##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765533_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9334178
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.50790707012444	34.0	31.0	34.0	31.0	34.0
2	32.63740256506786	34.0	31.0	34.0	31.0	34.0
3	32.68333462250238	34.0	31.0	34.0	31.0	34.0
4	36.18584807360648	37.0	35.0	37.0	35.0	37.0
5	36.124642362723314	37.0	35.0	37.0	35.0	37.0
6	36.18267511075962	37.0	37.0	37.0	35.0	37.0
7	36.13438848069964	37.0	36.0	37.0	35.0	37.0
8	36.14746976112947	37.0	37.0	37.0	35.0	37.0
9	37.87663348609808	39.0	38.0	39.0	35.0	39.0
10-11	37.83124159406431	39.0	38.0	39.0	35.0	39.0
12-13	37.799047168374116	39.0	38.0	39.0	35.0	39.0
14-15	39.160595341121635	40.5	39.0	41.0	36.0	41.0
16-17	39.13816433541336	40.0	38.5	41.0	36.0	41.0
18-19	39.16860349138403	40.0	39.0	41.0	36.0	41.0
20-21	39.15759609469629	40.0	39.0	41.0	36.0	41.0
22-23	39.09109345247113	40.0	39.0	41.0	35.5	41.0
24-25	39.02690119044227	40.0	39.0	41.0	35.0	41.0
26-27	38.88360512302208	40.0	38.0	41.0	35.0	41.0
28-29	38.74696202493674	40.0	38.0	41.0	35.0	41.0
30-31	38.557119705666636	40.0	38.0	41.0	34.5	41.0
32-33	38.30063466756259	40.0	38.0	41.0	34.0	41.0
34-35	38.22159760613093	40.0	38.0	41.0	34.0	41.0
36-37	38.10005942676474	40.0	38.0	41.0	34.0	41.0
38-39	37.91900834760169	40.0	37.0	41.0	33.0	41.0
40-41	37.691520560246445	40.0	37.0	41.0	33.0	41.0
42-43	37.43314033651383	40.0	37.0	41.0	33.0	41.0
44-45	37.08483087637711	40.0	36.0	41.0	32.0	41.0
46-47	36.73968275513923	39.5	36.0	41.0	31.0	41.0
48-49	36.445795012694205	39.0	35.5	41.0	31.0	41.0
50-51	36.16887759157796	39.0	35.0	41.0	30.0	41.0
52-53	36.01718656961545	39.0	35.0	41.0	30.0	41.0
54-55	35.984253192943186	39.0	35.0	41.0	30.0	41.0
56-57	35.79823740237222	39.0	35.0	41.0	30.0	41.0
58-59	35.659109886269576	38.5	35.0	41.0	30.0	41.0
60-61	35.32335911100046	38.0	35.0	40.0	29.0	41.0
62-63	34.9866870976748	37.0	35.0	40.0	29.0	41.0
64-65	34.7615402770335	37.0	35.0	39.5	29.0	41.0
66-67	34.40980453768934	36.0	35.0	39.0	29.0	41.0
68-69	34.062605994871745	36.0	34.0	39.0	28.0	41.0
70-71	33.68775938277586	35.0	34.0	38.5	28.0	40.5
72-73	33.31141965580686	35.0	34.0	37.0	27.5	39.5
74-75	32.960152302645184	35.0	34.0	37.0	26.5	39.0
76-77	31.542022982634357	34.5	32.0	35.5	25.0	37.0
78-79	32.31874509999702	35.0	33.5	36.0	26.0	37.0
80-81	32.274452608467506	35.0	34.0	35.5	26.0	37.0
82-83	32.11227105375535	35.0	34.0	35.0	26.0	36.5
84-85	31.930417600778558	35.0	34.0	35.0	26.0	36.0
86-87	31.756928944359107	35.0	33.0	35.0	25.0	36.0
88-89	31.607714573259692	35.0	33.0	35.0	25.0	36.0
90-91	31.479593757479236	35.0	33.0	35.0	25.0	35.0
92-93	31.334558704580093	35.0	33.0	35.0	24.0	35.0
94-95	31.242671341814997	35.0	33.0	35.0	24.0	35.0
96-97	31.1261837946523	35.0	33.0	35.0	23.0	35.0
98-99	30.989645365665837	35.0	33.0	35.0	20.0	35.0
100	30.65846033791085	35.0	33.0	35.0	18.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.43286885876491255
1101	2	0.37707908091957165
1101	3	0.3715295554005422
1101	4	0.2639758162970125
1101	5	0.3029756870645173
1101	6	0.3166879645234246
1101	7	0.29034296623267863
1101	8	0.2860430311687878
1101	9	0.3644898334050666
1101	10-11	0.39738585073222765
1101	12-13	0.40158052010753664
1101	14-15	0.5359956967215638
1101	16-17	0.4862848059913816
1101	18-19	0.46992624184746745
1101	20-21	0.4693748088673715
1101	22-23	0.5183318761915388
1101	24-25	0.5130997484753834
1101	26-27	0.5488932735034453
1101	28-29	0.5648232539013875
1101	30-31	0.604799461087417
1101	32-33	0.6000123254036396
1101	34-35	0.5596083724960579
1101	36-37	0.6477386690516909
1101	38-39	0.6490316459434666
1101	40-41	0.6610251337362811
1101	42-43	0.6860512757883157
1101	44-45	0.7081432922368478
1101	46-47	0.7487651417028403
1101	48-49	0.7535460102205676
1101	50-51	0.8294992244213688
1101	52-53	0.8267522052394369
1101	54-55	0.9348328732892099
1101	56-57	0.9239949160640677
1101	58-59	0.9653565072215287
1101	60-61	1.0077563817630733
1101	62-63	1.0404667706624977
1101	64-65	0.9570497768064428
1101	66-67	1.007483938986205
1101	68-69	1.018974782792661
1101	70-71	1.0215692417463202
1101	72-73	0.9816590593582504
1101	74-75	1.0188586382019196
1101	76-77	1.0457787352699341
1101	78-79	0.9649899062963598
1101	80-81	0.9627325547039121
1101	82-83	0.964025726568174
1101	84-85	1.0045835503758305
1101	86-87	0.9908651772620551
1101	88-89	1.0347725569107844
1101	90-91	1.0038656589363875
