102 6 0.2776994301356268 1102 7 0.29170977119495234 1102 8 0.3329176951637507 1102 9 0.38301734299221835 1102 10-11 0.38597189239936114 1102 12-13 0.404392783871657 1102 14-15 0.47795385701552107 1102 16-17 0.5124672351556896 1102 18-19 0.5043445360663341 1102 20-21 0.5062532777799902 1102 22-23 0.5188035101717219 1102 24-25 0.4849849230781942 1102 26-27 0.5139355270302488 1102 28-29 0.5831954970892284 1102 30-31 0.5884787884746174 1102 32-33 0.5890400494402641 1102 34-35 0.5670882918637616 1102 36-37 0.6357219563966865 1102 38-39 0.6379005865746734 1102 40-41 0.6946570717558131 1102 42-43 0.6490302630053364 1102 44-45 0.6801800204236557 1102 46-47 0.7010077141757165 1102 48-49 0.7322272978030426 1102 50-51 0.782201985629051 1102 52-53 0.7920078914671294 1102 54-55 0.8817439030326497 1102 56-57 0.8783043505470545 1102 58-59 0.8520978145504898 1102 60-61 0.8600655673988129 1102 62-63 0.7711994929978445 1102 64-65 0.8476440319103702 1102 66-67 0.8556131345077702 1102 68-69 0.8464251666149138 1102 70-71 0.8292366658991597 1102 72-73 0.812553193196969 1102 74-75 0.8288193897856786 1102 76-77 0.8546765665718468 1102 78-79 0.8154617666242814 1102 80-81 0.7749521994382249 1102 82-83 0.7706568584424787 1102 84-85 0.7643182835848066 1102 86-87 0.7693133109922314 1102 88-89 0.7859583067498264 1102 90-91 0.7727406016815124 1102 92-93 0.8358457221251072 1102 94-95 0.848307505096578 1102 96-97 0.8243499534771033 1102 98-99 0.856866787677486 1102 100 0.9140579578049746 1103 1 0.3625757317930365 1103 2 0.30440062408290913 1103 3 0.3159186798507392 1103 4 0.24663361959499497 1103 5 0.2607048047735532 1103 6 0.278814319957732 1103 7 0.2603636109495113 1103 8 0.2784418304785774 1103 9 0.33119302421395247 1103 10-11 0.3451756344324579 1103 12-13 0.35460754924032756 1103 14-15 0.45298084411096085 1103 16-17 0.43984676933158795 1103 18-19 0.4350601322337937 1103 20-21 0.4462469552792143 1103 22-23 0.48605532983181376 1103 24-25 0.5041228445505084 1103 26-27 0.5072811947861524 1103 28-29 0.5227249699257683 1103 30-31 0.5719091111883614 1103 32-33 0.5511829113368876 1103 34-35 0.5614039865291218 1103 36-37 0.5930543047509502 1103 38-39 0.5506041766459049 1103 40-41 0.5670085318098117 1103 42-43 0.5851700102384143 1103 44-45 0.5532926652033794 1103 46-47 0.540161186781539 1103 48-49 0.54301803190085 1103 50-51 0.5756006292862494 1103 52-53 0.6156020667800917 1103 54-55 0.6704263826065286 1103 56-57 0.6190373613219222 1103 58-59 0.6162603494074332 1103 60-61 0.6527104358575215 1103 62-63 0.644472884586925 1103 64-65 0.6465257700878482 1103 66-67 0.6246930220781834 1103 68-69 0.6003208032020098 1103 70-71 0.5306232235969759 1103 72-73 0.5816166422653524 1103 74-75 0.5453893776509133 1103 76-77 0.491014551934704 1103 78-79 0.5818645742583186 1103 80-81 0.5600168331435853 1103 82-83 0.5924491875852169 1103 84-85 0.6123905863727046 1103 86-87 0.6328072358454193 1103 88-89 0.6566886332348929 1103 90-91 0.6872006268384112 1103 92-93 0.6713256664870997 1103 94-95 0.6830374233921148 1103 96-97 0.7346581809362718 1103 98-99 0.7718395343714768 1103 100 0.8353883007063594 1104 1 0.30428440735855844 1104 2 0.18986325220912192 