FastQCFastQC Report
Fri 27 May 2016
ERR765537_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765537_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6891706
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158660.2302187586063596No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT130440.1892709874739288No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA127820.1854693163057159No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97780.14188068962895398No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83870.12169700796870907No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG6100.030.492782
GTATCAA349200.027.6069721
GTTATAT16100.025.4308031
TAGACCG10050.024.5231745
CAACGCA426700.022.4715425
ATCAACG428500.022.3445913
AACGCAG445400.021.7460696
TCAACGC441550.021.7263224
AGTACTT226100.021.60712612-13
GTACTAG17400.021.3916361
ATATACT23850.021.2522534
TCTAACG8650.020.4284022
TATCAAC478250.020.311892
ACGCAGA477850.020.1330977
CTAACGC8950.019.743653
CGCAGAG488950.019.695068
TATATAC31900.019.6793163
TACTTTT262200.018.70331414-15
TATACTT30000.018.4455915
GCAGAGT520150.018.4068249
Produced by FastQC (version 0.11.5)