##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765542_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6371218 Sequences flagged as poor quality 0 Sequence length 99 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.436669566164586 33.0 33.0 33.0 22.0 33.0 2 30.187287265951344 33.0 33.0 33.0 22.0 33.0 3 30.289790586352563 33.0 33.0 33.0 22.0 33.0 4 33.47292856719076 37.0 33.0 37.0 22.0 37.0 5 33.931600362756384 37.0 33.0 37.0 27.0 37.0 6 33.64310780136545 37.0 33.0 37.0 27.0 37.0 7 33.59117785641615 37.0 33.0 37.0 27.0 37.0 8 33.37054908496303 37.0 33.0 37.0 22.0 37.0 9 32.98887010301641 37.0 33.0 37.0 15.0 37.0 10-11 33.23917679476672 37.0 33.0 37.0 22.0 37.0 12-13 33.157124195091114 37.0 33.0 37.0 18.5 37.0 14-15 34.42884876957593 37.0 33.0 40.0 18.5 40.0 16-17 34.6296217614905 37.0 33.0 40.0 22.0 40.0 18-19 34.73804074825253 37.0 33.0 40.0 22.0 40.0 20-21 34.84718392935228 37.0 33.0 40.0 24.5 40.0 22-23 35.032448662092555 37.0 33.0 40.0 27.0 40.0 24-25 35.24501311994033 37.0 33.0 40.0 27.0 40.0 26-27 35.26685392023943 37.0 33.0 40.0 27.0 40.0 28-29 35.32457906792705 37.0 33.0 40.0 27.0 40.0 30-31 35.22103395300553 37.0 33.0 40.0 27.0 40.0 32-33 35.059648955662794 37.0 33.0 40.0 27.0 40.0 34-35 34.89521289335885 37.0 33.0 40.0 27.0 40.0 36-37 34.67556391886136 37.0 33.0 40.0 24.5 40.0 38-39 34.31230974987828 37.0 33.0 40.0 22.0 40.0 40-41 34.31492675340884 37.0 33.0 40.0 22.0 40.0 42-43 34.19976494290417 37.0 33.0 40.0 22.0 40.0 44-45 34.05502268169132 37.0 33.0 40.0 22.0 40.0 46-47 33.95143134326906 37.0 33.0 40.0 22.0 40.0 48-49 33.79733498367188 37.0 33.0 40.0 22.0 40.0 50-51 33.60795918457036 37.0 33.0 40.0 22.0 40.0 52-53 33.44113762862925 37.0 33.0 40.0 22.0 40.0 54-55 33.24419632164525 37.0 33.0 37.0 22.0 40.0 56-57 32.99439149625707 37.0 33.0 37.0 22.0 40.0 58-59 32.71055236220138 37.0 33.0 37.0 22.0 40.0 60-61 32.46066223130334 37.0 33.0 37.0 22.0 40.0 62-63 32.14605825762044 37.0 27.0 37.0 22.0 40.0 64-65 31.922740439897048 37.0 27.0 37.0 22.0 40.0 66-67 31.71769707142339 33.0 27.0 37.0 18.5 38.5 68-69 31.461105867041436 33.0 27.0 37.0 15.0 37.0 70-71 31.17899450309187 33.0 27.0 37.0 15.0 37.0 72-73 30.80929682519104 33.0 27.0 37.0 15.0 37.0 74-75 30.53289355347753 33.0 27.0 37.0 15.0 37.0 76-77 30.30443998306132 33.0 27.0 37.0 15.0 37.0 78-79 30.03595654708409 33.0 27.0 37.0 15.0 37.0 80-81 29.86083924612217 33.0 27.0 37.0 15.0 37.0 82-83 29.680575754902755 33.0 27.0 37.0 15.0 37.0 84-85 29.50749566252481 33.0 27.0 37.0 15.0 37.0 86-87 29.196388586923256 33.0 27.0 37.0 15.0 37.0 88-89 29.016531218991407 33.0 27.0 37.0 15.0 37.0 90-91 28.83306520040595 33.0 27.0 37.0 15.0 37.0 92-93 28.48466549096264 33.0 27.0 37.0 6.0 37.0 94-95 28.35036252094968 33.0 27.0 37.0 6.0 37.0 96-97 28.141850349493613 33.0 22.0 37.0 6.0 37.0 98-99 27.085269959370407 33.0 22.0 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5219887361037792 1101 2 0.6271366380101853 1101 3 0.5712511504915199 1101 4 0.6306262589953135 1101 5 0.493671279885433 1101 6 0.66132010909066 1101 7 0.4872442123973286 1101 8 0.5376206216587889 1101 9 0.5025188482320999 1101 10-11 0.40324085057731907 1101 12-13 0.5874290292172475 1101 14-15 0.7331539236114679 1101 16-17 0.6379346397730572 1101 18-19 0.5698332519053722 1101 20-21 0.5486544195152021 1101 22-23 0.5249541169376144 1101 24-25 0.4177229451958411 1101 26-27 0.46673990188301673 1101 28-29 0.5126863639474948 1101 30-31 0.7420333930741094 1101 32-33 0.8137108405365936 1101 34-35 0.8768323540012233 1101 36-37 0.9028234760725766 1101 38-39 0.9245080197219337 1101 40-41 0.6842633976282997 1101 42-43 0.6481383074818865 1101 44-45 0.5667762262488694 1101 46-47 0.5713710544329373 1101 48-49 0.4689244914301014 1101 50-51 0.46633959302271677 1101 52-53 0.5344338859420645 1101 54-55 0.34573646081946663 1101 56-57 0.5133753887393055 1101 58-59 0.48446302449119116 1101 60-61 0.2866533291696598 1101 62-63 