##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765531_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7924068
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.29914382360172	33.0	31.0	34.0	31.0	34.0
2	32.32296315478363	34.0	31.0	34.0	31.0	34.0
3	32.34514948634969	34.0	31.0	34.0	31.0	34.0
4	35.81813558389454	37.0	35.0	37.0	35.0	37.0
5	35.85036473690029	37.0	35.0	37.0	35.0	37.0
6	35.95394019839305	37.0	37.0	37.0	35.0	37.0
7	35.9140499551493	37.0	37.0	37.0	35.0	37.0
8	35.94420441621652	37.0	37.0	37.0	35.0	37.0
9	37.64756233793047	39.0	38.0	39.0	35.0	39.0
10-11	37.58433736308169	39.0	38.0	39.0	35.0	39.0
12-13	37.5677201407156	39.0	38.0	39.0	35.0	39.0
14-15	38.95485992800668	41.0	39.0	41.0	36.0	41.0
16-17	38.90893686172304	40.5	39.0	41.0	36.0	41.0
18-19	38.896591435106316	41.0	39.0	41.0	35.5	41.0
20-21	38.87759254463742	41.0	39.0	41.0	35.0	41.0
22-23	38.7825514622035	40.5	39.0	41.0	35.0	41.0
24-25	38.707981241453254	40.5	39.0	41.0	35.0	41.0
26-27	38.580722805508486	40.0	38.0	41.0	35.0	41.0
28-29	38.437633107136385	40.0	38.0	41.0	34.0	41.0
30-31	38.21678524212564	40.0	38.0	41.0	34.0	41.0
32-33	37.95282429428924	40.0	38.0	41.0	34.0	41.0
34-35	37.80943512852237	40.0	38.0	41.0	33.0	41.0
36-37	37.658405695155565	40.0	37.0	41.0	33.0	41.0
38-39	37.46514132639952	40.0	37.0	41.0	33.0	41.0
40-41	37.215080877650216	40.0	37.0	41.0	32.0	41.0
42-43	36.95216011523374	40.0	36.0	41.0	31.5	41.0
44-45	36.599462170188346	40.0	36.0	41.0	31.0	41.0
46-47	36.18019481912573	39.0	35.0	41.0	30.0	41.0
48-49	35.784308640460935	39.0	35.0	41.0	28.5	41.0
50-51	34.52920406538662	38.0	34.0	40.0	26.0	40.5
52-53	34.47026792299107	38.0	34.0	39.5	26.0	40.0
54-55	35.278420818700695	39.0	35.0	40.5	28.0	41.0
56-57	35.288294093387385	39.0	35.0	41.0	28.0	41.0
58-59	35.08149892201834	38.0	35.0	41.0	27.5	41.0
60-61	34.73835787375878	38.0	35.0	40.0	27.0	41.0
62-63	34.293157125860105	37.0	34.5	40.0	26.0	41.0
64-65	34.00337187919135	36.5	34.0	39.5	26.0	41.0
66-67	33.70984158641748	36.0	34.0	39.0	26.0	41.0
68-69	33.47599623072392	35.5	34.0	39.0	25.0	41.0
70-71	33.1064175622925	35.0	34.0	38.5	24.0	40.5
72-73	32.72080817580061	35.0	34.0	37.0	23.5	39.5
74-75	32.33025599982231	35.0	34.0	37.0	22.0	39.0
76-77	31.963058620900277	35.0	33.0	36.0	20.0	39.0
78-79	31.675062859127408	35.0	33.0	36.0	20.0	37.0
80-81	31.385382798330355	35.0	33.0	35.5	18.5	37.0
82-83	31.143760376614637	35.0	33.0	35.0	17.5	36.5
84-85	30.905003843985185	35.0	33.0	35.0	13.0	36.0
86-87	30.65102470094906	35.0	33.0	35.0	7.0	36.0
88-89	30.443470765268547	35.0	32.5	35.0	2.0	36.0
90-91	30.221603916069373	35.0	32.0	35.0	2.0	35.0
92-93	29.954660156879015	35.0	32.0	35.0	2.0	35.0
94-95	29.688412189294688	35.0	32.0	35.0	2.0	35.0
96-97	29.439311033171343	35.0	31.5	35.0	2.0	35.0
98-99	29.135597271502466	35.0	31.0	35.0	2.0	35.0
100	28.598717098338884	34.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.40229326221381
1101	2	0.5180210230914284
1101	3	0.5240094276843621
1101	4	0.34127816291329793
1101	5	0.3925408898552263
1101	6	0.37838110923402724
1101	7	0.46406674719180074
1101	8	0.41281648842105056
1101	9	0.5445779294280939
1101	10-11	0.5216717449936965
1101	12-13	0.5392360059453623
1101	14-15	0.7140899828733538
1101	16-17	0.6715195218742807
1101	18-19	0.684375109998868
1101	20-21	0.6698070789767954
1101	22-23	0.6785306808101694
1101	24-25	0.6614365658390611
1101	26-27	0.6687917115486783
1101	28-29	0.7024647430486866
1101	30-31	0.7449103479339314
1101	32-33	0.7382472658402648
1101	34-35	0.8484653803407696
1101	36-37	0.9060059256118578
1101	38-39	0.8379216542347123
1101	40-41	0.8453643395201524
1101	42-43	0.8822389721799624
1101	44-45	0.9293385531077192
1101	46-47	0.9933274154304215
1101	48-49	1.1534106063302403
1101	50-51	1.037422070836726
1101	52-53	1.0792529256826597
1101	54-55	1.2424954819978424
1101	56-57	1.2922468990049794
