##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765541_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12165308
Sequences flagged as poor quality	0
Sequence length	100
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.77677671621631	33.0	33.0	33.0	27.0	33.0
2	31.671396318120348	33.0	33.0	33.0	27.0	33.0
3	31.640199902871345	33.0	33.0	33.0	27.0	33.0
4	35.26771685517539	37.0	37.0	37.0	33.0	37.0
5	35.4716650001792	37.0	37.0	37.0	33.0	37.0
6	35.46520795034536	37.0	37.0	37.0	33.0	37.0
7	35.34619255015985	37.0	37.0	37.0	33.0	37.0
8	35.321010532573446	37.0	37.0	37.0	33.0	37.0
9	35.02600106795487	37.0	37.0	37.0	33.0	37.0
10-11	35.04090274574224	37.0	37.0	37.0	33.0	37.0
12-13	35.015685916049144	37.0	37.0	37.0	33.0	37.0
14-15	36.33613057721186	37.0	37.0	40.0	30.0	40.0
16-17	36.593802803841875	37.0	37.0	40.0	33.0	40.0
18-19	36.40712503949756	37.0	37.0	40.0	33.0	40.0
20-21	36.39971293780643	37.0	37.0	40.0	33.0	40.0
22-23	36.58452815169168	37.0	37.0	40.0	33.0	40.0
24-25	36.60375261357953	37.0	37.0	40.0	33.0	40.0
26-27	36.27690396330286	37.0	37.0	40.0	30.0	40.0
28-29	36.15938753872898	37.0	37.0	40.0	27.0	40.0
30-31	35.97057028889034	37.0	37.0	40.0	27.0	40.0
32-33	35.945706389020316	37.0	37.0	40.0	27.0	40.0
34-35	35.89718669679387	37.0	37.0	40.0	27.0	40.0
36-37	35.67030210003725	37.0	37.0	40.0	27.0	40.0
38-39	35.54463134020117	37.0	37.0	40.0	27.0	40.0
40-41	35.70894251094999	37.0	37.0	40.0	27.0	40.0
42-43	35.6408346997873	37.0	37.0	40.0	27.0	40.0
44-45	35.600727988144655	37.0	37.0	40.0	27.0	40.0
46-47	35.574770322296814	37.0	33.0	40.0	27.0	40.0
48-49	35.423897528940486	37.0	33.0	40.0	27.0	40.0
50-51	35.28479439238201	37.0	33.0	40.0	27.0	40.0
52-53	35.17314950020172	37.0	33.0	40.0	27.0	40.0
54-55	35.05762159905857	37.0	33.0	40.0	27.0	40.0
56-57	34.86494937078453	37.0	33.0	40.0	27.0	40.0
58-59	34.68743097174358	37.0	33.0	40.0	27.0	40.0
60-61	34.414106654759586	37.0	33.0	38.5	27.0	40.0
62-63	34.18071959213856	37.0	33.0	37.0	27.0	40.0
64-65	33.98844636732584	37.0	33.0	37.0	27.0	40.0
66-67	33.771522513034604	37.0	33.0	37.0	27.0	40.0
68-69	33.5609492994341	37.0	33.0	37.0	27.0	40.0
70-71	33.31814599350875	37.0	33.0	37.0	27.0	38.5
72-73	33.06960206021911	37.0	33.0	37.0	27.0	37.0
74-75	32.84035287063838	37.0	33.0	37.0	24.5	37.0
76-77	32.63006592188213	35.0	33.0	37.0	22.0	37.0
78-79	32.43358828235175	33.0	33.0	37.0	22.0	37.0
80-81	32.28373539741041	33.0	33.0	37.0	22.0	37.0
82-83	32.13963325055148	33.0	33.0	37.0	22.0	37.0
84-85	31.977560288650317	33.0	33.0	37.0	22.0	37.0
86-87	31.768720364498783	33.0	33.0	37.0	22.0	37.0
88-89	31.63045021137155	33.0	33.0	37.0	22.0	37.0
90-91	31.542986252382594	33.0	33.0	37.0	22.0	37.0
92-93	31.4577294302783	33.0	33.0	37.0	22.0	37.0
94-95	31.318718605398235	33.0	33.0	37.0	22.0	37.0
96-97	31.217066472957363	33.0	33.0	37.0	22.0	37.0
98-99	31.097466295140247	33.0	27.0	37.0	22.0	37.0
100	31.013510303232767	33.0	27.0	37.0	15.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	5.0
7	171.0
8	1108.0
9	2254.0
10	3666.0
11	5955.0
12	10631.0
13	17080.0
14	25087.0
15	31178.0
16	36110.0
17	42485.0
18	51363.0
19	61655.0
20	73024.0
21	86650.0
22	100281.0
23	106399.0
24	111299.0
25	123080.0
26	144197.0
27	174775.0
28	218321.0
29	273107.0
30	344521.0
31	429596.0
32	554383.0
33	741315.0
34	990690.0
35	1357899.0
36	2024753.0
37	2882844.0
38	1138374.0