0.49892663332133935 1101 64-65 0.5947419441134336 1101 66-67 0.5270797858496081 1101 68-69 0.6310057783597003 1101 70-71 0.5624046296206515 1101 72-73 0.3896081657104382 1101 74-75 0.4225220453183063 1101 76-77 0.5454585315136704 1101 78-79 0.545984235832826 1101 80-81 0.5139838211031318 1101 82-83 0.5837768899916327 1101 84-85 0.5155859946215919 1101 86-87 0.48164639726638114 1101 88-89 0.3727852130289442 1101 90-91 0.3315076636583001 1101 92-93 0.4357263231704884 1101 94-95 0.498220474484814 1101 96-97 0.434935378104079 1101 98-99 0.36291067133807786 1102 1 0.5287021471664168 1102 2 0.5861995700281284 1102 3 0.5116142228564691 1102 4 0.5606168733439745 1102 5 0.4378844173458276 1102 6 0.5828853284233517 1102 7 0.5612061873765128 1102 8 0.5026082898585074 1102 9 0.39885699959148724 1102 10-11 0.3737408488262588 1102 12-13 0.5165211556888991 1102 14-15 0.6401574077718664 1102 16-17 0.48149333161287444 1102 18-19 0.45648344333938695 1102 20-21 0.42191986884888166 1102 22-23 0.46842755431859473 1102 24-25 0.38321288144871346 1102 26-27 0.48291455510858583 1102 28-29 0.4493036574478424 1102 30-31 0.6511284022189869 1102 32-33 0.7086256145323802 1102 34-35 0.8292789928086179 1102 36-37 0.9260645748066594 1102 38-39 0.9181394240937877 1102 40-41 0.7323315906890429 1102 42-43 0.5643734049149245 1102 44-45 0.40631357798928747 1102 46-47 0.4222847764828259 1102 48-49 0.328383128899695 1102 50-51 0.4050629671468613 1102 52-53 0.4729673253892912 1102 54-55 0.3501412818223102 1102 56-57 0.4332806632652222 1102 58-59 0.35353768638447747 1102 60-61 0.36662749455149424 1102 62-63 0.4857720673157857 1102 64-65 0.5242844308207388 1102 66-67 0.5557362609421688 1102 68-69 0.651249586412618 1102 70-71 0.6117775004479995 1102 72-73 0.49316707424323525 1102 74-75 0.5180530043970393 1102 76-77 0.500230132739059 1102 78-79 0.4865150154284912 1102 80-81 0.44504423116503844 1102 82-83 0.4077240247851712 1102 84-85 0.5151784038885197 1102 86-87 0.48907660242866413 1102 88-89 0.3359727098004406 1102 90-91 0.31612611766992416 1102 92-93 0.3908991128364683 1102 94-95 0.40372766081188516 1102 96-97 0.3092392464457987 1102 98-99 0.32688285934178296 1103 1 0.23848330884050384 1103 2 0.32625339044684054 1103 3 0.22678967866636057 1103 4 0.2964379295699686 1103 5 0.2750300503581897 1103 6 0.4000885994939978 1103 7 0.266007065695689 1103 8 0.2650027601342586 1103 9 0.23196280335367447 1103 10-11 0.26971731770840535 1103 12-13 0.24129307738666483 1103 14-15 0.44537498319727575 1103 16-17 0.19766017250848478 1103 18-19 0.21805681534706878 1103 20-21 0.29427197926864324 1103 22-23 0.16482371239290217 1103 24-25 0.11468499537161136 1103 26-27 0.3269303177230398 1103 28-29 0.28876468449117 1103 30-31 0.4507026742548561 1103 32-33 0.5843621945628783 1103 34-35 0.5779591880717092 1103 36-37 0.7372319513293277 1103 38-39 0.7380198695127334 1103 40-41 0.5415250149577702 1103 42-43 0.42634694657606076 1103 44-45 0.31575674315319446 1103 46-47 0.302066574794182 1103 48-49 0.19206958515719919 1103 50-51 0.1161382551269412 1103 52-53 0.21885321269182612 1103 54-55 0.13887910859167363 1103 56-57 0.21384600589018987 1103 58-59 0.15536159208092215 1103 60-61 0.04059648343720568 1103 62-63 0.19825640936973343 1103 64-65 0.32671364423739035 1103 66-67 0.2815940695930337 1103 68-69 0.35926882989317477 1103 70-71 0.3792528766181782 1103 72-73 0.08777657481495282 1103 74-75 0.18751260310153128 1103 76-77 0.18663942323139793 1103 78-79 0.19544507061500482 1103 80-81 0.11142816755111085 1103 82-83 0.21272167643451567 1103 84-85 0.2610067535969698 1103 86-87 0.1610569570913114 1103 88-89 0.16252139546117306 1103 90-91 0.05953578086260336 1103 92-93 0.09684900129390783 1103 94-95 0.2218025625507991 1103 96-97 0.020633519178367976 1103 98-99 0.06015790705954416 1104 1 0.29348802525807116 1104 2 0.2726611243816883 1104 3 0.2467587742177102 1104 4 0.33602623518373065 1104 5 0.25458877924410217 1104 6 