1101	58-59	1.3283915164707238
1101	60-61	1.3007355508961709
1101	62-63	1.293551709512407
1101	64-65	1.289296282269241
1101	66-67	1.2832323229986997
1101	68-69	1.2861275186099235
1101	70-71	1.2318121982007213
1101	72-73	1.2041191689656117
1101	74-75	1.1520410216947141
1101	76-77	1.0787354528411868
1101	78-79	1.1573612006131846
1101	80-81	1.2282711660333376
1101	82-83	1.2404273159430481
1101	84-85	1.2140176785765782
1101	86-87	1.2015900444357577
1101	88-89	1.190823468334603
1101	90-91	1.2130471044793687
1101	92-93	1.2566177228780013
1101	94-95	1.2178498921109906
1101	96-97	1.2658536451817
1101	98-99	1.2372515579826064
1101	100	1.3269685943762042
1102	1	0.3454980123384672
1102	2	0.46516319790436
1102	3	0.5040957460764446
1102	4	0.3310534123629978
1102	5	0.3486877415722276
1102	6	0.36539779075762624
1102	7	0.37831434644299833
1102	8	0.39044030003456953
1102	9	0.4846192163539058
1102	10-11	0.4349065672802297
1102	12-13	0.5036033362038594
1102	14-15	0.6522779916695285
1102	16-17	0.6740016942249767
1102	18-19	0.587424512879366
1102	20-21	0.6223866497877992
1102	22-23	0.6620435231022554
1102	24-25	0.6661149515703713
1102	26-27	0.6629882126393056
1102	28-29	0.7224107516244871
1102	30-31	0.7438272598141182
1102	32-33	0.713760395842364
1102	34-35	0.7660654208572382
1102	36-37	0.8027725822841063
1102	38-39	0.8257831471856818
1102	40-41	0.894861776395679
1102	42-43	0.8460543194618353
1102	44-45	0.9013636032751364
1102	46-47	0.8367900000630968
1102	48-49	0.9421495609461417
1102	50-51	0.9787352892530805
1102	52-53	0.9865649086700543
1102	54-55	1.0465382926014044
1102	56-57	1.0591761953465664
1102	58-59	1.1007266900167068
1102	60-61	1.1020672064598713
1102	62-63	1.1295460910853876
1102	64-65	1.107193892525082
1102	66-67	1.132073306913064
1102	68-69	1.1449990218758899
1102	70-71	1.0609493564070078
1102	72-73	1.1401139276865564
1102	74-75	1.0977621568777423
1102	76-77	1.0409232719858181
1102	78-79	1.1221650796338203
1102	80-81	1.0899848832548003
1102	82-83	1.104093447329003
1102	84-85	1.1379041020851837
1102	86-87	1.1637405528716158
1102	88-89	1.1467231534429985
1102	90-91	1.1428760328592453
1102	92-93	1.2141807651797265
1102	94-95	1.146589671064575
1102	96-97	1.2118094202732905
1102	98-99	1.3126292918675446
1102	100	1.3325883683514306
1103	1	0.35986230649037054
1103	2	0.43513660706108936
1103	3	0.46249718695615627
1103	4	0.3400016447474812
1103	5	0.35911091913322934
1103	6	0.3393453261966073
1103	7	0.4236159380847866
1103	8	0.32751039725011566
1103	9	0.4486305674111719
1103	10-11	0.4244636106329125
1103	12-13	0.44481548147162897
1103	14-15	0.5179348958870307
1103	16-17	0.5392928523987237
1103	18-19	0.5565285109142835
1103	20-21	0.537297053867853
1103	22-23	0.604690487393384
1103	24-25	0.5575752107752976
1103	26-27	0.4987384367243237
1103	28-29	0.4996820021478712
1103	30-31	0.6049048043902303
1103	32-33	0.647707936627036
1103	34-35	0.6048802005389291
1103	36-37	0.589741097854322
1103	38-39	0.63809194656708
1103	40-41	0.6602382786540417
1103	42-43	0.6539376485119348
1103	44-45	0.5832029674256134
1103	46-47	0.5940296468139579
1103	48-49	0.5888817150984593
1103	50-51	0.5587413258836733
1103	52-53	0.6461476554799788
1103	54-55	0.6760950165485795
1103	56-57	0.6673881445608174
1103	58-59	0.697720790102359
1103	60-61	0.7107615381880166
1103	62-63	0.7961990593983543
1103	64-65	0.7757838080510098
1103	66-67	0.7337082087680358
1103	68-69	0.6578281983268539
1103	70-71	0.7196935635068797
1103	72-73	0.656735276189913
1103	74-75	0.5836704265538231
1103	76-77	0.6304473256935239
1103	78-79	0.6377150832354808
1103	80-81	0.6423531548740442
1103	82-83	0.6302684620396981
1103	84-85	0.6546134875690193
1103	86-87	0.5871261952061317
1103	88-89	0.6473812758116608
1103	90-91	0.691087388700911
1103	92-93	0.6514050858115432
1103	94-95	0.696008419847594
1103	96-97	0.722381275087848
