FastQCFastQC Report
Fri 27 May 2016
ERR765536_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765536_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10107974
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA385080.3809665517540904No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT301670.29844754250456124No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG268870.2659979141220585No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG192920.19085921669367176No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT152690.1510589560281813No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142840.14131417433404558No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG129050.12767147996225553No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT121040.11974704327494314No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC112480.11127848172146071No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC103770.10266152247720463No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA101100.10002004358143383No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACAC86100.024.2892023
CTACACT93550.023.3112164
TACACTC79600.022.7355275
ATCTACA85700.020.509232
GTATAAG41500.020.045061
GTACTAG29100.019.7037561
CATGGGG216850.019.7013174
GTATTAG31950.016.7693461
CACTCTT110150.015.9606647
GTGATCG23900.015.9312918
CGTTAAC9250.015.7507821
ACACTCT119150.015.3468556
TAGTACT39150.015.2462384
TCTAACG12650.015.2318222
ACATGGG717150.015.1966253
GTATATA32500.015.1840681
ATGGGGG131600.015.1092145
GTATAAA80050.015.0887751
GTACATG830850.014.9505351
TAGGCAT45300.014.9424475
Produced by FastQC (version 0.11.5)