0.4307938900246455 1104 7 0.30138422526803055 1104 8 0.29330408015854914 1104 9 0.2925666581503208 1104 10-11 0.3356078403344469 1104 12-13 0.3081818655318287 1104 14-15 0.3110027956869885 1104 16-17 0.21537209416985803 1104 18-19 0.24251070524388751 1104 20-21 0.1905116177326036 1104 22-23 0.12581177154363843 1104 24-25 0.11452815349918666 1104 26-27 0.3011611786713644 1104 28-29 0.20082477628054818 1104 30-31 0.3833247258144823 1104 32-33 0.4596636856962135 1104 34-35 0.5183974194039607 1104 36-37 0.5674712002120472 1104 38-39 0.4736305740093556 1104 40-41 0.33166563980763897 1104 42-43 0.19691174522303356 1104 44-45 0.15372346297722572 1104 46-47 0.1850433694762259 1104 48-49 0.042339948128699234 1104 50-51 0.005603945530602061 1104 52-53 0.17922152321854412 1104 54-55 -0.048663589812569796 1104 56-57 0.13557744698844942 1104 58-59 0.1537928821897694 1104 60-61 -0.003936223382730475 1104 62-63 0.10488750127159818 1104 64-65 0.14937018062727603 1104 66-67 0.11627569951784267 1104 68-69 0.19842147226624007 1104 70-71 0.25802400890099975 1104 72-73 0.021261790478835252 1104 74-75 0.12371634651715624 1104 76-77 0.027059903091824822 1104 78-79 0.10558957574832206 1104 80-81 -8.703219432497633E-4 1104 82-83 0.14183211029155274 1104 84-85 0.23650472411555157 1104 86-87 0.13990106549039538 1104 88-89 0.08487370132301209 1104 90-91 0.0011587215487161018 1104 92-93 0.005554171669700736 1104 94-95 0.08940730084470161 1104 96-97 -0.023723808146836944 1104 98-99 -0.03969101617149917 1105 1 0.1935257399951027 1105 2 0.12165925714212378 1105 3 0.16972615602405128 1105 4 0.13236127785089735 1105 5 0.08735841731425609 1105 6 0.23799936589178827 1105 7 0.14974966385592836 1105 8 0.18910618142272995 1105 9 0.06211901925418317 1105 10-11 0.14461515237334766 1105 12-13 0.15481300319587632 1105 14-15 0.2819049623397234 1105 16-17 0.13613591246757295 1105 18-19 0.15892590586005895 1105 20-21 0.10451950362224949 1105 22-23 0.08232981070950274 1105 24-25 -0.032526446874094006 1105 26-27 0.08045348542607655 1105 28-29 0.11915464227417516 1105 30-31 0.28132936708490774 1105 32-33 0.3263398205553898 1105 34-35 0.4130671929359764 1105 36-37 0.480025983177093 1105 38-39 0.47559788877363474 1105 40-41 0.27824053362934364 1105 42-43 0.08622073146047171 1105 44-45 0.13975812827110445 1105 46-47 0.08202384017045006 1105 48-49 0.017176542791389693 1105 50-51 0.029471315785684737 1105 52-53 0.06383786239574363 1105 54-55 -0.020866617387767405 1105 56-57 0.15059073676916057 1105 58-59 0.032279661810996174 1105 60-61 3.2829740353435E-4 1105 62-63 0.010491287123123527 1105 64-65 0.1386736685886909 1105 66-67 0.10197641136321778 1105 68-69 0.24305097018401867 1105 70-71 0.2956550149521888 1105 72-73 0.03410166499469014 1105 74-75 0.16572038051657145 1105 76-77 0.12528469495200767 1105 78-79 0.06751052490010068 1105 80-81 0.057398559641967495 1105 82-83 0.12258806469696992 1105 84-85 0.1355661395510417 1105 86-87 0.08811192446037097 1105 88-89 0.07675911988123474 1105 90-91 0.08300837681242967 1105 92-93 0.10880796104938995 1105 94-95 0.08702786462609069 1105 96-97 -0.04136294897375592 1105 98-99 -0.0726915065299032 1106 1 0.18976392610981208 1106 2 0.15998950089071684 1106 3 0.08099132203447468 1106 4 0.05121609925194548 1106 5 0.0573902295075186 1106 6 0.11485941587137205 1106 7 0.07948753671929865 1106 8 0.09611072689151712 1106 9 0.05526392506611444 1106 10-11 0.031205809348797686 1106 12-13 0.018319090149198303 1106 14-15 0.16827626950457386 1106 16-17 0.05039510777248779 1106 18-19 0.21198513965610033 1106 20-21 0.045679866582815976 1106 22-23 0.0275632321785082 1106 24-25 0.060650219470069544 1106 26-27 0.22757369565064778 1106 28-29 0.16152113735530804 1106 30-31 0.2856142096299905 1106 32-33 0.3133154297681173 1106 34-35 0.3333754393330324 1106 36-37 0.44907981953743104 1106 38-39 0.40969335926317996 1106 40-41 0.2539422699042504 1106 42-43 0.12770268068173607 1106 44-45 