1104 3 0.22612180715572094 1104 4 0.17384535302143433 1104 5 0.22812669763154503 1104 6 0.24561322130593055 1104 7 0.22606293238082031 1104 8 0.18782414488195798 1104 9 0.2652517901100069 1104 10-11 0.2759230213906321 1104 12-13 0.2766464872572385 1104 14-15 0.376439884391921 1104 16-17 0.36795784309622803 1104 18-19 0.2996709308342247 1104 20-21 0.3079176694228565 1104 22-23 0.390592635523646 1104 24-25 0.371609963838317 1104 26-27 0.38461124510284606 1104 28-29 0.40253358345646717 1104 30-31 0.4470408534213135 1104 32-33 0.4379383687342866 1104 34-35 0.4613167399134923 1104 36-37 0.45005811405120255 1104 38-39 0.48289494444705383 1104 40-41 0.456076275151176 1104 42-43 0.4441602376697702 1104 44-45 0.43370227159656594 1104 46-47 0.4294946467612917 1104 48-49 0.48692584771966807 1104 50-51 0.4710086627810526 1104 52-53 0.486995830583588 1104 54-55 0.5082586008522227 1104 56-57 0.4940744418188814 1104 58-59 0.5128908497888389 1104 60-61 0.5737611969872276 1104 62-63 0.5768306860943895 1104 64-65 0.5882245281501568 1104 66-67 0.5891225996011613 1104 68-69 0.5448848092013066 1104 70-71 0.5199697104570546 1104 72-73 0.5458227675737959 1104 74-75 0.5956046628553935 1104 76-77 0.5025602389643069 1104 78-79 0.6191995192985047 1104 80-81 0.5789625537967495 1104 82-83 0.6054285657184124 1104 84-85 0.6152901490666096 1104 86-87 0.6326510229320377 1104 88-89 0.6116048678293282 1104 90-91 0.5502863327459266 1104 92-93 0.5683147830014477 1104 94-95 0.6451667275836144 1104 96-97 0.6887993034237638 1104 98-99 0.6290992700799087 1104 100 0.6335809537181625 1105 1 0.21733084399335922 1105 2 0.17170633001751412 1105 3 0.1847090247302603 1105 4 0.14028436135279776 1105 5 0.1796039999127217 1105 6 0.1840474735079738 1105 7 0.15199748893159892 1105 8 0.13835729551548326 1105 9 0.21193625952663098 1105 10-11 0.18505066628066658 1105 12-13 0.2251461730101454 1105 14-15 0.2419926984198142 1105 16-17 0.26842460979751337 1105 18-19 0.24157437793432024 1105 20-21 0.2917486571409782 1105 22-23 0.3096915237625879 1105 24-25 0.2900782034231 1105 26-27 0.3215912597693844 1105 28-29 0.29553361787578325 1105 30-31 0.2573941857358619 1105 32-33 0.27456353397163724 1105 34-35 0.3488945906765153 1105 36-37 0.24509672173762453 1105 38-39 0.2976831072565318 1105 40-41 0.3003300420713799 1105 42-43 0.2622673375188427 1105 44-45 0.1949612324064347 1105 46-47 0.22504474202698788 1105 48-49 0.2177219615458057 1105 50-51 0.1915274713426598 1105 52-53 0.21744962887053276 1105 54-55 0.3029213302773073 1105 56-57 0.25892106640838364 1105 58-59 0.19039571171125402 1105 60-61 0.2756689266871959 1105 62-63 0.23495244002150883 1105 64-65 0.23835716017170938 1105 66-67 0.22137748828981074 1105 68-69 0.23089034982788093 1105 70-71 0.22050880962667208 1105 72-73 0.2089093730504601 1105 74-75 0.21892669330681258 1105 76-77 0.18925427829926633 1105 78-79 0.2500852583118416 1105 80-81 0.2569005729752476 1105 82-83 0.23650917444771835 1105 84-85 0.24109505089223404 1105 86-87 0.19558761627887833 1105 88-89 0.2673974345974699 1105 90-91 0.2576723483683523 1105 92-93 0.30441577967845745 1105 94-95 0.3098131560297688 1105 96-97 