FastQCFastQC Report
Fri 27 May 2016
ERR765537_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765537_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6891706
Sequences flagged as poor quality0
Sequence length99
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA131550.1908816191520648No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT118340.17171365116271647No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG9450.022.1425085
GTACTAG18700.021.8824831
GTTATAT19000.021.2922331
TATTCGC3850.020.5321439
ATGGGTA36700.020.2721585
TATATAC33700.019.1792223
GTATAAG25600.018.5274521
TAGGCAT30200.018.3225575
TATACTT29750.018.2871065
GTATCAA294550.018.2338541
CATGGGG149150.018.175714
ATATACT25400.018.123734
GTACAAG28950.017.6684991
GTACATG447100.017.6599121
TACATGG438600.017.0900172
TAGTACT25650.017.0406654
GTATATG26200.017.0382671
GTATTAT15850.017.0158731
CGTTAAC5200.016.9904861
GTAATAC11350.016.797093
Produced by FastQC (version 0.11.5)