0.15497248577117517 1106 46-47 0.08531603116733777 1106 48-49 -0.0707561305388964 1106 50-51 0.010795768646879367 1106 52-53 0.1111411297695355 1106 54-55 -0.1199289790759579 1106 56-57 -0.033318452734107495 1106 58-59 -0.06270528214164983 1106 60-61 -0.09726715292982391 1106 62-63 0.03561064166074601 1106 64-65 0.09761452771963164 1106 66-67 0.08947496016499201 1106 68-69 0.11579252509580584 1106 70-71 0.07929709595876488 1106 72-73 -0.04289307357538874 1106 74-75 0.02287060433406296 1106 76-77 0.09103327311307297 1106 78-79 0.06737213515814133 1106 80-81 0.025305107160196627 1106 82-83 0.016853565372382917 1106 84-85 0.07669704629197227 1106 86-87 0.13404344471221563 1106 88-89 -0.009868001609721233 1106 90-91 -0.10088826943401585 1106 92-93 -0.1490873406060338 1106 94-95 -0.03664674040619431 1106 96-97 -0.06973594783309522 1106 98-99 -0.10295239652626265 1107 1 0.055486104003477976 1107 2 0.1265346276138608 1107 3 -0.054415466309446714 1107 4 -0.012366161418356114 1107 5 -0.03366497867764906 1107 6 0.07943970388468102 1107 7 0.053617817000066736 1107 8 0.003022754832556984 1107 9 -0.1354056335352496 1107 10-11 -0.02494904642979634 1107 12-13 0.06600585709836793 1107 14-15 0.13585693091746975 1107 16-17 -0.05418294927428491 1107 18-19 0.19648231564316632 1107 20-21 0.07738165312791523 1107 22-23 -0.006197962664217016 1107 24-25 0.07199457410609256 1107 26-27 0.0429200764264408 1107 28-29 0.08181294263489747 1107 30-31 0.2540934377780317 1107 32-33 0.30060176302507813 1107 34-35 0.28725026731037673 1107 36-37 0.41252248098233224 1107 38-39 0.3279459143516661 1107 40-41 0.21810321316564796 1107 42-43 -0.018018204439385954 1107 44-45 0.05769039255502406 1107 46-47 -0.007163252831418276 1107 48-49 -0.15082537480851954 1107 50-51 -0.16010996580061487 1107 52-53 -0.012476386459425726 1107 54-55 -0.15314126612674528 1107 56-57 0.026034116651828754 1107 58-59 -0.036623090876467757 1107 60-61 -0.1720937125814075 1107 62-63 -0.051178876434143206 1107 64-65 0.03736556050873574 1107 66-67 0.024445327033955522 1107 68-69 0.05947166348260424 1107 70-71 0.0606486325964255 1107 72-73 -0.28994516228592104 1107 74-75 -0.09265161534965571 1107 76-77 -0.1828506893954227 1107 78-79 -0.26056498363420033 1107 80-81 -0.20298061622983354 1107 82-83 -0.11707174503592199 1107 84-85 -0.06130986221264223 1107 86-87 -0.1925910113075524 1107 88-89 -0.26331003146797016 1107 90-91 -0.24579773207993227 1107 92-93 -0.24458113929883396 1107 94-95 -0.2340176497292923 1107 96-97 -0.27483167832367883 1107 98-99 -0.24501041080150898 1108 1 0.1521495034538276 1108 2 0.20172355592976032 1108 3 0.13455360687311924 1108 4 0.126649090084328 1108 5 0.0770588077822083 1108 6 0.11614376668529047 1108 7 0.24760540927312036 1108 8 0.13450101352858468 1108 9 0.14296254992856916 1108 10-11 0.10378928998208892 1108 12-13 0.1023012624629871 1108 14-15 0.06241771992752376 1108 16-17 0.08275042697625423 1108 18-19 0.33372312280613414 1108 20-21 0.27476815006491506 1108 22-23 0.14962741928406587 1108 24-25 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-0.5068858492329156 2216 8 -0.44809611214202505 2216 9 -0.3465794917420624 2216 10-11 -0.44514319787793966 2216 12-13 -0.5475189992131035 2216 14-15 -0.6199493582852256 2216 16-17 -0.5864458881038317 2216 18-19 -0.46623272505236457 2216 20-21 -0.3517025891592951 2216 22-23 -0.38196970493257254 2216 24-25 -0.5110755822868711 2216 26-27 -0.5753058786538787 2216 28-29 -0.6082903701121083 2216 30-31 -0.38398380688657596 2216 32-33 -0.3161561981017229 2216 34-35 -0.453142792397621 2216 36-37 -0.322412047716611 2216 38-39 -8.397926162141637 2216 40-41 -2.0921383759314978 2216 42-43 -0.7556198282637041 2216 44-45 -0.757159657203907 2216 46-47 -0.5661235193697394 2216 48-49 -0.43327512643621446 2216 50-51 -0.46726512315738233 2216 52-53 -0.5801505174429025 2216 54-55 -0.48817353665422303 2216 56-57 -0.39354360063963156 2216 58-59 -0.5561254344782327 