##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765537_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6891706 Sequences flagged as poor quality 0 Sequence length 99 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.691721324153992 33.0 33.0 33.0 27.0 33.0 2 31.52632729834964 33.0 33.0 33.0 27.0 33.0 3 31.60136038885002 33.0 33.0 33.0 27.0 33.0 4 35.26501188530097 37.0 37.0 37.0 33.0 37.0 5 35.34342846314106 37.0 37.0 37.0 33.0 37.0 6 35.34057764507076 37.0 37.0 37.0 33.0 37.0 7 35.216686115165096 37.0 37.0 37.0 33.0 37.0 8 35.14179406376302 37.0 37.0 37.0 33.0 37.0 9 35.10140508025154 37.0 37.0 37.0 33.0 37.0 10-11 35.081706184216216 37.0 37.0 37.0 33.0 37.0 12-13 35.03725195183892 37.0 37.0 37.0 33.0 37.0 14-15 36.5665947589755 40.0 37.0 40.0 33.0 40.0 16-17 36.60997697812414 38.5 37.0 40.0 33.0 40.0 18-19 36.60957453785754 38.5 37.0 40.0 33.0 40.0 20-21 36.608522838902296 37.0 37.0 40.0 33.0 40.0 22-23 36.63810020334588 37.0 37.0 40.0 33.0 40.0 24-25 36.59579043273175 37.0 37.0 40.0 33.0 40.0 26-27 36.60785377379709 37.0 37.0 40.0 33.0 40.0 28-29 36.52751089498014 37.0 37.0 40.0 33.0 40.0 30-31 36.49902244814274 37.0 37.0 40.0 33.0 40.0 32-33 36.37991826697192 37.0 37.0 40.0 33.0 40.0 34-35 36.332560544515395 37.0 37.0 40.0 33.0 40.0 36-37 36.22755345338295 37.0 37.0 40.0 30.0 40.0 38-39 36.03082661970781 37.0 37.0 40.0 27.0 40.0 40-41 35.95845789126814 37.0 37.0 40.0 27.0 40.0 42-43 35.82775962874795 37.0 37.0 40.0 27.0 40.0 44-45 35.69246119901226 37.0 37.0 40.0 27.0 40.0 46-47 35.567693616065455 37.0 33.0 40.0 27.0 40.0 48-49 35.40988849495321 37.0 33.0 40.0 27.0 40.0 50-51 35.24719126149607 37.0 33.0 40.0 27.0 40.0 52-53 35.07129134643875 37.0 33.0 40.0 27.0 40.0 54-55 34.93406066654613 37.0 33.0 40.0 27.0 40.0 56-57 34.74225032234399 37.0 33.0 40.0 27.0 40.0 58-59 34.5243361367998 37.0 33.0 38.5 27.0 40.0 60-61 34.291473765712 37.0 33.0 37.0 27.0 40.0 62-63 34.11288605462856 37.0 33.0 37.0 27.0 40.0 64-65 33.811639744933984 37.0 33.0 37.0 27.0 40.0 66-67 33.58290030073831 37.0 33.0 37.0 27.0 40.0 68-69 33.28658651428253 37.0 33.0 37.0 27.0 40.0 70-71 33.13451799888155 37.0 33.0 37.0 27.0 37.0 72-73 32.86704199221499 37.0 33.0 37.0 22.0 37.0 74-75 32.60211237972136 33.0 33.0 37.0 22.0 37.0 76-77 32.42003576472937 33.0 33.0 37.0 22.0 37.0 78-79 32.19259433005412 33.0 33.0 37.0 22.0 37.0 80-81 31.942790000037725 33.0 33.0 37.0 22.0 37.0 82-83 31.780113080854 33.0 33.0 37.0 22.0 37.0 84-85 31.611490899350613 33.0 33.0 37.0 22.0 37.0 86-87 31.417909513261304 33.0 33.0 37.0 22.0 37.0 88-89 31.257053405934613 33.0 30.0 37.0 22.0 37.0 90-91 31.074762112603178 33.0 27.0 37.0 22.0 37.0 92-93 30.945034001740645 33.0 27.0 37.0 22.0 37.0 94-95 30.718763031969154 33.0 27.0 37.0 15.0 37.0 96-97 30.641022193924115 33.0 27.0 37.0 15.0 37.0 98-99 29.48042204644249 33.0 27.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2361354248920371 1101 2 0.24450441104043463 1101 3 0.27471612841532433 1101 4 0.2951079838558144 1101 5 0.29536252535288554 1101 6 0.2871805898423645 1101 7 0.19804428675832497 1101 8 0.2622739656881947 1101 9 0.22034586668265632 1101 10-11 0.23161397059165267 1101 12-13 0.1815255759655372 1101 14-15 0.37795368372563587 1101 16-17 0.3035972323459788 1101 18-19 0.23976031487695337 1101 20-21 0.3138819781982747 1101 22-23 0.25713601993412283 1101 24-25 0.34559824771933734 1101 26-27 0.3745851441659127 1101 28-29 0.2752662242509061 1101 30-31 0.3134936842020437 1101 32-33 0.4154871895400589 1101 34-35 0.3641526204974852 1101 36-37 0.33766180609050167 1101 38-39 0.3387185127588026 1101 40-41 0.34584294669856774 1101 42-43 0.386013204398445 1101 44-45 