0.33636116417569184 1105 98-99 0.35902713125265606 1105 100 0.42555245293631927 1106 1 0.1573911410046236 1106 2 0.0632349194230315 1106 3 0.16180891437489464 1106 4 0.13494327937553408 1106 5 0.18533981042344294 1106 6 0.12520676463741154 1106 7 0.11465306277670351 1106 8 0.12227072791485938 1106 9 0.15452105612832412 1106 10-11 0.1624638845974502 1106 12-13 0.17940637825778083 1106 14-15 0.24087597007478223 1106 16-17 0.19458479259780148 1106 18-19 0.15450373160582132 1106 20-21 0.1883800883439548 1106 22-23 0.2282324603655823 1106 24-25 0.23569844052931188 1106 26-27 0.26624760924893565 1106 28-29 0.2767142035161143 1106 30-31 0.19531943221801384 1106 32-33 0.1914553234629608 1106 34-35 0.19985581216975135 1106 36-37 0.1865254659260671 1106 38-39 0.1572875125696669 1106 40-41 0.24871487476227117 1106 42-43 0.2192943621288137 1106 44-45 0.2906446735932562 1106 46-47 0.24700666618596756 1106 48-49 0.270017836795013 1106 50-51 0.2314303056976712 1106 52-53 0.255994550995041 1106 54-55 0.2755887510979065 1106 56-57 0.3079800252974394 1106 58-59 0.3339767879009301 1106 60-61 0.3168583764012567 1106 62-63 0.2302763395567453 1106 64-65 0.31465573953454395 1106 66-67 0.3195003015784508 1106 68-69 0.2650146979390655 1106 70-71 0.28130572290808686 1106 72-73 0.3071722603339353 1106 74-75 0.30128073921989085 1106 76-77 0.22414014227103962 1106 78-79 0.2667801890858783 1106 80-81 0.28390803266139386 1106 82-83 0.2824605800423825 1106 84-85 0.2601596567366826 1106 86-87 0.23418705295280517 1106 88-89 0.2818046821032354 1106 90-91 0.3019010782030236 1106 92-93 0.3195648970091085 1106 94-95 0.3294561760420578 1106 96-97 0.4014933160026217 1106 98-99 0.43459439973488045 1106 100 0.4705253003711469 1107 1 0.16009702668015535 1107 2 0.08993362667990823 1107 3 0.12031910184276029 1107 4 0.14224223230057476 1107 5 0.17490056902072126 1107 6 0.15267145255441505 1107 7 0.0936114204722216 1107 8 0.15417459775402875 1107 9 0.08078556284689142 1107 10-11 0.15776635931053562 1107 12-13 0.17055358955255429 1107 14-15 0.20396254177083506 1107 16-17 0.2227280532302629 1107 18-19 0.22474751422132755 1107 20-21 0.26456562601480726 1107 22-23 0.21784327551807792 1107 24-25 0.23688349688713828 1107 26-27 0.26333080733393643 1107 28-29 0.2852823171008865 1107 30-31 0.3055958811930992 1107 32-33 0.304732970813852 1107 34-35 0.3103575605908375 1107 36-37 0.33732363275643706 1107 38-39 0.3451753544227145 1107 40-41 0.3427602422423348 1107 42-43 0.3399369753297137 1107 44-45 0.35255740333781915 1107 46-47 0.3407193545937943 1107 48-49 0.38201706429696713 1107 50-51 0.40087925883620557 1107 52-53 0.3726607169962719 1107 54-55 0.43557593655983595 1107 56-57 0.40690083533266375 1107 58-59 0.3836435357478152 1107 60-61 0.3179516649049319 1107 62-63 0.29883973664838237 1107 64-65 0.2952527864375085 1107 66-67 0.2788507305013965 1107 68-69 0.2817160278837534 1107 70-71 0.3248307591638877 1107 72-73 0.3023024736750912 1107 74-75 0.28968111764804405 1107 76-77 0.24696485270974478 1107 78-79 0.3756802625825628 1107 80-81 0.342220540934548 1107 82-83 0.3336798601913671 1107 84-85 0.346743818234323 1107 86-87 0.3639631895800832 