2216 60-61 -0.6065410102749382 2216 62-63 -0.6167890168179717 2216 64-65 -0.7200332519437005 2216 66-67 -0.6311921038367316 2216 68-69 -0.6166300880195905 2216 70-71 -0.3947262516789998 2216 72-73 -0.46941853741333617 2216 74-75 -0.41205144334274024 2216 76-77 -0.6360980269383312 2216 78-79 -0.6598892411040005 2216 80-81 -0.5200877571261806 2216 82-83 -0.7235621572793605 2216 84-85 -0.6626054043013916 2216 86-87 -0.5559438361938369 2216 88-89 -0.4638322419136962 2216 90-91 -0.4907366012988703 2216 92-93 -0.4120674808577469 2216 94-95 -0.31117943306272267 2216 96-97 -0.2249036884968838 2216 98-99 -0.2438096316421614 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 169.0 8 1063.0 9 2209.0 10 4127.0 11 10930.0 12 28576.0 13 57194.0 14 77953.0 15 78014.0 16 76818.0 17 75895.0 18 73086.0 19 72162.0 20 72207.0 21 74734.0 22 79237.0 23 85154.0 24 91854.0 25 95616.0 26 96363.0 27 101108.0 28 117548.0 29 143005.0 30 176253.0 31 222571.0 32 291150.0 33 384891.0 34 510991.0 35 710679.0 36 1045482.0 37 1173250.0 38 340617.0 39 311.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.01307567877916 20.872994771172483 12.536519704709523 22.577409845338835 2 13.707664542797938 22.904789424960736 39.30785339623844 24.079692636002886 3 17.59814440624406 32.22100677922098 28.3630713988263 21.81777741570866 4 11.665728593810478 20.21194377589968 37.16110483113276 30.961222799157085 5 11.28265362149001 42.46610283090923 32.57355413360089 13.677689413999875 6 26.633635829130313 40.09228690652243 18.77542096346413 14.498656300883129 7 23.507811682105764 36.1650769255158 22.611758638101083 17.715352754277358 8 26.067825649663845 36.35300189069029 18.977219112577846 18.60195334706802 9 26.484260937233667 18.271796695702456 19.506866661916135 35.73707570514774 10-11 23.564473406646393 30.381186260205272 24.234400466096268 21.819939867052067 12-13 25.319862735639127 28.419242845491112 24.293432824646562 21.9674615942232 14-15 24.461387079442748 26.91833249856656 23.225992424157997 25.39428799783269 16-17 21.950013054833363 29.358315112678472 25.28127535088151 23.410396481606654 18-19 22.80344197922595 29.587694221104975 26.946111402874617 20.66275239679446 20-21 25.219016206948186 28.267507092050533 25.266503202370412 21.246973498630876 22-23 24.671381309747247 28.72868498844961 23.361285619977608 23.238648081825534 24-25 23.22007183625703 29.525387543485042 23.725655124438955 23.52888549581897 26-27 23.881180811634362 28.164048407340793 23.831958221698955 24.122812559325897 28-29 22.9477098310465 28.41196075655358 25.303887773261202 23.336441639138723 30-31 23.98077023591518 28.780637950256637 24.878925190011522 22.35966662381666 32-33 22.299723292430045 30.059495384400957 24.366723111066083 23.274058212102915 34-35 21.765555659844004 30.699114360864748 25.563032060745684 21.972297918545557 36-37 23.195231110320403 29.215468551855256 24.761825088026836 22.82747524979751 38-39 23.737726621687838 28.95382602741447 24.500827648160016 22.80761970273768 40-41 22.362935029567687 29.197917116296956 25.440525194338388 22.99862265979697 42-43 23.414971105097585 28.922416884875407 24.83619629793039 22.82641571209662 44-45 23.20638270521943 29.4534309536153 25.17683827124394 22.163348069921334 46-47 21.968084947756005 29.544142882547504 26.305087469901494 22.182684699795 48-49 22.89278891463578 29.338611659650603 26.088829416508936 21.679770009204685 50-51 21.414446957591643 29.86245914500833 25.324730820275075 23.398363077124948 52-53 20.893683420539652 30.198949380520308 23.861055232430157 25.046311966509887 54-55 21.37409204958926 29.8187332469239 23.70878692268888 25.09838778079796 56-57 23.271967716521157 28.11313971522878 23.778207961172683 24.83668460707738 58-59 22.145467318807803 28.389995445140947 25.96635368621824 23.498183549833016 60-61 22.85737290991396 29.079253246911225 24.688242058924768 23.37513178425005 62-63 21.79577791412025 28.93626502088849 