1103	98-99	0.7506171787808888
1103	100	0.836919654148403
1104	1	0.3537088186623123
1104	2	0.37137261900971197
1104	3	0.44371672587359257
1104	4	0.37274321988734727
1104	5	0.4119411142051064
1104	6	0.34720237526207853
1104	7	0.4176354006361649
1104	8	0.4037080599143792
1104	9	0.4801915099836478
1104	10-11	0.4799584037430904
1104	12-13	0.42903866420535763
1104	14-15	0.5093042779888961
1104	16-17	0.5169803525735475
1104	18-19	0.5175826265364591
1104	20-21	0.47879421438900494
1104	22-23	0.47831004099781893
1104	24-25	0.55736796579329
1104	26-27	0.5429154889824872
1104	28-29	0.5511222119607098
1104	30-31	0.5166426807163731
1104	32-33	0.6075400140215024
1104	34-35	0.6313366741878568
1104	36-37	0.6473377403792426
1104	38-39	0.6108552657850552
1104	40-41	0.666679705225981
1104	42-43	0.6245109096820229
1104	44-45	0.6480807716791119
1104	46-47	0.6235167409926916
1104	48-49	0.6241773418609853
1104	50-51	0.5388601183685537
1104	52-53	0.6173866771197751
1104	54-55	0.7417770105191082
1104	56-57	0.7084697198968826
1104	58-59	0.6962558334146038
1104	60-61	0.690354210891087
1104	62-63	0.7262389368706934
1104	64-65	0.704374644998083
1104	66-67	0.651263452330042
1104	68-69	0.6589959581068996
1104	70-71	0.6989323872189459
1104	72-73	0.7013582961690688
1104	74-75	0.6927790887558487
1104	76-77	0.7011064494386474
1104	78-79	0.7020232247898868
1104	80-81	0.7553212500990298
1104	82-83	0.6609774368603709
1104	84-85	0.5963890528951517
1104	86-87	0.5673141185972277
1104	88-89	0.5503973807224938
1104	90-91	0.4844480974397456
1104	92-93	0.5514542643898217
1104	94-95	0.6218573333902526
1104	96-97	0.6681579627398158
1104	98-99	0.6645080804996475
1104	100	0.7368333877598303
1105	1	0.22164455628102075
1105	2	0.29607698695767937
1105	3	0.2894948319654347
1105	4	0.20832870334387366
1105	5	0.25211846004249594
1105	6	0.27636604966591705
1105	7	0.26991182280024617
1105	8	0.2989221793876524
1105	9	0.3873974412900836
1105	10-11	0.34781242314736716
1105	12-13	0.38242051877207217
1105	14-15	0.41469504378672895
1105	16-17	0.4140537613488178
1105	18-19	0.427759571647897
1105	20-21	0.4019783107010326
1105	22-23	0.32956044655708183
1105	24-25	0.34973571832181705
1105	26-27	0.345307480158084
1105	28-29	0.37441569063805247
1105	30-31	0.43984307848960214
1105	32-33	0.48080425552286243
1105	34-35	0.4511434768271627
1105	36-37	0.478223139085749
1105	38-39	0.45982543525347097
1105	40-41	0.4845933501905293
1105	42-43	0.4825267643689486
1105	44-45	0.5234152196633133
1105	46-47	0.473331545847941
1105	48-49	0.6186159423853752
1105	50-51	0.5525758075275462
1105	52-53	0.6031597020769155
1105	54-55	0.6219276483236698
1105	56-57	0.6732246601623544
1105	58-59	0.6686666253158648
1105	60-61	0.681389707828167
1105	62-63	0.7268074843832508
1105	64-65	0.6396658497314291
1105	66-67	0.5851409029537464
1105	68-69	0.5879850443005878
1105	70-71	0.5621903700137452
1105	72-73	0.5240985621315843
1105	74-75	0.5781510714037097
1105	76-77	0.5977139088466927
1105	78-79	0.6074262320623696
1105	80-81	0.5154257218139264
1105	82-83	0.5275648810619167
1105	84-85	0.5618194448648843
1105	86-87	0.5191312262206189
1105	88-89	0.5530573310113844
1105	90-91	0.6649617525278941
1105	92-93	0.6398560339815447
1105	94-95	0.6033248304735608
1105	96-97	0.5629447554453364
1105	98-99	0.6161611277568362
1105	100	0.6779636177167632
1106	1	0.15857177500888042
1106	2	0.2805787643572657
1106	3	0.3312158635687439
1106	4	0.23249149306682426
1106	5	0.32350042599167494
1106	6	0.25472027609502135
1106	7	0.23931881367784769
1106	8	0.26792100680103204
1106	9	0.2435825676672394
1106	10-11	0.31127828411153047
1106	12-13	0.3736475872957712
1106	14-15	0.395696654996776
1106	16-17	0.42183238225828745
1106	18-19	0.4580961810108519
1106	20-21	0.42510324429832025
1106	22-23	0.47663317129553917
1106	24-25	0.46820732037925694
1106	26-27	0.49414856396732176