1101	92-93	1.1055037366696219
1101	94-95	1.1058292607894735
1101	96-97	1.1579392892921625
1101	98-99	1.123622866108466
1101	100	1.1884050239190245
1102	1	0.38482694945488305
1102	2	0.32902358184322367
1102	3	0.3677340136085334
1102	4	0.2799817457449265
1102	5	0.27447422816313605
1102	6	0.2781960115071769
1102	7	0.28209627200415355
1102	8	0.2660044167688511
1102	9	0.34990551168559136
1102	10-11	0.3220919937584412
1102	12-13	0.3820423462454201
1102	14-15	0.45528798618010313
1102	16-17	0.5043563799191944
1102	18-19	0.46403542571422207
1102	20-21	0.5116446995410016
1102	22-23	0.5177814906557714
1102	24-25	0.5173639634230867
1102	26-27	0.5659905550288329
1102	28-29	0.6219150091535255
1102	30-31	0.6081572455731887
1102	32-33	0.6601793995895093
1102	34-35	0.707640862812049
1102	36-37	0.8071260347397882
1102	38-39	0.7917785339391159
1102	40-41	0.8396011278137792
1102	42-43	0.8550105985939211
1102	44-45	0.9290569694155906
1102	46-47	0.9481463555018479
1102	48-49	0.9383204496427098
1102	50-51	1.0069555746828271
1102	52-53	1.0518988860867253
1102	54-55	1.1589121666213629
1102	56-57	1.127416965199238
1102	58-59	1.090508627450994
1102	60-61	1.124060295511498
1102	62-63	1.0640525921221666
1102	64-65	1.121149298384104
1102	66-67	1.160820330885862
1102	68-69	1.1226381458601296
1102	70-71	1.0797591265808677
1102	72-73	1.0902676202159043
1102	74-75	1.1194135073178728
1102	76-77	1.1085616135546346
1102	78-79	1.0810530448372546
1102	80-81	1.0761811056212167
1102	82-83	1.0576854923445538
1102	84-85	1.088389241047036
1102	86-87	1.1086191468814555
1102	88-89	1.1008206111586283
1102	90-91	1.1401558091363206
1102	92-93	1.1806931679326844
1102	94-95	1.2175024703551394
1102	96-97	1.2311485942850986
1102	98-99	1.2442968455291812
1102	100	1.218990478698295
1103	1	0.391949914582959
1103	2	0.3020540694005689
1103	3	0.3305018453789188
1103	4	0.2449027866250475
1103	5	0.2807211327220642
1103	6	0.278670984104906
1103	7	0.2797392712328346
1103	8	0.27308110251561146
1103	9	0.3499120070373607
1103	10-11	0.37509649990872873
1103	12-13	0.3407278494321986
1103	14-15	0.46269349910991053
1103	16-17	0.44580606124099376
1103	18-19	0.42969896649285744
1103	20-21	0.425407943969212
1103	22-23	0.46734670804708145
1103	24-25	0.4635345475987833
1103	26-27	0.5068934089240145
1103	28-29	0.5104880615628886
1103	30-31	0.5098720184388554
1103	32-33	0.5932914850033839
1103	34-35	0.5628351144257309
1103	36-37	0.63003432849208
1103	38-39	0.6031458054854113
1103	40-41	0.6212659676627652
1103	42-43	0.6364748944571943
1103	44-45	0.6814143761463995
1103	46-47	0.72831929424256
1103	48-49	0.6382169299739715
1103	50-51	0.7502267962149176
1103	52-53	0.8144040620701603
1103	54-55	0.8716312832535209
1103	56-57	0.8100492065015601
1103	58-59	0.8182393139284869
1103	60-61	0.8776542548343613
1103	62-63	0.8642437990397127
1103	64-65	0.8655113330530497
1103	66-67	0.8556946864251813
1103	68-69	0.8707110561836728
1103	70-71	0.8152188756979086
1103	72-73	0.8261735188072166
1103	74-75	0.8726408852255574
1103	76-77	0.7994206238254122
1103	78-79	0.8271038709648053
1103	80-81	0.8042556397827099
1103	82-83	0.8405227431869235
1103	84-85	0.8457131561203148
1103	86-87	0.8748246435728895
1103	88-89	0.852945403104389
1103	90-91	0.8802934294018208
1103	92-93	0.9054039217994507
1103	94-95	0.9323106544983624
1103	96-97	0.951560237203136
1103	98-99	0.9329807248780249
1103	100	0.9923692794939747
1104	1	0.2853559229490372
1104	2	0.1949429019522242
1104	3	0.2586042664715791
1104	4	0.21164888879262378
1104	5	0.2474672348896334
1104	6	0.22647932483250344
1104	7	0.22813830786611788
1104	8	0.23679006436122307
1104	9	0.24228112859902495
1104	10-11	0.27299864213207314
1104	12-13	0.2713679812613279
1104	14-15	0.390803525729261
1104	16-17	0.4219969599607296
1104	18-19	0.33031374656638235
1104	20-21	0.3566729965741047
1104	22-23	0.35501181135548876
1104	24-25	0.3594769991135536
1104	26-27	0.3945103438967621
1104	28-29	0.4284637238774991
1104	30-31	0.4847078658212851
1104	32-33	0.48975618268369914
1104	34-35	0.4802910657503574
1104	36-37	0.5188478663295726
1104	38-39	0.5299654277442585
1104	40-41	0.5409668169465789
1104	42-43	0.5766916519676499
1104	44-45	0.5700821413891433
1104	46-47	0.5402929284167257
1104	48-49	0.5539958626650545
1104	50-51	0.5673734561211177
1104	52-53	0.5785655527533962
1104	54-55	0.5542247678523324
1104	56-57	0.4385978184929442