0.3909643393450253 1101 46-47 0.3612974675635954 1101 48-49 0.35684376592433154 1101 50-51 0.3337203817776029 1101 52-53 0.3527709691339851 1101 54-55 0.4156319969668303 1101 56-57 0.4107508072244599 1101 58-59 0.35433200852178004 1101 60-61 0.41049519301878945 1101 62-63 0.42896375850808965 1101 64-65 0.35581765768596085 1101 66-67 0.4178338876565846 1101 68-69 0.33490110591803557 1101 70-71 0.32447769203108123 1101 72-73 0.4161738423857031 1101 74-75 0.331063740135221 1101 76-77 0.2903747206851719 1101 78-79 0.35337637037705605 1101 80-81 0.2689256029481193 1101 82-83 0.2982460113706793 1101 84-85 0.2464793824085696 1101 86-87 0.3062230538292603 1101 88-89 0.38305119561978174 1101 90-91 0.3784310557032313 1101 92-93 0.4039955932020298 1101 94-95 0.4197148731100242 1101 96-97 0.35606856770720796 1101 98-99 0.3347722877857926 1102 1 0.16120232656236766 1102 2 0.16229084523819282 1102 3 0.18359091231796398 1102 4 0.20041174822758023 1102 5 0.1274980738823146 1102 6 0.12999706213270912 1102 7 0.018534735221429344 1102 8 0.12417744903545724 1102 9 0.09631786676406051 1102 10-11 0.12065871575995146 1102 12-13 0.12641158583621603 1102 14-15 0.1802661662631735 1102 16-17 0.22870496167184484 1102 18-19 0.17708996684396539 1102 20-21 0.24921743164237853 1102 22-23 0.19395796032496548 1102 24-25 0.20318066579871186 1102 26-27 0.2735940046256573 1102 28-29 0.11145020801246375 1102 30-31 0.16568144277894703 1102 32-33 0.2758608250282606 1102 34-35 0.2036897496309109 1102 36-37 0.3056754982872505 1102 38-39 0.24387521219051678 1102 40-41 0.20115433288946605 1102 42-43 0.21180644896627854 1102 44-45 0.16403436427705742 1102 46-47 0.29632133980292963 1102 48-49 0.23267576134622914 1102 50-51 0.2705253399653955 1102 52-53 0.1795389874648592 1102 54-55 0.23467258750014253 1102 56-57 0.2811869421925195 1102 58-59 0.25070225214798825 1102 60-61 0.2568097317352027 1102 62-63 0.27522838737419164 1102 64-65 0.1490592747380859 1102 66-67 0.16540528484913608 1102 68-69 0.17339449102625792 1102 70-71 0.2362594115752188 1102 72-73 0.21662080646366633 1102 74-75 0.24352465866768824 1102 76-77 0.1986268112999241 1102 78-79 0.21662794040752686 1102 80-81 0.15730427046010576 1102 82-83 0.15395926591541453 1102 84-85 0.0329354553086425 1102 86-87 0.21493250988676493 1102 88-89 0.18577035031664835 1102 90-91 0.10631780433726945 1102 92-93 0.2905466613558616 1102 94-95 0.21869008535167467 1102 96-97 0.1413776804408613 1102 98-99 0.12193964290252524 1103 1 0.13339962079716017 1103 2 0.215842889170073 1103 3 0.2062515547972339 1103 4 0.24664485558319882 1103 5 0.12605158069041522 1103 6 0.19412298035285147 1103 7 0.08717803628555032 1103 8 0.1250492517444428 1103 9 0.20733236142198308 1103 10-11 0.16986356909564648 1103 12-13 0.158858354176175 1103 14-15 0.16133282040740227 1103 16-17 0.1309963202846376 1103 18-19 0.2238068217662601 1103 20-21 0.24672816730949165 1103 22-23 0.1657339621008731 1103 24-25 0.22352660684919812 1103 26-27 0.3189016647792826 1103 28-29 0.24016709891643728 1103 30-31 0.2876107367775944 1103 32-33 0.31367688920087744 1103 34-35 0.26747873460308824 1103 36-37 0.28954609939840026 1103 38-39 0.31602792850306116 1103 40-41 0.3011286143953811 1103 42-43 0.23334975579910378 1103 44-45 0.20226729943066601 1103 46-47 0.2123680039235296 1103 48-49 0.25228441980303273 1103 50-51 0.288336734650791 1103 52-53 0.2452828986254758 1103 54-55 0.3011101763345394 1103 56-57 0.2782551465983758 1103 58-59 0.18886649315122384 1103 60-61 0.29301395237912686 1103 62-63 0.2927187744482964 1103 64-65 0.273608564666759 1103 66-67 0.2743014711632128 1103 68-69 0.24982198641666997 1103 70-71 0.24566562298748806 