39	1051.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.602800156587705	18.565816180510094	12.810584802439609	25.02079886046259
2	15.748257700346988	20.93366804868852	37.5840769244557	25.73399732650879
3	18.169011560057758	25.76530029166121	29.38866087926555	26.67702726901549
4	12.613252662171368	17.342970614574416	36.595018773903135	33.44875794935108
5	14.089161830321908	37.19881915139908	33.66551414334874	15.046504874930283
6	32.021018114133454	36.36815032324144	17.2629601823541	14.347871380271005
7	27.542445713255592	31.38143828312779	22.346759473770696	18.729356529845926
8	27.244939397725854	32.396699921739874	20.32050481069599	20.037855869838282
9	26.62022120371021	14.550319548895358	19.739009379928408	39.09044986746602
10-11	24.985817968061102	25.14073729796718	27.37567427845085	22.497770455520865
12-13	26.497815481107917	22.82593720587087	26.522739135974483	24.153508177046728
14-15	24.004852438559993	23.81637346596501	24.637071011200764	27.541703084274232
16-17	23.62815599350684	26.525761224665867	25.480744430501773	24.36533835132552
18-19	24.41416198909146	25.626852210243428	26.888729876746957	23.070255923918157
20-21	25.616850049501565	24.950282635336418	26.189827725662905	23.243039589499105
22-23	24.671792697287056	24.79425343041125	26.005221127127932	24.528732745173766
24-25	24.179183655900786	25.460198048278375	25.901251796904294	24.459366498916545
26-27	24.618511655863124	25.341157491363404	25.89028199118477	24.150048861588708
28-29	24.515069655289164	24.811778107193415	26.364930219709983	24.30822201780744
30-31	24.605296966456887	25.059081125112677	26.29881128879068	24.036810619639756
32-33	24.317508135808925	25.268057778839875	25.81659887579707	24.59783520955413
34-35	24.64303028782833	25.11168834586605	26.247326679827836	23.99795468647778
36-37	24.79641084226036	25.24044201387608	25.729942516945837	24.233204626917722
38-39	24.879820276321453	24.829473466042533	25.833543402996956	24.45716285463906
40-41	24.336313808933827	25.185555465224407	26.0067067383323	24.471423987509464
42-43	25.214413883431835	25.010035309567247	25.68848433371076	24.08706647329016
44-45	24.805661484607832	25.359962067049697	25.080621912858668	24.7537545354838
46-47	24.185301705083344	25.26900393089123	26.04689111828984	24.498803245735584
48-49	24.874404120273628	25.63574418802635	25.27626076038181	24.213590931318215
50-51	24.426225956188656	25.855849147924122	24.950931081167365	24.766993814719857
52-53	24.396611078997882	25.64786382369246	24.969725336547725	24.98579976076193
54-55	24.516765214657944	25.566426267218223	25.080129496104824	24.836679022019005
56-57	24.64524942566189	24.98437770749413	24.861105037373488	25.509267829470488
58-59	24.2750080803544	25.070310591396456	25.459466377670008	25.195214950579135
60-61	24.728917673107826	25.16976553326887	25.149046781224115	24.952270012399193
62-63	23.849403649004724	25.53534724154527	25.175876175018402	25.4393729344316
64-65	24.168748202366125	25.110102120811355	25.138140893724177	25.58300878309835
66-67	24.660795831479867	25.233347552766265	24.886495592599147	25.21936102315471
68-69	24.68769339282066	25.048477544721226	25.01799296902464	25.24583609343348
70-71	24.12163567077876	24.67442943129253	25.327734511033086	25.876200386895626
72-73	24.732452495195442	24.86348535065335	25.280709975229072	25.123352178922136