1104	58-59	0.49986055869968027
1104	60-61	0.5926460155137931
1104	62-63	0.6270515059734123
1104	64-65	0.5329039049886504
1104	66-67	0.5804874358230805
1104	68-69	0.5616287897367229
1104	70-71	0.5440717087565972
1104	72-73	0.5205086690163725
1104	74-75	0.5684679862523083
1104	76-77	0.5160970086650636
1104	78-79	0.5776983696338363
1104	80-81	0.6032948082451242
1104	82-83	0.6030832705034328
1104	84-85	0.5648828861241135
1104	86-87	0.5892409193999804
1104	88-89	0.60912869629745
1104	90-91	0.5743649213526361
1104	92-93	0.5737040865858596
1104	94-95	0.5811039109313718
1104	96-97	0.629979555240908
1104	98-99	0.6140125413313164
1104	100	0.6303233928163223
1105	1	0.1892388061127548
1105	2	0.1843277038671971
1105	3	0.1950277928722528
1105	4	0.16712545935492784
1105	5	0.20160286164616537
1105	6	0.17374104775464616
1105	7	0.19971368550491775
1105	8	0.15806255985351214
1105	9	0.18353088455092603
1105	10-11	0.17803812460244473
1105	12-13	0.22855799095762563
1105	14-15	0.2625667588784566
1105	16-17	0.30832370385871144
1105	18-19	0.2602489897367164
1105	20-21	0.31993626827880206
1105	22-23	0.3482767073130688
1105	24-25	0.32217161824265617
1105	26-27	0.3076663985044732
1105	28-29	0.31214746379360747
1105	30-31	0.2800671620439985
1105	32-33	0.28565620745766296
1105	34-35	0.3500965327558774
1105	36-37	0.33631104582919846
1105	38-39	0.28540629755628544
1105	40-41	0.2530912576867763
1105	42-43	0.235206198874927
1105	44-45	0.27024837300761106
1105	46-47	0.25370187427660795
1105	48-49	0.2154598231186
1105	50-51	0.16571940886028358
1105	52-53	0.18051265461045318
1105	54-55	0.22304209846654288
1105	56-57	0.24528389963011676
1105	58-59	0.1896600208915089
1105	60-61	0.2656142474242813
1105	62-63	0.2195389262168561
1105	64-65	0.21222660794232695
1105	66-67	0.2777075352142262
1105	68-69	0.2697157643770467
1105	70-71	0.2402155027160191
1105	72-73	0.2528689053369604
1105	74-75	0.2304829457854396
1105	76-77	0.17676928529337488
1105	78-79	0.2853969673621819
1105	80-81	0.2286515614456448
1105	82-83	0.2435661165882479
1105	84-85	0.2282133920542755
1105	86-87	0.21037730578185787
1105	88-89	0.28342307326521166
1105	90-91	0.32952431962436535
1105	92-93	0.2872219066087993
1105	94-95	0.33624522913563837
1105	96-97	0.3632609890460401
1105	98-99	0.36719793093051933
1105	100	0.41543355489707423
1106	1	0.19774805898362757
1106	2	0.12261948841415204
1106	3	0.16544645319660845
1106	4	0.12776402936007258
1106	5	0.1435560094661099
1106	6	0.10891350401931987
1106	7	0.16483126561438155
1106	8	0.15890909240887652
1106	9	0.18874646839904585
1106	10-11	0.18029476069737171
1106	12-13	0.1736971522708899
1106	14-15	0.24257086690739982
1106	16-17	0.21498477748021827
1106	18-19	0.18625908128943536
1106	20-21	0.26793123673757435
1106	22-23	0.30527144831509645
1106	24-25	0.2233958463025303
1106	26-27	0.26835438826885394
1106	28-29	0.25354632096242824
1106	30-31	0.2436962537071068
1106	32-33	0.3268197266210535
1106	34-35	0.2827439258590019
1106	36-37	0.31763297337455043
1106	38-39	0.2844604367223056
1106	40-41	0.35878090220970194
1106	42-43	0.3280007503235609
1106	44-45	0.39002966262199834
1106	46-47	0.38867619940118914
1106	48-49	0.46181373665461223
1106	50-51	0.35143536334131653
1106	52-53	0.41095942963972476
1106	54-55	0.41329267784586676
1106	56-57	0.4219981387463534
1106	58-59	0.37449263917893916
1106	60-61	0.3198271429083661
1106	62-63	0.270444736212454
1106	64-65	0.37374864855011225
1106	66-67	0.34429549520701386
1106	68-69	0.30382846036757627
1106	70-71	0.30315153250644045
1106	72-73	0.2891374000968696
1106	74-75	0.2873562909887184
1106	76-77	0.2260940112348777
1106	78-79	0.34520310554187716
1106	80-81	0.36592380456813345
1106	82-83	0.3803026175067856
1106	84-85	0.39997760903274227
1106	86-87	0.37309954577933624
1106	88-89	0.3958231195263586
1106	90-91	0.43885514773616663
1106	92-93	0.48659306170845795
1106	94-95	0.4728346499102791
1106	96-97	0.5413683656156252
1106	98-99	0.5158652317227315
1106	100	0.5330846812112036
1107	1	0.14854698318072934
1107	2	0.07438162707840235
1107	3	0.15927970998272656
1107	4	0.10716642258634579
1107	5	0.1683942884414904
1107	6	0.1412114446292705
1107	7	0.10910090618084212
1107	8	0.10089214207415154
1107	9	0.1225854170697076
1107	10-11	0.14832990698179316
1107	12-13	0.19276586029565834
1107	14-15	0.2035106296279281
1107	16-17	0.23215599052879554
1107	18-19	0.22891748602177842