1103 72-73 0.2790241917430336 1103 74-75 0.3001285018162676 1103 76-77 0.23285572629982454 1103 78-79 0.252936128153884 1103 80-81 0.2835043794530101 1103 82-83 0.24624692129791015 1103 84-85 0.26836197541436135 1103 86-87 0.4005528418029023 1103 88-89 0.3885058997464448 1103 90-91 0.3610659841691337 1103 92-93 0.39320745038610383 1103 94-95 0.38388824383986275 1103 96-97 0.15185401424712452 1103 98-99 0.3269809075808503 1104 1 0.10325798648446494 1104 2 0.1026320196426802 1104 3 0.04475149056440486 1104 4 0.10611822598522735 1104 5 0.05985338483692715 1104 6 0.02056159209446662 1104 7 -0.038676760129696675 1104 8 -0.014541300069240037 1104 9 -0.037015020081405225 1104 10-11 0.01416336369867821 1104 12-13 -0.029912821832517977 1104 14-15 0.050783781511917425 1104 16-17 0.08049542454210012 1104 18-19 0.07657472449221103 1104 20-21 0.09370219022080306 1104 22-23 0.101679814566765 1104 24-25 0.0957961377940606 1104 26-27 0.07811726162072574 1104 28-29 -0.02071047608881571 1104 30-31 0.011119313433646028 1104 32-33 0.10314304312790767 1104 34-35 0.11658171482123691 1104 36-37 0.16850243903049744 1104 38-39 0.05903675894356297 1104 40-41 0.04464493971484984 1104 42-43 -0.04407318701961316 1104 44-45 -0.02733022543966257 1104 46-47 -0.006472618509235417 1104 48-49 -0.052818517469816584 1104 50-51 0.04072166533301669 1104 52-53 -0.023434607800581375 1104 54-55 0.11689355676578117 1104 56-57 0.012976715797123006 1104 58-59 0.07026723436474924 1104 60-61 0.10611316414791361 1104 62-63 0.06760861607272517 1104 64-65 0.01085442387061164 1104 66-67 0.027247184982449824 1104 68-69 -0.04666799030031399 1104 70-71 -0.007643089612628273 1104 72-73 -0.01510102191905105 1104 74-75 -0.11632733339376244 1104 76-77 -0.12087505799596698 1104 78-79 -0.12311104743423584 1104 80-81 -0.09712826578075351 1104 82-83 0.023186405643421892 1104 84-85 -0.07050505929839446 1104 86-87 0.0037036980616811377 1104 88-89 0.046668230913137876 1104 90-91 -0.015930430454332623 1104 92-93 0.004690299480419213 1104 94-95 -0.09000734023646828 1104 96-97 -0.26594408346155873 1104 98-99 0.04101934861492751 1105 1 0.040449622308447175 1105 2 0.04891739399577233 1105 3 0.033642111100956384 1105 4 -0.005742149715494804 1105 5 0.0059930135366315085 1105 6 -0.015482239411902299 1105 7 -0.051780383474088865 1105 8 -0.14760307539761186 1105 9 -0.1286632019185845 1105 10-11 -0.1455731435981349 1105 12-13 -0.07737196299475357 1105 14-15 -0.12002769486276321 1105 16-17 -0.0751308178896295 1105 18-19 -0.09393816031096236 1105 20-21 -0.03764595982332963 1105 22-23 -0.055793473036402474 1105 24-25 -0.008249032907485798 1105 26-27 -0.006617306740018591 1105 28-29 -0.008473071289195389 1105 30-31 0.020659438134288166 1105 32-33 -0.017577211575869 1105 34-35 -0.04932425328817658 1105 36-37 -0.08656799797910963 1105 38-39 -0.16322557333158727 1105 40-41 -0.21976731421445095 1105 42-43 -0.08221186247782697 1105 44-45 -0.13302950571392813 1105 46-47 -0.1994622828340198 1105 48-49 -0.19835757103432172 1105 50-51 -0.11745663409445228 1105 52-53 -0.15363281290500197 1105 54-55 -0.03444969505829221 1105 56-57 -0.049806405539754905 1105 58-59 -0.14054415858525715 1105 60-61 -0.06852757804198006 1105 62-63 -0.026615783828852102 1105 64-65 -0.19311923080111626 1105 66-67 -0.12663226398200322 1105 68-69 -0.19500656044176878 1105 70-71 -0.2651308848580456 1105 72-73 -0.1547959663105516 1105 74-75 -0.1764603187392524 1105 76-77 -0.1927209865602464 1105 78-79 -0.10755356985163189 1105 80-81 -0.15925836903299384 1105 82-83 -0.10440201900906487 1105 84-85 -0.20897150662845476 1105 86-87 -0.10555077964302839 1105 88-89 -0.1434302917917556 