1106	28-29	0.505417642999646
1106	30-31	0.5213201574915303
1106	32-33	0.47964831222419946
1106	34-35	0.42152155559808335
1106	36-37	0.45236431442106806
1106	38-39	0.46078918838841076
1106	40-41	0.4741521632560506
1106	42-43	0.44211614461354287
1106	44-45	0.3971274212897171
1106	46-47	0.4355079757846738
1106	48-49	0.4562772948855667
1106	50-51	0.3623468753416432
1106	52-53	0.38356671049945845
1106	54-55	0.4456798865215248
1106	56-57	0.48419156163875954
1106	58-59	0.4910254056308361
1106	60-61	0.4622939446380343
1106	62-63	0.5619631274685517
1106	64-65	0.5132945179634874
1106	66-67	0.49534043274942974
1106	68-69	0.5276816094892851
1106	70-71	0.46406129386190287
1106	72-73	0.4520576375913876
1106	74-75	0.4622273109778732
1106	76-77	0.4458536338005672
1106	78-79	0.3467669761821277
1106	80-81	0.3924490429340324
1106	82-83	0.3774411161523794
1106	84-85	0.383238206445764
1106	86-87	0.45262078259688465
1106	88-89	0.45320361192337444
1106	90-91	0.4670412905502097
1106	92-93	0.5122057737803836
1106	94-95	0.5284249584541314
1106	96-97	0.5203368029102435
1106	98-99	0.512208545873154
1106	100	0.5456357067436741
1107	1	0.1623797214711331
1107	2	0.22411431623047662
1107	3	0.19657655031168986
1107	4	0.23251025096291755
1107	5	0.24684495044486
1107	6	0.20185112257104976
1107	7	0.27383105037499433
1107	8	0.20740143833929636
1107	9	0.2823713707843609
1107	10-11	0.29242096181476995
1107	12-13	0.3337067406251535
1107	14-15	0.32612338450286416
1107	16-17	0.3543295203927457
1107	18-19	0.3531362097062214
1107	20-21	0.2943281745806914
1107	22-23	0.2999920518542396
1107	24-25	0.39156936311695034
1107	26-27	0.30816479604955305
1107	28-29	0.3390013310957798
1107	30-31	0.34343584796172877
1107	32-33	0.3208403620197231
1107	34-35	0.349360069665849
1107	36-37	0.4107501324412297
1107	38-39	0.42545216559676646
1107	40-41	0.41983381023113253
1107	42-43	0.44284134078090887
1107	44-45	0.42435537285636826
1107	46-47	0.448140342913284
1107	48-49	0.48761280469618384
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2316	100	-0.585247415779147
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	20503.0
3	3670.0
4	3507.0
5	3999.0
6	4700.0
7	5247.0
8	6420.0
9	7741.0
10	10139.0
11	14580.0
12	22657.0
13	32736.0
14	34116.0
15	41117.0
16	55028.0
17	75410.0
18	75980.0
19	45082.0
20	38381.0
21	35537.0
22	36919.0
23	42522.0
24	48155.0
25	52240.0
26	57850.0
27	67889.0
28	80243.0
29	96212.0
30	117382.0
31	148634.0
32	193926.0
33	262831.0
34	384904.0
35	621926.0
36	1137801.0
37	1982787.0
38	1698818.0
39	355989.0
40	490.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.97086542608793	17.800781217428742	13.09547362348898	24.132879732994347
2	14.80798052867317	21.819046246427583	37.120648922645856	26.252324302253392
3	19.037627941422528	27.28581476373308	29.189928407647407	24.486628887196986
4	13.129692677143812	18.051266072894183	35.132256234104425	33.68678501585757
5	13.56440836627064	36.84459732778728	33.45041329636187	16.140581009580213
6	31.11029001914557	34.242854151616065	18.451722425031093	16.195133404207272
7	25.78947840693508	32.06255037143264	22.359298378471152	19.78867284316113
8	27.674091321690014	31.067521383936423	21.454529694913745	19.803857599459818
9	27.9820176207287	14.532422479693826	19.610034463023503	37.87552543655397
10-11	24.912031349678784	25.652615666114286	26.260685881706554	23.174667102500372
12-13	27.169207715270694	23.446714997714423	26.517491681475423	22.86658560553946
14-15	25.715370236723334	22.9400489735813	25.15060251432093	26.193978275374434
16-17	23.295557897052603	26.04007548642769	27.36419648837361	23.300170128146103
18-19	24.491295905835855	25.119772213200413	28.8232856367754	21.56564624418833
20-21	24.33138433784139	24.738447737131683	28.06211634799118	22.86805157703575
22-23	23.073377179724723	25.628177637860084	26.12925608112254	25.16918910129265