1107 88-89 0.3559270012364806 1107 90-91 0.398604739425771 1107 92-93 0.3937512363939035 1107 94-95 0.41227664942784514 1107 96-97 0.43910256188735985 1107 98-99 0.44749046511135404 1107 100 0.5368926215461265 1108 1 0.10969974270000904 1108 2 0.04214309657683657 1108 3 0.07908376800124728 1108 4 0.0701108736708278 1108 5 0.08206752204171863 1108 6 0.07470754407127345 1108 7 0.0631330884987662 1108 8 0.07838197466885077 1108 9 0.0789040956076974 1108 10-11 0.09539821725431352 1108 12-13 0.0839989523984741 1108 14-15 0.06906959037944205 1108 16-17 0.1509955245975405 1108 18-19 0.11240470142787728 1108 20-21 0.13590706885376136 1108 22-23 0.10568047838810202 1108 24-25 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sequence quality scores pass #Quality Count 2 23.0 3 43.0 4 80.0 5 133.0 6 307.0 7 709.0 8 1450.0 9 2525.0 10 4243.0 11 7285.0 12 11214.0 13 15460.0 14 18184.0 15 23880.0 16 32616.0 17 42994.0 18 43085.0 19 28214.0 20 20225.0 21 18069.0 22 19626.0 23 22523.0 24 26193.0 25 31912.0 26 40863.0 27 53168.0 28 69138.0 29 89543.0 30 114884.0 31 147480.0 32 191371.0 33 254069.0 34 364388.0 35 593671.0 36 1153096.0 37 2248812.0 38 2227518.0 39 470080.0 40 370.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.974445028776636 19.197684614141295 13.527380360367147 24.30048999671492 2 16.07528829829639 22.125181203974428 36.47540289482667 25.324127602902507 3 19.08640181875975 26.11786885195083 29.78755621327314 25.00817311601628 4 14.120656214055963 18.189766988770533 35.32552325934159 32.364053537831914 5 14.444139563956801 36.766083664185615 33.390687153999714 15.39908961785787 6 31.826793289281145 35.13527237323474 17.653100729917263 15.384833607566842 7 26.54124635673115 31.922449211175376 22.385965029386927 19.150339402706546 8 26.930449741365457 32.12677741218607 21.20036798624557 19.742404860202893 9 27.282928403837015 14.82838433631597 20.031553938496995 37.85713332135002 10-11 25.412751128263896 25.410128783358317 26.76515399482063 22.41196609355716 12-13 26.774666051527575 23.432991071324803 26.735932790670358 23.056410086477264 14-15 24.886204737425484 23.889632750054265 24.76940866752354 26.45475384499671 16-17 23.70737447430674 26.602224617308657 26.22730498660333 23.463095921781278 18-19 24.502410410034326 25.864699734571207 27.412776102921722 22.220113752472752 20-21 25.05306381431393 25.3897947138291 26.590536167877442 22.96660530397953 22-23 24.119644206482686 25.758142515522962 25.586990284804322 24.53522299319003 24-25 24.121044557470807 26.28640461344627 25.359930817377663 24.23262001170526 26-27 24.459526435853235 25.59667542454158 25.637679539018393 24.30611860058679 28-29 24.03084123691088 25.125145606856957 27.029353102647885 23.814660053584277 30-31 24.985907585467857 25.1814610695931 26.360938651955802 23.471692692983233 32-33 23.416442393905214 25.767462224075366 26.480140626113762 24.33595475590565 34-35 23.574910080630477 26.01157316476666 26.672019900536654 23.741496854066206 36-37 24.41467625082875 25.914476349003557 25.898056267655313 23.77279113251238 38-39 24.543700699008777 25.0610748348436 26.039047007879933 24.356177458267688 40-41 