24.623497793433618 24.644459271557636 64-65 20.731116239078524 29.503417116061403 25.21200999127004 24.553456653590036 66-67 23.84155147384396 28.27075860495055 24.29895863040915 23.588731290796343 68-69 23.32080076193681 28.044992632392486 24.596869127809626 24.037337477861076 70-71 22.430456695039087 29.030458892423887 24.30371938768303 24.23536502485399 72-73 24.152737374013242 28.253210667033322 24.772135324698727 22.821916634254713 74-75 24.46083963390995 28.22747527510905 24.833474857599384 22.478210233381624 76-77 23.024193681293458 28.689397883510022 25.53145721636192 22.754951218834606 78-79 23.45488436569152 28.722073968413515 25.26080619587563 22.562235470019335 80-81 22.710335763114685 28.867926038631857 25.796613771495498 22.62512442675796 82-83 23.316664831585456 28.134455393282753 25.335311715684263 23.213568059447528 84-85 23.778346972610894 28.634956804725277 24.151519427396764 23.435176795267065 86-87 23.36925215869242 27.677172559469792 25.119121649894886 23.834453631942903 88-89 24.25624111433638 26.175999628328523 25.56708936972491 24.000669887610186 90-91 24.14671006313824 27.06550287360494 25.148746028697833 23.639041034558993 92-93 23.713158143387965 27.02640217302249 25.576844176419645 23.6835955071699 94-95 22.370126088920518 27.62002493086879 26.277691329978037 23.732157650232654 96-97 23.60589173549127 27.586396602368286 24.852825902766682 23.954885759373763 98-99 23.58357236205372 28.339924739307747 24.159846879305697 23.916656019332837 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4858.0 1 3646.5 2 2260.0 3 1880.0 4 1458.0 5 1134.0 6 975.5 7 896.0 8 875.0 9 845.0 10 759.0 11 699.0 12 702.5 13 740.0 14 809.0 15 1167.0 16 1415.0 17 1504.5 18 1981.0 19 2582.0 20 3406.0 21 4677.5 22 6471.5 23 8844.5 24 12052.5 25 16764.0 26 22510.0 27 28529.0 28 38148.5 29 52941.5 30 65372.5 31 78368.5 32 92750.0 33 102741.0 34 111858.0 35 123488.0 36 127835.5 37 139308.5 38 159826.0 39 150136.0 40 136586.0 41 143543.5 42 150150.0 43 163559.0 44 198214.5 45 253584.0 46 291489.5 47 331845.5 48 372930.0 49 418450.0 50 483239.0 51 420104.5 52 303245.5 53 264964.5 54 217985.5 55 186922.5 56 161509.5 57 112482.0 58 99948.5 59 83567.5 60 54115.0 61 41663.5 62 31001.5 63 23009.0 64 13079.0 65 4794.5 66 3607.0 67 2971.0 68 2495.0 69 1975.0 70 1634.0 71 1508.5 72 1388.5 73 1282.5 74 1158.5 75 1234.5 76 1727.0 77 1815.5 78 1368.0 79 1331.5 80 1542.5 81 1376.5 82 1500.5 83 1437.5 84 927.5 85 755.5 86 468.5 87 210.0 88 131.5 89 119.5 90 123.5 91 103.5 92 77.0 93 53.0 94 28.5 95 23.0 96 28.5 97 20.5 98 10.0 99 9.0 100 10.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0021973820390386893 3 0.006702015219068003 4 0.0 5 0.005210934549720321 6 0.0 7 8.632572296223422E-4 8 0.0 9 0.0 10-11 3.139117198626699E-5 12-13 1.334124809416347E-4 14-15 0.0027781187207846286 16-17 2.2758599690043567E-4 18-19 0.0 20-21 0.0 22-23 6.278234397253398E-5 24-25 9.731263315742768E-4 26-27 0.0025034459659047923 28-29 0.0018206879752034853 30-31 0.0024642070009219586 32-33 8.083226786463749E-4 34-35 0.0 36-37 1.1771689494850122E-4 38-39 0.05929792388205834 40-41 0.0038140273963314392 42-43 3.9238964982833734E-4 44-45 1.5695585993133495E-5 46-47 8.632572296223422E-4 48-49 1.0202130895536772E-4 50-51 8.868006086120425E-4 52-53 3.845418568317706E-4 54-55 0.0 56-57 5.258021307699721E-4 58-59 0.0 60-61 1.4126027393820145E-4 62-63 1.0986910195193446E-4 64-65 8.789528156154757E-4 66-67 0.001396907153388881 68-69 0.0013498203954094806 70-71 1.2556468794506796E-4 72-73 0.0017579056312309513 74-75 0.0016951232872584176 76-77 1.4126027393820145E-4 78-79 1.8834703191760195E-4 80-81 0.0 82-83 3.0606392686610316E-4 84-85 5.493455097596723E-4 86-87 0.0 88-89 0.0 90-91 6.19975646728773E-4 92-93 0.0 94-95 0.0 96-97 0.003013552510681631 98-99 0.0046458934539675145 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 6371218.