74-75	24.804761344425152	25.101437050049757	25.159006513894205	24.934795091630882
76-77	24.56502929397067	25.012383580777524	25.370077125911056	25.05250999934075
78-79	24.606512223118397	25.189912988639502	25.249767617885222	24.953807170356885
80-81	24.209779368458086	24.9968578675175	25.70118874948059	25.092174014543822
82-83	24.457184239507598	24.898287484661797	25.076437742865615	25.568090532964987
84-85	24.2634668095007	25.132912262288524	24.846463279422036	25.757157648788738
86-87	24.160863156689363	24.867642076406042	25.251165521213544	25.720329245691055
88-89	24.37356560842042	24.42772507786888	25.31920465933377	25.879504654376927
90-91	24.455832095372937	24.503066605632817	25.29907003667004	25.7420312623242
92-93	24.347852249305642	24.698732240111937	25.274341806137574	25.67907370444485
94-95	23.701749365071713	24.830355949073883	25.719027437541598	25.748867248312806
96-97	24.15629643177881	24.704885522224572	25.211584028803884	25.92723401719273
98-99	24.221785873013282	24.766861598937297	25.128432812855856	25.882919715193566
100	23.730512661375506	25.05465291678141	25.261139585448355	25.953694836394725
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	875.0
1	904.0
2	1308.0
3	1649.0
4	1459.5
5	1359.5
6	1645.5
7	2842.5
8	4887.5
9	5027.5
10	3251.5
11	2241.0
12	2031.5
13	2085.5
14	2392.5
15	2874.0
16	3383.5
17	4090.5
18	5198.5
19	6579.0
20	8108.5
21	10027.5
22	12402.5
23	15157.0
24	18566.0
25	22902.0
26	29167.0
27	36523.5
28	44115.5
29	55390.5
30	68403.0
31	80561.5
32	95297.0
33	109771.5
34	124028.5
35	137267.5
36	154008.0
37	177109.0
38	191033.5
39	202185.0
40	227637.5
41	250895.5
42	264027.5
43	294163.0
44	326895.0
45	357301.5
46	415505.5
47	477354.5
48	529003.0
49	568632.0
50	630745.0
51	658427.5
52	623137.5
53	582714.0
54	539411.5
55	506714.0
56	472489.0
57	439427.0
58	414606.0
59	373886.5
60	316885.5
61	260529.5
62	211563.0
63	165996.5
64	123072.5
65	95244.0
66	75117.0
67	57371.0
68	44919.0
69	37129.5
70	32469.0
71	29199.5
72	25335.5
73	23828.5
74	18935.5
75	13652.5
76	10001.5
77	6929.0
78	5077.0
79	3508.0
80	2532.5
81	1731.5
82	1168.0
83	834.5
84	568.5
85	367.0
86	265.5
87	164.5
88	86.0
89	51.0
90	38.5
91	33.0
92	24.0
93	11.5
94	6.0
95	5.5
96	4.0
97	3.0
98	1.5
99	1.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0492219350303338
2	0.024117761753339906
3	0.021092766414134358
4	0.02202985736160564
5	0.015223617848393153
6	0.024964431644476242
7	0.04844924600347151
8	0.0061486318307764996
9	0.004134708303316283
10-11	0.0021947656401301143
12-13	0.0012987751728110789
14-15	0.0023879378968456864
16-17	0.006695268216801415
18-19	0.005084129394833242
20-21	0.00860644054388101
22-23	0.01829382371576618
24-25	0.025248024957526764
26-27	0.027972986791620896
28-29	0.04431864774817045
30-31	0.04209922181994899
32-33	0.040031867668290845
34-35	0.04770121726470057
36-37	0.04491049466236284
38-39	0.05460609793027846
40-41	0.05213595907312828
42-43	0.05744614110879889
44-45	0.05764753346154491
46-47	0.06273988295240861
48-49	0.0646346150874273
50-51	0.033673623388737875
52-53	1.6440192060899732E-4
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	6.000670102228403E-4
64-65	9.042105633494853E-5
66-67	2.589330249591708E-4
68-69	0.0011467033962477564
70-71	9.453110435017346E-5
72-73	3.164736971723198E-4
74-75	3.6990432137024396E-5
76-77	4.93205761826992E-5
78-79	0.0
80-81	2.46602880913496E-5