1105 90-91 -0.06130193335622636 1105 92-93 -0.015986386458873625 1105 94-95 -0.11463574290884537 1105 96-97 -0.20172384837633217 1105 98-99 -0.14198517080211914 1106 1 0.050394860771422856 1106 2 0.09535212471523025 1106 3 0.038096051699859856 1106 4 0.06673687281804064 1106 5 -0.0828519982141529 1106 6 0.00407363340583089 1106 7 0.004207421329525118 1106 8 -0.03322815724682471 1106 9 0.0075845300830721385 1106 10-11 -0.03212221060122289 1106 12-13 -0.028548810266137536 1106 14-15 -0.04370886217970593 1106 16-17 0.012039887578978892 1106 18-19 0.03551373155516302 1106 20-21 0.015785765002526375 1106 22-23 -0.0012636763826350261 1106 24-25 0.028033259280761058 1106 26-27 0.1174327997558251 1106 28-29 -0.04230519475351713 1106 30-31 -0.028954191774765548 1106 32-33 0.05275574002138228 1106 34-35 -0.09812138866770681 1106 36-37 -0.032950908438870385 1106 38-39 -0.04665684721378227 1106 40-41 0.01673359666860108 1106 42-43 0.044812817148859097 1106 44-45 0.05115167732554937 1106 46-47 0.0056137722660238865 1106 48-49 -0.0559747452466155 1106 50-51 -0.0619950088212633 1106 52-53 -0.07950159082793107 1106 54-55 -0.04097987617089416 1106 56-57 0.01837907370791214 1106 58-59 0.03009811740389523 1106 60-61 0.02428916356337396 1106 62-63 0.0288982543016445 1106 64-65 -0.0848473607856377 1106 66-67 -0.11923953300511414 1106 68-69 -0.0671837692688797 1106 70-71 -0.026344144317050677 1106 72-73 0.04503304614081571 1106 74-75 -0.0838966100903562 1106 76-77 -0.1200436707185446 1106 78-79 -0.03389732455693206 1106 80-81 -0.08836933219045662 1106 82-83 -0.043333610518246246 1106 84-85 -0.06481615550662312 1106 86-87 -0.16469442482632246 1106 88-89 -0.0947761495067887 1106 90-91 -0.13087461156630198 1106 92-93 -0.11554369023080469 1106 94-95 -0.1411023584056892 1106 96-97 -0.2245500726113967 1106 98-99 0.1651908189288953 1107 1 0.08192419167180276 1107 2 0.15352271360118763 1107 3 0.05243081742626643 1107 4 0.09309110391258457 1107 5 0.16584677289023375 1107 6 0.05249956317548765 1107 7 0.033036544642129684 1107 8 0.07111322347427773 1107 9 -0.033899675953520614 1107 10-11 0.053948717528392365 1107 12-13 0.01939863546780174 1107 14-15 0.06334724239168565 1107 16-17 0.06413794005874252 1107 18-19 -0.019595318385910332 1107 20-21 0.05026661410989419 1107 22-23 -0.009238034640688397 1107 24-25 0.13147191210197917 1107 26-27 0.15598221435795523 1107 28-29 0.06395783713848857 1107 30-31 0.07591231126194486 1107 32-33 -0.021933150277362756 1107 34-35 -0.06256294639496218 1107 36-37 0.05414304197834241 1107 38-39 0.0412000598597686 1107 40-41 -0.036226189193897085 1107 42-43 -0.026819516555299572 1107 44-45 0.017261948181776177 1107 46-47 -0.09569899653612879 1107 48-49 -0.06350935561071935 1107 50-51 0.027770960829819558 1107 52-53 -0.0444854849998606 1107 54-55 5.315217495436286E-4 1107 56-57 0.03202589652187271 1107 58-59 0.09435970567621865 1107 60-61 0.1722490847543412 1107 62-63 -0.11154496687789361 1107 64-65 -0.05392274796647456 1107 66-67 0.11887841876416871 1107 68-69 -0.008312265954110387 1107 70-71 0.08700395940041972 1107 72-73 0.049180567745722215 1107 74-75 -0.01567943166016761 1107 76-77 0.022624920484268785 1107 78-79 0.07044075780474657 1107 80-81 -0.102932459264931 1107 82-83 -0.05194739795647507 1107 84-85 -0.08333859318394587 1107 86-87 -0.0722414453764948 1107 88-89 -0.02420201478873807 1107 90-91 0.021499271658370844 1107 92-93 0.004402024343097111 1107 94-95 0.007386392491682159 1107 96-97 -0.0546243527096415 1107 98-99 0.30006968602685546 1108 1 0.022126195209629884 1108 2 0.059923116238067564 1108 3 0.05431914850657549 1108 4 -0.008280833114739039 1108 5 -0.02693799041347944 