24.03267038537988 25.655670391995393 26.143442330572675 24.168216892052044 42-43 25.210220613412034 25.220030819304174 25.88898434866878 23.680764218615014 44-45 24.87363704149194 25.115012358714235 26.069702397345612 23.941648202448214 46-47 24.121945511001762 25.278941085647304 26.911710987062463 23.68740241628847 48-49 24.640259192415993 25.739956752790384 26.37933173871019 23.24045231608344 50-51 23.72920873672011 26.147389746511053 25.738129405845388 24.385272110923452 52-53 23.558128382234035 26.150002783449644 25.061912383680713 25.22995645063561 54-55 24.10996284998577 25.751257333514143 25.047603578203535 25.091176238296555 56-57 24.363457002318732 24.95205271375798 25.28959135534811 25.394898928575177 58-59 23.99664390940439 24.983082730411244 26.298519298504573 24.72175406167979 60-61 24.192417069974265 25.255975931263798 25.83382494892285 24.71778204983909 62-63 22.989861070191825 25.616713323096274 26.132805932001396 25.260619674710505 64-65 22.972866654913044 25.577527836476357 25.942045104109564 25.50756040450104 66-67 23.971415948407003 25.53394099851915 25.322550086367574 25.172092966706273 68-69 24.10075942903181 24.884385866855382 25.383782183294993 25.631072520817817 70-71 23.326277628668173 25.261957637402976 25.567163152581664 25.84460158134719 72-73 24.100036456799586 24.95055207585923 25.548105065909805 25.401306401431377 74-75 24.143758624339736 24.752254367837686 25.632676079574225 25.471310928248354 76-77 23.327264532907588 25.380121503948395 26.118747837289558 25.17386612585446 78-79 23.429282262499388 25.287294598747707 26.008447742888997 25.27497539586391 80-81 22.956503434554186 25.29668235463518 25.84594402203531 25.900870188775322 82-83 22.884894069406553 25.558949082689786 25.15733214929069 26.398824698612973 84-85 23.047690361748156 25.297101124073635 25.052698680835906 26.602509833342303 86-87 23.127191156844994 24.678689928390625 25.48251341811647 26.711605496647916 88-89 22.999913224202437 24.320019844003816 26.058539572862827 26.621527358930923 90-91 23.328293635581694 24.61553232939884 25.660643822579754 26.39553021243971 92-93 22.505094981662836 24.876079973704858 25.855538235382642 26.76328680924967 94-95 22.039859571580678 24.99997765017518 26.202314786256704 26.75784799198744 96-97 22.785030717509112 24.746772234382693 25.6952450961081 26.772951952000096 98-99 22.71593326089309 24.43048073269218 25.692602513348916 27.16098349306581 100 22.61261830405035 24.755560588361504 25.816589982597087 26.81523112499106 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 785.0 1 509.0 2 267.5 3 344.5 4 334.0 5 394.0 6 665.0 7 1298.0 8 1967.5 9 2039.5 10 1575.5 11 1373.0 12 1552.0 13 1748.5 14 2047.5 15 2289.5 16 2592.0 17 2972.5 18 3444.5 19 4036.5 20 4734.0 21 5549.0 22 6668.5 23 8122.0 24 9833.0 25 12373.5 26 16091.5 27 21378.0 28 27886.5 29 37278.5 30 48337.0 31 58212.5 32 69558.0 33 81893.0 34 94956.5 35 107170.0 36 118973.0 37 131848.5 38 142711.5 39 154456.5 40 164834.0 41 173775.5 42 184760.5 43 204661.5 44 231110.5 45 255632.5 46 286398.5 47 323729.5 48 398851.5 49 476176.0 50 553269.0 51 537112.0 52 425301.0 53 375251.5 54 350714.0 55 322074.5 