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.97646337497515 #Duplication Level Percentage of deduplicated Percentage of total 1 78.7046353661344 16.509449011984916 2 9.812738413620828 4.116730958830581 3 3.869077741092058 2.434787025928474 4 1.9597263913844638 1.6443251549539368 5 1.185002712550875 1.2428582999534816 6 0.7618831533751909 0.9588968437671158 7 0.5025556068230839 0.737928749628898 8 0.3802730187223643 0.6381426439776731 9 0.28377138649525413 0.5357268086115254 >10 1.8355371565510117 7.6421705336441645 >50 0.26888712709234536 4.0055733781525635 >100 0.30933343415380526 14.134269800342041 >500 0.0639417124561301 9.38131302907185 >1k 0.05734028716002968 23.051134750229078 >5k 0.0038380379625187204 5.519660206314017 >10k+ 0.0014584544257571137 7.447032804609781 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 80406 1.2620192873638918 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 75048 1.1779223376126826 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 31798 0.4990882434096589 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 26903 0.42225834997327044 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 22780 0.35754544892358103 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 22376 0.3512044321823551 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 22354 0.35085912929050617 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 21514 0.337674837056274 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 21477 0.3370941003745281 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 20746 0.3256206270135475 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 17972 0.2820810714685952 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 15589 0.24467849004695805 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 14478 0.22724069400858674 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 13795 0.21652060877527657 No Hit GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC 12706 0.1994281156287542 No Hit GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC 11429 0.1793848523155227 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 10867 0.1705639329873817 No Hit GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA 10247 0.16083266967163892 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 10015 0.15719129372123194 No Hit CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA 9977 0.15659486145349288 No Hit GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT 9501 0.14912376252076134 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 9489 0.14893541548884373 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 9464 0.14854302583901538 No Hit GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG 9425 0.1479308979852832 No Hit GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT 9311 0.14614160118206598 No Hit ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA 9108 0.14295539722545988 No Hit GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT 8854 0.13896871838320396 No Hit CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC 8555 0.13427573817125707 No Hit ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA 8124 0.1275109406082165 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 7825 0.1228179603962696 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 7821 0.12275517805229706 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 7807 0.1225354398483932 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 7735 0.12140535765688759 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 7508 0.11784245963644628 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 7476 0.117340200884666 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 7447 0.11688502889086515 No Hit CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA 7183 0.11274139418867789 No Hit GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT 7139 0.11205078840498 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 