24-25	23.134898342339735	26.464907893998337	25.636671896734892	24.76352186692704
26-27	24.102425837510783	24.908426019657004	26.08630280020424	24.902845342627973
28-29	23.38329164873186	24.37536620191022	28.358587438745126	23.88275471061279
30-31	24.859883136250325	24.545396371568245	27.382574745855997	23.212145746325433
32-33	22.167736588231886	25.557771148702844	27.58919943866582	24.685292824399447
34-35	22.225507525682545	26.041419084302532	28.107344257978202	23.625729132036724
36-37	23.915779573734365	25.80047089179246	26.733558116662714	23.550191417810463
38-39	24.5087844103831	24.64968609401045	26.476627478543787	24.36490201706267
40-41	23.460192513233906	25.75928109868632	26.544799506165823	24.235726881913955
42-43	25.27048587342161	25.002363169935	26.115032608587896	23.612118348055496
44-45	25.00917204405183	24.730969690160173	26.58330890718056	23.67654935860744
46-47	23.760259363093482	25.039736441319633	28.069070228248137	23.130933967338745
48-49	24.38263601079313	25.82719214859166	27.11710811046146	22.673063730153746
50-51	22.989193841977844	26.46288801940029	26.100365127059995	24.447553011561872
52-53	22.694942547627573	26.643445726146986	24.731331271528415	25.930280454697023
54-55	23.590181583182716	26.1622290918467	24.537998673159613	25.70959065181097
56-57	24.367281356646266	24.698894689577163	24.962943944926312	25.970880008850262
58-59	23.881770384496512	24.558152702683667	26.890980354208033	24.669096558611788
60-61	24.053857773646293	25.19403171700229	26.05879745172731	24.693313057624106
62-63	22.12401933602212	25.553131745913454	26.732631621494967	25.590217296569463
64-65	22.157690716002644	25.56527721422981	26.75611373052138	25.52091833924617
66-67	24.001991324525136	25.20412529074361	25.699840544979185	25.094042839752063
68-69	24.401761913780863	24.250401326279228	25.673387888487913	25.674448871452
70-71	23.18344956620441	24.97427032499023	26.00728574182107	25.834994366984294
72-73	24.691318238370393	24.268330435122188	25.863872605342376	25.17647872116504
74-75	24.927888159194016	23.757915107414846	26.18788247976228	25.126314253628856
76-77	23.550084473742018	24.829432579839146	27.27816910868744	24.342313837731393
78-79	23.81822007871329	24.700085689802915	27.034943239984532	24.44675099149927
80-81	22.935185880462488	24.904986585171233	26.61971513437596	25.54011239999032
82-83	23.017861545069074	25.445009698585086	25.17586780255498	26.361260953790865
84-85	23.424111013647934	25.25808221117265	24.98912386783319	26.328682907346224
86-87	23.75492861036194	23.900903456372887	25.65800550932429	26.686162423940885
88-89	23.920162692875486	23.39433684199883	26.617398734049225	26.068101731076464
90-91	24.470234962239083	24.0553670478672	25.88118029713048	25.593217692763236
92-93	23.271726727749044	24.39724279165324	26.28477695643216	26.046253524165554
94-95	22.84458882258451	24.574741959666945	26.789354181588532	25.79131503616002
96-97	24.461596831030384	24.116735691861756	25.62134545730566	25.800322019802195
98-99	24.751723228572704	23.549760295197455	25.445998875985936	26.252517600243902
100	24.521697539742586	23.949810652481023	25.891241804831395	25.637250002945
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1641.0
1	2085.0
2	1785.5
3	983.5
4	825.0
5	835.0
6	1123.0
7	1864.5
8	2808.0
9	3036.0
10	2584.0
11	2376.0
12	2567.5
13	2823.0
14	3143.5
15	3562.0
16	4060.5
17	4641.0
18	5380.0
19	6233.5
20	7274.5
21	8785.0
22	10532.5
23	12491.0
24	15077.5
25	18694.5
26	23770.5
27	29643.5
28	35589.5
29	43531.0
30	52694.5
31	62048.5
32	71749.5
33	81256.0
34	92085.0
35	101261.5
36	108021.0
37	116353.0
38	123191.0
39	130395.0
40	136818.0
41	142149.5
42	148608.5
43	163204.0
44	185082.5
45	204991.5
46	235804.5
47	273678.0
48	378695.0
49	506604.0
50	654001.0
51	619828.0
52	418365.5
53	348098.0
54	312545.5
55	273902.5
56	251142.0