56 296873.0 57 270284.0 58 251405.5 59 228301.0 60 192543.5 61 157160.0 62 123906.5 63 95791.5 64 72162.0 65 55429.5 66 43354.0 67 32617.5 68 24340.0 69 19136.5 70 15520.5 71 12537.5 72 10080.5 73 9305.5 74 7001.5 75 5466.0 76 4229.5 77 2792.5 78 2003.5 79 1103.0 80 666.5 81 558.0 82 452.5 83 326.5 84 223.0 85 139.0 86 86.5 87 46.0 88 29.5 89 17.0 90 8.5 91 7.5 92 5.0 93 1.5 94 3.0 95 2.5 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.06253096152736701 3 0.026104232890761296 4 0.0063770614596152025 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 7.151844627605835E-5 12-13 1.6091650412113125E-4 14-15 4.1719093661034035E-4 16-17 5.483080881164474E-4 18-19 0.0 20-21 0.0030097346141174553 22-23 0.005018210980370094 24-25 6.43666016484525E-4 26-27 5.006291239324084E-4 28-29 0.004958612275140045 30-31 0.0021336336472357405 32-33 0.0012634925508770308 34-35 0.0014601682781361911 36-37 0.00388583558099917 38-39 0.0041778692366264076 40-41 8.64181225835705E-4 42-43 0.002640222641691154 44-45 2.324349503971896E-4 46-47 0.002056155330436677 48-49 1.1919741046009724E-5 50-51 0.001942917790499585 52-53 0.006830011619363572 54-55 0.013785180519710246 56-57 0.025126814124988496 58-59 0.02746904324052941 60-61 0.025412887910092732 62-63 0.0076226743989232185 64-65 0.00644857990589126 66-67 1.251572809831021E-4 68-69 0.0013707702202911181 70-71 1.7879611569014586E-4 72-73 7.449838153756077E-4 74-75 0.004720217454219851 76-77 0.001037017471002846 78-79 0.0012217734572159966 80-81 0.0 82-83 0.0012694524214000356 84-85 2.9799352615024308E-5 86-87 4.767896418403889E-4 88-89 9.53579283680778E-5 90-91 6.198265343925056E-4 92-93 7.33064074329598E-4 94-95 0.0013886498318601327 96-97 1.251572809831021E-4 98-99 0.0 100 5.244686060244278E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8389444.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.91782829590532 #Duplication Level Percentage of deduplicated Percentage of total 1 76.47074099982648 32.819581318912 2 13.24999924111469 11.373223847020721 3 4.137449580490831 5.32711052035413 4 1.8611845968668568 3.1951200382126212 5 1.0186083508195916 2.1858229150625257 6 0.6347905074143845 1.6346298000648698 7 0.44074990661695 1.3241220169535797 8 0.2985256900615238 1.0249659446381703 9 0.23309509039456394 0.9003541559555184 >10 1.402140752326446 11.334644828887441 >50 0.12989538014753435 3.8810617580909237 >100 0.10077134084621275 8.865781574363469 >500 0.013815521054226298 4.08627803861473 >1k 0.007200423197165358 5.598003530394467 >5k 5.860809533839404E-4 1.8056491666754722 >10k+ 4.4653786924490695E-4 4.643650545799318 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 59055 0.7039203074721043 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 54617 0.6510204967099131 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 30320 0.36140654851501486 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 25909 0.30882857076106596 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 25856 0.3081968244856274 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 25469 0.30358388470082165 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 