1108 6 -0.1380454591328757 1108 7 0.007561271511455914 1108 8 -0.12582469474015312 1108 9 -0.03204993982373594 1108 10-11 -0.05566965667075152 1108 12-13 -0.054729938782266174 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0.26031379639399077 2215 82-83 0.14393768660152517 2215 84-85 0.1636878873454144 2215 86-87 0.11966544707248161 2215 88-89 -0.03187303936286767 2215 90-91 0.2129994024536188 2215 92-93 0.2548164811476177 2215 94-95 0.20026605506225792 2215 96-97 0.18392783337659324 2215 98-99 0.17358505853605877 2216 1 -0.06490770191243911 2216 2 -0.033879574527873046 2216 3 -0.005245161744387161 2216 4 -0.0022861527948734306 2216 5 -0.031876144031329545 2216 6 0.056184932197574255 2216 7 0.05262091657260015 2216 8 0.045413059740610606 2216 9 0.053191867235312884 2216 10-11 0.059179488411338355 2216 12-13 0.08700640953758665 2216 14-15 -0.0071909768963607235 2216 16-17 0.004331543898452139 2216 18-19 -0.09803220076499741 2216 20-21 -0.08063887255956814 2216 22-23 -0.15203584370092216 2216 24-25 -0.056753129061561935 2216 26-27 -0.19691900503404014 2216 28-29 -0.058821987682982524 2216 30-31 -0.09067002751753961 2216 32-33 -0.07021519330836412 2216 34-35 -0.12935195625694718 2216 36-37 -0.17933132578303201 2216 38-39 -0.06786807735433342 2216 40-41 -0.1061713389916008 2216 42-43 -0.11039067089489407 2216 44-45 -0.049535461228707334 2216 46-47 0.03903952245087794 2216 48-49 0.10333453897226264 2216 50-51 0.02916961989368616 2216 52-53 0.12004548459569264 2216 54-55 0.10318225049666552 2216 56-57 0.17541980504657317 2216 58-59 0.05174250608568798 2216 60-61 0.029654686677872633 2216 62-63 0.07344308390140242 2216 64-65 0.1575904249418727 2216 66-67 0.09304382390047294 2216 68-69 0.013147161350097747 2216 70-71 0.02519776114729666 2216 72-73 0.0016497089935256781 2216 74-75 0.10642106529394368 2216 76-77 0.03918503306123711 2216 78-79 0.11141843456634604 2216 80-81 0.20404844767920238 2216 82-83 0.13023915409790732 2216 84-85 0.1765216329595134 2216 86-87 0.1296614167217136 2216 88-89 0.15731918946871914 2216 90-91 0.33215273735427076 2216 92-93 0.36787127566717714 2216 94-95 0.3383604371197464 2216 96-97 0.36200997505608257 2216 98-99 0.35684639572810184 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 5.0 7 228.0 8 1300.0 9 2309.0 10 3240.0 11 5417.0 12 9455.0 13 15821.0 14 23320.0 15 27547.0 16 29043.0 17 31358.0 18 32668.0 19 34840.0 20 38042.0 21 41718.0 22 46622.0 23 53231.0 24 61174.0 25 69958.0 26 79122.0 27 90094.0 28 107707.0 29 135184.0 30 171847.0 31 222682.0 32 297575.0 33 406459.0 34 562925.0 35 828720.0 36 1309447.0 37 1652536.0 38 499694.0 39 418.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.13125708839702 18.329332294765877 12.454117683932656 25.08529293290444 2 16.322765364628147 20.73592518311141 37.265692993868285 25.67561645839216 3 18.37157355751037 25.566681989455137 28.724801558281154 27.336942894753335 4 13.266294296361453 17.07313109410065 35.807055611484294 33.8535189980536 5 14.80790097546239 37.06433501371068 32.96051804879663 15.167245962030302 6 32.63585242899219 35.95330386989811 17.306774258797457 14.104069442312253 7 28.54261339645075 31.26224479105754 21.766439253212486 18.428702559279227 8 27.174592183706036 32.85601562225667 20.039856604445983 19.929535589591314 9 27.084745054417585 15.471669859393305 19.01246222633409 38.43112285985502 10-11 25.832341077811506 25.291357466496684 26.57897043199463 22.297331023697183 12-13 26.418661794336558 23.18124133559963 26.18333544698512 24.216761423078697 14-15 24.476109398746843 24.39325618359228 23.313226072034993 27.81740834562589 16-17 24.564469233017196 26.59422790235103 