1107	20-21	0.2450634457484071
1107	22-23	0.22958729731322336
1107	24-25	0.22752272237847393
1107	26-27	0.25288642819960927
1107	28-29	0.262840489062242
1107	30-31	0.3309941791342297
1107	32-33	0.3206376735793839
1107	34-35	0.25188471072875274
1107	36-37	0.28020878054682186
1107	38-39	0.265505657600805
1107	40-41	0.2862711100680926
1107	42-43	0.3025624785043135
1107	44-45	0.3194205232528802
1107	46-47	0.3480281621009098
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2311	30-31	-0.16732185788183784
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2312	60-61	-0.14229554247742016
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2312	64-65	-0.13954065266935345
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2312	78-79	-0.1833373327887884
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2312	82-83	-0.2450688408917543
2312	84-85	-0.2637749630952406
2312	86-87	-0.20869564357893822
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2312	90-91	-0.20144343474652615
2312	92-93	-0.20463973754214848
2312	94-95	-0.19668524730033354
2312	96-97	-0.22361887163527072
2312	98-99	-0.26763320545144254
2312	100	-0.28003606138771886
2313	1	-0.19078561669768135
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2313	28-29	-0.28505511468247846
2313	30-31	-0.2585139562876222
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2313	68-69	-0.2085991948211401
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2313	72-73	-0.1962231129852725
2313	74-75	-0.2553450794881442
2313	76-77	-0.23425506267487606
2313	78-79	-0.3193369933110617
2313	80-81	-0.352750031578708
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2313	84-85	-0.36032065655606615
2313	86-87	-0.33134935871481375
2313	88-89	-0.35996228245556594
2313	90-91	-0.40363166204629763
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2313	96-97	-0.5369439099322904
2313	98-99	-0.4469723674886552
2313	100	-0.4523998446175277
2314	1	-0.2886401745574716
2314	2	-0.24775656798868084
2314	3	-0.27225745382265387
2314	4	-0.1975728200326472
2314	5	-0.24855790549101897
2314	6	-0.2633265400722067
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2314	12-13	-0.3719597698316477
2314	14-15	-0.386008964279803
2314	16-17	-0.3763632569346811
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2314	20-21	-0.3949589967678051
2314	22-23	-0.3903299105626772
2314	24-25	-0.44601612838735605
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2314	52-53	-0.4220697823205839
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2314	78-79	-0.49529092007682607
2314	80-81	-0.5359337768853081
2314	82-83	-0.5333708396747667
2314	84-85	-0.551942163925748
2314	86-87	-0.520635789209603
2314	88-89	-0.5041921023689078
2314	90-91	-0.5109986474243691
2314	92-93	-0.5456560871740557
2314	94-95	-0.5387920056598041
2314	96-97	-0.6004952904228311
2314	98-99	-0.6602906047686012
2314	100	-0.7704861335305822
2315	1	-0.3819103883106365
2315	2	-0.4589932753588215
2315	3	-0.37045122934030417
2315	4	-0.2347639370807002
2315	5	-0.2869788531701545
2315	6	-0.26659938380787196
2315	7	-0.31633305717922866
2315	8	-0.3372401204737443
2315	9	-0.33015722302627637
2315	10-11	-0.3901034953707949
2315	12-13	-0.423009147133115
2315	14-15	-0.49966324116577
2315	16-17	-0.6274871140780007
2315	18-19	-0.5329501696869627
2315	20-21	-0.523586626947619
2315	22-23	-0.4557714931942982
2315	24-25	-0.5124827389968303
2315	26-27	-0.5358161922548206
2315	28-29	-0.5403465506795584
2315	30-31	-0.5872215419207336
2315	32-33	-0.5089549302514769
2315	34-35	-0.46827833844648836
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2315	64-65	-0.5604907621943482
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2315	80-81	-0.622024266172545
2315	82-83	-0.6139149596795335
2315	84-85	-0.64032749146455
2315	86-87	-0.6527662832441905
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2315	90-91	-0.6720215775796561
2315	92-93	-0.772387442841719
2315	94-95	-0.773841900539427
2315	96-97	-0.6980100922485022
2315	98-99	-0.7213482568564196
2315	100	-0.770015322315146
2316	1	-0.4856530924035596
2316	2	-0.4312582088468204
2316	3	-0.3937716488102083
2316	4	-0.26938122106097495
2316	5	-0.36665892070925565
2316	6	-0.28633268192406547
2316	7	-0.3352535652622777
2316	8	-0.3402577439072658
2316	9	-0.41854246678213514
2316	10-11	-0.5062143688137226
2316	12-13	-0.4940235032060798