23332 0.2781113980854989 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 20628 0.24588041829708857 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 19833 0.23640422416551085 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 19775 0.23571287918484227 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 18296 0.2180835821777939 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 16531 0.19704523923158673 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14575 0.17373022574559172 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 11573 0.13794716312547053 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 11529 0.1374226945194461 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10391 0.12385802920908703 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 9911 0.11813655350700236 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 9688 0.1154784512537422 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 9202 0.10968545710538147 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 9016 0.10746838527082365 No Hit CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC 8531 0.10168731086350896 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0017998808979474683 0.0 0.0 1.1919741046009724E-5 1.1919741046009724E-5 2 0.0029560957794104114 0.0 0.0 1.1919741046009724E-5 1.1919741046009724E-5 3 0.003075293189870509 1.1919741046009724E-5 0.0 1.1919741046009724E-5 1.1919741046009724E-5 4 0.0035997617958949367 1.1919741046009724E-5 0.0 4.76789641840389E-5 1.1919741046009724E-5 5 0.014029535211153445 2.383948209201945E-5 0.0 1.1919741046009723E-4 2.383948209201945E-5 6 0.02927488400899988 2.383948209201945E-5 0.0 3.2183300824226255E-4 2.383948209201945E-5 7 0.03264817072502063 2.383948209201945E-5 0.0 4.052711955643306E-4 3.575922313802917E-5 8 0.03729686973296443 2.383948209201945E-5 0.0 4.767896418403889E-4 3.575922313802917E-5 9 0.040443681369110994 2.383948209201945E-5 0.0 5.60227829162457E-4 3.575922313802917E-5 10-11 0.07198927604737573 2.383948209201945E-5 0.0 9.595391542037827E-4 6.555857575305348E-5 12-13 0.12510960201891808 4.76789641840389E-5 0.0 0.0017820012863784538 7.151844627605835E-5 14-15 0.18774188134517616 1.0727766941408751E-4 0.0 0.0026819417353521877 8.343818732206806E-5 16-17 0.2160035873652652 1.1919741046009723E-4 0.0 0.003140851765623562 9.53579283680778E-5 18-19 0.2298364468491595 1.370770220291118E-4 0.0 0.003367326845497747 1.0727766941408751E-4 20-21 0.27735449452907723 1.6687637464413613E-4 0.0 0.004553341079575715 1.0727766941408751E-4 22-23 0.2914734277980758 1.7283624516714098E-4 0.0 0.005489040751687478 1.0727766941408751E-4 24-25 0.3556016346256081 2.026355977821653E-4 0.0 0.010477452379442547 1.0727766941408751E-4 26-27 0.4950387653818299 2.026355977821653E-4 0.0 0.02723064841960921 1.0727766941408751E-4 28-29 0.5276452170131893 2.026355977821653E-4 0.0 0.04671346515931211 1.0727766941408751E-4 30-31 0.5383193451198911 2.026355977821653E-4 0.0 0.09737832447537645 1.0727766941408751E-4 32-33 0.5482663690227862 2.026355977821653E-4 0.0 0.1933322398957547 1.1919741046009723E-4 34-35 0.5559069230332785 2.0859546830517015E-4 