82-83	1.2741148847197293E-4
84-85	0.0011508134442629813
86-87	0.003875775278357112
88-89	0.0025235694813481088
90-91	0.0038922154704180115
92-93	0.003822344654159188
94-95	0.002889363754703128
96-97	0.002326287176617312
98-99	0.00398263652675296
100	0.002942794378901052
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.2165308E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.88567562644214
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.53708638606824	28.23887876962678
2	13.14394218120356	9.959342598595772
3	4.861377370737986	5.525296984965165
4	2.237360012380255	3.3905558275444365
5	1.2337734272221252	2.337116993013063
6	0.7704456731221725	1.7513312915821497
7	0.5242558951321938	1.3903252151759855
8	0.3887267430994711	1.1781740237111895
9	0.2850094891055749	0.9717999349240624
>10	1.6933629012402114	11.937396638578866
>50	0.1549275739859442	4.083285921717126
>100	0.13349063412209178	10.484865346218083
>500	0.020679443764087514	5.429151699351463
>1k	0.014696433099072086	10.472927923420858
>5k	7.291248146486632E-4	1.899668902586692
>10k+	1.3671090274662437E-4	0.94988192898841
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	29224	0.2402240863938669	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	28230	0.23205331093959972	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16606	0.13650291468165046	No Hit
GTTATATACTTTAATATTATGCTTTGAGAATTAAGGCCATAGTGCACACA	12620	0.1037376119042773	No Hit
CTTTAATATTATGCTTTGAGAATTAAGGCCATAGTGCACACAGAGTTCCT	12323	0.1012962433832337	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.220096030449866E-6
2	1.644019206089973E-5	8.220096030449866E-6	0.0	8.220096030449866E-6	8.220096030449866E-6
3	1.644019206089973E-5	8.220096030449866E-6	0.0	8.220096030449866E-6	8.220096030449866E-6
4	1.644019206089973E-5	2.46602880913496E-5	0.0	8.220096030449866E-6	8.220096030449866E-6
5	1.644019206089973E-5	2.46602880913496E-5	0.0	2.46602880913496E-5	8.220096030449866E-6
6	1.644019206089973E-5	2.46602880913496E-5	0.0	2.46602880913496E-5	8.220096030449866E-6
7	1.644019206089973E-5	2.46602880913496E-5	0.0	2.46602880913496E-5	8.220096030449866E-6
8	2.46602880913496E-5	2.46602880913496E-5	0.0	2.46602880913496E-5	8.220096030449866E-6
9	2.46602880913496E-5	4.93205761826992E-5	0.0	2.46602880913496E-5	1.644019206089973E-5
10-11	2.46602880913496E-5	4.93205761826992E-5	0.0	4.110048015224933E-5	1.644019206089973E-5
12-13	2.877033610657453E-5	4.93205761826992E-5	0.0	4.93205761826992E-5	1.644019206089973E-5
14-15	3.288038412179946E-5	5.7540672213149064E-5	0.0	4.93205761826992E-5	1.644019206089973E-5
16-17	3.288038412179946E-5	5.7540672213149064E-5	0.0	6.576076824359893E-5	1.644019206089973E-5
18-19	3.288038412179946E-5	8.63110083197236E-5	0.0	7.809091228927373E-5	1.644019206089973E-5
20-21	3.288038412179946E-5	1.2330144045674798E-4	0.0	1.84952160685122E-4	3.288038412179946E-5
22-23	4.5210528167474264E-5	1.4796172854809758E-4	0.0	8.631100831972359E-4	4.110048015224933E-5
24-25	4.93205761826992E-5	1.726220166394472E-4	0.0	0.0021783254480692146	4.93205761826992E-5
26-27	6.987081625882386E-5	1.8906220870034692E-4	0.0	0.003933315950570261	4.93205761826992E-5
28-29	7.398086427404879E-5	1.9317225671557184E-4	0.0	0.012560306734527394	4.93205761826992E-5
30-31	9.86411523653984E-5	2.4660288091349597E-4	0.0	0.040307240885310916	4.93205761826992E-5