2316	14-15	-0.7014545600016433
2316	16-17	-0.7255172909421859
2316	18-19	-0.7507765216789366
2316	20-21	-0.6999215667212653
2316	22-23	-0.6162890656535822
2316	24-25	-0.5401847125375738
2316	26-27	-0.5562804872641536
2316	28-29	-0.6174807371594824
2316	30-31	-0.7387135475201703
2316	32-33	-0.6114130836272054
2316	34-35	-0.5805758411015418
2316	36-37	-0.6190901437261616
2316	38-39	-0.6178167272770878
2316	40-41	-0.5999316228712956
2316	42-43	-0.6346893752445482
2316	44-45	-0.6866650776574161
2316	46-47	-0.6787837001024855
2316	48-49	-0.6803707534328254
2316	50-51	-0.6889478626758319
2316	52-53	-0.6974724391054892
2316	54-55	-0.7880379313735162
2316	56-57	-0.7303927606811627
2316	58-59	-0.8000256219792163
2316	60-61	-0.7032364427224564
2316	62-63	-0.6936003863012274
2316	64-65	-0.6990803577026625
2316	66-67	-0.6550707130034183
2316	68-69	-0.6055458228879402
2316	70-71	-0.6154465296300557
2316	72-73	-0.62547929139361
2316	74-75	-0.6421416181244339
2316	76-77	-0.6520035862928282
2316	78-79	-0.714265327649688
2316	80-81	-0.6527418930219255
2316	82-83	-0.639429556298154
2316	84-85	-0.6450022462579739
2316	86-87	-0.7070786786078536
2316	88-89	-0.6573202575814499
2316	90-91	-0.6993422220978793
2316	92-93	-0.7278390706072351
2316	94-95	-0.7248966636539542
2316	96-97	-0.7293442290314722
2316	98-99	-0.6896358084912642
2316	100	-0.7992950765912106
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	50.0
3	63.0
4	72.0
5	179.0
6	410.0
7	1028.0
8	2282.0
9	3956.0
10	6995.0
11	12563.0
12	20492.0
13	28830.0
14	33280.0
15	43033.0
16	56829.0
17	75721.0
18	75717.0
19	48356.0
20	33761.0
21	29418.0
22	31484.0
23	35148.0
24	39437.0
25	44233.0
26	53608.0
27	67610.0
28	84889.0
29	107725.0
30	134365.0
31	169624.0
32	215911.0
33	282635.0
34	405717.0
35	662111.0
36	1285022.0
37	2521953.0
38	2288443.0
39	430849.0
40	379.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.926599642732334	18.607637437383346	13.83439441587679	23.63136850400753
2	15.180715219724982	22.56373363417697	36.18301850021129	26.07253264588676
3	19.39908731483352	27.310997175787534	29.56761312522068	23.722302384158265
4	13.890373676962756	18.68226245917547	34.40160933527601	33.02575452858577
5	13.86666292414822	37.279897597838826	32.82310450904193	16.030334968971022
6	31.35181266095418	34.220324489205154	17.977844433650183	16.450018416190478
7	25.755004886343503	32.67537859252309	22.19480922690782	19.374807294225587
8	28.04009094319821	31.189323794768004	21.202199058128095	19.56838620390569
9	28.14197457987195	14.750468654015384	19.589545003320058	37.51801176279261
10-11	25.25864775465075	25.936420816780583	25.674587187518465	23.13034424105021
12-13	27.33087244428677	23.89282892660781	26.204547264855794	22.57175136424963
14-15	25.88936458607417	23.289284621522548	25.00914921316767	25.812201579235616
16-17	23.555572863596435	26.308613772698546	27.06429188442033	23.071521479284684
18-19	24.816352334399454	25.495721208659187	28.382279617980288	21.305646838961074
20-21	24.809100746626644	25.163603634509013	27.537719834725312	22.489575784139035
22-23	23.53805436328429	26.094236106780937	25.494614905130064	24.873094624804715
24-25	23.716022676609338	26.77782989843222	25.077684148084156	24.42846327687429
26-27	24.462553248110645	25.32115459238104	25.724518203831227	24.491773955677086
28-29	23.861943029935578	24.546026712910432	28.03404942281092	23.557980834343066
30-31	25.389984278922807	24.69433143424778	26.974808993304666	22.940875293524744
32-33	22.54212324905514	25.893331069225717	27.43775958337563	24.126786098343516
34-35	22.533744123989283	26.412860679454457	27.638333164149582	23.41506203240668
36-37	24.29902351678593	26.396995179134418	26.274526315699255	23.029454988380405
38-39	24.686014436366623	24.997924208485962	26.131043887964474	24.18501746718294
40-41	23.600674537773905	26.042480495298125	26.16332776968052	24.193517197247445
42-43	25.85574397605641	25.09627365808403	25.88540990650304	23.16257245935652
44-45	25.435975527936193	24.924890082426863	26.583460406988163	23.055673982648774
46-47	24.134989797224218	25.235426111660093	27.774959222912372	22.85462486820332
48-49	24.68684837917946	26.155815673723332	26.973434667750283	22.183901279346923
50-51	23.55994397055374	26.920093008152275	25.469394458690118	24.050568562603864
52-53	23.12966714371006	27.137265552994005	24.240741648459466	25.492325654836474