24.28459658609929 24.556706278532488 18-19 25.140081425411935 25.95663541073865 25.191084761886245 23.71219840196317 20-21 26.466169439308224 25.356288151965202 24.169016479719104 24.008525929007472 22-23 25.41882227709656 25.10776722048213 24.267815545236548 25.20559495718477 24-25 24.914409545761682 25.738197942114322 24.205249634925067 25.142142877198932 26-27 25.21841111887407 25.60791560288782 24.108199640002002 25.065473638236114 28-29 24.81293987897433 25.337689193307313 24.847107138192744 25.00226378952562 30-31 25.14384031736865 25.51898161113344 24.50707158061834 24.83010649087957 32-33 24.776303509262732 25.644746205002424 24.069381099362364 25.50956918637248 34-35 25.345832618101106 25.53157776287741 24.57174941280825 24.55084020621324 36-37 25.194277004851916 25.651739932028438 24.08208504541546 25.071898017704182 38-39 25.1933882834509 25.323015131959366 24.25827135665267 25.225325227937063 40-41 25.00755981175053 25.68032501676653 24.595796744666707 24.716318426816233 42-43 25.660772528601772 25.430931035073172 24.342666387683977 24.565630048641076 44-45 25.003424406090453 25.773719888805473 24.01558482036233 25.207270884741746 46-47 24.539809156107356 25.65511355243535 24.859875044002166 24.94520224745513 48-49 25.16931971462467 25.855369998908834 24.38612353185631 24.589186754610186 50-51 24.551402802150875 26.10077243573652 24.3051575328373 25.042667229275306 52-53 24.795145062775457 25.544669200920644 24.36417775221404 25.29600798408986 54-55 24.986164528782858 25.429762964351642 24.611054215023103 24.973018291842397 56-57 24.941052331599753 25.121334253086246 24.3399892566514 25.5976241586626 58-59 24.728202276765725 25.244192076678836 24.656710544529904 25.370895102025536 60-61 24.8526634769388 25.38640650079966 24.51382865142535 25.24710137083619 62-63 24.375568255523376 25.60443669535526 24.439993522648816 25.580001526472547 64-65 24.630447091039578 25.17698085205608 24.495589335935108 25.696982720969235 66-67 25.02894240879632 25.381344394749426 24.324298049096278 25.265415147357977 68-69 25.089012793058785 25.085675448140126 24.396738630463926 25.428573128337163 70-71 24.436881085757285 25.00863356620262 24.705174596826968 25.84931075121312 72-73 25.005992710658294 25.34755545288786 24.513879437108894 25.132572399344948 74-75 25.314704370732006 25.137324488305218 24.480331865578712 25.067639275384064 76-77 25.022200598806737 25.255386692351646 24.637767484567682 25.08464522427393 78-79 25.10728112893963 25.248051788628246 24.63795611710656 25.006710965325567 80-81 24.787628781610824 25.009242994405156 25.099699551896148 25.10342867208787 82-83 25.03288010254645 25.187827223041726 24.500522802336604 25.27876987207522 84-85 24.884832579915624 25.24268301636779 24.36535307803322 25.507131325683364 86-87 24.827959869443067 24.918829967500063 24.579966121596016 25.67324404146085 88-89 24.925874667317498 24.554957799999013 24.806949106650805 25.712218426032685 90-91 25.25430568280191 24.844653849134016 24.509149113441577 25.391891354622498 92-93 24.942220402321283 24.910602686765802 24.63844946374671 25.5087274471662 94-95 24.416624853120545 25.10317474367014 24.952595191959727 25.527605211249583 96-97 25.085022489351694 25.03842299715049 24.42771789742627 25.448836616071553 98-99 24.889432311825257 25.05718467967148 24.41838058675167 25.635002421751597 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 987.0 1 894.0 2 875.0 3 893.5 4 861.5 5 814.0 6 693.5 7 627.5 8 612.0 9 621.0 10 581.0 11 540.0 12 558.5 13 559.0 14 621.0 15 771.0 16 894.5 17 1042.5 18 1386.0 19 1888.5 20 2555.5 21 3523.5 22 4800.5 23 6565.0 24 8684.0 25 11678.5 26 16125.5 27 