57	226569.0
58	212095.0
59	196662.5
60	167137.5
61	135997.0
62	110530.5
63	86824.0
64	64120.0
65	51685.5
66	41761.5
67	31669.0
68	24349.0
69	19813.5
70	17108.0
71	14798.0
72	12684.0
73	12935.5
74	10794.5
75	8507.5
76	6809.5
77	4855.5
78	3669.0
79	2663.5
80	1973.0
81	1577.0
82	1296.0
83	1041.0
84	759.0
85	478.5
86	325.5
87	215.0
88	160.5
89	129.0
90	115.5
91	107.5
92	86.0
93	65.5
94	50.5
95	42.5
96	32.5
97	23.5
98	19.5
99	15.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11017068505722061
2	0.35524682524178236
3	0.36535526954084696
4	0.36329824529521954
5	0.29277891103407994
6	0.13234363965579296
7	0.1463515961750959
8	0.122613788776169
9	0.20042735625186459
10-11	0.200061382613072
12-13	0.21285153029984094
14-15	0.14430088181979256
16-17	0.13033078464243364
18-19	0.14837076107877925
20-21	0.14874935449821985
22-23	0.1568954229065172
24-25	0.16910506068347722
26-27	0.16205691319155766
28-29	0.17002630467078272
30-31	0.2112551280478663
32-33	0.20796136529873294
34-35	0.1266394988028876
36-37	0.08786522276184404
38-39	0.08190868629597828
40-41	0.08115149945709703
42-43	0.07166142440978547
44-45	0.09273645809198003
46-47	0.1387103189927194
48-49	0.21456782046797174
50-51	0.17252502123909083
52-53	0.10741326298562809
54-55	0.056814252477389145
56-57	0.07141533868714907
58-59	0.1054067178625928
60-61	0.08263432368323946
62-63	0.09134197233037374
64-65	0.10035249571306053
66-67	0.0975382846285519
68-69	0.08684302052935437
70-71	0.12075237113058596
72-73	0.14422516313590444
74-75	0.18710086788755473
76-77	0.18684216238427032
78-79	0.14901436989182829
80-81	0.08703231723907467
82-83	0.12653854055770344
84-85	0.15456707337695738
86-87	0.16552104297943934
88-89	0.154314677763997
90-91	0.17465145427828233
92-93	0.09660442086059837
94-95	0.3278745210162255
96-97	0.3294583034875521
98-99	0.36785398610915504
100	0.3700750675032067
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7924068.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.4577527444639
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.38317591512937	26.664503422648828
2	12.292214240441284	8.47124157958208
3	3.8400627497224735	3.9695979825948955
4	1.7836511885700679	2.4584244655246628
5	0.9826121119691297	1.692930259897388
6	0.637304573703287	1.3176050054150301
7	0.47150763973352394	1.1372965566944475
8	0.33001085282980075	0.9097145895839154
9	0.23960206665406084	0.7430533892845369
>10	1.6498015164794784	10.965882621706116
>50	0.19257478632346933	4.6579815756062315
>100	0.16317825985043133	11.334957385073313
>500	0.020486695404667795	4.845564495752339
>1k	0.011901389946921017	7.679355482353242
>5k	7.737745785536378E-4	1.938378853377981
>10k+	0.0011422386635791797	11.213512334905033
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	117754	1.4860296504270283	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	105244	1.3281561945202893	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	48014	0.6059261480340653	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	47788	0.6030740776076127	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	42800	0.5401266117352855	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	41971	0.5296648135780764	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	38530	0.48624014836823715	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	38372	0.48424622302584985	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	37549	0.4738601435525288	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	34963	0.44122539079674733	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	30596	0.386114808706841	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	25869	0.3264611055836472	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25154	0.31743796242031236	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	20660	0.2607246681881074	