54-55	24.06802814095938	26.198401071138544	24.173354764390343	25.560216023511735
56-57	24.83059402973015	24.841942233890236	24.73543628379855	25.59202745258106
58-59	24.284103179158418	24.850717750726236	26.6378932690589	24.227285801056446
60-61	24.322726631230385	25.536915603276388	25.728195654738755	24.41216211075447
62-63	22.2278197886927	26.11657076221034	26.422214319915494	25.233395129181464
64-65	22.37261869864763	26.075978362792647	26.06095192912623	25.490451009433496
66-67	24.04335258877071	25.83171383391757	25.089633132493976	25.035300444817743
68-69	24.29721434470337	24.920255689611313	25.16072468638083	25.62180527930449
70-71	23.223941295231015	25.480784837208255	25.520745562136515	25.774528305424216
72-73	24.554467240890556	24.755552379628	25.653217351506402	25.03676302797504
74-75	24.56345408665751	24.386675269902415	26.011669008985987	25.038201634454083
76-77	23.21763442454557	25.44998523690166	26.693358448456216	24.639021890096554
78-79	23.44306454674011	25.34654379701964	26.519633915146624	24.690757741093634
80-81	22.565302482982432	25.479951207272883	25.966962489894662	25.98778381985002
82-83	22.58587574035244	25.968655895800204	24.71913639369244	26.72633197015492
84-85	23.00442893917851	25.548547319626962	24.685728837460914	26.761294903733607
86-87	23.00308277422897	24.301806543195	25.464138309696942	27.230972372879087
88-89	23.061202101139543	23.77827151907298	26.32106240156466	26.839463978222817
90-91	23.46233092153868	24.406955850616146	25.775273276732435	26.355439951112736
92-93	22.05787925344097	24.916996590299583	26.10112196622611	26.924002190033335
94-95	21.472436970774613	25.14672902192544	26.460104158508805	26.92072984879114
96-97	22.706053514160075	24.739684803086295	25.4738908086583	27.080370874095326
98-99	22.81314969566683	24.12787178474634	25.337164129503424	27.72181439008341
100	22.90903605654783	24.5782889349136	25.685603221558424	26.82707178698015
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	884.0
1	570.0
2	351.0
3	477.5
4	425.5
5	493.0
6	856.5
7	1676.5
8	2694.5
9	2963.0
10	2414.5
11	2135.5
12	2396.0
13	2712.5
14	3180.0
15	3830.0
16	4363.5
17	4955.5
18	5816.5
19	6744.0
20	7716.5
21	8836.5
22	10499.5
23	12513.5
24	14879.0
25	18208.5
26	23038.5
27	28587.5
28	34115.5
29	43239.0
30	55146.5
31	65935.0
32	78431.0
33	91914.5
34	105664.5
35	118150.0
36	129512.0
37	145253.5
38	155927.0
39	161994.0
40	166904.5
41	170119.5
42	177328.5
43	196021.5
44	221440.5
45	246288.0
46	285079.5
47	337444.0
48	501838.0
49	653467.5
50	751601.5
51	701760.5
52	506699.5
53	417150.0
54	363612.5
55	321895.5
56	291481.0
57	259544.5
58	241787.5
59	223722.5
60	190463.5
61	155957.5
62	127877.5
63	100821.5
64	74761.0
65	59421.5
66	46215.5
67	34048.5
68	26364.5
69	21798.0
70	18965.0
71	16314.5
72	13858.5
73	14176.5
74	11425.5
75	8520.5
76	6465.5
77	4189.0
78	2878.0
79	1729.0
80	1153.5
81	868.0
82	604.5
83	398.5
84	267.5
85	158.0
86	101.0
87	54.5
88	25.5
89	18.0
90	12.0
91	8.0
92	4.5
93	3.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.06153728801829148
3	0.025422699245718258
4	0.006802955761075051
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	6.96365550346265E-5
12-13	1.874830327855329E-4
14-15	4.49959278685279E-4
16-17	4.820992271627989E-4
18-19	0.0
20-21	0.0028122454917829938
22-23	0.0048424189039463356
24-25	7.124355245850251E-4
26-27	4.713859110036256E-4
28-29	0.0048370622458667484
30-31	0.0022712230257447416
32-33	0.0011838214355886507
34-35	0.0015587875011597164
36-37	0.004038920192008338
38-39	0.004167479985918417
40-41	8.83848583131798E-4
42-43	0.0025551259039628344
44-45	2.624762458997461E-4
46-47	0.002126593257595902
48-49	1.6069974238759966E-5
50-51	0.0016873472950697964
52-53	0.006915445580746371
54-55	0.013980877587721168
56-57	0.02469419374689448
58-59	0.027061836618071777
60-61	0.02475847364384952
62-63	0.008109980332494195
64-65	0.006706535915642492
66-67	1.9283969086511958E-4
68-69	0.0013552344941354236
70-71	1.39273110069253E-4
72-73	7.713587634604783E-4
74-75	0.005147748414482775
76-77	0.001007051718962291
78-79	0.0012052480679069974
80-81	0.0
82-83	0.001178464777509064
84-85	1.6069974238759966E-5
86-87	5.678057564361854E-4
88-89	5.356658079586655E-5
90-91	5.356658079586654E-4
92-93	7.874287376992382E-4
94-95	0.0014462976814883968
96-97	1.39273110069253E-4
98-99	0.0