20498.5 28 25440.5 29 31774.0 30 37652.0 31 44184.0 32 51464.5 33 59323.0 34 67555.0 35 75168.0 36 83064.5 37 92380.0 38 104132.0 39 112405.5 40 120978.5 41 130057.0 42 140728.0 43 164031.5 44 187948.0 45 212443.0 46 253031.5 47 286856.5 48 305582.5 49 328274.0 50 355262.5 51 353351.5 52 341363.5 53 339400.0 54 321216.0 55 303590.0 56 279230.5 57 261641.0 58 248990.5 59 219195.5 60 182827.5 61 140809.0 62 114300.0 63 92657.0 64 68827.0 65 53709.0 66 39293.5 67 28960.0 68 22966.5 69 19675.5 70 17791.5 71 16263.5 72 15945.5 73 14229.0 74 10363.0 75 7681.5 76 5549.0 77 3725.5 78 2345.0 79 1541.5 80 1098.5 81 756.5 82 566.0 83 425.0 84 276.0 85 190.5 86 145.0 87 109.0 88 69.0 89 52.0 90 52.0 91 36.0 92 24.5 93 15.5 94 10.0 95 9.0 96 6.5 97 5.0 98 4.5 99 12.0 100 21.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0023506516383606612 2 0.0 3 1.4510195298522602E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 3.3373449186601984E-4 22-23 0.0 24-25 0.004534436030788313 26-27 1.4510195298522602E-5 28-29 0.008793178350904696 30-31 5.223670307468137E-4 32-33 2.9020390597045203E-5 34-35 7.255097649261301E-5 36-37 0.0 38-39 1.4510195298522602E-5 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 1.4510195298522602E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 3.6275488246306504E-5 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 6891706.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.992439122221725 #Duplication Level Percentage of deduplicated Percentage of total 1 73.92852731369216 28.087250733609554 2 13.598938820280143 10.333137105126221 3 4.730886669501377 5.39213771355484 4 2.2797283285196537 3.4644975894594894 5 1.2608666188190185 2.395169912836155 6 0.8003830434810862 1.824510243234823 7 0.5705735787684113 1.5174237369274932 8 0.38486350328686975 1.1697522575193102 9 0.3087021117719832 1.0555511569558524 >10 1.7701957020844064 12.558830649357317 >50 0.17913320714881226 4.775113861435526 >100 0.15425785255207625 12.00191291600254 >500 0.01953615191472747 5.143708438096821 >1k 0.01298573223695127 9.1895437145254 >5k 3.447539531723507E-4 0.727785514723239 >10k+ 7.661198959385572E-5 0.3636744566353187 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 13155 0.1908816191520648 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 11834 0.17171365116271647 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.4510195298522602E-5 2 0.0 0.0 0.0 0.0 1.4510195298522602E-5 3 0.0 0.0 0.0 0.0 1.4510195298522602E-5 4 0.0 1.4510195298522602E-5 0.0 0.0 1.4510195298522602E-5 5 0.0 1.4510195298522602E-5 0.0 1.4510195298522602E-5 1.4510195298522602E-5 6 0.0 1.4510195298522602E-5 0.0 1.4510195298522602E-5 1.4510195298522602E-5 7 0.0 1.4510195298522602E-5 0.0 1.4510195298522602E-5 1.4510195298522602E-5 8 1.4510195298522602E-5 1.4510195298522602E-5 0.0 1.4510195298522602E-5 1.4510195298522602E-5 9 1.4510195298522602E-5 7.255097649261301E-5 0.0 2.9020390597045203E-5 2.9020390597045203E-5 10-11 2.9020390597045203E-5 7.255097649261301E-5 0.0 5.0785683544829106E-5 2.9020390597045203E-5 12-13 2.9020390597045203E-5 7.255097649261301E-5 0.0 6.529587884335171E-5 2.9020390597045203E-5 14-15 4.3530585895567805E-5 1.2333666003744211E-4 0.0 8.706117179113561E-5 2.9020390597045203E-5 16-17 5.8040781194090407E-5 1.3059175768670341E-4 0.0 1.4510195298522602E-4 2.9020390597045203E-5 18-19 7.255097649261301E-5 1.3059175768670341E-4 0.0 1.7412234358227122E-4 2.9020390597045203E-5 20-21 7.255097649261301E-5 1.3059175768670341E-4 0.0 3.6275488246306504E-4 2.9020390597045203E-5 22-23 7.255097649261301E-5 1.3784685533596472E-4 0.0 0.0012406216980236824 2.9020390597045203E-5 24-25 