FastQCFastQC Report
Fri 27 May 2016
ERR765541_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765541_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12165308
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT292240.2402240863938669No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA282300.23205331093959972No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166060.13650291468165046No Hit
GTTATATACTTTAATATTATGCTTTGAGAATTAAGGCCATAGTGCACACA126200.1037376119042773No Hit
CTTTAATATTATGCTTTGAGAATTAAGGCCATAGTGCACACAGAGTTCCT123230.1012962433832337No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATAT60150.033.1362461
ATATACT68050.030.4564954
TTATATA69200.029.6821142
TATACTT99650.023.8155355
TATATAC133650.023.3500753
GAGTTAT26300.022.8784871
TACTTTA89250.022.1653967
ATACTTT100050.020.9479166
TATTATG84000.020.4740317
TAGTACT44550.019.83264
GTACTAG34700.019.5077021
TAATATT101550.018.6043974
CATGGGG278650.018.6031274
GTATAAG42000.018.3555581
ATATTAT95500.018.2062876
ATTATGC98250.018.0294978
CTAGTAC46800.017.4740073
GTACATG955100.017.3985731
AATATTA101050.017.1607515
GTATATA97850.017.1506441
Produced by FastQC (version 0.11.5)