100	6.642256018687451E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	9334178.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.98415581109257
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.4545915016265	24.558218469408974
2	13.775479304160104	9.08745111481796
3	4.411789471105794	4.365574539620736
4	2.0491634462332797	2.7035970557181566
5	1.1829629536653812	1.950951719122461
6	0.7252983569172089	1.435401240845199
7	0.5179437239141325	1.195875554367288
8	0.3812127323346179	1.0059184128397891
9	0.28867316378162744	0.8569476551388818
>10	1.8589536464699272	11.56761159223333
>50	0.17161397991179733	3.953373196639598
>100	0.14532394222483502	10.046209836448726
>500	0.02164242783274217	4.939121489965595
>1k	0.013232798364688915	7.850552478260843
>5k	8.474205829660628E-4	1.9584507743170763
>10k+	0.0012711308744490942	12.524744870255427
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	120149	1.2871942232085138	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	103759	1.1116029713596633	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	72412	0.7757726497180577	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	67064	0.7184778348987988	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	65078	0.6972011890066806	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	53671	0.5749943915789907	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	48449	0.5190494545957877	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	42791	0.45843351176718505	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	40758	0.4366533400155857	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	38182	0.40905583758955527	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	34314	0.3676167306858729	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	33533	0.35924963076555855	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	31018	0.3323056406252377	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	27539	0.29503401370747373	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25881	0.2772713355155644	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	24364	0.2610192349020985	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	20910	0.2240154408883139	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	19736	0.21143800771744442	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC	18922	0.20271736836387733	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC	18831	0.2017424565933926	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18008	0.19292539739439296	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	17700	0.18962569601736756	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	17668	0.18928286990027404	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT	17357	0.18595102857477114	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15489	0.16593855398943538	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG	14371	0.15396106652347963	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	14348	0.15371466025181865	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	14212	0.15225764925417107	No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	13471	0.14431908198022364	No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	13466	0.14426551539942778	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA	13175	0.14114794039710835	No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG	13101	0.14035515500132953	No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	12097	0.12959898557751953	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	11458	0.12275317655180777	No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA	10880	0.1165608798118056	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC	10282	0.11015431674861996	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	10221	0.1095008044629104	No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC	10217	0.1094579511982737	No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	10150	0.10874015901560909	No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	9708	0.10400487327325447	No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA	9347	0.1001373661397929	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.003299701377025379	0.0	0.0	1.0713316159173308E-5	0.0
2	0.005206671653358228	0.0	0.0	2.1426632318346617E-5	0.0
3	0.005442364608860041	0.0	0.0	3.2139948477519925E-5	0.0