32-33	9.86411523653984E-5	2.5482297694394586E-4	0.0	0.08918393188236583	6.576076824359893E-5
34-35	9.86411523653984E-5	3.0414355312664506E-4	0.0	0.15244168088469276	6.576076824359893E-5
36-37	1.0686124839584826E-4	3.5346412930934427E-4	0.0	0.25204047443763855	6.576076824359893E-5
38-39	1.0686124839584826E-4	3.69904321370244E-4	0.0	0.4064385381775784	6.576076824359893E-5
40-41	1.109712964110732E-4	4.110048015224933E-4	0.0	0.5929977276366534	6.987081625882386E-5
42-43	1.3152153648719785E-4	4.2333494556816807E-4	0.0	0.7635893805565794	7.398086427404879E-5
44-45	1.3152153648719785E-4	4.849856657965421E-4	0.0	0.9594989292502911	7.398086427404879E-5
46-47	1.3152153648719785E-4	5.301961939640164E-4	0.0	1.1796331009457386	8.220096030449866E-5
48-49	1.3152153648719785E-4	5.384162899944663E-4	0.0	1.403075861293442	1.0275120038062333E-4
50-51	1.3152153648719785E-4	5.877368661771654E-4	0.0	1.6453467515988909	1.0686124839584826E-4
52-53	1.3152153648719785E-4	6.082871062532901E-4	0.0	1.8927634220194014	1.2330144045674798E-4
54-55	1.3152153648719785E-4	6.1650720228374E-4	0.0	2.1317298337206094	1.4385168053287264E-4
56-57	1.3152153648719785E-4	6.247272983141898E-4	0.0	2.4030341032056075	1.5207177656332253E-4
58-59	1.3152153648719785E-4	6.247272983141898E-4	0.0	2.7023730102024546	1.5618182457854745E-4
60-61	1.3152153648719785E-4	6.576076824359893E-4	0.0	3.00396011346363	1.6440192060899732E-4
62-63	1.3152153648719785E-4	6.78157922512114E-4	0.0	3.316085379835841	1.726220166394472E-4
64-65	1.3152153648719785E-4	7.480287387709378E-4	0.0	3.642616364501417	1.726220166394472E-4
66-67	1.3152153648719785E-4	7.562488348013877E-4	0.0	3.9971614364387653	1.767320646546721E-4
68-69	1.3152153648719785E-4	7.562488348013877E-4	0.0	4.390558792264034	1.8084211266989705E-4
70-71	1.3974163251764772E-4	7.644689308318375E-4	0.0	4.832483485005065	1.8084211266989705E-4
72-73	1.3974163251764772E-4	7.809091228927372E-4	0.0	5.265768856818093	1.972823047307968E-4
74-75	1.4385168053287264E-4	8.261196510602115E-4	0.0	5.690628630199909	1.972823047307968E-4
76-77	1.4796172854809758E-4	8.959904673190354E-4	0.0	6.138566323187214	2.0139235274602174E-4
78-79	1.4796172854809758E-4	9.124306593799352E-4	0.0	6.618360998340528	2.0550240076124666E-4
80-81	1.4796172854809758E-4	9.124306593799352E-4	0.0	7.12940025850558	2.0550240076124666E-4
82-83	1.4796172854809758E-4	9.124306593799352E-4	0.0	7.6751283239191315	2.0550240076124666E-4
84-85	1.4796172854809758E-4	9.412009954865096E-4	0.0	8.218567092588202	2.0550240076124666E-4
86-87	1.4796172854809758E-4	9.494210915169596E-4	0.0	8.774364775639054	2.0550240076124666E-4
88	1.5618182457854745E-4	0.0010110718117453336	0.0	9.20924484608199	2.0550240076124666E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTATAT	6015	0.0	33.136246	1
ATATACT	6805	0.0	30.456495	4
TTATATA	6920	0.0	29.682114	2
TATACTT	9965	0.0	23.815535	5
TATATAC	13365	0.0	23.350075	3
GAGTTAT	2630	0.0	22.878487	1
TACTTTA	8925	0.0	22.165396	7
ATACTTT	10005	0.0	20.947916	6
TATTATG	8400	0.0	20.474031	7
TAGTACT	4455	0.0	19.8326	4
GTACTAG	3470	0.0	19.507702	1
TAATATT	10155	0.0	18.604397	4
CATGGGG	27865	0.0	18.603127	4
GTATAAG	4200	0.0	18.355558	1
ATATTAT	9550	0.0	18.206287	6
ATTATGC	9825	0.0	18.029497	8
CTAGTAC	4680	0.0	17.474007	3
GTACATG	95510	0.0	17.398573	1
AATATTA	10105	0.0	17.160751	5
GTATATA	9785	0.0	17.150644	1
>>END_MODULE