7.255097649261301E-5 1.5961214828374862E-4 0.0 0.0032793041374661078 2.9020390597045203E-5 26-27 7.255097649261301E-5 1.5961214828374862E-4 0.0 0.005702506752319382 2.9020390597045203E-5 28-29 7.255097649261301E-5 1.5961214828374862E-4 0.0 0.015750816996546284 2.9020390597045203E-5 30-31 1.0157136708965821E-4 2.0314273417931642E-4 0.0 0.04319685140370178 2.9020390597045203E-5 32-33 1.1608156238818081E-4 2.0314273417931642E-4 0.0 0.0928362295199476 2.9020390597045203E-5 34-35 1.3059175768670341E-4 2.1765292947783902E-4 0.0 0.15692050705587268 2.9020390597045203E-5 36-37 1.3059175768670341E-4 2.3216312477636163E-4 0.0 0.2523975921201514 2.9020390597045203E-5 38-39 1.4510195298522602E-4 2.4667332007488423E-4 0.0 0.39437259801854574 2.9020390597045203E-5 40-41 1.4510195298522602E-4 2.7569371067192943E-4 0.0 0.5609496400455852 2.9020390597045203E-5 42-43 1.4510195298522602E-4 2.9020390597045203E-4 0.0 0.7155485158536943 2.9020390597045203E-5 44-45 1.4510195298522602E-4 3.4824468716454244E-4 0.0 0.899791720656685 2.9020390597045203E-5 46-47 1.4510195298522602E-4 3.9177527306011024E-4 0.0 1.1035148626479423 2.9020390597045203E-5 48-49 1.4510195298522602E-4 3.9903037070937155E-4 0.0 1.3163939378725673 2.9020390597045203E-5 50-51 1.4510195298522602E-4 5.006017377990298E-4 0.0 1.5466562270648225 2.9020390597045203E-5 52-53 1.4510195298522602E-4 5.078568354482911E-4 0.0 1.7825774924235014 3.6275488246306504E-5 54-55 1.4510195298522602E-4 5.151119330975523E-4 0.0 2.0213572662559893 5.8040781194090407E-5 56-57 1.4510195298522602E-4 5.223670307468137E-4 0.0 2.289164685783172 7.255097649261301E-5 58-59 1.4510195298522602E-4 5.368772260453363E-4 0.0 2.5806019583539985 7.255097649261301E-5 60-61 1.4510195298522602E-4 5.441323236945975E-4 0.0 2.878104492559607 8.706117179113561E-5 62-63 1.4510195298522602E-4 5.513874213438589E-4 0.0 3.1815054211540654 8.706117179113561E-5 64-65 1.4510195298522602E-4 6.094282025379493E-4 0.0 3.501462482584138 8.706117179113561E-5 66-67 1.4510195298522602E-4 6.239383978364719E-4 0.0 3.8475088751609547 9.431626944039691E-5 68-69 1.4510195298522602E-4 6.384485931349945E-4 0.0 4.2288948483873225 1.0157136708965821E-4 70-71 1.4510195298522602E-4 6.384485931349945E-4 0.0 4.652701377568921 1.0157136708965821E-4 72-73 1.4510195298522602E-4 6.384485931349945E-4 0.0 5.065226229905919 1.0157136708965821E-4 74-75 1.5235705063448732E-4 6.529587884335171E-4 0.0 5.482125906125421 1.0157136708965821E-4 76-77 1.5961214828374862E-4 6.74724081381301E-4 0.0 5.926928687903982 1.0157136708965821E-4 78-79 1.5961214828374862E-4 6.819791790305623E-4 0.0 6.397225882822047 1.0157136708965821E-4 80-81 1.5961214828374862E-4 6.819791790305623E-4 0.0 6.902267740382425 1.1608156238818081E-4 82-83 1.5961214828374862E-4 6.892342766798235E-4 0.0 7.433369908698949 1.3059175768670341E-4 84-85 1.5961214828374862E-4 7.109995696276075E-4 0.0 7.956992071339085 1.3059175768670341E-4 86-87 1.5961214828374862E-4 7.109995696276075E-4 0.0 8.503518577257939 1.3059175768670341E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGACCG 945 0.0 22.142508 5 GTACTAG 1870 0.0 21.882483 1 GTTATAT 1900 0.0 21.292233 1 TATTCGC 385 0.0 20.532143 9 ATGGGTA 3670 0.0 20.272158 5 TATATAC 3370 0.0 19.179222 3 GTATAAG 2560 0.0 18.527452 1 TAGGCAT 3020 0.0 18.322557 5 TATACTT 2975 0.0 18.287106 5 GTATCAA 29455 0.0 18.233854 1 CATGGGG 14915 0.0 18.17571 4 ATATACT 2540 0.0 18.12373 4 GTACAAG 2895 0.0 17.668499 1 GTACATG 44710 0.0 17.659912 1 TACATGG 43860 0.0 17.090017 2 TAGTACT 2565 0.0 17.040665 4 GTATATG 2620 0.0 17.038267 1 GTATTAT 1585 0.0 17.015873 1 CGTTAAC 520 0.0 16.990486 1 GTAATAC 1135 0.0 16.79709 3 >>END_MODULE