4	0.006331569850071426	0.0	0.0	3.2139948477519925E-5	0.0
5	0.02515486634173893	0.0	0.0	1.39273110069253E-4	0.0
6	0.05298806172327119	0.0	0.0	4.606725948444523E-4	1.0713316159173308E-5
7	0.059266064992546746	0.0	0.0	5.356658079586654E-4	1.0713316159173308E-5
8	0.06898304274891694	0.0	0.0	6.213723372320519E-4	1.0713316159173308E-5
9	0.0754217457605801	0.0	0.0	7.606454473013049E-4	1.0713316159173308E-5
10-11	0.1409604573643228	0.0	0.0	0.0015641441592393031	2.1426632318346617E-5
12-13	0.24595631238230084	0.0	0.0	0.003262204770468273	3.2139948477519925E-5
14-15	0.36779350040249925	0.0	0.0	0.005088825175607322	5.8923238875453196E-5
16-17	0.4209744018166356	0.0	0.0	0.00592446383602284	7.499321311421316E-5
18-19	0.44532041278835693	0.0	0.0	0.006636899360607865	8.570652927338647E-5
20-21	0.5334052982490798	0.0	0.0	0.008436736475348981	1.1248981967131975E-4
22-23	0.5572531400193996	0.0	0.0	0.01023121693201051	1.178464777509064E-4
24-25	0.6672896102902688	0.0	0.0	0.019053632789089732	1.285597939100797E-4
26-27	0.8905872589959181	0.0	0.0	0.049286610990276813	1.4998642622842632E-4
28-29	0.9434414042671995	0.0	0.0	0.07575385856151448	1.4998642622842632E-4
30-31	0.9615361952600432	0.0	0.0	0.1335361292660157	1.4998642622842632E-4
32-33	0.9777400859507929	0.0	0.0	0.22583134797729376	1.6069974238759962E-4
34-35	0.9908638982457803	0.0	0.0	0.34903448380778684	1.6069974238759962E-4
36-37	1.012263747273729	0.0	0.0	0.5284503895254622	1.6069974238759962E-4
38-39	1.0349331242665396	0.0	0.0	0.7948048558748291	1.7141305854677293E-4
40-41	1.0559847905193152	0.0	0.0	1.1763221142772293	1.9283969086511958E-4
42-43	1.0998236802426522	0.0	0.0	1.4542844586850605	1.9819634894470623E-4
44-45	1.1473265240924269	0.0	0.0	1.7363874997884121	2.0355300702429288E-4
46-47	1.1652445453686442	0.0	0.0	2.059929647795446	2.0890966510387954E-4
48-49	1.1770988296987694	5.356658079586654E-6	0.0	2.354229799346016	2.249796393426395E-4
50-51	1.1989486380054033	1.0713316159173308E-5	0.0	2.6820679871328785	2.249796393426395E-4
52-53	1.20573016713416	1.0713316159173308E-5	0.0	3.0804265785375	2.249796393426395E-4
54-55	1.2298083452019022	1.0713316159173308E-5	0.0	3.427741575101739	2.249796393426395E-4
56-57	1.276346990597351	1.0713316159173308E-5	0.0	3.792872816438684	2.249796393426395E-4
58-59	1.2920741387190173	1.0713316159173308E-5	0.0	4.170340441332916	2.249796393426395E-4
60-61	1.3035588136416512	1.0713316159173308E-5	0.0	4.510156116585735	2.356929555018128E-4
62-63	1.3132168681591458	1.0713316159173308E-5	0.0	4.865806073121812	2.356929555018128E-4
64-65	1.3208822458710343	2.1426632318346617E-5	0.0	5.2559046977677095	2.356929555018128E-4
66-67	1.3326133270653293	3.749660655710658E-5	0.0	5.658961078308128	2.356929555018128E-4
68-69	1.3450247038357315	4.285326463669323E-5	0.0	6.150198764154702	2.356929555018128E-4
70-71	1.3610411114936953	4.285326463669323E-5	0.0	6.732183594527552	2.571195878201594E-4
72-73	1.398757341032065	4.285326463669323E-5	0.0	7.2036766386927695	2.9997285245685263E-4
74-75	1.4344969637390674	5.356658079586655E-5	0.0	7.660508509694158	2.9997285245685263E-4
76-77	1.4455584626734137	6.427989695503985E-5	0.0	8.159749042711635	2.9997285245685263E-4
78-79	1.4536363030574306	6.427989695503985E-5	0.0	8.648056636588674	3.1604282669561257E-4
80-81	1.4692134647528685	6.427989695503985E-5	0.0	9.203006413633851	3.2675614285478593E-4
82-83	1.4759414273008293	6.427989695503985E-5	0.0	9.859004188692353	3.3211280093437256E-4
84-85	1.4995642894318064	6.427989695503985E-5	0.0	10.461837132310954	3.374694590139592E-4
86-87	1.5375322818999166	6.427989695503985E-5	0.0	11.054235305990522	3.4282611709354587E-4
88	1.5471956930754909	6.427989695503985E-5	0.0	11.527731740277504	3.4282611709354587E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGATC	2920	0.0	28.494143	3
CGATCTC	2905	0.0	24.42636	5
GATCTAC	4145	0.0	23.376165	1
TCTCGCG	1360	0.0	21.769018	8
TCTACAC	5420	0.0	20.641577	3
TATCACG	595	0.0	20.553555	2
AGTACTC	3770	0.0	20.193033	5
TACACTC	5325	0.0	19.76771	5
CCGATCT	4455	0.0	18.777285	4
GTATAAG	2770	0.0	18.50876	1
AGTACTT	34635	0.0	18.425705	12-13
ATCTACA	5465	0.0	17.729954	2
CTCGCGC	1750	0.0	17.18617	9
CTACACT	7615	0.0	16.971619	4
GAGTACT	33445	0.0	16.580223	12-13
CATGGGT	8610	0.0	16.374899	4
TACTTTT	39890	0.0	16.12283	14-15
GTACTAG	2395	0.0	15.907797	1
TAGTACT	2795	0.0	15.805437	4
CGTTAAC	945	0.0	15.429806	1
>>END_MODULE