6968 0.10936684320015419 No Hit CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT 6950 0.1090843226522778 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 6890 0.10814258749268979 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 6802 0.10676137592529403 No Hit ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG 6783 0.1064631597914245 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 6420 0.10076566207591704 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.5695585993133495E-5 0.0 0.0 0.0 0.0 2 1.5695585993133495E-5 0.0 0.0 0.0 0.0 3 1.5695585993133495E-5 0.0 0.0 0.0 0.0 4 1.5695585993133495E-5 0.0 0.0 1.5695585993133495E-5 0.0 5 3.139117198626699E-5 0.0 0.0 3.139117198626699E-5 0.0 6 3.139117198626699E-5 0.0 0.0 3.139117198626699E-5 0.0 7 3.139117198626699E-5 0.0 0.0 3.139117198626699E-5 0.0 8 3.139117198626699E-5 0.0 0.0 3.139117198626699E-5 0.0 9 3.139117198626699E-5 0.0 0.0 3.139117198626699E-5 0.0 10-11 3.923896498283374E-5 0.0 0.0 4.708675797940049E-5 0.0 12-13 6.278234397253398E-5 0.0 0.0 7.847792996566748E-5 0.0 14-15 7.847792996566748E-5 0.0 0.0 1.2556468794506796E-4 0.0 16-17 1.0202130895536771E-4 0.0 0.0 1.5695585993133495E-4 0.0 18-19 1.648036529279017E-4 0.0 0.0 2.5897716888670266E-4 0.0 20-21 2.511293758901359E-4 0.0 0.0 6.435190257184733E-4 0.0 22-23 2.668249618832694E-4 0.0 0.0 0.0030998782336438655 0.0 24-25 2.9036834087296966E-4 0.0 0.0 0.009770502280725601 0.0 26-27 3.923896498283374E-4 0.0 0.0 0.016762885840666573 0.0 28-29 4.787153727905716E-4 0.0 0.0 0.03712006087376071 0.0 30-31 5.650410957528058E-4 0.0 0.0 0.09813665142206718 0.0 32-33 5.964322677390728E-4 0.0 0.0 0.19670493145894552 0.0 34-35 6.356712327219066E-4 0.0 0.0 0.32015071529494044 0.0 36-37 6.906057836978738E-4 0.0 0.0 0.5174442312286285 0.0 38-39 7.219969556841408E-4 0.0 0.0 0.8417621246047459 0.0 40-41 7.376925416772743E-4 0.0 0.0 1.2734770651388794 0.0 42-43 8.08322678646375E-4 0.0 0.0 1.6105868610994005 0.0 44-45 8.475616436292088E-4 0.0 0.0 1.9572788123087297 0.0 46-47 8.632572296223423E-4 0.0 0.0 2.322350294716018 0.0 48-49 8.789528156154757E-4 0.0 0.0 2.722885953674792 0.0 50-51 8.946484016086092E-4 0.0 0.0 3.209558988563882 0.0 52-53 8.946484016086092E-4 0.0 0.0 3.6914605653110595 0.0 54-55 9.103439876017427E-4 0.0 0.0 4.102888960949068 0.0 56-57 9.260395735948762E-4 0.0 0.0 4.546658111525928 0.0 58-59 9.260395735948762E-4 0.0 0.0 5.044082622820314 0.0 60-61 9.574307455811432E-4 0.0 0.0 5.53115589515223 0.0 62-63 9.809741245708436E-4 0.0 0.0 6.028038908729854 0.0 64-65 9.888219175674103E-4 0.0 0.0 6.539095036459276 0.0 66-67 9.96669710563977E-4 0.0 0.0 7.059223212892731 0.0 68-69 0.0010045175035605437 0.0 0.0 7.635266914426723 0.0 70-71 0.0010045175035605437 0.0 0.0 8.347752344999027 0.0 72-73 0.0010045175035605437 0.0 0.0 8.978589337235046 0.0 74-75 0.0010045175035605437 0.0 0.0 9.564026846985929 0.0 76-77 0.0010045175035605437 0.0 0.0 10.181075266926984 0.0 78-79 0.0010045175035605437 0.0 0.0 10.781713951712216 0.0 80-81 0.0010045175035605437 0.0 0.0 11.448438901321538 0.0 82-83 0.0010045175035605437 3.139117198626699E-5 0.0 12.214312867649483 0.0 84-85 0.0010045175035605437 5.493455097596723E-5 0.0 12.91370817950351 0.0 86-87 0.0010045175035605437 6.278234397253398E-5 0.0 13.586287582688271 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATA 1860 0.0 48.499035 1 TACATGA 2510 0.0 45.202286 2 GTACAAA 4050 0.0 34.673386 1 GTACAAG 3960 0.0 34.639458 1 TACATAA 1995 0.0 32.864006 2 TACATAG 1475 0.0 32.785786 2 TCAAGAC 975 0.0 31.476297 3 TATTCGC 760 0.0 30.590296 9 TACAAGA 2115 0.0 30.33982 2 ACGTTAA 970 0.0 30.197584 8 GTACTAG 6305 0.0 29.868555 1 AGGGCTA 3335 0.0 29.138292 5 TAGGCAT 8450 0.0 28.282646 5 TTAAGGT 1155 0.0 27.374146 4 ACATGAG 2300 0.0 26.888596 3 TATTAAG 1370 0.0 26.813334 2 GTAACAA 1945 0.0 26.775816 1 CTATTGA 3690 0.0 26.209831 9 GAACAAA 3520 0.0 25.89153 1 CTAGGCA 9430 0.0 25.787167 4 >>END_MODULE