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	18449	0.23282233317533368	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC	17645	0.22267602953432505	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15905	0.20071761120676904	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	15655	0.19756266604476386	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC	14919	0.18827450748782065	No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	14338	0.18094241493132063	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT	13892	0.17531399276230342	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	13297	0.16780522327673109	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	13027	0.1643978825017655	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12367	0.15606882727407184	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	12332	0.15562713495139113	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG	12265	0.15478160964797374	No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	11539	0.14561964889751072	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA	11021	0.13908260252183602	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	10704	0.13508213205641345	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	10505	0.13257079570745733	No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA	10077	0.12716952959010447	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC	9836	0.12412816245393149	No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	9776	0.12337097561505024	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	9475	0.11957242163999603	No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG	8910	0.11244224557386434	No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	8658	0.10926206085056311	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG	8425	0.1063216519595743	No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC	7981	0.10071846935185312	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2619780648020687E-4	0.0	0.0	0.0	0.0
2	2.0191649036833102E-4	2.5239561296041377E-5	0.0	2.5239561296041377E-5	0.0
3	2.0191649036833102E-4	2.5239561296041377E-5	0.0	2.5239561296041377E-5	0.0
4	2.5239561296041375E-4	3.7859341944062066E-5	0.0	2.5239561296041377E-5	0.0
5	0.001337696748690193	3.7859341944062066E-5	0.0	2.5239561296041377E-5	0.0
6	0.0026122945941402824	3.7859341944062066E-5	0.0	2.5239561296041377E-5	1.2619780648020689E-5
7	0.0028646902071006964	3.7859341944062066E-5	0.0	3.7859341944062066E-5	1.2619780648020689E-5
8	0.0033821012136695445	6.309890324010344E-5	0.0	5.0479122592082755E-5	1.2619780648020689E-5
9	0.0036723561685740205	7.571868388812413E-5	0.0	5.0479122592082755E-5	1.2619780648020689E-5
10-11	0.006322510104658365	7.571868388812413E-5	0.0	8.202857421213447E-5	1.2619780648020689E-5
12-13	0.012758598235148917	7.571868388812413E-5	0.0	1.577472581002586E-4	3.7859341944062066E-5
14-15	0.020444044649793512	1.2619780648020687E-4	0.0	2.2715605166437238E-4	6.309890324010344E-5
16-17	0.023510651347262542	1.2619780648020687E-4	0.0	4.290725420327034E-4	6.309890324010344E-5
18-19	0.025006095354052994	1.2619780648020687E-4	0.0	5.994395807809828E-4	6.309890324010344E-5
20-21	0.03082381423279053	1.2619780648020687E-4	0.0	9.591033292495723E-4	6.309890324010344E-5
22-23	0.03302596595587014	1.5143736777624826E-4	6.309890324010344E-6	0.0023725187618278895	6.940879356411378E-5
24-25	0.047507164249473884	1.6405714842426895E-4	1.2619780648020689E-5	0.005559013375453113	8.202857421213447E-5
26-27	0.09235155478221539	1.7667692907228963E-4	1.2619780648020689E-5	0.010764672892761647	8.833846453614481E-5
28-29	0.10222653313929159	1.7667692907228963E-4	1.2619780648020689E-5	0.0330575154074902	8.833846453614481E-5
30-31	0.1041952189203828	2.145362710163517E-4	1.2619780648020689E-5	0.09490075047311558	8.833846453614481E-5