0.0 0.3265472658259594 1.251572809831021E-4 36-37 0.5679160621371333 2.1455533882817503E-4 0.0 0.5249453956662682 1.430368925521167E-4 38-39 0.5793649734118256 2.2647507987418476E-4 0.0 0.8080928843437062 1.4899676307512154E-4 40-41 0.5900987002237574 2.503145619662042E-4 0.0 1.1715615480596808 1.6687637464413613E-4 42-43 0.6127164088585608 2.503145619662042E-4 0.0 1.4658539946151379 1.8475598621315074E-4 44-45 0.638570327187356 2.503145619662042E-4 0.0 1.7959593031433312 1.907158567361556E-4 46-47 0.6486365485007112 2.503145619662042E-4 0.0 2.178136000431018 2.026355977821653E-4 48-49 0.6566108552604917 2.503145619662042E-4 0.0 2.5401266162572873 2.1455533882817503E-4 50-51 0.6690193056893878 2.5627443248920907E-4 0.0 2.919543893492823 2.324349503971896E-4 52-53 0.6735190079342565 2.622343030122139E-4 0.0 3.3422119511138044 2.4435469144319937E-4 54-55 0.686404248004993 2.681941735352188E-4 0.0 3.75344301720114 2.5627443248920907E-4 56-57 0.7107324394798988 2.741540440582236E-4 0.0 4.199986316137279 2.622343030122139E-4 58-59 0.7188497831322314 2.741540440582236E-4 0.0 4.664391347030865 2.622343030122139E-4 60-61 0.7248275332668053 2.801139145812285E-4 0.0 5.10706073012705 2.681941735352188E-4 62-63 0.7295179513684101 2.9203365562723824E-4 0.0 5.555934338437684 2.8607378510423333E-4 64-65 0.7333501481147022 2.979935261502431E-4 0.0 6.0415386287815975 3.158731377192577E-4 66-67 0.7382134024614742 2.979935261502431E-4 0.0 6.5678190354450185 3.2183300824226255E-4 68-69 0.743994476868789 3.277928787652674E-4 0.0 7.156910517550388 3.2183300824226255E-4 70-71 0.7510688431795957 3.516323608572868E-4 0.0 7.818903135893153 3.2183300824226255E-4 72-73 0.7685610631646149 3.695119724263015E-4 0.0 8.411940052284752 3.2183300824226255E-4 74-75 0.785683771177208 3.814317134723112E-4 0.0 8.999178014657467 3.277928787652674E-4 76-77 0.7911370527057574 3.933514545183209E-4 0.0 9.640716357365278 3.3375274928827226E-4 78-79 0.7951301659561707 4.052711955643306E-4 0.0 10.299401247567776 3.575922313802917E-4 80-81 0.8027945594487549 4.1123106608733544E-4 0.0 11.010968068920896 3.6355210190329657E-4 82-83 0.8058996519912405 4.1719093661034035E-4 0.0 11.78339708805494 3.8739158399531603E-4 84-85 0.8177061554973131 4.231508071333452E-4 0.0 12.539043111796204 3.933514545183209E-4 86-87 0.8376836414904254 4.2911067765635005E-4 0.0 13.303175991162227 3.9931132504132574E-4 88 0.842272741793139 4.529501597483695E-4 0.0 13.911207941789707 4.1719093661034035E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTAAC 775 0.0 21.243717 1 TCCGATC 1975 0.0 20.708544 3 AGTACTT 19230 0.0 18.254414 12-13 GAGTACT 17665 0.0 17.424255 12-13 GTATAAG 2455 0.0 16.286646 1 CGATCTC 2290 0.0 16.210829 5 TACTTTT 22555 0.0 15.84584 14-15 GTATATA 1955 0.0 15.639785 1 GTATAAA 4525 0.0 15.593258 1 GTACTTT 20370 0.0 15.296125 14-15 CTAACGC 1165 0.0 14.93046 3 ACTGCGC 2185 0.0 14.839402 8 TCTCGCG 1020 0.0 14.742413 8 CCGATCT 2915 0.0 14.509112 4 TCTACAC 4525 0.0 14.440879 3 TCTAACG 1215 0.0 13.937677 2 ATATACG 2775 0.0 13.885593 6 ACTTTTT 23070 0.0 13.6598625 16-17 GTATTAG 3330 0.0 13.278463 1 CATGGGG 15790 0.0 13.243866 4 >>END_MODULE