32-33	0.1058357904046255	2.145362710163517E-4	1.2619780648020689E-5	0.188482733868513	8.833846453614481E-5
34-35	0.10696526077262336	2.713252839324448E-4	1.2619780648020689E-5	0.3066417400759307	8.833846453614481E-5
36-37	0.10895918611501063	3.281142968485379E-4	1.2619780648020689E-5	0.48508543843894325	8.833846453614481E-5
38-39	0.11110454882517415	3.407340774965586E-4	1.2619780648020689E-5	0.7517792628735644	8.833846453614481E-5
40-41	0.11294703679978516	4.416923226807241E-4	1.2619780648020689E-5	1.1336538252826704	8.833846453614481E-5
42-43	0.11761004574922881	4.606219936527551E-4	1.2619780648020689E-5	1.4135353204944732	8.833846453614481E-5
44-45	0.12353503276347452	5.111011162448379E-4	1.2619780648020689E-5	1.6975119345265588	8.833846453614481E-5
46-47	0.12615363724793882	5.426505678648896E-4	1.2619780648020689E-5	2.0303460293374567	8.833846453614481E-5
48-49	0.12776265928056146	5.489604581889E-4	1.2619780648020689E-5	2.333788655019114	8.833846453614481E-5
50-51	0.1299963604552611	6.183692517530137E-4	1.2619780648020689E-5	2.6766049963225957	8.833846453614481E-5
52-53	0.13091760444256662	6.309890324010344E-4	1.2619780648020689E-5	3.078822645136311	8.833846453614481E-5
54-55	0.1335425188173549	6.309890324010344E-4	1.2619780648020689E-5	3.4345490220427184	8.833846453614481E-5
56-57	0.13996598716719744	6.436088130490551E-4	1.2619780648020689E-5	3.8054380653977224	9.464835486015517E-5
58-59	0.1421870685612491	6.436088130490551E-4	1.2619780648020689E-5	4.195294639066701	1.0095824518416551E-4
60-61	0.14361941366479944	6.436088130490551E-4	1.2619780648020689E-5	4.555141374354688	1.0095824518416551E-4
62-63	0.14461006644566907	6.562285936970758E-4	1.2619780648020689E-5	4.927929694697219	1.2619780648020687E-4
64-65	0.1452915346006622	7.193274969371792E-4	1.2619780648020689E-5	5.330589035833615	1.2619780648020687E-4
66-67	0.14635790606541993	7.193274969371792E-4	1.2619780648020689E-5	5.747306812611906	1.5143736777624826E-4
68-69	0.14766405336249006	7.193274969371792E-4	1.2619780648020689E-5	6.243996139356704	1.5143736777624826E-4
70-71	0.14911532813701245	7.256373872611896E-4	1.2619780648020689E-5	6.843076056389219	1.7667692907228963E-4
72-73	0.15327354586053527	7.319472775851999E-4	1.2619780648020689E-5	7.339550846863	1.7667692907228963E-4
74-75	0.15738759435179	7.382571679092102E-4	1.2619780648020689E-5	7.814174487144735	1.7667692907228963E-4
76-77	0.15874422077145223	7.634967292052516E-4	1.2619780648020689E-5	8.33202087614594	1.7667692907228963E-4
78-79	0.1596276054168137	7.824264001772827E-4	1.2619780648020689E-5	8.841740126409817	1.7667692907228963E-4
80-81	0.16100316150744795	7.824264001772827E-4	1.2619780648020689E-5	9.41782680310164	1.829868193963E-4
82-83	0.16166569999146901	7.824264001772827E-4	1.2619780648020689E-5	10.082920792703948	1.8929670972031034E-4
84-85	0.16449884074694968	7.887362905012931E-4	1.2619780648020689E-5	10.69583829921702	1.8929670972031034E-4
86-87	0.17014619258693892	8.202857421213448E-4	1.2619780648020689E-5	11.304578658335593	2.0822638069234136E-4
88	0.17132614207752886	8.329055227693654E-4	1.2619780648020689E-5	11.795973987098545	2.2715605166437238E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCTAC	7240	0.0	29.212872	1
ATCTACA	8345	0.0	25.732452	2
TCTACAC	9540	0.0	24.682808	3
TACACTC	9285	0.0	24.625778	5
CTACACT	11775	0.0	22.270481	4
AGTACTT	29125	0.0	19.76159	12-13
CACTCTT	11615	0.0	19.270237	7
GAGTACT	28295	0.0	18.754835	12-13
CATGGGG	15700	0.0	18.74199	4
GTACTAG	1995	0.0	17.94474	1
ACTCTTT	14885	0.0	17.814428	8
GTATAAG	2540	0.0	17.803444	1
AGTACTC	2785	0.0	17.568869	5
TATCACG	520	0.0	17.206514	2
GTATATA	1670	0.0	17.205997	1
ACACTCT	13720	0.0	16.757427	6
TACTTTT	34760	0.0	16.698936	14-15
GTATTAG	3530	0.0	16.680222	1
CGTTAAC	795	0.0	16.59042	1
GTACTTT	32535	0.0	16.433016	14-15
>>END_MODULE